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Improved Vitamin B12 Test May, John W. Newman, Lindsay H. Allen, Marcia Wood Apr 2013

Improved Vitamin B12 Test May, John W. Newman, Lindsay H. Allen, Marcia Wood

Agricultural Research Magazine

Vitamin B12 helps your body perform many vital chores, including forming healthy red blood cells; keeping your brain functioning smoothly; and processing (metabolizing) the fats, proteins, and carbohydrates in foods that you eat.

Like all vitamins, B12 is a micronutrient, meaning that we need it in only very small amounts. We get B12 from animal products— meats, fish, poultry, eggs, cheese, and yogurt, for instance—or from B12-fortified foods, notably breakfast cereals. We can also obtain it from nutritional supplements, such as B12 tablets or multivitamin pills. People who need to boost their B12 levels quickly may do so via shots …


Editorial: The Nutrition Transition- The Same, But Different, Aydin Nazmi, C. Monteiro Apr 2013

Editorial: The Nutrition Transition- The Same, But Different, Aydin Nazmi, C. Monteiro

Food Science and Nutrition

No abstract provided.


Cultivating Change: Women Transforming Holland’S Food System, Meg Vandeusen Apr 2013

Cultivating Change: Women Transforming Holland’S Food System, Meg Vandeusen

Independent Study Project (ISP) Collection

Women are influential in the production and reproduction of food systems. Historically, domesticity positioned women as gatekeepers to their family’s food consumption; these food practices have in turn informed cultural codes that construct personal identity. Feminist scholars have investigated how cooking and feeding practices affect a woman’s perception of self and interaction with society. Both practices are viewed as a simultaneous burden and pleasure, as an act of victimization and empowerment. These binaries must be deconstructed in order to create change within the food system. The impact of women taking control of the full cycle – gaining knowledge of where …


Development Of Value Added Products From Spent Kluyveromyces Marxianus Yeast, Rahul Sharma Apr 2013

Development Of Value Added Products From Spent Kluyveromyces Marxianus Yeast, Rahul Sharma

Theses

Biochemical analysis of spent Kluyveromyces marxianus yeast confirmed the following composition on a dry matter basis: 43.16 % crude protein, 34.56 % total carbohydrate, 7.02 % RNA, and 13.12 % ash content. Carbohydrate components were shown to consist of 20.97 % β-glucan, 9.76 % mannan and 1.21 % chitin while protein characterisation confirmed this yeast’s protein to be a good source of essential amino acids. Total nitrogen, α-amino nitrogen, and descriptive sensory analysis was carried out on yeast extracts derived from yeast autolysis processes carried out at different pH and temperature values. Yeast extract obtained at 50ºC, pH 7.0 was …


Production, Culture, And Representation: An Anthropological Exploration Of Food In Kathmandu, Nepal, Blair Brady Apr 2013

Production, Culture, And Representation: An Anthropological Exploration Of Food In Kathmandu, Nepal, Blair Brady

Independent Study Project (ISP) Collection

This paper explores various food-­‐related narratives, including meat production in Kathmandu, organic farming, the separate experiences of American researchers and American missionaries, "haute cuisine" in a luxury hotel, and a nutritional rehabilitation clinic for malnourished children. Accompanying these narratives are my own experiences that are made communicable through making, or watching food being made. The result of these narratives is a growing picture of the various roles food plays within our lives-­‐ generated by what was communicated by the people who source, cook, and eat food, but also infused by my own observations. Included narratives primarily concern food's ability to …


Growing South Dakota (Spring 2013), College Of Agriculture &. Biological Sciences Apr 2013

Growing South Dakota (Spring 2013), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Food Focus: SDSU Leading National Effort To Promote Healthy Food Choices
[Page] 3 In Stores Now: “Pick It, Try It, Like It” Fruit & Vegetable Campaign
[Page] 4 Coaching Food Entrepreneurs: SDSU Extension Outreach Includes Entrepreneur Workshops
[Page] 5 From South Dakota to South America: College of Agriculture & Biological Science Students Experience Argentine Agriculture
[Page] 6 Spring College News
[Page] 8 Showcasing Ag’s Evolution: SDSU’s Ag Museum Is More Than Just Tractors [Page] 10 Mastering Marketing: Students Host Bull Sale Through Unique Class [Page] 11 Following Pheasants: SDSU Researchers Analyze Nesting Needs
[Page] 12 Faculty Highlights
[Page] …


