Digital Commons Network^™

Elevated Temperatures Disrupt Wolbachia-Induced Feminisation And Reshape Microbial Community Dynamics Across Generations In A Spider Host, Virginija Mackevicius-Dubickaja, Jennifer A. White, Ellen E. Williams, Eyal Klement, Yuval Gottlieb, Matthew R. Doremus

Entomology Faculty Publications

Longitudinal microbial interactions within a host are challenging to study, leading to a focus on constructed microbial communities in vitro settings. Here, we take advantage of a naturally defined microbial community within a spider host to study how elevated temperatures influence microbial dynamics and phenotypes across host generations. The spider Mermessus fradeorum hosts up to five endosymbionts, including a Wolbachia strain, W1, which induces feminisation, causing genetic males to develop as phenotypic females, skewing sex ratios and promoting symbiont spread. Despite this, Wolbachia 1 persists at intermediate frequencies in wild populations. We hypothesised that elevated temperatures might reduce penetration of …

An Iterative & Analytical Hybrid Deep Learning Framework For Enhanced Brain Tumor Detection And Segmentation In Mri Video Scans, Swati V. Sakhare

Mansoura Engineering Journal

The critical need for accurate and robust brain tumor detection in MRI video scans is for early diagnosis and treatment planning operations. However, the existing methods suffer from several limitations, such as not being able to capture spatio-temporal features, handling class imbalances, and generalizing across datasets with diverse characteristics. To address these challenges, I propose a hybrid deep learning framework that integrates multiple advanced techniques to enhance the detection and segmentation of brain tumors. My approach starts by using the 3D CNN-LSTM (ResNet3D-LSTM) to extract spatio-temporal feature, which utilizes ResNet3D for spatial detail extraction and LSTM for temporal coherence across …

Foundations For Trojan-Y Chromosome Development In Sacramento Pikeminnow (Ptychocheilus Grandis): A Novel Strategy For Invasive Species Management In The Eel River, Ca, Raven Mcadams

Cal Poly Humboldt theses and projects

The Sacramento Pikeminnow (Ptychocheilus grandis), an invasive predator in California’s Eel River, contributes to predation pressure on native salmonid populations despite ongoing suppression programs. This study assessed the feasibility of hormonal sex reversal as an initial step toward implementing the Trojan Y Chromosome (TYC) strategy as a novel tool for managing invasive Sacramento Pikeminnow populations in the Eel River, CA. Objectives were to 1) determine the size when gonadal differentiation occurs 2) identify an effective estradiol dosage for producing phenotypic XY females, and 3) identify sex-reversed fish using a sex-specific genetic marker.  Histological examination of juvenile fish was …

Exploring Students' Perceptions Of Active Learning Practices And Their Role In Critical Thinking Development In Undergraduate Biology Education, Kwame Kyeremah

All Master's Theses

As the global landscape becomes increasingly complex and undergoes more rapid changes than ever before, critical thinking has become an indispensable skill for addressing challenges in society, science, and the workplace. Despite its widespread adoption in undergraduate STEM education, active learning remains poorly understood from the student's perspective. This study investigated how undergraduate biology students perceived active learning in a ten-week biostatistics course (BIOL 213) at Central Washington University, and whether those perceptions aligned with critical thinking gains measured by the California Critical Thinking Skills Test with Numeracy (CCTST-N). A convergent mixed-methods design integrated student perception and mindset survey data …

Ultrasound-Derived Liver Composition And Metabolic Flexibility Under Exercise Stress, Rhodes Van Houten

All Master's Theses

Metabolic Associated Fatty Liver Disease (MAFLD) is characterized by abnormal liver fat accumulation in conjunction with metabolic syndromes. MAFLD affects 33% of individuals worldwide, though little is known about how increased liver fat impacts physiological responses to exercise. This study aimed to analyze the relationship between liver composition and metabolic flexibility during a graded exercise test.  Body composition was analyzed via dual-energy X-ray absorptiometry. Liver ultrasound was evaluated for echogenicity, where elevated mean gray volume (MGV) indicates abnormal liver tissue. Rates of fat (FATox) and carbohydrate (CHOox) oxidation (g/min), respiratory exchange ratio (RER), and blood lactate concentrations ([LA^-], …

Noninvasive Monitoring Techniques: The Use Of Scat-Sourced Dna And Camera Traps To Monitor Mammalian Carnivore Species At The Ucf Arboretum, Zachary Montealegre

