Digital Commons Network^™

Lipidomic And Proteomic Insights From Extracellular Vesicles In The Postmortem Dorsolateral Prefrontal Cortex Reveal Substance Use Disorder-Induced Brain Changes, Chioma M Okeoma, Wasifa Naushad, Bryson C Okeoma, Carlos Gartner, Yulica Santos-Ortega, Calvin Vary, Savio Lima-Bastos, Victor Corasolla Carregari, Martin R Larsen, Alessio Noghero, Consuelo Walss-Bass, Rodrigo Grassi-Oliveira

Faculty, Staff and Student Publications

Substance use disorder (SUD) significantly increases the risk of neurotoxicity, inflammation, oxidative stress, and impaired neuroplasticity. The activation of inflammatory pathways by substances may lead to reactive astrogliosis and chronic neuroinflammation, potentially mediated by the release of extracellular particles (EPs), such as extracellular condensates (ECs) and extracellular vesicles (EVs). These particles, which reflect the physiological, pathophysiological, and metabolic states of their cells of origin, might carry molecular signatures indicative of SUD. In particular, our study investigated neuroinflammatory signatures in SUD patients by isolating EVs from the dorsolateral prefrontal cortex (dlPFC) Brodmann's area 9 (BA9) from postmortem subjects. We isolated BA9-derived …

Rna’S Symphony: Harmonizing Splice Junctions And Exon Counts For A Novel Approach To Differential Splicing Analysis, Jelard Aquino

UNLV Theses, Dissertations, Professional Papers, and Capstones

Alternative Splicing (AS) plays a critical role in transcriptome complexity and cell-type-specific gene regulation, yet its analysis remains methodologically fragmented, especially in the context of noisy and sparse single-cell RNA sequencing (scRNA-seq) data. This dissertation addresses key computational challenges in AS detection by evaluating existing tools, developing integrative frameworks, and proposing new strategies for improving analysis accuracy in both bulk and single-cell contexts. In chapter 1, I present a comprehensive literature review of computational tools designed for detecting and quantifying AS from bulk and scRNA-seq data. This review outlines major methodological paradigms, including exon-based and splice junction-based approaches, and evaluates …

Exploring The Processing Of Objects, Settings, And Global Properties In Natural Auditory Scenes, Margaret A. Mcmullin

UNLV Theses, Dissertations, Professional Papers, and Capstones

Our auditory system has a remarkable ability to make sense of complex environments, allowing us to identify what we are hearing and where sounds come from. This process of auditory scene analysis is essential for situational awareness, decision-making, and effective communication in daily life. While much is known about how listeners segregate and identify individual sound sources (e.g., voices), less is understood about how global properties of a scene (e.g., openness, naturalness) contribute to perception. This dissertation examined how object- and setting-level information in natural auditory scenes are processed, and whether they rely on distinct or overlapping mechanisms. In Experiment …

Tractors On The Mall: Unraveling The New Deal, Political Shifts, And The 1980s Farm Crisis, Tracy M. Neblina

UNLV Theses, Dissertations, Professional Papers, and Capstones

In the 1980s, Midwestern farmers faced an economic crisis that rivaled the Great Depression. Agricultural policy in the 1970s and 1980s led to a boom and bust for many of the nation’s farmers, causing the decline in rural communities in the middle of the country. During this period, more efficient agricultural production, shifting federal policy, high inflation, and devalued farmland led farmers in the Midwest to face incredible economic hardships, loss of land, changes in their communities, and challenges to their sense of purpose and identity. The Farm Crisis of the 1980s furnishes an extraordinary opportunity to investigate a confluence …

Diverse Cell Types Establish A Pathogenic Immune Environment In Peripheral Neuropathy, Julie Choi

Arts & Sciences Graduate Student Theses and Dissertations

Neuroinflammation plays a complex and context-dependent role in many neurodegenerative diseases. We identified a key pathogenic function of macrophages in a mouse model of a rare human congenital neuropathy in which SARM1, the central executioner of axon degeneration, is activated by hypomorphic mutations in the axon survival factor NMNAT2. Macrophage depletion blocked and reversed neuropathic phenotypes in this sarmopathy model, revealing SARM1-dependent neuroimmune mechanisms as key drivers of disease pathogenesis. In this study, we investigated the impact of chronic subacute SARM1 activation on the peripheral nerve milieu using single cell/nucleus RNA-sequencing (sc/snRNA-seq). Our analyses reveal an expansion of immune cells …

