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Valorisation Potential Of Microalgae As A Sustainable Source Of Lipids And Health Promoting Compounds, Bozena Mccarthy

Theses

The transition toward a circular bioeconomy requires sustainable strategies for managing and valorising food industry effluents while reducing reliance on environmentally damaging raw materials such as palm oil, which is associated with deforestation, biodiversity loss and greenhouse gas (GHGs) emissions. Microalgae represent a promising platform for converting nutrient rich industrial by-products into biomass and high-value compounds for food, nutraceutical and bio-based applications. Dairy processing generates large quantities of whey, creating environmental challenges due to its high organic load. Improved whey treatment and valorisation strategies are therefore essential for enhancing sustainability in food production systems. In this study, the biomass productivity …

An Emergent Seafood Nationalism Takes Hold In The United States, Owen Temby

School of Earth, Environmental, & Marine Sciences Faculty Publications

This paper introduces the concept of ‘seafood nationalism’ to describe how, since 2024–2025, the United States increasingly uses tariffs, trade remedies, food safety enforcement and origin-based rules to favour American seafood over foreign products. Federal actions include Executive Order 14276, Restoring American Seafood Competitiveness, which directs agencies to address unfair trade practices, unsafe imports and to develop a coordinated seafood trade strategy. Additional measures include a tariff package imposing a 10% duty on nearly all seafood imports and 30% on seafood from China, as well as new anti-dumping and countervailing duty orders on warm-water shrimp. This paper presents Gulf …

Effects Of Soil Contamination On The Efficacy Of Antimicrobial Washes For Contaminated In-Shell Pecans, Erin Ramsay

Graduate Theses and Dissertations

A study was done to evaluate the survival of Shiga toxin-producing Escherichia coli (STEC) on directly and soil-inoculated in-shell pecans over a 28-day period, determine STEC reductions after treatment with individual and sequential antimicrobial washes on direct and soil-inoculated in-shell pecans, and determine the cross-contamination potential of the spent pecan washes after a treatment. Pecans were directly and soil-inoculated with an STEC cocktail (O157:H7, O157:NM, O121, and O26). Direct inoculation was achieved by spraying the STEC cocktail on the pecans and homogenizing by hand for 2 min. For the soil-inoculation method, autoclaved pecan tree orchard soil was sprayed with the …

Evaluation Of Loading, Release, And Bioavailability Of Resveratrol Encapsulated In Porous Starch, Yu-Yun Wang

Graduate Theses and Dissertations

Resveratrol (Res) is a polyphenolic compound found in grapes, red wine, and berries, with health benefits such as antioxidant, anti-inflammatory, and cardioprotective properties. The poor stability and bioaccessibility of Res after consumption by humans require the development of delivery systems. Among many delivery systems, porous starch (PS) is a safe, cost-effective, and high in adsorption capacity encapsulation material for drugs and bioactive compounds sensitive to environmental factors like light and heat. Porous rice, corn, and potato starch have different granule sizes, pore volumes, and surface areas. However, the impact of these encapsulation capabilities remains unclear among the three porous starches. …

The Unexpected Role Of Conditionality In Shaping Relational Values: Insights From An Incentive-Based Conservation Program In Bolivia, Brooke Mcwherter, Will Munger, Zhao Ma, Claudia Radel, Laura Zanotti, Jonathan Bauchet, Ricardo Godoy

Department of Agricultural Economics Faculty Publications

As global change accelerates, Payments for Ecosystem Services (PES) programs have become increasingly important tools for addressing the interconnected challenges of land degradation, biodiversity loss, water scarcity, and climate change. PES programs provide incentives to individuals or communities, conditional on people adopting sustainable natural resource management practices to support or increase ecosystem service provisioning. Although conditionality is considered a “PES-defining feature,” little is known about how conditionality is perceived by community members and how such perceptions influence their participation in and experience with PES programs. To address this gap, we conducted an extensive qualitative study of Watershared, an incentive-based conservation …

Evaluation Of Thresholds For Adult Tarnished Plant Bug, Lygus Lineolaris (Beauvois), In Thryvon® Cotton, William Anderson Fletcher

