Digital Commons Network^™

Graphene Oxide Functionalized Biosensor For Detection Of Stress-Related Biomarkers, Erican Santiago, Shailu Shree Poudyal, Sung Y. Shin, Hyeun Joong Yoon

Michigan Tech Publications, Part 1

A graphene oxide (GO)-based cortisol biosensor was developed to accurately detect corti-sol concentrations from sweat samples at point-of-care (POC) sites. A reference electrode, counter electrode, and working electrode make up the biosensor, and the working electrode was functional-ized using multiple layers consisting of GO and antibodies, including Protein A, IgG, and anti-Cab. Sweat samples contact the anti-Cab antibodies to transport electrons to the electrode, resulting in an electrochemical current response. The sensor was tested at each additional functionalization layer and at cortisol concentrations between 0.1 and 150 ng/mL to determine how the current response differed. A potentiostat galvanostat device was …

Fabrication And Characterization Of A Ph-Sensitive Intelligent Film Incorporating Dragon Fruit Skin Extract, Nurnabila Afiqah Azlim, Abdorreza Mohammadi Nafchi, Nazila Oladzadabbasabadi, Fazilah Ariffin, Pantea Ghalambor, Shima Jafarzadeh, A. A. Al-Hassan

Research outputs 2022 to 2026

A novel intelligent pH-sensing indicator based on gelatin film and anthocyanin extracted from dragon fruit skin (Hylocereus polyrhizus) (DFSE) as a natural dye was developed to monitor food freshness by the casting method. Anthocyanin content of DFSE was 15.66 ± 1.59 mg/L. Dragon fruit bovine gelatin films were characterized by Fourier transform infrared spectroscopy (FTIR) and observed by a scanning electron microscope (SEM). Moisture content, mechanical properties, water solubility, water vapor permeability (WVP), light transmittance, color, and pH-sensing evaluations were evaluated for potential application. FTIR spectroscopy revealed that the extracted anthocyanin could interact with the other film components through hydrogen …

C2c Stem Collaboration, Mary Andrea Parish, Angela Renee Rowe, Annette Hines, Hannah Elizabeth Brewer

Posters-at-the-Capitol

Campus to Classroom is a STEM collaboration effort brought about by the brainstorming and continued efforts of Ms. Angela Rowe (MSUTeach), Ms. Annette Hines (Talent Search), MSUTeach STEM Ambassadors, and MSUExplore (MSUTeach student group).

The goal is an educational, collaborative partnership with cross-campus departments and both pre-service and in-service teachers, with a main focus on students from underserved population and communities.

Continued steps will be planned to develop a multi-level partnership with the end goals of increased usage of project-based instruction, incorporate research that increases STEM knowledge/skill, and encourage career exploration while building bridges from “campus and classroom”.

Magnetic Nanoparticles Enhanced Surface Plasmon Resonance Biosensor For Rapid Detection Of Salmonella Typhimurium In Romaine Lettuce, Devendra Bhandari, Fur-Chi Chen, Roger C. Bridgman

Human Sciences Faculty Research

Salmonella is one of the major foodborne pathogens responsible for many cases of illnesses, hospitalizations and deaths worldwide. Although different methods are available to timely detect Salmonella in foods, surface plasmon resonance (SPR) has the benefit of real-time detection with a high sensitivity and specificity. The purpose of this study was to develop an SPR method in conjunction with magnetic nanoparticles (MNPs) for the rapid detection of Salmonella Typhimurium. The assay utilizes a pair of well-characterized, flagellin-specific monoclonal antibodies; one is immobilized on the sensor surface and the other is coupled to the MNPs. Samples of romaine lettuce contaminated with …

The Use Of Microalgal Sourced Biodiesel To Help Underground Mines Transition To Battery Electric Vehicles, Corey A. Laamanen, Kyle Moreau, Sabrina M. Desjardins, Shannon H. Mclean, John A. Scott

Journal of Sustainable Mining

The use of fossil fuel sourced diesel underground has various associated health and environmental hazards, and additional energy demand and costs associated with necessary ventilation. One way to reduce these impacts is by utilizing a biodieselblend, which generates lower levels of harmful emissions from underground equipment and can be produced regionally, reducing the impact of transportation. Furthermore, this would help allow use of existing machinery during transition towards more widespread electrification underground. Therefore, the concept of an integrated supply and use chain within the mining industry is examined based on biodiesel from acidophilic photosynthetic microalgae cultivated using CO 2 in …