Kinase Suppressor Of Ras 2 Regulates Energy Balance And Tumor Initiation, Dianna H. Huisman

Theses & Dissertations

Kinase Suppressor of Ras 2 (KSR2) is a scaffold that facilitates Raf-MEK-ERK signaling and is expressed in the brain, pituitary, and adrenal gland. Here, we demonstrate that KSR2 functions in the adult brain to maintain organismal energy balance and in small-cell lung carcinoma (SCLC) to promote self-renewal and clonogenicity. Disruption of KSR2 in adult mice results in hyperphagia, obesity, reduced metabolic rate, and altered activity. Using spatial transcriptomics, we identify regions of the brain associated with regulation of feeding and energy expenditure with altered gene expression following KSR2 disruption. These findings reveal regions of interest in which targeted KSR2 disruption …

Scientist Perspectives: A Correlation Analysis Of Translational Science Competencies Compared With Principles Of Engagement And Knowledge Integration, Rachel Wenzl

Theses & Dissertations

The competencies identified in the development of the “Fundamental Characteristics of the Translational Scientist” may not consider and fully integrate the concepts of knowledge integration and engagement, key components of the translational science spectrum. This gap may be widening and deepening the “valley of death” and exacerbating ongoing challenges in the field of translational science. This dissertation explored the association of knowledge integration, stakeholder engagement, and community engagement principles compared with translational science competencies.

Utilizing content review and a non-experimental, correlation study design, data were collected through a quantitative survey from adult research subjects all self-identified as a research investigator …

Analyzing Food Access: An Investigation Of Food Insecurity Amongst Latinos In Rural Nebraska, Natalia Santos

Theses & Dissertations

Background: Food insecurity is a public health issue in the United States, with disproportionately higher rates among Latino/Hispanic households. Rural communities face unique geographical, socioeconomic, and systemic barriers that often exacerbate food access disparities. Despite the growing Latino population in rural areas, particularly in Midwestern states like Nebraska, there remains a critical gap in comprehensive research that integrates both objective and subjective understandings of their food environments. Understanding these multifaceted influences is crucial for developing effective, culturally sensitive interventions.

Problem Statement: This dissertation aims to answer the central question: How does food access and food insecurity manifest amongst Latinos in …

Phosphorylation Deficient Inducible Camp Early Repressor (Icer) Modulates Tumorigenesis And Survival In A Transgenic Zebrafish (Danio Rerio) Model Of Melanoma, Justin Wheelan, Melissa Rose Spigelman, Angelo Cirinelli, James Reilly, Carlos A. Molina

Department of Biology Faculty Scholarship and Creative Works

Melanoma, the most lethal form of skin cancer, is commonly associated with mutations in the BRAF gene, particularly BRAF^V600E, which drives tumor proliferation via the ERK1/2 signaling cascade. While BRAF inhibitors initially demonstrate efficacy, therapeutic resistance remains a significant challenge. Emerging evidence implicates the cAMP signaling pathway, particularly the cAMP response element-binding protein (CREB) and its repressor, inducible cAMP early repressor (ICER), in melanoma progression and drug resistance. ICER, a transcriptional repressor regulated via Ras/MAPK-mediated phosphorylation and ubiquitination, is degraded in melanoma, undermining its tumor-suppressive role. In a braf^V600E; p53 (loss of function) transgenic zebrafish ( …

Large-Scale Crispr Screening In Primary Human 3d Gastric Organoids Enables Comprehensive Dissection Of Gene-Drug Interactions, Yuan-Hung Lo, Hudson T Horn, Mo-Fan Huang, Wei-Chieh Yu, Chia-Mei Young, Qing Liu, Madeline Tomaske, Martina Towers, Antonia Dominguez, Michael C Bassik, Dung-Fang Lee, Lei S Qi, Jonathan S Weissman, Jin Chen, Calvin J Kuo