Graduate Theses and Dissertations

Tarnished plant bug (TPB) is the most economically damaging insect pest of Mid-South cotton. TPB reduces yield is reduced by damaging squares, terminal growth, and small bolls, resulting in reduced yield. Currently, cotton grown in the Mid-South averages 1-6 insecticide applications per year for control of TPB. The introduction of ThryvOn® cotton technology provides a new management strategy for TPB control. ThryvOn® technology produces the Bacillus thuringiensis (Bt) protein Mpp51Aa2, which has insecticidal activity on TPB. A field study was conducted at five locations in 2024 and seven locations in 2025 across the Mid-South to evaluate different threshold levels for …

Seasonality Of Bovine Respiratory Disease Incidence, Nasal Temperatures, And The Nasal Microbiome In Receiving Beef Cattle: Understanding The Risk Of The Bovine Respiratory Disease Complex, Robin Ashley Cheek

Graduate Theses and Dissertations

Bovine respiratory disease (BRD) has persistently been the leading cause of morbidity of beef cattle in the United States. Season has been directly associated with respiratory disease incidence and is likely a proxy for weather conditions, which vary by geographical location. The nose is one of the first points of contact between the outside environment and inside the body. The nasal microbiome and its relationship with BRD have been heavily studied within beef cattle, but there are not yet any studies to determine if the environment within the nasal cavity has a role to play in this mechanism. This dissertation …

Proteogenomics Of Hypertrophic Cardiomyopathy Reveals Subtype-Specific Therapy, Ke Ma, Jie Yang, Hongchang Guo, Ping Li, Xiaowei Li, Zhujun Dong, Jing Zhang, Congcong Zhang, Pengli Yang, Chongpei Hua, Shuolin Zhu, Guoqing Li, Jianchao Zhang, Ningyu Ding, Jizheng Wang, Xin-Liang Ma, Zhuofeng Lin, Jianzeng Dong, Yang Li, Yulin Li

Department of Emergency Medicine Faculty Papers

BACKGROUND: Hypertrophic cardiomyopathy (HCM) is a heterogeneous disease with diverse prognosis. The underlying mechanisms remain unknown, resulting in limited risk stratification and therapeutic strategies. This study aimed to elucidate molecular subtypes of HCM through integrated proteogenomic analysis and explore subtype-specific therapeutic strategies.

METHODS: We conducted an integrated proteogenomic analysis of 132 patients with HCM using myocardial samples, incorporating whole-exome sequencing, RNA sequencing, and proteomics. Unsupervised clustering was used to identify HCM subtypes, which were validated in heart tissues and human induced pluripotent stem cell-derived cardiomyocytes from 2 independent HCM subsets. Subtype-specific signatures and pathways were explored, and their causal link …

Antioxidant And Anti-Hemolytic Capacities Of Eucalyptus Camaldulensis Leaves, Achwaa Chbib, Malak Mezher, Mohamad Nasser, Akram Hijazi, Mahmoud I. Khalil

BAU Journal - Science and Technology

The effects of medicinal plants on the prevention of many diseases are due to their chemical composition. Eucalyptus camaldulensis (E. camaldulensis) is a native tree in Australia. Its extracts and essential oils (EOs) are applicable in the domains of pharmaceuticals and medicine. This work determined the anti-hemolytic and antioxidant capacities of the ethanolic (EE), methanolic (ME), and aqueous (AE) extracts from the leaves of Lebanese E. camaldulensis.  The leaves of E. camaldulensis were extracted with ethanol, methanol, and water solvent. Qualitative phytochemical analyses were applied to reveal the compounds of these extracts. The Folin-Ciocalteau and Aluminum chloride …

Afforestation Strategies In Semi-Arid Mediterranean Ecosystems: Experimental Evidence From Chmistar, Lebanon, Ahmad Saker, Mohammad Saleh, Wael Mostafa, Safaa Baydoun, Jamilah Borjac, Dalia El-Badan

BAU Journal - Science and Technology

The success rate of broadleaf tree growth in semi-arid Mediterranean environments is reduced in afforestation operations constrained by water scarcity, shallow soil depth, and intense competition from herbaceous vegetation. This study, conducted between 2022 and 2024 in Chmistar, East Lebanon, using a completely randomized block design, aimed to evaluate the impact of three planting broadleaf trees techniques (traditional digging pits , hoedad tools, and auger digging) and four effective vegetation control (herbicide application, mowing,  mulching, and no control) on the early growth of three native broadleaf tree species (Pistacia palaestina, Quercus calliprinos, and Quercus infectoria ).

