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Food Of The Red Drum, Sciaenops Ocellata, From Mississippi Sound, Robin M. Overstreet, Richard W. Heard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Examined digestive tracts of the red drum in Mississippi Sound contained mostly decapod crustaceans. Crustaceans accounted for 34 of 59 encountered taxa, more than reported from any other region. Nevertheless, the general diet for 104 fish with food contents out of the 107 examined is similar to that reported for red drum in several other studies from other areas. In addition to crustaceans, fishes followed by polychaetes occurred as the most important items (in 99, 43, and 15% of the drum with food, respectively). Blue crabs occurred in even more drum than the frequently encountered penaeid shrimps. Other commercial species …

Suppressed Rejection Of Trichinella Spiralis In Immunized Rats Concurrently Infected With Eimeria Nieschulzi, Donald W. Duszynski, Deborah Russell, Shirley A. Roy, Gilbert A. Castro

Harold W. Manter Laboratory of Parasitology: Faculty Publications

This study deals with the effect of Eimeria nieschulzi infection on the host immune response to Trichinella spiralis. Six male Sprague-Dawley rats were inoculated with 10⁴ and six with 10⁶; sporulated E. nieschulzi oocysts. On days 2, 8, and 16 post-inoculation (PI), two rats from each infected group, and their paired uninfected controls, were killed and mucosal scrapings from their small intestines were assayed for peroxidase activity. Peroxidase levels were higher than corresponding control values on days 2 and 16 PI and significantly lower than the controls on day 8 PI. These data led us to …

Capillaria Sp. From Cryptotis Parva, Joseph N'Dong

Masters Theses

Twenty-nine Cryptotis parva were trapped at Lincoln Log Cabin State Park in Coles County Illinois between 22 December 1976 and 31 January 1978. Trapped specimens were necropsied and examined for the presence of helminth parasites. Nematode infections were found to be present in eight (27.2%) of the shrews collected with five (17.2%) of the males and three (10.3%) of the females infected. Three adult female nematodes were recovered from the small intestines of the host specimens, described and identified as the genus Capillaria. No male Capillaria were obtained and therefore species identification was impossible. However, the general appearance of …

Epizoic Protozoa Of Planktonic Copepoda And Cladocera From A Small Eutrophic Lake, And Their Possible Use As Indicators Of Organic Water Pollution, Michael Stevens Henebry

Masters Theses

The epizoic protozoa of planktonic copepoda and cladocera from a eutrophic lake (Ashmore Lake, Coles County, Illinois) were studied over a 13-week period in the summer and fall of 1975. All organisms were collected with a 125 micron mesh plankton net. Epizoans (all peritrichs and suctorians) were hosted by three of four species of copepods and four of the ten species of cladocerans. The percentage of total organisms hosting epizoans varied significantly (0.001 alpha level) by month, but averaged 11.6 percent. This high occurrence of epizoans was thought to be related to the highly-eutrophic conditions existing in the lake. If …

Poecilancistrium Caryophyllum And Other Trypanorhynch Cestode Plerocercoids From The Musculature Of Cynoscion Nebulosus And Other Sciaenid Fishes In The Gulf Of Mexico, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Examination of over 3,000 fish, all sciaenids, has shown that plerocercoids of Poecilancistrium caryophyllum infect Cynoscion nebulosus, Bairdiella chrysura, Sciaenops ocellata, C. arenarius, Micropogonias undulatus, and Pogonias cromis in Mississippi Sound. In addition to P. caryophyllum, a pseudophyllidean-like trypanorhynch also infected C. nebulosus and M. undulatus. Pseudogrillotia pleistacantha selectively infected large P. cromis, Pterobothrium heteracanthum infected M. undulatus, and Pterobothrium lintoni infected Menticirrhus americanus. Prevalence and intensity of infections of P. caryophyllum in C. nebulosus from Mississippi, roughly 40% of the fish each possessing an average of about two worms, …

Herpetomonas Megaseliae And Crithidia Harmosa: Growth On Blood-Agar Plates, Amy Doran Kepel, John J. Janovy Jr.

