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Management-Driven Shifts In The Bison Fecal Microbiome And Its Relationship To Stec Prevalence, Ritesh Ray

Graduate Research Theses & Dissertations

Contamination of meat samples from pathogens such as Shiga toxin-producing E. coli (STEC), especially in ruminants, is an ever-growing issue for the safety of global meat production. Recent studies have reported STEC in the livestock and food industry with a limited focus on wildlife. Although cattle serve as the primary reservoir for STEC, American bison (Bison bison bison) is the largest ruminant inhabiting North America, sharing similar genetics, and grazing habits, making them an ideal alternative STEC reservoir. Re-introduction for prairie conservation and meat farming has brought bison herds closer to anthropic environments than before. Recently bison meat has become …

Characterization Of The Effects Of Atp On Primary Brainstem Glial Cells, Donovan J. Aardema Faigh

Graduate Theses/Dissertations

Migraine and temporomandibular joint disorder (TMD) are debilitating orofacial pain disorders that negatively impact one’s quality of life. These disorders are characterized by elevated levels of adenosine triphosphate (ATP) and activation of central nervous system glial cells that promote and maintain central sensitization and enhance pain signaling in response to inflammatory stimuli. The goal of my study was to characterize the effects of the pro-inflammatory signaling molecule adenosine triphosphate (ATP) on intracellular calcium levels, mRNA levels, and protein expression in astrocytes, microglia, and oligodendrocytes to better understand the role of ATP in promoting central sensitization. To address this goal, primary …

Genomic Signatures Of Decline And Recovery In An Endangered Bat, Myotis Grisescens, Marxe Altman-Orbach

Graduate Theses/Dissertations

Tremendous population declines may result in the loss of genetic diversity, which can, in turn, lead to reduced adaptive potential, overexpression of deleterious phenotypes, and further population declines. In this way, genetic drift from a severe demographic decline may ultimately lead to an extinction vortex. However, populations that avoid the loss of genetic diversity may remain viable, despite demographic crashes. Therefore, understanding genetic diversity is vital for assessing the conservation status of species that have undergone declines, and can even reflect the effectiveness of management actions. I investigated signatures of genetic bottlenecks in the gray bat (Myotis grisescens), …

United States Mainstream Meat Market Entry: An Analysis On Speciality Goat Meat Products, Madison Raye Schmitz

Graduate Theses/Dissertations

Understanding consumers' perspectives and motivations is key when looking into making goat meat more accepted in the mainstream meat market. Previous research has shown there is a demand for goat meat from different populations in the US. Consumers also care more about the quality of the food they purchase. Understanding that goat meat has lower cholesterol and is leaner than other red meat is important to incorporating goat meat in niche consumer markets. Providing consumers with a product that is familiar to them is more acceptable than giving consumers a cut of meat they are unfamiliar with. Standard products like …

The Effect Of Sex On A-To-I Editing In Skeletal Muscle During Inflammation, Skyler Lee

Graduate Theses/Dissertations

Adenosine-to-inosine (A-to-I) RNA editing is a post-transcriptional mechanism mediated by ADAR enzymes that can alter RNA sequence, protein function, and gene regulation. Because ADAR1 is upregulated during inflammation and plays a critical role in innate immunity, previous studies investigated the effects of acute inflammation on RNA editing in male and female mice. Lipopolysaccharide (LPS) was used to induce inflammation, and RNA editing levels were examined in the heart, brain, and skeletal muscle. While editing levels in the heart and brain remained unchanged, skeletal muscle exhibited tissue- and sex-specific differences in editing. In this thesis, LPS treatment affected selective editing, increasing …

If You Build It, Will They Stay? Fish Communities Continue To Utilize Restored Habitat A Decade Following An Instream Restoration, Alexis L. Vandenberg