Sugar Promotes Vegetative Phase Change In Arabidopsis Thaliana By Repressing The Expression Of Mir156a And Mir156c, Li Yang, Mingli Xu, Yeonjong Koo, Jia He, R. Scott Poethig Mar 2013

Sugar Promotes Vegetative Phase Change In Arabidopsis Thaliana By Repressing The Expression Of Mir156a And Mir156c, Li Yang, Mingli Xu, Yeonjong Koo, Jia He, R. Scott Poethig

Faculty Publications

Nutrients shape the growth, maturation, and aging of plants and animals. In plants, the juvenile to adult transition (vegetative phase change) is initiated by a decrease in miR156. In Arabidopsis, we found that exogenous sugar decreased the abundance of miR156, whereas reduced photosynthesis increased the level of this miRNA. This effect was correlated with a change in the timing of vegetative phase change, and was primarily attributable to a change in the expression of two genes, MIR156A and MIR156C, which were found to play dominant roles in this transition. The glucose-induced repression of miR156 was dependent on the …


The Protective Effect Of Antioxidants On Vitamin A Stability In Nonfat Dry Milk During Thermally Accelerated Storage, Amalie Brown Kurzer Mar 2013

The Protective Effect Of Antioxidants On Vitamin A Stability In Nonfat Dry Milk During Thermally Accelerated Storage, Amalie Brown Kurzer

Theses and Dissertations

Two studies were conducted to determine the relative effect of various combinations of antioxidants on vitamin A oxidation and isomerization in nonfat dry milk (NDM). In the first study, one lot of pasteurized unfortified skim milk was divided, fortified with vitamins A and D and one of 11 antioxidant treatments, and spray dried. A control batch from the same lot was also fortified with vitamins A and D and spray dried. Samples were analyzed for total vitamin A bioactivity after zero, one, and two weeks of storage. After two weeks at 50°C, the only NDM samples that did not experience …


Emergency Preparedness In Utah Households With Emphasis On Water And Food Storage Conditions, Stephanie Rae Gerla Mar 2013

Emergency Preparedness In Utah Households With Emphasis On Water And Food Storage Conditions, Stephanie Rae Gerla

Theses and Dissertations

Emergency preparedness steps taken by individuals in Utah households were evaluated in 3 studies. Study 1 evaluated the 2011 landline and cell phone Behavioral Risk Factor Surveillance System Survey and General Preparedness Optional Module results from two states, Louisiana and Utah, to find factors from demographic and medical data that can be used to predict emergency preparedness in individuals. Stepwise logistical regression analysis ascertained the ability of chosen variables to predict individuals' preparedness. The rate of prepared individuals was lower if they were between the ages of 18 to 54 years, when compared to the reference age group of 65 …


College Students' Fruit, Vegetable, And Sugar Sweetened Beverage Intake According To Dinner Group Participation, Erica Nicole Hansen Mar 2013

College Students' Fruit, Vegetable, And Sugar Sweetened Beverage Intake According To Dinner Group Participation, Erica Nicole Hansen

Theses and Dissertations

Objective: To compare the fruit, vegetable, and sugar sweetened beverage intake of college students who do and do not participate in dinner groups. Also, to determine if dinner group participation affects college students' perception of their overall health and adequacy of their fruit and vegetable intake. Design: A quantitative study of college students' fruit, vegetable, and sugar sweetened beverage intake through a one-time online survey. Setting: The survey was accessed via the internet at the time and place of students' convenience. Participants: A sample of 10,000 students was randomly selected from Brigham Young University's population of single students. 3,651 responded …


March 2013 Locations Featured In This Magazine Issue Mar 2013

March 2013 Locations Featured In This Magazine Issue

Agricultural Research Magazine

The Agricultural Research Service has about 100 labs all over the country.

