Digital Commons Network^™

Investigating A Passive Treatment System For The Removal Of Nutrients From Urban Runoff, Jasminn Gray

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Environmental Protection Agency recognizes urban runoff as a major contributor of surface water pollution with nutrients as the second largest cause of surface water impairment in the United States. While a water quality standard of 100 lbs/day total phosphorus load allocation for all nonpoint sources is permitted for the Las Vegas Valley (LVV) under the National Pollutant Discharge Elimination System, currently there is not a counterpart standard for nitrogen. With the continued development of the LVV and the depletion of the quantity of water in Lake Mead due to the ongoing drought, the concentrations of phosphorus and nitrogen species …

Virus Removal And Characterization During Secondary Treatment At A Full-Scale Plant, Tyler Hill

UNLV Theses, Dissertations, Professional Papers, and Capstones

Virus removal is paramount to water reuse applications and nearly all water reuse applications require extensive pathogen reduction because of high influent concentration, difficulty of detection and distinguishing infectivity. Typically, regulations stipulate virus attenuation that require the implementation of multi-barrier advanced treatment trains and award these reductions in log notation called log reduction values (LRVs). Reduction of virus concentrations are not awarded for virus removal that may occur in wastewater treatment steps which precede water reuse units such as activated sludge secondary biological treatment.

A systematic literature review was performed and virus reduction at full-scale wastewater treatment plan was monitored …

Engineered Biomass-Derived Carbon Adsorbents For Targeted Contaminant Remediation From Aqueous Environments, Suraj Venkat Pochampally

UNLV Theses, Dissertations, Professional Papers, and Capstones

Porous carbon adsorbents like biochar (BC) are produced from waste biomasses like wood chips, animal litter, nut shells, dried grass, etc. to be used in a multitude of applications like gas capture, soil amendment, water treatment, electrode materials, etc. In water treatment, carbon-based adsorbents have been mainly used to physically or chemically adsorb contaminants depending on the adsorption mechanism. However, BCs in their raw state do not have the capability of absorbing high amounts of contaminants and need to be activated/ modified for the pore network to broaden and adsorb the contaminants with better efficiency.

The scope of this dissertation …

Treatment Of Oxyanion Contaminants From Waste Brine Streams Using Halophilic/Halotolerant Microorganisms, Yasaman Saedi

UNLV Theses, Dissertations, Professional Papers, and Capstones

Brine waste streams are a current environmental issue given their high concentrations of contaminants and total dissolved solids (TDS), specifically sodium and chloride. Developing technologies that address brine treatment challenges is an essential need for water and wastewater industries. In addition to high salinity industrial wastes, there are several water treatment technologies producing brine waste streams in high volumes including reverse osmosis (RO), membranes, and Ion-exchange. With rising water demands and decreasing water supply, RO membranes will be extensively employed for potable water reuse and water desalination. Reusable ion exchange resins are also widely used to remove oxyanions contaminants from …

Rabrs For Use In Biologically Enhanced Precipitation Of Struvite In Anaerobic Digester Effluent (Municipal Wastewater), Parker R. Goldsberry

All Graduate Theses and Dissertations, Fall 2023 to Present

Rotating Algae Biofilm Reactor (RABR) technology has been researched in studies over the past decade directed at nutrient management for water resource recovery facilities (WRRFs). This study investigated the growth of slow-release fertilizer crystals, referred to as struvite, in the algae biofilms of Rotating Algae Biofilm Reactors (RABRs) at the Central Valley Water Reclamation Facility, the largest water resource recovery facility in the State of Utah. RABRs used anaerobic digester (AD) filtrate as their nutrient source. AD effluent is high in nutrients like nitrogen and phosphorus. The levels of both phosphorus and nitrogen (in the form of ammonia) are regulated …

Surface Water Hydrology Within The Great Salt Lake Basin, Madeline F. Merck

All Graduate Theses and Dissertations, Fall 2023 to Present

The Great Salt Lake (GSL) and its tributaries are ecologically and economically important resources for Utah. It is a highly saline lake, with salinity that is several times saltier than the ocean. Lake uses are heavily influenced by its salinity and lake level and, similarly, river uses by its streamflow quantity. Currently, the GSL Basin is experiencing changes that affect tributary inflows, lake level, and salinity. For my dissertation and in support of better lake management, I addressed important gaps in our understanding of the lake and the changes it is undergoing.

