Digital Commons Network^™

Breaking The Rules: Post-Translational Modifications And Proteome Dynamics During Anoxia, Chelsea Jordan Hughes

Dissertations and Theses

Most organisms are ill-equipped to survive anoxia, but some organisms have developed molecular strategies to mitigate the cellular stressors associated with a lack of oxygen. While it is established that anoxic survival is contingent on metabolic suppression, it is unclear what mechanisms support this change in gene expression. Gene expression is regulated by a variety of mechanisms within the cell, including alteration of chromatin structure through histone modifications and protein modifications which contribute to the ever-changing proteomic landscape of the cell. Protein modifications such as ubiquitylation can lead to the degradation of proteins, while modifications to transcription factors can change …

Sire Evaluations: Boer, Kiko, And Spanish Kid Weaning Weights In The Southeast, Alicia D. Aldridge

Tennessee State University Alumni Theses and Dissertations

Sire Evaluations: Boer, Kiko, And Spanish Kid Weaning Weights in the Southeast Research was completed with weaning weight ratio (WWR) and expected progeny difference (EPD) efficiency values compared from pedigree data across six seasons (2003-2009). Four models: WWR, 90- day unadjusted, adjusted, and new adjusted WW models compared sire breed, dam breed, month of birth, dam age, sex, litter size at birth, litter size at 90 day weaning age, and birth year effects. Correction factors were re-adjusted when non-genetic effects (sex, dam age, and litter size at birth and weaning) remained significant (p<0.05) in the original 90-day adjusted WW models. The new correction factors eliminated non-genetic effects (p>0.05). Sire breed effects were not significant …

Soybean Embryogenic Cell Culture Genetically Engineered, Gabriel Carrasquillo

Tennessee State University Alumni Theses and Dissertations

The inflation of population in the world has led to a high and consistent food demand which has reinvigorated worldwide interest to create high-yielding varieties of legumes. Soybean Glycine max (L.) Merrill, a member of this legume family has become one of the most essential food crops in the United States as well as in the world due its edible oil and protein content up to 20% and 54%, respectively. Because of its high importance as a food crop, major objectives in soybean breeding, improvement of protein and oil production need to be achieved. These objectives can be addressed using …

Isolating Novel Enzymes And Microorganisms For Use In Food Waste Fermentation, Shannon Marie Jeffries

Tennessee State University Alumni Theses and Dissertations

In the United States, upwards of 2 billion pounds of food waste are discarded yearly, an amount equivalent to $161 billion or 30-40% of all food production, making up the largest portion of landfill waste (Buzby, 2022). A small amount of food scraps are recycled through compost and re-purposed into animal feed, but these processes are costly, have minimal benefits, and have limitations (EPA, 2024). One of the most promising routes is converting food waste into value added products through microbial fermentation. Food waste is rich in carbohydrates, proteins, lipids, vitamins, and other trace minerals, making it the ideal environment …

Seed Careers App Impact On Nextgen Scholars At Tennessee State University, Jazmine Norwood

Tennessee State University Alumni Theses and Dissertations

The underrepresentation of minority groups in food, agriculture, natural resources, and human sciences (FANHS) careers remains a challenge, limiting diversity and growth within the industry. Agriculture degrees have been disproportionately awarded to White students, while Hispanic, Black, and Native American students receive significantly fewer degrees. Research suggests that financial assistance, experiential learning, and career outreach improve minority student retention and success in FANHS. To address these challenges, the USDA’s NextGen program awarded $18.1 million to Tennessee State University (TSU) to support underserved students through scholarships, internships, and career development initiatives, including the creation of the Students Exploring Employment and Development …

Application Of High-Pressure Processing And Real-Time Pcr For Preventing Foodborne Bacterial Diseases, Ranju Kafle