Faculty, Staff and Student Publications

Understanding how genes influence drug responses is critical for advancing personalized cancer treatments. However, identifying these gene-drug interactions in a physiologically relevant human system remains a challenge, as it requires a model that reflects the complexity and heterogeneity among individuals. Here we show that large-scale CRISPR-based genetic screens, including knockout, interference (CRISPRi), activation (CRISPRa), and single-cell approaches, can be applied in primary human 3D gastric organoids to systematically identify genes that affect sensitivity to cisplatin. Our screens uncover genes that modulate cisplatin response. By combining CRISPR perturbations with single-cell transcriptomics, we resolve how genetic alterations interact with cisplatin at the …

Genome Sequences Of Eight Fusobacterium Watanabei Sp Isolates, Martha A Zepeda-Rivera, Falk Ponath, Kaitlyn N Lewis, Rutika P Gavate, Floyd E Dewhirst, Junko Tomida, Yoshiaki Kawamura, Kaori Tanaka, Susan Bullman, Christopher D Johnston

Faculty, Staff and Student Publications

We report draft genome sequences of eight Fusobacterium watanabei clinical isolates, ranging from 1.95 to 2.09 Mbp. Analysis against the Genome Taxonomy Database indicates that F. watanabei genomes are part of the "Fusobacterium nucleatum_J" group.

Proteomics-Based Aging Clocks In Midlife Or Late-Life And Their Associated Risk Of Dementia, Sanaz Sedaghat, Saeun Park, Rob F Walker, Shuo Wang, Jialing Liu, Timothy M Hughes, Behnam Sabayan, Weihong Tang, Josef Coresh, James S Pankow, Keenan A Walker, Ramon Casanova, Ruth Dubin, Rajat Deo, Jerome I Rotter, Alexis C Wood, Peter Ganz, Pamela L Lutsey, Weihua Guan, Anna Prizment

Children’s Nutrition Research Center Staff Publications

Background: Biological age can be quantified by composite proteomic scores, called proteomics-based aging clocks (PACs). We investigated whether a discrepancy between chronological and biological age in midlife and late-life is associated with cognition and dementia risk.

Methods: We used two longitudinal population-based studies: the Atherosclerosis Risk in Communities (ARIC) Study and the Multi-Ethnic Study of Atherosclerosis (MESA). PACs were created in ARIC at midlife (mean age: 58 years, 57% female, n = 11,758) and late-life (mean age: 77 years, 56% female, n = 4934) using elastic net regression models in two-thirds of dementia-free participants and validated in the remaining one-third …

The Lesson Horse: A Guide For Traditional Horsemanship & Equine-Assisted Services Programs 2nd Edition, Sarah Andersen

Animal, Dairy & Veterinary Sciences Textbooks

This practical guide equips equine professionals with the tools to select, assess, and develop safe, capable horses for traditional horsemanship and equine-assisted services programs. It introduces structured assessment tools to evaluate horse suitability and performance, supports the creation of individualized training plans, and addresses long-term horse welfare through continued evaluation and thoughtful end-of-work planning.

An Exciting Future For Microbial Molecular Biology And Physiology, Andrew A Bridges, Leah Guthrie, Mckenzie Lehman, Elizabeth H Kellogg, Samantha Wellington Miranda, Andrew Pountain, Anthony L Shiver, Andrew Varble, Molecular Biology And Physiology Community Of The Council On Microbial Sciences, Heidi B Kaplan, Elizabeth A Shank, Gisela Storz

Faculty, Staff and Student Publications

Continuous advances in technologies ranging from deep sequencing and genetic manipulation to mass spectrometry, single cell imaging, and structural biology have led to previously unimaginable advances in our understanding of microbial physiology and the molecular mechanisms underlying microbial responses in a multitude of environments. Simultaneously, these advances are revealing how much more there is to learn. At the 2024 virtual retreat of the Molecular Biology and Physiology (MBP) Community of the Council on Microbial Sciences (COMS) of the American Society for Microbiology (ASM), eight early-career investigators, along with retreat attendees, discussed some of these astounding advances, as well as the …