Application Of Entropy Method For Estimating Factor Weights In Mining-Method Selection For Development Of Novel Mining-Method Selection System, Elsa Pansilvania Andre Manjate, Mahdi Saadat, Hisatoshi Toriya, Fumiaki Inagaki, Youhei Kawamura

Journal of Sustainable Mining

Mining-method selection (MMS) is one of the most critical and complex decisionmaking processes in mine planning. Therefore, it has been a subject of several studies for many years culminating with the development of different systems. However, there is still more to be done to improve and/or create more efficient systems and deal with the complexity caused by many influencing factors. This study introduces the application of the entropy method for feature selection, i.e., select the most critical factors in MMS. The entropy method is applied to assess the relative importance of the factors influencing MMS by estimating their objective weights …

Role Of Inflammatory Niche And Adult Cardiomyocyte Coculture On Differentiation, Matrix Synthesis, And Secretome Release By Human Bone Marrow Mesenchymal Stem Cells, Jyotsna Joshi, Chandrasekhar R. Kothapalli

Chemical & Biomedical Engineering Faculty Publications

Myocardial infarction (MI) causes cardiomyocyte death, provokes innate immune response, and initiates tissue remodeling. The intrinsic healing process is insufficient to replace the lost cells, or regenerate and restore the functional features of the native myocardium. Autologous bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation is being explored to offer therapeutic potential after MI. Here, we cultured human BM-MSC spheroids in three-dimensional collagenous gels for 28 days under exposure to tumor necrosis factor-alpha (+ TNFα), and coculture with adult human cardiomyocytes, or with conditioned media (CM) pooled from TNFα-stimulated adult cardiomyocytes. MSC differentiation marker (CD90, GATA4, cTnI, cTnT, Cx43, MHC, α-actin) …

Rapid Response: Cat-Scan Machine And 3d Printers Vital In Covid-19, Laura Meader

Colby Magazine

Colby’s Assistant Professor of Biology Josh Martin is using his expertise and sophisticated scientific equipment to create face shields, respirator masks, and air filter cartridges for local first responders and hospitals in need of equipment that is crucial to treating patients with COVID-19.

Glycocalyx Mechanotransduction Mechanisms Are Involved In Renal Cancer Metastasis, Heriberto Moran, Limary M. Cancel, Peigen Huang, Sylvie Roberge, Tuoye Xu, John M. Tarbell, Lance L. Munn

Publications and Research

Mammalian cells, including cancer cells, are covered by a surface layer containing cell bound proteoglycans, glycoproteins, associated glycosaminoglycans and bound proteins that is commonly referred to as the glycocalyx. Solid tumors also have a dynamic fluid microenvironment with elevated interstitial flow. In the present work we further investigate the hypothesis that interstitial flow is sensed by the tumor glycocalyx leading to activation of cell motility and metastasis. Using a highly metastatic renal carcinoma cell line (SN12L1) and its low metastatic counterpart (SN12C) we demonstrate in vitro that the small molecule Suberoylanilide Hydroxamic Acid (SAHA) inhibits the heparan sulfate synthesis enzyme …

Applications Of Nano-Zeolite In Wastewater Treatment: An Overview, Rehab O, Abdel Rahman, Ahmed M. El-Kamash, Yung-Tse Hung

Civil and Environmental Engineering Faculty Publications

Nano-zeolite is an innovative class of materials that received recognition for its potential use in water and tertiary wastewater treatment. These applications include ion-exchange/sorption, photo-degradation, and membrane separation. The aim of this work is to summarize and analyze the current knowledge about the utilization of nano-zeolite in these applications, identify the gaps in this field, and highlight the challenges that face the wide scale applications of these materials. Within this context, an introduction to water quality, water and wastewater treatment, utilization of zeolite in contaminant removal from water was addressed and linked to its structure and the advances in zeolite …

A Qualitative Single-Case Exploring The Impact Of A Mentor And Cohort On Students’ Academic And Career Decisions., Kevin Adkins, Samantha Bowyer, Jorge L. D. Albelo Ph.D.