The …

The Development Of An In Silico Vaccine Design-Based E-Module To Improve Students' Biological Literacy, Qairinasherin Qwine Ferendi, Rusdi Rusdi, Hanum Isfaeni

Jurnal Pendidikan Sains

Low levels of biological literacy among students, particularly in biotechnology, highlight the need for innovative digital learning media that facilitate meaningful and contextual learning experiences. This study aimed to develop and evaluate an interactive biotechnology e-module based on in silico vaccine design to improve students’ biological literacy. The study employed Educational Design Research (EDR) using the Hannafin and Peck model, which consists of three phases: needs assessment, development, and implementation. The participants included 73 tenth-grade science students and one biology teacher from SMAN 68 Jakarta, Indonesia. The e-module was developed using Canva and Heyzine to create an interactive flipbook integrating …

Lesson-1 On Mni Wiconi (Water Is Life): Understanding The Problem Of Water Contamination, Reycel Jugo, Madhav P. Nepal

iLEARN Teaching Resources

Students investigate a scenario of water contamination in a fictional community. They define the engineering problem of dirty water and explore the cultural and ecological significance of water through the lens of indigenous principles. This lesson introduces students to the concept of water contamination and the need for purification, grounding the learning in a real-world problem and connecting to Indigenous knowledge about the land and water.

Problem-Based Learning (PBL) Scenario: Students receive a letter from a fictional community whose water source has become cloudy and is making people sick. They are tasked with becoming “Water Engineers” to investigate the …

Lesson-2 On Mni Wiconi (Water Is Life): Designing And Testing Solutions (Water Filtration), Reycel Jugo, Madhav P. Nepal

iLEARN Teaching Resources

Students apply the engineering design process to build, test, and refine a simple water filter prototype. This hands-on experiential learning activity focuses on the properties of filtering materials and iterative problem-solving.  In this lesson, students will apply the engineering design process to build and test their own simple water filters. They will use their newfound knowledge to begin developing a practical solution to the problem presented in Lesson 1 - Mni Wiconi: A Water Engineering Challenge for 4th Graders.

Problem-Based Learning (PBL) Scenario: Having identified the problem, students are now tasked with designing and building a prototype water filter …

Developing Biodegradable Packaging Films And Beads From Agricultural Residues, Sandeep Paudel, Santosh Rijal, Srinivas Janaswamy, Madhav P. Nepal

iLEARN Teaching Resources

Agricultural waste is produced in large amounts worldwide. These are often discarded despite being valuable sources of cellulose and renewable, biodegradable products. At the same time, rising environmental concerns about petroleum-based plastic packaging have created demand for sustainable alternatives. Introducing K-12 students to biodegradable packaging materials developed from agricultural waste can enhance understanding of sustainability, basic materials science, and circular bioeconomy concepts while encouraging interest in STEM fields. Another concern is the presence of microplastics and nanoplastics in drinking water, which can pose serious risks to human health. Traditional coagulation agents, such as ferric and aluminum salts, are less effective …

Lesson-3 On Mni Wiconi (Water Is Life): Analyzing Evidence And Communicating Solutions (Community Stewardship), Reycel Jugo, Madhav P. Nepal

iLEARN Teaching Resources

Students analyze the data collected from their filter tests, construct an evidence-based argument for their most effective design, and communicate their solution to a “community council” (the class), connecting their technological solution back to community well-being. In the final lesson, students will analyze their data, draw conclusions, and communicate their findings. They will present their improved water filter designs to the class, acting as consultants for the fictional community.

Problem-Based Learning (PBL) Scenario: Students are now ready to present their final water filter prototypes and a summary of their findings to a “community council” (the rest of the class). They …

Protective Effects Of Yogurt Against Sodium Fluoride-Induced Liver Dysfunction In Rabbits, Rasool Bux, Rehana Buriro, Shamsuddin Bughio, Kashif Ali Malak, Muhammad Aqeel, Fayaz Ali, Ali Raza, Abdul Sallam