Csf-Exosomal Mirnas And Delayed Cerebral Ischemia: Insights Into Pathophysiology But No Definitive Biomarkers, Chathathayil M Shafeeque, Devin W Mcbride, Yuanqing Yan, Hussein A Zeineddine, John P Hagen, H Alex Choi, Jude P Savarraj, Ari Dienel, Spiros L Blackburn, Peeyush Kumar Thankamani

Faculty, Staff and Student Publications

Background: Aneurysmal subarachnoid hemorrhage (aSAH) is notoriously known for its high mortality and morbidity. Approximately one-third of the patients who survive aneurysm rupture are reported to develop delayed cerebral ischemia (DCI), which contributes to a poor clinical outcome. Currently, there are no biomarkers for identifying which aSAH patients are at risk of developing DCI. We aimed to determine the feasibility of cerebrospinal fluid (CSF) exosomal microRNAs (miRNAs) for predicting DCI post-aSAH.

Methods: aSAH patients were prospectively enrolled, and CSF samples were collected at two time points (< 24 h and 72 h post-aSAH) from individuals undergoing external ventricular drainage. Exosomal miRNAs were isolated from the CSF for analysis. In the initial group of patients (discovery cohort), an exploratory analysis was conducted using a CSF panel containing 84 miRNAs, assessed by quantitative real-time PCR (RT-qPCR). Based on this analysis, 27 miRNAs were selected for further evaluation in a second group of patients (validation cohort). Among these, 10 miRNAs had previously been reported in SAH-related CSF studies, supporting their relevance for continued investigation.

Results: In this study, RT-qPCR analysis of 84 miRNAs in CSF samples from …

Video-Based Intervention To Reduce Treatment And Outcome Disparities In Adults Living With Stroke Or Transient Ischemic Attack (Virtual): Protocol For A Randomized Controlled Trial, Munachi Okpala, Chigozirim Izeogu, Mengxi Wang, Charles Green, Gabretta Cooksey, Thuy Nguyen, Sarah Cohen, Latonya Bryant, Daphne C Hernandez, Elmer V Bernstam, Michael Gonzales, Rhonda Conyers, Olasimbo Chiadika, Kristin Varacalli, Sean I Savitz, Jose-Miguel Yamal, Anjail Z Sharrief

Faculty, Staff and Student Publications

Background: Racial and ethnic disparities in post-stroke blood pressure (BP) control persist, and effective interventions to address post-stroke care inequities are needed. We designed a randomized comparative effectiveness trial to evaluate the Video-based Intervention to Reduce Treatment and Outcome Disparities in Adults Living with Stroke or Transient Ischemic Attack (VIRTUAL) model of care for post-stroke BP reduction.

Methods: The study will enroll 534 stroke survivors in a randomized trial to receive either the VIRTUAL intervention or enhanced standard care. Individuals with ischemic stroke, hemorrhagic stroke, or transient ischemic attack (TIA) are enrolled before hospital discharge and randomized (1:1) to VIRTUAL …

Extracellular Vesicles For Clinical Diagnostics: From Bulk Measurements To Single-Vesicle Analysis, Hai Linh Tran, Wenshu Zheng, David A Issadore, Hyungsoon Im, Yoon-Kyoung Cho, Yuanqing Zhang, Dingbin Liu, Yang Liu, Bo Li, Fei Liu, David Tai Wai Wong, Jiashu Sun, Kun Qian, Mei He, Meihua Wan, Yong Zeng, Ke Cheng, Tony Jun Huang, Daniel T Chiu, Luke P Lee, Lei Zheng, Andrew K Godwin, Raghu Kalluri, Steven A Soper, Tony Y Hu

Faculty, Staff and Student Publications

Extracellular vesicles (EVs) play a crucial role in intercellular communication, signaling pathways, and disease pathogenesis by transporting biomolecules such as DNA, RNA, proteins, and lipids derived from their cells of origin, and they have demonstrated substantial potential in clinical applications. Their clinical significance underscores the need for sensitive methods to fully harness their diagnostic potential. In this comprehensive review, we explore EV heterogeneity related to biogenesis, structure, content, origin, sample type, and function roles; the use of EVs as disease biomarkers; and the evolving landscape of EV measurement for clinical diagnostics, highlighting the progression from bulk measurement to single vesicle …