Locations Featured in This Magazine Issue

Davis, California 3 research units ■ 117 employees Corvallis, Oregon 3 research units ■ 127 employees

Vegetable and Forage Crops Research Unit, Prosser, Washington 1 research unit ■ 35 employees

Northwest Irrigation and Soils Research Laboratory, Kimberly, Idaho 1 research unit ■ 34 employees

U.S. Arid-Land Agricultural Research Center, Maricopa, Arizona 3 research units ■ 80 employees

Northern Plains Agricultural Research Laboratory, Sidney, Montana 2 research units ■ 59 employees

Children’s Nutrition Research Center, Houston, Texas 1 research unit ■ 8 employees …


How Does A Mom's Nutrition Affect Her Children's Health?, Robert A. Waterland, Marcia Wood Mar 2013

How Does A Mom's Nutrition Affect Her Children's Health?, Robert A. Waterland, Marcia Wood

Agricultural Research Magazine

In some rural villages of the tiny West African nation of The Gambia, food is generally less available during August and September—the peak of the rainy season—than during a typically dry March through May. Now, a study led by molecular geneticist Robert A. Waterland of the USDA-ARS Children’s Nutrition Research Center in Houston, Texas, has shown that functioning of certain genes in kids conceived during the rainy season differs from that in children conceived during the dry season.

The difference may be explained by a relatively new science referred to as “epigenetics.” The variation appears to be permanent, and in …


Agricultural Research Magazine, March 2013 Mar 2013

Agricultural Research Magazine, March 2013

Agricultural Research Magazine

Table of Contents

4 Weight Loss, Cortisol, and Your Brain: Scientists Explore Connections

6 How Does Mom’s Nutrition Affect Her Children’s Health? Epigenetics May Provide New Insights

8 Enhancing Yogurt With Healthful Fiber From Oats

9 An Atlas for Guatemala, a Tool for Conserving World Crops

10 Linking Animal Behavior to Useful Natural Repellents

12 Cultural Practices To Maintain Soil Quality and Address Climate Change

15 A Desert Shrub’s Crystallized Protein Sheds Light on Photosynthesis

16 Measuring and Managing Impacts of Manure Spills

18 Trickery and Other Methods Explored To Vanquish Potato Cyst Nematodes

20 Measuring the Potential of Switchgrass …


Weight Loss, Cortisol, And Your Brain Scientists Explore Connections, Nancy L. Keim, Kevin D. Laugero, Marcia Wood Mar 2013

Weight Loss, Cortisol, And Your Brain Scientists Explore Connections, Nancy L. Keim, Kevin D. Laugero, Marcia Wood

Agricultural Research Magazine

Americans everywhere are struggling to lose weight—and to keep from putting those lost pounds right back on. For many, it’s discouraging to have their best efforts fail while those of other dieters succeed.

Researchers at the Agricultural Research Service’s Western Human Nutrition Research Center in Davis, California, are conducting studies that may provide new insights into the underlying causes of this disparity in dieting success.

Given America’s obesity epidemic, such research is timely and relevant. The U.S. Centers for Disease Control and Prevention estimates that 35 percent of U.S. adults and 18 percent of kids and adolescents age 6 through …


Enhancing Yogurt With Healthful Fiber From Oats, Mukti Singh, Sanghoon Kim, Marcia Wood Mar 2013

Enhancing Yogurt With Healthful Fiber From Oats, Mukti Singh, Sanghoon Kim, Marcia Wood

Agricultural Research Magazine

Adding about one-quarter teaspoon of a fiber-rich component of oats boosts the nutritional value of low-fat yogurt without noticeably affecting the taste or texture of this increasingly popular dairy food.

Oat fiber is of interest to foodmakers and nutritionists alike. Studies with volunteers have shown that it can lower serum cholesterol, which may help improve heart health.

Agricultural Research Service food technologist Mukti Singh, chemist Sanghoon Kim, and their colleagues experimented with adding fiber-containing oat beta-glucan to what’s known in the dairy industry as “low-fat yogurt mix.” It is made up of low-fat milk and a selection of common, safe-to-eat …


Detecting Errors In Generating A Certificate Of Analysis, Breanne Harlan Mar 2013

Detecting Errors In Generating A Certificate Of Analysis, Breanne Harlan

Dairy Science

Many companies rely on a Certificate of Analysis (COA) to accompany the delivery of their product or material. The COA, required by law, prevents customers from questioning whether a product is safe and ensures manufacturers confirm every product tested passes the Food and Drug Administration’s required specifications. Previous to this project, Kraft Foods Springfield copied their test results for each product from their data warehouse software, called Systems, Applications, and Products onto a Word document to create a COA. This was a systematic approach to detect the possible occurrences of error and reduce the time spent on generating a COA. …


Effect Of Post Manufacture Thermal Dip Treatment On Proteolysis Of Commercial String Cheese During Storage, Melissa Karen Hsu Mar 2013

Effect Of Post Manufacture Thermal Dip Treatment On Proteolysis Of Commercial String Cheese During Storage, Melissa Karen Hsu

Master's Theses

String cheese, a Mozzarella cheese, has the unique ability to string in fibrous strands when pulled apart. Graders judge string cheese by its stringy texture; samples with copious amounts of string are awarded high ratings. But just as the texture of natural cheeses softens with time, the stringy texture of string cheese can diminish with age too.