Honors Undergraduate Theses

The 800-acre University of Central Florida Arboretum Natural Lands functions as a conservation area, recreational space, and research site, making it essential to understand the ecological community it supports. Traditional wildlife monitoring methods such as mark-recapture and blood sampling can be effective but may stress animals or disrupt natural behaviors. To minimize these impacts, this study used non-invasive techniques, specifically scat-based DNA analysis and camera trapping, to assess carnivore species' presence. Two ecologically similar sites within the Arboretum were selected. Suspected carnivore scat was collected twice weekly over two months using both structured and opportunistic sampling. DNA was extracted, amplified, …

Combining Haptic Feedback With Electromyography To Study Knee Injury In Stationary Cycling, Christopher Kirby

Honors Theses

Patellofemoral Pain Syndrome (PFPS) is the most prevalent overuse injury in cycling, often linked to altered neuromuscular activation patterns of the quadriceps and hamstrings. While sensory feedback has successfully modified cyclists posture, there is a critical lack of evidence-based interventions targeting the underlying muscle activation imbalances associated with chronic knee pain. This study aimed to develop and validate a novel, real-time biofeedback system that utilizes electromyography (EMG) to trigger vibrotactile cues, aiming to shift muscle onset timing earlier in the pedal stroke to mitigate PFPS-related imbalances. A closed-loop system was engineered by integrating a Delsys EMG system with a SageMotion …

A Study Of Adaptive Reuse Of A Decommissioned Golf Course Through A Nature-Based Approach: Woodhaven Golf Course, Fort Wort Texas., Justin B. Watson Mr.

Landscape Architecture Masters & Design Theses

Across the nation, golf courses are shutting down due to a lack of demand. Golf course developments began in the United States in the early 1950s During that era, demand for golf courses and housing was high. As golf’s popularity grew, developers saw golf courses in developments as high value-add amenities (Grooms, 2010). During the late 1990s and early 2000s, developers across the country raced to construct as many golf course communities as they could even as the number of golfers leveled out (Downey,2011).

Decommissioned golf courses across Texas are being underutilized as potential amenities for both the local community …

The Evolution Of California Wildfire Governance Through State Bills, Regulations, And Executive Actions, Cassandra Dawn Blevins

History and Political Science | Senior Theses

California faces an escalating wildfire crisis, driven by climate change, drought, and development in fire-prone areas. Despite decades of scientific research on wildfire behavior and risk reduction, catastrophic fires since 2017 raise a critical question: to what extent have California state laws and policies kept up with the intensifying wildfire crisis? Existing scholarship provides extensive insight into wildfire ecology, land-use planning, fuel management, and community resilience, yet it lacks a comprehensive evaluation of whether California’s legislative, regulatory, and executive actions have adapted in a coordinated and evidence-informed manner. In particular, the literature offers limited analysis of how state laws integrate …

Evaluating The Use Of Precision Technology To Understand Differences Between Ketotic And Non-Ketotic Jersey Cows, Emily Kathryn Palermo

Honors Theses and Capstones

No abstract provided.

Tmem116 Crispr-Mediated Knockout Prevents Proper Lens Regeneration In Iberian-Ribbed Newts, Damien T. Mann

Honors Theses and Capstones

Eye diseases like cataracts, age-related macular degeneration (AMD), and glaucoma are widespread and detrimental conditions affecting many people across the globe. While there are reliable and successful treatments for those with cataracts that can reverse vision impediments, others like glaucoma and AMD have limited treatment options. Additionally, eye damage resulting from these conditions is commonly irreversible, establishing a need for new treatment options. The Iberian-ribbed newt, Pleurodeles waltl, is a salamander species primarily studied for their amazing regenerative capabilities. Particularly, these newts are capable of de novo lens regeneration after a complete resection, providing a unique opportunity to study regeneration …

Anuran Occupancy Of Created Wetlands Within Reforested Legacy Surface Mines In Kentucky And West Virginia, Jillian C. Newman, Steve Price, Jacquelyn C. Guzy, Joshua Castle, John J. Cox, Jeffery L. Larkin, Christopher D. Barton

Forestry and Natural Resources Faculty Publications

Restoration of habitat is an important component of wildlife management. Surface coal mining has altered forest cover in the Central Appalachians, and most reclamation activities on previously mined lands result in non-native grasslands or shrub lands. The Forestry Reclamation Approach (FRA), which emphasizes non-native vegetation removal, soil decompaction, planting of native trees and shrubs, and, in some cases, wetland creation represents an alternative to grassland-based reclamation that may promote forest-associated wildlife on legacy surface mines. We used automated recording units to evaluate the response of anurans to created wetlands and the FRA in Kentucky and West Virginia, USA. We used …

