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Paleopharmacology, Karl Reinhard

Karl Reinhard Publications

An emerging field devoted to the exploration of the archaeological record for evidence of medicinal plants.

In the future, archaeology will have a role in pharmaceutical research. In the late twentieth century, much of the world’s pharmaceutical research has been based on ethnographic documentation of tribally recognized medicinal plants. Once the active chemical compounds in the plants are identified, they are either extracted or synthesized for commercial use. The American film Medicine Man depicts this approach. As shown in the film, such pharmacological research is threatened by declining tribal populations with loss of traditional herbal knowledge coupled with declining biodiversity. …

The Systematics Of The Genus Trichinella With A Key To Species, K. D. Murrell, J. Ralph Lichtenfels, Dante S. Zarlenga, E. Pozio

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The authors review the major biological, biochemical, and molecular characters that are used to distinguish the seven Trichinella species (T. spiralis, T. nativa, T. britovi, T. pseudospiralis, T. murrelli, T. nelsoni, T. papuae) and three genotypes whose taxonomic status is yet uncertain (T-6, T-8, T-9). A comparison of host specificity, morphology, reproductive abilities, nurse cell development and freeze resistance is presented, along with useful biochemical and molecular markers. Finally, this information is used to construct a diagnostic key for the species. A phylogenetic classification of the species is needed.

A New Eimeria Sp. From The Plumbeous Central American Caecilian, Dermophis Mexicanus (Amphibia: Gymnophiona) From Volcán, Tajumulco, Department Of San Marcos, Guatemala, Ingrid M. Asmundsson, Jonathan A. Campbell, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fresh fecal samples from 5 caecilians (Dermophis mexicanus) were collected and examined for coccidia in the summer of 1998. The caecilians were collected in the Department of San Marcos, Guatemala. Two of the 5 (40%) specimens of caecilians contained an Eimeria species that is described here as new. This represents the first coccidia described from a gymnophionian host. Sporulated oocysts are spheroidal to subspheroidal, 19.5 × 17.7 (16–23 × 15–21) μm, micropyle and oocyst residuum are absent, and 3 (or more) polar granules are always present. Sporocysts are ovoidal, 11.0 × 7.2 (10–12 × 6–9); a Stieda body …

Coccidia (Apicomplexa: Eimeriidae) Of The Mammalian Order Insectivora, Donald W. Duszynski, Steve J. Upton

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The coccidia (protistan phylum Apicomplexa Levine, 1970) comprise a large group of obligate intracellular parasites commonly found in all classes of vertebrate hosts and in some invertebrates. This review focuses on those species within the Eimeriidae because they are among the most prevalent and speciose of all parasite groups, and there is no taxonomic summation currently available for those species that infect insectivores. All published species descriptions in the genera Cyclospora, Eimeria and Isospora that infect insectivores are reviewed and evaluated. Some of the named species are invalid, either because rules concerning the naming of new species (International …

Life Cycle Of Calyptospora Funduli (Apicomplexa: Calyptosporidae), John W. Fournie, Wolfgang K. Vogelbein, Robin M. Overstreet, William E. Hawkins

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The taxonomic status of the extraintestinal piscine coccidium Calyptospora funduli is based in part on its requirement of an intermediate host (the daggerblade grass shrimp Palaemonetes pugio). In the present study, grass shrimp fed livers of Gulf killifish (Fundulus grandis) infected with sporulated oocysts of C. funduli exhibited numerous sporozoites suspended in the intestinal contents when fresh squash preparations were examined by light microscopy. Using this method, sporozoites were not seen in intestinal epithelial cells of the grass shrimp or in any other cell types. Ultrastructural examination, however, revealed sporozoites in the cytoplasm of the gut basal …

Six New Host Records And An Updated List Of Wild Hosts For Neobenedenia Melleni (Maccallum) (Monogenea: Capsalidae), Stephen A. Bullard, George W. Benz, Robin M. Overstreet, Ernest H. Williams Jr., Jay Hemdal

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Six new host records and an updated list of wild hosts for Neobenedenia melleni (MacCallum) (Monogenea: Capsalidae) are provided. We report specimens of N. melleni from the skin of a whitefin sharksucker (Echeneis neucratoides Zuieuw [Echeneidae]) caught off Mayagüez, Puerto Rico; from the skin of a mosquitofish (Gambusia xanthosoma Greenfield [Poeciliidae)) caught in Little Salt Creek, Grand Cayman Island, British West Indies; from a freshwater immersion bath of red grouper (Epinephelus morio (Valenciennes) [Serranidae] caught in the Gulf of Mexico off Sarasota, Florida, U.S.A.; from the skin of a garden eel (Heteroconger hassi (Klausewitz and Eibl-Eibesfeldt) …

