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Resilin Distribution And Abundance In Apis Mellifera Across Biological Age Classes And Castes, Audrey Anderson, Noah Keime, Chandler Fong, Andrew Kraemer, Carol Fassbinder-Orth

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The presence of resilin, an elastomeric protein, in insect vein joints provides the flexible, passive deformations that are crucial to flapping flight. This study investigated the resilin gene expression and autofluorescence dynamics among Apis mellifera (honey bee) worker age classes and drone honey bees. Resilin gene expression was determined via ddPCR on whole honey bees and resilin autofluorescence was measured in the 1m-cu, 2m-cu, Cu-V, and Cu2-V joints on the forewing and the Cu-V joint of the hindwing. Resilin gene expression varied significantly with age, with resilin activity being highest in the pupae. Autofluorescence of the 1m-cu and the Cu-V …

The Role Of Leading-Edge Serrations In Controlling The Flow Over Owls’ Wing, Tanner Saussaman, Asif Nafi, David Charland, Hadar Ben-Gida, Roi Gurka

Physics and Engineering Science

We studied the effects of leading-edge serrations on the flow dynamics developed over an owl wing model. Owls are predatory birds. Most owl species are nocturnal, with some active during the day. The nocturnal ones feature stealth capabilities that are partially attributed to their wing microfeatures. One of these microfeatures is small rigid combs (i.e. serrations) aligned at an angle with respect to the incoming flow located at the wings' leading-edge region of the primaries. These serrations are essentially passive flow control devices that enhance some of the owls' flight characteristics, such as aeroacoustics and, potentially, aerodynamics. We performed a …

Stomatal Opening Efficiency Is Controlled By Cell Wall Organization In Arabidopsis Thaliana, Sedighe Keynia, Leila Jaafar, You Zhou, Charles T. Anderson, Joseph A. Turner

Department of Mechanical and Materials Engineering: Faculty Publications

Stomatal function in plants is regulated by the nanoscale architecture of the cell wall and turgor pressure, which together control stomatal pore size to facilitate gas exchange and photosynthesis. The mechanical properties of the cell wall and cell geometry are critical determinants of stomatal dynamics. However, the specific biomechanical functions of wall constituents, for example, cellulose and pectins, and their impact on the work required to open or close the stomatal pore are unclear. Here, we use nanoindentation in normal and lateral directions, computational modeling, and microscopic imaging of cells from the model plant Arabidopsis thaliana to investigate the precise …

Chitosan-Coated Lignin Nanoparticles Enhance Adsorption And Proliferation Of Alcanivorax Borkumensis At The Hexadecane-Water Interface, Amber J. Pete, Jin Gyun Lee, Michael G. Benton, Bhuvnesh Bharti

Chemical and Biochemical Engineering Faculty Research & Creative Works

Bioremediation of oil spills occurs when indigenous microbes metabolize petroleum hydrocarbons. Rapid proliferation of hydrocarbon-consuming bacteria near the oil-water interface is vital to optimizing the oil biodegradation rate. Adding nanoparticles (NPs) to microbial systems can enhance the bacterial growth rate. However, the role of NP source material and surface charge on their ability to impact bacterial growth and oil-water interface characteristics is poorly understood. Here, we investigate the role of NP composition and surface chemistry on the growth rate of hydrocarbon-consuming bacteria and its attachment to the oil-water interface. We use Alcanivorax borkumensis as a model organism because of its …

Water-Energy-Food Linkages In Community Smallholder Irrigation Schemes: Center Pivot Irrigation In Rwanda, Ankit Chandra, Derek M. Heeren, Lameck O. Odhiambo, N. Brozovic

Daugherty Water for Food Global Institute: Faculty Publications

Water, energy, and food are linked in intricate ways in irrigated agriculture and understanding the interplay of these components is crucial for sustainable and profitable crop production, particularly in smallholder setting such as in sub-Saharan Africa. This study evaluates water-energy-food linkages, engineering and economic performance, irrigation decision making, and challenges faced around water management in a community-based mechanized irrigation scheme in Rwanda. The research is the first to analyze such as scheme, which uses technology typically used by large farmers in a smallholder setting. The study investigates the variation in water requirements and the relationship and impacts of this variability …