Computational And Mathematical Modeling Of Microbial Community And Host Responses To Microbiota-Directed Complementary Foods Administered To Children With Acute Malnutrition., Steven J. Hartman

Arts & Sciences Graduate Student Theses and Dissertations

Globally, millions of children suffer from undernutrition, with stunting affecting 150 million and wasting nearly 50 million under five years old. Current interventions have shown limited effectiveness in mitigating long-term consequences such as stunted growth, cognitive deficits, and immune dysfunction. Through this body of work, I investigated the efficacy of a microbiota- directed complementary food (MDCF-2) as a next generation therapeutic food to combat childhood stunting. Across two randomized controlled trials involving Bangladeshi children with either moderate acute malnutrition or post-severe acute malnutrition, MDCF-2 significantly outperformed standard ready-to-use supplementary foods (RUSF), leading to significantly improved ponderal and sustained linear growth …

Optimization Of Rabbit Urine Liquid Organic Fertilizer And Reduced Npk Fertilizer Doses For Improving Growth And Physiology Of Shallot (Allium Ascalonicum L.), Etik Wukir Tini, Rifqi Raditya Kurniawan

Jurnal Kultivasi

Rabbit urine liquid organic fertilizer (LOF) can be used as an alternative fertilizer to reduce the use of inorganic fertilizers, especially for shallots, as a national food commodity. This study aims to determine the best concentration of rabbit urine LOF, the best dose of NPK fertilizer dose reduction, and a combination of rabbit urine LOF and NPK fertilizer dose reduction on the physiological characters of shallot plants. The observed variables consisted of net assimilation rate, crop growth rate, stomatal density, stomatal aperture, and chlorophyll content. The experimental results showed that a concentration of 200 mL L-1 of rabbit urine LOF …

Chemically Mediated Communication In Mound-Building Termites, Sree Subha Ramaswamy

Theses and Dissertations

Nest-building in social insects requires layered communication to coordinate complex, functional structures for housing, protection, storage, and homeostasis. This is especially striking in mound-building termites, which construct and repair large mounds collectively despite lacking image-forming eyes. This thesis investigates how Odontotermes obesus achieves such coordination through chemical communication embedded in soil.

Using controlled two-choice assays with manipulated soils, I separated airborne volatile signals from contact non-volatile signals by baking to remove volatiles, a mesh assay that presented volatile cues without contact, and solvent extraction with reapplication of contact cues. I quantified responses in major-only, minor-only, and mixed groups at two …

An Rna Modification Prevents Extended Codon-Anticodon Interactions From Facilitating +1 Frameshifting, Evelyn M. Kimbrough, Ha An Nguyen, Haixing Li, Jacob M. Mattingly, Nevette A. Bailey, Wei Ning, Howard Gamper, Ya-Ming Hou, Ruben L. Gonzalez, Christine M. Dunham

Department of Biochemistry and Molecular Biology Faculty Papers

RNA post-transcriptional modifications act by stabilizing the functional conformations of RNA. While their role in messenger RNA (mRNA) decoding is well established, it is less clear how transfer RNA (tRNA) modifications outside the anticodon contribute to tRNA stability and accurate protein synthesis. Absence of such modifications causes translation errors, including mRNA frameshifting. By integrating single-molecule fluorescence resonance energy transfer and cryogenic electron microscopy, we demonstrate that the N1-methylguanosine (m1G) modification at position 37 of Escherichia coli tRNAProL is necessary and sufficient for modulating the conformational energy of this tRNA on the ribosome so as to suppress +1 frameshifting otherwise induced …