Chapter 2 is an examination of the distribution …

Effects Of Indole Derivative Idc16 On Cell Proliferation, Transfection, And Alternative Splicing In Production Of Lentiviral Vectors By Transient Co-Transfection, Nataly Mier

All Graduate Theses and Dissertations, Fall 2023 to Present

Viruses can efficiently introduce genetic material into a variety of cells through cell infection. Therefore, viral vectors, defined as virus-based gene delivery systems, effectively transfer functional gene constructs to target cells. Among different viral vectors, lentiviral vectors are of interest due to the advantages offered to research and therapeutic applications. Low productivity of lentiviral vectors in mammalian producer cells and high production cost, however, remain barriers to the vectors’ routinary use, particularly in clinical settings.

Since most lentiviral vectors are derived from human immunodeficiency virus type I, their genome has been engineered to increase safety. Nevertheless, modifications of the lentiviral …

Comparing Scaling Approaches To Estimate Unimpaired Streamflow Timeseries And Seasonal Flow Metrics At Ungauged Streams, Karl Christensen

All Graduate Theses and Dissertations, Fall 2023 to Present

River ecosystems are controlled in part by natural variation in the flow regime and streamflow alterations that impair these natural variations often have negative impacts for aquatic species. Flow metrics describing attributes of the natural flow regime can inform environmental water management goals to maintain and restore river ecosystems by mirroring critical aspects of the natural flow regime. However, unimpaired daily streamflow data needed to calculate these flow metrics is not always readily available. Statistical scaling approaches present an opportunity to estimate unimpaired flow metrics at ungauged locations to better address environmental water management objectives. This study evaluates a suite …

Understanding The Effects Of A Mobile Flow Visualization Learning Tool On Engineering Undergraduate Interest In Fluid Mechanics, Lori M. Openshaw

All Graduate Theses and Dissertations, Fall 2023 to Present

Fluid mechanics is a difficult engineering course that tends to act as a weed-out course for undergraduate students. Despite this, fluid mechanics is critical, with related careers in disciplines such as Mechanical, Aerospace, Civil, Environmental, Biological, Ocean, and Naval engineering. Within these fields, there is a lack of graduates with sufficient fluid mechanics knowledge to fill the available positions. One solution to this career pipeline is to increase student interest and understanding of fluid mechanics.

Efforts to make fluid mechanics more interesting and easily understood include curricular changes such as emphasizing historical aspects of fluids, computer simulations that model complex …

Extremum Seeking Control Algorithms For Extremely High Frequency Antenna System Pointing, Scott Shore

Mechanical Engineering ETDs

The research examines the application of extremum seeking control (ESC) algorithms to ground station antenna pointing for extremely high frequency (EHF) communication systems. ESC algorithms search for local maxima or minima by locating where an objective function gradient goes to zero. With wireless communication expanding into higher frequencies, the ground station pointing requirement is increasing. ESC presents a method which utilizes available equipment and information to perform ground station pointing. Additionally, ESC algorithms do not rely on assumptions or approximations needed for other techniques. The dissertation demonstrates ESC algorithm feasibility for the ground station pointing problem, benchmarks the ESC algorithms …

Nutritional-Environmental Trade-Offs In Potato Storage And Processing For A Sustainable Healthy Diet, Aubin Payne, Ebenezer M. Kwofie, Prince Agyemang, Jamie Baum

Biological and Agricultural Engineering Faculty Publications and Presentations

Over the last decade, poor diets and limited access to nutritious foods have been critical drivers of micronutrient deficiency in human health. However, food fortification at an industrialized scale in developed countries has helped eliminate deficiency-related diseases. In developing countries, fortified foods and biofortified materials have been delivered to nutrient-deficient communities. While these strategies have produced significant and acclaimed results, reports from the Food and Agricultural Organization suggest that over a quarter of the world’s population suffers from micronutrient deficiency. This implies that there are still declines in micronutrients in food products at different nodes along the food value chain …

Representativeness Evaluation Of Delta-Flux Eddy Covariance Towers For Assessing Greenhouse Gas Emissions From Rice Fields In The Mid-South Region, Katherine Skiles

Biological and Agricultural Engineering Undergraduate Honors Theses

Rice grown in 5 states across the Mid-South (AR, LA, MS, TX, and MO) account for nearly 80% of all domestic U.S. rice production. Methane emissions produced during rice farming and the large seasonal water demand to maintain flooded fields have created a need for alternative irrigation research that can reduce methane emissions and conserve water. Structures called eddy covariance towers (ECTs) can observe methane and other flux responses to changing irrigation styles, and their data is shared across flux tower networks such as Delta-Flux in the Mid-South. The Delta-Flux network is relatively new, and its data-sharing capacity is not …

Examining The Benefits Of Using Rainfall To Supplement Alternate Wetting And Drying Irrigation In Zero-Grade Rice Fields In Central Arkansas, Leo Black

