Digital Commons Network^™

Assessing The Human Health Impacts Of Nutrient-Dense Diets: Clinical Trials On Regenerative Agriculture And Protein Source, Jennifer Jensen-Cloward

All Graduate Theses and Dissertations, Fall 2023 to Present

Increasing consumer demand for sustainable, animal-friendly food options has sparked discussion about farming production practices. As more people prioritize their food choices, questions have circulated about how farming practices might impact not just the environment, but also human health. Regenerative agriculture is a farming method that has gained popularity for its potential to improve both food quality and environmental outcomes. It focuses on restoring ecosystem function, ultimately impacting the resulting nutrients in food products, and includes foods like grass-fed meat and organic produce. However, it is still unclear whether these practices make a difference in human health. This dissertation informs …

Why The Long Face? The Distribution And Niche Of The Imperiled Narrow-Faced Kangaroo Rat (Dipodomys Venustus), Garrett L. Gimbel

Master's Theses

Distribution models provide a practical use case of connecting ecological niche theory with species’ occurrence and assemblages. By explicitly linking Hutchinson’s “niche space” with geographic space researchers can gain valuable information on a species’ niche characteristics and interactions, thereby informing conservation and management practices. The narrow-faced kangaroo rat (Dipodomys venustus) is a highly understudied kangaroo rat species endemic to the central coast of California and is an ideal species to explore niche theory in distribution modeling. Three subspecies are currently recognized: The Santa Cruz kangaroo rat (D. v. venustus), the elephant-eared kangaroo rat (D. v. …

An Update On Novel Taxa And Revised Taxonomic Status Of Bacteria Isolated From Human Clinical Specimens Described In 2024, Arianna Carella, Karen C. Carroll, Erik Munson

Clinical Lab Sciences Faculty Research and Publications

Knowledge of novel and revised bacterial taxonomy facilitates routine operations in the medical microbiology laboratory and communication with important stakeholders. Notable new gram-positive taxa accepted in 2024 include the clinically significant Staphylococcus brunensis sp. nov., as well as Streptococcus suis subsp. hashimotonensis subsp. nov., which can be delineated from other Streptococcus suis subsp. by possession of the Lancefield group A antigen. Four novel Enterobacterales species are discussed, plus a novel eighth family Gallaecimonadaceae. One of these, Providencia huashanensis sp. nov. is clinically significant and harbors multiple genetic determinants that encode antimicrobial resistance mechanisms. Stenotrophomonas forensis sp. nov., derived from …

Effects Of Injectable Trace Mineral Timing On Calf Performance And Health Indicators, Marie E. Goulais

Graduate Theses and Dissertations

One hundred and fourteen calves (average BW= 224kg ± 89kg) were utilized to evaluate the effects of injectable trace mineral (ITM) administration on calf performance and health when administered pre-weaning and at weaning. Calves were blocked by sex, stratified by age, and randomly assigned to one of the following treatment groups: 1) no ITM injection (CON), 2) ITM administered 28 d before weaning (PW), or 3) ITM administration at weaning (WEAN). Calves were vaccinated against respiratory pathogens on D-28 and D0. At weaning, cattle were transported to a local auction market, held overnight and returned the following morning. Body weight …

Examing Fear Conditioning/Extinction And The Role Of The Amygdala In Human Neuroimaging., Olivia Cook

Electronic Theses and Dissertations

Fear plays a crucial role in survival but can lead to psychological disorders when dysregulated. Fears can be innate due to evolutionary relevance or learned through associative processes, where predictive accuracy of threat is pivotal. Disruptions in fear neurocircuitry contribute to anxiety disorders, characterized by heightened activation and impaired learning. Fear conditioning and extinction paradigms based on classical Pavlovian conditioning have been used to study mechanisms underlying fear learning. Using this experimental model, fear networks in rodents have been described in detail with clear distinction between conditioning and extinction neural functioning. Yet, there is no consensus on the neurobiological processes …

Characterizing The Ethanol Sensitivity Of The Planar Cell Polarity Pathway In Endoderm Morphogenesis And Jaw Development., Raéden Omneil C. C. Rolle Gray

Electronic Theses and Dissertations

Fetal Alcohol Spectrum Disorders (FASD) describes a large range of developmental defects due to prenatal alcohol exposure. It is characterized by neural and facial defects, including jaw hypoplasia. Many factors contribute to the susceptibility of ethanol-induced facial defects, including genetics, yet the genes involved, and the cell events they regulate, are poorly understood. Zebrafish have a large number of tools for genetic manipulation available, share 70% of genes with humans, are fertilized externally and are translucent during embryogenesis, making them great models to study FASD. Previous studies show that two members of the Planar Cell Polarity (PCP) pathway, zebrafish vangl2 …