Microglial Apoe Across Alzheimer’S Disease-Relevant Pathologies, Georgia Nolt

Theses and Dissertations--Physiology

APOE exists as three common alleles (APOE2, APOE3, APOE4) that encode their corresponding protein isoforms (apoE2, apoE3, apoE4). Despite differing by only one or two single-amino acid substitutions, each APOE allele confers a distinct AD risk profile with APOE4 increasing risk by 28% and APOE2 decreasing risk by 14% compared to the most commonly expressed APOE3 allele. While traditionally considered “neutral,” APOE3 still accounts for ~36% of AD cases while APOE4 contributes ~57%, indicating that the majority of AD cases would not occur in the absence of the APOE3 and APOE4 alleles. Microglia, the resident phagocytic immune cells …

Characterizing Gut Microbial Communities And Symbiont-Mediated Detoxification In Herbivorous Insects, Rachel C. Vargas

Biology Dissertations

Insect gut bacteria are vital contributors to host physiology, yet the broader prevalence and fitness implications of symbiont-mediated detoxification remain poorly understood. This thesis investigates these dynamics across three research chapters. Chapter 1 uses 16S rRNA sequencing to characterize how grasshopper gut microbial communities vary across species, dietary breadths, and gastrointestinal segments. Chapter 2 evaluates the detoxification capacity of over 150 bacterial isolates from diverse herbivores against the plant allelochemicals phenethyl isothiocyanate, rutin, and chlorogenic acid to determine if host dietary specialization predicts microbial metabolic ability. Finally, Chapter 3 employs fitness bioassays to test whether inoculating generalist herbivores with detoxifying …

How Effective Is Online Teaching Of Physiology Modules? Perception Of Rwandan Health Sciences Students, Abdullateef Isiaka Alagbonsi, Mojeed Akorede Gbadamosi, Mathieu Nemerimana, Makinde V. Olubiyi, Olufunke Onaadepo, Akaninyene Ubong Ime, Abdul-Rahuf Aderemi Feyitimi, Ruth Wanjiru Mungai, Florent Rutagarama, Simiat Elias

Biomedical Sciences, East Africa

Background: While blended teaching is approved in Rwandan medical education, the perceived effectiveness of online-only delivery for physiology modules remains unclear. This uncertainty limits its continued use. The present study assessed Rwandan health sciences students’ perceptions of the effectiveness of online teaching for physiology modules.

Methods: A total of 528 students from medicine and surgery (n = 300), pharmacy (n = 99), dentistry (n = 89), and clinical psychology (n = 40) programs at the University of Rwanda completed an online questionnaire. Data were analyzed using multivariable logistic regression and non-parametric tests (Mann-Whitney U, Kruskal-Wallis) with corresponding effect …

Comparison Of Calcified Structures To Estimate Ages Of Northern Pike Esox Lucius In Various Lakes, Kaed Rauk

Journal of Earth and Life Science

Northern pike Esox lucius are a dominant predatory fish in many aquatic systems. They can have significant impacts on the ecosystems they inhabit and are also a popular game fish among anglers. However, northern pike are a difficult species to age accurately. This study aimed to compare calcified aging structures to identify trends and determine which structure provides the most accurate age estimates. A total of 119 northern pike were sampled, and multiple structures from each fish were independently aged by two readers. The coefficient of variation (CV) was calculated for each structure to assess precision. Sectioned sagittal otoliths exhibited …

Indigenous Land Seizure & Environmental Injustice: Impacts Of Settler Colonialism, Lydia Moorman

Journal of Earth and Life Science

The fields of conservation and environmentalism prioritize ecosystem health, yet both emerged from western frameworks that displaced and criminalized Indigenous land stewardship. The idealization of “pristine wilderness” narratives served to justify the removal of Indigenous peoples from their homelands and erased millennia of sustainable land stewardship that had maintained ecological balance long before western conservation existed. These narratives not only misrepresented Indigenous relationship with land but also constructed a false dichotomy between humans and nature that positioned Indigenous presence as a threat rather than a source of ecological knowledge. Simultaneously, anthropogenic expansion; such as pipelines, mining, railroads, and other extractive …