Journal of Bioresource Management

Fluoride (F-) is among the most important compounds that can damage hepatic tissue and increase level of ALT, ALP, Bilirubin, GGT, and decreases the albumin. Present study was conducted to explore protective role of yogurt on hepatic dysfunction induced by sodium F- at different doses. Animal were divided into seven experimental groups (6 rabbits/group). Group A (Control), Group B (F- 50 mg/kg), Group C (yogurt 15 g/kg), Group D (F- 50 mg + yogurt 15 g/kg), Group E (F- 100 mg/kg), Group F (yogurt 30 g/kg), Group G (F- 100 mg/kg B.W. + yogurt 30 g/kg). In F- treatment groups …

The Multifaceted Medicinal Properties Of Momordica Charantia: Insights Into Phytochemistry And Therapeutic Applications, Maham Ashfaq, Haroon Ur Rashid, Javaria Ashfaq, Shazia Ramzan, Fatma Hussain

Journal of Bioresource Management

Momordica charantia (bitter melon) is a significant medicinal plant, having been long used in traditional medicine in Asian, African and South American systems. M. charantia is more than a traditional medicine; by consolidating existing evidence, it is a promising source of drug discovery and can serve as a complementary in contemporary therapeutics. Over the last several decades, it has become the subject of contemporary biomedical studies due to its abundance of phytochemicals, such as saponins, flavonoids, alkaloids, and polypeptides. These bioactive compounds present a wide range of pharmacological processes that M. charantia can be a natural solution to some of …

Dynamical Behavior, Extinction And Persistence In A Stochastic Delayed Two-Strain Epidemic Model With A Generalized Crowley-Martin Incidence Rate, Amina Allali, Dounia Bentaleb, Saida Amine

Mathematical Modelling and Numerical Simulation with Applications

This paper develops and analyzes a novel delayed stochastic SIR epidemic model with two interacting strains and general incidence functions. By establishing the existence and uniqueness of a positive global solution, the well-posedness of the model under stochastic perturbations is ensured. Extinction occurs when the stochastic reproduction number falls below unity, as demonstrated through Itô calculus and martingale convergence theorems, while persistence is guaranteed under the Crowley–Martin incidence when it exceeds one. Numerical experiments based on the Positive Preserving Truncated Euler–Maruyama (PPTEM) scheme confirm the analytical predictions and highlight the influence of time delay and noise intensity on the long-term …

Crispr/Cas9-Based Genome Editing: Understanding Differences In Dna Repair Pathways, Profiles, And Outcomes, Samuel Effah, Shirley Barrera, Nahia Urturi Ortiz, Will Dampier, Michael Nonnemacher, Brian Wigdahl

Kimmel Cancer Center Faculty Papers

Over a decade of advances in Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) and CRISPR-associated protein 9 (Cas9)-based technologies have culminated in the first-ever FDA-approved CRISPR/Cas-based therapy. Aside from this approved therapy for sickle cell anemia, several CRISPR/Cas-based therapies are currently under development or testing for a range of chronic diseases, including viral diseases like human immunodeficiency virus type 1 (HIV-1) infection, genetic diseases like familial hypercholesterolemia, and cancer. The success of these therapies hinges on the effective delivery of CRISPR/Cas9 components to target regions, efficient Cas endonuclease editing, repair profiles generated, and their resulting outcomes. Here, we discuss the …

Essential And Non-Essential Amino Acid Composition Of Hilsa (Tenualosa Ilisha) Fish In Different Fishing Regions Of Bangladesh, Mohajira Begum, Md. Moshfekus Saleh-E-In, Md. Zia Uddin Al Mamun, Umma Fatema Shahjadee, Farha Matin Juliana, Md. Sabir Hossain

Journal of King Abdulaziz University: Marine Science

Hilsa, Tenualosa ilisha (Hamilton, 1822), is one of the favourite and popular tropical fish in the people of South-East Asia due to its delicious taste and flavour. This study investigates the comprehensive amino acid profiles of various fishing locations of Bangladesh to assess the protein quality and nutritional values. Fourteen free amino acids were identified from the six riverine locations of Bangladesh. The total free amino acid (TAA) contents were ranged from 11.60 g/100 g in Cox's Bazar to 13,40 g/100 g in Kirtonkhola for raw fish. In the case of smoked treated, the TAA ranged from 14.01 g/100 g …

