Digital Commons Network^™

Genetic Connectivity Of Trypanosomes Between Tsetse-Infested And Tsetse-Free Areas Of Kenya, Naomi N. Kimenyi, Kelvin M. Kimenyi, Nelson O. Amugune, Merid N. Getahun

All Peer-Reviewed Publications

The prevalence rates of trypanosomes, including those that require cyclical transmission by tsetse flies, are widely distributed in Africa. Trypanosoma brucei and Trypanosoma congolense are actively maintained in regions where there are no tsetse flies although at low frequencies. Whether this could be due to an independent evolutionary origin or multiple introduction of trypanosomes due to continuous movement of livestock between tsetse-free and -infested areas is not known. Thus, the aim of the study was to carry out microsatellite genotyping to explore intra-specific genetic diversity between T. (Trypanozoon), T. congolense and Trypanosoma vivax from the two regions: tsetse infested and …

Centering Community Voices In Mining Governance, Anna Erwin, Zhao Ma, Emma Patricia Salas O’Brien, Jonathan Bauchet, Nelly Ramírez Calderón, Eliseo Zeballos Zeballos, Glenn Roberto Arce Larrea, Ruxandra Popovici, Laura Zanotti, Chelsea A. Silva

Department of Agricultural Economics Faculty Publications

Peru has shifted away from centralized mining management to governance among government, companies, and communities. Various mechanisms facilitate community participation, including the mining canon, dialogues, and corporate social responsibility programs. Even with these laws and mechanisms, mining pollution and conflicts continue. In this study, we ask: how do communities perceive and participate in mining governance? And what are some alternative ways, driven by community priorities, to address governance in mining contexts? We collected 53 semi-structured with agricultural actors in two Peruvian districts with mining activity and analyzed those perspectives through the lens of community-centered governance. Our analyses revealed how centering …

Rethinking Public Health Risk Mitigation Strategies: Recognizing The Biosocial Nature Of Covid-19, Grace Savard

CSB and SJU Distinguished Thesis

The SARS-CoV-2 virus has rippled throughout every aspect of society and influenced how we live, breathe, and interact with one another. The purpose of this thesis is to explore the interdisciplinary biosocial impacts of the SARS-CoV-2 virus and the resulting disease, COVID-19. In order to understand the complexities of caregiving during COVID-19, we conducted 75 semi-structured interviews with 55 healthcare providers across 18 states over a span of two years. This research assesses how COVID-19 risk mitigation strategies were designed with the primary intention to stop the spread of the virus rather than account for the longer-term social and biological …

Divergent Water Requirements Partition Exposure Risk To Parasites In Wild Equids, Kaia J. Tombak, Laurel A. Easterling, Lindsay Martinez, Monica S. Seng, Liana F. Wait, Daniel I. Rubenstein

Publications and Research

For grazing herbivores, dung density in feeding areas is an important determinant of exposure risk to fecal- orally transmitted parasites. When host species share the same parasite species, a nonrandom distribution of their cumulative dung density and/or nonrandom ranging and feeding behavior may skew exposure risk and the relative selection pressure parasites impose on each host. The arid-adapted Grevy's zebra ( Equus grevyi ) can range more widely than the water-dependent plains zebra ( Equus quagga ), with which it shares the same species of gastrointestinal nematodes. We studied how the spatial distribution of zebra dung relates to ranging and …

Ability Of Lifeguards To Detect Submerged Manikins In Public Swimming Pool Environments, Élie Vignac, Pascal Lebihain, Brice Guignard, Natacha Heutte, Loic Le Minor, Bastien Soulé

International Journal of Aquatic Research and Education

To prevent drownings in public swimming pools (PSP), French legislation requires constant surveillance by state-certified lifeguards. While previous research showed that surveillance was not always effective, this article focuses on efficiency of surveillance when it is effective. We evaluated the ability of 4 volunteer professional lifeguards to detect a submerged manikin under controlled conditions. One hundred and eight (108) tests were carried out in 2 PSP. Four variables were controlled (i.e., distance, depth, surveillance station, attendance). Our results showed that rapid drowning detection was not exclusively linked to the individual detection capabilities of a lifeguard, but rather it emerged from …

Grasses As Biofactories: Scoping Out The Opportunities, Nick Roberts, K. Richardson, G. Bryan, C. Voisey, W. Mcnabb, T. Conner, M. Christey, R. Johnson