John J. Janovy, Jr. Publications

A technique for culturing two species of trypanosomatid flagellates on blood-agar plates is described. Herpetomonas megaseliae and Crithidia harmosa were grown as discrete clone colonies on plates prepared in sterile petri dishes with a medium derived from that of Tobie et al. (1950, J. Parasitol. 36: 48-54). Plates were inoculated with 0.01 ml flagellate suspension and the inoculum was spread evenly over the surface of the agar with a sterile glass spreader. Plates were incubated in a high humidity chamber at 25 C and colonies of both species, easily visible to the unaided eye, appeared within 1 week. There was …

Myxosoma Funduli Kudo 1918 (Protozoa: Myxosporida) In Fundulus Kansae: Summer Epizootiology, Stephen A. Knight, John J. Janovy Jr., William L. Current

John J. Janovy, Jr. Publications

The occurrence and distribution of the myxosporidan Myxosoma funduli on the gills of the plains killifish (Fundulus kansae) were investigated; Fundulus kansae is reported as a new host. Host samples from various sites on the South Platte River, Nebraska, were collected during the summer months of 1975 and 1976. The protozoan parasite population was shown to be overdispersed within the host population, and this distribution was similar to that described by the negative binomial equation. Demographic characteristics of the infected fish subpopulation were virtually identical to those of the whole fish population. Infection intensity was independent of gill …

Two New Zoogonid Digenea From Deep Sea Fishes In The Gulf Of Panama, Robin M. Overstreet, Mary Lou Hanson Pritchard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Panopula cavernossa gen. et sp. n. from the intestine of the brotulid Enchelybrotula (?) paucidens,/i> differs from other members of the Steganodermatinae by having testes near the anterior of the acetabulum, a posttesticular ovary, and a postequatorial acetabulum. Two related species are transferred to the genus Brachyenteron as B. pycnorganum comb. n. and B. spinosum comb. n. Neosteganoderma gillissi sp. n. from the synaphobranchid eel Synaphobranchus bathybius differs from N. glandulosum and N. polymixiae, the most similar species, because conspicuous glandular cells envelop the esophagus and pharynx, others about the genital atrium occur in elongated sacs, epithelium is lacking …

Marine Aspidogastrids (Trematoda) From Fishes In The Northern Gulf Of Mexico, Sherman S. Hendrix, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Of the aspidogastrids Multicalyx cristata, Lobatostoma ringens, Cotylogaster basiri, and C. dinosoides sp. n., the last two had not been previously known from the Gulf of Mexico. The latter differs from other members of its genus by having relatively large equatorial marginal alveoli in comparison to those at the anterior and posterior ends of the holdfast. It also possesses extensive transverse musculature connecting opposed lateral alveoli. New host records are included for all four species, and we report preadults of what appear to be the first two species.

Nematicidal Injection: Targeted Control Of Plant-Parasitic Nematodes Of Trees And Vines, D. R. Viglierchio, Armand R. Maggenti, R. V. Schmitt, G. A. Paxman

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Pressurized injection of nematicidal solutions was effective for control of nematodes within trees and vines. Significant (P = 0.01) control of Pratylenchus vulnus on grape was attained with four nonfumigants (carbofuran, oxamyl, phenamiphos, and sulfocarb) and one fumigant nematicide (DBCP). Pratylenchus penetrans was controlled (P = 0.05 and 0.1) in apples and walnuts with sulfocarb and oxamyl. This species also was controlled in apples with carbofuran and phenamiphos. The advantages of pressure injection over traditional methods of nematicide applications are discussed.

Monoecocestus Thomasi Sp. N. (Cestoda: Anoplocephalidae) From The Northern Flying Squirrel, Glaucomys Sabrinus (Shaw), In Oregon, Robert L. Rausch, C. Maser

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Monoecocestus thomasi sp. n. (Cestoda: Anoplocephalidae), from the northern flying squirrel, Glaucomys sabrinus (Shaw), in Oregon, is described and distinguished from its congeners. Monoecocestus thomasi is the sixth species of Monoecocestus to be described from nearctic rodents, and the first from a member of the family Sciuridae. Our comparisons of taxonomic characters of the nominal species of Monoecocestus in North American rodents have shown that M. giganticus Buhler 1970 is a synonym of M. americanus (Stiles 1895), both from the porcupine, Erethizon dorsatum (Linnaeus). Insemination in Monoecocestus spp. is discussed, with the conclusion that it takes place only by way …

Research For Connecticut By The Storrs Agricultural Experiment Station

Storrs Agricultural Experiment Station

No abstract provided.