Reproductive Biology Of Cobia, Rachycentron Canadum, From Coastal Waters Of The Southern United States, Nancy J. Brown-Peterson, Robin M. Overstreet, Jeffrey M. Lotz, James S. Franks, Karen M. Burns

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Reproductive biology of the cobia, Rachycentron canadum, is described from four coastal areas in the southern United States. Samples were obtained from recreational fishermen between December 1995 and November 1997 from the southeastern United States (Morehead City, NC, to Cape Canaveral, FL), the eastern Gulf of Mexico (Ft. Myers to Crystal River, FL), the north-central Gulf of Mexico (Destin, FL, to Chandeleur Islands, LA) and the western Gulf of Mexico (Port Aransas, TX). Histological evidence of spawning occurred from April through September in all areas. Some female cobia (17-32%) throughout the Gulf of Mexico had spent or regressed ovaries …

Syncoelium Vermilionensis Sp. N., (Hemiuroidea: Syncoeliidae) And New Records For Members Of Azygiidae, Ptychogonimidae, And Syncoeliidae Parasitizing Elasmobranchs In The Gulf Of California, Stephen S. Curran, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

During July-September 1993 and May 1996, a survey of the metazoan parasites of 817 elasmobranchs from the Gulf of California, representing 42 species, was conducted at 14 sites along the western portion of the Gulf of California. Elasmobranchs were obtained from local commercial fishers. In this paper, we report all digeneans collected during the survey, excluding the gorgoderids, which will be presented in a subsequent paper. Four species from three hemiuroid families were collected, none of which was known previously from the Gulf of California. Syncoelium vermilionensis sp. n. (Syncoeliidae), a species that differs most notably from its congeners by …

Flea News 59 (December 1999), Robert E. Lewis

Flea News

Obituary: Li Kuei Chen (January 6, 1911-October 21, 1999)

Miscellanea

Book reviews: Eustorgio Méndez, Insectos y otros artrópodos de importancia médica y veterinaria; Mammals of the Eastern United States, 3rd edition. John O. Whitaker, Jr. and W. J. Hamilton, Jr., 1998; Walker's Mammals of the World: Volume I, 6th edition. Ronald M. Nowak, 1999

Siphonaptera literature

Fatal Cysticercosis By Taenia Crassiceps (Cyclophyllidea: Taeniidae) In A Presumed Immunocompromised Canine Host, Eric P. Hoberg, William Ebinger, James A. Render

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cysticercosis in a canine host (Canis jamiliaris) attributable to the taeniid cestode Taenia crassiceps is reported for the first time in North America. Numerous parent and daughter cysticerci occurred in a massive intrapleural and intraperitoneal infection in an apparently immunocompromised host. The largest cysticerci were ovoid to elongate. 5-9 mm in maximum length. and armed with 32-34 rostellar hooks in two rows; small hooks measured 114-143 μm long (avg. = 124 ± 8.2 μm). and large hooks were 156-180 μm (avg. = 163 ± 7.4 μm). Taenia crassiceps is widespread in boreal North America and, like a number …

American Society Of Parasitologists Newsletter, V. 21, No. 3, August 25, 1999, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.

Cross-Transmission Studies With Eimeria Arizonensis, E. Arizonensis-Like Oocysts And Eimeria Langebarteli: Host Specificity At The Genus And Species Level Within The Muridae, John A. Hnida, Donald Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cross-transmission experiments were done using sporulated oocysts of Eimeria arizonensis from Peromyscus truei and Peromyscus maniculatus, and oocysts of 2 putative species that resemble E. arizonensis, i.e., Eimeria albigulae from Neotoma albigula, and Eimeria onychomysis from Onychomys leucogaster. Oocysts of each species were inoculated into representatives of P. maniculatus and the latter 2 rodent species. Other experiments were conducted wherein oocysts of Eimeria langebarteli from Peromyscus leucopus were given to P. truei and P. maniculatus. Oocysts of E. arizonensis from P. truei and P. maniculatus could be transmitted only to P. maniculatus; likewise, …

