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Бадхызские Архивы. Том 2, Р. И. Горелова, В. Я. Фет

Open Access Books

Gorelova, RI, Fet V, editors. The Badkhyz Archives, Vol. 2. Huntington, WV: Marshall University Libraries; 2025. 387 p.

This book represents the second volume of the series started in 2021. It compiles memoirs and research information about the unique Badkhyz Natural Reserve, now in Turkmenistan, formerly at the southernmost point of the USSR. Established in 1941, the Reserve—nicknamed a Serengeti of Central Asia— housed many unique and endangered species, with an iconic wild ass, or kulan, preserved only there. The second volume includes unique and otherwise unavailable information about the workers of the Reserve in 1940s-1980s. We continuento publish …

Antibiotic-Induced Loss Of Gut Microbiome Metabolic Output Correlates With Clinical Responses To Car T-Cell Therapy, Rishika Prasad, Abdur Rehman, Lubna Rehman, Faezeh Darbaniyan, Viktoria Blumenberg, Maria-Luisa Schubert, Uria Mor, Eli Zamir, Sabine Schmidt, Tomo Hayase, Chia-Chi Chang, Lauren Mcdaniel, Ivonne Flores, Paolo Strati, Ranjit Nair, Dai Chihara, Luis E Fayad, Sairah Ahmed, Swaminathan P Iyer, Michael Wang, Preetesh Jain, Loretta J Nastoupil, Jason Westin, Reetakshi Arora, Joel Turner, Fareed Khawaja, Ranran Wu, Jennifer B Dennison, Meghan Menges, Melanie Hidalgo-Vargas, Kayla Reid, Marco L Davila, Peter Dreger, Felix Korell, Anita Schmitt, Mark R Tanner, Richard E Champlin, Christopher R Flowers, Elizabeth J Shpall, Samir Hanash, Sattva S Neelapu, Michael Schmitt, Marion Subklewe, Johannes Francois-Fahrmann, C K Stein-Thoeringer, Eran Elinav, Michael D Jain, Eiko Hayase, Robert R Jenq, Neeraj Y Saini

Faculty, Staff and Student Publications

Antibiotic (ABX)–induced microbiome dysbiosis is widespread in oncology, adversely affecting outcomes and side effects of various cancer treatments, including immune checkpoint inhibitors and chimeric antigen receptor T-cell (CAR-T) therapies. In this study, we observed that prior exposure to broad-spectrum ABXs with extended anaerobic coverage such as piperacillin-tazobactam and meropenem was associated with worse anti-CD19 CAR-T therapy survival outcomes in patients with large B-cell lymphoma (N = 422) than other ABX classes. In a discovery subset of these patients (n = 67), we found that the use of these ABXs was in turn associated with substantial dysbiosis of gut microbiome function, …

Aif3 Splicing Variant Elicits Mitochondrial Malfunction Via The Concurrent Dysregulation Of Electron Transport Chain And Glutathione-Redox Homeostasis, Mi Zhou, Shuiqiao Liu, Yanan Wang, Bo Zhang, Ming Zhu, Jennifer E Wang, Veena Rajaram, Yisheng Fang, Weibo Luo, Yingfei Wang

Faculty, Staff and Student Publications

Genetic mutations in apoptosis-inducing factor (AIF) have a strong association with mitochondrial disorders; however, little is known about the aberrant splicing variants in affected patients and how these variants contribute to mitochondrial dysfunction and brain development defects. We identified pathologic AIF3/AIF3-like splicing variants in postmortem brain tissues of pediatric individuals with mitochondrial disorders. Mutations in AIFM1 exon-2/3 increase splicing risks. AIF3-splicing disrupts mitochondrial complexes, membrane potential, and respiration, causing brain development defects. Mechanistically, AIF is a mammalian NAD(P)H dehydrogenase and possesses glutathione reductase activity controlling respiratory chain functions and glutathione regeneration. Conversely, AIF3, lacking these activities, disassembles mitochondrial complexes, increases …

Wild Licorice (Glycyrrhiza Lepidota) Identification And Management In Pastures, Jacob Hadfield, Taun Beddes, Michael Caron, Corey Ransom

All Current Publications

Wild licorice, also known as American licorice, is a perennial legume native to North America, commonly found in the western United States. Wild licorice reproduces rapidly through seeds and rhizomes, leading to large infestations, particularly in pastures. The low preference of livestock for wild licorice results in overgrazing of other plant species, leaving them at a competitive disadvantage, further promoting the spread of wild licorice and contributing to pasture infestations. This fact sheet reviews the identification and management of wild licorice in pastures.

