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A Novel Mrna Delivery Strategy Employing Adenovirus Piggyback Mrna Binders Via Catcher/Tag Molecular Glue, Kexin Geng

McKelvey School of Engineering Graduate Student Theses & Dissertations

mRNA-based therapeutics open a new era for the prevention and treatment of a wide range of diseases. However, existing mRNA delivery vehicles have limited repertoires for dendritic cell targeting and intranasal administration which are two paramount delivery pathways of significant advantages for treating cancer and infectious respiratory diseases. Adenovirus (Ad) with well-deciphered viral genomes and intensive-studied structure biology has been developed for DCs-targeted cancer vaccines and intranasal COVID vaccines. In light of these virtues, Ad presents as the “off-the-shelf” tool possessing well-defined manufacturable capabilities and translatable feasibility that perfectly complements the current limitations of mRNA delivery. In this study, we …

The Biological Activity Of Dual-Responsive Glycopolymers For Intracellular Codelivery Of Antigen And Lipophilic Adjuvant, Micaela Shields

Honors Theses

Subunit vaccines present themselves as an attractive alternative to traditional approaches to vaccines, which utilizes whole organisms to trigger an immune response. While these traditional approaches have proven themselves reliable within the history of vaccinations, they generally deliver subpar robust cellular-mediated immunity and pose many safety risks. Composed of proteins and /or peptides, subunit vaccines are safer and more precise alternatives to traditional approaches. However, the pharmacokinetic properties of the macromolecules that make up these vaccines hinder the subunit vaccine’s level of immunogenicity [d1] Examples of this hindrance include but are not limited to aggregation at the injection site and …

Development Of Methods To Enhance Stem Cell Derived Islet Survival, Aining Fan

McKelvey School of Engineering Graduate Student Theses & Dissertations

No abstract provided.

Continuous Bioprocessing: Technology For Next-Generation Biopharmaceutical Manufacturing Development Of A Python-Coded Bench-Scale Raman-Based Continuous Bioprocess Platform, Christine Urrea

KGI Theses and Dissertations

Current industrial practices for producing biopharmaceuticals include fed-batch production with batch isolation and purification. Nonproductive hold-up steps and manual offline measurements are common in batch processing which increases processing time and contributes to a high cost of production. Current market trends and cost pressures in the biopharmaceutical industry are creating a push to innovate bioprocessing platforms. Continuous bioprocessing has been considered a solution to the current limitations of batch production of biopharmaceuticals. Continuous bioprocessing involves intensifying individual processing steps by eliminating hold-up steps through a continuous operation to increase productivity, which results in advantages such as lower capital and production …

The Development Of A Primer Payload With Microparticles For Uti Pathogen Identification Using Polythymidine- Modified Lamp Primers In Droplet Lamp, Jonas Otoo

KGI Theses and Dissertations

Nucleic acid amplification tests (NAATs) are among the diagnostic tests with the highest sensitivity and specificity. However, they are more complex to develop than other diagnostic tests such as biochemical tests and lateral flow immunoassay tests. Polymerase chain reaction (PCR) is the gold standard for NAATs. PCR requires thermal cycling to achieve clonal amplification of the target pathogen DNA for diagnosis. Thermal cycling poses a challenge in the development of PCR diagnostics for point-of-care (POC) settings. Loop-mediated isothermal amplification (LAMP) offers an isothermal method for NAATs diagnostics. The advancement of the microfluidics field significantly enhances the development of LAMP diagnostics …

Vi Energy-Efficient Memristor-Based Neuromorphic Computing Circuits And Systems For Radiation Detection Applications, Jorge Iván Canales Verdial

Electrical and Computer Engineering ETDs

Radionuclide spectroscopic sensor data is analyzed with minimal power consumption through the use of neuromorphic computing architectures. Memristor crossbars are harnessed as the computational substrate in this non-conventional computing platform and integrated with CMOS-based neurons to mimic the computational dynamics observed in the mammalian brain’s visual cortex. Functional prototypes using spiking sparse locally competitive approximations are presented. The architectures are evaluated for classification accuracy and energy efficiency. The proposed systems achieve a 90% true positive accuracy with a high-resolution detector and 86% with a low-resolution detector.

