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New Functionally-Enhanced Soy Proteins As Food Ingredients With Anti-Viral Activity, Aizhan Sabirzhanovna Turmagambetova, Nadezhda Sergeevna Sokolova, Andrey Pavlinovich Bogoyavlenskiy, Vladimir Eleazvich Berezin, Mary Ann Lila, Diana M. Cheng, Vyacheslav Dushenkov Mar 2015

New Functionally-Enhanced Soy Proteins As Food Ingredients With Anti-Viral Activity, Aizhan Sabirzhanovna Turmagambetova, Nadezhda Sergeevna Sokolova, Andrey Pavlinovich Bogoyavlenskiy, Vladimir Eleazvich Berezin, Mary Ann Lila, Diana M. Cheng, Vyacheslav Dushenkov

Publications and Research

Respiratory viruses are a major public health problem because of their prevalence and high morbidity rate leading to considerable social and economic implications. Cranberry has therapeutic potential attributed to a comprehensive list of phytochemicals including anthocyanins, flavonols, and unique A-type proanthocyanidins. Soy flavonoids, including isoflavones, have demonstrated anti-viral effects in vitro and in vivo. Recently, it was demonstrated that edible proteins can efficiently sorb and concentrate cranberry polyphenols, including anthocyanins and proanthocyanins, providing greatly stabilized matrices suitable for food products. The combination of cranberry and soy phytoactives may be an effective dietary anti-viral resource. Anti-viral properties of both cranberry juice-enriched …


Stability Of Selected B Vitamins In Thermally-Treated Pinto Beans, Virginia Anne West Mar 2015

Stability Of Selected B Vitamins In Thermally-Treated Pinto Beans, Virginia Anne West

Theses and Dissertations

Beans are a commonly consumed food and a staple in many regions worldwide. Pinto beans (Phaseolus vulgaris), categorized as legumes, are dried seeds from plants and are high in protein, carbohydrate and fiber, and low in fat. They are also a good source of various minerals and well as thiamin, riboflavin, niacin, vitamin B6, and folate Beans are typically soaked and thermally processed before consumption. Different processing methods can impact the composition of beans. The purpose of this study was to examine the effect of thermal treatments on vitamin concentration in pinto beans. Beans were simmered, canned, dried-flaked, or dried-extruded, …


Change Pattern Of Nutrition Composition During Germination Of Three Kind Of Soybean, Wang Hongtao, Chen Cheng, Yu Fang, Chen Baohong Feb 2015

Change Pattern Of Nutrition Composition During Germination Of Three Kind Of Soybean, Wang Hongtao, Chen Cheng, Yu Fang, Chen Baohong

Food and Machinery

The nutrient compositions’ change of green beans, black beans, soybean were studied during sprouting in order to provide experimental basis for the sprouting soybean food production and processing. Control soybean germination conditions by using a certain temperature and humidity, regular sampling and assay sprouted soybean’s crude protein, crude fat, reducing sugar, Vc and isoflavone’s content. The results show that: the protein content of green beans, soybean and black beans after seven days’ germination increased by 24.03%, 24.28% and 22.88% respectively than after one day’s germination, the reducing sugar content increased by 129.06%, 127.17% and 125.73% respectively; the Vc content increased …


Analysis On Feasibility Of Detection Of Melamine In Liquid Milk Based On Near-Infrared Spectroscopy, Cheng Wenyu, Guan Xiao, Liu Jing Feb 2015

Analysis On Feasibility Of Detection Of Melamine In Liquid Milk Based On Near-Infrared Spectroscopy, Cheng Wenyu, Guan Xiao, Liu Jing

Food and Machinery

The reasonability using near-infrared spectra (NIRS) for detection of melamine in liquid milk were studied. NIRS of samples were obtained by Fourier Transform Infrared Spectroscopy(FTIR) spectrometer, samples of liquid milk with adding melamine were analyzed while characteristic signals were extracted by wavelet transform and principal component analysis. Quantitative analysis was taken by General the Regression Neural Network(GRNN) for the diffuse reflectance spectroscopy of liquid milk adding melamine first. The result is not satisfactory enough that when concentration of melamine is lower than 0.05%, the R2 of predict model is only 0.567. Qualitative discrimination by support vector machine was carried …