Reducing Brain Kynurenic Acid Synthesis Precludes Kynurenine-Induced Sleep Disturbances, Katherine M. Rentschler, Snezana Milosavljevic, Annalisa M. Baratta, Courtney J. Wright, Maria V. Piroli, Zachary Tentor, Homayoun Valafar, Christian O'Reilly, Ana Pocivavsek

Publications

Patients with neurocognitive disorders often battle sleep disturbances. Kynurenic acid is a tryptophan metabolite of the kynurenine pathway implicated in the pathology of these illnesses. Modest increases in kynurenic acid, an antagonist at glutamatergic and cholinergic receptors, result in cognitive impairments and sleep dysfunction. We explored the hypothesis that inhibition of the kynurenic acid synthesising enzyme, kynurenine aminotransferase II, may alleviate sleep disturbances. At the start of the light phase, adult male and female Wistar rats received systemic injections of either: (i) vehicle; (ii) kynurenine (100 mg kg−1; i.p.); (iii) the kynurenine aminotransferase II inhibitor, PF-04859989 (30 mg kg−1; s.c.); …

Eipeck: Assessing Educators’ Pedagogical Content Knowledge For Engineering Integration In K-12, Pilar Pazos, Francisco Cima Cohuo, Jennifer Kidd, Kristie Gutierrez, Krishnanand Kaipa, Orlando Ayala

Journal of Pre-College Engineering Education Research (J-PEER)

Global efforts are underway to include engineering in pre-college curricula. In the USA, this pursuit led to the inclusion of engineering content in the most recent version of the Next Generation Science Standards that guide K-12 science. As these standards become part of the K-12 curriculum, teachers face the challenge of gaining basic engineering literacy, while developing the associated inclusive pedagogies necessary to integrate engineering content into their classrooms. In this context, teacher preparation programs can benefit from easy-to-implement tools that measure preservice teachers’ readiness to integrate engineering content in their future classrooms. This work describes the development and validation …

Analysis Of Factors Affecting Construction And Demolition Waste Reduction In Egypt, Ayman Ahmed Ezzat Othman, Ahmed Osama Daoud, Obas John Ebohon, Ali Bayyati

Architectural Engineering

Construction projects are associated with construction and demolition waste (CDW) generated at different stages. In Egypt’s case, the CDW problem has become a significant challenge, and the need to find sustainable solutions is overwhelming. Based on recent investigations in the literature, it was found that six main factors are affecting CDW reduction (CDWR) as follows: (1) waste-efficient materials procurement measures; (2) waste-efficient materials procurement models; (3) green materials procurement approach; (4) legislation; (5) culture & behaviour; and (6) awareness. In this study, a representative sample of Egyptian construction firms was screened to (1) investigate the applicability and effectiveness of CDWR …

Integrating Glycolysis, Citric Acid Cycle, Pentose Phosphate Pathway, And Fatty Acid Beta‑Oxidation Into A Single Computational Model, Sylwester M. Kloska, Krzysztof Pałczyński, Tomasz Marciniak, Tomasz Talaśka, Beata J. Wysocki, Paul H. Davis, Tadeusz A. Wysocki

Department of Electrical and Computer Engineering: Faculty Publications

The metabolic network of a living cell is highly intricate and involves complex interactions between various pathways. In this study, we propose a computational model that integrates glycolysis, the pentose phosphate pathway (PPP), the fatty acids beta-oxidation, and the tricarboxylic acid cycle (TCA cycle) using queueing theory. The model utilizes literature data on metabolite concentrations and enzyme kinetic constants to calculate the probabilities of individual reactions occurring on a microscopic scale, which can be viewed as the reaction rates on a macroscopic scale. However, it should be noted that the model has some limitations, including not accounting for all the …

Comparative Investigations On Microextraction And Conventional Air Sampling Techniques: Challenges And Future Directions, Firoz Ahmed, Mehedi Hasan Roni, Ashiqur Rahman, Sayed M A Salam

Al-Bahir

Microextraction technique (e.g., solid phase microextraction, thin film microextraction, in-tube extraction) brings a revolutionary change in air sampling techniques over the recent few years. This advanced technique exhibits a high pollutant extraction rate, a low retention time, and a lower error margin compared to conventional air sampling techniques. The accuracy range of microextraction technique (MET) was recorded ~90-95% to isolate the volatile organic components, oxygenated and halogenated carbon particles from the air. However, the efficiency of MET increases additional >3-5% when employed by coupled with gas chromatography or gas chromatography-mass spectrometry. The conventional sampling techniques (e.g., bag sampling, grab sampling) …