Biological and Agricultural Engineering Undergraduate Honors Theses

Arkansas farmers account for more than 50% of United States production of rice, a crop that requires 3 to 5 times as much water as other regional crops. Methods of reducing water use and increasing its efficiency are being explored throughout the rice production industry, including irrigation management, better land grading, and precipitation capture. This project investigates the combination of these methods. The objectives of this project were to assess field-level water inputs, identify relationships between rice growth and water input, and analyze weather patterns in zero-grade fields in central Arkansas. Subsequent results were used to determine if there is …

Controlled Manipulation And Transport By Microswimmers In Stokes Flows, Jake Buzhardt

All Dissertations

Remotely actuated microscale swimming robots have the potential to revolutionize many aspects of biomedicine. However, for the longterm goals of this field of research to be achievable, it is necessary to develop modelling, simulation, and control strategies which effectively and efficiently account for not only the motion of individual swimmers, but also the complex interactions of such swimmers with their environment including other nearby swimmers, boundaries, other cargo and passive particles, and the fluid medium itself. The aim of this thesis is to study these problems in simulation from the perspective of controls and dynamical systems, with a particular focus …

Deficiency Of Acute-Phase Serum Amyloid A Exacerbates Sepsis-Induced Mortality And Lung Injury In Mice, Ailing Ji, Andrea C. Trumbauer, Victoria P. Noffsinger, Luke W. Meredith, Brittany Dong, Qian Wang, Ling Guo, Xiangan Li, Frederick C. De Beer, Nancy R. Webb, Lisa R. Tannock, Marlene E. Starr, Christopher M. Waters, Preetha Shridas

Saha Cardiovascular Research Center Faculty Publications

Serum amyloid A (SAA) is a family of proteins, the plasma levels of which may increase >1000-fold in acute inflammatory states. We investigated the role of SAA in sepsis using mice deficient in all three acute-phase SAA isoforms (SAA-TKO). SAA deficiency significantly increased mortality rates in the three experimental sepsis mouse models: cecal ligation and puncture (CLP), cecal slurry (CS) injection, and lipopolysaccharide (LPS) treatments. SAA-TKO mice had exacerbated lung pathology compared to wild-type (WT) mice after CLP. A bulk RNA sequencing performed on lung tissues excised 24 h after CLP indicated significant enrichment in the expression of genes associated …

Advancing Additive Manufacturing For Biomedical Applications: Antimicrobial And X-Ray Absorptive Composite Filaments For Fused Filament Fabrication, John M. Arnold Jr

LSU New Orleans Theses and Dissertations

The objective of this research was to investigate the use of nano- and microparticle amendments for the creation of Poly-lactic Acid composite materials for use in biomedical applications using the Fused Filament Fabrication process of Additive Manufacturing. Composites were created with the goal of imparting the useful properties of antimicrobial activity and x-ray absorption to the material. In addition to testing the efficacy of the particle amendments in achieving the desired properties, the thermal and mechanical properties of the composite materials were tested to ensure that the composites would be compatible with the Fused Filament Fabrication process and would produce …

A Study Of Group Effectiveness In A Virtual Reality Environment., Dalton Clay Sparks

Electronic Theses and Dissertations

Virtual Reality (VR) technology has revolutionized the study of team effectiveness, yet many studies in this domain lack a comprehensive approach, focusing narrowly on performance metrics. This thesis investigates VR team effectiveness by integrating traditional Team Effectiveness frameworks with VR-specific dynamics. Utilizing Data Envelopment Analysis (DEA) and Group Style Inventory (GSI), this study assesses teams within a VR environment, specifically a multiplayer submarine simulation game, IronWolfVR. Fifteen groups, totaling 45 participants, were observed for their collaborative gameplay, incorporating physiological measures (Heart Rate and Electrodermal Activity), team coordination dynamics, and various survey-based assessments (demographics, System Usability Scale, Simulator Sickness Questionnaire, and …

Process-Structure-Property Relationships Of Nanofibers For Biomedical Applications, Dipasree Bhowmick

Theses and Dissertations

This thesis explores the fabrication, characterization, and applications of nanofiber membranes utilizing Forcespinning® technology. Two distinct nanofiber systems were developed and evaluated, one focusing on pH-responsive Eudragit-baicalin loaded nanofibrous membranes for wound healing and the second one on thermo-responsive nanofiber-hydrogel (PNIPAAm) systems for biosensing applications, specifically dopamine detection. In the first project, nanofiber membranes loaded with Eudragit and baicalin demonstrated superior antibacterial properties against both gram-negative (E. coli) and gram-positive (B. meg) bacteria. Additionally, these membranes exhibited antioxidant properties and enhanced cell proliferation of 3TT cells, making them promising candidates for wound healing dressings. In the second project, a …