Determination Of Twelve Elements In Jujube By Inductively Coupled Plasma Atomic Emission Spectrometry (Icp—Aes), Chen Kai, Li Jinyu, Li Qiong, Li Huanrong Feb 2015

Determination Of Twelve Elements In Jujube By Inductively Coupled Plasma Atomic Emission Spectrometry (Icp—Aes), Chen Kai, Li Jinyu, Li Qiong, Li Huanrong

Food and Machinery

A method was developed using ICP-AES for the simultaneous determination on the content of trace heavy metals including Na, Mg, Al, K, etc in 3 spieces of jujube of Xin Jiang district. The correlation index of method was greater than 0.998; Determination of elements in addition to the individual value, RSD is less than 5%. The results showed that: xinjiang jujube had rich mineral elements content, which especially in K (>12 277 μg/g), Ca (>785 μg/g), Mg (> 646 μg/g), Na (425 μg/g), Al (>179 μg/g), Fe (>22 μg/g) content is higher, the K and Fe content of Aksu jun …


Study On Preparation Of Body Wall Hydrolysate Of Starfish Asterina Pectinifera And Antioxidant Activity Detection, Li Yubo, Ji Xiaolin, Liu Shan, Zha Yue, Xin Qiuyan, Qi Hang Feb 2015

Study On Preparation Of Body Wall Hydrolysate Of Starfish Asterina Pectinifera And Antioxidant Activity Detection, Li Yubo, Ji Xiaolin, Liu Shan, Zha Yue, Xin Qiuyan, Qi Hang

Food and Machinery

Hydrolysate was extracted from starfish Asterina pectinifera, the starfish Asterina pectiniferas were hydrolyzed with pepsin, neutrase and trypsin under their optimal conditions to obtain the hydrolysate, and the antioxidant activity of hydrolysate was detected by using electron spin resonance(ESR) spectroscopy The results indicated that the DPPH radical and hydroxyl radical scavenged ability increased with the increasing concentration of hydrolysate; at the concentration of 20 mg/mL, the clearance rate of pepsin group, neutrase group and trypsin group on DPPH radical were(5225±014)%, (6830±037)% and (4627±047)% respectively, and the rate of hydroxyl radical were(7261±017)%, (8182±079)% and (5677±028)% respectively.


Study On Antitumor Activities Of Five Kinds Of Wild Edible Mushrooms In Changbai Mountain, Li Xue, Li Pengfei, Li Xiaodong, Yu Ting, Jin Qiankun, Cui Chengbi Feb 2015

Study On Antitumor Activities Of Five Kinds Of Wild Edible Mushrooms In Changbai Mountain, Li Xue, Li Pengfei, Li Xiaodong, Yu Ting, Jin Qiankun, Cui Chengbi

Food and Machinery

To study the anti-tumor activities of wild edible mushrooms of the Changbai Mountain, the experiment selected Ganoderma Lucidum, Inonotus Obliquus, Phellinus, Hericium Erinaceus, Lentinula Edodes five kinds of Changbai Mountain wild edible mushrooms as materials, the experiment was divided into in vitro and in vivo study. The inhibition effect of tumor cells was tested by MTT method, and the inhibition ratio of mouse sarcoma S-180 was calculated in vivo study. The results showed that Ganoderma Lucidum has the highest inhibition ratio on the HeLa and HepG2 cell lines. Phellinus has the highest inhibition rate on MCF-7 cell line, Hericium Erinaceus …


Analysis Of Nutritional And Bioactive Components Of Solid-State Fermented Substrate Of Isaria Cicadae, Yu Shijun, Chai Xinyi, Fan Meizhen Feb 2015

Analysis Of Nutritional And Bioactive Components Of Solid-State Fermented Substrate Of Isaria Cicadae, Yu Shijun, Chai Xinyi, Fan Meizhen

