Digital Commons Network^™

Exciton Delocalization And Scaffold Stability In Bridged Nucleotide-Substituted, Dna Duplex-Templated Cyanine Aggregates, Simon K. Roy, Olga A. Mass, Donald L. Kellis, Christopher K. Wilson, John A. Hall, Bernard Yurke, William B. Knowlton

Electrical and Computer Engineering Faculty Publications and Presentations

Molecular excitons play a foundational role in chromophore aggregates found in light-harvesting systems and offer potential applications in engineered excitonic systems. Controlled aggregation of chromophores to promote exciton delocalization has been achieved by covalently tethering chromophores to deoxyribonucleic acid (DNA) scaffolds. Although many studies have documented changes in the optical properties of chromophores upon aggregation using DNA scaffolds, more limited work has investigated how structural modifications of DNA via bridged nucleotides and chromophore covalent attachment impact scaffold stability as well as the configuration and optical behavior of attached aggregates. Here we investigated the impact of two types of bridged nucleotides, …

Analysis Of Multiple Degree-Of-Freedom Oscillatory Aquatic Propulsion, Seth Alan Brooks

Dissertations - ALL

Propulsive performance and flow field data were experimentally measured for a two degree-of-freedom fish platform. The fish platform was designed and constructed based on the yellowfin tuna (\textit{Thunnus albacares}) that is known to be both fast and efficient. This work extends the current understanding of oscillatory aquatic propulsion to a three-dimensional fish platform with full volume flow field data and propulsive performance. A parametric sweep of trailing-edge amplitude of the caudal fin ($A$), heave-to-pitch ratio ($h^*$), and phase offset between the two degrees-of-freedom ($\phi$) was used to explore a parameter space that encompasses and extends beyond the known biological domain. …

Algorithm Hardware Codesign For High Performance Neuromorphic Computing, Haowen Fang

Dissertations - ALL

Driven by the massive application of Internet of Things (IoT), embedded system and Cyber Physical System (CPS) etc., there is an increasing demand to apply machine intelligence on these power limited scenarios. Though deep learning has achieved impressive performance on various realistic and practical tasks such as anomaly detection, pattern recognition, machine vision etc., the ever-increasing computational complexity and model size of Deep Neural Networks (DNN) make it challenging to deploy them onto aforementioned scenarios where computation, memory and energy resource are all limited. Early studies show that biological systems' energy efficiency can be orders of magnitude higher than that …

Rational Design Of Supported Metal Catalysts For Carbonyl Reduction During Biomass Upgrading, Xin Gao

Dissertations - ALL

Ketone hydrogenation:A model system of carbonyl (ketone) hydrogenation, acetone hydrogenation over supported Pt catalysts in the gas-phase fixed bed reactor was investigated to understand both the mechanism and rate controlling steps in a fundamental level. The experiments and outcomes include the detailed macro- and micro-kinetic analysis of hydrogenation of acetone. The apparent reaction orders of hydrogen and acetone, apparent activation energies, and observed kinetic isotope effects over a large range of temperatures and concentrations (compound partial pressures) are reported in this document. Generally speaking, the ketone hydrogenation over metal surfaces in vapor phase comply with the mechanism that hydrogen atoms …

The Integration Of Art: A Multiple Case Study Of Science, Technology, Engineering, Art, And Math (Steam) Schools In Three Schools In Southern California, Reyna Esther Smith

Dissertations

Purpose: The purpose of this qualitative multi-case study was to describe and analyze how schools that implement a STEAM program include the arts in their integrated program. The study focused on 3 K-12 schools in Southern California that are located in the Antelope Valley high desert region of the state. This multi-case study analyzed an elementary school, a middle school, and a high school that are in different districts in the area.

Methodology: This multi-case study used qualitative data to analyze the research questions regarding art infusion in STEAM programs and teacher and leader perspectives. Drawing from interviews of teachers …

Nondestructive Detection Of Codling Moth Infestation In Apples Using Pixel-Based Nir Hyperspectral Imaging With Machine Learning And Feature Selection, Nader Ekramirad, Alfadhl Y. Khaled, Lauren E. Doyle, Julia R. Loeb, Kevin D. Donohue, Raul T. Villanueva, Akinbode A. Adedeji