Masters Theses

Lotic ecosystems are degraded at an alarming rate due to anthropogenic activities. In order to combat these changes, stream restorations are implemented to improve conditions such as water quality, habitat heterogeneity, and bank stability. However, the impacts of these restorations are often understudied and long-term impacts are unknown. Restorations are typically designed to be ‘fish attractors’; this idea is known as the ‘Field of Dreams hypothesis’ – ‘if you build it, they will come’. This study uses data collected in Kickapoo Creek, Coles County from 2009 to 2015 and again in 2021 and 2022 to assess the long-term impacts of …

Compositional And Functional Shifts In Fish Community Structure In Response To Low-Head Dam Removals, Joshua D. Bruegge

Masters Theses

Low-head dams serve as intermittent barriers to aquatic connectivity and directly alter both upstream and downstream habitats and biotic communities. In 2018 and 2019, two low-head dams in the Vermilion River watershed of eastern Illinois were removed, reconnecting over 1,700 kilometers of upstream habitat within the basin. To determine the impacts of these dam removals, fish community surveys were conducted at 12 fixed sites across three reaches in two rivers from 2012-2022. This study assessed reach-specific temporal responses of the fish community and habitat characteristics. I linked trait expressions within the fish community to abiotic variables using the co-inertia RLQ …

Signs Of Life: Interkingdom Communication And The Sympoietic Organism, Scott F. Gilbert

Biology Faculty Works

No abstract provided.

Beyond The Square Pulse: Waveform Shape, Eeg Correlates, And The Pursuit Of Natural Sensation In Tens, Jason Whitson

Honors Undergraduate Theses

This study investigates the relationship between electrical stimulus characteristics of shape and charge on evoked sensations and electroencephalogram (EEG) data during multi-waveform transcutaneous electrical nerve stimulation (TENS) of the median nerve. Neuromodulation methods have traditionally had little control over the location and quality of their associated evoked sensation (e.g., electric, vibration, touch). This experiment utilized five unique stimulus waveforms during TENS stimulation. EEG data were collected concurrently to provide an introductory objective measure of the neural responses underlying these sensory changes. Eleven participants completed three tasks (thresholding, super-threshold stimulation, two-alternative forced choice) using a two-electrode TENS approach. Stimulus waveforms were …

Complexity Theory And Environmental Conservation Management: A Case Study With Coyote And Red Wolf Hybridization, Gretchen E. Mahalic

Honors Undergraduate Theses

Conservation biology and invasion biology are value-led scientific disciplines founded on environmental advocacy, operating at the intersection of research and action. Though these disciplines are known for their scientific veracity, unexamined values are often promulgated without scrutiny and drive decisions that work against good ecological outcomes. A case in point is that coyotes migrating into southeastern territory are framed as the greatest threat to the conservation of the endangered red wolf because hybridization is seen as diminishing their species integrity. Applying Val Plumwood’s ecofeminist account of dualistic frameworks, I will use this case to show how the vilification of the …

Establishment Of An In Vitro Dss-Induced Epithelial Injury Model To Investigate Il-6 Signaling And Microbial Metabolite Mediated Barrier Recovery, Srijal Kunwar

Graduate Studies Theses and Dissertations 2026

Dextran sodium sulphate (DSS) is widely used in vivo to induce epithelial injury; however, the molecular effects of DSS on intestinal epithelial cells, that investigate inflammatory and metabolic repair mechanisms remain unclear. Here, we establish an in vitro DSS-induced epithelial damage model using differentiated Caco-2 cell monolayer. Exposure to 2.5%(w/v) DSS for 24 hours resulted in significant upregulation of pore forming protein Claudin-2(2.677-fold) and oxidative marker, NOX-1(1.866-fold), accompanied by increased ROS production confirmed via DHE staining. Increased epithelial permeability was observed by TEER analysis, without significant cytotoxicity. Surprisingly, ELISA analysis revealed significant downregulation of IL-6 (p-value …

