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Dreissena In The Great Lakes: Benthic Community Impacts And Facilitation, Sonya C. Bayba May 2020

Dreissena In The Great Lakes: Benthic Community Impacts And Facilitation, Sonya C. Bayba

Great Lakes Center Masters Theses

Non-native invasive Dreissena mussels have altered the flow of energy in numerous freshwater systems by diverting nutrients from pelagic to littoral benthic food-webs, thus reducing primary production available to deep offshore benthos. Though the effect of the nearshore-dwelling Dreissena polymorpha (zebra mussel) on benthos has been studied thoroughly, little is known about the deep, offshore impacts of Dreissena rostriformis bugensis (quagga mussel). In the last two decades, quagga mussels have displaced zebra mussels in shallow Great Lakes regions and colonized previously uninhabited offshore lake regions. It is likely that the spread of quagga mussels into deep profundal areas has resulted …


Effects Of Light, Nutrients, And Salts On Microbial Biofilm Productivity And Detrital Processing In Aquatic Mesocosms, Bethanie Brooke Howard-Parker May 2020

Effects Of Light, Nutrients, And Salts On Microbial Biofilm Productivity And Detrital Processing In Aquatic Mesocosms, Bethanie Brooke Howard-Parker

Graduate Theses and Dissertations

Anthropogenic activities associated with urbanization, agriculture, and resource extraction continue to increase to support increasing needs of the growing population. These activities increase the amounts of pollutants entering freshwater streams and put aquatic ecosystems at structural and functional risk. Aquatic microbes play an important role in detrital processing in streams as a key linkage in moving carbon from detrital stocks into aquatic food webs. My research investigates the effects of light, nutrients, and salts on detrital microbes and decomposition in freshwaters using a mesocosm approach. In chapter one, I modified a current priming effect (PE) hypothesis model to include light …


Chefs' Perceptions Of Zero Waste Cooking In Restaurants, Josephine Reardon May 2020

Chefs' Perceptions Of Zero Waste Cooking In Restaurants, Josephine Reardon

Graduate Theses and Dissertations

The purpose of this study is to examine chefs’ perceptions and practices relative to current and potentially future efforts to decrease food waste. Through qualitative inquiry, this research aims to identify food waste reduction practices in Northwest Arkansas restaurants; chef’s perceptions of these practices and zero waste cooking; the impact these practices have on successfully implementing sustainability; and the biggest challenges in reducing food waste in restaurants. The results of this study will assist chefs and restaurant owners by providing guidance on practices easily utilized in restaurants currently.

An interview protocol, with five sections, was conducted with ten chefs at …


Remediation Of Crude Petroleum Oil-Water Emulsions Using Microalgae, Mohamed Shafi May 2020

Remediation Of Crude Petroleum Oil-Water Emulsions Using Microalgae, Mohamed Shafi

Civil and Environmental Theses

Crude petroleum oil spills are among the most important organic contaminations, which result from uncontrolled releases and spillages during transportation or storage. The separated oils on that accumulate on top of the water can be removed by various conventional skimming methods. However, the emulsified portions that remain within the water phase are more difficult to remove and pose significant threats to the environment and could tamper the tertiary treatment in a wastewater treatment plant. Biological treatment, using bacteria, have proven to be an effective method in the removal of the emulsified oils. However, the biomass produced in this case does …


Soil Amendment And Soil Testing As Nutrient Reduction Strategies For The Peel Integrated Water Initiative, Robert Summers, Peta Richards, David Weaver, David Rowe May 2020

Soil Amendment And Soil Testing As Nutrient Reduction Strategies For The Peel Integrated Water Initiative, Robert Summers, Peta Richards, David Weaver, David Rowe

Resource management technical reports

The Transform Peel program focuses on 42 000 hectares (ha) due east of Mandurah called the Peel Food Zone, 75 kilometres (km) south of Perth in the shires of Murray and Serpentine Jarrahdale and includes an assessment of intensified and enclosed agriculture and a business park. The Peel Integrated Water Initiative was developed through Transform Peel to identify water sources and minimise the effect of these projects on water quality by reducing the nutrient loads discharged into the Peel–Harvey Estuarine System, which has been suffering from poor water quality for this reason.

