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A Novel In-Vitro Approach To Investigate The Molecular Interaction Between Inhaled Particles And The Human Pulmonary Surfactant System, Bryan Mayville

Browse all Theses and Dissertations

The deployment of military personnel to austere environments poses significant pulmonary health risks from inhalation of particulates including sand, dust, pollution, and heavy machinery exhaust. When particles are less than 10 µm in diameter, they can penetrate deep within the alveoli and embed within the protective lung surfactant monolayer. Here, absorption of surfactant lipids to the particle surface disrupts the essential monolayer configuration needed to reduce surface tension and prevent atelectasis, thus leading to disease. Currently, there are no in vitro surfactant-producing lung cell models capable of studying the effects of inhaled particles on the human pulmonary surfactant system. A549 …

Sex-Driven Alterations In Aging Alpha Motoneurons: Exploring Size, Type, Density, And Kv2.1 Channel Expression, Kalin R. Gerber

Browse all Theses and Dissertations

This research explores how motor neurons (MNs) and Kv2.1 clustering change with age, emphasizing sex differences and MN subtypes. We found that MN density decreases with age in both sexes, while soma size increases in male mice. FF MNs were the most affected, and old weak mice had smaller MNs than their stronger counterparts, underscoring FF MN vulnerability. Baseline studies revealed that FF and FI MNs have larger Kv2.1 clusters compared to FR and S MNs. Female mice had smaller, denser Kv2.1 clusters than male mice, suggesting less clustering in females. With age, Kv2.1 clusters grew larger but became less …

Development Of Novel Hydrogel Materials For Viscoelastically Accurate Brain Phantoms, Lila G. Schandler

Theses and Dissertations

Current methods such as concussion tests and Magnetic Resonance Imaging (MRI) scans only reveal structural damage post-injury. Creating an anatomically accurate model of the brain that mimics brain structure and viscoelastic properties will further allow medical professionals to analyze concussion events and create curated and personalized plans of recovery for a patient’s unique brain injury.

Values have been established for the necessary viscoelastic properties (time constants and elasticity) to mimic the native human brain tissue such as grey matter and white matter. The mechanical properties of the hydrogel are tunable by varying the concentration of Phytagel (PHY), a protein binding …

Anaerobic Reductive Bioleaching Of Manganese Ores, Neha Sharma

Dissertations, Master's Theses and Master's Reports

Manganese extraction by biological methods is a green and economical way to produce manganese from low as well as high grade manganese ores. This is especially important for US ores, which are primarily either small in extent, low-grade with a high iron content, or both. They are therefore not suitable for conventional mining, which is very capital-intensive and requires a large up-front investment and are therefore imported at high costs. The goal is therefore to develop a process that can be applied at small scales, with minimal startup costs, and low equipment and labor requirements. It is also critical to …

Initial Solution Improvements To A Tethered Robotic Path Planner Using Random Geometric Graph Configurations And Biased Sampling, Austen J. Goddu

Dissertations, Master's Theses and Master's Reports

With NASA's ongoing efforts to establish a presence on the lunar surface to eventually move on to exploring mars, the development of intelligent robotic systems is more important than ever. The ability of robotics to explore hazardous and extreme terrain, coupled with long communication times from earth places an ever increasing need on more robust and efficient autonomy. Tethered robotics offer unique advantages to explore scientific targets both on the lunar and martian surfaces, capable of using their tether as a physical or metaphorical lifeline to allow the exploration of slopes, extreme dark regions, or areas in which wireless communication …

Adaptation Of A Commercially Available Galvanic Skin Response Sensor To Measure Respiration Across The Chest For Heart Rate Variability Monitoring, Breno C. Dobal

Honors Undergraduate Theses

Heart rate variability (HRV) is a naturally occurring cardiovascular phenomenon referring to the changing timing between consecutive heartbeats. The connection between HRV and overall cardiovascular health and autonomic nervous system function has been well established through prior research and well documented in existing literature. The existing studies, however, included shorter HRV subject recording session, using traditional HRV monitoring methods that do not typically combine electrocardiogram (ECG), seismocardiogram (SCG) and galvanic skin response (GSR) respiration monitoring. The inclusion of longer HRV subject recording may allow for further insight on the possible effects of given observable biological phenomenon on HRV.