Transport Mechanisms In Electrochemical Immunoassay Biosensors With Applications To Sarscov-2 Neutralizing Antibody Detection, Mohammad Karim Dehghan Manshadi

Mechanical Engineering Research Theses and Dissertations

Biosensors are used in diverse applications spanning from clinical diagnosis and drug discovery to environmental and safety monitoring. Among different types of biosensors, electrochemical ones have demonstrated high sensitivity, short detection time, and selectivity with a low cost, and have been widely used to detect various diseases. In this thesis, we have introduced an inexpensive, rapid, sensitive, and quantifiable impedance-based immunosensors to evaluate SARS-CoV-2 neutralizing antibody (NAb), which shows the real protective immunity against COVID-19. We demonstrated that our device enabled assessment of NAb in a physiological buffer with conductivity equivalent to that of blood plasma. This technique can be …

Electromagnetically Actuated Modular Robots With Controlled Self-Assembly And On-Demand Disassembly, Anuruddha Bhattacharjee

Mechanical Engineering Research Theses and Dissertations

Modular reconfigurable robots, with on-demand assembly and disassembly, offer the possibility of mesoscale (milli- and microscale) manufacturing. However, most of the existing modular robotic platforms currently being designed and functionalized rely on complex actuation and assembly mechanisms and so lack further scalability and transformability required for mesoscale applications. Therefore, this dissertation seeks to develop a novel scalable modular robotic platform with controlled self-assembly, disassembly, and reconfiguration techniques using an external electromagnetic field. While current cutting-edge research in this area uses a single magnet to move one module at a time into the target position, this dissertation explores processes to move …

Photonic Sensors Based On Integrated Ring Resonators, Jaime Da Silva

Mechanical Engineering Research Theses and Dissertations

This thesis investigates the application of integrated ring resonators to different sensing applications. The sensors proposed here rely on the principle of optical whispering gallery mode (WGM) resonance shifts of the resonators. Three distinct sensing applications are investigated to demonstrate the concept: a photonic seismometer, an evanescent field sensor, and a zero-drift Doppler velocimeter. These concepts can be helpful in developing lightweight, compact, and highly sensitive sensors. Successful implementation of these sensors could potentially address sensing requirements for both space and Earth-bound applications. The feasibility of this class of sensors is assessed for seismic, proximity, and vibrational measurements.

Grammatical Triples Extraction For The Distant Reading Of Textual Corpora, Stephanie Buongiorno, Stephanie Buongiorno

Multidisciplinary Studies Theses and Dissertations

Grammatical triples extraction has become increasingly important for the analysis of large, textual corpora. By providing insight into the sentence-level linguistic features of a corpus, extracted triples have supported interpretations of some of the most relevant problems of our time. The growing importance of triples extraction for analyzing large corpora has put the quality of extracted triples under new scrutiny, however. Triples outputs are known to have large amounts of erroneous triples. The extraction of erroneous triples poses a risk for understanding a textual corpus because erroneous triples can be nonfactual and even analogous to misinformation. Disciplines such as the …

Antimicrobial Activity And Mechanism Of Amyloid Proteins And Synthetic Conjugated Polyelectrolytes, Fahimeh Maghsoodi

Nanoscience and Microsystems ETDs

Although the exact cause of Alzheimer’s disease (AD) is still unknown, it is widely considered that the accumulation of amyloid plaques composed of the amyloid-β (Aβ) peptide in the brain is linked to neurodegeneration. Co-localization of viral DNA with Aβ plaques, the association of brain infection and AD, and research indicating the protective effect of Aβ against bacteria and fungi in mice and human cells have led to the hypothesis that Aβ expression and deposition may be central to its function as an antimicrobial peptide (AMP). In my thesis research, we seek to elucidate how Aβ functions as an AMP …

Study Of A Carbon Fiber Reinforced Polymer Composite Using A Biobased Polyurethane As A Thermosetting Resin, Teddy Mageto

Electronic Theses & Dissertations

Carbon Fiber Reinforced Polymer composites (CFRP) have garnered increasing interest in recent years especially in the aerospace and automobile industries where they are gradually replacing metals as structural materials. This is owing to their light weight, high strength, high modulus, and excellent strength-to-weight ratio. Polymers are typically used as thermosetting resins in these composites. However, the synthesis of polymers currently is conducted via petrochemical processes which leads to adverse effects on the environment. To this end, in this work a biobased Polyurethane (PU) was used as a thermosetting resin in a CFRP. The biobased PU was synthesized by the reaction …

