Digital Commons Network^™

Histone Deacetylase 4 And 5 Translocation Elicited By Microsecond Pulsed Electric Field Exposure Is Mediated By Kinase Activity, Zahra Safaei, Gary L. Thompson Iii

Henry M. Rowan College of Engineering Departmental Research

Electroporation-based technologies using microsecond pulsed electric field (µsPEF) exposures are established as laboratory and clinical tools that permeabilize cell membranes. We demonstrate a µsPEF bioeffect on nucleocytoplasmic import and export of enzymes that regulate genetic expression, histone deacetylases (HDAC) -4 and -5. Their μsPEF-induced nucleocytoplasmic transport depends on presence and absence of extracellular calcium ions (Ca2+) for both MCF7 and CHO-K1 cells. Exposure to 1, 10, 30 and 50 consecutive square wave pulses at 1 Hz and of 100 µs duration with 1.45 kV/cm magnitude leads to translocation of endogenous HDAC4 and HDAC5. We posit that by eliciting a rise …

Histone Deacetylase 4 And 5 Translocation Elicited By Microsecond Pulsed Electric Field Exposure Is Mediated By Kinase Activity., Zahra Safaei, Gary L. Thompson Iii

Henry M. Rowan College of Engineering Departmental Research

Electroporation-based technologies using microsecond pulsed electric field (µsPEF) exposures are established as laboratory and clinical tools that permeabilize cell membranes. We demonstrate a µsPEF bioeffect on nucleocytoplasmic import and export of enzymes that regulate genetic expression, histone deacetylases (HDAC) -4 and -5. Their μsPEF-induced nucleocytoplasmic transport depends on presence and absence of extracellular calcium ions (Ca

Computer Engineering Education, Marilyn Wolf

School of Computing: Conference and Workshop Papers

Computer engineering is a rapidly evolving discipline. How should we teach it to our students?

This virtual roundtable on computer engineering education was conducted in summer 2022 over a combination of email and virtual meetings. The panel considered what topics are of importance to the computer engineering curriculum, what distinguishes computer engineering from related disciplines, and how computer engineering concepts should be taught.

Science, Technology, Engineering, And Mathematics (Stem) Project-Based Learning (Pbl) Education: A New Mexico Case Study For Equity And Inclusion, Kimberly A. Scheerer

Teacher Education, Educational Leadership & Policy ETDs

This research addresses how student participation in Science, Technology, Engineering, and Mathematics (STEM) project-based learning (PBL) education activities encourages underrepresented minority student achievement in STEM career field trajectories. Seven New Mexico high school counselors and 12 STEM organization personnel were interviewed during this study. Their responses represent the nuanced professional voices where New Mexico public education intersects with STEM student interest and cultural influence.

For students, STEM PBL can foster deep integration across educational disciplines and enhance STEM career trajectory interest and readiness. STEM education converged with PBL methodologies has the ability to leverage community support while broadening student networks. …

Theoretical And Experimental Analyses Of Acoustofluidic Phenomena For Biosensing Applications, Pradipta Kr. Das

USF Tampa Graduate Theses and Dissertations

Acoustofluidics is a well-proven tweezer serving various applications such as particle manipulation, cell trapping and separations, fluid mixing, and bio-sensing. In this thesis, we explored several aspects of acoustofluidics such as acoustic streaming, acoustothermal heating and acoustophoretic manipulations of micron sized objects, in the context of biosensing applications.

In Chapter 2, inner acoustic streaming for second-order fluids has been studied analytically by employing asymptotic expansions for thin Stokes layer and low acoustic Mach number. In addition, a multiple-time-scale approach has been adopted to separate the primary oscillatory flow and the steady acoustic streaming. The study considers two sample cases: (i) …

Secure Cloud-Based Iot Water Quality Gathering For Analysis And Visualization, Soin Abdoul Kassif Baba M Traore, Maria Valero, Amy Gruss

KSU Proceedings on Cybersecurity Education, Research and Practice

Water quality refers to measurable water characteristics, including chemical, biological, physical, and radiological characteristics usually relative to human needs. Dumping waste and untreated sewage is the reason for water pollution and several diseases to the living hood. The quality of water can also have a significant impact on animals and plant ecosystems. Therefore, keeping track of water quality is a substantial national interest. Much research has been done for measuring water quality using sensors to prevent water pollution. In summary, those systems are built based on online and reagent-free water monitoring SCADA systems in wired networks. However, centralized servers, transmission …