IGC Proceedings (1977-2023)

Key points

1. Plant biopharming is set to dominate commercial recombinant protein expression for specific proteins.

2. The choice of plant species depends on a multitude of factors and is determined on a caseby- case basis.

3. As a leaf based expression system grasses would have to compete predominantly with tobacco and alfalfa.

4. The grass-endophyte symbiosis offers a number of unique possibilities for biopharming.

Understanding The Microbial Biogeography Of Ancient Human Dentitions To Guide Study Design And Interpretation, Zandra Fagernäs, Domingo C. Salazar-Garcia, Maria Hadar Uriarte, Azucena Avilés Fernández, Amanda G. Henry, Joaquín Lomba Maurandi, Andrew T. Ozga, Irina M. Velsko, Christina Warinner

Biology Faculty Articles

The oral cavity is a heterogeneous environment, varying in factors such as pH, oxygen levels, and salivary flow. These factors affect the microbial community composition and distribution of species in dental plaque, but it is not known how well these patterns are reflected in archaeological dental calculus. In most archaeological studies, a single sample of dental calculus is studied per individual and is assumed to represent the entire oral cavity. However, it is not known if this sampling strategy introduces biases into studies of the ancient oral microbiome. Here, we present the results of a shotgun metagenomic study of a …

How Experts Have Dominated The Neuroscience Narrative In Criminal Cases For Twelve Decades: A Warning For The Future, Deborah W. Denno

William & Mary Law Review

Phineas Gage, the man who survived impalement by a rod through his head in 1848, is considered “one of the great medical curiosities of all time.” While expert accounts of Gage's post-accident personality changes are often wildly damning and distorted, recent research shows that Gage mostly thrived, despite his trauma. Studying past cases such as Gage’s helps us imagine—and prepare for—a future of law and neuroscience in which scientific debates over the brain’s functions remain fiery, and experts divisively control how we characterize brain-injured defendants.

This Article examines how experts have long dominated the neuroscience narrative in U.S. criminal cases, …

Investigating The Presence Of Cladocera Species Within The Wetlands Of Uae, Shamma Eisa Salem Shahdad Alneyadi

Theses

The wetlands ecosystems found in the Arabian Peninsula have been poorly investigated. Moreover, the available information about the presence of freshwater communities have been rarely documented. Although there are scattered records about the presence of freshwater Cladocera organisms within the Arabian Gulf countries; few of them have received proper taxonomic identification. Cladocera populations in freshwater bodies are always characterizing its water quality and other living invertebrates’ community. United Arab Emirates wetlands are among the ecosystems belonging to Ramsar convention for its conservation. The presence of both temporary and permanent freshwater area has been developed after the great interest to collect …

Consumption Of Sugar-Sweetened Beverages And Its Potential Health Implications In Indonesia, Ratu Ayu D. Sartika, Atmarita Atmarita, M. I Zulkarnain Duki, Saptawati Bardosono, Lindawati Wibowo, Widjaja Lukito

Kesmas

The broad availability of sugar-sweetened beverages (SSBs) in the Indonesian market is increasing consumption. It, combined with escalating incidence and prevalence of diabetes and related non-communicable diseases (NCDs), and the ongoing debate on policies, has called for a comprehensive review as described in this study. Data was compiled from various sources but mainly gathered from the reported or published documents because of no direct access to the necessary data set. The lack of studies that assessed the direct relationship between SSB consumption and health outcomes in the Indonesian context also became a strong reason for the preparation of this review …

Pastoralist Conservation: Local Ecological Knowledge And Collective Action For Grassland Conservation In Sierra De Segura (Spain), P. Sanosa-Cols, F. Ravera, F. Dominguez, M. Ventura

IGC Proceedings (1977-2023)

Even if Pastoralism is not practised anymore by a majority of families in the Sierra de Segura (south-eastern Andalusia, Spain), it is still considered a paradigmatic way of life and management of its highlands and a key cultural heritage deeply rooted in local identity. In this article we explore two pastoral communities of this region, Santiago and Pontones, with their body of situated knowledge of the territory and natural resources, which helps pastoralists to organize their livelihoods, while requiring collective organization to manage access to grasslands. Through an ethnographic research based on participant observation and in-depth interviews, we explore the …