Synthesis And Characterization Of Polyaniline-Based High-Performance Carbon For Energy Storage Applications, Udeshika Kangane Arachchige

Electronic Theses & Dissertations

With the increase in global population, the demand for the energy required for daily activities including household necessities, electronic and electric devices, etc. enhances at an alarming proportion. Under these circumstances, supercapacitors (SCs) have attracted great attention as electrochemical energy storage devices. Nowadays, most commercially available supercapacitors are composed of metallic compounds. Due to the high consumption, and limited availability of these elements, it is necessary to find an alternative, effective, low-cost materials in place of using it. With the purpose of achieving high electrochemical performance, this research work focuses on developing carbon derived from polyaniline (PANI) as an efficient …

3d Printing Of Earthen Materials: Toward The Carbon-Zero Construction, Shiva Bhusal

Civil Engineering ETDs

In recent years, digital manufacturing techniques in the architecture and construction industry have rapidly gained attention, especially with 3D printing, also called additive manufacturing. The use of cement has been common in modern construction and is now being explored for 3D printing. However, due to cement's contribution to approximately 7 percent of global carbon emissions and its high cost, research on sustainable and greener materials has gained attention. Previous studies on alternative construction have examined the mechanical and rheological properties of soil mixes, but not much attention has been given to printability evaluation, shrinkage, and cracking, which can affect the …

Sensor Updates For Bigheaded Carp-Tracking Autonomous Boat, Jordan Kaufmann

Honors College Theses

Bigheaded carp are an invasive species that overpopulate and compete with the native species of Kentucky Lake as well as many other North American aquatic ecosystems. The movement patterns of Bigheaded carp are being studied nationwide by the United States Geological Survey and multiple universities. These studies ultimately seek to control their spread and reduce or reverse the ecosystem destabilization caused by this invasive species. Such studies are currently conducted manually on Kentucky Lake by graduate students affiliated with the Murray State University (MSU) Biology Department and Hancock Biological Station, and these manual studies are an arduous and time-consuming effort. …

How Students Investigate: Understanding Students’ Use Of Scientific Schemas In Open-Ended Labs, J. Alexander Wall

Masters of Education in Teaching and Learning

Schema provides a theoretical framework for understanding the nature of science, inquiry, and open-ended labs. These topics have been emphasized in the state standards, especially in high school sciences. In this study the researcher took field notes, collected lab write ups, and conducted focus group interviews to understand students’ use of schema related to open-ended labs. These data were analyzed with the constant comparative method to generate themes. The author describes the major findings with parallel narratives, one focused on a student’s confusion and the other focused on a student’s understanding. The author concludes by advocating for science teachers to …

Design Of University Small-Scale Dairy Processing Facility, Philip J. Hildebrand, Joshua Arejola, Alicia Andersen, Terry Howell Jr, Tami M. Brown-Brandl

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This project was assigned to research the feasibility and value of implementing a dairy processing facility on the campus of the University of Nebraska-Lincoln. The facility would process milk produced from cows under the same roof and will serve as an educational experience for Nebraska dairy farmers, UNL students, and K-12 students in the Lincoln-Lancaster County area. If the project is successful and replicated across the state, this facility could have a significant impact on the reduction of milk transportation costs in the Nebraska dairy industry.

The project began with researching milk processing methods and steps from production to consumption. …

Integrated Neutrosophic Best-Worst Method For Comprehensive Analysis And Ranking Of Flood Risks: A Case Study Approach From Aswan, Egypt, Safa Al-Saeed, Nabil M. Abdelaziz

Neutrosophic Systems with Applications

This paper presents a comprehensive framework for the analysis and ranking of flood risks with respect to regional variations and data uncertainties under a Neutrosophic environment. The research introduces a novel approach to flood risk mitigation and management, bringing together the scientifically robust Best-Worst Method (BWM) and single-valued Neutrosophic set for the first time. The unique application of a single-valued Neutrosophic set allows us to better illustrate and manage uncertainty, imprecision, and vagueness in data. Additionally, we employ BWM, a multi-factor decision-making method, for discerning and ranking the most influential flood risk factors. Together, the integrated methodologies provide a balanced, …