Electrical and Computer Engineering Faculty Publications

Codling moth (CM) (Cydia pomonella L.), a devastating pest, creates a serious issue for apple production and marketing in apple-producing countries. Therefore, effective nondestructive early detection of external and internal defects in CM-infested apples could remarkably prevent postharvest losses and improve the quality of the final product. In this study, near-infrared (NIR) hyperspectral reflectance imaging in the wavelength range of 900–1700 nm was applied to detect CM infestation at the pixel level for three organic apple cultivars, namely Gala, Fuji and Granny Smith. An effective region of interest (ROI) acquisition procedure along with different machine learning and data processing …

Substrate Delivery Of Embedded Liposomes, Srivatsan Kidambi, Stephen L. Hayward

Department of Chemical and Biomolecular Engineering: Faculty Publications

This invention relates to compositions useful for localized and sustained release of therapeutic agents, and more particularly to functionalized liposomes embedded in a poly electrolyte multilayer. Methods of preparing the compositions, methods of treating diseases, devices, and pharmaceutical compositions comprising the compositions are also provided.

Trip Based Modeling Of Fuel Consumption In Modern Heavy-Duty Vehicles Using Artificial Intelligence, Sasanka Katreddi, Arvind Thiruvengadam

Faculty & Staff Scholarship

Heavy-duty trucks contribute approximately 20% of fuel consumption in the United States of America (USA). The fuel economy of heavy-duty vehicles (HDV) is affected by several real-world parameters like road parameters, driver behavior, weather conditions, and vehicle parameters, etc. Although modern vehicles comply with emissions regulations, potential malfunction of the engine, regular wear and tear, or other factors could affect vehicle performance. Predicting fuel consumption per trip based on dynamic on-road data can help the automotive industry to reduce the cost and time for on-road testing. Data modeling can easily help to diagnose the reason behind fuel consumption with a …

The Effect Of Ph On Organic Carbon Uptake And Biological Phosphorus Removal By Tetrasphaera Polyphosphate Accumulating Organisms, Derek Belka

Civil Engineering ETDs

Tetrasphaera polyphosphate accumulating organisms (PAOs) have been found in greater abundance and may remove more P in full-scale enhanced biological phosphorus removal (EBPR) water resource recovery facilities (WRRF) worldwide than the more extensively studied Accumulibacter PAO. Understanding the effect that environmental conditions, such as pH, have on Tetrasphaera metabolisms is critical to understanding EBPR WRRF process upsets, achieving consistent low effluent P concentrations, and formulating optimization strategies. The objective of this study was to determine pH effects over the range from 5.5 to 8.5 on anaerobic amino acid uptake, P release, and cation release in a Tetrasphaera-enriched culture from …

Convergence Properties Of Solutions Of A Length-Structured Density-Dependent Model For Fish, Geigh Zollicoffer

Rose-Hulman Undergraduate Mathematics Journal

We numerically study solutions to a length-structured matrix model for fish populations in which the probability that a fish grows into the next length class is a decreasing nonlinear function of the total biomass of the population. We make conjectures about the convergence properties of solutions to this equation, and give numerical simulations which support these conjectures. We also study the distribution of biomass in the different age classes as a function of the total biomass.

The Development Of A Novel Blood-Brain Barrier, Organ-On-A-Chip System And The Identification Of Erythrocytes As A Key Contributor To Age-Associated Neurological Decline, Payam Amiri

KGI Theses and Dissertations

Advancements in modern medicine have greatly extended human life expectancy. However, with the onset of age-associated conditions such as Alzheimer’s disease, autoimmune disorders, and certain cancers, healthy aging is becoming an increasingly pressing issue. Blood exchanges between young and old partners demonstrate old blood can cause detrimental effect to the young animal, notably on the brain tissue. However, the complexity of blood makes it difficult to identify what role the individual factors play in the aggregate observed effect. This dissertation seeks to explore the potential effects of erythrocytes on the brain as we age. Erythrocyte morphology and rigidity changes as …

Detecting Viral Particles In Vitro Using Photoacoustic Flow Cytometry, Anie-Pier Samson

Electronic Theses and Dissertations

The Covid-19 pandemic is a powerful example of just how damaging the rapid spread of an unknown virus can be. Viruses have the ability to spread rapidly amongst individuals if not treated and controlled. The first key step towards treatment is the timely and specific diagnosis of the virus causing the infection. An innovative method for the rapid detection of viral particles in solution consists of tagging the viral particles paired with using photoacoustic flow cytometry to irradiate the particles and get a signal. The high affinity of Streptavidin for Biotin can be used to bind Streptavidin-coated microspheres to biotinylated …

Real-Time Monitoring Of Cell Death Progress Using Capacitance Spectroscopy, Suyang Wu

Electronic Theses and Dissertations

Biologics, including the monoclonal antibody (mAb), has experienced rapid development in the last decade. However, the price of biologics is often prohibitively high because of the low process efficiency. Delaying the inevitable cell death improves the productivity of upstream bioprocessing, whose success relies on monitoring the cell death onset that indicates the timing for preventive actions.

