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Estimating Potential Exposure From Abandoned Uranium Mine Sites Through Machine Learning Classification Of Animal Behavior: A Case Study In The Navajo Nation, Christopher Girlamo
Estimating Potential Exposure From Abandoned Uranium Mine Sites Through Machine Learning Classification Of Animal Behavior: A Case Study In The Navajo Nation, Christopher Girlamo
Geography ETDs
Waste from Abandoned Uranium Mines (AUMs) has negatively impacted Indigenous communities for generations. To answer these concerns, this research collaborated with Navajo Nation community members in Cove, AZ and Red Valley, AZ in order to better predict the potential AUM waste exposure their flocks face. A GIS-Multi-Criteria Decision Analysis (GIS-MCDA) model quantified potential exposure to AUM waste. Animal behavior was classified based on GPS and accelerometer data utilizing a Hidden Markov Model (HMM). The results of both the GIS-MCDA and animal behavior classification modeling was combined into a cumulative exposure potential value for each animal. This study found that livestock …
Estimation Of Respiratory Disease Burden Attributed To Particulate Matter From Biomass Burning In Northern Thailand Using 1-Km Resolution Maiac-Aod, Anuttara Hongthong, Kampol Nanthapong, Thongchai Kanabkaew
Estimation Of Respiratory Disease Burden Attributed To Particulate Matter From Biomass Burning In Northern Thailand Using 1-Km Resolution Maiac-Aod, Anuttara Hongthong, Kampol Nanthapong, Thongchai Kanabkaew
Applied Environmental Research
The upper northern Thailand suffers from air pollution due to open burning, which has been known for a long time. It was also found that different respiratory diseases were attributed to air pollution, especially particulate matter. This study estimated the health impacts attributed to PM10 between 2014 and 2016 using the burden of disease in terms of the disability adjusted life year (DALYs). The spatial correlation was evaluated based on applicable remote sensing data using the geographically weighted regression (GWR) model. The average measured PM10 concentrations for the summer and annual periods between 2014 and 2016 were 73 and 89 …
Groundwater Potential Mapping At Highly Populated Sub-Districts Of Thimphu District Using Fuzzy Analytic Hierarchy Process, Thongley Thongley, Tshering Choki, Kuenzang Choden
Groundwater Potential Mapping At Highly Populated Sub-Districts Of Thimphu District Using Fuzzy Analytic Hierarchy Process, Thongley Thongley, Tshering Choki, Kuenzang Choden
Applied Environmental Research
Groundwater is important when there is an insufficient spring water supply for daily needs. The population growth and expansion of the cities demand more water. Thimphu is the capital city of Bhutan and facing water shortage in recent times due to urban expansion and population growth. This study aims to find the potential area for groundwater using the Fuzzy Analytic Hierarchy Process. The factors used for this study are elevation, slope, curvature, topographic wetness index, drainage density, normalized difference vegetation index, distance from fault density, rainfall distribution map, soil texture map, and drainage density. The rating for the factors and …
Evaluation Of E-Waste Generation Through G-Pestle Analysis In The City Of Zamboanga, Philippines: Basis For Management Strategies, Mary Ann Beth L. Kong, Edwin R. De Los Reyes, Francis Dave C. Siacor
Evaluation Of E-Waste Generation Through G-Pestle Analysis In The City Of Zamboanga, Philippines: Basis For Management Strategies, Mary Ann Beth L. Kong, Edwin R. De Los Reyes, Francis Dave C. Siacor
Applied Environmental Research
Due to a growth in the production of electronic waste (e-waste), the Philippines, particularly Zamboanga City, a top-tier, highly urbanized city on the Zamboanga Peninsula, is coping with an e-waste problem. Understanding the outside forces influencing e-waste management is necessary for creating effective strategies. With the help of a G-PESTLE analysis (geographical, political, economic, social, technical, legal, and environmental) and recommendations for improvement, this study assessed the city's existing e-waste management situation. The study looks at possibilities and problems, then offers management tactics based on the results. The study employed a hybrid methodology. Using the G-PESTLE analysis framework, the external …