Revolutionizing Coral Research: The Power Of Holo-Omics, Mohammad M. Binsarhan

Journal of King Abdulaziz University: Marine Science

This mini-review highlights the emerging field of holo-omics and its application in unraveling the complex dynamics of the coral holobiont. Corals exist as meta-organisms, closely interacting with a diverse microbiota including bacteria, archaea, microeukaryotes, viruses, and algal symbionts. Understanding these intricate relationships is critical for assessing coral health, stress responses, and resilience to environmental change. We explore the application of meta-omics approaches such as metabarcoding, metagenomics, meta-transcriptomics, metabolomics, and proteomics in profiling coral-associated microbial communities and their functions. The review sheds light on the methodological advantages and limitations of each approach, as well as some of the technical challenges involved …

Microalgae As Sustainable Biofactories For Bioplastic Production: Challenges And Future Perspectives: A Review, Zahraa Hussein Obaid, Noor S. Naji, Jasim M. Salman, Layla Mustafa A.A. Alkatrani

Journal of King Abdulaziz University: Marine Science

The widespread use of traditional plastics, combined with their slow breakdown, has exacerbated environmental and health problems. Therefore, it has become imperative to find alternatives. Due to their simplicity of cultivation, quick growth, tolerance to challenging growing conditions, and low cost, microalgae are being used more frequently as a bio-source for bioplastics lately. Additionally, a variety of biopolymers, including polyhydroxybutyrate, polylactic acid (PLA), starch, polyhydroxyalkanoates (PHA), protein, and cellulose, could be produced by microalgae. These polymers show promising qualities including biodegradability, biocompatibility, and non-toxicity under various cultivation conditions. The purpose of this study is to demonstrate the potential of microalgae …

Magnetically Treated Water: Enhancing Stocking Density, Water Quality, And Growth In Nile Tilapia (Oreochromis Niloticus), Mortada M. A. Hussein, Ayatollah A. H. Abd-El Said, Asmaa N. Mohammed, Fatma M. M. Korni

Journal of King Abdulaziz University: Marine Science

This study aimed to explore the potential of using magnetic treated water (MTW) to increase stocking densities of growing Nile tilapia, Oreochromis niloticus, in storage, productive, and/or rearing holding facilities with limited mortality and water loss. The experimental period was 12 weeks, with 1500 O. niloticus fingerlings acclimated with maintained water parameters. After acclimation, 1350 were randomly selected and used for experiment setting up, including its triplicates. For each replicate, 225 fingerlings were distributed in three experimental tanks of MTW units as group set A, B and C, each received 50, 75, and 100 fingerlings, respectively. Besides, 225 fingerlings …

Environmental Assessment Of The Shatt Al-Basra Canal: Petroleum Hydrocarbons, Heavy Metals And Water Quality, Mohammed A. Jasim Aldoghachi, Bassam A. B. Alabdul Aziz, Shurooq Al-Mosawi

Journal of King Abdulaziz University: Marine Science

The environmental assessment of the Shatt al-Basra Canal in the area of the regulatory was studied. Petroleum hydrocarbons and heavy elements (cobalt (Co), zinc (Zn), lead (Pb), cadmium (Cd), and chromium (Cr)) in the waters of this area were analyzed. In addition, some water quality characteristics were evaluated (salinity, total dissolved solids (TDS), pH, oxygen, biological oxygen demand (BOD₅), chemical oxygen demand (COD), nitrate (NO_3^-), nitrite (NO_2^-), phosphate (PO₄ ^-3), and ammonia (NH₃). Petroleum hydrocarbons had been recorded in studied area and dominated with medium-chain alkanes, namely decane …

Impacts Of Coastal Development And Tourism On Fisheries And Aquatic Biodiversity Of The Red Sea: A Review, Lafi S. Al Solami

Journal of King Abdulaziz University: Marine Science

The Red Sea boasts a diverse coastal environment, unique coral reef biodiversity, and fisheries that support rapidly growing marine tourism. Its excellent tourism facilities make it a popular destination. However, intensive tourist activities such as swimming, boating, diving, and fishing have a negative impact on the coastal and marine ecosystem. Additionally, in line with Vision 2030, Saudi Arabia has launched mega projects along the Red Sea coast, increasing tourist activity and leading to human intrusion into critical yet vulnerable marine ecosystems like coral reefs, fish, sea grass beds, and mangrove stands. With the rise in tourism activities and disruptions to …

Microplastics Contamination In Marine Environment: Sources And Management Strategies, Aishah Hamdi, Mohammad Oves, Naser Alkenani, Majed Alshaeri

