Digital Commons Network^™

Drop Retention And Departure In Shear Flow On Structured Superhydrophobic Surfaces, Blake Morgan Lyons

Theses and Dissertations

Water is often found adhering to surfaces in droplet form. Droplets can develop as a result of precipitation, condensation, or chemical reactions. Drops are retained or held on a surface due to a retention force exerted on the drop by the surface. This retention force is a function of the surface tension of the liquid, drop geometry, and the contact angle between the drop and the surface. When external or body forces exceed this retention force the drop begins to move. This thesis seeks to understand and quantify the forces present at the point of droplet departure once it begins …

Advancements In Levitated Optomechanics: Exploring Plasmon-Enhanced Light-Matter Interactions In Optical Trapping, Siavash Mirzaei Ghormish

Theses and Dissertations

Optical trapping of metallic nanoparticles investigates phenomena at the interface of plasmonics and optical micromanipulation. This thesis combines ideas from the optical properties of metals, originating in solid-state physics, with the force mechanisms resulting from optical trapping. I explore the influence of enhanced light-matter interaction due to the plasmon resonances of gold particles on their trapping properties. I present theories for trapping mechanisms of gold metallic particles and then verify these theories through experiments. Optical trapping of metallic nanoparticles is considered challenging, especially in air, as they are highly absorbing and reflecting at optical wavelengths. Yet the optical levitation of …

Systematic Comparison Of Nonviral Gene Delivery Strategies For Efficient Co-Expression Of Two Transgenes In Human Mesenchymal Stem Cells, Tyler Kozisek, Luke Samuelson, Andrew Hamann, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background Human mesenchymal stem cells (hMSCs) are being researched for cell-based therapies due to a host of unique properties, however, genetic modification of hMSCs, accomplished through nonviral gene delivery, could greatly advance their therapeutic potential. Furthermore, expression of multiple transgenes in hMSCs could greatly advance their clinical significance for treatment of multifaceted diseases, as individual transgenes could be expressed that target separate pathogenic drivers of complex diseases. Expressing multiple transgenes can be accomplished by delivering multiple DNA vectors encoding for each transgene, or by delivering a single poly-cistronic vector that encodes for each transgene and accomplishes expression through either use …

Exploring Regular And Turbulent Flow States In Active Nematic Channel Flow Via Exact Coherent Structures And Their Invariant Manifolds, Caleb G. Wagner, Rumayel H. Pallock, Jae Sung Park, Michael M. Norton, Piyush Grover

Department of Mechanical and Materials Engineering: Faculty Publications

This work is a unified study of stable and unstable steady states of 2D active nematic channel flow using the framework of Exact Coherent Structures (ECSs). ECSs are stationary, periodic, quasiperiodic, or traveling wave solutions of the governing equations that, together with their invariant manifolds, organize the dynamics of nonlinear continuum systems. We extend our earlier work on ECSs in the preturbulent regime by performing a comprehensive study of stable and unstable ECSs for a wide range of activity values spanning the preturbulent and turbulent regimes. In the weakly turbulent regime, we compute more than 200 unstable ECSs that coexist …

Coarsening Dynamics Of Ternary Polymer Solutions With Mobility And Viscosity Contrasts, Jan Ulric Garcia, Douglas R. Tree, Alyssa Bagoyo, Tatsuhiro Iwama, Kris T. Delaney, Glenn H. Fredrickson

Faculty Publications

Using phase-field simulations, we investigate the bulk coarsening dynamics of ternary polymer solutions undergoing a glass transition for two models of phase separation: diffusion only and with hydrodynamics. The glass transition is incorporated in both models by imposing mobility and viscosity contrasts between the polymer-rich and polymer-poor phases of the evolving microstructure. For microstructures composed of polymer-poor clusters in a polymer-rich matrix, the mobility and viscosity contrasts significantly hinder coarsening, effectively leading to structural arrest. For microstructures composed of polymer-rich clusters in a polymer-poor matrix, the mobility and viscosity contrasts do not impede domain growth; rather, they change the transient …

Biological Nitrogen And Phosphorus Removal From Wastewater Under Low Dissolved Oxygen (Ldo) Conditions, Bashar Al-Daomi

Dissertations and Theses

This study examines the complexities of the integration of different technologies for nutrient removal, investigating parameters like sludge residence time (SRT), carbon-to-nitrogen (C/N) ratio, Anaerobic/Oxic (A/O), and Anaerobic/Oxic/Anoxic (A/O/A) systems, continuous and intermittent aeration regimes, and air OFF/air ON periods ratios for efficient nitrogen and phosphorus removal.