Assessing The Compliance Of A Coastal Municipality To The Ecological Solid Waste Management Act Of 2000 (Ra 9003), Michelle Del Rosario
Assessing The Compliance Of A Coastal Municipality To The Ecological Solid Waste Management Act Of 2000 (Ra 9003), Michelle Del Rosario
Applied Environmental Research
To bring about a significant change in the SWM situation in the Philippines, the Ecological Solid Waste Management Act of 2000 (RA 9003) was enacted to provide local government units with frameworks that would help them solve the looming garbage problem in the country. Despite the presence of the decree, managing wastes remains a problem in the country particularly in the CALABARZON. This study was conducted to assess the extent of compliance of a coastal municipality to RA 9003 in terms of institutional mechanism, comprehensive solid waste management, incentives, and financial solid waste management and compare the assessments of 72 …
Dual-Polarimetric Radar Applications For Investigating Severe Thunderstorms In Northern Thailand During The Pre-Monsoon Season, Nattapon Mahavik, Sarintip Tantanee, Fatah Masthawee
Dual-Polarimetric Radar Applications For Investigating Severe Thunderstorms In Northern Thailand During The Pre-Monsoon Season, Nattapon Mahavik, Sarintip Tantanee, Fatah Masthawee
Applied Environmental Research
Understanding the physical characteristics of severe pre-monsoon thunderstorms is crucial for mitigating the adverse impacts of natural disasters on people in tropical regions. On April 23, 2020, a severe storm affected the Chiang Khong District of the Chiang Rai Province in Northern Thailand, causing devastation to more than 500 houses with hail and strong winds. This study investigated the storm's physical characteristics using dual-polarimetric radar data from the Thai Meteo-rological Department (TMD). Using a combination of polarimetric radar variables, such as reflectivity (ZH), specific differential phase (KDP), differential reflectivity (ZDR), and copolar correlation coefficient (ρhv), fuzzy logic was utilized to …
การเปลี่ยนแปลงการใช้ประโยชน์ที่ดินที่มีผลต่อความเสี่ยงในการเกิดความแห้งแล้งของพื้นที่ลุ่มน้ำลำตะคอง, นนทพร คุขุนทด
การเปลี่ยนแปลงการใช้ประโยชน์ที่ดินที่มีผลต่อความเสี่ยงในการเกิดความแห้งแล้งของพื้นที่ลุ่มน้ำลำตะคอง, นนทพร คุขุนทด
Chulalongkorn University Theses and Dissertations (Chula ETD)
ความแห้งแล้งเป็นภัยทางธรรมชาติที่สร้างความเสียหายให้แก่หลายประเทศทั่วโลกรวมทั้งประเทศไทย ซึ่งพื้นที่ส่วนใหญ่ที่ได้รับผลกระทบจากความแห้งแล้งตั้งอยู่ในภาคตะวันออกเฉียงเหนือของประเทศไทย หนึ่งในพื้นที่ที่ประสบปัญหาความแห้งแล้งอย่างต่อเนื่อง คือ พื้นที่ลุ่มน้ำลำตะคอง จังหวัดนครราชสีมา การศึกษาวิจัยครั้งนี้มีวัตถุประสงค์เพื่อวิเคราะห์ปัจจัยที่ส่งผลต่อความเสี่ยงในการเกิดความแห้งแล้ง และประเมินพื้นที่เสี่ยงในการเกิดความแห้งแล้งของพื้นที่ลุ่มน้ำลำตะคองในปี พ.ศ. 2564 โดยการประยุกต์ใช้กระบวนการวิเคราะห์ตามลำดับชั้น (Analytic Hierarchy Process: AHP) ร่วมกับระบบสารสนเทศทางภูมิศาสตร์ (GIS) และวิเคราะห์การเปลี่ยนแปลงรูปแบบการใช้ประโยชน์ที่ดินของพื้นที่ลุ่มน้ำจากอดีตถึงปัจจุบัน โดยการศึกษาวิเคราะห์ภาพถ่ายดาวเทียม Landsat-5 และ Landsat-7 จาก 3 ช่วงเวลาปี พ.ศ. 2543 พ.ศ. 2554 และ พ.ศ. 2564 พร้อมทั้งคาดการแนวโน้มการใช้ประโยชน์ที่ดินของพื้นที่ลุ่มน้ำในปี พ.ศ. 2575 ด้วยแบบจำลอง CA-Markov และประเมินความเสี่ยงในการเกิดความแห้งแล้งของพื้นที่ลุ่มน้ำในปี พ.ศ. 2575 อันเป็นผลมาจากการเปลี่ยนแปลงรูปแบบการใช้ประโยชน์ที่ดิน ผลการศึกษา พบว่า ปัจจัยที่ส่งผลต่อความเสี่ยงในการเกิดความแห้งแล้งมากที่สุดสามลำดับแรก คือ ปริมาณน้ำฝน ความถี่วันฝนตก และการใช้ประโยชน์ที่ดิน ซึ่งพื้นที่ส่วนใหญ่ของลุ่มน้ำลำตะคองเป็นพื้นที่ที่มีความเสี่ยงในการเกิดความแห้งแล้งระดับสูง กระจายตัวอยู่ทุกส่วนของพื้นที่ลุ่มน้ำ และการเปลี่ยนแปลงการใช้ประโยชน์ที่ดินของพื้นที่ลุ่มน้ำลำตะคองระหว่างช่วงเวลา 21 ปี (พ.ศ. 2543 – 2564) สอดคล้องกับผลการคาดการณ์การใช้ประโยชน์ที่ดินปี พ.ศ. 2575 ชี้ให้เห็นว่า ส่วนใหญ่เป็นการเปลี่ยนแปลงจากพื้นที่เกษตรกรรมไปเป็นการใช้ประโยชน์ที่ดินรูปแบบอื่น ๆ โดยเฉพาะพื้นที่ชุมชนและสิ่งปลูกสร้าง ทำให้พื้นที่ชุมชนและสิ่งปลูกสร้างมีขนาดเพิ่มขึ้นอย่างต่อเนื่อง ส่งผลให้ภาพรวมของพื้นที่ลุ่มน้ำลำตะคองมีระดับความเสี่ยงในการเกิดความแห้งแล้งสูงขึ้น
The Future Of Meat Substitutes For Sustainable Food System: Insights From Chinese Consumer Preference And Role Of Agenda Setting, Wenxuan Guo
Chulalongkorn University Theses and Dissertations (Chula ETD)