The current …

Designing A Visual Control Framework Based On Feature Point Analysis To Guide Robots In Uncharted Areas, Isam Sadiq Rasham Al-Ghadhbawi

Al-Esraa University College Journal for Engineering Sciences

Image processing may be made more accurate and efficient with the use of optical processing technologies. In order to make better design judgments early in the package design process, visual packaging art simulation aims to swiftly develop realistic packaging effects. Conventional simulation techniques frequently yield findings that are neither realistic or detailed, and they also take a lot of time and human resources. Optical processing-based machine vision technology can fully utilize computer vision and image processing techniques to simulate package designs accurately and efficiently. This research proposes a foundation for machine vision technologies based on image optical processing. Real package …

Perfluorooctanesulfonic Acid Exposure Leads To Downregulation Of 3-Hydroxy-3-Methylglutaryl-Coa Synthase 2 Expression And Upregulation Of Markers Associated With Intestinal Carcinogenesis In Mouse Intestinal Tissues, Josiane Weber Tessmann, Pan Deng, Jerika Durham, Chang Li, Moumita Banerjee, Qingding Wang, Ryan A. Goettl, Daheng He, Chi Wang, Eun Y. Lee, B. Mark Evers, Bernhard Hennig, Yekaterina Y. Zaytseva

Markey Cancer Center Faculty Publications

Perfluorooctanesulfonic acid (PFOS) is a widely recognized environment pollutant known for its high bio- accumulation potential and a long elimination half-life. Several studies have shown that PFOS can alter multiple biological pathways and negatively affect human health. Considering the direct exposure to the gastrointestinal (GI) tract to environmental pollutants, PFOS can potentially disrupt intestinal homeostasis. However, there is limited knowledge about the effect of PFOS exposure on normal intestinal tissues, and its contribution to GI- associated diseases remains to be determined. In this study, we examined the effect of PFOS exposure on the gene expression profile of intestinal tissues of …

Cross-Layer Design Of Highly Scalable And Energy-Efficient Ai Accelerator Systems Using Photonic Integrated Circuits, Sairam Sri Vatsavai

Theses and Dissertations--Electrical and Computer Engineering

Artificial Intelligence (AI) has experienced remarkable success in recent years, solving complex computational problems across various domains, including computer vision, natural language processing, and pattern recognition. Much of this success can be attributed to the advancements in deep learning algorithms and models, particularly Artificial Neural Networks (ANNs). In recent times, deep ANNs have achieved unprecedented levels of accuracy, surpassing human capabilities in some cases. However, these deep ANN models come at a significant computational cost, with billions to trillions of parameters. Recent trends indicate that the number of parameters per ANN model will continue to grow exponentially in the foreseeable …

Nonuniform Sampling-Based Breast Cancer Classification, Santiago Posso

Theses and Dissertations--Electrical and Computer Engineering

The emergence of deep learning models and their success in visual object recognition have fueled the medical imaging community's interest in integrating these algorithms to improve medical diagnosis. However, natural images, which have been the main focus of deep learning models and mammograms, exhibit fundamental differences. First, breast tissue abnormalities are often smaller than salient objects in natural images. Second, breast images have significantly higher resolutions but are generally heavily downsampled to fit these images to deep learning models. Models that handle high-resolution mammograms require many exams and complex architectures. Additionally, spatially resizing mammograms leads to losing discriminative details essential …

Inaugural Future Of Bse Days: Workshop Report, Mark Stone

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

The inaugural Future of BSE Days (FBD) event, held on May 2-3, 2024, brought together the Biological Systems Engineering (BSE) community to discuss and shape the future of work and learning within the field. The event was designed to foster collaboration between faculty, students, industry leaders, and alumni, with the goal of reimagining the department’s educational approach, enhancing industry partnerships, and preparing students for the challenges of tomorrow. Over the course of two days, participants engaged in insightful discussions, workshops, and presentations that highlighted both the strengths of the department and opportunities for growth.

The workshop resulted in several key …

Stage And Discharge Prediction From Documentary Time-Lapse Imagery, Kenneth W. Chapman, Troy E. Gilmore, Mehrube Mehrubeoglu, Christian D. Chapman, Aaron R. Mittelstet, John E. Stranzl Jr.

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Imagery from fixed, ground-based cameras is rich in qualitative and quantitative information that can improve stream discharge monitoring. For instance, time-lapse imagery may be valuable for filling data gaps when sensors fail and/or during lapses in funding for monitoring programs. In this study, we used a large image archive (> 40,000 images from 2012 to 2019) from a fixed, ground-based camera that is part of a documentary watershed imaging project (https://plattebasintimelapse.com/). Scalar image features were extracted from daylight images taken at one-hour intervals. The image features were fused with United States Geological Survey stage and discharge data as …

Life Cycle Greenhouse Gas Emissions In Maize No-Till Agroecosystems In Southern Brazil Based On A Long-Term Experiment, Guilherme Rosa Da Silva, Adam J. Liska, Cimelio Bayer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Brazilian agriculture is constantly questioned concerning its environmental impacts, particularly greenhouse gas (GHG) emissions. This research study used data from a 34-year field experiment to estimate the life cycle GHG emissions intensity of maize production for grain in farming systems under no-tillage (NT) and conventional tillage (CT) combined with Gramineae (oat) and legume (vetch) cover crops in southern Brazil. We applied the Feedstock Carbon Intensity Calculator for modeling the “field-to-farm gate” emissions with measured annual soil N₂O and CH₄ emissions data. For net CO₂ emissions, increases in soil organic C (SOC) were applied as a proxy, …

