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Jnk Signaling Impacts Car-T Cell Effector Function, Charles Kuhlmann

ETDs from 2020-2029

The potent cytotoxic activity of T cells serves as a natural defense against cancers. Chimeric antigen receptor (CAR) T cell therapy represents a strategy for harnessing T cells against advanced tumors. CAR-T cell therapy has seen striking success against hematologic malignancies, prompting its application to new diseases. Ovarian cancer is a highly lethal disease characterized by frequent recurrence and aggressive nature, prompting the investigation of CAR-T cell therapy as a salvage regimen. However, like in other solid tumors, the early performance of CAR-T cell therapy in ovarian cancer is poor. While many strategies exist for improving the longevity of CAR-T …

Intrathecal (G4c2)149 Delivery In C9orf72-Deficient Mice Yields Mild Motor Dysfunction And Als/Ftd Pathological Hallmarks, Katelyn Russell, Amelia Shahrabi, Suleyman Akerman, Matthew D. Byrne, Jeffrey Rothstein, Davide Trotti, Brigid Jensen, Aaron Haeusler

Farber Institute for Neuroscience Faculty Papers

A repeat expansion in C9ORF72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), yet existing mouse models incompletely engage spinal regions implicated in disease. Here, an adeno-associated virus encoding (G4C2)149 repeats was delivered via neonatal intrathecal injection, achieving widespread CNS expression with robust spinal cord targeting. This approach was applied to mice with graded loss of endogenous C9orf72 to interrogate both gain- and loss-of-function mechanisms. Longitudinal motor, behavioral, and pathological analyses revealed that repeat expression primarily drives mild, progressive muscle weakness, whereas coordination deficits were largely genotype dependent. Subtle gait abnormalities and hyperactivity …

Multidimensional Scaling (Mds) Analysis Of Fire Blight Resistance In Apple Genotypes Selected From The Eastern Black Sea Region Of Türkiye, Kubi̇lay Kurtuluş Baştaş, Hati̇ce Dumanoğlu, Veli̇ Erdoğan, Ahmet Aygün, Rabi̇a Albayrak Deli̇ali̇oğlu, Sai̇d Efe Dost, Ümi̇t Serdar, Özgün Kalkişim, Sali̇h Maden, Zahi̇de Kocabaş

Turkish Journal of Agriculture and Forestry

Erwinia amylovora is the causal agent of fire blight, which is known as one of the most destructive diseases of apple. The use of fire blight-resistant cultivars/rootstocks is one of the most effective methods available to manage this disease. We previously selected 199 local apple cultivars that showed no clearly visible symptoms of fire blight on the plants from the coastline of the eastern Black Sea Region in Türkiye. The region is characterized by the highest rainfall and humidity levels in the country. In this research, young plants of these genotypes grafted onto MM.106 rootstock were screened for fire blight …

Effects Of Fenugreek And Shatavari On Vasomotor And Hormonal Balance In Ovariectomized Rats, Füsun Erten, Beşi̇r Er, Cemal Orhan, Abhijeet A. Morde, Muralidhara Padigaru, Kazi̇m Şahi̇n

Turkish Journal of Agriculture and Forestry

Trigonella foenum-graecum L. (fenugreek, TFG) and Asparagus racemosus Willd. (shatavari, AR) have been extensively used in Ayurvedic, Unani, and traditional folk medicine to support women’s health. TFG seeds are traditionally employed as galactagogues and hormonal modulators, while AR roots are valued as rejuvenating tonics with phytoestrogenic activity, prescribed for menopausal complaints and reproductive well-being. Both plants also exhibit antioxidant and hepatoprotective effects, suggesting their potential as safe, nonhormonal alternatives to conventional hormone replacement therapy. This study investigated the dose-dependent effects of TFG and AR, administered individually or in combination, on vasomotor symptoms, hormonal balance, oxidative stress, and neuroendocrine markers in …

Derived Smoke Solution Mitigates Drought Stress In Forage Pea By Enhancing Antioxidant Defense Mechanisms, Medi̇ne Çopur Doğrusöz

