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Immunogenetic Association Underlying Severe Covid-19, Kendall Mccoy, Autumn Peterson, Yun Tian, Yongming Sang

Agricultural and Environmental Sciences Faculty Research

SARS-CoV2 has caused the current pandemic of new coronavirus disease 2019 (COVID-19) worldwide. Clinical outcomes of COVID-19 illness range broadly from asymptotic and mild to a life-threatening situation. This casts uncertainties for defining host determinants underlying the disease severity. Recent genetic analyses based on extensive clinical sample cohorts using genome-wide association studies (GWAS) and high throughput sequencing curation revealed genetic errors and gene loci associated with about 20% of life-threatening COVID-19 cases. Significantly, most of these critical genetic loci are enriched in two immune signaling pathways, i.e., interferon-mediated antiviral signaling and chemokine-mediated/inflammatory signaling. In line with these genetic profiling studies, …

Epidemiology And Pathophysiology Of Common Skin Diseases In West Africa: An Immunodermatological Framework, Osazomon Imarenezor

All HCAS Student Capstones, Theses, and Dissertations

This capstone reviews the common skin diseases on a global scale. With these dermatoses being further funneled into Africa and then magnified into common West African dermatoses, the meta-analyses of literature available paints a clear picture of the epidemiological & pathological factors and their contribution to the skin disease. Each article analysed in this analysis was taken from a 20-year span of January 2000 to December 2019. The selection of articles was fine-tuned by identifying the distribution of skin disease, revealing the populations affected (age, gender, ethnicity, etc), the main causes, country of origin, the prognosis of disease, and the …

Bifurcation Analysis Of Newcastle Disease Eco-Epidemiological Model In The Presence Of Vaccination: A Case Of The Backyard Chicken, Furaha M. Chuma, Gasper G. Mwanga

Journal of Humanities and Social Sciences

Journal of Education, Humanities and Sciences, Volume 9 No. 2, 2020: 42–59 © Dar es Salaam University College of Education (DUCE), 2020 Bifurcation Analysis of Newcastle Disease Eco-epidemiological Model in the Presence of Vaccination: A Case of the Backyard Chicken Furaha M. Chuma Dar es Salaam University College of Education University of Dar es Salaam [email protected] Gasper G. Mwanga Dar es Salaam University College of Education University of Dar es Salaam [email protected] Abstract Newcastle is an infectious disease caused by the avian paramyxomovirus-1. It occurs most frequently as a disease of Avians (e.g., chicken, ducks, pigeons, guinea fowls and other …

Strategies To Improve Coverage Of Typhoid Conjugate Vaccine (Tcv) Immunization Campaign In Karachi, Pakistan, Farah Qamar Naz, Rabab Batool, Sonia Qureshi, Miqdad Ali, Tahira Sadaf, Junaid Mehmood, Khalid Iqbal, Akram Sultan, Noah Duff, Mohammad Tahir Yousafzai

Department of Paediatrics and Child Health

The emergence and spread of extensively drug-resistant (XDR) typhoid in Karachi, Pakistan led to an outbreak response in Lyari Town, Karachi utilizing a mass immunization campaign with typhoid conjugate vaccine (TCV), Typbar TCV®. The mass immunization campaign, targeted Lyari Town, Karachi, one of the worst affected towns during the XDR typhoid outbreak. Here we describe the strategies used to improve acceptance and coverage of Typbar TCV in Lyari Town, Karachi. The mass immunization campaign with Typbar TCV was started as a school- and hospital-based vaccination campaign targeting children between the age of 6 months to 15 years old. A dose …

Carbapenemase Screening In An Irish Tertiary Referral Hospital: Best Practice, Or Can We Do Better?, Sinéad Fahy, J. A. O'Connor, D. O'Brien, L. Fitzpatrick, M. O'Connor, J. Crowley, M. Bernard, Roy D. Sleator, Brigid Lucey

Department of Biological Sciences Publications

Initially, United States Centre of Disease Control (CDC) recommended culture-based CPE screens; using broth enrichment in tryptone soy broth (TSB), followed by culture onto MacConkey agar with a carbapenem disc. However, this protocol is both time consuming (approx. 48hrs) and labour intensive, making it impractical for routine screening, particularly in a busy hospital laboratory [10]. Some improvements have been observed using commercially available culture plates, particularly in terms of decreased turnaround times (approx. 24hrs) and increased sensitivity/specificity for CPE producers; mSuperCarba (CHROMagar) media, for example has shown an increased sensitivity rate of 83% compared to 69% for MacConkey culture with …

November 16, 2020: Letter From The President- Covid-19 Update, Kettering University

Communication Updates

No abstract provided.