The future of meat substitutes for China's sustainable food system was examined in this dissertation. Influence of media agenda-setting, consumer perception, preferences, and experts' opinions towards sustainable meat systems were investigated by text mining, online questionnaire survey, and stakeholder in-depth interviews. Integration of the agenda-setting concerning meat substitutes with the 2022 Chinese government dietary policy and the influence of articles in mainstream news is recommended. The research findings contribute to bridging the gap between policy formulation, agenda-setting, and public perception and suggest that media provide information consistently with the national food policy and provide education and knowledge correctly and clearly. …
Assessment Of Microplastics In The Surface Water Of Sitio Pulo, Navotas, Metro Manila, Mark Lester C. Galicia, Carl Luis P. Flestado, Jeff Ryan S. Magalong, Ronald Benz L. Rivera, Kim M. Villacorte, William Peter G. Sarmiento, Chester C. Deocaris
Assessment Of Microplastics In The Surface Water Of Sitio Pulo, Navotas, Metro Manila, Mark Lester C. Galicia, Carl Luis P. Flestado, Jeff Ryan S. Magalong, Ronald Benz L. Rivera, Kim M. Villacorte, William Peter G. Sarmiento, Chester C. Deocaris
Applied Environmental Research
Microplastics are synthetic polymer particles with a length of 5 mm or less with no well-defined lower boundary. These debris particles are known to affect marine and aquatic organisms which poses a threat to biodiversity and marine resources. In this study, the isolated microplastic from the coastal and lagoon surface waters of Sitio Pulo is described. Sitio Pulo is a barrier island mangrove sanctuary located at Brgy. Tanza I, Navotas City surrounded by Manila Bay. The samples collected last July 4 and 25, 2019, were isolated, profiled, and analyzed. A total of four hundred forty-nine (449) microplastic fragments were isolated …
Decline Of Seagrass (Posidonia Oceanica) Production Over Two Decades In The Face Of Warming Of The Eastern Mediterranean Sea, Victoria Litsi-Mizan, Pavlos T. Efthymiadis, Vasilis Gerakaris, Oscar Serrano, Manolis Tsapakis, Eugenia T. Apostolaki
Decline Of Seagrass (Posidonia Oceanica) Production Over Two Decades In The Face Of Warming Of The Eastern Mediterranean Sea, Victoria Litsi-Mizan, Pavlos T. Efthymiadis, Vasilis Gerakaris, Oscar Serrano, Manolis Tsapakis, Eugenia T. Apostolaki
Research outputs 2022 to 2026
* The response of Posidonia oceanica meadows to global warming of the Eastern Mediterranean Sea, where the increase in sea surface temperature (SST) is particularly severe, is poorly investigated. * Here, we reconstructed the long-term P. oceanica production in 60 meadows along the Greek Seas over two decades (1997–2018), using lepidochronology. We determined the effect of warming on production by reconstructing the annual and maximum (i.e. August) SST, considering the role of other production drivers related to water quality (i.e. Chla, suspended particulate matter, Secchi depth). * Grand mean (±SE) production across all sites and the study period was 48 …
Teaching Agroecology: Preparing Students For Navigating Uncharted Territory, Charles A. Francis, Steve Gliessman
Teaching Agroecology: Preparing Students For Navigating Uncharted Territory, Charles A. Francis, Steve Gliessman
Department of Agronomy and Horticulture: Faculty Publications
Agroecologists understand that farming and food systems are more complex than the aggregation of their components. This realization drives our choices of learning strategies and activities that will prepare students for complexity and uncertainty. Our quest for a just, sustainable, and nutritious food system adequate to equitably serve everyone on the planet today and into the future is an enormous challenge. An undertaking of this magnitude will be met only with major adjustments informed by thoughtful teaching and practicing problem solving skills through a new educational lens. The principles of agroecology help us focus this lens on the wicked problems …
Assessment Of Habitat‐Specific Competition For Oral Rabies Vaccine Baits Between Raccoons And Opossums, James L. Helton, Jacob E. Hill, David A. Bernasconi, Wesley C. Dixon, Richard B. Chipman, Amy Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.