The mineral sand miner MZI Resources Pty Ltd …


Detection And Enumeration Of Bacterial Pathogens In The American Oyster (Crassostrea Virginica), Mohammad Maruf Billah May 2020

Detection And Enumeration Of Bacterial Pathogens In The American Oyster (Crassostrea Virginica), Mohammad Maruf Billah

Theses and Dissertations

American oyster (Crassostrea virginica) is a popular seafood for its delicacy and high nutritional value. Based on increasing concern about contamination of bacterial pathogens in raw oyster, my research objectives have been focused on detection and enumeration of two important bacterial pathogens, Escherichia coli and Salmonella spp. in the American oyster in south Texas waters, local markets and controlled laboratory studies. Immunohistochemical and RT-PCR analyses showed substantial bacterial pathogen’s presence in gills and digestive glands of oysters collected from San Martin Lake and South Padre Island as well as local markets. Laboratory studies showed increasing trend of both …


A Tale Of Two Species: Black-Tailed And White-Tailed Prairie Dog Biogeography From The Last Interglacial To 2070, April Dawn Bledsoe May 2020

A Tale Of Two Species: Black-Tailed And White-Tailed Prairie Dog Biogeography From The Last Interglacial To 2070, April Dawn Bledsoe

Electronic Theses and Dissertations

Ecological niche models (ENMs) were created for White-tailed and Black-tailed prairie dogs and projected into the Last Interglacial (LI), the Last Glacial Maximum (LGM), and mid-Holocene (mid-H) to discern possible past suitable habitat for both species. Additionally, ENMs were projected into the future year 2070 representative concentration pathways (RCPs) 2.6 and 8.5 to discern how climate change may affect future habitat suitability. Kernel density estimations, minimum convex polygons, and median distribution centers of White-tailed and Black-tailed occurrence records were examined between time-periods to discern the effects of anthropogenic westward expansion on both species’ distributions. Current ENMs were constructed from commonly …


Quantifying Greenhouse Gas Emissions And Ammonia Volatilization From Soil Amended With Excreta From Ruminants Fed Alternative Forage-Based Diets, Samuel Park May 2020

Quantifying Greenhouse Gas Emissions And Ammonia Volatilization From Soil Amended With Excreta From Ruminants Fed Alternative Forage-Based Diets, Samuel Park

Graduate Theses and Dissertations

Forage-based ruminant diets supplemented with condensed tannins or polyphenolic-containing legumes may alter nitrogen (N) metabolism in the animal and reduce gas emissions from soil receiving excreta. The objective of the study was to determine if soil receiving urine or manure from sheep fed forage diets supplemented with condensed tannin or polyphenolic-containing legumes would decrease N gas emissions. Two field trials were conducted: in the first trial, sheep were fed alfalfa (Medicago sativa L.) silage diets supplemented with 0, 9, 18, or 27% sericea lespedeza (Lespedeza cuneate [Dumont de Courset] G. Don) (n = 4). In the second trial sheep were …


Characterization Of Microbial Communities Across Disease States And Environmental Conditions In Kemp’S Ridley (Lepidochelys Kempii) And Green Sea Turtles (Chelonia Mydas), Kerry L. Mcnally May 2020

Characterization Of Microbial Communities Across Disease States And Environmental Conditions In Kemp’S Ridley (Lepidochelys Kempii) And Green Sea Turtles (Chelonia Mydas), Kerry L. Mcnally

Graduate Doctoral Dissertations

All species of sea turtles are threatened or endangered, with various diseases and conditions affecting populations around the world. Understanding healthy populations as well as populations beset by disease conditions, such as fibropapillomatosis and cold-stunning, could lead to helpful tools in the conservation management and medical treatment needed to protect these species. Microbial communities, or the microbiome, at different body sites of sea turtles likely play important roles in the health of these animals, from aiding in digestion to immune system regulation. Disruption of these communities, either through disease and/or environmental factors, may play a role in disease processes and …


The Effects Of Temperature On Evacuation Rates And Absorption Efficiency Of Flathead Catfish, Zach Horstman Apr 2020

The Effects Of Temperature On Evacuation Rates And Absorption Efficiency Of Flathead Catfish, Zach Horstman