Age related softening in cheese is due primarily to an important biochemical event known as proteolysis, which is attributed to inherent milk proteinases, residual coagulant activity, and enzymes from the lysis of starter culture microorganisms. It is hypothesized that a post manufacture heat …


Chemotaxonomic Study Of Citrus, Poncirus And Fortunella Genotypes Based On Peel Oil Volatile Compounds - Deciphering The Genetic Origin Of Mangshanyegan (Citrus Nobilis Lauriro), Cuihua Liu, Dong Jiang, Yunjiang Cheng, Xiuxin Deng, Feng Chen, Liu Fang, Zhaocheng Ma, Juan Xu Mar 2013

Chemotaxonomic Study Of Citrus, Poncirus And Fortunella Genotypes Based On Peel Oil Volatile Compounds - Deciphering The Genetic Origin Of Mangshanyegan (Citrus Nobilis Lauriro), Cuihua Liu, Dong Jiang, Yunjiang Cheng, Xiuxin Deng, Feng Chen, Liu Fang, Zhaocheng Ma, Juan Xu

Publications

Volatile profiles yielded from gas chromatography-mass spectrometry (GC-MS) analysis provide abundant information not only for metabolism-related research, but also for chemotaxonomy. To study the chemotaxonomy of Mangshanyegan, its volatile profiles of fruit and leaf and those of 29 other genotypes of Citrus, Poncirus, and Fortunella were subjected to phylogenetic analyses. Results showed that 145 identified (including 64 tentatively identified) and 15 unidentified volatile compounds were detected from their peel oils. The phylogenetic analysis of peel oils based on hierarchical cluster analysis (HCA) demonstrated a good agreement with the Swingle taxonomy system, in which the three genera of Citrus, Poncirus, and …


Consumer Evaluation Of Low Sodium Mozzarella Cheese And Development Of A Novel Method For Evaluating Emotions, Lauren Alyse Collinsworth Mar 2013

Consumer Evaluation Of Low Sodium Mozzarella Cheese And Development Of A Novel Method For Evaluating Emotions, Lauren Alyse Collinsworth

Master's Theses

Mozzarella cheese is currently the highest consumed cheese in the United States. The popularity of mozzarella cheese is typically attributed to the high consumption rates of pizza cheese and string cheese; both of which are low moisture part skim (LMPS) mozzarella cheese. A single serving of LMPS mozzarella cheese contains approximately 8% of the daily value (DV) for sodium, a mineral which is currently consumed in excess among most Americans. On average, one in three Americans has hypertension. This condition is strongly associated with excessive sodium intake, and it is a leading risk factor for cardiovascular disease and stroke in …


Challenges In Testing Genetically Modified Crops For Potential Increases In Endogenous Allergen Expression For Safety, Rakhi Panda, H. Ariyarathna, Plaimein Amnuaycheewa, Afua O. Tetteh, S. N. Pramod, Steve Taylor, B. K. Ballmer-Weber, Richard E. Goodman Feb 2013

Challenges In Testing Genetically Modified Crops For Potential Increases In Endogenous Allergen Expression For Safety, Rakhi Panda, H. Ariyarathna, Plaimein Amnuaycheewa, Afua O. Tetteh, S. N. Pramod, Steve Taylor, B. K. Ballmer-Weber, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

Premarket, genetically modified (GM) plants are assessed for potential risks of food allergy. The major risk would be transfer of a gene encoding an allergen or protein nearly identical to an allergen into a different food source, which can be assessed by specific serum testing. The potential that a newly expressed protein might become an allergen is evaluated based on resistance to digestion in pepsin and abundance in food fractions. If the modified plant is a common allergenic source (e.g. soybean), regulatory guidelines suggest testing for increases in the expression of endogenous allergens. Some regulators request evaluating endogenous allergens for …