24-H Circadian Rest-Activity Rhythms And Physical Frailty In Older Adults: A Biopsychosocial Perspective On Sex Differences And Social Capital, Jisu Kim

Theses and Dissertations

Physical frailty is a prevalent geriatric syndrome characterized by age-related decline across multiple physiological systems. Physical frailty is associated with an elevated risk of adverse health outcomes, including chronic disease progression and all-cause mortality, representing a significant and growing public health concern. Disrupted circadian rest-activity rhythms (RARs) are increasingly recognized as clinically meaningful markers of physical frailty in older adults. However, the extent to which biological sex and social determinants of health (SDoH), particularly social capital, contribute to or modify the associations between RARs and physical frailty remains underexplored.

Therefore, this dissertation summarizes the existing literature on age-related changes in …

Climate, Biogeography, And Dispersal: A Unifying Framework For Ameri-Caribbean Dry Forests, Pablo Lopez Bustamante

Theses and Dissertations

Seed dispersal is a crucial ecological function that enables plants to alter their distributional range, avoid intense competition and natural enemies near the parent plant, and recover from disturbances. These processes shape species and functional composition of forests. Wind has traditionally been considered the dominant dispersal mechanism in Ameri-Caribbean dry forests, yet growing evidence suggests that other dispersal mechanisms also play significant roles in this ecosystem, responding differently to biotic and abiotic conditions. Still, how dispersal syndrome composition influences plant communities, the ecological drivers underlying these patterns, and the effects of climate change on dispersal syndrome dominance remain poorly understood. …

Sex In Plants: Birth, Death, And Trying To Survive In Changing Climates, Elena M. Meyer

Theses and Dissertations

Plant mating systems are key determinants of fitness. Angiosperms exhibit a wide variety of reproductive strategies, including self-fertilization (or selfing), outcrossing, and mixed mating. It has been estimated that up to 43% of plant species are capable of selfing, and selfing has been hypothesized to provide reproductive assurance where opportunities for mating may be limited, such as in marginal environments. However, questions regarding the overall distribution of selfing rates across angiosperms, the maintenance of mixed mating in the face of inbreeding depression, and the impact of self-fertilization on diversification rates remain incompletely resolved. Here, we explore the dynamics of self-fertilization …

Self-Movement Cue Ability As A Potential Moderator For The Relationship Between Binge Drinking And Posttraumatic Stress Disorder Symptoms, Dominoe Anna Jones

Graduate Research Theses & Dissertations

Alcohol use disorder (AUD) has been found to co-occur in a range of 30% to 50% of individuals diagnosed with posttraumatic stress disorder PTSD. The current study aimed to assess whether this high comorbidity could be partially explained through a shared association with poorer function and reduced volume in the hippocampus. Self-movement cue processing, a behavioral ability previously shown to be associated with hippocampal functioning, was measured through a dead reckoning task (DRT) to test this hypothesis. The current study failed to replicate previous findings between alcohol use and DRT and alcohol use and posttraumatic stress symptoms (PTSS). Performance on …

Nutritional Niche Separation Amongst Sympatric Threatened Lemurs: The Role Of Micronutrients In Food Selection And Implications For Physiological Health, Maire K. O'Malley

Graduate Research Theses & Dissertations

Madagascar’s biodiversity is heavily affected by anthropogenic change and consequently, conservation management plans need to reflect current research to protect biodiversity. Primate nutritional ecology offers valuable insights into ecology, evolution, health, habitat requirements, social behavior, and conservation management strategies. Macronutrients (fat, carbohydrates, and protein) provide consumers with energy whereas micronutrients (vitamins and minerals) are essential to metabolic processes but are frequently overlooked. This study provides a novel examination of dietary niche separation by comparing mineral consumption between three sympatric species differing in feeding strategies: 1) Grey Bamboo lemur (Hapalemur griseus), a bamboo specialist, 2) Diadema sifaka (Propithecus diadema), a folivore, …