Using Noninvasive Calibrated Cuff Plethysmography To Observe The Effects Of Cold-Water Immersion On Arterial Compliance, Rita M. Grigorian

Biomedical Engineering: Graduate Reports and Projects

As the prevalence of cardiovascular diseases continues to exponentially grow in populations across the globe, the necessity of determining underlying factors, effective methods of diagnoses, and universally available preventive measures also grows. Early detection of endothelial dysfunction, a proven precursor of cardiovascular diseases, can be extremely impactful in encouraging preventative measures and early intervention before medical conditions become chronic. In recent years, ice plunging, a form of cryotherapy involving full body immersion in cold water, has gained popularity within circles of fitness and health practitioners, gaining the interest of people of all backgrounds. Certain parallels observed between the human physiological …

Post-Wildfire Export Regimes Of Solutes Along The Gallinas-Pecos River-Santa Rosa Fluvial Network, Asmita Kaphle

Civil Engineering ETDs

Wildfires significantly contribute to water pollution when ash and sediments, including nutrients and metals, are released into fluvial systems, impacting aquatic ecosystems and surrounding communities. This study focuses on the impacts of the Hermits Peak-Calf Canyon Fire on the Gallinas and Pecos watersheds. It employs five monitoring stations along a 190km fluvial network to assess solute export regimes post-fire, where grab samples were collected for nutrient and metal analyses for a period of more than a year. The metrics used for characterizing mobilization, chemostatic, or dilution patterns indicated mobilization for most solutes at all sites upstream of Santa Rosa Lake. …

Enhancing Urban Water Quality Through Biological-Chemical Treatment: Aquatic Macroinvertebrate Community And Temporal Chlorophyll-A Response, Matthew Chaffee

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

With a growing human population, urbanization is impeding a plethora of natural waterways. Of these, urban ponds play a vital role in nutrient sequestration, flood prevention, and habitat sanctuaries. However, nutrient loading can reduce habitat effectiveness and promote harmful algae blooms. To reduce internal nutrient loads, a biological-chemical treatment strategy consisting of floating treatment wetlands (FTWs) and lanthanum were applied to two urban retention ponds, Densmore and Wilderness Ridge Ponds. To measure effectiveness, chlorophyll-a samples were collected and correlated with Sentinel-2. A novel band algorithm termed 3BR1 produced a strong correlation (R² = 0.72) to physical chlorophyll-a …

Responses Of Maize To Different Irrigation Regimes In Semi-Arid Western Nebraska, Swathi Palle

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

As the “Cornhusker State”, Maize (Zea mays) is an important crop in Nebraska. However, farmers in the state are challenged by unstable supply of surface water and limited groundwater resources. A better understanding of maize's response to water stress that occurs at different growth stages can help implement the best irrigation practices that conserve water while maintaining yields. In this study, we conducted field experiments at the Panhandle Research, Extension, and Education Center to compare the responses of maize to eight irrigation treatments which included both limited and deficit irrigation regimes during 2022 and 2023 growing seasons. Specifically, …

Renovating The Ipmu Via Internet Of Things For Pollutant Emission Estimations In Poultry Facilities, Joshua Dotto

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The emissions of ammonia (NH₃), particulate matter (PM_2.5), and carbon dioxide (CO₂) are major concerns in poultry facilities. They can pose environmental concerns and nuances. Robust and affordable measurement systems are needed to accurately measure in-barn concentrations and quantify the emissions.

The Intelligent Portable Monitoring Unit (iPMU or PMU3) developed in 2016 was reconstructed into PMU4 to include upgraded NH₃ and PM_2.5 sensors and wireless connectivity for a low-cost, robust, and accurate air quality monitoring device with contactless data transfer using the concept of Internet of Things (IoT). In addition, a user-friendly …

Molecular Control Of Triplet-Pair Spin Polarization And Its Optoelectronic Magnetic Resonance Probes, Obadiah G. Reid, Justin C. Johnson, Joel D. Eaves, Niels H. Damrauer, John E. Anthony

Chemical and Materials Engineering Faculty Publications

Preparing and manipulating pure magnetic states in molecular systems are the key initial requirements for harnessing the power of synthetic chemistry to drive practical quantum sensing and computing technologies. One route for achieving the requisite higher spin states in organic systems exploits the phenomenon of singlet fission, which produces pairs of triplet excited states from initially photoexcited singlets in molecular assemblies with multiple chromophores. The resulting spin states are characterized by total spin (quintet, triplet, or singlet) and its projection onto a specified molecular or magnetic field axis. These excited states are typically highly polarized but exhibit an impure spin …