Publications

Exposure to research is known to play a positive role in undergraduate education. However, robust research responsibilities are oftentimes not formally incorporated into a student’s academic experience until master level studies. Further, a variety of intimidation factors often inhibit many undergraduate students from participating on a research team. In this case study, the research team evaluated a unique group of undergraduate researchers who matriculated to the same university after beginning to participate in research as concurrently enrolled students. Following matriculation, each student continued to participate in research throughout the entirety of their undergraduate studies. All of the study’s students were …

Detecting The Feeding Habitat Zone Of Albacore Tuna (Thunnus Alalunga) In The Southern Indian Ocean Using Multisatellite Remote Sensing Data, Sandipan Mondal, Yang-Chi Lan, Ming-An Lee, Yi-Chen Wang, Bambang Semedi, Wan-Ya Su

Journal of Marine Science and Technology–Taiwan

Remote sensing is an effective method for identifying potential feeding habitat zones. Spatial and temporal distribution patterns of albacore (ALB) tuna were studied using Taiwan longline fisheries data from 2009 to 2014. A generalized additive model (GAM) was used to compile a fishery database and statistically explore the relationship between distribution and environmental factors. Sea surface temperature (SST), sea surface chlorophyll-a concentration (SSC), sea surface salinity (SSS), sea surface height (SSH), mixed layer depth (MLD), and eddy kinetic energy (EKE) were examined. The results indicated SST as the largest contributor on ALB distribution, followed by SSS and SSC. Catches per …

Japanese Consumer Preference For Raw Fish: Best–Worst Scaling Method, Takahiro Sajiki, Yu-Heng Lu

Journal of Marine Science and Technology–Taiwan

Given the declining consumption of fish and shellfish in Japan, it is essential to better understand consumer needs to design policies that increase consumption. Therefore, we clarify the relative degree of importance Japanese consumers place on various attributes of raw fish during purchase based on a questionnaire survey employing best–worst scaling. We find that consumers prioritize freshness, price, taste, appearance, safety, seasonality, ease of cooking and eating, nutrition, origin, wild fish, and effect on natural environment and ecosystem, in that order. Wild fish has unexpectedly low importance, although Japanese consumers conventionally purchase captured fishery products rather than cultured ones. The …

Diel Vertical Distribution Patterns Of Pelagic Fish Larvae In Yilan Bay, Taiwan, Yi-Chen Wang, Su-Chen Tsai, Wen-Yu Chen

Journal of Marine Science and Technology–Taiwan

To study the diel migration distribution of larval fish in an area adjacent to the Kuroshio Current, samples were collected from six depths by using a Motoda horizontal net at a fixed station in Yilan Bay on July 30 and 31, 2007. A total of 591 larval fish were collected and assigned to at least 34 families and 57 species. The first five dominant species were Benthosama pterotum (40.95%), Diaphus B type (12.18%), Callionymidae spp. (8.46%), Gobiidae spp. (6.60%), and Apogonidae spp. (4.0%). The density of larval fish varied across time. The maximum larval density was 1220.34 individuals/1000 m3 at …

Long-Term Variation Of Sea Surface Temperature In Relation To Sea Level Pressure And Surface Wind Speed In Southern Indian Ocean, Sandipan Mondal, Ming-An Lee, Yi-Chen Wang, Bambang Semedi

Journal of Marine Science and Technology–Taiwan

Sea surface temperature (SST) is an essential parameter associated with fish habitat and changes in oceanic conditions. Long-term SST variation in relation to sea level pressure (SLP) and surface wind speed (SWS) was observed based on the National Oceanic and Atmospheric Administration (NOAA) data of southern Indian Ocean. Mean monthly time series showed that February and November were the warmest and coolest months, respectively. Spatial distribution showed that 0°S–30°S was warm with SST of 20°–30°C, which was higher than that at all other places throughout the year. SLP (> 1010 millibar) and SWS (> 12 m/s) were high at 20°S–40°S …

Economic Conditions For New Entrants In Coastal Fishing In Miyazaki Prefecture, Japan, Takahiro Sajiki, Yu-Heng Lu, Norie Nishiguchi, Sonomi Akamine, Masaharu Nishiguchi