Assessment Of Habitat‐Specific Competition For Oral Rabies Vaccine Baits Between Raccoons And Opossums, James L. Helton, Jacob E. Hill, David A. Bernasconi, Wesley C. Dixon, Richard B. Chipman, Amy Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.
United States Department of Agriculture Wildlife Services: Staff Publications
Throughout the eastern United States, the National Rabies Management Program (NRMP) distributes oral rabies vaccine (ORV) baits to manage rabies virus circulation in raccoon (Procyon lotor) populations. The consumption of vaccine baits by non‐target species including Virginia opossums (Didelphis virginiana) may reduce the effectiveness of ORV programs, but competition for baits remains poorly quantified in many areas of the southeastern United States. We distributed placebo ORV baits injected with a biomarker across 4 land cover types (bottomland hardwood, upland pine, riparian, isolated wetland) on the Savannah River Site in South Carolina, USA, 2017–2019. We then trapped …
Genomic Analyses Of Gray Fox Lineages Suggest Ancient Divergence And Secondary Contact In The Southern Great Plains, Elizabeth M. Kierepka, Sophie Preckler-Quisquater, Dawn M. Reding, Antoinette Piaggio, Seth P.D. Riley, Benjamin N. Sacks
Genomic Analyses Of Gray Fox Lineages Suggest Ancient Divergence And Secondary Contact In The Southern Great Plains, Elizabeth M. Kierepka, Sophie Preckler-Quisquater, Dawn M. Reding, Antoinette Piaggio, Seth P.D. Riley, Benjamin N. Sacks
United States Department of Agriculture Wildlife Services: Staff Publications
The gray fox (Urocyon cinereoargenteus) lineage diverged from all other extant canids at their most basal node and is restricted to the Americas. Previous mitochondrial analysis from coastal populations identified deeply divergent (up to 1 Mya) eastern and western lineages that predate most intraspecific splits in carnivores. We conducted genotyping by sequencing and mitochondrial analysis on gray foxes sampled across North America to determine geographic concordance between nuclear and mitochondrial contact zones and divergence times. We also estimated the admixture within the contact zone between eastern and western gray foxes based on nuclear DNA. Both datasets confirmed that …
Effects Of Corn Supplementation On Serum And Muscle Microrna Profiles In Horses, Clarissa Carver, Jason E. Bruemmer, Stephen Coleman, Gabriele Landolt, Tanja Hess
Effects Of Corn Supplementation On Serum And Muscle Microrna Profiles In Horses, Clarissa Carver, Jason E. Bruemmer, Stephen Coleman, Gabriele Landolt, Tanja Hess
United States Department of Agriculture Wildlife Services: Staff Publications
Laminitis associated with equine metabolic syndrome causes significant economic losses in the equine industry. Diets high in non-structural carbohydrates (NSC) have been linked to insulin resistance and laminitis in horses. Nutrigenomic studies analyzing the interaction of diets high in NSCs and gene expression regulating endogenous microRNAs (miRNA) are rare. This study's objectives were to determine whether miRNAs from dietary corn can be detected in equine serum and muscle and its impacts on endogenous miRNA. Twelve mares were blocked by age, body condition score, and weight and assigned to a control (mixed legume grass hay diet) and a mixed legume hay …
H7n9 Influenza A Virus Transmission In A Multispecies Barnyard Model, Angela M. Bosco-Lauth, Anna Rodriguez, Rachel M. Maison, Stephanie M. Porter, J. Jeffrey Root
H7n9 Influenza A Virus Transmission In A Multispecies Barnyard Model, Angela M. Bosco-Lauth, Anna Rodriguez, Rachel M. Maison, Stephanie M. Porter, J. Jeffrey Root
United States Department of Agriculture Wildlife Services: Staff Publications
Influenza A viruses are a diverse group of pathogens that have been responsible for millions of human and avian deaths throughout history. Here, we illustrate the transmission potential of H7N9 influenza A virus between Coturnix quail (Coturnix sp.), domestic ducks (Anas platyrhynchos domesticus), chickens (Gallus gallus domesticus), and house sparrows (Passer domesticus) co-housed in an artificial barnyard setting. In each of four replicates, individuals from a single species were infected with the virus. Quail shed virus orally and were a source of infection for both chickens and ducks. Infected chickens transmitted the virus to quail but not to ducks or …
Writing Statistical Methods For Ecologists, Amy J. Davis, Shannon Kay
Writing Statistical Methods For Ecologists, Amy J. Davis, Shannon Kay
United States Department of Agriculture Wildlife Services: Staff Publications
The Methods section is a key component of any ecology research publication containing detailed information on how the data were collected and analyzed. However, descriptions of which statistical methods were used and how they were applied can substantially vary and may not provide enough information for the analyses to be reproducible. Computational and statistical programming advances have allowed ecological researchers without a strong statistical or mathematical background to access and use increasingly complex statistical methods. Thus, statistical methods are written by and need to be accessible to researchers across a range of quantitative expertise. Poorly written Methods sections can incorrectly …