Construction And Performance Testing Of A Mixed Mode Solar Food Dryer For Use In Developing Countries, Sean Andrew Foster Jan 2013

Construction And Performance Testing Of A Mixed Mode Solar Food Dryer For Use In Developing Countries, Sean Andrew Foster

Theses and Dissertations

This study details the construction and performance testing of a mixed mode solar dryer using a combination of direct and indirect solar energy to dry food. One major benefit of this dryer design is its construction. It was simple to construct and was made with low cost materials, to make it feasible for use in developing countries. Previous research has identified several design factors that affect performance and efficiency: product loading density, number of trays, position of the absorber, and chimney type. Performance testing showed that chimney air speeds were not affected greatly by modifying the design aspects of the …


Organically Grown Food Provides Health Benefits To Drosophila Melanogaster, Ria Chhabra, Santharam Kolli, Johannes H. Bauer Jan 2013

Organically Grown Food Provides Health Benefits To Drosophila Melanogaster, Ria Chhabra, Santharam Kolli, Johannes H. Bauer

Biosciences Research

The ‘‘organic food’’ market is the fastest growing food sector, yet it is unclear whether organically raised food is nutritionally superior to conventionally grown food and whether consuming organic food bestows health benefits. In order to evaluate potential health benefits of organic foods, we used the well-characterized fruit fly Drosophila melanogaster as a model system. Fruit flies were raised on diets consisting of extracts of either conventionally or organically raised produce (bananas, potatoes, raisins, soy beans). Flies were then subjected to a variety of tests designed to assess overall fly health. Flies raised on diets made from organically grown produce …


The Real Dirt On Biosolids As Soil Amendments, Eton Codling, Ann Perry Jan 2013

The Real Dirt On Biosolids As Soil Amendments, Eton Codling, Ann Perry

Agricultural Research Magazine

The U.S. Environmental Protection Agency (EPA) estimates that more than 60 percent of dry biosolids—treated wastewater solids that can be recycled or stored—are applied to land, composted, or used to cover landfills. The biosolids have been processed to kill pathogens, and EPA strictly regulates biosolid use to ensure the materials don’t harm the environment, human health, or animal health.

Farmers who follow pre- and post-application management regulations can obtain permits to use biosolids for amending fields where food and feed crops are grown. Now, research by an Agricultural Research Service scientist is helping to clarify the long-term effects that biosolid …


With Hot Air Treatment, Bacteria Fly The Coop, Rosalie Marion Bliss, Mark E. Berrang Jan 2013

With Hot Air Treatment, Bacteria Fly The Coop, Rosalie Marion Bliss, Mark E. Berrang

Agricultural Research Magazine

While being transported in hauling coops on trucks, poultry that have been colonized with bacteria such as Campylobacter can contaminate, through fecal shedding, pathogen-free poultry. Those pathogens can also be passed on to the next group of birds during the next trip, and so forth, unless the cycle is broken.

That’s where Agricultural Research Service microbiologists Mark Berrang and Richard Meinersmann and colleague Charles Hofacre at the University of Georgia in Athens come in. The team has reported a treatment that reduces poultry cross-contamination from transport-cage flooring.

Campylobacter are foodborne pathogens that can be present in raw or undercooked poultry. …


Going Beyond The Barnyard To Stop Stable Flies, David B. Taylor, J.J. Zhu, Sandra Avant Jan 2013

Going Beyond The Barnyard To Stop Stable Flies, David B. Taylor, J.J. Zhu, Sandra Avant

Agricultural Research Magazine

Livestock producers may not be able to see the difference between stable flies and other flies at a distance, but they can definitely see the stable flies’ effect on their cattle as the animals stop grazing and bunch together to minimize the number of bites they’re getting.

Stable flies are among the most important arthropod pests of cattle in the United States. Their painful bites can reduce milk production in dairy cows, decrease weight gain in beef cattle, and reduce feed efficiency.