Food and Machinery

In the present work, the nutritional and bioactive components of the solid-state fermented substrate of I. cicadae were studied. And the results indicated that the contents of protein, total sugar and fat acid were 16.17%, 38.87% and 1.43%, respectively. The unsaturated fat acid content was 1.18%. The amino acid profile was analyzed and lysine was the first restrictive amino acid in the FAO/WHO model. The contents of vitamin E and C were 1 833 mg/kg and 239 mg/kg, respectively. Furthermore, the fermented substrate contained adenosine, mannitol and polysaccharide. This work provided basic information for the further development of Fermented Substrate …


Preparation Of Germinated Soybean Milk With Household Soymilk Machine, Jin Zixin, Yang Ruijin, Zhu Shengbo, Liu Xinjuan, Zhang Wenbin Feb 2015

Preparation Of Germinated Soybean Milk With Household Soymilk Machine, Jin Zixin, Yang Ruijin, Zhu Shengbo, Liu Xinjuan, Zhang Wenbin

Food and Machinery

The objectives of this study were to optimize pulverizing and boiling process for germinated soybean milk. Pulverizing temperature and boiling time were explored through single-factor experiments and the optimal temperature was figured out as 70 ℃ and boiling time as 4 min.With optimized process, content of γ-aminobutyric acid, total freeamino acids, essential freeamino acids in the germinated soybean based milk were 1.36 mg/100 g, 0.455 g/100 g, and 0.219 g/100 g, respectively. Compared with dry soybean milk, the above contents were 3.68, 1.34, 8.87 folds increased respectively. Despite that the trypsin inhibitor activity (TIA) was 1.27 times that of dry …


Optimization On Technology Of Potato Yogurt, Wu Jianmin, Liu Shaojun, Shao Songlin Feb 2015

Optimization On Technology Of Potato Yogurt, Wu Jianmin, Liu Shaojun, Shao Songlin

Food and Machinery

To reseach technology of the potato yogurt,the technology and formulation of potato yogurt inoculation of the ferment,fermentation time,temperature and other parameters were studied. The optimum technological conditions of potato yogurt were optimized.The optimum formulation was as followed: potato juice to milk 20∶70(V∶V), sugar 4%(m/V), Starter cultures 8%(V/V), ferment time 6 h.


Processing Technology Of Rosa Sterilis Yoghourt Buccal Tablets, Yan Xiaoyue, Liu Xing, Li Yongfu, Chen Qinfang, Zhong Yaoguang, Wang Zhengwu Feb 2015

Processing Technology Of Rosa Sterilis Yoghourt Buccal Tablets, Yan Xiaoyue, Liu Xing, Li Yongfu, Chen Qinfang, Zhong Yaoguang, Wang Zhengwu

Food and Machinery

Rosa sterilis, sericin peptides and skim milk were used as raw materials to produce yoghourt buccal tablets of the ratio of raw materials and preparation conditions, which could enrich yoghourt buccal tablets varieties in the market and improve their nutritional value. The results showed that the optimal co-fermentation time was 4 hours, the best ratio of Rosa sterilis, xylitol and dried skim milk 2∶5∶10 and the content of magnesium stearate 2%. The yoghourt buccal tablets were nutritive and suitable sour and sweet, rich in yogurt flavor and the fruital of Rosa sterilis, whose nutritional and hygienic indexes could meet the …


Development Andchallenge Of 3d Print In The Food Industry, Li Guangling Feb 2015

Development Andchallenge Of 3d Print In The Food Industry, Li Guangling

Food and Machinery

With the rapid development of 3D printing technology, its applications expand into the field of architecture, industrial design and orthopedic from the traditional mold manufacturing. The cost reduction is benefited from the development of opening hardware technology. In the food industry, 3D food printing will have a profound impact on shortage of food supply, individual needs. But there are also many challenges in material limitations, taste, safety, cost and psychological disorders which 3D food printing must face.