Effects Of Types 1 And 2 Herpes Simplex Viruses On Several Fish Tissue Culture Systems, Raymond Charles Harry

Dissertations and Theses

The purpose of this investigation was to observe changes induced by types 1 and 2 herpes simplex viruses (HSV 1 and HSV 2) when these viruses were cultivated in fish cells. The possibility of attempting to use human strains of herpesviruses in order to transform normal fish tissue culture preparations was considered. Three different fish cell cultures were tested: CHSE-214 cell lines were derived from embryonic chinook salmon (Oncorhynchus tshawytscha), and STE-137 cell lines were obtained from embryonic steelhead trout (Salmo gairdneri). Cells isolated from immature ovaries of rainbow trout (Salmo gairdneri) were propagated …

The Immunizing Potential Of Sporulated Oocysts Of Eimeria Nieschulzi Exposed To Heat And Co-60 Gamma-Radiation, George A. Conder, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sporulated oocysts of Eimeria nieschulzi Dieben 1924, a rat coccidium, were exposed to radiation, heat, or both in an effort to attenuate the parasite. Moderate levels of each treatment or combination thereof attenuated the parasite, reduced pathogenesis (as judged by oocyst discharge during primary infection), and produced immunity to challenge when the oocysts were subsequently inoculated into rats. Thus, heat- and/or radiation-treated E. nieschulzi oocysts fed to rats could reduce pathogenesis during a primary infection and yet give good homologous protection.

Life History And Host Specificity Of Mediorhynchus Centurorum Nickol 1969 (Acanthocephala: Gigantorhynchidae), Brent B. Nickol

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Examination of 1905 arthropods from a swamp and surrounding pasture in southern Louisiana revealed larval specimens of Mediorhynchus centurorum in 8 of 228 woodroaches, Parcoblatta pensylvanica, but in no other species. The life cycle was confirmed by feeding eggs of M. centurorum to laboratory-reared woodroaches. Cystacanths later recovered from the woodroaches developed into mature worms when pipetted into esophaguses of red-bellied woodpeckers, Centurus carolinus; redheaded woodpeckers, Melanerpes erythrocephalus; yellow-shafted flickers, Colaptes auratus; and a hairy woodpecker, Dendrocopos villosus. Infection was achieved in all woodpeckers fed cystacanths at least 47 days old and the mean prepatent …

Aploparaksis Borealis Sp. N. (Cestoda: Hymenolepididae) From Passeriform And Charadriiform Birds In Chukotka And Alaska, S. K. Bondarenko, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Aploparaksis borealis sp. n. (Cestoda: Hymenolepididae), from the long-tailed jaeger, Stercorarius longicaudus Vieillot, on the Seward Peninsula, Alaska, and from the red-throated pipit, Anthus cervinus (Pallas), and the Lapland longspur, Calcarius lapponicus (Linnaeus), in Chukotka, northeastern Siberia, is described. This cestode is distinguished by the structure of the cirrus and other morphologic characters from other species of Aploparaksis Clerc 1903 in which there are 10 rostellar hooks and the vitelline gland is aporal to the ovary. A. borealis is the first species of Aploparaksis to be reported from birds representing two orders (Passeriformes and Charadriiformes).

Immunization Of Rats Against Mesocestoides Corti (Cestoda) By Subcutaneous Vaccination Of Living Tetrathyridia And By Passive Transfer With Serum, Jerry Y. Niederkorn

Journal of the Arkansas Academy of Science

Laboratory rats were subcutaneously vaccinated with 100 live tetrathyridia of Mesocestoides corti (Cestoda) and subsequently intraperitoneally challenged with 50 tetrathyridia. Necropsy 30 days postinfection revealed that vaccinated rats harbored 97.4% fewer worms compared to control rats. In a second experiment, passive transfer of immunity was accomplished by immune serum from subcutaneously vaccinated rats. Rats receiving immune serum harbored 33.4% lighter worm burdens compared to normal serum recipients.

Progress In Mastitis Control : A Simple Control Programme That Works, G R. Olney, R. K. Mitchell

Journal of the Department of Agriculture, Western Australia, Series 4

Intensive Department of Agriculture work on the mastitis problem has resulted in development of a simple control programme that works in W.A. dairy herds.

Farmern receive regular advice on their herd mastitis levels, and advice is available on overcoming specific problems.