School of Natural Resources: Dissertations, Theses, and Student Research

Knowledge of fish gastric evacuation rates are a necessary component for both field and laboratory studies when trying to understand feeding rates, modeling energy budgets, and understanding trophic dynamics of aquatic ecosystems. Many freshwater fish encounter a broad range of environmental temperatures across life history stages, but the mechanistic link between temperature and physiological processes often remains poorly understood. We designed three recirculating aquatic systems capable of rearing Flathead Catfish Plyodictis olivaris in order to quantify gastric evacuation rates and gross energy absorption at three temperatures (17º C, 22º C and 25º C). We examined the relationship between temperature and …


Evaluation Of Metal Contamination And Soil Properties At Former Mine Sites In Poland, Kaitlyn Mcclung, Emily Brooks Apr 2020

Evaluation Of Metal Contamination And Soil Properties At Former Mine Sites In Poland, Kaitlyn Mcclung, Emily Brooks

Research and Creativity Symposium

Surface mining dramatically affects the environment, both where the resource extraction takes place and in the areas where mining wastes and overburden are deposited. Poland is Europe’s top coal producing country and is also home to many mines for pyrite, sand, and other mineral resources. We studied soil samples from three former mining areas in Poland: the Bełchatów and Smolnica coal mines, and the Piaseczno sulfur mine. Various substrates, amendments, and trees had been used for remediation, with activities taking place up to 36 years previously. The success of reforestation and soil development varied greatly, especially in pH, organic matter, …


How Can We Change Gut Microbiota For Healthy Lives?, Elizaveta Shmakova Apr 2020

How Can We Change Gut Microbiota For Healthy Lives?, Elizaveta Shmakova

Scientific Communication News

No abstract provided.


Fighting Waste And Feeding People: Exploring The Context Of Campus Food Waste And Student Recovery Efforts, Sarah Becker, Max Stout, Maddie Kuklentz, Savona Cerra Apr 2020

Fighting Waste And Feeding People: Exploring The Context Of Campus Food Waste And Student Recovery Efforts, Sarah Becker, Max Stout, Maddie Kuklentz, Savona Cerra

Environment and Sustainability Presentations

Wismer on Wheels is dedicated to our mission of not only reducing food waste on campus, but also reducing food insecurity in our wider community. The work of our team of over 35 student volunteers reflects that dedication. This past year we became the official Food Recovery Network chapter for Ursinus, broadened outreach efforts on- and off-campus, and boosted food waste education, all while recovering unprecedented quantities of food. In this presentation, we celebrate the accomplishments of our dedicated volunteers while exploring deeper questions about food waste on the Ursinus campus. Why are we seeing such massive food recovery numbers …


Biogeography Of Biological Control: Spatial Variation In Agent-Host Interactions, Nathan Harms Apr 2020

Biogeography Of Biological Control: Spatial Variation In Agent-Host Interactions, Nathan Harms

LSU Doctoral Dissertations

Management of plant invasions using biological control has the potential to generate spatial patterns which reflect geographic or genetic variation in invader or control agents. Despite its rarity in practice, investigations into the biogeography of interacting species (i.e., plant invader and control agent) in the context of biological control can lend insights into species distribution-abundance patterns and provide predictions for spatial variation in control success. I explored spatial variability in biological control agent-plant interactions using two wetland weed study systems with large geographic distributions: flowering rush (Butomus umbellatus L.) and alligatorweed (Alternanthera philoxeroides (Mart.) Griseb). Through literature and …


Environmental Activism, Ellen Dexter, Lydia Dexter Apr 2020

Environmental Activism, Ellen Dexter, Lydia Dexter

Honors Program: Expanded Learning Clubs

An after school book club that follows Greta Thunberg's book of speeches No One is Too Small to Make a Difference , as well as uses hands-on activities to teach the importance of environmental activism.


The Coastal Monitor Winter/Spring 2020, John Tanacredi Ph.D. Apr 2020

The Coastal Monitor Winter/Spring 2020, John Tanacredi Ph.D.

