Digital Commons Network^™

Bridging The Food Gap: Founding And Sustaining A Food Recovery Network Chapter At Wku, Elaine Losekamp

Mahurin Honors College Capstone Experience/Thesis Projects

Food waste is a pervasive global issue with many environmental and social repercussions. While about one-third of all food produced for human consumption goes to waste, many people in the United States and the world are affected by food insecurity. Food recovery, the process of rescuing edible food that would otherwise go to waste and delivering to hungry people, is an effective solution for both food waste and food insecurity. The author of this capstone created a food recovery program at Western Kentucky University (WKU) in January 2019 and has grown the program’s scope and impact since that time. This …

Formation Of Escherichia Coli O157: H7 Persister Cells In The Lettuce Phyllosphere And Application Of Differential Equation Models To Predict Their Prevalence On Lettuce Plants In The Field, Daniel S. Munther, Michelle Q. Carter, Claude V. Aldric, Renata Ivanek, Maria T. Brandl

Mathematics and Statistics Faculty Publications

American Society for Microbiology. Escherichia coli O157:H7 (EcO157) infections have been recurrently associated with produce. The physiological state of EcO157 cells surviving the many stresses encountered on plants is poorly understood. EcO157 populations on plants in the field generally follow a biphasic decay in which small subpopulations survive over longer periods of time. We hypothesized that these subpopulations include persister cells, known as cells in a transient dormant state that arise through phenotypic variation in a clonal population. Using three experimental regimes (with growing, stationary at carrying capacity, and decaying populations), we measured the persister cell fractions in culturable EcO157 …

Natural Products For The Management Of Cardiovascular Diseases, Maha Salama, Shahira Mohammed Ezzat, Mohamed Salem

Pharmacy

Cardiovascular diseases constitute a serious public health problem. It is estimated that they are responsible for nearly 30% of world mortality. Regardless of the developments in the diagnosis and management of cardiovascular diseases, their incidence rate remains increasing. Therefore, newlines of drugs are needed to manage the expanding population of patients with cardiovascular diseases. Even though the most common existing treatments for cardiovascular diseases are synthetic molecules, natural compounds, of different chemical classes, are also being tested. Medicinal plants have been employed in the treatment of some cardiovascular diseases such as congestive heart failure and hypertension many centuries ago. Recently, …

Sampling The Local Fare: Fishes At The Sam Israel House Pit (45gr76), Soap Lake, Washington, Adam Fruge

All Master's Theses

The Sam Israel site is a precontact archaeological complex with numerous fish bones at the north end of Soap Lake, Washington. Excavated in 1976, the fish remains recovered from there were never fully analyzed prior to this research. Since this inland Columbia Plateau site had thousands of fish bones, it contained untapped potential for our understanding of ancient local fish procurement. As such, I conducted a detailed analysis of 2,862 fish bone specimens from the Sam Israel House Pit locus to: study a larger sample of fish bones in greater detail than was done before; compare the distribution of fishes …

Effects Of Hydrocolloids On Pasting Behavior And Gelling Properties Of Banana Flour And Its Application In Bread, Rosalin Herman

Chulalongkorn University Theses and Dissertations (Chula ETD)

Bananas have a high potential in becoming a future staple crop due to its rapid growth rate, short harvest time, and high starch content. However, banana flour is highly retrograded which is its major limitation. The objective of this study was to study the effects of selected hydrocolloids on the pasting and gelling properties as related to the gelatinization and retrogradation of banana flour as well as its application in bread as a wheat flour substitute. Xanthan gum (XG), gum arabic (GA), hydroxypropyl methylcellulose (HPMC), and guar gum (GG) were added to banana flour at different concentrations (0.5, 1.0, and …

Is Vegan Confectionary Sold In The United Kingdom Safe For Milk Allergy Sufferers?, Faye Harrison

Electronic Theses and Dissertations

Background
Milk allergy is the most common childhood allergy. Reading food labels is important to prevent an allergic reaction. By United Kingdom (UK) law, milk needs to be listed on the food label if it used as an ingredient. Products can be called ‘free from’ milk if manufacturers have undergone rigorous process and testing to ensure it is not contaminated with milk. Precautionary Allergen Labelling (PAL) like ‘may contain milk’ statements are unregulated and can be used at manufacturer’s discretion. Similarly, ‘Vegan’ products are not regulated by law and appear to be on the increase. Objective
The study aimed to …

What The Covid-19 Crisis Is Telling Humanity, David Wiebers, Valery Feigin

Animal Sentience

The planet is in a global health emergency exacting enormous medical and economic tolls. It is imperative for us as a society and species to focus and reflect deeply upon what this and other related human health crises are telling us about our role in these increasingly frequent events and about what we can do to prevent them in the future.