Tennessee State University Alumni Theses and Dissertations

Foodborne pathogens pose significant risks to public health and food safety, necessitating innovative control strategies. High-pressure processing (HPP) was evaluated as an intervention against Listeria monocytogenes in cold-smoked rainbow trout, with treatments at 500 MPa and 4.4 °C and 60.0 °C achieving up to a 5.03 log CFU/g reduction. The efficacy of nisin in suppressing Listeria monocytogenes and Listeria innocua during refrigerated storage was also examined, demonstrating significant microbial inhibition, particularly in the early storage phase, supporting its application as a hurdle technology. Additionally, the synergistic effects of HPP (400 MPa, 4 °C) and natural bioactive compounds, including lactic acid, …

Mutation Of Smarca4 Induces Cancer Cell-Intrinsic Defects In The Enhancer Landscape And Resistance To Immunotherapy, Yawen Wang, Ismail M Meraz, Md Qudratullah, Sasikumar Kotagiri, Yanyan Han, Yuanxin Xi, Jing Wang, Kadir C Akdemir, Jack A Roth, Yonathan Lissanu

Faculty, Staff and Student Publications

Cancer genomic studies have identified frequent alterations in genes encoding components of the SWI/SNF chromatin remodeling complex, including SMARCA4 and ARID1A. Importantly, clinical reports indicate that SMARCA4-mutant lung cancers respond poorly to immunotherapy and have dismal prognosis. In this study, we corroborated the clinical findings by using immune-humanized, syngeneic, and genetically engineered mouse models of lung cancer harboring SMARCA4 deficiency. Specifically, models with SMARCA4 loss showed decreased response to anti-PD-1 immunotherapy associated with significantly reduced infiltration of dendritic cells and CD4+ T cells into the tumor microenvironment. SMARCA4 loss in tumor cells led to profound downregulation of STING1, IL1β, and …

Exploring Soft Polymeric Nanoparticles For Targeted Biomolecule Delivery In Plant Cells, Mohammad Rashid

Theses and Dissertations

Safe and efficient agricultural practices require innovative technologies. Nanotechnology offers promising solutions for advancing plant agriculture by enabling the targeted delivery of biomolecules such as agrochemicals and nucleic acids. This study focuses on developing a safe nanotechnological approach for delivering molecules of interest into plant cells. We investigated the ability of four types of polymeric nanoparticles such as Polyethylene glycol N, Nʹ-dibutylethylenediamine (PEG-DB), Polyethylene glycol 2-pyrrolidin-1-yl-ethyl-amine (PEG-PY), Polyethyleneimine (PEI), Arabinoxylan (AX)+, and Zein+ to form conjugates with plasmid DNA (pDNA). Our findings confirm that plant-derived cationic nanopolymers, AX+ and Zein+, effectively bind negatively charged pDNA (pGFPGUSPlus) carrying a GFP reporter …

Suppression Of Brca1 Facilitates Kidney Regeneration, Kaira A Church, Xunian Zhou, Raghu Kalluri

Faculty, Staff and Student Publications

Maladaptive repair following kidney injury leads to the development of kidney disease. In this issue of JEM, Ajay et al. (https://doi.org/10.1084/jem.20231107) uncover the role of breast cancer susceptibility gene 1 (BRCA1) in cell cycle arrest, DNA damage, and cell senescence, preventing maladaptive repair.

Drosha: A New Tumor Suppressor In Pineoblastoma, Zhixuan Huang, Xueli Ren, Jian Hu

Faculty, Staff and Student Publications

In this Outlook, Huang et al. discuss a study in this issue of Genes & Development by Fraire et al. that shows that a deficiency in miRNA processors Drosha and Dicer and consequent cell cycle gene derepression promote pineoblastoma development. The authors highlight the heterogeneity of pineoblastoma's pathogenic mechanisms and its implications for therapeutic interventions in the clinic.