Differentiation Of Mexican Species Of Haematoloechus Looss, 1899 (Digenea: Plagiorchiformes): Molecular And Morphological Evidence, Virginia León-Règagnon, Daniel R. Brooks, Gerardo Perez-Ponce De Leon

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Molecular evidence is interpreted in the light of morphology to examine the validity of several species of Haematoloechus described as Mexican endemics. Internal transcribed spacers 1 and 2 and 28S ribosomal genes were sequenced for 11 isolates. Phylogenetic analysis of separate partitions and combined databases was conducted. Results were analyzed, in the light of morphological evidence. Haematoloechus macrorchis is proposed as a junior synonym of Haematoloechus longiplexus. Haematoloechus pulcher is a sibling species with Haematoloechus complexus in Lerma wetlands. In Mexico, Haematoloechus medioplexus is distributed along the east coast coinciding with the distribution of Rana berlandieri. The sister …

Emerging Marine Diseases: Climate Links And Anthropogenic Factors, C. D. Harvell, K. Kim, J. M. Burkholder, R. R. Colwell, P. R. Epstein, D. J. Grimes, E. E. Hoffmann, E. K. Lipp, A. D.M.E. Osterhaus, Robin M. Overstreet, J. W. Porter, G. W. Smith, G. R. Vasta

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Mass mortalities due to disease outbreaks have recently affected major taxa in the oceans. For closely monitored groups like corals and marine mammals, reports of the frequency of epidemics and the number of new diseases have increased recently. A dramatic global increase in the severity of coral bleaching in 1997-98 is coincident with high El Niño temperatures. Such climate-mediated, physiological stresses may compromise host resistance and increase frequency of opportunistic diseases. Where documented, new diseases typically have emerged through host or range shifts of known pathogens. Both climate and human activities may have also accelerated global transport of species, bringing …

Effects Of Plasma Hiv Rna, Cd4+ T Lymphocytes, And The Chemokine Receptors Ccr5 And Ccr2b On Hiv Disease Progression In Hemophiliacs, Eric S. Daar, Sharyne Donfield, Edward Gomperts, Margaret Hilgartner, Keith Hoots, David Chernoff, Cheryl Winkler, Stephen J. O'Brien, Hemophilia Growth And Development Study

Biology Faculty Articles

We have investigated the effects of plasma HIV RNA, CD4⁺ T lymphocytes and chemokine receptors CCR5 and CCR2b on HIV disease progression in hemophiliacs. We prospectively observed during follow-up 207 HIV-infected hemophiliacs in the Hemophilia Growth and Development Study. Plasma HIV RNA was measured on cryopreserved plasma from enrollment using the Chiron Corporation bDNA (version 2.0) assay. Genotype variants CCR2b-641 and CCR5-Δ32 were detected using standard molecular techniques. Those with the mutant allele for CCR2b, and to a lesser extent CCR5, had lower plasma HIV RNA, and higher CD4⁺ T lymphocytes than did those without these …

Systematics Of The Eucestoda: Advances Toward A New Phylogenetic Paradigm And Observations On The Early Diversification Of Tapeworms And Vertebrates, Eric P. Hoberg, Scott Lyell Gardner, Ronald A. Campbell

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Evolutionary relationships of the Eucestoda have received intense but sporadic attention over the past century. Since 1996, the landscape has dramatically changed with respect to our knowledge of the phylogenetic relationships among the tapeworms. The 2nd International Workshop for Tapeworm Systematics (IWTS) held in Lincoln, Nebraska in October of that year provided the catalyst for development of novel hypotheses for inter-and intra-ordinal phylogeny. The working-group structure of the 2nd IWTS and results of phylogenetic studies are briefly introduced in the present manuscript. Higher-level phylogenies derived from parsimony analysis of independent data bases representing comparative morphology or molecular sequences were largely …

Role Of Mitogen-Activated Kinases In Cd40-Mediated T Cell Activation Of Monocyte/Macrophage And Vascular Smooth Muscle Cell Cytokine/Chemokine Production, Denise M. Milhorn

Electronic Theses and Dissertations

This dissertation represents efforts to determine the functional consequences acquired by vascular smooth muscle cells (SMC) in response to CD40 ligation by activated CD154+ T cells, and to elucidate components of the signaling pathway(s) activated in response to CD40 signaling in both monocytes and SMC. To study the consequences of CD40 stimulation, primary human monocytes and aortic SMC were treated with plasma membranes purified from CD154 + , CD4+ T cells. The results presented in this dissertation demonstrate that SMC, like monocytes/macrophages, are capable of interacting with T cells in a manner that results in reciprocal activation events. SMC were …

American Society Of Parasitologists Newsletter, V. 21, No. 2, [Summer 1999], Scott Lyell Gardner

American Society of Parasitologists: Newsletter

An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.