Two continuous aeration systems were implemented: The first aimed to carry out a simultaneous nitrification and denitrification (SNdN) process for nitrogen and phosphorus removal under low dissolved oxygen (DO) and C/N ratio conditions. This system operated with 4.5-5-day total sludge age in a single-stage anaerobic/oxic (A/O)-SBR, performing stepwise DO reduction at varying DO …

An Inexpensive, 3d Printable, Arduino And Bluray-Based, Confocal Laser And Fluorescent Scanning Thermal Microscope, Justin Loose

Theses and Dissertations

The Fluorescence Scanning Thermal Microscope (FSTM v3.0), was designed to create an inexpensive, and easily manufactured, device for measuring the diffusivity of samples with microscopic locational precision. This was accomplished by using a Blu-ray device known as a PHR-803T, referred to in this work as a PHR. The optics in the PHR are nearly identical in function to conventional devices used in thermoreflectance microscopy, making the PHR extremely useful to integrate into the FSTM design. The focus of this thesis is the application of the FSTM as a confocal microscope using 3D printed components and various low-cost devices to operate …

Predictive Modeling Of Asphalt Pavement Performance Using Artificial Neural Networks, Rami Khalifah

Civil Engineering Theses

This thesis highlights the significance of using the Resilient Modulus (Mr) and Soil-Water Characteristic Curve (SWCC) coefficients within the scope of pavement design. It aims to demonstrate the importance of pavement quality and safety by developing a prediction model for bleeding and rutting depth in asphalt pavements using data from the Long-Term Pavement Performance (LTPP) database and the National Catalog of Natural Subgrade Properties Database. Additionally, the thesis builds predictive models using Artificial Neural Networks (ANNs), which include a single hidden layer and multiple neurons. The developed Mr and SWCC models in this thesis utilized the basic soil index properties …

Quaternary Ammonium Compounds (Qacs) In Wastewater Influent And Effluent Throughout The Covid-19 Pandemic, Anna K. Mahony, Patrick J. Mcnamara, William A. Arnold

Civil and Environmental Engineering Faculty Research and Publications

Quaternary ammonium compounds (QACs) are used in consumer and industrial products, including disinfectants. Due to the COVID-19 pandemic, disinfectant use has increased, purportedly increasing loads to wastewater treatment plants and the environment. To understand how the increased usage has affected QAC loadings to treatment plants and to determine how effectively plants remove QACs from liquid effluent that is discharged to surface and groundwaters, influent and effluent wastewater samples were collected from four treatment plants (treatment capacities < 5 MGD to > 100 MGD) for 21 months beginning in May 2020. Influent QAC concentrations were hundreds of μg/L and effluent QAC concentrations were < 1 μg/L, corresponding to an average removal of 98% from all four plants. The most prevalent QACs in influent were those used most commonly in disinfectants, specifically benzylalkyldimethylammonium compounds (BACs) and short-chain dialkyldimethylammonium compounds (DADMACs), and influent levels of these compounds were correlated with QAC sales. Prior to this study, ethylbenzylalkyldimethylammonium compounds (EtBACs) had not been studied, and they comprised 13 ± 6% of QACs in influent. While removal was high at all plants, low μg/L concentrations were still continuously discharged into the environment. For QACs with equivalent alkyl chain lengths, those with aromatic substituents (BACs and EtBACs) appear to be removed more effectively than those with only alkyl chains (DADMACs).