This study proposes to develop a real-time monitoring model that quantifies the dying cell percentage in lab-scale bioreactors using capacitance spectroscopy. The capacitance spectroscopy contains cell death-related information due to various physical properties changes during the cell death process, e.g., cytoplasmic conductivity change. The …

A Literature Review Of Wetland Treatment Systems Used To Treat Runoff Mixtures Containing Antibiotics And Pesticides From Urban And Agricultural Landscapes, Emily R. Nottingham, Tiffany L. Messer

Biosystems and Agricultural Engineering Faculty Publications

Wetland treatment systems are used extensively across the world to mitigate surface runoff. While wetland treatment for nitrogen mitigation has been comprehensively reviewed, the implications of common-use pesticides and antibiotics on nitrogen reduction remain relatively unreviewed. Therefore, this review seeks to comprehensively assess the removal of commonly used pesticides and antibiotics and their implications for nitrogen removal in wetland treatment systems receiving non-point source runoff from urban and agricultural landscapes. A total of 181 primary studies were identified spanning 37 countries. Most of the reviewed publications studied pesticides (n = 153) entering wetlands systems, while antibiotics (n = 29) had …

The Rotator Cuff Tendon-To-Bone Interface: Maturation, Aging, And 3d Bioprinting For Regeneration, Xiping Jiang

Theses & Dissertations

Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area with a high prevalence for the elderly population. In addition, regeneration of the gradient structure of the enthesis is still a significant clinical challenge. Our studies aim to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging, and develop a novel therapeutic method using three-dimensional (3D) bioprinting technique to regenerate a functional enthesis. Striking variations of the entheses were observed both histologically and biomechanically during the maturation process. The histological features did not show many differences at the insertion site …

Characterization Of Phase Behavior In Idp Solutions By Uv-Vis Spectrophotometry And Phase Volume Fraction Measurements In Capillaries, Qing Sun

Biomedical Engineering ETDs

Intrinsically disordered proteins (IDPs) play vital roles in cell signaling and regulation by changing conformation dynamically under various stimulus. Thus, it is crucial to understand their physical properties in vitro especially liquid-liquid phase separation (LLPS). Here, we first used UV-vis spectrophotometry to investigate two of IDPs' physical properties – transition temperature (Tt) and coacervate size. This deeper insight allows us to further understand the dynamics of phase growth. We then constructed a binodal curve in the phase diagram by measurement of volume ratios in phase separated IDP solution in glass capillaries.

Synthetic Aperture Optical Imaging Interferometric Microscopy With Improved Image Quality, Preyom K. Dey

Electrical and Computer Engineering ETDs

The resolution limit of optical microscopy can be extended by using Imaging Interferometric Microscopy (IIM), which uses a low numerical aperture (NA) objective lens to achieve resolution equivalent to that of a high-NA objective lens with multiple sub-images. Along with the resolution enhancement challenge, IIM often suffers from poor image quality. In this dissertation, several image quality improvement methods are proposed and verified with simulation and experimental results. Next, techniques to extend the resolution limit of IIM to ≤ 100nm using a low-NA objective lens are demonstrated. An experimental technique of using a grating coupler on …

Advancing Cell-Free Protein Synthesis Systems For On-Demand Next-Generation Protein Therapeutics And Clinical Diagnostics, Emily Ann Long Zhao

Theses and Dissertations

Recombinant proteins have many medical and industrial applications, but their use is complicated by commercial production and stability constraints. These issues are particularly challenging for recombinant proteins used in pharmaceutical therapeutics and clinical diagnostics. Expensive production and distribution limit the accessibility of therapeutics and diagnostics especially in the developing world. Additionally, clinical use of recombinant proteins face further challenges within biological systems including biological degradation and immunogenicity. To increase the accessibility of recombinant proteins, the cost and inefficiencies of protein manufacturing and distribution need to be significantly reduced. A powerful tool to aid in this endeavor is cell-free protein synthesis …

Noise And Time Pressure Effects On Situation Awareness And Aviation Maintenance Tasks, Syaza R. M. Haris