Understanding Motor Competence In Autism And Developmental Coordination Disorder: From Everyday Performance To Underlying Postural Control Mechanisms, Crystal E. Alvarez

Kinesiology Dissertations

Motor competence plays a foundational role in children’s participation, physical activity, adaptive behavior, and overall quality of life. Although motor difficulties are highly prevalent in autism spectrum disorder (autism) and developmental coordination disorder (DCD), these challenges are frequently under-identified and insufficiently characterized, particularly in autistic populations. Existing research demonstrates substantial overlap in motor challenges across autism and DCD; however, relatively little work has integrated caregiver perceptions, standardized motor assessment, and mechanistic postural control measures within the same investigation. Therefore, the purpose of this dissertation was to examine motor competence and postural control in autistic children, children with DCD, and neurotypical …

Small Mammal Communities Within And Land Coverage Around Southwestern Missouri Prairies, Kaitlyn J. Bebensee

Graduate Theses/Dissertations

Land alterations can reduce the amount of suitable habitat necessary for persistence of an associated species. Within such constrained habitats, limited resources may elicit competition, which could cause shifts in abundance and occurrence of species. Missouri prairies have been declining in size, making it imperative to collect reference data on mammal populations within and land use types around remnants. For this study, I trapped small mammals in Southwestern Missouri prairies because they can be indicators of environmental health and can vary in abundance between prairies. I focus on the diet of two dominant small mammals, Peromyscus maniculatus and Microtus ochrogaster …

Extending The Fruiting Season With Container Grown Primocane-Fruiting Blackberries Grown In A High-Tunnel, Edwin James Tucker Iii

Graduate Theses/Dissertations

Blackberry production has become an important part of many small fruit orchard operations because they’re easy to grow, produce a large crop, and sell well at market. The current blackberry season in Missouri extends from mid-June to mid-August, depending on the cultivar chosen. This fruiting season is common for floricane-fruiting blackberry cultivars (cultivars that fruit on one-year old canes). Primocane-fruiting blackberry, or blackberries that fruit on current season growth, have the potential to further extend the harvest season of blackberry into September and October especially if grown in a high-tunnel. This study had two objectives. The first objective was to …

Perfluorooctane Sulfonate (Pfos)-Induced Disruption Of Mitochondrial Activity And Cell Adhesion In Liver Cells, Phuong Dam Nam Tran

Graduate Theses/Dissertations

Perfluorooctane sulfonate (PFOS) is a persistent environmental pollutant associated with potential hepatoxic effects and other health risks. Despite its widespread distribution, the mechanisms underlying its toxicities remain to be fully understood. To investigate PFOS toxicology, my study utilized HepG2 and THLE-2 human hepatic cell models to replicate conditions reflecting PFOS accumulation in the liver. Cell viability, cell stress, and cell death assays were conducted to assess the toxicological influence of the chemical on both cell lines. Total RNA extraction was performed, followed by cDNA sequencing, and RT-qPCR. The XTT viability assay revealed a dose-dependent decrease in number of viable cells …

Exploring Phosphoregulation Of Myo3a Using Quantitative Fluorescence Image Analysis In Cos7 Cells, Vu Hao Minh Nguyen Phan

Honors Theses

Myosin 3A (MYO3A) is an unconventional myosin involved in the formation and maintenance of hair-cell stereocilia of the sensory epithelia in the inner ear. The kinase domain has been implicated in phosphoregulation of MYO3A activity through intermolecular autophosphorylation. Previous studies using mass spectrometry identified two potential phosphorylation sites in the motor domain. To investigate the regulatory roles of these sites, we generated glutamic acid point mutations in our mchr-MYO3AΔK construct to mimic phosphorylation and assayed the constructs for their ability to tip-localize and influence filopodial density via transfection into COS7 cells. The phosphomimic constructs were less able to generate filopodia …

Sae For All: Curriculum Guide, Emily Carpenter

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

Supervised Agricultural Experience (SAE) is a critical component of agricultural education, designed to provide students with hands-on learning opportunities that extend beyond the classroom. The SAE for All model ensures that every student participates in meaningful experiential learning tailored to their interests and career goals. By integrating SAE into the curriculum, students develop technical skills, career readiness competencies, and an understanding of real-world agricultural applications. This guide includes a teaching plan and a comprehensive set of assignments.