Journal of Marine Science and Technology–Taiwan

A significant decrease in the number of fishery workers is be-coming a problem in Japan. For new entrants to become estab-lished in the region, they must be profitable as fishing busi-nesses. Using the case of Miyazaki prefecture in Japan, this paper identifies the economic conditions required to make longline fishing and bottom gillnet fishing profitable by ap-plying the net present value (NPV) method. When operating on 200 days, which is the guideline for new entrants in longline fishing, if the value of investment is less than 44.1% of the minimum required, the investment will not provide positive returns. However, the …

Raw Garlic (Allium Sativum) Improves Nonspecific Immune Responses And Resistance Against Vibrio Alginolyticus Infection In Grouper (Epinephelus Coioides), Huai-Ting Huang, Po-Tsang Lee, Zhen-Hao Liao, Cheng-Ting Huang, Hsiang-Yin Chen, Zhi-Gu Huang, Fan-Hua Nan, Yu-Sheng Wu

Journal of Marine Science and Technology–Taiwan

Garlic contains various nutrients and healthful ingredients. The content of garlic products is closely related to their processing methods, and each compound has specific biological effects. Allicin, a heat labile compound, is considered the most important factor in garlic. Garlic powder is commonly prepared by subjecting raw garlic to thermal processing before being grounded into powder,and it is of interest to compare the biological activity of garlic powder with raw garlic in fish. This study investigated immune effects of raw garlic and garlic powder on groupers (Epinephelus coioides). Although raw garlic and garlic powder did not promote growth performance of …

Asymptotic Numerical Solutions For Second-Order Quasilinear Singularly Perturbed Problems, Chein-Shan Liu, Chih-Wen Chang

Journal of Marine Science and Technology–Taiwan

For a second-order quasilinear singularly perturbed problem under the Dirichlet boundary conditions, we propose a new asymptotic numerical method, which involves two problems: a reduced problem with a one-side boundary condition and a novel boundary layer correction problem with a two-sided boundary condition. Through the introduction of two new variables, both problems are transformed to a set of three first-order initial value problems with zero initial conditions. The Runge–Kutta method is then applied to integrate the differential equations and to determine two unknown terminal values of the new variables until they converge. The modified asymptotic numerical solution satisfies the Dirichlet …

Simulation Of A Ship And Tension Leg Platform Wind Turbine Collision, Jiamin Guo, Yu Zhao, Weigang Chen, Guangeng Zhou

Journal of Marine Science and Technology–Taiwan

Collisions between ships and tension leg platform wind turbines (TLPWTs) result in the TLPWT damage, including tower damage and tension leg breakage. This paper details a collision between a ship and TLPWT simulated using the LS-DYNA software package. During simulation, the behaviors of the water around the ship and of the TLPWT were processed using the constant added mass and the fluid–structure interaction methods, respectively. The results indicate that the energy conversion during collision is generally consistent with the law of conversion of energy, suggesting that the simulation’s calculations are acceptable. The TLPWT simulation results imply that two distinct collisions …

The Influences Of Promising Feedstock Variability On Advanced Biofuel Production: A Review, Cherng-Yuan Lin

Journal of Marine Science and Technology–Taiwan

The contribution of biofuels is expected to continuously increase in the global fuel market, as they are environmentally-friendly and provide renewable energy. Four generations of biofuels are categorized and are primarily based on their feedstock sources and the production technologies that are used. The influence of promising feedstock types and the availability on the production technologies and the fuel properties of advanced-generation biofuels are not systematically examined in the literature. Hence, this research extensively reviews the potential impact of feedstock sources and their variability on the production and characteristics of biofuels. The approaches of theoretical analysis and inference referred to …

Toward A Multimodal Computer-Aided Diagnostic Tool For Alzheimer’S Disease Conversion, Danilo Pena, Jessika Suescun, Mya Schiess, Timothy M. Ellmore, Luca Giancardo, Alzheimer’S Disease Neuroimaging Initiative

Publications and Research

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder. It is one of the leading sources of morbidity and mortality in the aging population AD cardinal symptoms include memory and executive function impairment that profoundly alters a patient’s ability to perform activities of daily living. People with mild cognitive impairment (MCI) exhibit many of the early clinical symptoms of patients with AD and have a high chance of converting to AD in their lifetime. Diagnostic criteria rely on clinical assessment and brain magnetic resonance imaging (MRI). Many groups are working to help automate this process to improve the clinical workflow. Current …