Optimal Replication Activity Of Vesicular Stomatitis Virus Rna Polymerase Requires Phosphorylation Of A Residue(S) At Carboxy-Terminal Domain Ii Of Its Accessory Subunit, Phosphoprotein P, Leroy N. Hwang, Nathan Englund, Tapas Das, Amiya K. Florida, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein, P, of vesicular stomatitis virus (VSV) is a key subunit of the viral RNA-dependent RNA polymerase complex. The protein is phosphorylated at multiple sites in two different domains. We recently showed that specific serine and threonine residues within the amino-terminal acidic domain I of P protein must be phosphorylated for in vivo transcription activity, but not for replication activity, of the polymerase complex. To examine the role of phosphorylation of the carboxy-terminal domain II residues of the P protein in transcription and replication, we have used a panel of mutant P proteins in which the phosphate acceptor sites …

Flea News 58 (June 1999), Robert E. Lewis

Flea News

Obituary: Lindolpho Rocha Guimarães (January 24, 1908-November 7, 1998)

Miscellanea

Siphonaptera literature

Maternal Modulation Of Neonatal Immunity, Omar R. Fagoaga

Loma Linda University Electronic Theses, Dissertations & Projects

Although the neonatal immune system is functionally immature, immune response capabilities are not the same in all newborns. The objective of this thesis was to determine development of immunocompetence and test the hypothesis that activation of maternal inflammatory immunity during pregnancy influences maturation of the neonatal immune system.

Lymphocytes in blood and spleen were immunophenotyped by flow cytometric analysis to determine differentiation characteristics, and splenocytes were assessed for cytokine production capabilities. In normal Th2-prone outbred (CD-1) mice, an adult repertoire of naïve lymphocytes has developed by day 10 and memory cells by day 20, in blood and spleen. However, in …

Review Of The Avian Brood Parasites: Deception At The Nest By Paul A. Johnsgard; Oxford University Press, 1997, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Review of The Avian Brood Parasites: Deception at the Nest by Paul A. Johnsgard, published by Oxford University Press, 1997, ISBN: 0-19-511042-0.

Scyphophyllidium Uruguayense N. Sp. (Eucestoda: Tetraphyllidea) In Mustelus Mento (Cope, 1877) (Chondrichthyes: Carcharhiniformes: Triakidae) From La Paloma, Uruguay, Daniel R. Brooks, Fernando Marques, Carla Perroni, Corina Sidagis

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species of Scyphophyllidium inhabits Mustelus mento near La Paloma, Uruguay. It resembles Scyphophyllidium giganteum from the Atlantic Ocean and specimens identified as S. giganteum from California by having anapolytic strobilae 155-258 mm long, 250-300 craspedote proglottids, scoleces 1.2-1.4 mm wide, necks 34-41 mm long, immature and mature proglottids wider than long, gravid proglottids wider than long to longer than wide, genital pores averaging 28% of proglottid length from the anterior end, relatively flat ovaries with digitiform lobes reaching the lateralmost extent of the testicular field, vitellaria in 2 fields converging toward the proglottid midline, straight and short cirrus …

Pararhinebothroides Hobergi N. Gen. N. Sp. (Eucestoda: Tetraphyllidea) In Urobatis Tumbesensis (Chondrichthyes: Myliobatiformes) From Coastal Ecuador, David Zamparo, Daniel R. Brooks, Ramiro Barriga

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species of tetraphyllidean eucestode inhabiting Urobatis tumbesensis from inshore waters of southeastern Ecuador shares 3 synapomorphies with Rhinebothroides spp.: apical bothridial suckers poorly differentiated from the marginal loculi, internal seminal vesicles, and insertion of the vas deferens dorsally closer to the poral than the aporal end of the cirrus sac. The new species differs from Rhinebothroides spp. by lacking medial bothridial septa and loculi and having symmetrical ovarian arms, and possesses an apparent autapomorphic trait by having the vas deferens tapering to a narrow tube before entering the cirrus sac, extending posteriorly to the posterior end of the …