Neutrosophic Adaptive Lsb And Deep Learning Hybrid Framework For Ecg Signal Classification, Abdallah Rezk, Ahmed S. Sakr, H. M. Abdulkader

Applied Mathematics & Information Sciences

This paper proposes a novel hybrid framework for ECG signal classification and privacy preservation. The framework includes two phases: the first phase uses LSTM+CNN with attention gate for ECG classification, while the second phase utilizes adaptive least signal bit with neutrosophic for hiding important data during transmission. The proposed framework converts data into three sets of degrees (true, false, and intermediate) using neutrosophic and passes them to an embedding layer. In the sender part, the framework hides important data in ECG signal as true and false degrees, using the intermediate set as a shared dynamic key between sender and receiver. …

Compatibility Of Clique Clustering Algorithm With Dimensionality Reduction, Ug ̆Ur Madran, Duygu Soyog ̆Lu

Applied Mathematics & Information Sciences

In our previous work, we introduced a clustering algorithm based on clique formation. Cliques, the obtained clusters, are constructed by choosing the most dense complete subgraphs by using similarity values between instances. The clique algorithm successfully reduces the number of instances in a data set without substantially changing the accuracy rate. In this current work, we focused on reducing the number of features. For this purpose, the effect of the clique clustering algorithm on dimensionality reduction has been analyzed. We propose a novel algorithm for support vector machine classification by combining these two techniques and applying different strategies by differentiating …

The Effect Of System Quality And User Quality Of Information Technology On Internal Audit Effectiveness In Jordan, And The Moderating Effect Of Management Support, Ahmad Yahiya Ahmad Bani Ahmad (Ayassrah), Anas Ahmad Mahmoud Bani Atta, Hanan Ali Alawawdeh, Nawaf Abdallah Aljundi, Amer Morshed, Saleh Amin Dahbour

Applied Mathematics & Information Sciences

The goal of this study is to ascertain the moderating role that management support has in internal audit effectiveness in Jordan, as well as the impact of system quality and user quality of information technology. There were 172 responders in all, and they were split across Jordanian auditors. In the data analysis process, the quantitative analysis test— which consists of the validity test, reliability test, test of conventional assumptions, and hypothesis test—is applied. Information technology system and user quality are independent variables in this study. The dependent variable in this study is internal audit effectiveness, and the moderating variable is …

Optimal Control Analysis Of The Dynamics Of Covid-19 With Application To Ethiopian Data, Temesgen Duresa Keno, Fekadu Mosisa Legesse, Ebisa Olana Bajira

Applied Mathematics & Information Sciences

In this paper, we proposed an optimal control of the COVID-19 transmission dynamics. First, we investigated system features such as solution boundedness, positivity, disease-free and endemic equilibrium, and the local and global stability of equilibrium points. Besides, a disease-free equilibrium point is globally asymptotically stable if the basic reproduction number is less than one, and an endemic equilibrium point exists otherwise. Secondly, we have shown the sensitivity analysis of the basic reproduction number. Also the model is then fitted using COVID-19 infected reported in Ethiopia from February 1,2023 to March 2,2023. The values of model parameters are then estimated from …

Applications Of The Ara-Residual Power Series Technique To Physical Phenomena, Aliaa Burqan

Applied Mathematics & Information Sciences

In this paper, a new analytical method called the ARA-Residual power series method (ARA- RPSM) is implemented to solve some fractional physical equations. The methodology of the proposed method based on applying the ARA-transform to the given fractional differential equations, followed by the creation of approximate series solutions using Taylor’s expansion. Then the series solution is transformed using the inverse of the ARA-transform to get the solution in the original space. Accuracy, effectiveness, and validity of the suggested method are demonstrated through the discussion of three attractive applications. The solution obtained using ARA-RPSM demonstrates good agreement when compared to the …

Generalization Of Renyi’S Entropy And Its Application In Source Coding, Ashiq Hussain Bhat, Niyamat Ali Siddiqui, Ismail A Mageed, Shawkat Alkhazaleh, Vidyanand Rabi Das, M. A. K Baig

Applied Mathematics & Information Sciences

In this paper, we introduce a new generalization of Renyis entropy β(P) and the most important feature of this generalized entropy Rαβ (P) is that it derives most important entropies that are well known and influence information theory and applied mathematics. Some significant properties of Rαβ (P) has been undertaken in this article. In addition, we introduce a new generalized exponentiated mean codeword length Lβα (P) in this article then determine how Rβα (P) and Lβα (P) are related in terms of source coding theorem.