Hippocampal Avoidance During Prophylactic Cranial Irradiation For Patients With Small Cell Lung Cancer: Randomized Phase Ii/Iii Trial Nrg-Cc003, Vinai Gondi, Stephanie L Pugh, Minesh P Mehta, Jeffrey S Wefel, Wolfgang A Tomé, Alexander Y Sun, John Grecula, Kristin J Redmond, Shannon Fogh, Laurie Gaspar, Andre Konski, Joseph Bovi, Clifford G Robinson, Benjamin Corn, Gregory M Videtic, Benjamin H Lok, Harold A Yoon, John H Heinzerling, Albert S Denittis, Ronald C Mcgarry, Kiran Devisetty, Vijayananda Kundapur, Abraham J Wu, Edward C Mccarron, Isabelle Thibault, Edmund L Simon, Andrew M Baschnagel, Samir Narayan, Jondavid Pollock, Rebecca Paulus, Lisa A Kachnic

Faculty, Staff and Student Publications

Purpose: Hippocampal avoidance (HA) during therapeutic whole-brain radiotherapy reduces the risk of neurocognitive function (NCF) toxicity in patients with brain metastasis. This trial hypothesized that HA during prophylactic cranial irradiation (PCI) in patients with small cell lung cancer (SCLC) leads to noninferior intracranial relapse (ICR) and reduction in NCF toxicity.

Methods: This randomized phase II/III trial enrolled patients with SCLC, no brain metastases, and response to chemotherapy. The primary end points were 12-month ICR (noninferiority design, randomized phase II) and 6-month Hopkins Verbal Learning Test-Revised (HVLT-R) Delayed Recall (DR) failure (phase III). Secondary end points were failure in any NCF …

From Larvae To Livelihoods: Examining The Realities Of Black Soldier Fly Farming In Malawi, Emily Herriott

Capstone Collection

Black Soldier Fly (BSF) larvae offer a cost-effective, nutrient-rich alternative to synthetic fertilizers and conventional feed inputs such as soy, fishmeal, and maize bran in sub-Saharan Africa. In Malawi, where smallholder farmers grapple with declining soil fertility, high input prices, chronic food insecurity, and livestock nutritional challenges, BSF farming is emerging as a promising circular agricultural innovation. Despite its technical promise, the social, economic, and ecological realities of BSF adoption remain underexplored.This study investigates the early implementation of BSF farming in Malawi through the Sustainable Livelihoods Framework, focusing on farmer experiences, challenges, and the social and structural processes shaping uptake …

Integrating Neuroaesthetic Design Into Brain-On-Chip Research: Toward Enhanced Neural Engagement And Cognitive Accessibility, Piper Hutson, James Hutson

Faculty Scholarship

This study examines the potential for neuroaesthetic design and adaptive neurofeedback principles to enhance brain-on-chip (BoC) research, with an emphasis on optimizing neural engagement and cognitive accessibility for diverse populations. Existing BoC platforms have transformed neuroscience through high-fidelity modeling of neural circuits, yet cognitive accessibility and sensory inclusion remain comparatively underexplored. Drawing upon recent studies in neuroinclusion, multisensory interface design, and adaptive feedback, this work synthesizes findings from biophilic interface architecture, neurofeedback literature, and embodied cognition frameworks. The manuscript outlines a multidisciplinary research agenda that prioritizes sensory diversity and ethical considerations in the evolution of BoC systems. Instead of making …

Growth And Yield Of Curly Red Chili (Capsicum Annum L.) In Response To Mulching And Chicken Manure Application In The Erosion-Prone Area Of Loto, Ternate Island, Tri Mulya Hartati, Lily Ishak, Krishna Aji, Chumidach Roini

Jurnal Kultivasi

Information on soil resources in volcanic landscapes presents a high potential for agricultural land development, but its development is always faced with the potential for continuous land degradation. This study aims to reduce the rate of land degradation through the application of vegetative conservation in erosion-prone Loto agrotourism area, Ternate Island. The research location is focused on erosion-prone areas. The research consisted of a soil resource inventory and a vegetative conservation application. The vegetative conservation application method used a Randomized Block Design (RBD) pattern, composed of 2 factors. The first factor is mulching, namely: no mulching (M0) and mulching (M1), …

Chelidonium Majus L.-Containing Gel May Improve Diabetic Wound Healing By Modulating Mmp-2, Mmp-9, And Collagen Levels, Kaan Kaltalioğlu, Eli̇f Naz Gürsoy, Barbaros Balabanli, Zeki̇ Aytaç, Şule Coşkun Cevher

Turkish Journal of Biology

Background/aim: Delayed wound healing in diabetic patients is a significant complication that reduces quality of life, prompting the continuous investigation of new therapeutic agents. This study designed to explore the dose-dependent effects of different parts of Chelidonium majus L. (CM), a medicinal plant traditionally used for skin disorders, on diabetic skin wounds.