Doctoral Dissertations and Master's Theses

Aircraft maintenance technicians (AMTs) working in a line maintenance work setting are very susceptible to the deafening occupational noise from the airport vicinity or the maintenance machinery itself. Compared to a base maintenance working period, a line maintenance job requires AMTs to complete a task within a short time frame. The current study's objective is to determine if different noise levels and time pressure influence AMTs' performance and situation awareness (SA). Sixteen Embry-Riddle Aeronautical University students majoring in Aviation Maintenance Science participated in a within-subject experimental design. Each participant's performance, SA level, and perceived workload were measured during maintenance tasks …

Low-Level Groundwater Atrazine In High Atrazine Usage Nebraska Counties: Likely Effects Of Excessive Groundwater Abstraction, Moses New-Aaron, Olufemi P. Abimbola, Raheleh Mohammadi, Oluwaseun Famojuro, Zaeema Naveed, Azar M Abadi, Jesse E. Bell, Shannon Bartelt-Hunt, Eleanor G. Rogan

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Recent studies observed a correlation between estrogen-related cancers and groundwater atrazine in eastern Nebraska counties. However, the mechanisms of human exposure to atrazine are unclear because low groundwater atrazine concentration was observed in counties with high cancer incidence despite having the highest atrazine usage. We studied groundwater atrazine fate in high atrazine usage Nebraska counties. Data were collected from Quality Assessed Agrichemical Contaminant Nebraska Groundwater, Parameter–Elevation Regressions on Independent Slopes Model (PRISM), and water use databases. Descriptive statistics and cluster analysis were performed. Domestic wells (59%) were the predominant well type. Groundwater atrazine was affected by well depth. Clusters consisting …

Development And Application Of A Novel Acoustic Microfluidic Technology For Single Cell Per Well Trapping And High-Resolution Analysis Of Cilia Motion In Chlamydomonas Reinhardtii, Mingyang Cui

McKelvey School of Engineering Graduate Student Theses & Dissertations

Acoustic manipulation of cells and microorganisms is a label-free and contact-free technique with promise for biological and biomedical applications. When exposed to an ultrasonic standing wave field, particles suspended in microfluidic channels will be moved to pressure minima (nodes) or maxima (antinodes) due to the acoustic impedance mismatch between particles and the suspension medium. Cilia motion is fundamental to understanding biological and biomedical problems related to dysfunctional human cilia, including primary ciliary dyskinesia, blindness, and male infertility. However, in vivo and ex vivo mammalian ciliated cell research is laborious and time-consuming due to difficulty in growing, maintaining, and imaging these …

Identifying Structures For Ion Channel Kinetic Models, Kathryn Mangold

McKelvey School of Engineering Graduate Student Theses & Dissertations

Voltage-gated ion channels carry transmembrane current and underlie the voltages changes seen during the action potential. The ion channels open and close precisely, or gate, in response to voltage to create the specific cellular action potential waveform. The first models of voltage-gated ion channels gating dynamics were described by Hodgkin and Huxley when studying the neuronal action potential in the squid giant axon. While the probabilistic gating variables cleverly imagined by Hodgkin and Huxley to describe channel gating are still used today to describe cardiac ionic currents, Markov (or state-dependent) models provide a more accurate representation of channel dynamics. As …

Zebrafish Models Of Katp Channelopathies, Soma S. Singareddy

McKelvey School of Engineering Graduate Student Theses & Dissertations

Much work has been carried out to understand the composition, structure, and function of ATP-sensitive potassium (KATP) channels in mammalian tissues, as well as the molecular basis of the channelopathies that result from loss-of-function or gain-of-function mutations in these channels. These studies have also highlighted that the available pharmacology to treat such diseases is limited. To overcome such limitations, a better understanding of the tissue specificity of expression, the exact mechanisms linking the molecular dysfunction to the development of complex pathophysiology, the potential for reducing cross-reactivity of drugs, and ultimately more specific therapies are needed. The work described in this …

Laser Stimulated Dynamic Thermal Imaging System For Tumor Detection, Hongyu Meng

McKelvey School of Engineering Graduate Student Theses & Dissertations

Laser stimulated dynamic thermal imaging system for tumor detectionby Hongyu Meng Doctor of Philosophy in Biomedical Engineering Washington University in St. Louis, 2021 Professor Samuel Achilefu, Chair Recent advances in infrared sensor technology have enabled the rapid application of thermal imaging in materials science, security and medicine. Relying on the infrared characteristics of living systems, thermal imaging has been used to generate individual heat maps, detect inflammation and tumor. As an imaging system, thermal imaging has the advantages of portability, real-time, non-invasive, and non-contact. But the low specificity of thermal imaging hinders its wide clinical application.