The Coastal Monitor

Throughout Long Island’s educational structure, STEM educational institutions have responded to the latest information from the Bureau of Labor Statistics who into our new year, have emphasized the fastest growing occupational categories over the next 10 years. These careers will be in the energy, health and education sectors, with the medical and technical sectors containing the highest paying occupations. Yet, hidden in the projected demand which will continue to spur on millions of new jobs, is corporate leaderships continuous claim that they cannot find qualified workers. This “skills gap” is due to a lack of strong cognitive and socio-economic skills. …


Water Current, Volume 52, No. 1, Spring 2020 Apr 2020

Water Current, Volume 52, No. 1, Spring 2020

Water Current Newsletter

MOD$$AT: Ogallala Water CAP’s Integrated Model

The Lowdown on Soil Moisture Monitoring

Innovations in Forages and Grazing in the High Plains


Temperature-Dependent Development And Survival Of Immature Stages Of The Coffee Berry Borer Hypothenemus Hampei (Coleoptera: Curculionidae), A. G.A. Azrag, A. A. Yusuf, C. W.W. Pirk, S. Niassy, K. K. Mbugua, R. Babin Apr 2020

Temperature-Dependent Development And Survival Of Immature Stages Of The Coffee Berry Borer Hypothenemus Hampei (Coleoptera: Curculionidae), A. G.A. Azrag, A. A. Yusuf, C. W.W. Pirk, S. Niassy, K. K. Mbugua, R. Babin

All Peer-Reviewed Publications

Although the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) is the most destructive insect pest of coffee worldwide, there is much to learn about its thermal biology. This study aimed to develop temperature-based models for H. hampei development and to provide the thermal requirements of immature stages in the laboratory. Using a new observation method, larval development and survival were monitored daily on fresh Arabica coffee seeds, under seven constant temperatures in the range 15-35°C, with 80 ± 5% RH and 12:12 L:D photoperiod. Linear and non-linear functions were fitted to the development data plotted against temperature, using …


Comparative Physiology Of Honey Bees (Apis Mellifera L.) Exposed To Chlorantraniliprole, Jennifer Williams Apr 2020

Comparative Physiology Of Honey Bees (Apis Mellifera L.) Exposed To Chlorantraniliprole, Jennifer Williams

Department of Entomology: Dissertations, Theses, and Student Research

One outstanding threat to the honey bee, Apis mellifera L., is the unintended exposure of these pollinators to agricultural pesticides. Anthranilic diamides, such as chlorantraniliprole, work in target pests through activation of the ryanodine receptor causing rapid feeding cessation, lethargy, paralysis, and eventual death. Chlorantraniliprole exhibits low acute toxicity to honey bees but relatively little information exists regarding effects of chlorantraniliprole exposure beyond mortality. The acute toxicity and metabolic detoxification enzyme activities following oral exposure were determined in the honey bee and the model crop pest, the fall armyworm (Spodoptera frugiperda, J.E. Smith). Additionally, chlorantraniliprole effects on honey bee …


Maize Growth, Yield, Water Productivity And Evapotranspiration Response To Different Irrigation Methods And Amounts And Different Timing And Methods Of Nitrogen Applications, Ali T. Mohammed Apr 2020

Maize Growth, Yield, Water Productivity And Evapotranspiration Response To Different Irrigation Methods And Amounts And Different Timing And Methods Of Nitrogen Applications, Ali T. Mohammed

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Declining the quantity and quality of freshwater resources in many parts of the world, including Midwestern USA, especially in the light of rapidly growing world’s population and changing climate, imposes significant and, in some cases imminent, challenges for producers, policy- and decision-makers to produce more yield with less water and other inputs, particularly in water scarcity regions.

There is not comprehensive previous research has quantified and evaluated coupled impacts of irrigation rates and nitrogen timing management strategies and their interactions on maize (Zea mays L.) productivity and its various attributed efficiency index metrics under different irrigation methods under the …


Food Frontiers: An Academically Sponsored New Journal, Li-Shu Wang, Jesus Simal-Gandara, Ming Du, Baiyi Lu, José L. Quiles, Zhen-Yu Chen, Bernhard Hennig, Mingfu Wang, Hang Xiao, Jayashree Arcot, Tianli Yue, Baodong Zheng, Xiaobo Zou, Yoshinori Marunaka, Lianzhong Ai, Weibin Bai, Maurizio Battino, Francesca Giampieri, Milen I. Georgiev, Xiaojun Liao, Youling L. Xiong Mar 2020

Food Frontiers: An Academically Sponsored New Journal, Li-Shu Wang, Jesus Simal-Gandara, Ming Du, Baiyi Lu, José L. Quiles, Zhen-Yu Chen, Bernhard Hennig, Mingfu Wang, Hang Xiao, Jayashree Arcot, Tianli Yue, Baodong Zheng, Xiaobo Zou, Yoshinori Marunaka, Lianzhong Ai, Weibin Bai, Maurizio Battino, Francesca Giampieri, Milen I. Georgiev, Xiaojun Liao, Youling L. Xiong

Superfund Research Center Faculty Publications

No abstract provided.