Generally, insecticide sprays are used to help keep stable flies off animals, especially their legs, where the flies …


Agricultural Research Magazine, January 2013 Jan 2013

Agricultural Research Magazine, January 2013

Agricultural Research Magazine

Table of Contents

4 Helping Citrus Growers Deal With a Nasty Invader

7 Orange Juice Amino Acids May Reveal Secrets to Quelling Citrus Greening Disease

8 Drainage Ditch Research Reveals Opportunities for Cleaning Up Runoff

11 Crop-Friendly Bacteria Tapped To Battle Fungal Marauders

12 Advantages of Understanding the Lady Beetle Diet

13 New Uses for Sugar Beet Pulp

14 Going Beyond the Barnyard To Stop Stable Flies

16 The Real Dirt on Biosolids as Soil Amendments

17 With Hot Air Treatment, Bacteria Fly the Coop

18 ARS Works Toward Control of Brown Marmorated Stink Bug

21 New Technology for Harvesting …


Genetic And Physiological Responses Of Bifidobacterium Animalis Subsp. Lactis To Hydrogen Peroxide Stress, T. S. Oberg, Robert E. Ward, J. L. Steele, Jeff Broadbent Jan 2013

Genetic And Physiological Responses Of Bifidobacterium Animalis Subsp. Lactis To Hydrogen Peroxide Stress, T. S. Oberg, Robert E. Ward, J. L. Steele, Jeff Broadbent

Nutrition, Dietetics, and Food Sciences Faculty Publications

Consumer interest in probiotic bifidobacteria is increasing, but industry efforts to secure high cell viability in foods is determined by these anaerobes’ sensitivity to oxidative stress. To address this limitation, we investigated genetic and physiological responses of two fully sequenced Bifidobacterium animalis subsp. lactis strains, BL-04 and DSM 10140, to hydrogen peroxide (H2O2) stress. Although the genome sequences for these strains are highly clonal, prior work showed they differ in both intrinsic and inducible H2O2 resistance. Transcriptome analysis of early stationary phase cells exposed to a sub-lethal H2O2 concentration detected …


Intellectual Property And Opportunities For Food Security In The Philippines, Jane Payumo, Howard Grimes, Antonio Alfonso, Stanley P. Kowalski, Keith Jones, Karim Maredia, Rodolfo Estigoy Jan 2013

Intellectual Property And Opportunities For Food Security In The Philippines, Jane Payumo, Howard Grimes, Antonio Alfonso, Stanley P. Kowalski, Keith Jones, Karim Maredia, Rodolfo Estigoy

Law Faculty Scholarship

By 2050, the Philippine population is projected to increase by as much as 41 percent, from 99.9 million to nearly 153 million people. Producing enough food for such an expanding population and achieving food security remain a challenge for the Philippine government. This paper argued that intellectual property rights (IPR) can play a key role in achieving the nation’s current goal to be food-secure and provided examples to illustrate that the presence of sound intellectual property (IP) helps foster research, development, and deployment of agricultural innovations. This paper also offered key recommendations about how the IP system can be further …


Food Intake Patterns And Plate Waste Among Community Meal Center Guests Show Room For Improvement, Marjorie Freedman, Catherine Bartoli Jan 2013

Food Intake Patterns And Plate Waste Among Community Meal Center Guests Show Room For Improvement, Marjorie Freedman, Catherine Bartoli

Faculty Publications

Food insecure individuals often consume nutritionally inadequate diets. Using a weighted plate waste analysis, we examined whether adult guests of 2 independently operated meal centers were served and consumed Food and Drug Administration–recommended serving sizes (reference amount customarily consumed; RACC) of protein, starch, fruit, vegetables, and bread for the dinner meal. In both centers, guests were served and consumed more than 100% of RACC for protein. Regardless of amount served and independent of whether guests took seconds, consumption of fruit and vegetables was less than RACC. Larger servings of vegetables, but not of protein or starch, resulted in more plate …


The Effect Of Freezing As A Storage Method On Anthocyanin Concentration In Blueberries, Marin E. Plumb Jan 2013

The Effect Of Freezing As A Storage Method On Anthocyanin Concentration In Blueberries, Marin E. Plumb

Undergraduate Honors Papers

Blueberries are rich in a water-soluble class of pigments known as anthocyanins which are known antioxidants. Antioxidants help prevent many diseases by stabilizing free radicals, but are prone to losses in food during storage. The goal of this study was to test the effect of freezing as a storage method on anthocyanin concentration and antioxidant activity over time. Blueberries from Canada and Argentina were frozen for up to 5 months and periodically tested for anthocyanin concentration and antioxidant activity. Anthocyanins were extracted using a mixture of methanol, acetic acid, and water, and evaporation under vacuum. They were separated using column …