Shrinkage And Consolidation Characteristics Of Chitosan-Amended Soft Soil: A Sustainable Alternate Landfill Liner Material, Romana Mariyam Rasheed, Arif Ali Baig Moghal, Sai Sampreeth Reddy Jannepally, Ateekh Ur Rehman, Bhaskar C. S. Chittoori

Civil Engineering Faculty Publications and Presentations

Kuttanad is a region that lies in the southwest part of Kerala, India, and possesses soft soil, which imposes constraints on many civil engineering applications owing to low shear strength and high compressibility. Chemical stabilizers such as cement and lime have been extensively utilized in the past to address compressibility issues. However, future civilizations will be extremely dependent on the development of sustainable materials and practices such as the use of bio-enzymes, calcite precipitation methods, and biological materials as a result of escalating environmental concerns due to carbon emissions of conventional stabilizers. One such alternative is the utilization of biopolymers. …

Construction And Performance Optimization Of Bioconjugated Nanosensors For Early Detection Of Breast Cancer And Pro-Inflammatory Diseases, Pooja Gaikwad

Dissertations, Theses, and Capstone Projects

In recent years, nanosensors have emerged as a tool with strong potential in medical diagnostics. Single-walled carbon nanotube (SWCNT) based optical nanosensors have notably garnered interest due to the unique characteristics of their near-infrared fluorescence emission, including tissue transparency, photostability, and various chiralities with discrete absorption and fluorescence emission bands. Additionally, the optoelectronic properties of SWCNT are sensitive to the surrounding environment, which makes them suitable for in vitro and in vivo biosensing. Single-stranded (ss) DNA-wrapped SWCNTs have been reported as optical nanosensors for cancers and metabolic diseases. Breast cancer and cardiovascular diseases are the most common causes of death …

Quantifying Temperature-, Pressure-, And Nuclear Quantum Effects On Hydrophobic And Hydrophilic Water-Mediated Interactions, Justin T. Engstler

Dissertations, Theses, and Capstone Projects

Water-mediated interactions (WMIs) are responsible for diverse processes in aqueous solutions, including protein folding and nanoparticle aggregation. WMI may be affected by changes in temperature and pressure, and hence, they can alter chemical/physical processes that occur in aqueous environments. Traditionally, attention has been focused on hydrophobic interactions while, in comparison, the role of hydrophilic and hybrid (hydrophobic–hydrophilic) interactions have been mostly overlooked. Here, we study the role of T and P on the WMI between nanoscale (i) hydrophobic–hydrophobic, (ii) hydrophilic–hydrophilic, and (iii) hydrophilic–hydrophobic pairs of (hydroxylated/non-hydroxylated) graphene-based surfaces. We find that hydrophobic, hydrophilic, and hybrid interactions are all sensitive to …

Tunable Linear And Nonlinear Metasurfaces Based On Hybrid Gold-Graphene Plasmons, Matthew Feinstein

Dissertations, Theses, and Capstone Projects

Optical Metasurfaces are planar structures that are patterned with subwavelength structures and are very thin compared to the wavelength of light. Despite their thinness, these structured materials can strongly interact with incident light to effect the functionalities of conventional optical components, such as rotation of the polarization state, beam steering, lensing, spectral filtering, and holography, to name a few. Metasurfaces can also facilitate nonlinear optical effects, such as the mixing of beams at different frequencies to generate a beam at a new frequency.