Demographic, Environmental And Physiological Predictors Of Gastrointestinal Parasites In Urban Raccoons, Liana F. Wait, Shylo R. Johnson, Kathleen M. Nelson, Richard B. Chipman, Frederick E. Pogmore, Andrew P. Dobson, Andrea L. Graham
Demographic, Environmental And Physiological Predictors Of Gastrointestinal Parasites In Urban Raccoons, Liana F. Wait, Shylo R. Johnson, Kathleen M. Nelson, Richard B. Chipman, Frederick E. Pogmore, Andrew P. Dobson, Andrea L. Graham
United States Department of Agriculture Wildlife Services: Staff Publications
Raccoons are host to diverse gastrointestinal parasites, but little is known about the ecology of these parasites in terms of their interactions with each other during coinfections, their interactions with host physiology and environmental factors, and their impact on raccoon health and survival. As a first step, we investigated the patterns of parasite infection and their demographic distribution in an urban-suburban population of raccoons trapped in the summers and autumns of 2018 and 2019. We collected faecal samples, demographic data, morphometric measurements, and blood smears, and used GPS data to classify trapping location by land cover type. Faecal floats were …
Dispersal Of Blackbird Flocks From Sunflower Fields: Efficacy Influenced By Flock And Field Size But Not Drone Platform, Conor C. Egan, Bradley Blackwell, Esteban Fernández‐Juricic, Page Klug
Dispersal Of Blackbird Flocks From Sunflower Fields: Efficacy Influenced By Flock And Field Size But Not Drone Platform, Conor C. Egan, Bradley Blackwell, Esteban Fernández‐Juricic, Page Klug
United States Department of Agriculture Wildlife Services: Staff Publications
Crop depredation by blackbirds (Icteridae) results in substantial economic losses to the United States sunflower industry, and a solution to effectively reduce damage remains elusive. We evaluated the utility of uncrewed aircraft systems (UAS), or drones, as hazing tools to deter foraging blackbirds from commercial sunflower (Helianthus annuus) fields in North Dakota, USA, between September and October 2017. We compared the efficacy of 3 drones: a fixed‐wing predator model mimicking the form of an aerial raptor, a fixed‐wing airplane of similar size, and a multirotor drone. Multirotor drones are relatively easy to fly and are a multifunctional tool for agricultural …
The Influence Of Income And Loss On Hunters' Attitudes Towards Wild Pigs And Their Management, Samantha Leivers, Keith M. Carlisle, Rachel L. Connally, Maureen G. Frank, John M. Tomeček
The Influence Of Income And Loss On Hunters' Attitudes Towards Wild Pigs And Their Management, Samantha Leivers, Keith M. Carlisle, Rachel L. Connally, Maureen G. Frank, John M. Tomeček
United States Department of Agriculture Wildlife Services: Staff Publications
Wild pigs (Sus scrofa) are one of the most pervasive invasive species in North America. Wild pigs pose a threat to crops, livestock, and the environment, but also provide recreational hunting opportunities. There are avenues for some stakeholder groups to generate income from wild pigs, however, stakeholders vary in attitudes towards wild pigs and their management. We investigated whether financial loss and income resulting from wild pigs influenced hunter stakeholder attitudes towards their management in Texas. We examined how land use influenced hunting landowner attitudes towards wild pigs. We analyzed 22,176 responses (8,707 landowners, 13,469 nonlandowners) fromTexas hunters …
Intracellular Diversity Of Wnv Within Circulating Avian Peripheral Blood Mononuclear Cells Reveals Host-Dependent Patterns Of Polyinfection, Dalit Talmi-Frank, Alex D. Byas, Reyes Murrieta, James Weger-Lucarelli, Claudia Rückert, Emily N. Gallichotte, Janna A. Yoshimoto, Chris Allen, Angela M. Bosco-Lauth, Barbara Graham, Todd A. Felix, Aaron C. Brault, Gregory D. Ebel
Intracellular Diversity Of Wnv Within Circulating Avian Peripheral Blood Mononuclear Cells Reveals Host-Dependent Patterns Of Polyinfection, Dalit Talmi-Frank, Alex D. Byas, Reyes Murrieta, James Weger-Lucarelli, Claudia Rückert, Emily N. Gallichotte, Janna A. Yoshimoto, Chris Allen, Angela M. Bosco-Lauth, Barbara Graham, Todd A. Felix, Aaron C. Brault, Gregory D. Ebel
United States Department of Agriculture Wildlife Services: Staff Publications