Materials and methods: In diabetic rats, full-thickness excisional wounds were formed. CM-containing gels (aerial parts at 3%, 6%, 9% and root at 0.5%, 1%, 1.5%) were developed and applied to the wounds. After the treatment period, the rats were sacrificed, and wound healing activity was assessed macroscopically, histopathologically, …

Rice (Oryza Sativa L.) Production, Productivity, And Harvested Area In Karawang Regency Under Extreme Weather From 1991-2023, Adine Syabina Buswar, Ruminta, Fiky Yulianto Wicaksono

Jurnal Kultivasi

Climate change occurred in Karawang Regency due to extreme weather events, which are included in the El Nino climate anomaly phenomenon. The existence of extreme weather events in Karawang Regency has decreased rice production, which is a climate-dependent crop. Based on this problem, a study was conducted to analyze the identification of extreme weather changes and correlation analysis of rice crops in Karawang Regency to see the magnitude of extreme weather changes and the influence of extreme weather elements on rice crops. The method used is a quantitative descriptive method by analyzing extreme weather changes, correlation analysis of extreme weather …

Different Hypoxic Response Of Human Cavii And Caenorhabditis Elegans Homologous Genes Cah-3, Cah-4, Cah-5, Sümeyye Aydoğan Türkoğlu, Aysu Bozkurt, Fatma Poyrazli, Derya Okuyan

Turkish Journal of Biology

Background/aim: A number of carbonic anhydrase (CA) family proteins have been implicated in cancer. They contribute to the hypoxic microenvironment. CAVII is often downregulated in colorectal carcinoma and it has been associated with increased tumor size, node metastasis, and adverse clinical outcomes. In this study, we aimed to investigate the effect of hypoxia on CAVII protein in human colon cancer and prostate cancer cells. In addition, the regulation of CAH genes in Caenorhabditis elegans was examined. These are homologous to CAVII in humans.

Materials and methods: CAVII expression was analyzed in different cell lines such as human colon cancer (SW480 …

Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang

Turkish Journal of Biology

Aim: Neuronal cell death plays a critical role in the development of neurological disorders associated with aging. This study aimed to evaluate the beneficial effects of heat-killed lactic acid bacteria (hkLAB) on neuroblastoma cells and Caenorhabditis elegans.

Materials and methods: We pretreated heat-killed Lactobacillus fermentum CNU384 (hkCNU384), L. brevis CNU386 (hkCNU386), L. helveticus CNU395 (hkCNU395), and L. paracasei CNU396 (hkCNU396) at a concentration of 10⁹ CFU/mL to investigate their neuroprotective effects on glutamate-induced SH-SY5Y cells and their impact on the lifespan of 6-hydroxydopamine (6-OHDA)-induced C. elegans.

Results: Our findings indicate that hkCNU395 and hkCNU396 protected against glutamate-induced cell death …

Recreational Boat Fishing Participation Varies Across Bioregional Fisheries In Western Australia, With Persistent Patterns Within Fisheries And Fisher Typologies Over Time, Karina L. Ryan, Eva Lai, Claire B. Smallwood, Jeremy M. Lyle, Kate E. Stark, Sean R. Tracey

Fisheries Research Articles

Recreational fishing is popular worldwide and understanding variability in participation can inform policies that seek to improve sustain[1]ability of recreational fisheries and amenity for recreational fishers. However, not all recreational fishers are alike in their demographics or behaviours. This study explores temporal and spatial variability in licensed boat-based recreational fishing participation in Western Australia using data from a consistent survey design delivered 12 times from 2011 to 2024. Five fisher participation typologies were identified across four marine bioregions (North Coast, Gascoyne Coast, West Coast, and South Coast) and four fishery types (demersal, nearshore, pelagic, and invertebrate) with varied fisher characteristics …