Chronic Effects Of Heavy Load Activity Performed Before Resistance Training Sessions On The Physical Performance Of Youth Soccer Players, Guilherme Borsetti Businari, Julio Benvenutti Bueno De Camargo, Paulo Henrique Barbosa, Felipe Alves Brigatto, Marcelo Saldanha Aoki, Tiago Volpi Braz, Charles Ricardo Lopes

International Journal of Exercise Science

International Journal of Exercise Science 14(6): 1421-1434, 2021. The adoption of resistance training (RT) programs has been shown to positively influence sports performance-related parameters. However, the chronic effects of maximal strength protocols on the performance of soccer players are not completely investigated. The aim of this study was to evaluate the effects of performing two repetition maximum (2RM) before a resistance training session on the physical performance of youth soccer players. Seventeen players (under-20 category) were allocated in one of the following groups: 2RM + resistance training group (2RM + RT, n = 8) and resistance training group (RTG, n …

Developing A Glossary Of People-Focused Terms Related To Rangelands And Grasslands, Dana Kelly, Katherine I. Johnsen, Chris Magero, Tungalag Ulambayer, Ann Waters-Bayer

IGC Proceedings (1977-2023)

Excellent glossaries on rangelands and grasslands have been developed by the Society for Range Management (SRM), the International Grassland Congress (IGC) and the International Rangeland Congress (IRC). However, these are largely confined to biophysical and technical terminology and contain very few concepts referring to social, institutional and policy aspects of using rangelands and grasslands. After the 10^th IRC in Saskatoon, Canada, in 2016, an informal group started to develop a glossary of such “people-focused” terms. The short and non-academic definitions are meant to improve communication and understanding by users/practitioners in rangeland and grassland management, policymakers, teachers, students, journalists and …

Comparative Analysis Of Cbrm Cases In Kenya, Ethiopia And Tunisia, I. N. Nganga, L. W. Robinson, B. Eba, F. Flintan, E. M. Ontiri, M. Sghaier, N. H. Abdu, S. S. Moiko

IGC Proceedings (1977-2023)

In various countries, development and conservation organizations and national policymakers have been experimenting with ways of applying the community-based natural resource management approach to the unique social and biophysical characteristics of pastoralist rangeland settings, with mixed results. We carried out comparative case study research on community-based rangeland management (CBRM) in a variety of settings in Ethiopia, Kenya and Tunisia with the objective of identifying what kinds of strategies and methods work in which social and ecological contexts. We used an “options by context” approach guided by a research protocol that includes key variables and descriptors for characterizing the implementing organization’s …

Rare Coding Variants In Rcn3 Are Associated With Blood Pressure, Karen Y. He, Tanika N. Kelly, Heming Wang, Jingjing Liang, Luke Zhu, Brian E. Cade, Themistocles L. Assimes, Lewis C. Becker, Amber L. Beitelshees, Lawrence F. Bielak, Adam P. Bress, Jennifer A. Brody, Yen-Pei Christy Chang, Yi-Cheng Chang, Paul S. De Vries, Ravindranath Duggirala, Ervin R. Fox, Nora Franceschini, Anna L. Furniss, Yan Gao, Donna K. Arnett

Epidemiology and Environmental Health Faculty Publications

BACKGROUND: While large genome-wide association studies have identified nearly one thousand loci associated with variation in blood pressure, rare variant identification is still a challenge. In family-based cohorts, genome-wide linkage scans have been successful in identifying rare genetic variants for blood pressure. This study aims to identify low frequency and rare genetic variants within previously reported linkage regions on chromosomes 1 and 19 in African American families from the Trans-Omics for Precision Medicine (TOPMed) program. Genetic association analyses weighted by linkage evidence were completed with whole genome sequencing data within and across TOPMed ancestral groups consisting of 60,388 individuals of …

Attitudes Toward The Environment: How Do The Attitudes Of Conventional, No-Till, And Organic Farmers Compare?, George A. Youngs, David L. Watt

Great Plains Sociologist

One of Che key dynamics in today's increased interest in alternative fanning is concern for the environment (Beus and Dunlap, 1990). Many advocates of alternative farming argue that conventional farming harms the environment and may even destroy the future of agriculture. The implicit, and often explicit, notion associated with this view is that conventional farmers are less concerned about the environment than are alternative farmers. The present study will test this notion by comparing the attitudes toward the environment of conventional farmers with two types of alternative farmers, organic and no-till. By definition, conventional farmers are those farmers who practice …