Feasibility Of Corn Stover For Biogas Production In Northwestern Illinois: A Case Study Of Jo Daviess And Carroll Counties, Anthony Amotoe-Bondzie

Masters Theses

The growing demand for renewable energy and sustainable waste management has intensified interest in agricultural residues such as bioenergy feedstocks. This study evaluated the feasibility of converting corn stover into biogas through anaerobic digestion (AD) in Jo Daviess and Carroll Counties, Illinois, with consideration of the Savanna Industrial Park as a potential centralized processing hub. Although corn stover represents one of the largest biomass resources in the United States, this research identifies a critical gap between theoretical availability and practically recoverable feedstock. Using a mixed-methods framework, the study integrates biomass quantification, methane yield modeling, economic analysis, and policy assessment. Results …

Epigenetic Modifications In Dedifferentiated Liver Cells To Restore Lost Liver Function, Shreya Sharma

Masters Theses

The goal of the study is to investigate whether the epigenetic modifications can enhance the restoration of liver gene expression in dedifferentiated hepatoma cells or not. For the study, we used dedifferentiated H11 hepatoma cell lines which overexpress one of a panel of introduced candidate liver-enriched transcription factors H11-HNF1a, H11-CREB3L3, H11-CREG1, H11- HHEX, H11-HNF6, and H11-IGFBP1. The cells were treated with the DNA methyltransferase inhibitor 5-azacytidine and the histone deacetylase inhibitor sodium butyrate, both individually and in combination. Gene expression analysis was determined by RT-qPCR, which revealed that while transcription factor overexpression alone produced limited activation, epigenetic modulation, particularly Histone …

Layers Of Biodiversity: Linking Urban Garden Plants And Arthropods, Chenyang Su

Dartmouth College Ph.D Dissertations

Diverse arthropods play essential roles in urban agriculture, while urban agriculture provides food and habitat that support arthropod biodiversity in fragmented urban landscapes. In urban community gardens, a form of urban agriculture characterized by allotment-style gardens, arthropods interact with plants and green space across multiple spatial scales of management, from individual gardeners' plots to broad urban landscapes. Understanding how plant communities in urban community gardens and urban landscapes shape arthropod biodiversity and interactions across scales can inform both agricultural management and urban biodiversity conservation. In this thesis, I conducted a series of field studies on ecologically and economically important arthropods …

Impact Of Water Level Fluctuations On Fish Populations In Eastern South Dakota Rivers, Benjamin Schall

Electronic Theses and Dissertations

Riverine systems are highly dynamic, and changes in flow conditions can impact the population dynamics of fishes. Increased flows from historical averages in eastern South Dakota rivers have likely impacted fish populations. The goals of this study were to 1) better understand the changes in resource use by Blue Suckers Cycleptus elongatus through retrospective eye lens stable isotope analysis, 2) determine the effects of record flooding in the James River on complex woody habitat and Flathead Catfish Pylodictis olivaris population structure, and 3) assess the influence of river discharge and temperature on Channel Catfish Ictalurus punctatus and Flathead Catfish condition …

Notes On The Ichthyofauna Associated With Artisanal Fishing In A Severely Impacted Estuary In The Southwestern Gulf Of Mexico During The Rainy Season, Rodrigo Vivanco-Cadena, César Meiners-Mandujano, Roberto Martín Cruz-Castán

Gulf and Caribbean Research

No abstract provided.

Manatee Dock Sign Distribution And Its Influence On Publicly-Sourced Sighting Reports In Alabama, Daniela Payán Arias, Sophia C. Corde, Ruth H. Carmichael

Gulf and Caribbean Research

No abstract provided.