Centrifugal Assembly Of Bijel Ropes Via Helical Microfluidics, Shankar P. Kharal

Theses and Dissertations

Bicontinuous interfacially jammed emulsion gels (bijels) are soft materials that retain a liquid bicontinuous network stabilized by an interfacially jammed layer of nanoparticles. In this thesis, we investigated a microfluidic twisting method to fabricate micro-ropes of nano-structured bijel fibers. This method shows how weak microfibers with tensile strengths of a few kPa can be reinforced by 4 orders of magnitude by means of microfluidic twisting. Microfluidic twisting allows to produce continuous bijel fiber ropes of controllable architecture. Modelling the fluid flow field reveals the rope geometry dependence on a subtle force balance composed of rotational and translational shear stresses. However, …

Stress Corrosion Analysis And Direct Cell Viability Of Biodegradable Zn-Fe-Ca Alloy In In-Vitro Conditions, Orit Avior, Noa Ben Ghedalia-Peled, Tomer Ron, Jeremy Goldman, Razi Vago, Eli Aghion

Michigan Tech Publications, Part 1

Due to the excellent biocompatibility of Zn and Zn-based alloys, researchers have shown great interest in developing biodegradable implants based on zinc. Furthermore, zinc is an essential component of many enzymes and proteins. The human body requires ~15 mg of Zn per day, and there is minimal concern for systemic toxicity from a small zinc-based cardiovascular implant, such as an arterial stent. However, biodegradable Zn-based implants have been shown to provoke local fibrous encapsulation reactions that may isolate the implant from its surrounding environment and interfere with implant function. The development of biodegradable implants made from Zn-Fe-Ca alloy was designed …

Chaotic Dynamics In The 2d System Of Nonsmooth Ordinarydifferential Equations, Zain-Aldeen S. A. Rahman, Basil H. Jasim, Yasir I. A. Al-Yasir

Iraqi Journal for Computer Science and Mathematics

Over the last decade, the chaotic behaviors of dynamical systems have been extensively explored.Recently, discovering or developing a 2D system of ordinary differential equations (ODEs) capable of exhibitingchaotic dynamical behaviors is an attractive research topic. In this study, a chaotic system with a 2D system ofnonsmooth ODEs has been developed. This system is can exhibit chaotic dynamical behaviors. Its main dynamicalbehaviors, including time-series trajectories, phase portraits of attractors, and equilibria and their stability, have beeninvestigated. The developed system has been verified by an excessive variety of fascinating chaotic behaviors, such aschaotic attractor, symmetry, sensitivity to initial conditions (ICs), fractal dimension, …

Genome Structure And Evolutionary History Of Frankincense Producing Boswellia Sacra, Abdul Latif Khan, Ahmed Al-Harrasi, Jin-Peng Wang, Sajjad Asaf, Jean-Jack Riethoven, Tariq Shehzad, Chia-Sin Liew, Xiao-Ming Song, Daniel P. Schachtman, Chao Liu, Ji-Gao Yu, Zhi-Kang Zhang, Fan-Bo Meng, Jia-Qing Yuan, Chen-Dan Wei, He Guo, Xuewen Wang, Ahmed Al-Rawahi, In-Jung Lee, Jeffrey L. Bennetzen, Xi-Yin Wang

Nebraska Center for Biotechnology: Faculty and Staff Publications

Boswellia sacra Flueck (family Burseraceae) tree is wounded to produce frankincense. We report its de novo assembled genome (667.8 Mb) comprising 18,564 high-confidence protein-encoding genes. Comparing conserved single-copy genes across eudicots suggest >97% gene space assembly of B. sacra genome. Evolutionary history shows B. sacra gene-duplications derived from recent paralogous events and retained from ancient hexaploidy shared with other eudicots. The genome indicated a major expansion of Gypsy retroelements in last 2 million years. The B. sacra genetic diversity showed four clades intermixed with a primary genotype—dominating most resin-productive trees. Further, the stemtranscriptome revealed that wounding concurrently activates phytohormones signaling, …

