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Splenic Stromal Niches Support Hematopoiesis Of Dendritic-Like Cells From Precursors In Bone Marrow And Spleen., Pravin Periasamy, Jonathan Tan, Kristin Griffiths, Helen O'Neill
Splenic Stromal Niches Support Hematopoiesis Of Dendritic-Like Cells From Precursors In Bone Marrow And Spleen., Pravin Periasamy, Jonathan Tan, Kristin Griffiths, Helen O'Neill
Jonathan Tan
Objective The aims of this study are to test the ability of stromal cells from murine spleen to support hematopoiesis, to define the tissue source of precursors that seed these hematopoietic niches, and to determine the type of cells produced. Materials and Methods Cloned isolates of murine spleen stroma have been developed that support hematopoiesis. Analysis has been investigated in terms of tissue source of progenitors. Type and number of cells produced were analyzed by flow cytometry. Results Hematopoietic precursors that seed cocultures exist in spleen and bone marrow (BM), but not thymus. Cell production is highest if overlay cells …