Interleukin-17a Facilitates Chikungunya Virus Infection By Inhibiting Ifn-Α2 Expression, Biswas Neupane, Dhiraj Acharya, Farzana Nazneen, Gabriel Gonzalez-Fernandez, Alex Sutton Flynt, Fengwei Bai

Faculty Publications

Interferons (IFNs) are the key components of innate immunity and are crucial for host defense against viral infections. Here, we report a novel role of interleukin-17A (IL-17A) in inhibiting IFN-α2 expression thus promoting chikungunya virus (CHIKV) infection. CHIKV infected IL-17A deficient (Il17a^−/−) mice expressed a higher level of IFN-α2 and developed diminished viremia and milder footpad swelling in comparison to wild-type (WT) control mice, which was also recapitulated in IL-17A receptor-deficient (Il17ra^−/−) mice. Interestingly, IL-17A selectively blocked IFN-α2 production during CHIKV, but not West Nile virus (WNV) or Zika virus (ZIKV), infections. Recombinant IL-17A …

November 15, 2020: Covid-19 Campus Update, Kettering University

Communication Updates

We are currently evaluating the full extent and impact on our campus community of the announcement by the Michigan Governor on Sunday evening. This announcement includes limitations to be imposed statewide effective this Wednesday.

Actions to be taken by the University to be in compliance with local and state directives will be determined and communicated promptly to the campus community.

Applying Immuno-Epidemiology Principles To Violence, Anna H. Sisk, Nina H. Fefferman, Judy Day, Patricia Bamwine

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

The Effect Of Fcgammariia Polymorphisms On Dengue Outbreak Severity, Leah Darwin, Richard V. Clarke

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Modeling And Preparedness: The Transmission Dynamics Of Covid-19 Outbreak In Provinces Of Ecuador, Carlos E. Bustamante Orellana, Jordy Cevallos-Chavez, Cesar Montalvo, Jeff Sulliavan, Edwin Michael, Anuj Mubayi

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

A Mathematical Model Of The Effect Of Social Distancing On The Spread Of Covid-19, Anna Singley, Hannah Callender Highlander

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

In Silico Modelling For The Treatment Of Gastric Cancer, Leonardo F. Martinez, Diana Gamboa, Paul A. Valle

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Mobility Impact In The Spreading Of Covid-19 In Ecuador, Jordy Cevallos-Chavez, Carlos E. Bustamante Orellana, Cesar Montalvo, Anuj Mubayi, Edwin Michael, Edwin Michael

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Impact Of Porcine Arterivirus, Influenza B, And Their Coinfection On Antiviral Response In The Porcine Lung, Damarius S. Fleming, Laura C. Miller, Yun Tian, Yonghai Li, Wenjun Ma, Yongming Sang

Agricultural and Environmental Sciences Faculty Research

Interferon (IFN) cytokines induce an autonomous antiviral state in cells of the infected site to restrict virus spreading and critically regulate overall antiviral response. The antiviral state leads to host protection through expression of hundreds of IFN-stimulated genes that restrict viral infection through multiple mechanisms, for example, directly in viral genome degradation and indirectly through cellular metabolic inhibition. Young pigs were split into four treatment groups: control, porcine reproductive and respiratory syndrome virus (PRRSV, also known as porcine arterivirus) infected, influenza B virus (IBV) infected, and IBV/PRRSV coinfection. Lung tissue was collected at 3, 5, and 7 days post infection …

November 11, 2020: Letter From The President- Covid-19 Town Hall Meeeting, Kettering University

Communication Updates

All students, faculty and staff are invited to join members of the Kettering University’s COVID-19 Response Team for a virtual Town Hall to discuss COVID-19 and the safety measures put in place on campus.