The ability to alter the behavior of a metasurface during operation is highly desired for applications such …

Qc-Sane: Robust Control In Drl Using Quantile Critic With Spiking Actor And Normalized Ensemble, Surbhi Gupta, Gaurav Singal, Deepak Garg, Sarangapani Jagannathan

Electrical and Computer Engineering Faculty Research & Creative Works

Recently Introduced Deep Reinforcement Learning (DRL) Techniques in Discrete-Time Have Resulted in Significant Advances in Online Games, Robotics, and So On. Inspired from Recent Developments, We Have Proposed an Approach Referred to as Quantile Critic with Spiking Actor and Normalized Ensemble (QC-SANE) for Continuous Control Problems, Which Uses Quantile Loss to Train Critic and a Spiking Neural Network (NN) to Train an Ensemble of Actors. the NN Does an Internal Normalization using a Scaled Exponential Linear Unit (SELU) Activation Function and Ensures Robustness. the Empirical Study on Multijoint Dynamics with Contact (MuJoCo)-Based Environments Shows Improved Training and Test Results Than …

Multifunctional Organic Monolayer-Based Coatings For Implantable Biosensors And Bioelectronic Devices: Review And Perspectives, Taral Patel, Jie Huang, Katarzyna Krukiewicz

Electrical and Computer Engineering Faculty Research & Creative Works

The Emerging Field of Biosensors and Bioelectronics Seeks to Exploit Biology in Combination with the Recent Advances in Engineering. Even Though Biomedical Implants Can Significantly Improve Quality of Life, Problems Following the Process of Implantation Are Still an Issue. in This Review, We Point Out Organic Monolayer-Based Coatings as a Potential Solution to the Major Limitations of Implantable Biomaterials, Including Limited Biocompatibility, the Risk of Biofouling, Bacterial Colonization, and Stability under in Vivo Conditions. at First, Selected Current and Perspective Biomaterials Are Discussed, with the Focus on their Use as Implantable Biosensors and Biomedical Electrodes. Surface Modification Strategies for Implantable …

Impact Of Corrosion Inhibitors On Antibiotic Resistance, Metal Resistance, And Microbial Communities In Drinking Water, Lee K. Kimbell, Emily Lou Lamartina, Stan Kohls, Yin Wang, Ryan J. Newton, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Corrosion inhibitors, including zinc orthophosphate, sodium orthophosphate, and sodium silicate, are commonly used to prevent the corrosion of drinking water infrastructure. Metals such as zinc are known stressors for antibiotic resistance selection, and phosphates can increase microbial growth in drinking water distribution systems (DWDS). Yet, the influence of corrosion inhibitor type on antimicrobial resistance in DWDS is unknown. Here, we show that sodium silicates can decrease antibiotic resistant bacteria (ARB) and antibiotic-resistance genes (ARGs), while zinc orthophosphate increases ARB and ARGs in source water microbial communities. Based on controlled bench-scale studies, zinc orthophosphate addition significantly increased the abundance of ARB …

Guar-Based Injectable Hydrogel For Drug Delivery And In Vitro Bone Cell Growth, Humandra Poudel, Ambar R. Rangumagar, Pooja Singh, Adeolu Oluremi, Nawab Ali, Fumiya Watanabe, Joseph Batta-Mpouma, Jin-Woo Kim, Ahona Ghosh, Anindya Ghosh

Chemistry & Biochemistry Faculty Publications and Presentations

Injectable hydrogels offer numerous advantages in various areas, which include tissue engineering and drug delivery because of their unique properties such as tunability, excellent carrier properties, and biocompatibility. These hydrogels can be administered with minimal invasiveness. In this study, we synthesized an injectable hydrogel by rehydrating lyophilized mixtures of guar adamantane (Guar-ADI) and poly-β-cyclodextrin (p-βCD) in a solution of phosphate-buffered saline (PBS) maintained at pH 7.4. The hydrogel was formed via host-guest interaction between modified guar (Guar-ADI), obtained by reacting guar gum with 1-adamantyl isocyanate (ADI) and p-βCD. Comprehensive characterization of all synthesized materials, including the hydrogel, was performed using …

Degredation Of Organic Pollutants In Flocculated Liquid Digestate Using Photocatalytic Titanate Nanofibers: Mechanism And Response Surface Optimization, Yiting Xiao, Yang Tian, Yuanhang Zhan, Jun Zhu

Biological and Agricultural Engineering Faculty Publications and Presentations

Titanate nanofibers (TNFs) were synthesized using a hydrothermal method and were employed for the first time in this study to photocatalytically degrade organic pollutants found in flocculated liquid digestate of poultry litter. The photocatalytic performance of TNFs, with a bandgap of 3.16 eV, was tested based on degradation of organic pollutants and removal of color. Five combinations of pollutant concentration and pH were examined (0.2 to 1.3 g·L⁻¹ at pH 4 to 10). Central composite design (CCD) and response surface methodology (RSM) were applied in order to optimize the removal rates of volatile fatty acids (VFA) and chemical oxygen …