Turkish Journal of Agriculture and Forestry

This study was conducted to enhance plant resilience against drought stress, a major threat to agricultural production, by utilizing an eco-friendly application derived from agricultural waste. We investigated the protective effect of a smoke solution, derived from poppy harvest residues, on the biometric and biochemical parameters of two forage pea (Pisum sativum spp. arvense L.) varieties (GAP Pembesi and Özkaynak). Seeds were primed with 1% and 10% smoke solutions and grown under three distinct conditions: normal irrigation (control), moderate drought, and severe drought. Parameters including biometric traits, chlorophyll pigments, secondary metabolites, and antioxidant enzyme activities were analyzed. The smoke solution …

Processing Mechanism And Efficacy Maintenance Strategy Of Ginseng Active Ingredients Based On Spectroscopy Technology, Yang Hantong, Kong Linghui, Jiang Mengmeng, Wang Yingxiu, Li Xiangguo

Food and Machinery

Ginseng, as a medicinal and edible plant, has ginsenosides as its core active components, which are widely applied due to their physiological functions such as antioxidant and anti-inflammatory activities; however, their structures and activities are easily affected by food processing. During processing steps such as cleaning, steaming/boiling, and fermentation, temperature, pH, and microbial enzyme systems alter the types, contents, and stability of active components through reactions such as hydrolysis and oxidation. The intrinsic linkage mechanism of "component transformation-activity-bioavailability" constitutes a core focus and challenge in current research. This study, based on spectroscopic techniques including high-performance liquid chromatography (HPLC), nuclear magnetic …

Determination Of Eight Fungicide Residues In Eggs By Solid-Phase Extraction Coupled With Liquid Chromatography-Tandem Mass Spectrometry, Liang Jun, Chen Xiong, Wang Yu, Qu Zihao, Zhang Wei, Li Qing

Food and Machinery

［Objective］ To establish an analytical method for the determination of residues of eight fungicides ametoctradin, triadimefon,triadimenol, flutriafol, mandipropamid, penthiopyrad, isopyrazam, and prochloraz ) in eggs by solid -phase extraction (SPE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS ).［Methods］ The samples are ultrasonically extracted with acetonitrile. The extracts are purified using ProElut DPC solid -phase extraction cartridges. The purified eluate is concentrated under reduced pressure and reconstituted with acetonitrile.Chromatographic separation is performed on a Kinetex C18 column (2.6 μm,2.1 mm ×100 mm ) with gradient elution using 0.1% formic acid in water and acetonitrile as the mobile phase. Detection is carried …

Design And Test Analysis Of Automatic Disemboweling Device For Chilled Fresh Duck Carcasses, Wang Yuhao, Bai Zongchun, Zhao Shida, Liu Yunping, Zheng Hao

Food and Machinery

［Objective］ To solve the problems of low automation level and high labor intensity in the disemboweling process of the current meat duck slaughtering and processing technology.［Methods］ With chilled fresh duck carcasses as the research object, an automatic disemboweling device for chilled fresh duck carcasses was created by simulating the existing manual disemboweling method. A special disemboweling tool was designed. Mathematical analysis was used to conduct motion analysis and spatial curve fitting of the disemboweling action of the tool, so as to obtain the characteristic equation and analyze the rationality of the design of the duck carcass disemboweling tool. In addition, …

Effect Of Garlic Paste Addition On Volatile Flavor Substances In Crayfish Tails, Tu Jianqiu, Fu Wenjun, Yang Xiaocong, Jing Lixiang, Zhu Jing, Li Jianfang

Food and Machinery

［Objective］ To investigate the effect of garlic paste addition on volatile flavor substances and color attributes of crayfish tails, and provide a theoretical basis for flavor standardization in industrial production.［Methods］ This paper adopts gas chromatography-ion mobility spectrometry (GC-IMS ) combined with colorimetric analysis to study the characteristics of volatile components and color parameters (L*,a*,b* values ) of crayfish tails under garlic paste additions of 0%,4.4%,8.8%,13.2%,17.6%,and 22.0%. Principal component analysis (PCA ) is applied to analyze flavor differences.［Results］ Colorimetric analysis reveals that garlic paste addition significantly decreases L* from 45.69 to 34.50 and b* from 39.24 to 29.94 (P<0.05), while a* values show a significant difference under the garlic paste addition of more than 13.2% (P<0.05). A total of 53 volatile substances are identified by GC-IMS, and their total content increases gradually with the rising garlic paste addition (highest value in the 22% group at 18 749 and lowest value in the control group at 10 758). Camphene, 2-ethylbutanoic acid, 2-methylbutyl acetate, allyl isothiocyanate, and heptanal are identified as intrinsic flavor markers of crayfish meat. Key contributors to flavor include camphene, 1-heptanol, 3-pentenenitrile, 2,3-pentanedione, diacetyl, and isopropanol. PCA shows that the 13.2%~17.6% group exhibits significantly different flavor characteristics from other groups (PC1 contribution rate of 76%).［Conclusion］ Under the garlic paste addition of 8.8%~13.2%, the optimal balance between flavor and color attributes is achieved, while excessive addition of more than 17.6% results in aldehyde overaccumulation and accelerated browning.