Switching On The Evolutionary Potential Of Pancreatic Cancer: The Tumor Suppressor Functions Of Pbrm1, Luigi Perelli, Giannicola Genovese

Faculty, Staff and Student Publications

Cell plasticity is a hallmark of cancer, enabling tumor cells to acquire multiple phenotypes responsible for tumor progression, metastasis, and therapy resistance. In this issue of the JCI, Kawai and colleagues leveraged genetically engineered mouse models (GEMM) of pancreatic ductal adenocarcinoma (PDAC) to demonstrate that loss of Pbrm1, a member of the SWI/SNF complex, drives dedifferentiation and aggressive tumor features. Pbrm1 loss activated a program of epithelial-to-mesenchymal transition (EMT) and allowed the emergence of poorly differentiated histologies that are commonly associated with high recurrence rate and dismal prognosis. These findings reveal the role of the SWI/SNF complex during PDAC evolution …

Hyaluronan Network Remodeling By Zeb1 And Itih2 Enhances The Motility And Invasiveness Of Cancer Cells, Sieun Lee, Jihye Park, Seongran Cho, Eun Ju Kim, Seonyeong Oh, Younseo Lee, Sungsoo Park, Keunsoo Kang, Dong Hoon Shin, Song Yi Ko, Jonathan M Kurie, Young-Ho Ahn

Faculty, Staff and Student Publications

Hyaluronan (HA) in the extracellular matrix promotes epithelial-mesenchymal transition (EMT) and metastasis; however, the mechanism by which the HA network constructed by cancer cells regulates cancer progression and metastasis in the tumor microenvironment (TME) remains largely unknown. In this study, inter-α-trypsin inhibitor heavy chain 2 (ITIH2), an HA-binding protein, was confirmed to be secreted from mesenchymal-like lung cancer cells when cocultured with cancer-associated fibroblasts. ITIH2 expression is transcriptionally upregulated by the EMT-inducing transcription factor ZEB1, along with HA synthase 2 (HAS2), which positively correlates with ZEB1 expression. Depletion of ITIH2 and HAS2 reduced HA matrix formation and the migration and …

A Comprehensive, Multi-Center, Immunogenomic Analysis Of Melanoma Brain Metastases, Lucy Boyce Kennedy, Amanda E D Van Swearingen, Marissa R Lee, Layne W Rogers, Alexander B Sibley, Jeff Sheng, Dadong Zhang, Xiaodi Qin, Eric S Lipp, Swaminathan Kumar, Aron Joon, Pixu Shi, Michael A Davies, Kouros Owzar, Carey K Anders, April K S Salama

Faculty, Staff and Student Publications

Background: Melanoma brain metastases (MBM) have a unique molecular profile compared to extracranial metastases (ECM). Description of the biological features and clinical outcomes of MBM will facilitate the design of rational therapies.

Methods: We examined the mutational landscape and gene expression profiles of MBM (74 patients) and ECM (34 patients) in paired patient samples from a previously published dataset with whole-exome sequencing (WES) and RNA sequencing (RNAseq) data from MD Anderson Cancer Center (MDACC). We also present findings from MBM from a new cohort of 14 patients from Duke University to strengthen investigation of somatic mutations and gene expression profiles. …

Harnessing Electrophiles In Vivo: A Pilot Study In Swine Using A Hydrophobic Radiopaque Formulation Of 2-Propylpentanoyl Chloride, Erik Cressman, Danielle Stolley, Natalie Fowlkes, Edd Felix, Waldemar Priebe, Steve Parrish, David Fuentes

Faculty, Staff and Student Publications

Manipulating biology through chemistry is older than the discipline of chemistry itself. Traditionally, selectivity of oral or intravenous drugs relied on preferential drug-receptor binding. A novel approach exploiting image-guided techniques to impart spatial selectivity opens up a wide range of new possibilities for study and manipulation of biology. Motivated initially by the poor prognosis for solid tumors such as liver cancer, we demonstrate the use of an extreme form of in vivo chemistry for targeted delivery of 2-propylpentanoyl chloride in a swine model. The ensuing reaction in tissue devitalizes it by multiple mechanisms with lasting effects and, critically, demonstrates very …

Judgment Bias, Fear, And Stress Responses Of Red Junglefowl And Athens Canadian Random Bred Chickens, Victor J. Oyeniran, Rosemary H. Whittle, Sara K. Orlowski, Shawna L. Weimer