Understanding Vascular Calcification Through The Lens Of Canonical Wnt Signaling, Karlee Mcneel

Theses and Dissertations

Every 37 seconds, someone in the United States dies from cardiovascular disease. Vascular calcification is one of the underlying causes of these fatal events. Medial calcification develops following arteriosclerosis, or hardening of the arteries. Medial calcification is characterized by the deposition of hydroxyapatite in the medial layer of the arteries after normal vascular smooth muscle cells undergo a phenotypic switch to resemble osteoblast-like cells. It is hypothesized that this switch is caused by the wingless related (WNT)-Signaling pathway. The WNT-Signaling pathway, upon activation, causes the upregulation of osteogenic markers for the development of osteoblast-like cells. Current treatments alleviate consequences of …

An Investigation Into The Common Problems Of Lagoon Wastewater Treatment In Kentucky, Kaylee A. Jones

Honors Theses

Lagoon wastewater treatment systems are commonly used in areas that do not have the available resources for typical mechanical wastewater treatment. Lagoon systems consist of multiple preliminary lagoons, but mainly rely on long-term facultative lagoons for most analyte removal. In this study, one particular lagoon wastewater treatment system (named Municipality A Wastewater Treatment Plant) is investigated due to noncompliance with governmental standards provided by the National Pollutant Discharge Elimination System (NPDES). Samples were collected by researchers at 4 different instances inside of the treatment systems. These samples were sent to an analytical laboratory for testing. The readings from these samples …

An Investigation Into Perceptions, Expectations, And Development Of Professional Skills In Engineering Students, Morgan Kathryn Green

Theses and Dissertations

Upon graduation from an undergraduate institution, engineering graduates are expected to have a baselevel skill in technical skills related to their discipline. Teaching technical skills comes naturally to engineering programs as the conceptual understanding of the material form the foundation of engineering ability. However, engineering graduates also are expected to have a baselevel of professional skills, which are more subjective in nature and do not have a standardized approach for teaching or assessing them at the undergraduate level. An investigation into current perceptions of professional skills by relevant parties is an initial step in providing more structure to professional skills …

Where Did They Come From? Why Did They Go? How Engineering Students’ Perceptions Cultivate Experiences And Influence Behaviors, Sara Campbell Vick

Theses and Dissertations

Engineering undergraduate students have opinions and perceptions of engineering disciplines and engineering undergraduate students do not always matriculate and graduate in precisely the same discipline. Understanding how these two characteristics of engineering undergraduate students interact to inform behaviors is important for engineering educators and administrators to increase and improve recruitment and retention among their students.

This dissertation approached each characteristic of engineering students, first separately and then together. A nationwide survey of undergraduate engineering students found significant differences in how students perceive various engineering disciplines along several paired-term anchored scales. These differences were equally significant when scores were considered in …

Robotic Cotton Harvesting With A Multi-Finger End-Effector: Research, Design, Development, Testing, And Evaluation, Hussein Gharakhani

Theses and Dissertations

Cotton is harvested with large and heavy machines that are very efficient but have some disadvantages. They can harvest the crop only once at the end of the growing season. Since cotton bolls do not mature uniformly, the early opened bolls expose their fiber to weather for extended periods, reducing lint quality. In addition, the machines can also compact the soil, reducing water and fertilizer usage efficiencies and crop yields in the following years. Robotic cotton harvesting offers a promising solution to these issues. Smaller robotic harvesters could go to the field multiple times during the season to pick cotton …

Hydrological And Water Quality Assessment Of Forested Coastal Watersheds, Shreeya Bhattarai

Theses and Dissertations

Coastal regions are at risk of environmental threats. Flooding in coastal rivers is the result of intense precipitation which is triggered by climate change. Coastal watersheds are prone to losing significant amounts of sediment and nutrients because of the shorter transport pathway that drains directly into the coastal water. In this study, the hydrology, flood frequency, and water quality assessment of two coastal watersheds, Wolf River watershed (WRW) and Jourdan River watershed (JRW), were conducted using the Soil and Water Assessment Tool (SWAT). Since WRW and JRW are the main tributaries to fetch freshwater to Saint Louis Bay (SLB) of …