Microwave-Photonic Vernier Effect Enabled High-Sensitivity Fiber Bragg Grating Sensors For Point-Wise And Quasi-Distributed Sensing, Chen Zhu, Osamah Alsalman, Jie Huang

Electrical and Computer Engineering Faculty Research & Creative Works

This paper reports a sensitivity-improved fiber Bragg grating (FBG) sensor system based on microwave-photonic interferometry and the Vernier effect. an incoherent microwave photonics system based on a broadband light source is employed to interrogate the FBG sensor using the wavelength-to-delay mapping technique combined with interferometry. Specifically, the sensing FBG together with a reference FBG is used to construct a microwave photonics Michelson interferometer (MI). Changes in the Bragg wavelength of the sensing FBG subject to external perturbations are encoded into the spectral shifts of the microwave interferogram of the MI. a virtual interferometer is then generated from the sensing MI …

Effects Of Beet Supplements On Cardiovascular Response Using A Noninvasive Blood Pressure Cuff, Nicholas M. Hughes

Master's Theses

A Calibrated Cuff Plethysmography device was built, tested for verification, and used to experiment on human subjects to measure the cardiovascular response of consuming a beet supplement, specifically looking at arterial compliance and pressure-area curves. Each subject was tested four times. A baseline was measured under normal conditions and after five-minute hyperemia conditions. 10 subjects were given 6 ounces of water mixed with either purple Kool-Aid (control), a SuperBeets supplement, or a SuperBeets Sport supplement and after 45 minutes, measurements were taken undergoing normal and hyperemia conditions once more. The verification testing demonstrated the calibration of the device was effectively …

A Low-Power Analog Cell For Implementing Spiking Neural Networks In 65 Nm Cmos, John S. Venker, Luke Vincent, Jeff Dix

Computer Science and Computer Engineering Faculty Publications and Presentations

A Spiking Neural Network (SNN) is realized within a 65 nm CMOS process to demonstrate the feasibility of its constituent cells. Analog hardware neural networks have shown improved energy efficiency in edge computing for real-time-inference applications, such as speech recognition. The proposed network uses a leaky integrate and fire neuron scheme for computation, interleaved with a Spike Timing Dependent Plasticity (STDP) circuit for implementing synaptic-like weights. The low-power, asynchronous analog neurons and synapses are tailored for the VLSI environment needed to effectively make use of hardware SSN systems. To demonstrate functionality, a feedforward Spiking Neural Network composed of two layers, …

Bioactivity And Structural Properties Of Hydroxyapatite On Ti6a|4v And Si(100) Surfaces By Pulsed Laser Deposition, Salizhan Kylchbekov

Masters Theses & Specialist Projects

Although biomedical implant technology is very advanced, there are still caveats in terms of biocompatibility properties because metals are inert to biological processes such as osseointegration, cell growth, and cell adhesion. This results in statistically significant complications and opens rooms for improvements. Among many techniques to improve this problem, coating the metal surface with biologically functional materials has resulted in the best performances. Hydroxyapatite (HAP), Ca_₁₀(PO_₄)_₆(OH)_₂, is the most stable form of calcium phosphates in human body environment and is functional in key processes such as ion exchange, osteoblast (bone cells) formation, …

Perfusability And Immunogenicity Of Implantable Pre-Vascularized Tissues Recapitulating Features Of Native Capillary Network, Dhavan Sharma, Archita Sharma, Linghao Hu, Te An Chen, Sarah Voon, Kayla J. Bayless, Jeremy Goldman, Alex J. Walsh, Feng Zhao

Michigan Tech Publications

Vascularization is a key pre-requisite to engineered anatomical scale three dimensional (3-D) constructs to ensure their nutrient and oxygen supply upon implantation. Presently, engineered pre-vascularized 3-D tissues are limited to only micro-scale hydrogels, which meet neither the anatomical scale needs nor the complexity of natural extracellular matrix (ECM) environments. Anatomical scale perfusable constructs are critically needed for translational applications. To overcome this challenge, we previously developed pre-vascularized ECM sheets with long and oriented dense microvascular networks. The present study further evaluated the patency, perfusability and innate immune response toward these pre-vascularized constructs. Macrophage-co-cultured pre-vascularized constructs were evaluated in vitro to …

Exploration Of Secondary Structure Directing Agents On The Formation Of Hierarchical And 2d Zeolites, Suleiman Yahaya