Characterizing Isoform Switching Events In Esophageal Adenocarcinoma, Yun Zhang, Katherine M. Weh, Connor L. Howard, Jean-Jack Riethoven, Jennifer L. Clarke, Kiran H. Lagisetty, Jules Lin, Rishindra M. Reddy, Andrew C. Chang, David G. Beer, Laura A. Kresty

Nebraska Center for Biotechnology: Faculty and Staff Publications

Isoform switching events with predicted functional consequences are common in many cancers, but characterization of switching events in esophageal adenocarcinoma (EAC) is lacking. Next-generation sequencing was used to detect levels of RNA transcripts and identify specific isoforms in treatment- naïve esophageal tissues ranging from premalignant Barrett’s esophagus (BE), BE with low- or high-grade dysplasia (BE.LGD, BE.HGD), and EAC. Samples were stratified by histopathology and TP53 mutation status, identifying significant isoform switching events with predicted functional consequences. Comparing BE.LGD with BE.HGD, a histopathology linked to cancer progression, isoform switching events were identified in 75 genes including KRAS, RNF128, and WRAP53. Stratification …

Active Packaging Film Based On Poly Lactide-Poly (Butylene Adipate-Co-Terephthalate) Blends Incorporated With Tannic Acid And Gallic Acid For The Prolonged Shelf Life Of Cherry Tomato, Shubham Sharma, Kalpani Perera, Dileswar Pradhan, Brendan Duffy, Amit Jaiswal, Swarna Jaiswal

Articles

The antimicrobial property is the key feature of active packaging. Biological macromolecules such as tannic and gallic acids are naturally found in plants such as tea, fruits, berries, and grapes. The incorporation of tannic acid (TA) and gallic acid (GA) in the biodegradable polymer blend Poly Lactide-Poly (Butylene Adipate-Co-Terephthalate) (PLA-PBAT) was used in this study to assess the potential of active packaging. TA and GA (10 wt%) composite films showed a 65%–66% increase in the UV barrier property. The tensile strength value increased after the incorporation of TA and GA (10 wt%), respectively. Overall, 1.67 and 2.2 log reductions in …

Analysis Of Human Gait Cycle With Body Equilibrium Based On Leg Orientation, Muhammad Asif, Muhammad Abdullah Tayyab, Muhammad Hammad Shahid, Usama Arif, Mohsin Islam Tiwana, Umar Shahbaz Khan, Waqar Shahid Qureshi

Articles

Gait analysis identifies the posture during movement in order to provide the correct actions for a normal gait. A person's gait may differ from others and can be recognized by specific patterns. Healthy individuals exhibit normal gait patterns, while lower limb amputees exhibit abnormal gait patterns. To better understand the pitfalls of gait, it is imperative to develop systems capable of capturing the gait patterns of healthy individuals. The main objective of this research was to introduce a new concept in gait analysis by computing the static and dynamic equilibrium in a real-world environment. A relationship was also presented among …

A Combined Electrochemical-Biological System For The Production Of Liquid Fuel From Co2, Elizabeth Hann

Link Foundation Energy Fellowship Reports

Biological photosynthesis is the origin of our liquid transportation fuels, from petroleum to corn ethanol. Biological photosynthesis has an efficiency of approximately 1% for the conversion of photons from sunlight to biomass1. Increasing the energy efficiency of biomass production (solar-to-biomass conversion) would allow for biomass, and biofuels, to be produced using less resources. Artificial photosynthesis seeks to overcome the limitations of biological photosynthesis, including low efficiency of solar energy capture and poor carbon dioxide reduction, and could provide an alternative route for biomass production. A two-step electrochemical process converts CO2 to acetate. Acetate is a soluble, two-carbon substrate that can …

A Greenhouse Gas Emissions Inventory For Nebraska: Livestock And Coal Loom Large, Eric R. Holley, Adam Liska

Adam Liska Papers

Mitigation of climate change requires the systematic identification and cataloging of emissions sources at city, state, and national levels. In this study, an inventory of annual greenhouse gas emissions from the state of Nebraska was created based on industry data, and emissions inventories were completed each year from 1990 to 2016. Nebraska’s net emissions were found to increase from 56.2 million metric tons of carbon dioxide equivalents (MMtCO₂e) in 1990 to 87.4 MMtCO₂e in 2016. Agriculture was found to be the sector with the most emissions (36 MMtCO₂e) followed by electricity generation (21 MMtCO …