Operational Large-Area Land-Cover Mapping: An Ethiopia Case Study, Trevor Caughlin Mar 2020

Operational Large-Area Land-Cover Mapping: An Ethiopia Case Study, Trevor Caughlin

Biology Faculty Publications and Presentations

Knowledge of land cover and land use nationally is a prerequisite of many studies on drivers of land change, impacts on climate, carbon storage and other ecosystem services, and allows for sufficient planning and management. Despite this, many regions globally do not have accurate and consistent coverage at the national scale. This is certainly true for Ethiopia. Large-area land-cover characterization (LALCC), at a national scale is thus an essential first step in many studies of land-cover change, and yet is itself problematic. Such LALCC based on remote-sensing image classification is associated with a spectrum of technical challenges such as data …


Healthful Nutrition As A Prevention And Intervention Paradigm To Decrease The Vulnerability To Environmental Toxicity Or Stressors And Associated Inflammatory Disease Risks, Bernhard Hennig, Pan Deng Mar 2020

Healthful Nutrition As A Prevention And Intervention Paradigm To Decrease The Vulnerability To Environmental Toxicity Or Stressors And Associated Inflammatory Disease Risks, Bernhard Hennig, Pan Deng

Superfund Research Center Faculty Publications

No abstract provided.


Valorization Of Carrot Processing Waste, Alexandra M. Duval Mar 2020

Valorization Of Carrot Processing Waste, Alexandra M. Duval

Master's Theses

Commercial carrot processors produce up to 175,000 tons of carrot waste annually. Carrot Mash (CM) is the term referring to the waste by-product of peeled baby carrot processing. Transportation of carrot processing waste is expensive due to its high- water content (approx. 83-95%). High in bioactive compounds (carotenoids) and dietary fibers, it is expected that its conversion into a value-added by-product is of interest to the carrot processing industry. Hemicellulose-rich plant materials have proven to be a source of oligosaccharides, which are known for their beneficial prebiotic activity. The objectives of this research were to: 1) determine the effect of …


Predicting The Spatial Suitability Distribution Of Moringa Oleifera Cultivation Using Analytical Hierarchical Process Modelling, T. Tshabalala, B. Ncube, H. P. Moyo, E. M. Abdel-Rahman, O. Mutanga, A. R. Ndhlala Mar 2020

Predicting The Spatial Suitability Distribution Of Moringa Oleifera Cultivation Using Analytical Hierarchical Process Modelling, T. Tshabalala, B. Ncube, H. P. Moyo, E. M. Abdel-Rahman, O. Mutanga, A. R. Ndhlala

All Peer-Reviewed Publications

Moringa oleifera Lam, often grows well under cultivation in the tropics and sub-tropics. It does well in sandy to clayey soils and tolerates low rainfall. The plant is well-known for its nutritional and medicinal properties, hence it is fast gaining popularity in South Africa and the rest of the world. The objective of this study was to predict the suitable areas for cultivating M. oleifera in South Africa using climate and edaphic variables that significantly affect its growth and development. We used an Analytical Hierarchical Process (AHP) and Geographic Information System (GIS) weight function to assign suitability weights to criteria …


Effect Of Low-Use-Rate Zinc Fertilization On Rice Growth And Grain Yield, Maxwell D. Coffin, Nathan A. Slaton Mar 2020

Effect Of Low-Use-Rate Zinc Fertilization On Rice Growth And Grain Yield, Maxwell D. Coffin, Nathan A. Slaton