Cause: It is human behavior that is responsible for the vast majority of zoonotic diseases that jump the species barrier from animals to humans: (1) hunting, capture, and sale of wild animals for human consumption, particularly in live-animal markets; …

Innovation In Meat Production: A Problem And An Opportunity, Christopher J. Bryant

Animal Sentience

Innovation in meat production has enabled modern humans to inflict far greater harm on animals, the environment, and public health than was possible just a few decades ago. Wiebers & Feigin aptly express the urgency with which these issues must be addressed. Those advocating for animals on moral grounds face resistance from omnivores citing taste, price and convenience. Further innovation in meat production (plant-based and cultured meat) will enable us to preserve the experience of eating meat whilst phasing out the many problems caused by industrial animal farming.

Determining The Age Of Spoiled Milk From Dried Films Using Attenuated Reflection Fourier Transform Infrared (Atr Ft-Ir) Spectroscopy, Zack Richardson, David Perezguaita, Kamila Kochan, Bayden Wood

Articles

Milk spoilage is an inevitable occurrence, which generates waste and can result in food poisoning. When milk spoils, the off-flavor and curdling are due to excessive proliferation of various bacteria which causes pH changes. Time, temperature, environment,and previous handling practice all affects the spoilage rate. There is a need for a fast reliable and accurate method that can identify in situearly spoilage of milk. Here we show the ability of attenuated total reflectionFourier transformed infrared spectroscopy(ATR FT-IR) in conjunction with multivariate data analysis to predict the age of milk. We found that dried films vastly increased the absorbance of important …

The Trait Repertoire Enabling Cyanobacteria To Bloom Assessed Through Comparative Genomic Complexity And Metatranscriptomics, Huansheng Cao, Yohei Shimura, Morgan M. Steffen, Zhou Yang, Jingrang Lu, Allen Joel, Landon Jenkins, Masanobu Kawachi, Yabin Yin, Ferran Garcia-Pichel

Department of Food Science and Technology: Faculty Publications

Water bloom development due to eutrophication constitutes a case of niche specialization among planktonic cyanobacteria, but the genomic repertoire allowing bloom formation in only some species has not been fully characterized. We posited that the habitat relevance of a trait begets its underlying genomic complexity, so that traits within the repertoire would be differentially more complex in species successfully thriving in that habitat than in close species that cannot. To test this for the case of bloom-forming cyanobacteria, we curated 17 potentially relevant query metabolic pathways and five core pathways selected according to existing ecophysiological literature. The available 113 genomes …

Identification Of Simplified Microbial Communities That Inhibit Clostridioides Difficile Infection Through Dilution/Extinction, Jennifer M. Auchtung, Eva C. Preisner, James Collins, Armando I. Lerma, Robert A. Britton

Department of Food Science and Technology: Faculty Publications

The gastrointestinal microbiome plays an important role in limiting susceptibility to infection with Clostridioides difficile. To better understand the ecology of bacteria important for C. difficile colonization resistance, we developed an experimental platform to simplify complex communities of fecal bacteria through dilution and rapidly screen for their ability to resist C. difficile colonization after challenge, as measured by 􏰀100-fold reduction in levels of C. difficile in challenged communities. We screened 76 simplified communities diluted from cultures of six fecal donors and identified 24 simplified communities that inhibited C. difficile colonization in vitro. Sequencing revealed that simplified communities were composed of …

Microbiota In Vitro Modulated With Polyphenols Shows Decreased Colonization Resistance Against Clostridioides Difficile But Can Neutralize Cytotoxicity, Aleksander Mahnic, Jennifer M. Auchtung, Nataša Poklar Ulrih, Robert A. Britton, Maja Rupnik