Tracing The Path Of Technology Diffusion: The Case Of Nturumenti, Narok, Kenya, Paul N. Katiku, R. Kimitei, S. Amboga, G. A. Keya, B. Korir, J. Nginyi

IGC Proceedings (1977-2023)

An array of technologies; growing improved wheat varieties, bulking and collective marketing of wheat grains, sheep breed improvement, ram sharing, feedlot sheep fattening for market, strategic sheep deworming, pasture establishment and conservation practices; were introduced to members of a community based organization (CBO) in Nturumenti, anti-female genital mutilation and anti-poverty organization (AFAPO) in 2014. The CBO members, composed of youthful 19 men and 7 females, were intensively exposed and involved in the new skills both theoretically and practically in the farmers’ field school model. Even though the 8 farming technology options were suitable for the study environment, their adoption and …

Effect Of Water Depth On Heart Rate And Core Temperature During Underwater Treadmill Walking, Carrie E. Bajenski, Brianna R. Brandon, Cailey A. Curry, Leslie Fajardo, Ryan T. Conners

International Journal of Aquatic Research and Education

Exercising using an underwater treadmill (UTM) has become a popular modality; however, few studies have focused on the physiological demands of UTM walking at varying water depths. Thus, the objective of this study was to investigate changes in heart rate (HR) and core temperature (CT) values in college-aged males and females while exercising at different water immersion depths using an UTM. Twenty participants (age = 21.50 ± 2.19 years; height = 169.04 ± 10.85cm; weight = 75.56 ± 22.28kg) walked at water depths of 10cm below the xiphoid process and at the level of the superior iliac crest (I.C.). Each …

Linking Ground, Space And Knowledge: The Role Of Weather Forecasting In Pastoralists' Decision-Making, E. L. Mulder, V. C. Wright, S. C. Steele-Dunne, P. H. A. J. M. Van Gelder, H. C. Winsemius

IGC Proceedings (1977-2023)

Changing weather patterns and decreasing land availability continue to challenge the livelihood of the pastoralists in northern Tanzania. The increasing variability of expected rains has complicated livestock management, often jeopardizing household resilience. Drought Early Warning Systems are being set up to contribute to decision-making processes at national and international levels. Nevertheless, due to the large spatial- and temporal resolution of these systems and their high uncertainties, these systems have limited value at a pastoral household level.

Therefore, this paper explores what type of weather and climate information is deemed valuable for pastoral households in Longido District, Tanzania. It is based …

Climate Change Awareness In The United Arab Emirates, Mariam Matar Abdulla Mohammed Almheiri

Theses

Climate change has focused public attention in the United Arab Emirates (UAE) on the role of society in environmental protection. The objective of this study was to determine the level of public awareness of climate change in the seven emirates of the UAE. The study also aimed to determine the spatial and geographical distribution and gender differences, as well as to investigate the possible influence of human activities on climate change impacts. In a cross-sectional survey, the researcher used a questionnaire to collect data from a sample of 4000 participants to answer the research questions. The drop-off pick-up (DOPU) method …

Ethical, Legal, And Social Issues In The Earth Biogenome Project, Jacob S. Sherkow, Katharine B. Barker, Irus Braverman, Robert Cook-Deegan, Richard Durbin, Carla L. Easter, Melissa M. Goldstein, Maui Hudson, W. John Kress, Harris A. Lewin, Debra J. H. Mathews, Catherine Mccarthy, Ann M. Mccartney, Manuela Da Silva, Andrew W. Torrance, Henry T. Greely

Journal Articles

The Earth BioGenome Project (EBP) is an audacious endeavor to obtain whole-genome sequences of representatives from all eukaryotic species on Earth. In addition to the project’s technical and organizational challenges, it also faces complicated ethical, legal, and social issues. This paper, from members of the EBP’s Ethical, Legal, and Social Issues (ELSI) Committee, catalogs these ELSI concerns arising from EBP. These include legal issues, such as sample collection and permitting; the applicability of international treaties, such as the Convention on Biological Diversity and the Nagoya Protocol; intellectual property; sample accessioning; and biosecurity and ethical issues, such as sampling from the …

A Call For The Library Community To Deploy Best Practices Toward A Database For Biocultural Knowledge Relating To Climate Change, Martha B. Lerski

Publications and Research

Abstract

Purpose – In this paper, a call to the library and information science community to support documentation and conservation of cultural and biocultural heritage has been presented.