Unfortunately, label-free DTI is …

Anion Exchange Membranes In Electrochemical Energy Systems, Yunzhu Zhang

McKelvey School of Engineering Graduate Student Theses & Dissertations

Non-CO2-emitting sources of renewable energy, such as solar and wind, have become quite popular in recent years, and their electricity generation cost at scale has become comparable with that of gas- and coal-fired power plants. However, their intermittent output makes the resultant electricity hard to dispatch in a reliable manner in the absence of grid-scale electrical energy storage (EES) technologies. Redox-flow batteries (RFBs) are promising devices for medium- to large-scale electric energy storage. They are safe, reliable, and portable, and are designed with decoupled power and energy modules, which makes it easy and cost-effective to scale their output to meet …

Short-Term Memory And Olfactory Signal Processing, Lijun Zhang

McKelvey School of Engineering Graduate Student Theses & Dissertations

Modern neural recording methodologies, including multi-electrode and optical recordings, allow us to monitor the large population of neurons with high temporal resolution. Such recordings provide rich datasets that are expected to understand better how information about the external world is internally represented and how these representations are altered over time. Achieving this goal requires the development of novel pattern recognition methods and/or the application of existing statistical methods in novel ways to gain insights into basic neural computational principles. In this dissertation, I will take this data-driven approach to dissect the role of short-term memory in olfactory signal processing in …

Acoustic Radiation Force And Its Application For Cell Manipulation And Ion Channels Activation, Xiangjun Peng

McKelvey School of Engineering Graduate Student Theses & Dissertations

Sound is a stress wave that carries energy and momentum flux. Scattered sound waves can generate acoustic radiation force that can be used to manipulate particles or cells. This dissertation demonstrates the physics behind cell manipulation by ultrasound. The work begins with a detailed analysis of the mechanics of using standing surface acoustic waves to fabricate acoustic tweezers for contactless particle manipulation using acoustic radiation force. Models to design and analyze acoustic radiation force have traditionally relied on plane wave theories that cannot predict how standing surface acoustic waves can levitate cells in the direction perpendicular to the substrate. We …

Photoacoustic Imaging Of Colorectal Cancer And Ovarian Cancer, Xiandong Leng

McKelvey School of Engineering Graduate Student Theses & Dissertations

Photoacoustic (PA) imaging is an emerging hybrid imaging technology that uses a short-pulsed laser to excite tissue. The resulting photoacoustic waves are used to image the optical absorption distribution of the tissue, which is directly related to micro-vessel networks and thus to tumor angiogenesis, a key process in tumor growth and metastasis. In this thesis, the acoustic-resolution photoacoustic microscopy (AR-PAM) was first investigated on its role in human colorectal tissue imaging, and the optical-resolution photoacoustic microscopy (OR-PAM) was investigated on its role in human ovarian tissue imaging.Colorectal cancer is the second leading cause of cancer death in the United States. …

Deep Learning For Automatic Microscopy Image Analysis, Shenghua He

McKelvey School of Engineering Graduate Student Theses & Dissertations

Microscopy imaging techniques allow for the creation of detailed images of cells (or nuclei) and have been widely employed for cell studies in biological research and disease diagnosis in clinic practices.Microscopy image analysis (MIA), with tasks of cell detection, cell classification, and cell counting, etc., can assist with the quantitative analysis of cells and provide useful information for a cellular-level understanding of biological activities and pathology. Manual MIA is tedious, time-consuming, prone to subject errors, and are not feasible for the high-throughput cell analysis process. Thus, automatic MIA methods can facilitate all kinds of biological studies and clinical tasks. Conventional …

A Case Study On Hls Portability From Intel To Xilinx Fpgas, Zhili Xiao

McKelvey School of Engineering Graduate Student Theses & Dissertations

Field-programmable gate arrays (FPGAs) are a hardware accelerator option that is growing in popularity. However, FPGAs are notoriously hard to program. To this end, high-level synthesis (HLS) tools have been developed to allow programmers to design hardware accelerators with FPGAs using familiar software languages. The two largest FPGA vendors, Intel and Xilinx, support both C/C++ and OpenCL C to construct kernels. However, little is known about the portability of designs between these two platforms. In this work, we evaluate the portability and performance of Intel and Xilinx kernels. We conduct a case study, porting the Needleman-Wunsch application from the Rodinia …