Department of Food Science and Technology: Faculty Publications

While the knowledge on gut microbiota - C. difficile interactions has improved over the years, the understanding of the underlying mechanisms providing colonization resistance as well as preventative measures against the infection remain incomplete. in this study the antibiotic clindamycin and polyphenol extracts from pomegranate and blueberries were used individually and in combination to modulate fecal microbial communities in minibioreactor arrays (MBRA). Modulated communities were inoculated with C. difficile (ribotype 027). Subsequent 7-day periodical monitoring included evaluation of C. difficile growth and activity of toxins tcdA and tcdB as well as analysis of MBRA bacterial community structure (V3V4 16 S …

Phytochemical Characterization Of Ultrasound-Processed Sorghum Sprouts For The Use In Functional Foods, Sadia Hassan, Muhammad Imran, Muhammad Haseeb Ahmad, Muhammad Imran Khan, Changmou Xu, Muhammad Kamran Khan, Niaz Muhammad

Department of Food Science and Technology: Faculty Publications

In the recent years, application of ultrasound has been taken into considera- tion to enhance the nutritive value of processed food products, either by retaining or modulating their phytochemical components. Profile of phyto- chemicals in sorghum seeds was amended by optimizing ultrasound appli- cation for their use as sprouts. In this study, overall impact on various phytochemical constituents (alkaloids, phytates, saponins, and sterols), radi- cal scavenging activity (2,2-diphenyl-1-picrylhydrazyl assay, ferric reducing antioxidant power assay, and oxygen radical absorbance capacity assay assay), phenolic profile (total phenolics content, total flavonoids content, ferulic acid, gallic acid, catechin, quercetin, and tannin) along with in …

Fecal Short-Chain Fatty Acid Concentrations Increase In Newly Paired Male Marmosets (Callithrix Jacchus), Lifeng Zhu, Mallory J. Suhr Van Haute, Haley R. Hassenstab, Caroline Smith, Devin J. Rose, Aaryn C. Must, Andrew K. Benson, Jeffrey A. French

Department of Food Science and Technology: Faculty Publications

The role by which the gut microbiome influences host health (e.g., energy equilibrium and immune system) may be partly mediated by short-chain fatty acids, which are bacterial fermentation products from the dietary fibers. However, little is known about longitudinal changes in gut microbiome metabolites during cohabitation alongside social contact. In common marmosets (Callithrix jacchus), the gut microbiome community is influenced by social contact, as newly paired males and females develop convergent microbial profiles. Here, we monitored the dynamics of short-chain fatty acid concentrations in common marmoset feces from the prepairing (PRE) to postpairing (POST) stages. In males, we observed that …

Wild Primate Microbiomes Prevent Weight Gain In Germ-Free Mice, Dimitrios N. Sidiropoulos, Gabriel A. Al-Ghalith, Robin R. Shields-Cutler, Tonya L. Ward, Abigail J. Johnson, Pajau Vangay, Dan Knights, Purna C. Kashyap, Yibo Xian, Amanda E. Ramer-Tait, Jonathan B. Clayton

Department of Food Science and Technology: Faculty Publications

Background: The gut microbiome harbors trillions of bacteria that play a major role in dietary nutrient extraction and host metabolism. Metabolic diseases such as obesity and diabetes are associated with shifts in microbiome composition and have been on the rise in Westernized or highly industrialized countries. At the same time, Westernized diets low in dietary fiber have been shown to cause loss of gut microbial diversity. However, the link between microbiome composition, loss of dietary fiber, and obesity has not been well defined.

Results: To study the interactions between gut microbiota, dietary fiber, and weight gain, we transplanted captive and …

Stool Microbiome, Ph And Short/Branched Chain Fatty Acids In Infants Receiving Extensively Hydrolyzed Formula, Amino Acid Formula, Or Human Milk Through Two Months Of Age, Car Reen Kok, Bradford Brabec, Maciej Chichlowski, Cheryl L. Harris, Nancy Moore, Jennifer L. Wampler, Jon Vanderhoof, Devin Rose, Robert W. Hutkins

Department of Food Science and Technology: Faculty Publications

Background: Early infant feeding with intact or extensively hydrolyzed (EH) proteins or free amino acids (AA) may differentially affect intestinal microbiota composition and immune reactivity. This multicenter, double-blind, controlled, parallel-group, pilot study compared stool microbiota from Baseline (1–7 days of age) up to 60 days of age in healthy term infants who received mother’s own milk (assigned to human milk [HM] reference group) (n = 25) or were randomized to receive one of two infant formulas: AA-based (AAF; n = 25) or EH cow’s milk protein (EHF; n = 28). Stool samples were collected (Baseline, Day 30, Day 60) and …