Blood Parasites Of Squirrels From Coles And Lake Counties In Illinois, Nicholas I. Obiri

Masters Theses

Hepatozoon and microfilariae are described from the blood of fox and gray squirrels captured in Coles and Lake Counties in Illinois during 1976 and 1977. The hepatozoon species is identified as Hepatozoon griseisciuri on the basis of parasite morphology and hosts. A higher rate of infection (35.7% : 11.1%) was observed in gray squirrels than in fox squirrels and more adults were infected than juveniles. No microfilariae were found in gray squirrels but fox squirrels had a 57.1% infection rate with no difference based on age. The observed microfilariae are similar to that described by Davidson from Maryland gray squirrels …

Interrelationships Of Hypera Postica (Gyllenhal) (Coleoptera: Curculionidae) And Bathyplectes Curculionis (Thomson) (Hymenoptera: Ichneumonidae) In Illinois With Emphasis On Host-Parasite Densities, Distribution Of Parasites Within Available Hosts And Estimates Of Percent Parasitism, Robert John Barney

Masters Theses

In an attempt to better understand the host-parasite relationship of the alfalfa weevil, Hypera postica (Gyllenhal) (Coleoptera: Curculionidae) and Bathyplectes curculionis (Thomson) (Hymenoptera: Ichneumonidae), all life stages of the parasite were monitored throughout the spring. Sampling was conducted on a degree day basis from early March to late May in an alfalfa field in Mason County, Illinois.

Dissections of host alfalfa weevil larvae revealed 2nd and 3rd host instars were preferred larvae for oviposition. Peak parasite densities were found to precede peak total host larval densities, but coincided with peak densities of the preferred 2nd and 3rd instars. Larger host …

Ultrastructure Of Interlamellar Henneguya Exilis In The Channel Catfish, William L. Current, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Ultrastructural aspects of interlamellar Henneguya exilis infections in channel catfish are reported. The plasmodium wall of this form differs from that of other species in that it is composed of two outer unit membranes which give rise to a zone of numerous pinocytic canals. Single-membraned canals appeared to be a stable feature of the wall while double-membraned canals are interpreted as those actively carrying out pinocytosis. Evidence suggests that host cellular cytoplasm as well as interstitial material is taken in by plasmodia. Plasmodium wall integrity, aggregation of parasite ectoplasmic components, numbers of pinocytic canals, and number of mitochondria proximal to …

Ultrastructure Of The Sporocyst Wall During Excystation Of Isospora Endocallimici, Clarence A. Speer, Alan A. Marchiondo, Donald W. Duszynski, Sharon K. File

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sporocysts of Isospora endocallimici, a parasite of marmosets, were exposed to minimal essentials medium (MEM) or a trypsin-bile salt solution (TBS) and then fixed and prepared for trans- mission electron microscopy. Excystation occurred in TBS but not MEM. The sporocyst wall has 2 layers, a thin outer layer (15 to 110 nm thick) and a thick inner layer (65 to 180 nm thick), which is composed of 4 separate curved plates. The outer layer consists of 1 to 3 membranes interspersed with lipid droplets. In the inner layer, a thin layer of material connects the peripheral margins of 2 …

Telorchis Gutturosi Sp. N. (Trematoda: Telorchiidae) From Graptemys Pseudogeographica Gray In Nebraska, With Reports Of Additional Species Of Trematodes From Nebraska Turtles, Daniel R. Brooks, Monte A. Mayes

Harold W. Manter Laboratory of Parasitology: Faculty Publications

One species of digenean is described and 8 others reported from turtles in Nebraska. Telorchis gutturosi, described from Graptemys pseudogeographica, resembles T. stossichi, T. pseudoaculeatus, and T. pleroticus in having an acetabulum significantly smaller than the oral sucker, but differs by possessing a pharynx which is as large as the acetabulum and having the ovary only 1/10 the body length from the acetabulum. Telorchis necturi is considered the senior synonym of T. cryptobranchi; Graptemys pseudogeographica is a new host. Hapalorhynchus stunkardi is reported for the first time since its description. Based on new morphological evidence, …

Some Platyhelminth Parasites Of Sirens (Amphibia: Sirenidae) From North America, Daniel R. Brooks, Richard L. Buckner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new genus and species of digenean and a new species of cestode are described and a previously described species of digenean reported for the first time from Siren intermedia from Illinois. A new genus is proposed for a previously described digenean, and another known species of digenean reported from Siren lacertina in Florida. Progorgodera foliata from S. intermedia has a foliate hind- body characteristic of members of Phyllodistomum and multiple testes arranged in 2 longitudinal rows characteristic of members of Gorgodera. Ophiotaenia sireni from the same host resembles O. filaroides in number of testes, O. amphiumae and O. …