A Comparative Analysis Of The Ecophysiology Between The Invasive Macroalgae Starry Stonewort And The Native Macroalgae Chara To Predict Invasion Potential, Thomas J. Zellmer

All Graduate Theses, Dissertations, and Other Capstone Projects

Starry stonewort (Nitellopsis obtusa) is an invasive macroalga from Eurasia. Currently, starry stonewort’s invasive range consists of the upper Midwest and Great Lakes region, including the Mississippi River. Starry stonewort poses recreational and ecological problems by forming dense canopies that extend through the water column. Starry stonewort occupies a late-season niche space with dense biomass occurring in late July and persisting well into autumn. There is a lack of information on the ecophysiology of starry stonewort. In this study, two populations of starry stonewort, Minnesota and New York, were analyzed to compare regional differences in ecophysiology, along with chara (native …

A Complete Diploid Human Genome Benchmark For Personalized Genomics, Nancy F. Hansen, Nathan Dwarshuis, Hyun Joo Ji, Arang Rhie, Hailey Loucks, Glennis A. Logsdon, Mitchell R. Vallger, Jessica M. Storer, Juhyun Kim, Eleni Adam, Nicolas Alternose, Dmitry Antipov, Mobin Asri, Sofia Barreira, Stephanie C. Bohaczuk, Andrey V. Bzikadze, Sara A. Carioscia, Andrew Carroll, Kuan-Hao Chao, Yanan Chu, Arun Das, Peter Ebert, Adam English, Mark Fleharty, Laura E. Fleming, Giulio Formenti, Andrea Guarracino, Gabrielle A. Hartley, Katharine Jenike, Jenna Kalleberg, Yu Kang, Robert King, Josipa Lipovac, Mira Mastoras, Matthew W. Mitchell, Shloka Negi, Nathan D. Olson, Keisuke K. Oshima, Luis F. Paulin, Brandon D. Pickett, David Porubsky, Jane Ranchalis, Desh Ranjan, Mikko Rautiainen, Harold Riethman, Robert D. Schnabel, Fritz J. Sedlazeck, Kishwar Shafin, Mile Sikic, Steven J. Solar, Alexander P. Sweeten, Winston Timp, Justin Wagner, Dongahn Yoo, Ying Zhou, Erik Garrison, Evan E. Eichler, Michaeel C. Schatz, Andrew B. Stergachis, Rachel J. O'Neill, Karen H. Miga, Steven L. Salzberg, Sergey Koren, Justin M. Zook, Adam M. Phillippy

School of Medical Diagnostics & Translational Sciences Publications

Human genome sequencing typically relies on mapping reads to a reference genome to call variants, but this approach introduces technical biases, excluding duplicated and structurally polymorphic regions of the genome. To overcome this, we present a telomere-to-telomere genome benchmark with near-perfect accuracy across 99.4% of the diploid HG002 genome. This benchmark adds 701.4 Mb of autosomal sequence and both sex chromosomes (216.8 Mb), which were absent from prior benchmarks. We annotated genes and repeats on both haplotypes, including 19,956 protein-coding genes on the maternal haplotype and 19,190 on the paternal haplotype, and developed new methods to measure the accuracy of …

Evaluating Multi-Year Transferability Of Single- And Multi-Class Machine Learning For Remote Monitoring Of Beaver-Engineered Landscapes, Harris J. Sloan

Graduate Student Theses, Dissertations, & Professional Papers

Long-term satellite image archives enable ecological monitoring at large scales, but manual extraction of fine-grained features (e.g., beaver dams) remains time-consuming. Machine learning models may automate these tasks, but their temporal transferability for multiannual monitoring is still poorly resolved.

We evaluated the performance of two complementary neural-network frameworks across a 100,000-hectare watershed in Montana USA, using a decade of satellite imagery (2014-2024): (i) a single-class ResNet-50 beaver dam detector, and (ii) a multi-class ResU-Net landcover classifier.