Arthropod-borne virus (arbovirus) populations exist as mutant swarms that are maintained between arthropods and vertebrates. West Nile virus (WNV) population dynamics are host-dependent. In American crows, purifying selection is weak and population diversity is high compared to American robins, which have 100- to 1000-fold lower viremia. WNV passed in robins leads to fitness gains, whereas that passed in crows does not. Therefore, we tested the hypothesis that high crow viremia allows for higher genetic diversity within individual avian peripheral blood mononuclear cells (PBMCs), reasoning that this could have produced the previously observed hostspecific differences in genetic diversity and fitness. Specifically, …
Determination Of Residue Levels Of Rodenticide In Rodent Livers Offered Novel Diphacinone Baits By Liquid Chromatography-Tandem Mass Spectrometry, David A. Goldade, Shane Siers, Steven C. Hess, Robert T. Sugihara, Craig A. Riekena
Determination Of Residue Levels Of Rodenticide In Rodent Livers Offered Novel Diphacinone Baits By Liquid Chromatography-Tandem Mass Spectrometry, David A. Goldade, Shane Siers, Steven C. Hess, Robert T. Sugihara, Craig A. Riekena
United States Department of Agriculture Wildlife Services: Staff Publications
A specific and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of the anticoagulant rodenticide diphacinone (DPN) in mouse and rat liver. Tissue samples were extracted with a mixture of water and acetonitrile containing ammonium hydroxide. The extracted sample was cleaned up with a combination of liquid-liquid partitioning and dispersive solid phase extraction. Chromatographic separation was achieved using a Waters X-Bridge BEH C-18 LC column (50 mm, 2.1 mm ID, 2.5 μm particle size) with detection on a triple quadrupole mass spectrometer in multiple reaction monitoring (MRM) mode. The monitored transition for DPN was m/z …
Controllable Factors Affecting Accuracy And Precision Of Human Identification Of Animals From Drone Imagery, Landon R. Jones, Jared A. Elmore, B. Santhana Krishnan, Sathishkumar Samiappan, Kristine O. Evans, Morgan B. Pfeiffer, Brad Blackwell, Raymond Iglay
Controllable Factors Affecting Accuracy And Precision Of Human Identification Of Animals From Drone Imagery, Landon R. Jones, Jared A. Elmore, B. Santhana Krishnan, Sathishkumar Samiappan, Kristine O. Evans, Morgan B. Pfeiffer, Brad Blackwell, Raymond Iglay
United States Department of Agriculture Wildlife Services: Staff Publications
Surveying animal populations using drones (unoccupied aircraft systems [UAS]) provides numerous advantages; however, few best practices exist to survey animal communities with drones. Among myriad factors that can affect human identification and counts of animals from drone images, we focused on three factors typically controlled in the study design or by the drone pilot: flight altitude, camera angle, and time of day. Identifying interactions and patterns among these three variables represents an important first step to determining best survey practices. We used a drone to survey known numbers of eight animal decoy species, representing a range of body sizes and …
Biodiversity Indices And Random Forests Reveal The Potential For Striped Skunk (Mephitis Mephitis) Fecal Microbial Communities To Function As A Biomarker For Oral Rabies Vaccination, Matthew W. Hopken, Darby Gilfillan, Amy Gilbert, Antoinette J. Piaggio, Mikaela Samsel Hilton, James Pierce, Bruce Kimball, Zaid Abdo
Biodiversity Indices And Random Forests Reveal The Potential For Striped Skunk (Mephitis Mephitis) Fecal Microbial Communities To Function As A Biomarker For Oral Rabies Vaccination, Matthew W. Hopken, Darby Gilfillan, Amy Gilbert, Antoinette J. Piaggio, Mikaela Samsel Hilton, James Pierce, Bruce Kimball, Zaid Abdo
United States Department of Agriculture Wildlife Services: Staff Publications
Wildlife disease surveillance and monitoring poses unique challenges when assessing rates of population vaccination, immunity, or infection prevalence. Non-invasively detected biomarkers can help reduce risk to both animal and field personnel during wildlife disease management activities. In this study, we investigated the utility of fecal microbiome data collected from captive striped skunks (Mephitis mephitis) in predicting rabies virus vaccination and infection status. We sequenced the hypervariable region 4 (V4) of the bacterial 16S gene and estimated alpha and beta diversity across timepoints in three groups of skunks: vaccination then rabies virus infection, sham vaccination then rabies virus infection, and rabies …
Factors Affecting The Recovery Of Mexican Wolves In The Southwest United States, Stewart W. Breck, Amy J. Davis, John Oakleaf, David L. Bergman, Jim Devos, J. Paul Greer, Kim M. Pepin
Factors Affecting The Recovery Of Mexican Wolves In The Southwest United States, Stewart W. Breck, Amy J. Davis, John Oakleaf, David L. Bergman, Jim Devos, J. Paul Greer, Kim M. Pepin
United States Department of Agriculture Wildlife Services: Staff Publications
- Recovering and maintaining large carnivore populations is a global conservation challenge that requires better knowledge of the factors affecting their populations, particularly in shared landscapes (i.e. non-protected areas where people occupy and or utilize the land).