Effects Of Alternative Jet Fuels On Aerospace-Grade Composites: Experimental And Modeling Studies, Naoufal Harich

Theses and Dissertations

The aviation industry aims to reduce its environmental impact through innovation and research. The usage of composite materials for multiple primary structures represents one such measure. Several alternative fuels were approved and used along with the Federal Aviation Administration (FAA). These alternative fuels are produced from wastes and biomasses. Some alternative fuels were initially only approved as drop-in fuels, meaning they must be blended with conventional fuels to operate. Fuel tanks are usually embedded into the wing structure, which is mainly made of composite materials. These composites tend to absorb fluids it encounters through their matrix phase. The absorption behavior …

Evaluation Of Conservation Practices Effect On Water Quality Using The Swat Model, Vivek Venishetty

Theses and Dissertations

The deterioration of water quality due to human-driven alternations has an adverse effect on the environment. More than 50% of surveyed surface water bodies in the United States (US) are classified as impaired waters as per the Clean Water Act. The pollutants affecting the water quality in the US are classified as point and non-point sources. Pollutant mitigation strategies such as the selective implementation of best management practices (BMPs) based on the severity of the pollution could improve water quality by reducing the amounts of pollutants. Quantifying the efficiency of a specific management practice can be difficult for large watersheds. …

Managing Iron Deficiency Chlorosis (Idc) In Soybean Through A Cropping System Approach, Katelin Savannah Waldrep

Theses and Dissertations

Iron deficiency chlorosis (IDC) is a frequent problem throughout many areas of the United States where soils are high in calcium carbonate (CaCO₃), including the Blackland Prairie regions of Mississippi. The main objectives of this study were to 1) determine the effects of seven different cropping systems on IDC visual symptomology and grain yield in rainfed soybeans grown in calcareous soils, and 2) evaluate the effects of soil water tension (SWT) on IDC. Rotating soybeans with corn produced significantly higher yields for both tolerant and susceptible soybean varieties. IDC symptomology was worse, and yields were lower in cropping …

Risk Assessments And Modeling Of Driver By Using Risk Potential Theory, Riku Kikuta

Theses and Dissertations

Recently, various self-driving and driving assistance systems such as Advanced Driver Assistance System (ADAS) have been developed with the intent to reduce the number of motor vehicle accidents. While self-driving systems have been proven to reduce traffic accidents, the systems sometimes make other drivers confused because of their mechanical behavior. To avoid confusion and possible error, it is necessary to construct self-driving systems that exhibit human-like behaviors. Risk Potential theory has been used to construct models that successfully represent driver behavior, especially expert behavior. This project uses Risk Potential theory to construct and evaluate a collision avoidance driver model which …

Evaluation Of Early Maturing Cultivars, Optimal Harvest Timing, And Canopy Reflectance Of Peanut To Maximize Grade And Yield, Joseph Bryan Whittenton

Theses and Dissertations

Peanut digging timing is difficult to predict due to indeterminate growth and peanut pods maturing underground, resulting in the need to research methods that provide consistent measurements, while reducing time and effort for farmers and researchers. Experiments were conducted to evaluate the accuracy of the Maturity Index 1 and Maturity Index 2 in predicting peanut grade, the accuracy of the North Carolina 2 degree day method in predicting peanut yield, and remote sensing vegetative indices sensitivity equivalence (SEq) to peanut Maturity Index 2 and harvest grade (TSMK) for cultivars IPG-914 and Georgia-06G in Mississippi.

Maturity Index 1 and …

Wildfire-Altered Geotechnical Soil Properties And Their Impact On Subsequent Landslides, James M. Kellogg

Theses and Dissertations

Wildfires result in altered landscape as well as altered soil properties. Wildfires result in conditions that are more susceptible to geotechnical hazards introducing a potential for cascading events in the surrounding area. Shallow landslides are common geohazards that result in a post-wildfire landscape. With an ever-increasing trend in wildfires, the need to better understand the impact wildfire-altered soil properties play on shallow slope instability is critical to evaluating risks to existing or planned development, mitigation of potentially hazardous conditions, prioritization of post-wildfire planning, and post-wildfire response preparation. The primary objective of this study is to provide a comprehensive overview of …