In 2024, the dam detector identified 352 of 404 mapped dams, achieving balanced accuracy = 93.6%, precision = 79.8%, recall = 87.1%, F1 = …

The Importance Of Hippotherapy: Improving The Lives Of The Disabled Through Alternative Therapy, Jennifer N. Stapleton

UROP Posters

For centuries, horses have been suggested as “healers” and “helpers,” although it wasn’t until the 1900s that horseback riding was scientifically proven as a treatment and rehabilitation method. The exploitation of hippotherapy, the implementation of the three-dimensional pelvic movement stimulated by the oscillation and gait of the horse to combat symptoms of diseases and disorders, has since become a flourishing field of study, but one that is often overlooked. Benefits of hippotherapy often include increased motor neuron function, facilitating proper anatomical alignment, and reducing spasticity. This research analyzes the diverse and dynamic nature of treatment that hippotherapy facilitates, exploring the …

Determining Genes Involved In Recovery From Chemotherapy In 4t1, Emt6, And Eo771 Breast Cancer Cells, Akash Jagdeesh, Joseph Landry Ph.D.

UROP Posters

Breast cancer is one of the most common types of cancer, and often has poor prognosis. Traditional treatments for breast cancer include chemotherapy, which involves a chemical attack on all growing cells within the body. However, these treatments are not 100% effective, and patients might experience recurrence in their cancer months or years after achieving remission. Cancer cells can evade chemotherapy through five mechanisms: senescence, quiescence, cytoprotective autophagy, and apoptosis/necroptosis suppression. Prior studies have conducted CRISPR screens and experiments involving inhibition of epigenetic regulators, to alter the regulation of genes that can contribute to breast cancer chemotherapy resistance. This study …

Channel-Opening Kinetics Of Complex Kainate Receptors, Noah S. Saunders

Electronic Theses & Dissertations (2024 - present)

Ionotropic glutamate receptors (iGluRs) are found both pre- and postsynaptically in neuronal membranes, and mediate the majority of fast excitatory synaptic transmission in response to the binding of glutamate, a major excitatory neurotransmitter in the central nervous system (CNS). Ionotropic glutamate receptors are divided into three distinct subtypes: kainate, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), and N-methyl-D-aspartic acid (NMDA) receptors. Auxiliary proteins specific to each receptor subtype also play a role in tuning both receptor expression and gating properties, such as neuropilin and tolloid-like (NETO) proteins that modulate kainate receptors, and transmembrane AMPA receptor regulatory proteins (TARPs) that regulate AMPA receptors. Like …

Combating Antibiotic Resistance With Plasmid-Dependent Phages, Rhiannon T. Mariano

Electronic Theses & Dissertations (2024 - present)

The alarming rise of carbapenem-resistant bacteria poses an urgent global health threat, driven in large part by the horizontal spread of carbapenemase-encoding plasmids (Ma et al., 2023). These plasmids are often highly mobile and transmissible, enabling rapid dissemination of resistance genes across diverse bacterial populations. Their mobility is largely mediated by conjugation, a process dependent on the formation of pili, which serve as physical bridges for plasmid transfer between cells (Murray et al. 2022; San Millan and MacLean 2017; Virolle et al. 2020). Beyond their role in gene exchange, these pili also act as entry points for a unique class …

Fluorescent Protein Imaging Approaches Uncover Dynamic Subcellular Distribution Of Core Clock Proteins In Neurospora Crassa, Ziyan Wang

Dartmouth College Ph.D Dissertations

Circadian clocks are endogenous self-sustained timekeeping systems that allow organisms to anticipate and adapt to daily environmental cycles. Found in diverse forms of life, these molecular clocks regulate the expression of ~ 40% of the genome in animals, fungi, and plants. In animals and fungi, circadian oscillators are built on transcription-translation feedback loops (TTFLs), in which positive regulators drive the expression of negative regulators which, once synthesized and imported into the nucleus, inhibit their own activators. This precisely regulated negative feedback incorporates multiple steps of delays that are essential for generating ~24-hour rhythms. Through these mechanisms, circadian clocks temporally coordinate …