Live And Let Fly: Assessing Threats To California Condors, Molly Astell

Boise State University Theses and Dissertations

California condors (Gymnogyps californianus) are a critically endangered species that face threats, both anthropogenic and natural, that slow recovery of their population. The leading causes of death of condors are lead toxicosis, predation, trauma, fire, and collision and electrocution at power lines. As a long-lived and slow-maturing species, each death is consequential and understanding when and where threats occur can greatly benefit management and mitigation. Although the focus of extensive mitigation efforts, lead toxicosis remains the leading cause of death for condors. There has been less effort dedicated to addressing the second-leading anthropogenic threat, interactions with power lines. Such efforts …

Modeling The Deleterious Effects Of An Endogenously Generated Apolipoprotein-E4 Fragment In Transgenic Zebrafish, Emily Egan

Boise State University Theses and Dissertations

Harboring the apolipoprotein E4 allele (APOE4) is the strongest genetic risk factor for late-onset Alzheimer’s disease, yet the mechanisms by which the expressed protein contributes to neurodegeneration remain unknown. Previous studies suggest that proteolytic cleavage of ApoE4 generates amino-terminal fragments that are neurotoxic and may drive cellular dysfunction. To investigate the deleterious effects of one such fragment, nApo_E1-151, in a living vertebrate model, we developed a transgenic zebrafish line using Tol2-mediated transgenesis to endogenously express nApo_E1-151 under the neuron-specific HuC/D promoter. This model utilizes the zebrafish’s genetic accessibility, embryo and larva transparency, and rapid development ex utero to study the …

Shifting Grounds: Knowledge, Power, And Place In Changing Western Rangelands, Molly C. Levy

Boise State University Theses and Dissertations

Public rangelands in the American West are sites of enduring contestation, where ecological change, competing values, and institutional complexity converge. Long-standing debates over livestock grazing, wildlife conservation, and land stewardship highlight fundamental questions of whose knowledge counts, whose authority is legitimate, and how resource use should adapt under shifting social and ecological conditions. This dissertation examines the intersections of knowledge, power, and land use in collaborative governance of contested landscapes. Using the Thunder Basin ecoregion (TBER) in northeastern Wyoming as a case study, I adopt a mixed-methods approach that integrates qualitative interviews, participant observation, archival research, and quantitative analysis of …

Assessing Overtopping Hazard To The Dawlish Railway Line: Integrating Wave Propagation Models For Coastal Metocean Condition Analysis, Matthew R. Owen

The Plymouth Student Scientist

With rising sea levels and increasingly frequent and intense storms due to climate change, coastal communities and structures are at an increasing risk of overtopping occurring. This study investigates the coastal metocean conditions responsible for overtopping at coastal defences, using Delft3D to model these processes with Dawlish as the case study site. Wave propagation models were employed to simulate metocean conditions from December 2021 to March 2022 to compare inshore wave conditions, alongside water level and wind parameters, with observed overtopping data from the WireWall system installed on top of the seawall, next to the railway line. The results indicate …

From Sea To Shore: Wind-Driven Aerosolization Of Harmful Algal Bloom Toxins In Qingdao, China, During The Winter Of 2019, Madeleine K. Martin

The Plymouth Student Scientist

In Qingdao, China, harmful algal blooms (HABs) are a known hazard to coastal communities. Aerosolization is a mechanism by which HAB toxins can be transported from the sea surface into the air when wind speeds exceed 5 m/s. Previous studies have analysed the composition of aerosolized marine biotoxins (AMBs) in the breathable air surrounding Qingdao, but the role of the onshore winds in transporting them there would benefit from further research. Ciguatoxin is one such AMB, and this study analyses the role of onshore winds in aerosolizing and transporting toxins inland from a ciguatoxin bloom in Aoshan Bay, Qingdao during …

Comparing The Effect Of Marine Aquarium Salts And Sodium Chloride On Vicia Faba Stress Response, Bethany Smerdon

The Plymouth Student Scientist

Soil salinity is a major abiotic stressor limiting agricultural productivity. Many salt stress studies rely solely on sodium chloride (NaCl) solutions, despite not accurately representing natural saline conditions. This study investigated the stress response of Vicia faba to NaCl and marine aquarium salt (MAS) solutions to assess the impact of a more complex ionic composition on plant physiology. Plants were cultivated in a controlled greenhouse and treated with either NaCl, MAS, or rainwater (control) for up to 55 days. Growth parameters, water status, proline accumulation, chlorophyll content, and root nodule weight were measured to evaluate stress responses. The results indicate …