Phylogenetic Allometric Scaling Of Near Basal Breathing Frequency In Terrestrial, Semi-Aquatic And Aquatic Mammals, Andreas Fahlman, Elliot Stielstra, Ethan Wilstermann, Simon Rylaarsdam, Josefin Larsson, Guillermo J. Sanchez -Contreras, Suguru Higa, Gonçalo N. Marques, Malgosia Kaczmarska, Jason Somarelli, Stacy L. De Ruiter

University Faculty Publications and Creative Works

We measured the BASAL breathing frequency following an overnight fast in adult,non-pregnant/non-lactating, inactive mammals ranging in body mass from 15 to5520 kg. The data included results from 338 individual animals from 34 speciesthat were divided into terrestrial, semi-aquatic (Otariidae and Phocidae) and aquaticmammals. Following attempts to limit the collection of breathing frequency usinga basal definition and to correct the analysis phylogenetically, our results suggestthat there are differences in the allometric mass-exponent between terrestrial andaquatic/semi-aquatic mammals. An allometric regression model, whereby both bodymass and breathing frequency were transformed using log10 , suggested that theallometric mass exponent for terrestrial mammals (−0.303) was …

Consumer Acceptance Of Gene-Edited Food: The Role Of Knowledge, Trust, And Information, Anubrata Deka, Syed Imran Ali Meerza, Amalia Yiannaka

Cornhusker Economics

The research aims to assess consumer preferences and attitudes toward CRISPR applications in the food sector and estimate consumer willingness to pay for a food product developed using CRISPR technology to enhance food safety and confer health benefits. Additionally, the research assesses the impact of information provision on perceptions, attitudes and willingness to pay. Specifically, we consider the potential effect of both the nature of information provided (i.e., whether it emphasizes the differences between GM and CRISPR technology or not) and the delivery format (i.e., text versus video) used to present content-equivalent information. In this context, we also assess consumer …

Effect Of Multispecies Swards On The Fat Composition And Oxidative Stability Of Milk From Dairy Cows In Mid-Late Lactation, Samuel Rapisarda, Kate M. Mccarthy, Tommy M. Boland, Helen Sheridan, Frank J. Monahan, Finbar J. Mulligan, Graham O'Neill, Nissreen Abu-Ghannam

Articles

Multispecies swards have been recognized as more sustainable than traditional ryegrass systems, in addition to providing a range of phytochemicals to the ruminant diet. In this regard, polyphenols may be linked to higher levels of bioactive fatty acids in milk and enhanced nutraceutical properties in dairy products. This study compares the fat composition and oxidative stability of milk produced by cows fed on multispecies swards (MULTI) with milk from two conventional swards [a perennial ryegrass monoculture (PRG) and a mixture of perennial ryegrass and white clover (PRG + WC)] during the Irish summer. Milk from cows fed on MULTI had …

Identification Of Novel Argonaute Proteins Using A Metagenomic Mining Approach, Lobna Abdallah Ghonaim

Theses and Dissertations

Gene editing is one of the most promising tools in science. It enables precise modifications of an organism's genetic material. Metagenomics is considered a powerful tool that unlocks the broad genetic potential found in uncultured microbial communities. Exploring the genetic diversity of uncultured microbial communities helps identify novel functional proteins with unique properties and make the best use of these diverse microbial ecosystems.

We developed and employed a metagenomic-based approach to mine more than 1000 metagenomes for prokaryotic argonaute proteins (pAgos), a potential gene editing machinery encoded in bacterial and archaeal genomes. Our workflow involved strict quality control, sequence assembly, …

Development Of A Conditional Plasmid For Gene Deletion In Non-Model Fusobacterium Nucleatum Strains, Peng Zhou, Bibek G C, Chenggang Wu

Faculty, Staff and Student Publications

Fusobacterium nucleatum is an opportunistic pathogen with four subspecies: nucleatum (FNN), vincentii (FNV), polymorphum (FNP), and animalis (FNA), each with distinct disease potentials. Research on fusobacterial pathogenesis has mainly focused on the model strain ATCC 23726 from FNN. However, this narrow focus may overlook significant behaviors of other FNN strains and those from other subspecies, given the genetic and phenotypic diversity within F. nucleatum. While ATCC 23726 is highly transformable, most other Fusobacterium strains exhibit low transformation efficiency, complicating traditional gene deletion methods that rely on non-replicating plasmids. To address this, we developed a conditional plasmid system in which …

The Role Of Non-Coding Rnas As An Innovative Therapeutic Tool For Hepatocellular Carcinoma, Nabila Mohamed Hosni El Kramani

Theses and Dissertations

Hepatocellular carcinoma (HCC) is the second lethal cancer worldwide, with over 60 million new patients added annually. Hepatitis B infection and toxins, e.g., aflatoxin, contribute to the incidence of HCC. Egypt and China show the highest prevalence of viral hepatitis and HCC in the world.