ETD Archive

Hierarchical and 2D zeolites address the diffusion limitation of conventional zeolites by enhancing diffusion in the mesopores. So far, the surfactant compounds, which successfully produce hierarchical zeolites, have complex molecular structures making the zeolite synthesis expensive and uncompetitive for commercialization, making cost-effective synthesis of hierarchical and 2D zeolite highly desirable. However, the development of effective synthesis protocols relies on a good understanding of the SDA's influence on crystal morphology development, which is largely unknown. Here, we explore inexpensive and readily available compounds via a dual-SDA strategy and use MFI zeolite as the model system for the research. TPAOH served as …

4d Printing Of Pla And Pla-Based Composites For Load-Bearing Applications, Phaniteja Nagaraju

ETD Archive

This research investigates the deformation recovery of 3D-printed Polylactic Acid (PLA) shape memory polymers (SMPs). The study delves into the influence of Fused Deposition Modelling (FDM) parameters, such as nozzle temperature, layer thickness, and printing speed, on the thermomechanical behavior of PLA SMP honeycomb structures. The research identifies optimal printing conditions using the Taguchi approach and explores the cyclic shape memory recovery behavior of the optimized PLA SMP under compression loads.

Additionally, the research includes a chapter on the extrusion and printing of uniform-diameter polylactic acid carbon fiber (CFR-PLA) composite filaments. The synthesis of CFR-PLA involves planetary ball milling for …

Treatment Of Combined Dye And Protein Wastewater By Coagulation Process, Siriwat Tanongsak

ETD Archive

The treatment of combined dye and protein wastewater is a major environmental concern, due to its complex composition and high organic content. In this study, the effectiveness of the coagulation process for the treatment of combined dye and protein wastewater was investigated. Results of the study showed that the coagulation process was effective in removing suspended solids, color, and organic matter from the combined dye and protein wastewater. The removal efficiency varied depending on the type and dosage of the coagulant used. Among the coagulants tested, alum and ferric chloride were found to be the most effective in removing suspended …

Methods For Objective Determination Of Musculoskeletal Coordinate Systems, Tara Francesca Nagle

ETD Archive

Joint mechanics research relies on joint kinematics and kinetics measurements, represented from relative relationships of local coordinate systems (CS) belonging to bones of the joint. It’s common to define these CSs from anatomical landmarks, which are sensitive to observer variability and often don’t result in CS that best represent the functional motion of the joint. This work is presented in three articles addressing the following aims: 1) to develop a method to objectively define coordinate systems through optimization of unique passive movement paths, 2) to develop an alternative method to objectively define coordinates systems for joints with non-unique passive movement …

Spatially Resolved Elastic Modulus Of Magnesium Silicate Hydrate: A Cementitious Material, Arif Syed

Material Science and Engineering Theses - Archive

Magnesium-based cements such as magnesium silicate hydrate (MSH) have drawn interest as an environmentally friendly substitute for ordinary Portland cement because of their potential for reduced carbon footprint. The precise determination of these materials' elastic moduli is important to better assess their mechanical performance. Atomic force microscopy (AFM) is an effective tool for precise and spatially resolved quantification of nanomechanical characteristics of materials, including thin films. In this study, elastic modulus maps of MSH grown on single crystal mica surfaces were obtained using amplitude modulation-frequency modulation AFM. The effects of the Mg:Si ratio and morphology on the elastic modulus of …

On The Development Of A Sensing System Methodology To Evaluate The Viscoelastic Properties Of Soft Tissues As A Means Of Disease Prognosis, Shashank S. Kumat

Mechanical and Aerospace Engineering Dissertations - Archive

Identification of tissue viscoelastic properties could provide valuable information for assessing its healthiness or disease state. Current technologies present challenges to access and perform localized tissue assessment in confined spaces in the human body through contact indentation/palpation. As such, there is a need for a diagnostic system capable of measuring tissue relaxation response at the local site by accessing the tissue through a natural orifice. This dissertation presents a strain gauge-based uniaxial micro-force sensor, part of the aforementioned system, capable of measuring tissue response data in confined human space environments. A sensing system design methodology is developed and presented. The …