Point-Of-Decision Prompts Increase Dietary Fiber Content Of Consumers’ Food Choices In An Online Grocery Shopping Simulation, Kristina Arslain, Christopher Gustafson, Devin J. Rose

Department of Food Science and Technology: Faculty Publications

Abstract: Only 5% of Americans consume the recommended amount of dietary fiber. In an online simulated shopping experiment, we examined whether a fiber-focused point-of-decision prompt (PDP) would influence consumers to choose food products that were higher in this important nutrient. We hypothesized that participants exposed to the dietary fiber PDP would choose products with more dietary fiber/serving than those who were not exposed to the PDP. The experiment was completed by 753 participants. Participants were randomly assigned to a condition in which they were not exposed to a PDP (the no-PDP condition), a personalized PDP, or PDP without personalization. Choices …

The International Scientific Association For Probiotics And Prebiotics (Isapp) Consensus Statement On The Definition And Scope Of Synbiotics, Kelly S. Swanson, Glenn R. Gibson, Robert W. Hutkins, Raylene A. Reimer, Gregor Reid, Kristin Verbeke, Karen P. Scott, Hannah D. Holscher, Meghan B. Azad, Nathalie M. Delzenne, Mary Ellen Sanders

Department of Food Science and Technology: Faculty Publications

In May 2019, the International Scientific Association for Probiotics and Prebiotics (ISAPP) convened a panel of nutritionists, physiologists and microbiologists to review the definition and scope of synbiotics. The panel updated the definition of a synbiotic to “a mixture comprising live microorganisms and substrate(s) selectively utilized by host microorganisms that confers a health benefit on the host”. The panel concluded that defining synbiotics as simply a mixture of probiotics and prebiotics could suppress the innovation of synbiotics that are designed to function cooperatively. Requiring that each component must meet the evidence and dose requirements for probiotics and prebiotics individually could …

Stearidonic-Enriched Soybean Oil Modulates Obesity, Glucose Metabolism, And Fatty Acid Profiles Independently Of Akkermansia Muciniphila, Rafael R. Segura Munoz, Truyen Quach, João C. Gomes-Neto, Yibo Xian, Pamela A. Pena, Steven Weier, Michael A. Pellizzon, Hatem Kittana, Liz A. Cody, Abby L. Geis, Kari Heck, Robert J. Schmaltz, Laure B. Bindels, Edgar B. Cahoon, Andrew K. Benson, Tom Elmo Clemente, Amanda E. Ramer-Tait

Department of Food Science and Technology: Faculty Publications

Scope: Previous studies have suggested that diets rich in omega-3 and low in omega-6 long- longchain polyunsaturated fatty acids (PUFAs) can limit the development of metabolic syndrome (MetS). Transgenic soybeans yielding oils enriched for omega-3 PUFAs represent a new and readily-available option for incorporating omega-3 PUFAs into diets to provide health benefits. Methods and Results: Transgenic soybean oils, enriched for either stearidonic acid (SDA) or eicosapentaenoic acid (EPA), are incorporated into diets to test their effects on limiting the development of MetS in a mouse model of diet-induced obesity. Supplementation with SDA- but not EPA-enriched oils improved features …

Polyphenolic Fractions Isolated From Red Raspberry Whole Fruit, Pulp, And Seed Differentially Alter The Gut Microbiota Of Mice With Diet-Induced Obesity, Yibo Xian, Rong Fan, Jing Shao, Ashley Mulcahy Toney, Soonkyu Chung, Amanda E. Ramer-Tait

Department of Food Science and Technology: Faculty Publications

Polyphenol extracts from red raspberry (RR) whole fruit or pulp, but not seed, attenuate high-fat (HF) dietinduced obesity in mice. Because host metabolism is linked to the microbiota, we investigated the effects of polyphenols from RR fruit, pulp, and seed on the microbiome. RR polyphenols significantly decreased the abundance of specific taxa that were increased during HF feeding relative to a low-fat diet, including Ruminococcus and an unclassified genus from Clostridiales. Compared to a HF diet, pulp and seed polyphenols increased Roseburia abundance and decreased levels of an unclassified genus from Mogibacteriaceae. RR seed polyphenols uniquely increased Bifidobacterium compared …