Long non-coding RNAs (lncRNAs) are non-protein-coding transcripts larger than 200 nucleotides. These transcripts work at the transcriptional, post-transcriptional, and epigenetic levels to regulate gene expression by interacting with various biomolecules, such as RNA, DNA, and proteins. They serve essential functions in chromatin remodeling and cell survival, differentiation, and proliferation. The role and biological interactions of …

Rural Entrepreneurship In Upper Egyptian Communities: Exploring The Intervention Of Agri-Tech Startups, Marwa Wael Abohamda Hulail

Theses and Dissertations

Climate change and economic disturbance have changed the state of the world and increased the dependence on digitization in running people's daily life. In Egypt, the economic instability and climate change have affected people’s standard of living, making it challenging to sustain their life, especially in the agriculture sector. Farmers have been affected by the risks of climate change, economic shocks, and trends of digitization, which have impacted their profit, employability, and livelihood.

Several Egyptian Agri-tech startups were launched to support the farmers in the digital revolution that came to agriculture, attempting to make a change in the Egyptian farming …

Evaluation Of A Global Capacity Development Project To Educate, Empower And Advance Sustainable Food Production, Felicia J. Tuggle, Olubusola W. Afolayan Msw, Mollie Smith M.Aq., David J. Cline Phd, Onesimus Kiadii Bs

Journal of Social Work in the Global Community

This article presents evaluation findings from the Integrated Aquaculture for Food Production, Education, Empowerment, and Development (iFEED) project, a global capacity development project designed to educate, empower, and advance sustainable food production in Monrovia, Liberia. To develop K–12 teachers’ capacity to integrate STEM education into math and science curriculum for the purpose of preparing a future aquaculture workforce in Liberia, the iFEED project provided educational materials, teacher training, and technical support to 25 teachers in the greater Monrovia Consolidated School District (MCSS). Evaluation findings indicate positive outcomes in increasing instructional capabilities among participating math and science teachers and accelerating action …

“Phytochemical Analysis, In Vitro Anti-Oxidant, Anti-Diabetic And Anti-Inflammatory Activity Of Red Kidney Bean (Phaseolus Vulgaris L.) ”, Nancy Mani, D Annette Beatrice, R Durga Priyadarshini

International Journal of Health and Allied Sciences

Aims: The aim of the study is to assess the phytochemicals, bioactive compounds and determine the in vitro anti-oxidant, anti-diabetic and anti-inflammatory potential of red kidney bean.

Methods and Material: Raw and cooked red kidney bean (Phaseolus vulgaris. L) were assessed for phytochemical content, anti-oxidant, anti-diabetic and anti-inflammatory activity and GC-MS analysis was done to identify the bioactive compounds.

Statistical analysis used: Descriptive statistics, independent ‘t’ test and one-way ANOVA was computed to determine the differences in the phytochemical compounds and in vitro activities of raw and cooked red kidney beans.

Results: Qualitative analysis of raw and …

Single-Cell Analysis Of Neoplastic Plasma Cells Identifies Myeloma Pathobiology Mediators And Potential Targets, Luz Yurany Moreno Rueda, Hua Wang, Keiko Akagi, Minghao Dang, Amishi Vora, Li Qin, Hans C Lee, Krina K Patel, Pei Lin, David E Mery, Fenghuang Zhan, John D Shaughnessy, Qing Yi, Yang Song, Bo Jiang, Maura L Gillison, Sheeba K Thomas, Donna M Weber, Lixia Diao, Jing Wang, Isere Kuiatse, Elisabet E Manasanch, David E Symer, Robert Z Orlowski