Optimizing Energy Efficiency And Performance In High-Performance Computing Through Advanced Direct-To-Chip Liquid Cooling And Labview-Based Monitoring And Analysis, Chandraprakash Ashok Hinge

Mechanical and Aerospace Engineering Dissertations - Archive

This research endeavors to significantly enhance energy efficiency and performance in the realm of high-performance computing (HPC). This goal is pursued through a synergistic approach that leverages advanced direct-to-chip liquid cooling systems and integrates LabVIEW for real-time monitoring and analysis within data centers. The primary aims of this study are manifold. First, it seeks to evaluate the effectiveness of different single-phase liquid coolants when employed in direct-to-chip cold plate cooling for HPC systems. Additionally, the research focuses on characterizing row manifolds, crucial elements in the design of efficient data centers. The study also endeavors to devise a strategic control mechanism …

Lung Cancer Growth Forecasting And Classification Models By Machine Learning Using A Novel 2d Co-Culture High Throughput Device, Adam Adrien Germain

Bioengineering Dissertations - Archive

Co-culture devices have commonly been used during in vitro experimentation to create therapeutic testing devices for lung cancer that more closely mimic the in vivo environment to gain better insights into new therapies and the reasons for inconsistency for current therapies. Moreover, AI (artificial intelligence) in the form of convolutional neural networks (CNN) and convolutional long-short term memory neural networks (convLSTM) are being used to classify, diagnose, and predict drug outcomes from the visual data provided by clinical imaging modalities, such as CT-scans, MRIs, or histopathological slides among others, or from numerical and textual data gathered from the genetic testing …

Advances And Applications In Ultrasound-Switchable Fluorescence Imaging, Liqin Ren

Bioengineering Dissertations - Archive

Cancer diagnosis is a prerequisite for further medical therapy and treatment. Near-infrared (NIR) fluorescence imaging is a desirable modality without invasiveness and nonionizing radiation. However, fluorescence suffers from a strong light scattering in tissues, leading to a low spatial resolution and low sensitivity in centimeter-deep tissue (limited to several millimeters). To enhance spatial resolution and avoid the high scattering of conventional fluorescence imaging in deep tissue, ultrasound-switchable fluorescence (USF) imaging has emerged as a highly promising technique for biomedical imaging and research. The principle of improving spatial resolution in USF imaging is to confine the fluorescence emission from thermosensitive fluorophores …

Development Of Multiscale And Multidimensional Light Sheet Microscopy For Image-Based Modelling Of Organogenesis, Tanveer Ashwini Ashwini Teranikar

Bioengineering Dissertations - Archive

Behavioral analysis of individual cells, in the form of structural phenotypes arising in response to mechanical stimuli or genomic alterations as small molecule intermediates of disease, are instrumental for understanding organogenesis. In this regard, dynamic cell tracking or temporal changes in cell shape, area and size are vital for modelling tissue interactions. However, optical diffraction limits imposed by lenses, hinders high resolution volume reconstruction of microscale biological processes and subcellular organelles. Thereby, isotropic intensity is restricted to limited focal volumes, reducing imaging distance to less than 1 mm for high magnification. To combat this, Light Sheet Microscopes (LSM) have emerged …

Quantification Of Dynamic Changes Of Hemodynamic And Metabolic Responses To Brachial Artery Occlusion: A Broadband Near-Infrared Spectroscopy Study, Caroline Elizabeth Carter

Bioengineering Theses - Archive

Cardiovascular disease (CVD) is a leading cause of death in the United States, and non-Hispanic African Americans are at higher risk than other racial and ethnic groups. Endothelial dysfunction is a major contributor to CVD development and progression. Remote ischemic pre/conditioning (RIC), which involves exposing cells or tissues to brief sublethal stress, has been shown to protect cardiovascular cells from ischemia and reperfusion injury, including CVD. This study aimed to investigate hemodynamic and metabolic dynamics in response to 5-min forearm arterial occlusion using broadband near-infrared spectroscopy. My thesis is a tripartite investigation that weaves RIC, mitochondrial dynamics, and health disparities …

Formation And Morphological Changes Of Stress Actin Filaments In Adipose-Derived Stem Cells, Adam Priester