Design/methodology/approach – Based in existing Literature, this proposal is generative and descriptive— rather than prescriptive—regarding precisely how libraries should collaborate to employ technical and ethical best practices to provide access to vital data, research and cultural narratives relating to climate.

Findings – COVID-19 and climate destruction signal urgent global challenges. Library best practices are positioned to respond to climate change. Literature indicates how libraries preserve, share and cross-link cultural and scientific knowledge. …

Emory-Tibet Science Initiative: Changes In Monastic Science Learning Motivation And Engagement During A Six-Year Curriculum, Kelsey M. Gray, Cindy Achat-Mendes, Ann Cale Kruger, Tashi Lhamo, Rinchen Wangyal, Gelek Gyatso, Carol M. Worthman

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Led by His Holiness the Dalai Lama, the initiative taken by the Tibetan Buddhist monastic community to connect with western science and scientists presents a unique opportunity to understand the motivations and engagement behaviors that contribute to monastic science learning. In this study, we draw on quantitative data from two distinct surveys that track motivations and engagement behaviors related to science education among monastic students. The first survey was administered at one monastic university in 2018, and the second follow-up survey was completed by students at two monastic universities in 2019. These surveys assessed the reception of science education related …

Total Energy Expenditure Is Repeatable In Adults But Not Associated With Short-Term Changes In Body Composition, Rebecca Rimbach, Yosuke Yamada, Hiroyuki Sagayama, Philip N Ainslie, Lene F Anderson, Liam J Anderson, Lenore Arab, Issaad Baddou, Kweku Bedu-Addo, Ellen E Blaak, Stephane Blanc, Alberto G Bonomi, Carlijn V C Bouten, Pascal Bovet, Maciej S Buchowski, Nancy F Butte, Stefan G J A Camps, Graeme L Close, Jamie A Cooper, Sai Krupa Das, Lara R Dugas, Ulf Ekelund, Sonja Entringer, Terrence Forrester, Barry W Fudge, Annelies H Goris, Michael Gurven, Catherine Hambly, Asmaa El Hamdouchi, Marije B Hoos, Sumei Hu, Noorjehan Joonas, Annemiek M Joosen, Peter Katzmarzyk, Kitty P Kempen, Misaka Kimura, William E Kraus, Robert F Kushner, Estelle V Lambert, William R Leonard, Nader Lessan, Corby K Martin, Anine C Medin, Erwin P Meijer, James C Morehen, James P Morton, Marian L Neuhouser, Theresa A Nicklas, Robert M Ojiambo, Kirsi H Pietiläinen, Yannis P Pitsiladis, Jacob Plange-Rhule, Guy Plasqui, Ross L Prentice, Roberto A Rabinovich, Susan B Racette, David A Raichlen, Eric Ravussin, Rebecca M Reynolds, Susan B Roberts, Albertine J Schuit, Anders M Sjödin, Eric Stice, Samuel S Urlacher, Giulio Valenti, Ludo M Van Etten, Edgar A Van Mil, Jonathan C K Wells, George Wilson, Brian M Wood, Jack Yanovski, Tsukasa Yoshida, Xueying Zhang, Alexia J Murphy-Alford, Cornelia U Loechl, Amy H Luke, Jennifer Rood, Dale A Schoeller, Klaas R Westerterp, William W Wong, John R Speakman, Herman Pontzer

Children’s Nutrition Research Center Staff Publications

Low total energy expenditure (TEE, MJ/d) has been a hypothesized risk factor for weight gain, but repeatability of TEE, a critical variable in longitudinal studies of energy balance, is understudied. We examine repeated doubly labeled water (DLW) measurements of TEE in 348 adults and 47 children from the IAEA DLW Database (mean ± SD time interval: 1.9 ± 2.9 y) to assess repeatability of TEE, and to examine if TEE adjusted for age, sex, fat-free mass, and fat mass is associated with changes in weight or body composition. Here, we report that repeatability of TEE is high for adults, but …