Poultry Science Faculty Publications and Presentations

Understanding how poultry perceive and interpret their environment is essential to enhancing their welfare. Animal welfare science relies on measures of the behavioral and physiological components of affective states (positive and negative) as welfare indicators. There has been growing interest in using the judgment bias test (JBT) to study birds' affective states by assessing their responses to ambiguous cues. The objective of this study was to investigate the affective state of two chicken breeds with different evolutionary histories: the Red Junglefowl (RJF), the primary ancestor of modern chickens, and the Athens Canadian Random Bred (ACRB), a rustic domesticated breed, using …

Present And Future Of Microbiome-Targeting Therapeutics, Lauren E Lynch, Rachel Lahowetz, Christian Maresso, Austen Terwilliger, Jason Pizzini, Valeria Melendez Hebib, Robert A Britton, Anthony W Maresso, Geoffrey A Preidis

Children’s Nutrition Research Center Staff Publications

A large body of evidence suggests that single- and multiple-strain probiotics and synbiotics could have roles in the management of specific gastrointestinal disorders. However, ongoing concerns regarding the quality and heterogeneity of the clinical data, safety in vulnerable populations, and the lack of regulation of products containing live microbes are barriers to widespread clinical use. Safety and regulatory issues must be addressed and new technologies considered. One alternative future strategy is the use of synthetic bacterial communities, defined as manually assembled consortia of two or more bacteria originally derived from the human gastrointestinal tract. Synthetic bacterial communities can model functional, …

United Seas For Resilient Reefs: A Blueprint For Coral Conservation Through International Cooperation, John J. Bohorquez, Maoz Fine, Dana I. Grieco, David A. Gill, Ali Al-Swalimih, Jacqueline Padilla-Gamino, Isaiah W. Bolden, Margaux Hein, Callum Backstrom, Melanie Mcfield, Raphael Martinez, Benjamin Midberry, Fahd Al-Guthmy, Ellen K. Pikitch, Natalia Benejam, Chen Azulay, Britt Ronen, Oren Levy, Anne Mcelroy, Karine Kleinhaus

School of Marine & Atmospheric Sciences Faculty Publications

Coral reefs are one of Earth’s most vital and threatened ecosystems. They protect coastlines, generate billions of dollars of tourism revenue, support valuable fisheries, and contribute to the development of vital medicines. Yet half of the world's coral reefs have been lost in the last 50 years, and 90% of remaining reefs are projected to die off by the middle of this century, mainly due to warming seas from climate change.

Despite these challenges there is reason for hope. Some coral reefs – such as in the northern Red Sea and Gulf of Aqaba – have demonstrated remarkable resilience to …

Effects Of Spirulina And Nannochloropsis Supplementations On The Meat Quality And Sensory Properties Of Broiler Carcasses, Shreyash S. Patel

Master's Theses

Microalgae are a promising feed ingredient due to their high content of proteins, lipids, polysaccharides, vitamins, pigments, and enzymes. This study evaluated the effects of algae as a dietary supplementation on the appearance, quality, and sensory attributes of broiler carcasses. In each of experiment I and II, a total of 54 Ross-708 broilers were randomly obtained from the pool of 270 broilers in the 18 floor pens of soybean-mealbased diets at 0, 2.5%, and 5% Spirulina (Experiment I) and Nannochloropsis (Experiment II) supplements in the Poultry Unit at California Polytechnic State University (Cal Poly, San Luis Obispo, CA). Broiler carcasses …

Art, Play, And Neuroplasticity: Advancing Dementia Care Through Art-Enriched Environments, Piper Hutson, James Hutson

Faculty Scholarship

Dementia represents a global public health crisis, characterized not only by progressive memory loss but by a pervasive decline in cognitive flexibility, social functioning, and overall quality of life. Traditional therapeutic modalities, often limited to pharmacological management and cognitive training, have yielded only modest gains in symptom mitigation and patient well-being. Recent advances in neuroscience have illuminated the brain‘s capacity for neuroplasticity—its inherent ability to adapt, reorganize, and form new neural connections even in advanced age and the presence of neurodegenerative conditions. This emerging understanding invites a critical re-evaluation of dementia care strategies, highlighting the potential of non-pharmacological interventions to …