Bioengineering Theses - Archive

Adipose-Derived Stem Cells (ADSCs) are a sub-type of stem cell that can be collected and isolated from the adipose tissue. Adipogenesis describes the commitment and differentiation of stem cells, including ADSCs, toward the adipocyte lineage. While much research has been completed on this process in the long term (>2 weeks), morphological changes of adipogenesis during the early phase (<24 hours) are lacking in the literature and remain to be elucidated. The current study focuses on these changes in the early and later stages of adipogenesis using adipogenic differentiation factors and fluorescent imaging analyses. Quantifiable data on morphological changes during both phases can provide insight into how these changes affect later differentiation and lay the groundwork for additional research in this area. Using fluorescent image analysis, it was shown that the eccentricity of ADSCs, a measure of cellular circularity, decreases exponentially in the early phase of adipogenesis. In contrast, the cellular area appears to increase exponentially during the same early period. During the later stages of differentiation, it was shown that actin filament length decreases exponentially and the ratio of lipid to actin length increases exponentially, demonstrating that lipid accumulation outpaces actin filament shortening and increasing circularity during the later stages. These findings may be used in future studies to further define the role of actin filament-mediated morphological changes and mechanobiology during adipogenesis.

Evaluation Of Vertical And Lateral Load Capacity Of Recycled Plastic Pin, Sehneela Sara Aurpa

Civil Engineering Dissertations - Archive

RPPs have been in use as a sustainable and effective technique to stabilize and reinforce weak ground for geotechnical purposes. Application of RPP in stabilization of slopes and improvement of load carrying capacity of foundation soil has yielded tremendous results. However, to facilitate the seamless integration of RPPs in design of foundation requires the load carrying capacity of RPP to be known. As RPP has been in use for both its vertical and lateral load carrying capacity, exploration of the load capacity of this material is essential. The objective of the study is to determine the load carrying capacity of …

Utilization Of Biomimetic Molecules To Control The Carbonation Of Cementitious Phases, Muhammad Intesarul Haque

Civil Engineering Dissertations - Archive

This study introduces an innovative approach to mitigate carbonation-induced degradation of C-S-H and to enhance the carbonation properties of low calcium based cementitious materials by utilizing specific biomimetic molecules. In this study, four different biomimetic molecules were utilized. Details about their chemical formula and chemical structure can be found in Chapter 1. The dissertation is broadly focused on two aspects, Part 1 (Chapter 2 and 3) evaluated the role of biomimetic molecules in reducing the carbonation degradation of C-S-H. Part 2 (Chapter 4 and 5) assessed the effectiveness of the biomimetic molecules as performance enhancing admixture of carbonation cured dicalcium …

“Increasing Renewable Energy Potential Of Landfill Gas By Electrocatalytic Conversion Of Carbon Dioxide To Methane At Low Temperature.”, Asma Akter Rony

Civil Engineering Dissertations - Archive

The conversion of carbon dioxide into methane at a low temperature has great potential to reduce current international environmental issues like global warming and will create new sources of renewable energy. The overall goal of this research is to increase renewable energy production from landfill gas using an unconventional process – electrocatalytic methanation of carbon dioxide – which has not been implemented for landfill gas yet. The electrocatalytic methanation follows the Waseda process, which applies an electric field in the presence of a Ruthenium-supported cerium oxide (CeO2) catalyst. The specific objectives of this research were: 1. To explore the impact …

The Effects Of Poloxamer 188 On Wound Closure In A Mouse Brain Endothelium Model, Mia Claire Grubbs

Bioengineering Theses - Archive

The blood-brain barrier (BBB) plays a crucial role in maintaining central nervous system homeostasis and, if structurally and functionally compromised, can lead to severe neurological consequences. This thesis explores the therapeutic potential of Poloxamer 188 (P188) in the context of BBB wound healing. P188, FDA-approved copolymer that is known for its cytoprotective properties, is examined for its impact on endothelial monolayers through in vitro scratch wound assays. The study aims to establish a consistent endothelial monolayer model, quantify wound healing dynamics, and assess the efficacy of P188 at varying concentrations. The thesis goals include detailed methodologies such as cell culture …