Advances In Heat And Mass Transfer Models Of Microwave Freeze-Drying, Yan Shasha, Duan Xu, Ren Guangyue, Liu Wei Feb 2015

Advances In Heat And Mass Transfer Models Of Microwave Freeze-Drying, Yan Shasha, Duan Xu, Ren Guangyue, Liu Wei

Food and Machinery

Microwave freeze-drying is a rapid dehydration technique that can be applied to certain foods, particularly to seafoods, and fruits and vegetables. MFD involves much less drying time and energy consumption than conventional freeze-drying methods, and also improves product quality. The article presents the principles of microwave freeze-drying, the research progress of heat and mass transfer mathematical simulation at home and abroad, the detection technology online, and the dielectric properties of materials taken into account in the drying process simulation. After analyzing the problems, the trend of numerical simulation of freeze-drying is predicted.


Research Progress Of Health Functions Of Wheat Bran, Du Zhenya, Chen Fusheng, Bu Guanhao Feb 2015

Research Progress Of Health Functions Of Wheat Bran, Du Zhenya, Chen Fusheng, Bu Guanhao

Food and Machinery

As one of the important dietary fiber source, wheat bran contains various active polysaccharides, phenolic compounds and others physiologically active substances, which plays an important role in human health. Mainly introduced and prospected the function of wheat bran in relieving constipation, colon cancer, diabetes and other.


Progress On Aflatoxin Removing Technique, Cao Ming, Fan Mingtao Feb 2015

Progress On Aflatoxin Removing Technique, Cao Ming, Fan Mingtao

Food and Machinery

Aflatoxin B1 is the best toxicity and carcinogenicity in the aflatoxin, and it is listed inⅠA level carcinogens by international tumor research institutes. Recently, it becomes the hot spot on how to get a method with high efficient, safe for aflatoxin removing. Meanwhile, summarizes the technologies for aflatoxin removing by physical, chemical and biological methods in this paper, including physical adsorption, extrusion, irradiation, microwave degradation, ozone degradation, ammonia fumigation, biological adsorption, biodegradable etc.. Moreover, the development of aflatoxin B1 removing is also prospected.


Teaching Reform And Practice Of Food Nutrition Based On Constructivism, Nie Xiaohua, Sun Peilong, He Jinzhe, Meng Xianghe Feb 2015

Teaching Reform And Practice Of Food Nutrition Based On Constructivism, Nie Xiaohua, Sun Peilong, He Jinzhe, Meng Xianghe

Food and Machinery

Food nutrition is an important foundation course of food science and engineering. In order to improve the teaching quality, the teaching reform and practice was developed on the basis of the content, style and practice by using constructivism theory. It stimulated the students’ subjective initiative and practice competence, and. good teaching effect has been received.


Beth Hanna, Beth Hanna, Institute Of Child Nutrition. Child Nutrition Archives. Feb 2015

Beth Hanna, Beth Hanna, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Beth Hanna, PhD, RD, LD, SNS, is a registered dietician who received her PhD in Food and Lodging Management from Iowa State University.

Her work experience includes serving as Director of Nutrition Services for West Des Moines Community Schools where she managed all aspects of the school nutrition program in compliance with federal and state regulations.

Dr. Hanna also served as an instructor with Iowa State University and a consultant for the Iowa Department of Education, Bureau of Food and Nutrition. She reviewed child nutrition programs and offered specialized support in equipment layout and design.

In addition to being a …


Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt Feb 2015

Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt

Department of Civil and Environmental Engineering: Faculty Publications

The use of wastewater for irrigation may introduce antimicrobials and human pathogens into the food supply through vegetative uptake. The objective of this study was to investigate the uptake of three antimicrobials and Salmonella in two lettuce cultivars. After repeated subirrigation with synthetic wastewater, lettuce leaves and soil were collected at three sequential harvests. The internalization frequency of Salmonella in lettuce was low. A soil horizon-influenced Salmonella concentration gradient was determined with concentrations in bottom soil 2 log CFU/g higher than in top soil. Lincomycin and sulfamethoxazole were recovered from lettuce leaves at concentrations as high as 822 ng/g and …


Presentations And Posters From The 2015 Research That Matters Sustainable Food Conference: People, Policy, & Practice, Various Authors Jan 2015

Presentations And Posters From The 2015 Research That Matters Sustainable Food Conference: People, Policy, & Practice, Various Authors

Food and Gardening Working Group

The University of South Florida Sustainable Food Conference: People, Policy, & Practice brought together food researchers from USF and the larger Tampa Bay community to share ideas and findings that will pave the path toward more sustainable and resilient communities. It featured research papers and presentations from all disciplines and methodologies addressing issues concerning food production, distribution, retail, and/or consumption.