Assessing Jasmonic Acid Seed Priming In Brassica Napus: Impacts On Aphid Herbivory Resistance And Seedling Fitness Trade-Offs, Daisy Stradling

The Plymouth Student Scientist

Jasmonic acid (JA) is a key phytohormone involved in plant defence, offering a potential sustainable alternative to conventional chemical pesticides. While JA seed priming has been explored as a strategy to enhance herbivore resistance in certain plant families, its potential fitness trade-offs remain poorly understood. This study investigates the effects of JA seed priming on Brassica napus (oilseed rape), a key agricultural crop, focusing on seedling fitness and resistance to Brevicoryne brassicae (mealy cabbage aphid), a major agricultural pest. To assess potential fitness trade-offs, seeds were primed with 75 µM JA, and multiple seedling fitness parameters were measured. Germination monitoring …

Collective Agency And Community Resilience Among Farmworkers In The Lower Rio Grande Valley, Angel Velazquez

Theses and Dissertations

This research assesses how farmworkers in the Lower Rio Grande Valley of Texas use everyday practices to build resilience and adapt to changes in their lives. I apply two interconnected theories, White’s (2017, 2019) framework of collective agency and community resilience and Castro and Sen’s (2022) framework of everyday adaptation actions, to study these practices. I conducted 19 semi-structured interviews with Latino farmworkers and professionals who work with local farmworker organizations in the Rio Grande Valley (RGV) of Texas. Interview transcripts were analyzed to determine if and how Latino farmworkers in the RGV can work towards community resilience and implement …

Bacillus Subtilis: A Bacterium That Can Potentially Degrade Low-Density Polyethylene (Ldpe) And Polyethylene Terephthalate (Pet), Migdalia Flores

Theses and Dissertations

The widespread application and disposal of plastics, such as low-density polyethylene (LDPE) and polyethylene terephthalate (PET), have raised significant environmental health concerns. Because of each plastic’s stability and durability, these plastics exhibit limited natural degradation in the environment. Recently, the environmentally friendly biodegradation of plastics by living microorganisms has attracted more attention, as they can assist in the natural degradation process. In this study, the bacterial strain Bacillus subtilis ATCC6051 was used to degrade LDPE and PET for 60 days. To measure the degradation of low-density (LDPE) and polyethylene terephthalate (PET), the dry weight was taken, and physical characteristics were …

Population Structure Of Red Drum (Sciaenops Ocellatus (Linnaeus, 1766)) In Two Systems In The Northwestern Gulf Of Mexico, Sandra Antonia Leal

Theses and Dissertations

Red drum (Sciaenops ocellatus) are an economically significant species of fish inhabiting the Gulf of Mexico. There are limited studies assessing S. ocellatus population structure focused on the southern coast of Texas. A total of 1,008 fish were collected over one year on a quarterly basis at South Bay (SB) and Holly Beach (HB) in the Lower Laguna Madre (LLM) of Texas to assess population structure. Juvenile S. ocellatus utilize the water systems of the LLM as a nursery ground and were more abundant at SB (n = 718) than HB (n = 290). The most abundant age classes were …

Ontogenetic Analysis Of Parasaurolophus Braincases (Ornithischia, Hadrosauridae) From The Kaiparowits Formation (Upper Cretaceous) Of Utah, Usa: Comparing Juvenile And Adult Morphology, Amanda "Billie" C. Guerrero

Electronic Theses, Projects, and Dissertations

This study documents the ontogenetic development of the braincase in Parasaurolophus, a lambeosaurine hadrosaurid dinosaur known for its distinctive cranial crest. Whereas the crest has been extensively examined for its roles in communication and display, the braincase remains less understood, despite its structural and functional relationship with the crest. This research compares the braincase morphology of a juvenile specimen (RAM 14000, "Baby Joe"), a subadult specimen (RAM 17000), and an adult P. tubicen (NMMNH P-25100), to investigate how developmental changes influenced sensory systems, neurocranial architecture, and biomechanical adaptations across ontogeny.