A Mouse Model Of Human Tlr4 D299g/T399i Snps Reveals Mechanisms Of Altered Lps And Pathogen Responses, Katharina Richard, Kurt H. Piepenbrink, Kari Ann Shirey, Archana Gopalakrishnan, Shreeram Nallar, Daniel J. Prantner, Darren J. Perkins, Wendy Lai, Alexandra Vik, Vladimir Y. Toshchakov, Chiguang Feng, Rachel Fanaroff, Andrei E. Medvedev, Jorge C.G. Blanco, Stefanie N. Vogel

Department of Food Science and Technology: Faculty Publications

Two cosegregating single-nucleotide polymorphisms (SNPs) in human TLR4, an A896G transition at SNP rs4986790 (D299G) and a C1196T transition at SNP rs4986791 (T399I), have been associated with LPS hyporesponsiveness and differential susceptibility to many infectious or inflammatory diseases. However, many studies failed to confirm these associations, and transfection experiments resulted in conflicting conclusions about the impact of these SNPs on TLR4 signaling. Using advanced protein modeling from crystallographic data of human and murine TLR4, we identified homologous substitutions of these SNPs in murine Tlr4, engineered a knock-in strain expressing the D298G and N397I TLR4 SNPs homozygously, and characterized in vivo …

Production And Properties Of Resistant Maltodextrin From Cassava Starch, Kamonrat Trithavisup

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aimed to determine the effects of processing conditions on molecular structure and selected properties of cassava-derived resistant dextrins (RDs) and resistant maltodextrins (RMDs). Cassava starch was mixed with hydrochloric acid solution to obtain the final acid concentration of 0.04-0.10% dry starch basis, dried at 50oC until having <5% moisture, and dextrinized at 100-120oC for 60-180 min. As acid concentration, heating temperature and time increased, water solubility, reducing sugar content, total dietary fiber (TDF) content and proportion of high molecular weight fiber fraction of RDs increased while their whiteness decreased. For the solution containing RDs produced under mild conditions (0.04-0.08% HCl, 100°C, 60 min), an endothermic peak at 45-70oC, having enthalpy of 1.66-2.14 J/g was detected. However, no endotherm was detected from the solution of RDs processed under extreme conditions. Harsher dextrinization conditions resulted in the RDs with slightly higher molecular weight but containing shorter branched chains. Dextrinization resulted in the formation of α-1,2, α-1,6, β-1,2, β-1,4 and β-1,6 in RDs. Lower proportion of α-1,4 linkage and α-/β-reducing ends with higher proportion of β-1,2, β-1,4, α-/β-1,6 linkages and degree of branching (DB) were found in RDs prepared under stronger dextrinization conditions. Although indigestible portion was enhanced by dextrinization, TDF of the RDs were only 7-47%. To increase the TDF content, RDs prepared from 5 different conditions (0.04% and 0.06% HCl, 120oC, 60-180 min), having >30% TDF, were selected for RMD production. The 30% RD solutions were treated with commercial α-amylase enzyme at 70oC, pH 6, for 90 and 120 min, to obtain the final dextrose equivalent of 8 and 12, respectively. The samples were then purified and spray dried. Alpha-amylase treatment produced RMD with shorter …

How Fishy Is Your Fish? Authentication, Provenance And Traceability In Fish And Seafood By Means Of Vibrational Spectroscopy, Aoife Power, Daniel Cozzolino

Articles

Food authenticity, traceability and provenance are emerging issues of major concern forconsumers, industries and regulatory bodies worldwide. In addition, both food safety and securityare an intrinsic component of food quality where the above issues are key in modern traceabilityand management systems. It has been reported that substitution of a high-quality species by lessexpensive ones might be a frequent practice in seafood products such as fish and shellfish. In thistype of products, the source (e.g., origin) and identification of the species are complex. Althoughdifferent countries have implemented strict regulations and labelling protocols, these issues still are ofconcern. This article briefly reviews …

Food Accessibility Related To The Double Your Dollar Program, Julia Carlson, Heather Friedrich, Mechelle Bailey, Curt Rom