Two Tetraphyllidean Cestodes From The Freshwater Stingray Potamotrygon Magdalenae Dumeril 1852 (Chondrichthyes: Potamotrygonidae) From Colombia, Daniel R. Brooks, Thomas B. Thorson

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Two new tetraphyllidean cestodes are described from the freshwater stingray Potamotrygon magdalenae from Colombia. The new genus Potamotrygonocestus is proposed for P. magdalenensis, which differs from Pedibothrium by having a bilobed rather than X-shaped ovary, and simple rather than bifid hooks. It further differs from Pachybothrium hutsoni by having bothridial hooks inserted immediately posterior to an accessory sucker rather than inserted in the middle of a thick muscular pad. Potamotrygonocestus magdalenensis is unique among known tetraphyllideans by possessing vitellaria which are compact, cylindrical bands in the lateral portions of the middle of the proglottid. The second new species, Rhinebothrium …

Fabespora Vermicola Sp. N., The First Myxosporidan From A Platyhelminth, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The myxosporidan Fabespora vermicola sp. n., tentatively placed in the family Myxidiidae, is described from the digenean Crassicutis archosargi in an estuarine fish. The myxosporidan becomes the first known from a platyhelminth and the third from an invertebrate. It differs from the only other member of the genus primarily by having polar filaments 32 to 44 μm long rather than about 8 μm, and an infection with it can stop reproduction in the digenean host either by physical means or by necrosis of gametes.

A Redescription Of Crassicutis Archosargi, A Digenean Exhibiting An Unusual Tegumental Attachment, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The homalometrine Crassicutis archosargi from the northern Gulf of Mexico is redescribed, and some related digeneans are discussed. Numerous unidentified refractile bodies occurred in the tegument and other tissues of a few specimens of C. archosargi, and an intimate relationship between the parasite and its piscine host resulting from a modified tegument is reported for others. Binding by the described adhesive tegument increases the number of known ways digeneans attach to their hosts. Several specimens also harbored a histozoic myxosporidan in several tissues and a flagellate, Hexamita sp., in their ceca.

Endoparasites Of Cats In Two Central Utah Communities, Thomas W. Sawyer

Theses and Dissertations

A survey of the endoparasites of 100 stray cats in central Utah was conducted. Each cat was euthanized, and the entire alimentary tract, gall bladder, bile duct, heart, respiratory and excretory systems were excised and examined for endoparasites. Fecal samples were examined by a sugar flotation technique. Parasites identified and number of cats infected were: Isospora felis 5; I. rivolta, 4; Dipylidium caninum, 1; Hydatigera taeniaformis, 10; Toxocara mystax, 43; and Physaloptera sp., 2. One ectoparasite, Cheyletiella parasitivorax, was identified in the feces of 4 cats. Dipylidium caninum and T. mystax are known to cause accidental infection in humans and …

Anesthetization Of Morphologically Similar Species With Carbon Dioxide For Separation And Culture, Gregory R. Noel, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Nematologists frequently encounter congeneric species in sampling for nematodes. No convenient method for the separation of morphologically similar species has been described. For Meloidogyne spp., inoculum consisting of hundreds of eggs can be easily obtained from a single egg mass and a pure population reared on a suitable host. The separation and culture of other plant parasitic nematode species, such as Paratrichodorus spp., is often more difficult. The species may be separated by placing a single female in the rhizosphere of a host plant, but the chances of a single nematode increasing to a high population in a short time …

Concurrent Infection Of Trypanosoma Musculi Kendell, 1906, And Schistosoma Mansoni Sambon, 1907 In Mice, Berhane Andeberhan

Loma Linda University Electronic Theses, Dissertations & Projects

The possible effects of ablastin on Schistosoma mansoni were investigated by (1) establishing a concurrent infection of Trypanosoma musculi and Schistosoma mansoni in mice, and (2) by obtaining Trypanosoma lewisi immune rat serum (ablastin) and injecting it into S. mansoni infected mice. These procedures were followed on days 4, 14 and 28 of S. mansoni infection. For the groups that were given immune rat serum, controls were kept into which normal rat serum was injected.

Ablastin does not have any effect on the development or morphology of the S. mansoni worms as tested in this study. Although ablastin inhibits glycolytic …