Macroevolutionary Patterns Of Male Reproductive Investment In A Clade Of Parasitic Hermaphrodites, David Zamparo, Deborah A. Mclennan, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The Eucestoda is particularly relevant for questions concerning reproductive investment in male gametes because no other parasitic group displays such diversity in testis size and number within and among species. This diversity has long been used as a valuable taxonomic character, but few researchers have ever investigated its evolutionary significance. In this paper we investigate the evolution of testis number and size within Rhinebothroides (Platyhelminthes: Eucestoda). Our comparative, phylogenetic analysis revealed that overall allocation to male functions, as measured by relative testicular area, does not change within the clade, even though the packaging of that investment in numerous testes is …

Species: Turning A Conundrum Into A Research Program, Daniel R. Brooks, Deborah A. Mclennan, E. C. Bernard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The most appropriate ontological basis for understanding the role of species in evolutionary biology is the Evolutionary Species Concept. The ESC is not an operational concept, but one version of the Phylogenetic Species Concept is. Linking the ontology of species with the epistemological basis of actual biological studies requires that we specify both a discovery mode for identifying collections of organisms that we believe are evolutionary species, and a series of evaluation criteria for assessing those entities we have discovered. Simply naming a collection of specimens, no matter how strong one’s evolutionary beliefs, is not sufficient for declaring that evolutionary …

Histogenesis In The Metacestode Of Echinococcus Vogeli And Mechanism Of Pathogenesis In Polycystic Hydatid Disease, Robert L. Rausch, Antonio D'Alessandro

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Histogenesis of the metacestode of Echinococcus vogeli was traced mainly in rodents inoculated intraperitoneally with finely minced infective vesicles. The fragments aggregated in the peritoneal cavity and coalesced, forming structures (plaques) from which primary vesicles arose. From primordia in their germinal tissue, exogenous vesicles developed, enlarged, and migrated outward to the surface of the laminated membrane, where they remained attached and proliferated. Each unit of vesicles so formed retained discrete identity and, within 6-8 months, acquired an adventitia; thereafter, exogenous multiplication ceased and endogenous proliferation supervened. Large numbers of daughter cysts arose in the germinal tissue lining chambers within the …

Tb Or Not Tb: Disease And The Adaptiveness Of Political Borders, Ibpp Editor

International Bulletin of Political Psychology

This article describes adaptive and nonadaptive aspects of political borders at the threshold of the 21st century.

Recommendations From A Satellite Meeting (International Symposium To Commemorate The 90th Anniversary Of The Discovery Of Chagas Disease, April 11-16 1999, Rio De Janeiro, Brazil), Alejandro O. Luquetti, Aluízio Rosa Prata, Alvaro Moncayo, Alvaro Romanha, Ana Maria Jansen, Bianca Zingales, Carlos Morel, Carlos Ponce, Egler Chiari, Elisa Cupolillo, Elisa Ponce, Felipe Guhl, Hooman Momen, José Rodrigues Coura, Mario Steindel, Michael Miles, Michel Tibayrenc, Octavio Fernandes, Riva Oliveira, Rodrigo Zeledón, Sonia Gumes Andrade, Toby Vincent Barrett, Vanize Macêdo, Zigman Brener

Harold W. Manter Laboratory of Parasitology: Library Materials

During this symposium the standardization of the nomenclature of Trypanosoma cruzi strains was discussed, in a parallel session, with a view to facilitating the use and understanding of a common nomenclature that would serve not only taxonomists but the general community of researchers working with T. cruzi.

The diversity in the behavior and morphology of T. cruzi isolates was soon recognized after the discovery of Chagas disease. Since then a variety of biochemical and molecular techniques have revealed the great genetic diversity present in strains of this parasite. Different investigators have described this diversity by using various …

Margolisianum Bulbosum N. Gen., N. Sp. (Nematoda: Philometridae) From The Southern Flounder, Paralichthys Lethostigma (Pisces: Bothidae), In Mississippi Sound, Usa, Reginald B. Blaylock, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species of a philometrid nematode, Margolisianum bulbosum, is described from the subcutaneous tissue in the mouth (larvigerous females), head (males, ovigerous, and larvigerous females), and eye (preovigerous females) of the southern flounder, Paralichthys lethostigma, from Mississippi Sound. It is placed in a new genus diagnosed by the combination of 8 large, paired but separate cephalic papillae; no inner cephalic papillae; an esophagus with a separate, muscular anterior bulb; a prominent mononuclear esophageal gland; and variable, irregularly distributed cuticular bosses in the females, as well as a vestigial rectum, particularly in larvigerous females. Some female specimens exhibit …