Research Progress On The Extraction Of Functional Components From Chinese Baijiu Distiller's Grains And Their High-Value Applications, Wang Yanyong, Guo Shuanghai

Food and Machinery

Chinese Baijiu distiller's grains represent a major by-product of the Baijiu brewing industry. The solid-state or liquid-state multi-strain fermentation process endows them with complex chemical compositions and functional properties, conferring considerable potential for further development. This study describes the chemical composition of Chinese Baijiu distiller's grains, discusses the differences in functional components arising from the brewing processes of various Baijiu aroma types, and evaluates advanced extraction techniques targeting core components, including proteins, dietary fibers, and polyphenols. The biological activities of Chinese Baijiu distiller's grain extracts, such as antioxidant, antihypertensive, and immunoregulatory effects, as well as their underlying mechanisms of action, …

Invasive African Fig Flies (Zaprionus Indianus) Have Reduced Cold Acclimation Ability Compared To Cosmopolitan Drosophilid Species, Mia Hollekim, Lucy Smith, Priscilla A. Erickson

Biology Faculty Publications

Zaprionus indianus, an invasive species of fruit fly originating from Africa, has successfully inhabited eastern North America but is thought to be seasonally limited by cold temperatures. We compared the cold acclimation abilities of Z. indianus to six drosophilid species. Each species’ survival at -5°C was tested following acclimation periods at 10°C, 15°C, and 25°C. We found that Z. indianus had a similar baseline cold tolerance to other cosmopolitan species and acclimated following treatment at 15°C. Overall, cold acclimation in Z. indianus was better than tropical species but weaker than some cosmopolitan species, suggesting cold may restrict Z. indianus …

Advances In Extraction And Detection Technologies For Veterinary Drug Residues In Food, Luo Qiaoqiao, Yuan Yufeng, Jia Yunzhu, Chen Qingya, Tan Fang, Zhao Xin

Food and Machinery

Veterinary drug residues constitute a global challenge that poses significant threats to food safety and public health. This review systematically summarizes recent advances in detection technologies for veterinary drug residues in food, with a particular focus on the performance characteristics and application status of sample extraction techniques (including liquid-liquid extraction, solid phase extraction, accelerated solvent extraction, QuEChERS, and magnetic solid phase microextraction ),as well as analytical methods (including chromatographic-mass spectrometric techniques, immunoassays, capillary electrophoresis, biosensors, and quantum dots -based approaches ). Comparative analysis reveals that accelerated solvent extraction (ASE ) and QuEChERS have emerged as mainstream pretreatment techniques owing to …

Dual-Action Antibacterial Agents Targeting Biofilm Reduction And Membrane Disruption In Staphylococcus Aureus, Rahmat Folashade Zakariyah, Waliyullahi Akorede Husain, Elohor Precious Samuel, Wonder Olanipekun Oloruntoba, Fatmah Opeyemi Saka, Khalid Ayinde Oyelami, Mariam Temitope Baker

Al-Bahir

Antimicrobial resistance in Staphylococcus aureus is a serious global health problem as the organism rapidly develops resistance against widely used antibiotics. This study sought to investigate the antibacterial and antibiofilm properties of γ-Tocopherol, a natural compound from Ocimum gratissimum, as well as its synergism with 5-hydroxymethylfurfural (5-HMF) against S. aureus. The antibacterial activities and minimum inhibitory concentrations of the compounds were determined using agar diffusion and microbroth dilution methods, respectively. Biofilm reduction was evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) assay and membrane integrity was evaluated using the crystal violet microtiter plate assay and the ion leakage assay. The results …