Alkaline Pretreatment And Air Mixing For Improvement Of Methane Production From Anaerobic Co-Digestion Of Poultry Litter With Wheat Straw, Yuanhang Zhan, Jun Zhu, Yiting Xiao, Leland C. Schrader

Biological and Agricultural Engineering Faculty Publications and Presentations

Alkaline pretreatment (AL) and air mixing (air) both have the potential to improve anaerobic co-digestion (Co-AD) of poultry litter with wheat straw for methane production. In this study, the effects of the combination of AL (pH 12 for 12 h) and air mixing (12 mL·d⁻¹) on the Co-AD process were investigated. The substrate hydrolysis was enhanced by AL, with soluble chemical oxygen demand increased by 4.59 times and volatile fatty acids increased by 5.04 times. The cumulative methane yield in the group of Co-AD by AL integrated with air (Co-(AL + air)), being 287 mL·(g VS_added) …

Non-Planar 3d Printing Of Thermosetting Prepolymers, Junaid Abid Ali

Master's Theses

Additive manufacturing or three-dimensional (3D) printing is an advanced process of manufacturing that challenges traditional manufacturing methodologies. However, the current 3D printing process does exhibit specific limitations. It generates printing paths that are in a horizontal plane, proving inadequate for printing curved or non-flat structures. This study established a printing procedure capable of simultaneously executing printing in X, Y, and Z directions. The limitations posed by conventional 3D printing lead to the poor surface quality of curved printed objects. In contrast, non-planar 3D printing provides smoother surfaces. This work developed a program capable of producing non-planar print paths for the …

Antibiotic Resistance In Urban Stormwater: A Review Of The Dissemination Of Resistance Elements, Their Impact, And Management Opportunities, Kassidy N. O'Malley, Walter M. Mcdonald, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

The public health crisis of antibiotic resistance is a growing threat across the world that is only expected to intensify in the coming years. The cycling of antibiotic resistance genes (ARGs) in the environment via urban stormwater runoff is one means by which humans are exposed to resistant bacteria as traditional gray stormwater infrastructure facilitates the transport of resistance elements into water bodies utilized by the public. In this review, existing research on the occurrence of ARGs in urban stormwater runoff is critically reviewed with the goal of determining the role of stormwater in the dissemination and development of antibiotic …

Uncertainties In Retrieval Of Remote Sensing Reflectance From Ocean Color Satellite Observations, Eder I. Herrera Estrella

Dissertations, Theses, and Capstone Projects

Ocean Color radiometry uses remote sensing to interpret ocean dynamics by retrieving remote sensing reflectance (������) from satellite imagery at different scales and over different time periods. ������ spectrum characterizes the ocean color that we observe, and from which we can discern concentrations of chlorophyll, organic and inorganic particles, and carbon fluxes in the ocean and atmosphere. ������ is derived from the total radiance at the top of the atmosphere (TOA). However, it only represents up to ten percent of the total signal. Hence, the retrieval of ������ from the total radiance at TOA involves the application of atmospheric correction …

Syne1 Mutation Is Associated With Increased Tumor Mutation Burden And Immune Cell Infiltration In Ovarian Cancer, Laura M. Harbin, Nan Lin, Frederick R. Ueland, Jill M. Kolesar

Markey Cancer Center Faculty Publications

SYNE1, a nuclear envelope protein critical for cellular structure and signaling, is downregulated in numerous malignancies. SYNE1 alterations are found in 10% of gynecologic malignancies and 5% of epithelial ovarian cancers. Previous studies demonstrated an association between SYNE1 mutation, increased tumor mutation burden (TMB), and immunotherapy response. This study evaluates the SYNE1 mutation frequency, association with TMB, and downstream effects of SYNE1 mutation in ovarian cancer. Genetic information, including whole-exome sequencing, RNA analysis, and somatic tumor testing, was obtained for consenting ovarian cancer patients at an academic medical center. Mutation frequencies were compared between the institutional cohort and The Cancer …