Digitally Predicting Protein Localization And Manipulating Protein Activity In Fluorescence Images Using 4d Reslicing Gan, Yang Jiao, Lingkun Gu, Yingtao Jiang, Mo Weng, Mei Yang

Electrical & Computer Engineering Faculty Research

While multi-channel fluorescence microscopy is a vital imaging method in biological studies, the number of channels that can be imaged simultaneously is limited by technical and hardware limitations such as emission spectra cross-talk. One solution is using deep neural networks to model the localization relationship between two proteins so that the localization of one protein can be digitally predicted. Furthermore, the input and predicted localization implicitly reflect the modeled relationship. Accordingly, observing the response of the prediction via manipulating input localization could provide an informative way to analyze the modeled relationships between the input and the predicted proteins.

Investigation Of Flexible And Wearable Devices For The Analysis Of Biological Signals, Muhammad Usman

Theses and Dissertations

The application potential for flexible and wearable electronics in the field of healthcare has received great interest in recent years. Flexible and wearable devices based on soft, biocompatible materials can be comfortably attached to the human skin for continuous health monitoring. In this work, we present two novel wearable bioelectrical impedance analysis (BIA) systems for body composition measurement. First, we developed a wearable ring-based BIA system for body fat monitoring. The wearable ring contains flexible copper electrodes to interface with the human body. The device is tested on 40 healthy volunteers and the results demonstrate a high correlation with an …

Numerical Investigation Of Heat Generation And Accumulation Contributing To Elevated Temperature In Msw Landfills, Alborz Fathinezhad

LSU Doctoral Dissertations

Landfills are complex geostructures which contains organic and inorganic municipal, and in some cases industrial, wastes and are expected to remain operational for long times. Due to the complex nature of physical, chemical, biological, and thermal reactions that carry on within the depths of a landfill, unexpected incidents such as elevated temperatures could become inevitable. While uncommon to happen, elevated temperatures cause health and environmental issues such as odors, rapid settlements, slope instabilities. In addition, elevated temperatures can negatively impact engineered components in composite bottom liners, cover systems, leachate collection, and gas extraction and recovery systems.

Air intrusion into municipal …

Piglet Cardiopulmonary Bypass Induces Intestinal Dysbiosis And Barrier Dysfunction Associated With Systemic Inflammation, Jeffrey D. Salomon, Haowen Qiu, Dan Feng, Jacob Owens, Ludmila Khailova, Suzanne Osorio Lujan, John Iguidbashian, Yashpal S. Chhonker, Daryl J. Murry, Jean-Jack Riethoven, Merry L. Lindsey, Amar B. Singh, Jesse A. Davidson

Nebraska Center for Biotechnology: Faculty and Staff Publications

The intestinal microbiome is essential to human health and homeostasis, and is implicated in the pathophysiology of disease, including congenital heart disease and cardiac surgery. Improving the microbiome and reducing inflammatory metabolites may reduce systemic inflammation following cardiac surgery with cardiopulmonary bypass (CPB) to expedite recovery postoperatively. Limited research exists in this area and identifying animal models that can replicate changes in the human intestinal microbiome after CPB is necessary. We used a piglet model of CPB with two groups, CPB (n=5) and a control group with mechanical ventilation (n=7), to evaluate changes to the microbiome, …

Material Characterization And Comparison Of Sol-Gel Deposited And Rf Magnetron Deposited Lead Zirconate Titanate Thin Films, Katherine Lynne Miles

Mechanical Engineering ETDs

Lead zirconate titanate (PZT) has been a material of interest for sensor, actuator, and transducer applications in microelectromechanical systems (MEMS). This is due to their favorable piezoelectric, pyroelectric and ferroelectric properties. While various methods are available to deposit PZT thin films, radio frequency (RF) magnetron sputtering was selected to provide high quality PZT films with the added capability of batch processing. These sputter deposited PZT films were characterized to determine their internal film stress, Young’s modulus, composition, and structure. After characterization, the sputtered PZT samples were poled using corona poling and direct poling methods. As a means of comparison, commercially …