- The Mexican wolf (Canis lupus baileyi) is an endangered wolf subspecies being recovered on shared landscapes in the Southwest United States and Mexico. We used data from the U.S. program to model population growth, evaluate the impact of management removal and illegal killing relative to other demographic factors, and test hypotheses about factors influencing rates of management removal and illegal killing.
- From …
Can Demographic Histories Explain Long-Term Isolation And Recent Pulses Of Asymmetric Gene Flow Between Highly Divergent Grey Fox Lineages?, Sophie Preckler-Quisquater, Elizabeth M. Kierepka, Dawn M. Reding, Antoinette J. Piaggio, Benjamin N. Sacks
Can Demographic Histories Explain Long-Term Isolation And Recent Pulses Of Asymmetric Gene Flow Between Highly Divergent Grey Fox Lineages?, Sophie Preckler-Quisquater, Elizabeth M. Kierepka, Dawn M. Reding, Antoinette J. Piaggio, Benjamin N. Sacks
United States Department of Agriculture Wildlife Services: Staff Publications
Secondary contact zones between deeply divergent, yet interfertile, lineages provide windows into the speciation process. North American grey foxes (Urocyon cinereoargenteus) are divided into western and eastern lineages that diverged approximately 1 million years ago. These ancient lineages currently hybridize in a relatively narrow zone of contact in the southern Great Plains, a pattern more commonly observed in smaller-bodied taxa, which suggests relatively recent contact after a long period of allopatry. Based on local ancestry inference with whole-genome sequencing (n = 43), we identified two distinct Holocene pulses of admixture. The older pulse (500–3500 YBP) reflected unidirectional …
Raccoons (Procyon Lotor) Show Higher Trypanosoma Cruzi Detection Rates Than Virginia Opossums (Didelphis Virginiana) In South Carolina, Usa, David A. Bernasconi, Madison L. Miller, Jacob E. Hill, Pooja Gupta, Richard Chipman, Amy Gilbert, Olin E. Rhodes Jr., Guha Dharmarajan
Raccoons (Procyon Lotor) Show Higher Trypanosoma Cruzi Detection Rates Than Virginia Opossums (Didelphis Virginiana) In South Carolina, Usa, David A. Bernasconi, Madison L. Miller, Jacob E. Hill, Pooja Gupta, Richard Chipman, Amy Gilbert, Olin E. Rhodes Jr., Guha Dharmarajan
United States Department of Agriculture Wildlife Services: Staff Publications
Chagas disease, a significant public health concern in the Americas, is caused by a protozoan parasite, Trypanosoma cruzi. The life cycle of T. cruzi involves kissing bugs (Triatoma spp.) functioning as vectors and mammalian species serving as hosts. Raccoons (Procyon lotor) and opossums (Didelphis virginiana) have been identified as important reservoir species in the life cycle of T. cruzi, but prevalence in both species in the southeastern United States is currently understudied. We quantified T. cruzi prevalence in these two key reservoir species across our study area in South Carolina, USA, and …
Rabies Virus Serosurvey Of The Small Indian Mongoose (Urva Auropunctata) Across Multiple Habitats In Puerto Rico, 2014–21, Are R. Berentsen, Mel J. Rivera-Rodriguez, Fabiola B. Torres-Toledo, Amy J. Davis, Richard Chipman, Amy Gilbert
Rabies Virus Serosurvey Of The Small Indian Mongoose (Urva Auropunctata) Across Multiple Habitats In Puerto Rico, 2014–21, Are R. Berentsen, Mel J. Rivera-Rodriguez, Fabiola B. Torres-Toledo, Amy J. Davis, Richard Chipman, Amy Gilbert
United States Department of Agriculture Wildlife Services: Staff Publications
The small Indian mongoose (Urva auropuncata) is a rabies reservoir in Puerto Rico and accounts for over 70% of reported animal rabies cases annually. The presence of rabies virus-neutralizing antibodies (RVNA) is often used as a tool to measure exposure to rabies virus in wildlife populations. We conducted a serosurvey of mongooses at 11 sites representing six habitat types across Puerto Rico. We collected a serum sample from 464 individual mongooses during 2014–2021. Overall, 80/464 (17.0%; 95% confidence interval, 14.1–20.9%; 55 male, 23 female, and two sexes not recorded) of individual mongooses sampled across all habitats were RVNA …
Development And Evaluation Of Prototype Toxicant-Delivery Bait Stations For The Control Of The Small Indian Mongoose, Carmen C. Antaky, Robert T. Sugihara, Israel L. Leinbach, Shane R. Siers, Emily W. Ruell, Steven C. Hess
Development And Evaluation Of Prototype Toxicant-Delivery Bait Stations For The Control Of The Small Indian Mongoose, Carmen C. Antaky, Robert T. Sugihara, Israel L. Leinbach, Shane R. Siers, Emily W. Ruell, Steven C. Hess