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Low-use-rate Zn fertilization methods have been developed and marketed for rice (Oryza sativa L.) fertilization with limited research validating their efficacy. Our research objectives were to evaluate the effect of Zn-seed treatment rate combined with six Zn-fertilization methods on early season canopy coverage, tissue-Zn concentration at the mid-tillering stage, and rice grain yield. The field experiment was conducted on six silt loams and one clay. Rice seed was treated with 0 or 3.3 g Zn kg(-1) as ZnO and combined with no Zn, granular ZnSO4 applied at 11 kg Zn ha(-1) (GRAN), 1.68 kg Zn ha(-1) as MicroEssentials (MESZ), 1.1 …


Tackling Regional Climate Change Impacts And Food Security Issues: A Critical Analysis Across Asean, Pif, And Saarc, Md. Saidul Islam, Edson Kieu Mar 2020

Tackling Regional Climate Change Impacts And Food Security Issues: A Critical Analysis Across Asean, Pif, And Saarc, Md. Saidul Islam, Edson Kieu

Research Collection Lee Kong Chian School Of Business

Climate change and food security issues are multi-faceted and transcend across national boundaries. Therefore, this paper begins with the premise that regional organizations are optimally positioned to address climate change and food security issues while actively engaging global partners to slow down or reverse current trajectories. However, the potential of regional organizations to play a central role in mitigating these vital concerns has not been realized. In this paper, we focus on three regional organizations—the Association of Southeast Asian Nations (ASEAN), the Pacific Islands Forum (PIF), and the South Asian Association for Regional Cooperation (SAARC) and set out to investigate …


Isotope-Based Methods For Evaluating Fish Trophic Geographies, Julie L. Vecchio Feb 2020

Isotope-Based Methods For Evaluating Fish Trophic Geographies, Julie L. Vecchio

USF Tampa Graduate Theses and Dissertations

Data on the movement and diets of fish during a variety of life stages are important inputs to fisheries stock assessments and marine ecosystem models. Stable isotopes may provide previously inaccessible information on movement and diet of a variety of managed and forage fish species. Here I used several novel means of interpretation for stable isotope data to infer diets and movements of several important fisheries species over both short (weeks to months) and long (lifetime) timescales. To calculate a constant partitioning offset (CPO) between the δ15N of muscle and of liver tissue, I conducted a literature search …


Economic Estimates Of Invasive Wild Pig Damage To Crops In 12 Us States, Sophie Mckee, Aaron Anderson, Keith Carlisle, Stephanie A. Shwiff Feb 2020

Economic Estimates Of Invasive Wild Pig Damage To Crops In 12 Us States, Sophie Mckee, Aaron Anderson, Keith Carlisle, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

We report the results of a survey on invasive wild pig (Sus scrofa L.) damage and control in 12 US states (Alabama, Arkansas, California, Florida, Georgia, Louisiana, Mississippi, Missouri, North Carolina, Oklahoma, South Carolina, and Texas). The crops chosen for this study represent the “second-tier” in terms of economic importance after the six crops that were the subject of Anderson et al. (2016). The survey was distributed by the USDA National Agricultural Statistical Service (NASS) in the summer of 2019 to a sample of producers in each of the states (except California) of the following six crops: hay, pecans (Carya …


Effects Of Deepwater Horizon Oil On Feather Structure And Thermoregulation In Gulls: Does Rehabilitation Work?, Katherine Horak, Nicole L. Barrett, Jeremy W. Ellis, Emma M. Campbell, Nicholas G. Dannemiller, Susan A. Shriner Feb 2020

Effects Of Deepwater Horizon Oil On Feather Structure And Thermoregulation In Gulls: Does Rehabilitation Work?, Katherine Horak, Nicole L. Barrett, Jeremy W. Ellis, Emma M. Campbell, Nicholas G. Dannemiller, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

Impacts of large-scale oil spills on avian species are far-reaching.While media attention often focuses on lethal impacts, sub-lethal effects and the impacts of rehabilitation receive less attention. The objective of our study was to characterize effects of moderate external oiling and subsequent rehabilitation on feather structure and thermoregulation in gulls. We captured 30 wild ring-billed gulls (Larus delawarensis) and randomly assigned each individual to an experimental group: 1) controls, 2) rehabilitated birds (externally oiled, rehabilitated by washing), or 3) oiled birds (externally oiled, not rehabilitated). We externally oiled birds with weathered MC252 Deepwater Horizon oil (water for controls) …