Journal of King Abdulaziz University: Marine Science

Microplastics (MPs) have been identified as emerging global pollutants because of their persistence, mobility, and significant ecological impacts. These particles, which result from the degradation of plastic waste and from various industrial and consumer activities, are now widespread in marine environments. The capacity of MPs to adsorb contaminants and bioaccumulate within food webs increases their ecological and health risks, prompting substantial concern regarding their long-term consequences for marine ecosystems and human health. This review synthesizes current knowledge regarding the composition, sources, transport, mechanisms, and environmental behavior of microplastics (MPs), with particular emphasis on marine systems. In these environments, the persistence …

Liver Function Test (Lfts) Revealed Ameliorative Effect Of Probiotics And Antibiotics As Therapeutic Agents Against Experimentally Induced Enterotoxemia In Goat, Muhammad Mohsen, Jam Kashif Zaman, Amjad Hussain Mirani, Nazeer Hussain Kalhoro, Benazir Sahito

Journal of Bioresource Management

Present study was conducted to evaluate the various liver enzymes in experimentally induced enterotoxemic goat inorder to ensure the therapeutic potential of probiotics and antibiotics. Experiment was conducted at Department of Veterinary Medicine, Sindh Agriculture University Tandojam in which 35 non-descriptive goats were housed and experimenatally induced with enterotoxemia by inoculation of Clostridium perfringens culture surgically by performing laparotomy into intestine. After apperence of positive clinical signs of infection, goats were randomly divided into 5 treatment groups i.e. Group 1 (negative control), Group 2 (positive control), Group 3 (yogurt, 5 % solution, 50 mL/time), Group 4 (Yeast, 5 % solution, …

Metabolic Markers And Gut Microbiota In Big Cats: A Relationship Between Diet, Health And Environment, Javeria Saman, Saima Sharif, Bushra Nisar, Shagufta Naz

Journal of Bioresource Management

The wild animals that occupy large territories were found to adapt less easily to captive environments compared to those with small home ranges. Among big cats, captivity posed a range of challenges, with stress emerging as central factor influencing overall welfare. Transition into captive environment was shown to alter both behavior and physiological processes, largely due to changes in their natural habitat, environmental complexity and diet. Several health issues reported in big cats including alopecia, gastrointestinal issues, neuromuscular disorders such as hindquarter paralysis were associated with underlying metabolic dysregulation. Metabolic health was recognized as critical component of overall well-being, closely …

Modeling Framework To Demonstrate Elimination Of A Vector Population: Tsetse Elimination In Chad, John Hargrove, Mahamat Hissene Mahamat, Moukhtar Aldjibert, Wilfrid Yoni, Djoukzoumka Signaboubo, Justin Darnas, Ernest Salou, Inaki Tirados, Albert Mugenyi, Priscille Barreaux, Philippe Solano, Antoine Marc Gaby Barreaux

All Peer-Reviewed Publications

Every year, over 700,000 people, particularly children under five, die from vector-borne diseases worldwide. Effectively controlling endemics and preventing new outbreaks requires an integrated approach that can lead to the elimination of both vectors and diseases. In the last two decades, integrating medical interventions and vector control has significantly reduced the incidence of Gambian Human African Trypanosomiasis (g-HAT), with the World Health Organization validating eight countries as having eliminated the disease as a public health problem. However, elimination of the tsetse vector has not been confirmed, leaving the possibility of re-emergence. We developed a six-step modeling framework to assess vector …

Haematological Responses Of Nile Tilapia (Oreochromis Niloticus) To Diets Supplemented With Varying Levels Of Plant Leaf Meals, Isaack V. Makombe, Harishchandra Pratap, Felister Urasa, Blandina R Lugendo

Tanzania Journal of Science

Plant leaf meals have the potential to replace fishmeal in fish feed formulation, hence reducing its cost and increasing fish production. This study evaluated the effects of plant leaf meals (PLMs) on haematological parameters in Nile tilapia (Oreochromis niloticus). The PLMs of cassava, sweet potatoes, cowpeas and common beans were substituted at the rate of 100% (mixed leaves of all four species, Diet 5), followed by each separate leaf meal at 100% (Diet 4), 75% (Diet 3), 50% (Diet 2) and 0% (control diet containing fish meal, Diet 1). Fish collected from Mbegani Fisheries Institute were kept in five, 80 …