Faculty, Staff and Student Publications

Multiple myeloma is a clonal plasma cell (PC) dyscrasia that arises from precursors and has been studied utilizing approaches focused on CD138+ cells. By combining single-cell RNA sequencing (scRNA-seq) with scB-cell receptor sequencing (scBCR-seq), we differentiate monoclonal/neoplastic from polyclonal/normal PCs and find more dysregulated genes, especially in precursor patients, than we would have by analyzing bulk PCs. To determine whether this approach can identify oncogenes that contribute to disease pathobiology, mitotic arrest deficient-2 like-1 (MAD2L1) and S-adenosylmethionine synthase isoform type-2 (MAT2A) are validated as targets with drug-like molecules that suppress myeloma growth in preclinical models. Moreover, functional studies show a …

Mitochondrial Defects And Metabolic Vulnerabilities In Lynch Syndrome-Associated Msh2-Deficient Endometrial Cancer, Mikayla Borthwick Bowen, Brenda Melendez, Qian Zhang, Diana Moreno, Leah Peralta, Wai Kin Chan, Collene Jeter, Lin Tan, M Anna Zal, Philip L Lorenzi, Kenneth Dunner, Richard K Yang, Russell R Broaddus, Joseph Celestino, Nisha Gokul, Elizabeth Whitley, Deena M Scoville, Tae Hoon Kim, Jae-Wook Jeong, Rosemarie Schmandt, Karen Lu, Hyun-Eui Kim, Melinda S Yates

Faculty, Staff and Student Publications

Lynch syndrome (LS), caused by inherited mutations in DNA mismatch repair genes, including MSH2, carries a 60% lifetime risk of developing endometrial cancer (EC). Beyond hypermutability, mechanisms driving LS-associated EC (LS-EC) remain unclear. We investigated MSH2 loss in EC pathogenesis using a mouse model (PR-Cre Msh2LoxP/LoxP, abbreviated Msh2KO), primary cell lines, human tissues, and human EC cells with isogenic MSH2 knockdown. By 8 months, 58% of Msh2KO mice developed endometrial atypical hyperplasia (AH), a precancerous lesion. At 12-16 months, 50% of Msh2KO mice exhibited either AH or ECs with histologic similarities to human LS-ECs. Transcriptomic profiling of EC from Msh2KO …

Natural And Bioengineered Extracellular Vesicles In Diagnosis, Monitoring And Treatment Of Cancer, Xin Luo, Kathleen M Mcandrews, Raghu Kalluri

Faculty, Staff and Student Publications

Extracellular vesicles (EVs) are cell derived nanovesicles which are implicated in both physiological and pathological intercellular communication, including the initiation, progression, and metastasis of cancer. The exchange of biomolecules between stromal cells and cancer cells via EVs can provide a window to monitor cancer development in real time for better diagnostic and interventional strategies. In addition, the process of secretion and internalization of EVs by stromal and cancer cells in the tumor microenvironment (TME) can be exploited for delivering therapeutics. EVs have the potential to provide a targeted, biocompatible, and efficient delivery platform for the treatment of cancer and other …

Nanrilkefusp Alfa (Sot101), An Il-15 Receptor Βγ Superagonist, As A Single Agent Or With Anti-Pd-1 In Patients With Advanced Cancers, Stephane Champiat, Elena Garralda, Vladimir Galvao, Philippe A Cassier, Carlos Gomez-Roca, Iphigenie Korakis, Peter Grell, Aung Naing, Patricia Lorusso, Romana Mikyskova, Nada Podzimkova, Milan Reinis, Kaissa Ouali, Andreu Schoenenberger, Joachim Kiemle-Kallee, Sascha Tillmanns, Richard Sachse, Ulrich Moebius, Radek Spisek, David Bechard, Lenka Palova Jelinkova, Irena Adkins, Aurelien Marabelle

Faculty, Staff and Student Publications

Nanrilkefusp alfa (nanril; SOT101) is an interleukin (IL)-15 receptor βγ superagonist that stimulates natural killer (NK) and CD8

Scorpions Of The Horn Of Africa (Arachnida: Scorpiones). Part Xxxv. Parabuthus Starhai Sp. N. From Somalia (Puntland) (Buthidae), František Kovařík

Euscorpius

A new species Parabuthus starhai sp. n. from Somalia (Puntland) is described. Combination of two characters, pectine teeth number 56–59 in both sexes and pedipalp chela length/ width ratio 3.65 in male and 5.47 in female differ P. starhai sp. n. from all other species in the region. Included is also a map of distribution of Parabuthus species in the Horn of Africa.