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The Double Your Dollar (DYD) Program is a program that gives Supplemental Nutrition Assistance Program (SNAP) beneficiaries and Senior Farmers Market Nutrition Program (SFMNP) participants match dollars to spend at local farmers markets. The DYD’s goal is to incentivize healthy eating among individuals of low income and promote spending at farmers markets. Food insecurity affects over 60,000 individuals in Washington and Benton counties in Arkansas. The aim of this study was to assess how the DYD program impacted users’ food accessibility and how the program could be improved for the future. A survey was used to address basic demographics, type, …

Comparative Study On Chinese Network Imported Food Safety Supervision System, Jingquan Wang

Theses

This paper aims to improve the efficiency of food safety supervision and the effective way to ensure food safety through the comparative study of the imported food safety supervision system of China's network, and the comparative study of the main methods to ensure food safety. In this paper, according to the basic theory of food safety, for the world's major developed countries government food safety regulatory system, the Chinese government's food safety regulatory system research, China's food regulatory measures related to a new model idea, several parts, and discover network imported food safety supervision system in our country's own insufficiency, …

Vitamin D Fortification Of Processed Meat To Reduce Self-Reported Deficiencies: Consumer Acceptance In The Irish Market, Emma Lynn Donaldson

Theses

It is believed that many areas of the world are experiencing a Vitamin D deficiency pandemic. With Ireland’s geographic location, genetic characteristics, and the reemergence of rickets in Ireland in the early noughties; Ireland is a part of the deficiency pandemic. Vitamin deficiencies have historically been handled effectively through food fortification schemes, however additional food products, like processed meats, need to be assessed for their ability to address the deficiency and consumer acceptance. Aim: The aim of this study is to analyse the general consumer acceptance of future Vitamin D fortified, processed meats in the Irish market, in order to …

The Efficacy Of Lactic Acid And Steam Vacuum Applications In Reducing Microbial Load And Prolonging The Shelf Life Of Beer Carcases, Rachel Smith

Theses

Many foodborne outbreaks occur globally each year compromising consumer confidence and highlighting the need for continued improvements with regards to product safety. Therefore, intervention decontamination applications are currently gaining a lot of attention within the beef industry as an additional measure to improve the microbiological condition of finished products. Many studies have shown decontamination methods to be effective, however variations within the published literature makes scientific comparison difficult. This aim of this study is to determine the efficacy of a 2-3% lactic acid solution and steam vacuum technologies as intervention methods in reducing the microbial load and extending the shelf …

Comparison Of Allergen Legislation Between India And Ireland, Naresh Machha

Theses

2 Abstract Food allergen is one of the major issues in countries like Ireland and India. In Ireland, allergen and ingredients labelling are considered as the topmost problems in the Irish food business. The problems can be solved under a strict framework guided by strong regulations. The problem is bigger in India than Ireland as in India, there is no proper legalized standard in the context of food allergens so the people who are allergic to certain food products are on high risk. Labelling is a major issue in India due to pluralism and diverse linguistic barriers. Ireland operated FSAI …

An Investigation Into The Prevalence Of Food Hypersensitivity Amongst Children In The Early Years Service In Ireland, Ruta Patel

Theses

In the early years services in Ireland, the study studied the prevalence of hypersensitivity to food and the associated food among children between the aging of 0 and 12. Participants were 980 EYS recorded with the State and Department of Education of the Republic of Ireland and Northern Ireland during 2019. In the descriptive-analytical report, the management guideline for answering these questions by the EYS was circulated electronically via emails and included information regarding class, age, food hypersensitivity and food that induce hypersensitivity among children. Results on the prevalence of allergens to Food and Coeliac diseases and current policies to …

Food Proteins: Sustainable Sources Of Proteins Responsible For Emulsions Stability In Food Products, Gleicy Kelly Oliveira Nogueria

Theses

One of the major challenges manufactures are facing is the fact that consumer’s personal health standards are changing constantly, modern consumers are willing to know more about the ingredients used in their food. The relationship with ethics and sustainability is leading to changes in the food industry. Seen this, manufactures are opting for other sources of ingredients, such as plant-based alternatives. However, the use of these sort of products need to be developed due to of their unknown properties. The use of sustainable plant proteins provides a variety of benefits to the product, such as added nutritional value as fiber, …