An Integrated Framework To Study Ecological Tipping Points In Social-Ecological Systems, F. A. Männer, L.-M. Schwarz, D. A. Menestrey-Schwieger, V. Amputu, M. C. Bilton, K. Brinkmann, G. Dressler, N. Hamunyela, H. Heita, S. Heshmati, S. Liehr, M. Mbidzo, F. Munyebvu-Chambara, W. C. Nesongano, M. Rauchecker, A. Sandhage-Hofmann, K. Tielbörger, K. Zimmer, A. Linstädter

IGC Proceedings (1977-2023)

Sudden regime shifts or tipping points pose a major threat to various ecosystems and people's livelihoods worldwide. However, tipping points are still hard to predict and often occur without warning. To avoid dramatic social-ecological consequences, it is crucial to understand tipping point behaviour and to identify early warning indicators. Previous studies have hardly implemented an integrated social-ecological approach, which has led to a fragmented understanding and oversimplification of tipping point phenomena. Against this background, we present a systemic research framework that harmonizes ecological and social perspectives to gain a mechanistic understanding of tipping point behaviour. We utilize a social-ecological systems …

Invited Perspective - Nutritional Needs And Implications For Children In Subsistence Marketplaces, Nagendra Rangavajla

Subsistence Marketplaces

Today, while the number of stunted children is decreasing in all geographies, the progress is not consistent. Moreover, there is an increasing prevalence of overweight and obesity among children and adolescent. Globally, about half of all children under five do not receive essential nutrients, often unnoticed until too late. On the other end of the spectrum, the incidence of overweight and obesity in 5-19 year old has increased from 4% in 1975 to 18% in 2016 ¹. These trends reflect a ‘triple burden of malnutrition’, a burden that impacts the survival, growth, and development of children, and in turn, …

Unraveling Ancient Lake Conditions Through Eunotia Identification, Erik Duffy Oddsund

Botany Honors Papers

No abstract provided.

Sure 2022 Undergraduate Science Conference Booklet, Sure Network

Group Reports

The SURE 2022 Conference was the fifth series of Science Undergraduate Research Experience (SURE) Conferences. The conference took place on Friday, 14th October, 2022, co-hosted by SETU – Carlow campus and TU Dublin as a live Face2Face event running simultaneously in both venues on the same day. Students from throughout Ireland completing their Final Year Project in a science discipline in 2021-22 presented their undergraduate research work to this conference.

The aims of each of the SURE conferences are to:

• Provide current students with an opportunity to gain an understanding of the work which has been undertaken by recent graduates, …

Evolving Evolutionary Psychology, Darcia Narvaez, David S. Moore, David C. Witherington, Timothy I. Vandiver, Robert Lickliter

Pitzer Faculty Publications and Research

Which evolutionary theory can best benefit psychological theory, research, and application? The most well-known school of evolutionary psychology has a narrow conceptual perspective (a.k.a., “Narrow Evolutionary Psychology” or NEP). Proponents of NEP have long argued that their brand of evolutionary psychology represents a full-fledged scientific revolution, with Buss (2020) recently likening NEP’s scientific impact to that of a Copernican or Darwinian paradigm shift. However, NEP stands on two traditions that are now the subjects of serious debate and revision: the neo-Darwinian adaptationist framework within evolutionary biology, and the computationalist “mind-as-computer” framework within cognitive science. Although NEP calls itself revolutionary, the …

Genetic Diversity And Population Structure Of Local Sudanese Sheep Populations Revealed By Whole-Genome Sequencing, A Amane, G Belay, Abdulfatai Tijjani, T Dessie, H H. Musa, O Hanotte

Faculty Research 2022

Local Sudanese sheep populations inhabiting diverse environmental conditions and holding opposing morphologies provide opportunities for molecular-genetic research. Characterizing their genome is crucial for sustainable breeding improvement and targeting favorable genes in breeding programs. However, the genome of these sheep populations, which comprises several subtypes, remains uncharacterized using whole-genome sequence data. This study aimed to elucidate genome-wide genetic diversity and population structure of 11 local Sudanese sheep populations, namely, Hammari, Kabbashi, Meidobe, Ashgar, Dubasi, Watish, Bega, Naili, Fulani, Zagawi, and Garag. Ninety whole blood samples were collected, and we extracted DNA using a Qiagen DNeasy® extraction kit. We used the Illumina …