Mitochondrial Dysfunction Is A Hallmark Of Woody Breast Myopathy In Broiler Chickens, Elizabeth S. Greene, Paula R. Chen, Carrie Walk, Mike Bedford, Sami Dridi

Poultry Science Faculty Publications and Presentations

The woody breast (WB) myopathy poses significant economic and welfare concerns to the poultry industry, however, there is no effective strategy to mitigate this pathology due to its unknown etiology. After showing previously that hypoxia is a key factor in WB progression, we used here various techniques demonstrating dysregulated mitochondria (morphology, biogenesis, tethering, function, and bioenergetics) in WB-affected muscles and in hypoxic myoblasts compared to healthy tissues and normoxic cells, respectively. The increased levels of calcium (Ca2+) in both WB-affected tissues and hypoxic myoblasts suggested that mitochondrial Ca2+ overload is likely a leading cause for mitochondrial dysfunction that merits further …

Western Australia’S Marine Heatwave Update - 16 February 2025, Department Of Primary Industries And Regional Development, Western Australia

Marine Heatwave Notification Reports

No abstract provided.

Found: Three Trashed Poems, Tanis Macdonald

The Goose

Three poems about garbage, what is leftover and found.

My Art Is Garbage, Beth Shepherd

The Goose

In “My Art is Garbage,” I describe three recent art projects where garbage is both a theme and an artistic medium used to critique the overuse of and inability to reuse or safely dispose of plastics we use every day. The first work, Untitled (Plastic Film Balls), uses non-recyclable single-use household plastic made to form an installation of shimmering balls and alludes to the environmental footprint of the artworld. Plastic Shores, a series of monotype prints, reminds us that plastics in the environment are deleterious to species that we see every day. Finally Plastic Pests, a series …

Beer And Biology, A Perfect Pairing: Using Beer To Spread Awareness About Invasive Species, Samuel Nutile, Adam Simpson, Sara Stahlman, Lynne E. Beaty

Journal of Extension

Managing the spread of introduced invasive species relies on educating recreationists that currently or potentially harbor such species. By partnering with a local brewery, we developed the Lagers for Lakes Draft Series to facilitate outreach about aquatic invasive species in Erie, Pennsylvania. To date, Lagers for Lakes drafts and associated launch events have reached over 230 individuals and increased awareness of invasive species by 86%. With the materials provided here and the lessons learned from these events, we hope similar outreach efforts can be developed elsewhere to educate and minimize the spread of invasive species.

Offal Wildlife Watching: Connecting Hunters, Wildlife Researchers, And The Public Through Participatory Science, Ellen M. Candler, Grace Milanowski, Amy Rager, Joseph K. Bump

Journal of Extension

Offal Wildlife Watching is a participatory science project that engages deer hunters in wildlife research. The goal of the project is to better understand the ecology of scavenger species that visit deer gut piles across Minnesota. We asked hunters to deploy remote cameras at white-tailed deer gut piles that resulted from their freshly killed deer. Thus far 117 hunters have contributed images and recorded at least 49 different scavenger species. This project is a natural fit in Extension programs and can easily be adapted to other hunted species, biomes, and locations.

The New American Farmer – Extension Engagement With Urban Agriculture And Food Systems, Margaux S. C. Robinson, Kc V. Cunningham, Jeffery A. Young, Nicole D. Breazeale, Kenneth R. Jones, Julie N. Zimmerman

Journal of Extension

Extension’s evolving role in urban food production will require intensive reflexivity and ongoing collaboration. Extension educators around the country have already made progress in engaging with both the social and horticultural sides of urban agriculture. Designed appropriately, urban food systems hold the potential for healthy food access, community and environmental resilience, and economic prosperity (Rangarajan & Riordan 2019). Moving forward, we offer recommendations for Extension staff to apply within their institutions and beyond. Specifically, we urge Extension to prioritize the following: 1) mediate the rural/urban dichotomy, 2) tackle structural and institutional power dynamics, and 3) intensify strategies for community resilience.