A Rho5-Cnc1-Dnm1 Signalling Module Coordinates Multi-Organelle Execution Of Regulated Cell Death In Yeast, Justin Bauer

Theses and Dissertations

Regulated cell death is a conserved process with major implications for health and disease, yet how yeast cells integrate stress signals to determine survival versus death remains unclear. This thesis investigates Cyclin C (Cnc1), a conserved Mediator kinase module component with both transcriptional and mitochondrial roles. Together with Cdk8 Cnc1 primarily represses stress response genes, while upon stress it translocates to the mitochondria to induce mitochondrial fragmentation with the dynamin-related GTPase Dnm1. Using structure-guided separation-of-function mutants, I demonstrate that Cnc1-dependent death requires its mitochondrial function rather than its transcriptional role, and that its effector Dnm1 acts as a signaling node …

The Helminthic Status Of Selected Tanzanian Subterranean Rodents, The Root-Rats (Spalacidae: Tachyoryctes Splendens) And Emini Silvery Mole-Rats (Bathyergidae: Heliophobius Argenteocinereus Emini), Jestina V. Katandukila

Tanzania Journal of Science

Root-rats (Tachyoryctes splendens) and emini silvery mole-rats (Heliophobius argenteocinereus emini) are common subterranean rodents that are essential component of the ecosystem. They serve as prey for small to medium-sized carnivores and birds of prey but their role as hosts to parasites is limited. The present study, explored the Gastrointestinal Tract (GIT) contents of 40 rodents (20 T. splendens and 20 H. a. emini, respectively) to acquire information on parasites diversity of these mammals. The GIT contents from subterranean rodents in a sex ratio of 1:1 were examined under a compound microscope to isolate helminths. The isolated helminths belonged to the …

Impact On Energy Budgets In Grassland Production And Conservation Of Possible Production Of Energy From Biomass Crops, R J. Wilkins

IGC Proceedings (1977-2023)

There is commonly a conflict between output/unit of area of land and output/unit of support-energy (SE) input. The possibil­ity of using a proportion of the total land area for biomass energy production suggests an approach for calculating grassland out­put/ha unconfounded with differences in SE input. Output can be calculated for an area of land that is devoted to grassland and biomass energy crops in proportions that result in a net demand for SE of zero. Outputs of utilized metabolizable energy (UME) are related to inputs of SE for (1) grazed grass with high application of nitrogen (N) fertilizer and (2) …

Efficiency Of Dairying Under Contrasting Feeding And Management Systems In North America, Israel, Europe, And New Zealand, J.D. J. Scott

IGC Proceedings (1977-2023)

Four different systems of feeding and managing dairy cattle are seen in major milk-producing countries. These are (1) feeding purchased roughages and concentrates to zero-grazed, intensively penned cows; (2) home growing of roughages and most con­centrates for zero-grazed animals; (3) feeding concentrates with conserved pasture and other roughages in winter and reduced concentrates to grazed cattle in spring-summer; and (4) feeding cattle on grazed pasture and conserved pasture throughout the year. The following data on the four systems were obtained from examples in southern California, Wisconsin, England/Wales, and New Zealand. Average fat-corrected milk (FCM)/cow is approximately 7,140, 5,300, 4,670, and …

Potential Contribution Of White Clover To The Economics And Support-Energy Inputs Of Grass-Based Beef-Production Systems, T A. Stewart, R E. Haycock, A S. Laidlaw

IGC Proceedings (1977-2023)

Biological output, gross margins, and efficiency of support-energy usage in an 18-month beef-production system on grass-white clover swards receiving either 50 kg N/ha stocked at 3.3 head/ha (low N) or 300 kg N/ha stocked 4.5 head/ha (high N) were compared. To date, two production cycles (1977-1979 and 1978-1980) at two sites have been completed. Mean live-weight and carcass gains/ha were, respectively, 17.5% and 14% higher from the high-N than from the low-N system. Gross margin/head was higher from the high-N system in the first production cycle but not in the second. In the low-N system as com· pared with the …