United States Department of Agriculture Wildlife Services: Staff Publications
We conducted research to develop a safe and effective toxic bait to control the small Indian mongoose (Urva auropunctata), an invasive vertebrate predator impacting the survival of native species in Hawai‘i (United States) and in other parts of the world. A preserved fish-based bait product was found to be highly palatable to mongooses in cage trials and subsequent formulations with diphacinone (0.005%) showed promise as an efficacious toxic bait for mongooses. This product is intended for future use to control mongooses in conservation and urban areas, and as a biosecurity tool at ports of entry to address accidental …
Genomic Regions Associated With Pseudorabies Virus Infection Status In Naturally Infected Feral Swine (Sus Scrofa), Courtney F. Bowden, Jennifer N. Kiser, Ryan S. Miller, Alexandra C. Buckley, Paola M. Boggiatto, Rachael M. Giglio, Vienna R. Brown, Dorian Garrick, Holly L. Neibergs, Antoinette J. Piaggio, Scott E. Speidel, Timothy J. Smyser
Genomic Regions Associated With Pseudorabies Virus Infection Status In Naturally Infected Feral Swine (Sus Scrofa), Courtney F. Bowden, Jennifer N. Kiser, Ryan S. Miller, Alexandra C. Buckley, Paola M. Boggiatto, Rachael M. Giglio, Vienna R. Brown, Dorian Garrick, Holly L. Neibergs, Antoinette J. Piaggio, Scott E. Speidel, Timothy J. Smyser
United States Department of Agriculture Wildlife Services: Staff Publications
Pseudorabies virus (PRV)—the causative agent of Aujeszky’s disease—was eliminated from commercial pig production herds in the United States (US) in 2004; however, PRV remains endemic among invasive feral swine (Sus scrofa). The circulation of PRV among abundant, widespread feral swine populations poses a sustained risk for disease spillover to production herds. Risk–based surveillance has been successfully implemented for PRV in feral swine populations in the US. However, understanding the role of host genetics in infection status may offer new insights into the epidemiology and disease dynamics of PRV that can be applied to management strategies. Genetic mechanisms underlying …
Estimating Northern Spotted Owl (Strix Occidentalis Caurina) Pair Detection Probabilities Based On Call-Back Surveys Associated With Long-Term Mark-Recapture Studies, 1993–2018, Katie M. Dugger, Alan B. Franklin, Damon B. Lesmeister, Raymond J. Davis, J. David Wiens, Gary C. White, James D. Nichols, James E. Hines, Charles B. Yackulic, Carl J. Schwarz, Steven A. Ackers, L. Steven Andrews, Larissa L. Bailey, Robin Bown, Jesse Burgher, Kenneth P. Burnham, Peter C. Carlson, Tara Chestnut, Mary M. Conner, Krista E. Dilione, Eric D. Forsman, Scott A. Gremel, Keith A. Hamm, Dale R. Herter, J. Mark Higley, Rob B. Horn, Julianna M. Jenkins, William L. Kendall, David W. Lapmphear, Christopher Mccafferty, Trent L. Mcdonald, Janice A. Reid, Jeremy T. Rockweit, David C. Simon, Stan G. Sovern, James K. Swingle, Heather Wise
Estimating Northern Spotted Owl (Strix Occidentalis Caurina) Pair Detection Probabilities Based On Call-Back Surveys Associated With Long-Term Mark-Recapture Studies, 1993–2018, Katie M. Dugger, Alan B. Franklin, Damon B. Lesmeister, Raymond J. Davis, J. David Wiens, Gary C. White, James D. Nichols, James E. Hines, Charles B. Yackulic, Carl J. Schwarz, Steven A. Ackers, L. Steven Andrews, Larissa L. Bailey, Robin Bown, Jesse Burgher, Kenneth P. Burnham, Peter C. Carlson, Tara Chestnut, Mary M. Conner, Krista E. Dilione, Eric D. Forsman, Scott A. Gremel, Keith A. Hamm, Dale R. Herter, J. Mark Higley, Rob B. Horn, Julianna M. Jenkins, William L. Kendall, David W. Lapmphear, Christopher Mccafferty, Trent L. Mcdonald, Janice A. Reid, Jeremy T. Rockweit, David C. Simon, Stan G. Sovern, James K. Swingle, Heather Wise
United States Department of Agriculture Wildlife Services: Staff Publications
The northern spotted owl (Strix occidentalis caurina; hereinafter NSO) was listed as “threatened” under the Endangered Species Act in 1990 and population declines have continued since that listing. Given the species’ protected status, any proposed activities on Federal lands that might impact NSO require consultation with U.S. Fish and Wildlife Service and part of that consultation often includes surveys to determine presence and occupancy status of the species in the proposed activity area. The objective of this report is to present study-area specific estimates of the probability of detection for NSO pairs from twelve 2-week seasonal survey periods …