Single-Cell Rna Sequencing Identifies Molecular Biomarkers Predicting Late Progression To Cdk4/6 Inhibition In Patients With Hr+/Her2- Metastatic Breast Cancer, Linjie Luo, Peng Yang, Sofia Mastoraki, Xiayu Rao, Yan Wang, Nicole M Kettner, Akshara Singareeka Raghavendra, Debasish Tripathy, Senthil Damodaran, Kelly K Hunt, Jing Wang, Ziyi Li, Khandan Keyomarsi

Faculty, Staff and Student Publications

Background: Cyclin-dependent kinase 4/6 inhibitors (CDK4/6is) in combination with endocrine therapy are the standard treatment for patients with hormone receptor-positive, HER2-negative metastatic breast cancer (mBC). Despite the efficacy of CDK4/6is, intrinsic resistance occurs in approximately one-third of patients, highlighting the need for reliable predictive biomarkers.

Methods: Single-cell RNA sequencing analyzed metastatic tumors from HR+/HER2- mBC patients pre-CDK4/6i treatment at baseline (BL) and/or at disease progression. BL samples were from CDK4/6i responders (median progression-free survival [mPFS] = 25.5 months), while progressors were categorized as early-progressors (EP, mPFS = 3 months) and late-progressors (LP, mPFS = 11 months). Metastatic sites included liver, …

Field Observations Of Gopher Tortoise (Gopherus Polyphemus) Activity At A Rare Double-Burrow, James Carroll, Rachel Carroll

Georgia Journal of Science

After a review of the journal, it does not appear that abstracts are included with Research Notes. Therefore, no abstract is provided. However, we certainly defer to the Editors on this requirement and will happily provide an abstract if needed.

Pharmacokinetics Of Liposomal Levamisole After Oral, Intramuscular, And Subcutaneousadministration In Sheep, Hasan Susar, İzzet Karahan

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the pharmacokinetics of free and liposomal levamisole in sheep following single oral (O),intramuscular (IM), and subcutaneous (SC) administrations at a dose of 7.5 mg/kg. The study developed a levamisole liposome andinvestigated relevant parameters: particle size, zeta potential, polydispersity index, encapsulation efficiency, pH, and morphology. TheBangham method, a thin-film hydration technique, was employed to prepare the liposomal levamisole. Blood samples were collectedby jugular venipuncture in heparinized tubes immediately before each free and liposomal levamisole treatment and at 2.5, 5, 10, 15,30, 60, 90, 120, 240, 360, and 480 min and 12, 16, and 24 h after …

Replacement Of Monensin With A Proprietary Tannin-Blend Additive In Calf-Fed Holstein Steer Diets, Sydney M. Bowman-Schnug, Luke K. Fuerniss, Joe D. Cameron, Jonathon L. Beckett, Muhammad Ahsin, Stephan Van Vliet, Guy D. Hufstedler, Bradley J. Johnson

Nutrition, Dietetics, and Food Sciences Faculty Publications

A proprietary tannin blend was evaluated as a potential ionophore replacement considering traits of feedlot cattle growth performance, carcass traits, and health. Holstein steers (n = 1450; initial shrunk body weight (SBW) = 141.6 ± 34.2 kg.) were blocked by origin and randomly assigned to receive a finishing diet containing one of two treatments: 1) 14,500 mg per head daily of Silvafeed ByProX (SBPX; ByPro/BX blend, Silvateam S.p.A, San Michele Mondovì, Italy) or 2) 180 mg per head daily of monensin (MON; Rumensin™ 90, Elanco, Greenfield, IN). Steers were on trial for 341 ± 11 days. Pen was the …

Inhibition Of Histone Methyltransferase Ezh2 For Immune Interception Of Colorectal Cancer In Lynch Syndrome, Charles M Bowen, Fahriye Duzagac, Abel Martel-Martel, Laura Reyes-Uribe, Mahira Zaheer, Jacklyn Thompson, Nan Deng, Ria Sinha, Soham Mazumdar, Melissa W Taggart, Abhinav K Jain, Elena Tosti, Winfried Edelmann, Krishna M Sinha, Eduardo Vilar

Faculty, Staff and Student Publications

Colorectal precancers in Lynch syndrome (LS) exhibit a distinct immune profile, presenting unique opportunities for developing immune-interception strategies to prevent carcinogenesis. Epigenetic modulation by EZH2 of immune-related genes is implicated in the carcinogenesis of different cancer types, including colorectal cancer. This study utilizes a mouse model of LS and ex vivo colonic organoids to assess the effects of the EZH2 inhibitor GSK503 on immune regulatory pathways, tumorigenesis, and epigenetic reprogramming. Our findings revealed that GSK503 significantly increased CD4+ and CD8+ T cells in both splenocytes and colonic mucosa of treated mice compared with controls. Additionally, a preventive dose of GSK503 …