Detailed morphological analysis reveals progressive changes from juvenile to …

Reproduction And Larval Development Of The Invasive Asian Longhorned Beetle In South Carolina, Lindsey A. Stone

All Theses

The Asian longhorned beetle (Anoplophora glabripennis Motschulsky; ALB) is a major threat to forest health due to the damage done by larval feeding in host trees. In 2020, ALB was discovered in Charleston County, South Carolina, which presents a novel climate for this invasive pest in North America. I investigated the reproductive biology and larval development of ALB to support detection and management efforts. By exposing 19 host trees to mated pairs of adults, I was able to determine rates of oviposition success (55%), egg viability (55%), and early larval survival (56%). On average, females chewed nearly 1 egg …

Investigating The Impacts Of Azole Antifungals On Growth Of Cryptococcus Neoformans, Gnanambika Sindhe

All Theses

Cryptococcus neoformans, an opportunistic fungal pathogen is responsible for cryptococcosis, leading to approximately 181,000 deaths annually, predominantly in regions such as Sub-Saharan Africa. Fluconazole, a widely used fungistatic azole, is often administered due to its relative affordability and lower toxicity. However, resistance to fluconazole in C. neoformans can arise through aneuploidy, complicating treatment efforts. This study aims to deepen our understanding of resistance mechanisms to azole drugs by comparing fluconazole with other azoles, including both clinical and agricultural azole antifungals. Disk diffusion assays were performed to evaluate the ability of C. neoformans to develop resistance to these compounds, focusing …

The Development Of Tools For The Production Of Fusarium Oxysporum Race 4 Resistant Cotton Varieties, Thomas Rylands

All Theses

Fusarium oxysporum race 4 (FOV4) is a fungal pathogen that infects cotton (Gossypium spp.) plants, resulting in fusarium wilt (FW) disease that causes significant wilting, yield loss, and eventually death. Since cotton is such an important crop globally, with tens of millions of bales of fiber produced and sold worldwide each year, it is critical to develop effective strategies to combat this pathogen. This research aims to achieve that goal through two projects: (i) develop an efficient co-culture system to test fungal treatments in vitro, and (ii) develop a computer vision pipeline to more accurately assess plant resistance …

Seagrass Carbon Standing Stock In The Northern Gulf Of Mexico: The Role Of Seagrass Beds In Mississippi As Potential Blue Carbon Sinks, Devan Hildebrand

Master's Theses

Organic carbon stored in marine ecosystems is known as blue carbon. Seagrass beds have the ability to contribute to global blue carbon standing stocks through the uptake and sequestration of carbon from the water column. Through coastal management and conservation best practices, blue carbon habitats have the potential to help mitigate the effects of climate change. Although widely studied, knowledge gaps exist on the sediment carbon standing stock of seagrasses. I aim to better understand how persistent seagrass meadows surrounding barrier islands in the northcentral Gulf of Mexico contribute to long-term blue carbon standing stocks. Subaqueous sediment cores were collected …

Context Dependent Regulation Of Dicer Function During Oocyte-To-Embryo Transition, Nick A. Newkirk

Dissertations and Theses (Open Access)

Over two decades ago, Dicer was discovered as the primary enzyme responsible for the generation of small RNAs known as the microRNAs (miRNAs) and short interfering RNAs (siRNAs). Spatiotemporal regulation of Dicer-dependent small-RNA biogenesis affects many aspects of biology, including aging, disease progression, fertility, and cancer. Interestingly, a distinct population of Dicer-dependent small RNAs, known as the endogenous siRNAs (endo-siRNAs), are uniquely abundant in the germ cells and gametes, and are critical for normal fertility. Despite their function regulating new life, the mechanisms controlling their biogenesis and subsequent germ cell functions remain poorly defined. Work to parse these mechanisms in …

Capsinoids, Non-Pungent Trpv1 Agonists, Induce A Mild Hypothermia That Provides Short- And Long-Term Neuroprotection After Ischemic Stroke, Alexander Andersohn, Alexander Andersohn

Dissertations and Theses (Open Access)

Therapeutic hypothermia (TH) has demonstrated neuroprotection in instances of cardiac arrest and neonatal hypoxia/ischemia but faces different challenges in application to stroke due to the activation of cold defense mechanisms in conscious patients. This dissertation examines the efficacy and specificity of capsinoids (a purified mixture of capsiate and dihydrocapsiate) to induce a sustained mild hypothermia in conscious mice that is neuroprotective after stroke. Capsinoids function as TRPV1 agonists. However, unlike capsaicin, capsinoids are vulnerable to esterase-mediated breakdown, thus significantly restricting their action to the site of delivery. We showed that capsinoids delivered intraperitoneally (IP) to mice induced a TRPV1-dependent drop …

Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins

Dissertations and Theses (Open Access)

Bacterial cytokinesis requires precise coordination between cytoplasmic and periplasmic components of the divisome to ensure that membrane constriction and chromosome segregation occur in synchrony. The actin-like protein FtsA plays a central role in this process by tethering FtsZ to the membrane and recruiting downstream division proteins. Despite its essential function, the mechanisms by which FtsA coordinates divisome activation and the recruitment of late-acting proteins remain incompletely defined. To address this, the role of FtsA in activating septal synthesis and recruiting the DNA translocase FtsK was examined using mutational analysis in Escherichia coli.