Canopycam – An Edge-Computing Sensing Unit For Continuous Measurement Of Canopy Cover Percentage Of Dry Edible Beans, Wei-Zhen Liang, Joseph Oboamah, Xin Qiao, Yufeng Ge, Robert M. Harveson, Daran Rudnick, Jun Wang, Haishun Yang, Angie Gradiz

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Canopy cover (CC) is an important indicator for crop development. Currently, CC can be estimated indirectly by measuring leaf area index (LAI) using commercially available hand-held meters. However, it does not capture the dynamics of CC. Continuous CC monitoring is essential for dry edible beans production since it can affect crop water use, weed, and disease control. It also helps growers to closely monitor “yellowness”, or senescence of dry beans to decide proper irrigation cutoff timing to allow the crop to dry down for harvest. Therefore, the goal of this study was to develop a device – CanopyCAM, containing software …

G-Dam: A Distributed Data Storage With Blockchain Framework For Management Of Groundwater Quality Data, Sukrutha L. T. Vangipuram, Saraju P. Mohanty, Elias Kougianos, Chittaranjan Ray

Department of Civil and Environmental Engineering: Faculty Publications

Groundwater overuse in different domains will eventually lead to global freshwater scarcity. To meet the anticipated demands, many governments worldwide are employing innovative and traditional techniques for forecasting groundwater availability by conducting research and studies. One challenging step for this type of study is collecting groundwater data from different sites and securely sending it to the nearby edges without exposure to hacking and data tampering. In the current paper, we send raw data formats from the Internet of Things to the Distributed Data Storage (DDS) and Blockchain (BC) edges. We use a distributed and decentralized architecture to store the statistics, …

Machine Learning With Gradient-Based Optimization Of Nuclear Waste Vitrification With Uncertainties And Constraints, Lagrande Gunnell, Kyle Manwaring, Xiaonan Lu, Jacob Reynolds, John Vienna, John Hedengren

Faculty Publications

Gekko is an optimization suite in Python that solves optimization problems involving mixed-integer, nonlinear, and differential equations. The purpose of this study is to integrate common Machine Learning (ML) algorithms such as Gaussian Process Regression (GPR), support vector regression (SVR), and artificial neural network (ANN) models into Gekko to solve data based optimization problems. Uncertainty quantification (UQ) is used alongside ML for better decision making. These methods include ensemble methods, model-specific methods, conformal predictions, and the delta method. An optimization problem involving nuclear waste vitrification is presented to demonstrate the benefit of ML in this field. ML models are compared …

The Smart Dementia Care Project, Dympna O'Sullivan, Jonathan Turner, Ciaran Nugent, Damon Berry, Michael Wilson, Julie Doyle

Articles

No abstract provided.

Spatiotemporal Metabolic Network Models Reveal Complex Autotroph-Heterotroph Biofilm Interactions Governed By Photon Incidences, Poonam Phalak, Hans C. Bernstein, Stephen R. Lindemann, Ryan S. Renslow, Dennis G. Thomas, Michael A. Henson, Hyun-Seob Song

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Autotroph-heterotroph interactions are ubiquitous in natural environment and play a key role in controlling various essential ecosystem functions, such as production and utilization of organic matter, cycling of nitrogen, sulfur, and other chemical elements. Understanding how these biofilm metabolic interactions are constrained in space and time remains challenging because fully predictive models designed for this purpose are currently limited. Toward filling this gap, here we developed community metabolic network models for two autotroph-heterotroph biofilm consortia (termed UCC-A and UCC-O), which share a suite of common heterotrophic members but have a single distinct photoautotrophic cyanobacterium (Phormidesmis priestleyi str. ANA and …

Key To The Species Of Tortanidae Tortanus Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Tharybidae Neoscolecithrix Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Temoridae Temora Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Subeucalanidae Subeucalanus Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Spinocalanidae Spinocalanus Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Spinocalanidae Monacilla Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Scolecitrichidae Scottocalanus Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Scolecitrichidae Scolecitrichopsis Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Scolecitrichidae Scolecithrix Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Scolecitrichidae Scolecithricella Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Scolecitrichidae Scaphocalanus Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Scolecitrichidae Racovitzanus Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Scolecitrichidae Pseudoamallothrix Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.

Key To The Species Of Scolecitrichidae Macandrewella Occurring In The China Seas, Chang-Tai Shih, Qing-Chao Chen, Yang-Chi Lan, Shih-Hui Hsiao, Chi-Yu Weng

Journal of Marine Science and Technology–Taiwan

No abstract provided.