Mucosal Immune System Of African Lungfish, Ryan D. Heimroth

Biology ETDs

Conquering land was an apex moment in the evolution of vertebrates. Physiologically, the first vertebrates to transition to life on land had to adapt in order to survive in an oxygen-rich environment where gravitational forces and limited water prevailed. Several extant vertebrates including anurans and lungfish, survive droughts by undergoing aestivation. Unfavorable environmental conditions trigger lungfish to aestivate. Aestivation is a process of metabolic torpor wherein the fish will encase itself in a mucus cocoon and not move until favorable environmental conditions (food and water) are reintroduced. Aestivation can be reproduced in laboratory settings and, thus, lungfish can be used …

November 3, 2020: Covid-19 Campus Update, Kettering University

Communication Updates

No abstract provided.

November 2, 2020: Covid-19 Campus Update, Kettering University

Communication Updates

No abstract provided.

Neutrophil-To-Lymphocyte Ratio: A Biomarker To Monitor The Immune Status Of Astronauts, Amber M. Paul, Siddhita D. Mhatre, Egle Cekanaviciute, Ann-Sofie Schreurs, Candice G.T. Tahimic, Ruth K. Globus, Sulekha Anand, Brian E. Crucian, Sharmila Bhattacharya

Publications

A comprehensive understanding of spaceflight factors involved in immune dysfunction and the evaluation of biomarkers to assess in-flight astronaut health are essential goals for NASA. An elevated neutrophil-to-lymphocyte ratio (NLR) is a potential biomarker candidate, as leukocyte differentials are altered during spaceflight. In the reduced gravity environment of space, rodents and astronauts displayed elevated NLR and granulocyte-to-lymphocyte ratios (GLR), respectively. To simulate microgravity using two well-established ground-based models, we cultured human whole blood-leukocytes in high-aspect rotating wall vessels (HARV-RWV) and used hindlimb unloaded (HU) mice. Both HARV-RWV simulation of leukocytes and HU-exposed mice showed elevated NLR profiles comparable to spaceflight …

Investigating Microbial And Host Factors That Modulate Severity Of Clostridioides Difficile Associated Disease, Armando Lerma

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Clostridioides difficile is recognized as one of the most important pathogens in hospital and community healthcare settings. The clinical outcome of infection of toxigenic C. difficile infection (CDI) ranges from asymptomatic colonization to fulminant pseudomembranous colitis and death. In recent studies, it has been suggested that a high proportion of nosocomial CDI cases are transmitted from asymptomatic carriers which might be acting as infection reservoirs. Understanding what causes the different responses to infection could lead to the development of novel prevention and treatment strategies. Although several explanations have been proposed to explain variations in susceptibility, understanding of the exact mechanisms …

A Statistical Learning Regression Model Utilized To Determine Predictive Factors Of Social Distancing During Covid-19 Pandemic, Timothy A. Smith, Albert J. Boquet, Matthew V. Chin

Publications

In an application of the mathematical theory of statistics, predictive regression modelling can be used to determine if there is a trend to predict the response variable of social distancing in terms of multiple predictor input “predictor” variables. In this study the social distancing is measured as the percentage reduction in average mobility by GPS records, and the mathematical results obtained are interpreted to determine what factors drive that response. This study was done on county level data from the state of Florida during the COVID-19 pandemic, and it is found that the most deterministic predictors are county population density …

Varroa Destructor Mites Vector And Transmit Pathogenic Honey Bee Viruses Acquired From An Artificial Diet, Francisco Posada-Florez, Eugene V. Ryabov, Matthew C. Heerman, Yanping Chen, Jay D. Evans, Daniel E. Sonenshine, Steven C. Cook

Biological Sciences Faculty Publications

The ectoparasitic mite Varroa destructoris one of the most destructive pests of the honey bee (Apis mellifera) and the primary biotic cause of colony collapse in many regions of the world. These mites inflict physical injury on their honey bee hosts from feeding on host hemolymph and fat body cells/cellular components, and serve as the vector for deadly honey bee viruses, including Deformed wing virus (DWV) and the related Varroa destructor virus-1 (VDV-1) (i.e., DWV-like viruses). Studies focused on elucidating the dynamics of Varroa-mediated vectoring and transmission of DWV-like viruses may be confounded by viruses present in …

One Out Of Ten: Low Sampling Efficiency Of Cloth Dragging Challenges Abundance Estimates Of Questing Ticks, Siiri Nyrhilä, Jani J. Sormunen, Satu Mäkelä, Ella Sippola, Eero J. Vesterinen, Tero Klemola