Microbial Safety And Quality Of Fresh Herbs From Los Angeles, Orange County, And Seattle Farmers’ Markets, Donna J. Levy, Nicola K. Beck, Alexandra L. Kossick, Taylor Patti, J. Scott Meschke, Melissa Calicchia, Rosalee S. Hellberg Jan 2015

Microbial Safety And Quality Of Fresh Herbs From Los Angeles, Orange County, And Seattle Farmers’ Markets, Donna J. Levy, Nicola K. Beck, Alexandra L. Kossick, Taylor Patti, J. Scott Meschke, Melissa Calicchia, Rosalee S. Hellberg

Food Science Faculty Articles and Research

BACKGROUND: Farmers' markets have been growing in popularity in the United States, but the microbial quality and safety of the food sold at these markets is currently unknown. The purpose of this study was to assess the microbial safety and quality of fresh basil, parsley and cilantro sold at farmers' markets in the Los Angeles, Orange County and greater Seattle areas.

RESULTS: A total of 133 samples (52 basil, 41 cilantro and 40 parsley) were collected from 13 different farmers' markets and tested for Salmonella and generic Escherichia coli. One sample (parsley) was confirmed positive for Salmonella and 24.1% of …


Message From The Editorial Board, John R. Clark Jan 2015

Message From The Editorial Board, John R. Clark

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Instructions For Authors, Discovery Editors Jan 2015

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Impact Of Algae Supplemented Diets Combined With Antioxidants On The Nutritional Profile, Quality Attributes, And Storage Stability Of Chicken Breast Meat, Rebecca G. Norcross Jan 2015

Impact Of Algae Supplemented Diets Combined With Antioxidants On The Nutritional Profile, Quality Attributes, And Storage Stability Of Chicken Breast Meat, Rebecca G. Norcross

Theses and Dissertations--Animal and Food Sciences

Consumers’ demands for ω-3 polyunsaturated fatty acids (PUFAs) are at all-time high. Algae, a common source of PUFAs, and antioxidants are both used as supplements in livestock feeds, are known to affect the overall quality of meat. To implement PUFA deposits into broiler meat, this study evaluated combining antioxidants and algae in broiler feed to enhance the breast meat quality. Broilers were fed diets supplemented with 50 IU Vitamin E or 200 g/ton EconomasE (EcoE, an antioxidant pack) plus 10 IU Vitamin E, with or without 0.5% algae extract (SP-1). The feed oil was partially oxidized soybean oil (POV: 86 …


Antimicrobial Efficacy Of Natural Bioactive Compounds And High Pressure Processing Against Potential Pathogens In Infant Foods, Hayriye Cetin-Karaca Jan 2015

Antimicrobial Efficacy Of Natural Bioactive Compounds And High Pressure Processing Against Potential Pathogens In Infant Foods, Hayriye Cetin-Karaca

Theses and Dissertations--Animal and Food Sciences

This study investigated the antimicrobial efficacy of bioactive plant compounds along with high pressure processing (HPP) against pathogens Bacillus cereus and Cronobacter sakazakii in infant formula and infant rice cereal. The influence of these applications on antimicrobial activity, shelf-life and sensory attributes of infant foods were examined.