An Unusual Trumpeter Swan (Cygnus Buccinator) In Nebraska: Albinism Or Leucism?, Jacob C. Cooper, Peggy M. Huss, Eugene A. Huryn, Cody Mcgregor

Nebraska Bird Review

On 26 January 2025, we observed a large mixed flock of Trumpeter Swans (Cygnus buccinator) and Tundra Swans (C. columbianus) at Hansen Reservoir ca. 7 miles (11 km) NNW of North Platte along the east side of Nebraska Highway 97. The flock of 158 swans consisted of 4 “Whistling” Tundra Swans (C. c. columbianus), 153 typical Trumpeter Swans, and one individual that we were not immediately able to identify. Our unknown swan was in the company of a family group of Trumpeter Swans but immediately stood out due to its broad yellow base of …

Nebraska Ornithologists' Union Tri-State Meeting With Iowa Ornithologists' Union And South Dakota Ornithologists' Union, South Sioux City, Nebraska, May 16-18, 2025

Nebraska Bird Review

The NOU Spring Meeting was held at the Salvation Army Western Plains Camp in South Sioux City, Nebraska, in conjunction with the IOU and SDOU, with approximately 180 attending. The organizers of the meeting included Chris Anderson and Mark Schmidtbauer from South Dakota, Tyler Harms and Kevin Murphy from Iowa, and Ruth Stearns, Joel Jorgenson, and Paul Pearson from Nebraska.

A scientific poster session featuring the work of 9 ornithological researchers from UN-Kearney, USD-Vermillion, and Hitchcock Nature Center was held Friday evening. Thanks to Dr. Jacob Cooper from UNK, Dr. David Swanson from USD, and the students who participated.

Field …

Nou Tri-State Meeting—South Sioux City [Spring 2025 Nou Meeting Species Tally]

Nebraska Bird Review

Bird species tally (185) for the Spring 2025 NOU Tri-State Meeting, South Sioux City (Nebraska Ornithologists’ Union Tri-State Meeting with Iowa Ornithologists’ Union and South Dakota Ornithologists’ Union, South Sioux City, Nebraska, May 16–18, 2025)

The Mayfly Newsletter, Donna Giberson

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Abstracts Of Posters Presented At South Sioux City Nou Meeting

Nebraska Bird Review

A scientific poster session featuring the work of 9 ornithological researchers from UN-Kearney, USD-Vermillion and Hitchcock Nature Center was held Friday evening. Thanks to Dr. Jacob Cooper from UNK, Dr. David Swanson from USD, and the students who participated.

Wandering from the River: On- and Off-River Abundance of Interior Least Terns along the 59-Mile Reach of the Missouri National Recreational River. Zoe Donelan, Hannah Slattery, Kyle Jorgensen, and David L. Swanson, University of South Dakota, Department of Biology.

Preliminary Results of Raptor Migration Monitoring at Hitchcock Nature Center. Kellie Hayden, Jerry Toll, and Sandy Reinken, Hitchcock Nature Center, Pottawattamie Conservation, …

Spring Field Report, March–May 2025, W. Ross Silcock

Nebraska Bird Review

Perhaps the most important takeaway this spring is the continuing phenomenon of large numbers of species with early spring arrival dates. Although about half as many as 2024, there were 64 examples of either tied or new early arrival dates, most obviously of insectivores such as swifts, flycatchers, vireos, swallows, pipits, longspurs, and 10 warbler species. Among these, record or near-record early were Whip-poor-will, Black-necked Stilt, Marbled Godwit, Black Tern, Olive-sided Flycatcher, Purple Martin, Rock Wren, Gray Catbird in central, Spotted Towhee in north and west, and Hooded Warbler.