Harold W. Manter Laboratory of Parasitology: Library Materials

Hard ticks (Acari: Ixodidae) act as important vectors of zoonotic pathogens. For instance, Borrelia burgdorferi s.l. spirochetes pose a severe health risk as aetiological agents of Lyme borreliosis. Commonly, to study the abundance of questing (host-seeking) ticks, a 1 m2 piece of cloth is dragged over vegetation for a determined distance. Here, we designed a tick-sampling study to estimate the sampling efficiency of this standard method. We established 10 m dragging transects in a hemiboreal mixed forest patch in SW Finland for a 5-day monitoring period. Five of the transects were cloth-dragged 3× a day, whereas another five transects were …

October 29, 2020: Covid-19 Campus Update, Kettering University

Communication Updates

The COVID-19 Response Team continues to work with the Genesee County Public Health Department and with the Of ice of the President to adapt to the situation as it evolves and provide our community with the most current information regarding the COVID-19 pandemic. The University is fully committed to the health and safety of its entire community, and will continue to closely monitor this situation and any others that impact our campus. In addition to providing updates on a regular basis, the team meets regularly to review, evaluate and address immediate and future issues related to the pandemic. Members of …

October 29, 2020: Letter From The President- Covid-19 Update, Kettering University

Communication Updates

No abstract provided.

October 27, 2020: Covid-19 Campus Update, Kettering University

Communication Updates

The COVID-19 Response Team continues to work with the Genesee County Public Health Department and with the Of ice of the President to adapt to the situation as it evolves and provide our community with the most current information regarding the COVID-19 pandemic. The University is fully committed to the health and safety of its entire community, and will continue to closely monitor this situation and any others that impact our campus. In addition to providing updates on a regular basis, the team meets regularly to review, evaluate and address immediate and future issues related to the pandemic. Members of …

October 25, 2020: Covid-19 Campus Update, Kettering University

Communication Updates

The COVID-19 Response Team continues to work with the Genesee County Public Health Department and with the Of ice of the President to adapt to the situation as it evolves and provide our community with the most current information regarding the COVID-19 pandemic. The University is fully committed to the health and safety of its entire community, and will continue to closely monitor this situation and any others that impact our campus. In addition to providing updates on a regular basis, the team meets regularly to review, evaluate and address immediate and future issues related to the pandemic. Members of …

Chain-Selective Isotopic Labeling Of The Heterodimeric Type Iii Secretion Chaperone, Scc4:Scc1, Reveals The Total Structural Rearrangement Of The Chlamydia Trachomatis Bi-Functional Protein, Scc4, Thilini O. Ukwaththage, Samantha M. Keane, Li Shen, Megan A. Macnaughtan

School of Medicine Faculty Publications

Scc4 is an unusual bi-functional protein from Chlamydia trachomatis (CT) that functions as a type III secretion system (T3SS) chaperone and an RNA polymerase (RNAP)-binding protein. Both functions require interactions with protein partners during specific stages of the CT developmental cycle. As a T3SS chaperone, Scc4 binds Scc1 during the late stage of development to form a heterodimer complex, which chaperones the essential virulence effector, CopN. During the early-middle stage of development, Scc4 regulates T3SS gene expression by binding the σ66-containing RNAP holoenzyme. In order to study the structure and association mechanism of the Scc4:Scc1 T3SS chaperone complex using nuclear …

Underground Diversity: Uropodina Mites (Acari: Mesostigmata) From European Badger (Meles Meles) Nests, Przemysław Kurek, Krzysztof Nowakowski, Tomasz Rutkowski, Agnieszka Ważna, Jan Cichocki, Michał Zacharyasiewicz, Jerzy Błoszyk

Harold W. Manter Laboratory of Parasitology: Library Materials

Badgers can gather huge quantities of organic material to build their nests for winter time and to rear their cubs. Moreover, badger burrows (setts) are characterized by specific microclimate with quite stable temperature and humidity. Their fauna is poorly studied, especially in respect of saprobiontic Uropodina mites. In 2018–2019, we monitored 94 badger setts to search for nest material that had been thrown away during cleaning of the chambers after mating and winter sleep. In the collected material from 32 badger nests, we found 413 Uropodina mites of 16 species, in various stages of development (adults, protonymphs, and deutonymphs). The …