Trans-cinnamaldehyde (TC), (-)-Epigallocatechin gallate (EGCG) and [10]-Gingerol (GI) were incorporated (0.05%) in infant rice cereal reconstituted with infant formula. The cereal was inoculated with either B. cereus (ATCC 14579) or B. cereus spores (107-108 log CFU g-1). All the samples were stored at 7, 23 or 37°C for …


A Dna Mini-Barcoding System For Authentication Of Processed Fish Products, Shadi Shokralla, Rosalee S. Hellberg, Sara M. Handy, Ian King, Mehrdad Hajibabaei Jan 2015

A Dna Mini-Barcoding System For Authentication Of Processed Fish Products, Shadi Shokralla, Rosalee S. Hellberg, Sara M. Handy, Ian King, Mehrdad Hajibabaei

Food Science Faculty Articles and Research

Species substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences—mini-barcodes—for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species commonly used in processed fish products in North America. Six mini-barcode primer pairs targeting short (127–314 bp) fragments of the cytochrome c oxidase I (CO1) DNA barcode region were developed by examining over 8,000 DNA barcodes from species in the U.S. Food and Drug Administration …


Increasing The Nutritional Value Of Canola Meal Via Fungal Bioprocessing, Jason R. Croat Jan 2015

Increasing The Nutritional Value Of Canola Meal Via Fungal Bioprocessing, Jason R. Croat

Electronic Theses and Dissertations

The main limitation of meals from canola and other Brassica spp. is the presence of glucosinolates (GLS), which are anti-nutritional and can even be toxic at high ingestion levels. Furthermore, large amounts of GLS can reduce palatability for livestock and thus reduce intake and growth rates. For this reason canola was bred to contain lower levels of GLS (<30 >μmol/g) and erucic acid (< 2%). However, feed inclusion rates are still limited to ~30%, and this reduces the value of canola meal. The goal of this thesis was to optimize a pretreatment and fungal conversion process to enhance the nutritional value of canola meal. Various combinations of physical/chemical pretreatments, fungal cultures, and incubation methods were investigated to metabolize GLS into cell mass, CO2, or other non-toxic components. These treatments also served to hydrolyze canola meal fiber into carbohydrates which were then metabolized by the fungi into single cell protein. Solid-state incubation conditions were initially tested, since filamentous fungi are well adapted to grow at lower moisture levels, and this would potentially reduce contamination issues with bacteria. Flask trials were performed using 50% moisture, hexane extracted (HE) or cold pressed (CP) canola meal, with incubation for 168 h at 30ºC. On HE canola meal Trichoderma reesei (NRRL-3653) achieved the greatest increase in protein content (23%), while having the lowest residual levels of sugar (8% w/w) and GLS (0.4 μM/g). On CP canola meal T. reesei (NRRL-3653), Aureobasidium pullulans (NRRL-58522), and …


Contents, Discovery Editors Jan 2015

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 16 2015, Several Authors Jan 2015

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 16 2015, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Non-Dairy Probiotic Products, Nissreen Abu-Ghannam, Gaurav Rajauria Jan 2015

Non-Dairy Probiotic Products, Nissreen Abu-Ghannam, Gaurav Rajauria

Books/Book Chapters/ Proceedings

The term probiotic was technically defined as “live microorganisms which upon ingestion in certain numbers exert health benefits beyond inherent nutrition” (FAO/ WHO 2001). This definition requires that the microorganisms must be alive and present in high numbers, generally more than 109 cells per daily ingested dose. Probiotic food products are considered as functional foods which are defined to contain health-promoting components beyond traditional nutrients and the addition of probiotic cultures is one approach in which foods could be modified to become functional.


Detection Of Beneficial Microbiota In Mouse Colon, Aranya Linpisanl Jan 2015

Detection Of Beneficial Microbiota In Mouse Colon, Aranya Linpisanl

Wayne State University Theses

Lactic acid bacteria (LAB) and Bifidobacterium are the most common types of microbes used as probiotics. They are present in the human gastrointestinal tract and have a significant influence on our health and well-being. Microbiota plays an important role in host metabolism and provides a natural defense mechanism against invading pathogens. This experiment was focusing on establish a method to detect the gastrointestinal tract microbiota, either by fecal or colonic tissue DNA extraction.

The experiment comparing 2 types of DNA extraction; ZR Fecal kit and DNAzol direct. DNAzol direct was easy to use but was not suitable for long term …