Following along logically perhaps with early arrival dates were a number …

The Nebraska Bird Review (June 2025) 93(2), Whole Issue

Nebraska Bird Review

Spring Field Report, March–May 2025, by W. Ross Silcock

NOU Spring Field Days and Annual Meeting in South Sioux City, May 16–18, 2025

Abstracts of posters presented at Spring 2025 NOU Meeting

Spring 2025 NOU Meeting Species Tally

An Unusual Trumpeter Swan (Cygnus buccinator) in Nebraska: Albinism or Leucism? by Jacob C. Cooper, Peggy M. Huss, Eugene A. Huryn, and Cody McGregor

Subscription and Organization Information

Oviposition-Deterrent And Repellent Effects Of Synthetic Plant-Derived Compounds On The Pulse Beetle, Callosobruchus Chinensis (Coleoptera: Chrysomelidae: Bruchinae), Akki Suma, M Rajadurai, Ac Lokesh, Mb Anusha, S Krishna Kumar

The Philippine Agricultural Scientist

The pulse beetle, Callosobruchus chinensis (Linnaeus, 1758), is a major stored pest of pulses (Fabaceae) in India. While effective control of this widespread and destructive insect pest is essential, continuous insecticide use poses environmental concerns, thereby necessitating alternative, eco-friendly control methods. This study evaluated the oviposition-deterrent effects of 7 synthetic compounds (linalool, guaiene, citral, estragole, 4-nonanone, alloaromadendrene, and citronellal) on C. chinensis. Linalool showed the strongest suppression of egg-laying at higher concentrations, with the lowest egg count at 500 ppm compared to the control, followed by citral, citronellal, and guaiene. Linalool was also effective in suppressing egg hatching at …

Effects Of Probiotic Supplementation On Growth Performance, Nutrient Digestibility, And Enteric Methane Emission Of Growing Dairy Buffaloes (Bubalus Bubalis L.), Christian Lualhati, Karl Reginald Managa, Charity Castillo, Phoebe Lyndia Llantada, Arnel Del Barrio

The Philippine Agricultural Scientist

This study assessed the impact of probiotics on the growth performance, nutrient digestibility, and enteric methane emission of 4 growing dairy buffaloes (Bubalus bubalis L.) with similar body weight, age, and sex. The animals were allocated into 4 treatments following a Latin Square design: control/no probiotic supplement (Trt 1); with 5 mL of probiotic supplement (Trt 2); with 10 mL of probiotic supplement (Trt 3); and with 15 mL of probiotic supplement (Trt 4). The growers were offered the dietary treatments at the start of the adaptation phase up to the end of the experimental phase per period. Animals …

Isolation And Enumeration Of Salmonella Enterica From Fresh Lettuce (Lactuca Sativa L.) Leaves And Its Susceptibility To Different Washing Protocols, Meinard Talplacido, Angella Melissa Carlos, Bryan Rey Oliveros, Ida Dalmacio

The Philippine Agricultural Scientist

Lettuce is one of the most consumed fresh produce items in the country, but it also serves as a vehicle for human pathogens, posing a risk of foodborne illness. This study used conventional microbiological and molecular analyses to obtain the concentration and prevalence of Salmonella enterica in fresh Green Ice and Iceberg lettuce leaves from selected supermarkets in Los Baños, Laguna, Philippines. The efficacy of different household washing procedures in reducing S. enterica on lettuce leaves was also investigated. Of the 19 composite Green Ice lettuce and 21 composite Iceberg lettuce samples tested, 5.26% and 4.76%, respectively, were contaminated with …

Effects Of Water Deficit Stress And Mycorrhiza, Azotobacter Chroococcum, And Azospirillum Lipoferum Biofertilizers On Yield And Morphological Traits Of Wheat (Triticum Aestivum L.) Cultivars, Mehdi Rashnou, Farshid Aref, Bahram Amiri, Masoud Rafiee, Mahdi Zare

The Philippine Agricultural Scientist

Crop production in various parts of Iran faces significant constraints due to insufficient water availability, which is a major challenge to agricultural productivity. To study the effects of water deficit stress on shoot and root growth characteristics and seed yield of different wheat (Triticum aestivum L.) cultivars, an experiment was conducted using a randomized complete block design with a split-plot arrangement and 3 replications. The main factor was irrigation treatments (full irrigation during the growing season [S1], water withholding after flowering [S2], and water withholding after grain filling at the milk stage [S3]). The secondary factors were wheat cultivars …