Eeg And Imu Gait Signal Processing: A Comparative Assessment Of The "Reza" Exponential Filter And Classic Filters, Reza Pousti, Daniel M. Russell, Derek C. Monroe, Christopher K. Rhea

Rehabilitation Sciences Faculty Publications

Noise degrades both EEG and gait signals, and classical IIR filters (Butterworth, Chebyshev, elliptic) involve trade-offs between passband flatness, ripple, and roll-off. This study compared a novel exponential "Reza" filter with these designs for neural and locomotor data. We analyzed an open-source mobile brain-body imaging dataset with EEG and gait data from 49 healthy adults (EEG: 256-channel, 512 Hz; IMUs: six APDM Opals, 128 Hz). EEG channels were grand-averaged and band-pass filtered at 0.5-50 Hz, while IMU axes were averaged and band-pass filtered at 0.5-5 Hz. The outcomes were signal-to-noise ratio SNR (dB) and band-integrated Welch PSD (EEG:0.5-50 Hz; IMU:0.5-5 …

Balancing Inquiry Based Learning And Direct Instruction, Sharon Liu , '26

Senior Theses, Projects, and Awards

No abstract provided.

Spiders In The World Of Pesticides: A Review, Jackson L. Mendillo

Williams Honors College, Honors Research Projects

Pesticides and other agrochemicals have varying effects on spiders depending on their mechanism of action and dosage, ranging from behavioral changes and ataxia, to reproductive changes and physiological stress, to lethal non-target effects. Additionally, peptide Hv1a has promising effects as a more narrow-spectrum pesticide.

Reconnecting The Body With Nature: Inspired By Advances In Biomimicry, Elyse M. Orosa

Williams Honors College, Honors Research Projects

Reconnecting the Body with Nature: Inspired by Advances in Biomimicry will visually address the symbiotic relationship between nature and humans. The drawing series is inspired by the growing design field of biomimicry and its technological and architectural innovations influenced by nature’s systems and physical adaptations. Just as nature is limited by human misuse, humans are physically and neurologically limited by the state of the environment, and with this, form and function of both nature and humans will be equally represented and explored throughout the body of work presented. Biomimicry can help solve environmental issues such as rising CO2 emissions and …

Manuka Honey Modulates Oxidative Stress In Neuro2-A Cells Through Mitochondrial Regulation, Rachel Abramovici

Honors Undergraduate Theses

Manuka honey is known for its vital usage in wound healing, being an agonist towards fighting infections, and is an acting antioxidant [32]. Its main constituents are flavonoids and phenolic compounds, with methyl syringate (MSYR) being a major active ingredient. The specific compounds responsible for these antioxidant effects, particularly in the CNS, remain unclear. It is hypothesized that pre-treatment of mouse neuroblastoma cells with MSYR followed by hydrogen peroxide exposure will activate the AMPK/SIRT1/PGC-1a pathway, due to MSYR’s phenolic structure, and thus reduce oxidative stress and improve mitochondrial biogenesis. Hydrogen peroxide is a standard reactive oxygen species (ROS) treatment to …

Impact Of Lifestyle Factors On Allergic Rhinitis Symptoms And Quality Of Life Among College Students, Lina Saint-Hilaire

Honors Undergraduate Theses

This study aims to examine the influence of certain lifestyle factors on the presentation of allergic rhinitis symptoms and the quality of life of college students. Factors of interest include residential setting, household cleaning habits, age of symptom onset, and exposure to household pets. Additional analysis includes assessment of the impact of allergic rhinitis symptoms on sleep. Using a cross-sectional survey design, student responses were self-reported through a paper survey distributed during tabling days at the University of Central Florida (UCF) main campus. Eligible participants had to be 18 years or older and current UCF graduate and undergraduate students. Survey …

Multicomponent Deoxyribozyme Probes For Targeted Detection Of Cancer Associated Gene Insertions, Samuel R. Veleke

Honors Undergraduate Theses

Insertions and deletions (indels) in DNA sequences of genes are linked with cancers such as glioblastoma (GB). Thus, indels may be used as potential cancer biomarkers. In the NANOG gene, its pseudogene NANOGP8 has a 22 base pair insertion sequence for GB patients. In this work, the NANOG gene and the insertion sequence were detected in one sample using a dual-fluorescent assay containing two multicomponent split deoxyribozyme (Dz) probe complexes. One complex used TXR fluorescence to detect the gene regardless of the insertion’s presence. The other complex used fluorescein (FAM) fluorophore to detect the insertion. The probes were able to …