The FtsA^{M96E R153D} variant, which has …

Neural Dynamics Supporting Flexible Cognition, Meredith J. Mccarty

Dissertations and Theses (Open Access)

Flexible cognition enables humans to adapt their behavior to changing circumstances by prioritizing relevant information and filtering out distractions. This process is thought to be supported by the integration of ongoing behavioral goals with incoming sensory information, but how this integration is realized in the human brain remains unclear. It is unknown how high-order sensory cortices can balance specialized processing with flexible integration of changing behavioral goals. In the following investigations, we use intracranial EEG (iEEG) recordings in humans to study the neural dynamics of flexible cognition during high-order sensory processing. First, we validated assumptions about the locality of iEEG …

Orthogonal Comparison Of Nuclear And Mitochondrial Clonal Architectures In Hematologic Malignancies, Nehali Shah

Dissertations and Theses (Open Access)

Acute myeloid leukemia (AML) is a hematologic malignancy characterized by accumulation of mutations that disrupt hematopoietic differentiation and promote clonal expansion. Understanding how these mutations arise and evolve is essential for improving diagnosis, prognosis, and treatment stratification. Current methods are limited by either restricted genomic coverage (targeted panels) or low throughput and high cost (in single-cell whole genome sequencing, scWGS). An emerging alternative is the use of mitochondrial DNA (mtDNA) mutations as clonal markers.

This study aims to determine whether mitochondrial-derived clonal architectures correlate with nuclear-derived clonal architectures in AML, thereby evaluating mtDNA as a scalable orthogonal tool for lineage …

A Mixed Methods Study Of The Weather And Climate Science Curriculum Pilot In South Carolina K-12 Schools, 4-H, And Agricultural Education Programs, Emily B. Hood

All Dissertations

Weather and climate science topics in K-12 education are more important now than ever due to extreme weather and climate events happening worldwide. Most U.S. science teachers included climate science in their courses, but they may not have a sufficient background in the topic which can hinder their efforts, or there is no explicit curriculum for the teacher to utilize. Research indicates that there is a need for innovation in the curriculum to help prepare children to become the next generation of leaders and address complex problems, such as issues related to weather and climate science. There are a few …

Breeding For Disease Resistance In Melon And Watermelon, Venkata Rao Ganaparthi

All Dissertations

Watermelon and cantaloupe are two widely consumed cucurbits, but their acreage in the United States reduced by nearly 50% in last two decades. Fusarium wilt caused by a soil borne pathogen Fusarium oxysporum f. sp. niveum(Fon) race 2 is a major limiting factor in watermelon production as it reduces yields by 80%. Grafting watermelon plants onto the root stock of wild, disease-resistant varieties like ‘Carolina Strongback’ is the best strategy to manage Fon race 2. Though effective, this approach increases production costs.

Part of this dissertation focused on finding more sustainable, cost-effective solutions to control Fusarium wilt …

Mountain Communities During A Time Of War: A Case Study Of The Hutsul Region In The Carpathian Mountains Of Ukraine, Pavlo Rybaruk

Electronic Theses and Dissertations

This thesis investigates the ethno-ecological technologies employed by the Hutsul people within the highland agrarian economy of the Carpathian Mountains. Over centuries, the Hutsuls have developed ethno-ecological technologies that maintain ecological integrity while supporting community livelihoods.

The study addresses three primary research questions: What are the most important ethno-ecological technologies of the Hutsul highland economy? How might these traditional ethno-ecological practices be applied to economic, agricultural, and tourism development in the Hutsul region? How might these traditional ethno-ecological practices be combined with contemporary approaches to encourage sustainable development in the region?

The research explores the devastating impact of the Russian …

Taxonomy Of Endophytic Fungi Associated With Vallisneria Neotropicalis, Md. Arafat Rashid

Graduate Theses and Dissertations (2019 - present)

This study presents the first comprehensive taxonomic and ecological investigation of endophytic fungi (EF) associated with the submerged aquatic macrophyte Vallisneria neotropicalis in the southern United States. Over a 12 month period, from April 2023 to March 2024, leaf samples were collected from two distinct sites in Mobile Bay, Alabama a disturbed, brackish Causeway location and a cleaner, less impacted site at Meaher State Park. Using culture dependent methods, a total of 257 fungal endophytes were isolated from 1,200 leaf segments. All isolates belonged to the phylum Ascomycota, distributed across 3 classes, 6 orders, 10 families, and 19 taxa. The …