Quantifying Impacts Of Climate Change On Headwater Streamflow Regime In Robinson Forest: Insights From 35-Years Of Data Collection., Lauren Brown

Electronic Theses and Dissertations

Climate change may shift patterns of streamflow permanence in headwater systems by altering the frequency, magnitude, duration, timing, and rate of change of surface streamflow, impacting both local ecosystems as well as regional water budgets and availability. While much uncertainty surrounds modeling-based methods to quantify the impacts of climate change on water budgets, long-term hydrologic data collected from headwaters in experimental research forests serve as critical evidence to reduce such uncertainty. The objective of this study is to quantify shifts in frequency, magnitude, duration, timing, and rate of change of streamflow in two headwater catchments with relatively little recent disturbance …

Robust And Uncertainty-Aware Image Classification Using Bayesian Vision Transformer Model, Fazlur Rahman Bin Karim

Theses and Dissertations

Transformer Neural Networks have emerged as the predominant architecture for addressing a wide range of Natural Language Processing (NLP) applications such as machine translation, speech recognition, sentiment analysis, text anomaly detection, etc. This noteworthy achievement of Transformer Neural Networks in the NLP field has sparked a growing interest in integrating and utilizing Transformer models in computer vision tasks. The Vision Transformer (ViT) model efficiently captures long-range dependencies by employing a self-attention mechanism to transform different image data into meaningful, significant representations. Recently, the Vision Transformer (ViT) has exhibited incredible performance in solving image classification problems by utilizing ViT models, thereby …

Using Statistical Clustering Of Trajectory Data To Support Analysis Of Subject Movement In A Virtual Environment, Martín Alejandro Galicia Avila

Theses and Dissertations

The rising use of virtual reality software requires new methods of spatiotemporal analysis to enhance the user experience. This investigation is focused on forming clusters from spatiotemporal trajectories within a virtual environment and analyzing them in search of human immersion and behavior insights. The cluster centroids are plotted and analyzed to identify associations with the trajectories and user demographics.

Gaze Tracking Embedded Collaborative Robots For Automated Metrology And Reverse Engineering, Sachithra H. Karunathilake

Theses and Dissertations

Conventional geometric metrology, or three-dimensional (3D) scanning, and reverse engineering heavily rely on the experience of the operators. With an increasing need for automation, robot arms have been adopted for this task. However, due to the large variety of parts and designs, automated path planning could provide a scanning solution that may overlook the critical area, which could potentially deteriorate the scan results. This study explores the integration of collaborative robotics (cobots) with eye tracking technology to improve the autonomous 3D scanning process. The primary objective of this study is to enhance the accuracy and efficiency of cobots in …

Design And Characterization Of A Novel Eef2k Degrader With Potent Therapeutic Efficacy Against Triple-Negative Breast Cancer, Changxin Zhong, Rongfeng Zhu, Ting Jiang, Sheng Tian, Xiaobao Zhao, Xiaoya Wan, Shilong Jiang, Zonglin Chen, Rong Gong, Linhao He, Jin-Ming Yang, Na Ye, Yan Cheng

Markey Cancer Center Faculty Publications

Dysregulated eEF2K expression is implicated in the pathogenesis of many human cancers, including triple-negative breast cancer (TNBC), making it a plausible therapeutic target. However, specific eEF2K inhibitors with potent anti-cancer activity have not been available so far. Targeted protein degradation has emerged as a new strategy for drug discovery. In this study, a novel small molecule chemical is designed and synthesized, named as compound C1, which shows potent activity in degrading eEF2K. C1 selectively binds to F8, L10, R144, C146, E229, and Y236 of the eEF2K protein and promotes its proteasomal degradation by increasing the interaction between eEF2K and the …

1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (Mptp)-Treated Adult Zebrafish As A Model For Parkinson’S Disease, Emmeline Bagwell

All Theses

The use of adult zebrafish, Danio rerio, as a model for human neurodegenerative diseases has proven valuable in pharmaceutical development and genetic disease research due to their high genetic homology to humans, cost-effective husbandry, and quick life cycle breeding times. Despite their promise, most zebrafish research has focused on embryonic stage organisms, primarily due to the ease of direct handling protocols and rapid progression through developmental stages. Recognizing the limitations of early-stage organisms in modeling adult diseases, recent efforts have shifted the applicability of zebrafish models, particularly for Parkinson's Disease, due to the nature of the disease impacting patients 55 …