An Evaluation Of The Black Walnut- Tall Fescue Pasture-Management System, H E. Garrett, W B. Kurtz

IGC Proceedings (1977-2023)

The economic and biological feasibility. of a black walnut-livestock-grazing multicropping-management regime bas been evaluated for comparison with a similar livestock-management regime conducted in the open. Pure tall fescue plots were established under open and black walnut-shade conditions. Forage yields on a dry-weight basis were estimated for a 3-year period. A concurrent grazing trial was co􀀌ducted on similar sites using 1.2-ha plots. Black walnut seedlings were planted within the plots on a 3 x 12-m spacing and protected from grazing animals by electric fencing. The economic analyses of the alternate grazing systems were based on their respective present net worth and …

Economic Analysis Of The Impact Of Management Decisions On Production And Use Of Silage For A Dairy Enterprise, A J. Corrall, H D. St. C Neal, I M. Wilkinson

IGC Proceedings (1977-2023)

The aim of this work was to examine the influence of variations in grass management and methods of ensiling on the economics of milk production for autumn-calving cows. A simulation model was developed to combine knowledge of plant and animal responses and their interactions in order to calculate the effects of management options on economic balances. Input data were compiled for herbage production from cut­ting strategies that gave herbage at four digestibilities, at two levels of applied nitrogen, and in three climates. Experimental evidence was also collated to construct matrices of loss of dry matter and changes in digestibility and …

Effective Training For International Students, G W. Thomas

IGC Proceedings (1977-2023)

The observations made in this paper are based upon contacts with international students, both in the U.S. and in many other countries. Two of the most serious problems in increasing world food production in the developing world are (1) lack of trained people, and (2) technology transfer. A third constraint, inadequate research, is also critical but not of the magnitude of the other two, since there is a great amount of research information available that is not being adequately ut:ilized. Training needs fall into two general categories in which deficiencies are obvious: (1) graduate-level programs, particularly in the applied fields …

West Virginia University Allegheny Highlands Project: A Ten-Year Experiment In Technology Transfer, B S. Baker, P E. Lewis, E K. Inskeep, R H. Maxwell

IGC Proceedings (1977-2023)

West Virginia's livestock industry is characterized by relatively small units located predominantly on rough terrain not suited to highly mechanized or intensive agriculture. As a result of changes in West Virginia agriculture during the last 40 years, there has been a reduction in support from agribusiness, marketing alternatives, and profitability. Some agriculturalists contend that if present technology were adopted, beef and sheep farms in mountainous regions could be profitable and could contribute to the local economy. The objective of the Allegheny Highlands Project (AHP) was to test the effectiveness of a method for delivering technological information to farmers to promote …

Grassland Societies In Manitoba, G Bonnefoy

IGC Proceedings (1977-2023)

Farmers with forage and livestock problems were organized into grassland societies and cooperated with extension staff sup­plied by 􀀇anitoba Agriculture. The purpose of the grassland societies is to improve by demonstration the traditional manage­ment practices used by livestock producers for pastures and forage production for beef and dairy herds. Results over 5 years were clearly positive. Significant improvements were noted in both field and animal husbandry practices. The experience confirmed the principles of the diffusion-adoption process and its application to adult extension education.

Pasture Demonstrations For Beef: Agronomic Considerations, N P. Martin, P R. Hasbargen, R L. Arthaud, G J. Sullivan

IGC Proceedings (1977-2023)

Beef cattle supply a major portion of farm income of northern Minnesota, but the major resource supporting this enterprise, forage, is vastly underutilized. A 5-year on-the-farm demonstration was conducted by the Agricultural Extension Service of the University of Minnesota and the Upper Great Lakes Regional Commission to improve income in this area by improving man­agement of the beef enterprise.

We compared one or two improved pasture systems with an unimproved permanent grass pasture composed predominantly of Kentucky bluegrass (Poa pratensis L.), system A, on each of six ranches. Improved systems included use of a cool-season grass fertilized with 138 kg …

Optimizing The Use Of Grazed Dryland Pasture In Western Oregon, T E. Bedell

IGC Proceedings (1977-2023)

When they are properly fertilized, western Oregon dryland pastures of subterranean dover with grasses such as perennial rp:g:rass, tall fescue, and resident annuals produce total annual dry-matter yields averaging 6,500 kg/ha. Some sites are capable of over 9,000 kg/ha. Optimum use of this forage is complicated by its highly seasonal availability. Over 50 % of the total growth occurs from late April to mid-June. Only about 10% takes place during autumn-early winter, and virtually none occurs during summer. Research shows livestock weight gains from 300 to over 700 kg/ha can be expected depending upon pasture mixture and kind of grazing …

Growing Beef Cattle On Native Pasture Oversown With The Legume Fine-Stem Stylo In Subtropical Australia, K G. Rickert, G M. Mckeon, J H. Prinsen

IGC Proceedings (1977-2023)

Fine-stem stylo (FSS), Stylosanthes guianensis var. lntermedia, is a perennial legume suitable for sowing into native Heteropogon contortus pastures on well-drained soils in southeastern Queensland. A grazing system is described that aims to maintain a productive FSS pasture and grow steers to 480 kg live weight at 27 months of age. This goal requires gains of 300 kg/steer in 20 months after weaning. Results from a short-term field study of the system were analyzed for long-term repeatabil­ity using a simple model that predicted animal production from stocking rate and weather records. The system was studied near Gayndah, Queensland, on 11.3 …

Development Of Legume-Based Pastures For Beef Production In Dry Tropical Areas Of Northern Australia, J G. Mcivor, R J. Jones, C J. Gardener, W H. Winter

IGC Proceedings (1977-2023)

A program designed to select pasture legume species suitable for beef cattle grazing in the 500-1,500 mm annual rainfall zone of northern Australia is described. A detailed study of members of the genus Stylosanthes, involving spaced-plant studies and small-scale cutting trials at a number of sites, identified a number of persistent, high-yielding accessions. Animal-production studies have shown that the introduction of a legume into native pasture generally results in an increase in carrying capacity, although production/head may not increase unless superphosphate is applied. The Stylosanthes accessions can produce moderate to high yields without superphosphate, and only small applications of superphosphate …

Development Of A Pasture Research Program For The Tropical Savanna Region Of Brazil, D Thomas, C P. Moore, W Couto, R P. De Andrade, C.M C. Da Rocha, D T. Gomes

IGC Proceedings (1977-2023)

The tropical savannas (cerrados) of west central Brazil contain almost 40% of the nation's cattle population. However, the low productivity and nutritive value of the native pastures is a serious limitation to cattle production. To improve the nutrition of the grazing animal, a research program has been developed with emphasis on the integrated use of cultivated pastures. The program aims (1) to select species adapted to the cerrados, (2) to determine their nutrient requirements, and (3) to develop methods of establishment and utilization. The program is being conducted at the Cerrados Agricultural Research Center (CP AC) at lat 15°S. Rainfall …

Advances In Pasture Research And Development In Santa Cruz, Bolivia, R T. Paterson, C R. Horrell

IGC Proceedings (1977-2023)

Cattle production is important in Santa Cruz but is low in productivity, due in part to low grazing value of natural pastures, particularly in the dry season. Other constraints to cattle production include low land values, poor management, high cost of in­puts, and relatively low product prices. This program is aimed at providing higher-quality, low-cost pastures for complemen­tary use within traditional grazing systems. Improved introduced species were identified for five agro-ecological zones, and commercial seed production of the following was encouraged: Petri guineagrass (Panicum maximum), Brachiaria decumbens, buf­felgrass (Cenchrus ciliaris), glycine (Neonotonia wightii, formerly Grycine wightii), Archer axillaris (Macroryloma axillare), …

Pasture Research Results In The Brazilian Amazon, E.A S. Serrao

IGC Proceedings (1977-2023)

Natural upland savanna grasslands are represented mainly by well-drained savannas, with the cerrado type predominant, and by poorly drained savannas with the campo alto-type being the most common. The main limitations are low forage­production potential and, especially, low forage quality. About 3 million ha of rain forest has been replaced by improved pastures of guineagrass (Panicum maximum, 80% ), jaraguagrass (Hyparrhenia rufa, 10% ), and Brachiaria spp. and other grasses (10% ). During the first few years after establish­ment, pastures are productive. However, with time, a gradual decline of productivity occurs, especially in P. maximum pastures. About 0.5 million ha …