Student Use Of Anchors And Metacognitive Strategies In Reflection, Anu Singh, Heidi A. Diefes-Dux

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Context: Self-regulation, a skillset involving taking charge of one’s own learning processes, is crucial for workplace success. Learners develop self-regulation skills through reflection where they recognize weaknesses and strengths by employing metacognitive strategies: planning, monitoring, and evaluating. Use of anchors assists learners’ engagement in reflection. Purpose or Goal: The purpose of this work was to gain insight into students’ use of anchors when reflecting on their learning. The two research questions: (1) To what extent do students link their self-evaluation and learning objective (LO) self-ratings to their reflections? and (2) What dimensions and level of metacognitive strategies do students use …

Classification Of Sow Postures Using Convolutional Neural Network And Depth Images, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Yeyin Shi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The United States swine industry reports an average preweaning mortality of approximately 16% where approximately 6% of them are attributed to piglets overlayed by sows. Detecting postural transitions and estimating sows’ time budgets for different postures are valuable information for breeders and engineering design of farrowing facilities to eventually reduce piglet death. Computer vision tools can help monitor changes in animal posture accurately and efficiently. To create a more robust system and eliminate varying lighting issues within a day including daytime/ nighttime differences, there is an advantage to using depth cameras over digital cameras. In this study, a computer vision …

Machine Learning-Based Disease Classification In Tomato (Solanum Lycopersicum) Plants = Klasifikasi Penyakit Berbasis Pembelajaran Mesin Pada Tanaman Tomat (Solanum Lycopersicum), Md Towfiqur Rahman, Sudipto Dhar Dipto, Israt Jahan June, Abdul Momin, Muhammad Rashed Al Mamun

Department of Agricultural and Biological Systems Engineering: Faculty Publications

English abstract

In Bangladesh, tomato cultivation faces significant challenges due to its susceptibility to various microorganisms, parasites, and bacterial infections. Typically, the early symptoms of these diseases first appear in roots and leaves, complicating timely detection. This study addresses the challenge of timely and accurate detection of diseases in tomato plants, crucial for effective plant protection management. Conventional manual inspection methods are time-consuming and subjective, resulting in delays in implementing necessary protection measures. Therefore, an image processing technique and machine learning algorithms were used for rapid and robust detection of diseases in tomato plant leaves, aiming to streamline the detection …

A Validation Of Wepp Water Quality Routines In Uniform And Nonuniform Agricultural Hillslopes, Ryan P. Mcgehee, Dennis C. Flanagan, Bernard A. Engel, John E. Gilley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Nutrients in agricultural runoff may cause offsite environmental impacts. The objective of this investigation was to examine nutrient transport by overland sheet flow on sites containing swine slurry. Data examined in this study was collected during field rainfall simulation tests conducted on cropland sites in southeast Nebraska, USA. Inflow was added to the top of experimental plots in four successive increments to simulate runoff rates occurring at greater downslope distances. Runoff rates on the experimental sites ranged from 2.3 to 21.2 L min 1 and maximum equivalent downslope distances varied from 5 to 108 m. Phosphorus (P) and nitrogen (N) …

Experimental Investigation On Properties Of Sugarcane Bagasse Fiberboard Manufactured With Different Techniques, Amany G. Micheal Prof., Marianne Nabil Girgis Ass. Prof., Omar Mohasen Khalaf Eng.

Architectural Engineering

Within an ongoing research project investigating the use of sugarcane bagasse (SCB) to develop different building materials, this article adopts an experimental approach to determine the effect of the manufacturing technique on proposed composite fiberboards. Two different prototypes of composite fiberboards are developed using (SCB) as a natural fiber, and different matrices are tested. The epoxy resin and polyester resin are used as the alternative matrices. Fiberboards are proposed to be used as cladding boards or as internal furnishing units material instead of using MDF or HDF that exploit the usage of natural soft or hard wood that is unrecyclable, …

Characterization Of Recent Wild Pig‑Vehicle Collisions In Georgia, Usa, Jeremiah L. Psiropoulos, Emily Howe, John J. Mayer, Sophie C. Mckee

United States Department of Agriculture Wildlife Services: Staff Publications

Vehicle collisions with wild pigs (Sus scrofa) are reported almost everywhere this species is found. However, this is one of the least studied and characterized forms of damage that these invasive animals cause in the United States (US). We analyzed 518 wild pig-vehicle collisions (WPVCs) that took place statewide in Georgia between 2015 and 2021. From that dataset, we analyzed several parameters in order to better understand and characterize these accidents on a scale that had previously not been done in the US. Wild pig-vehicle collisions were reported from 105 out of the 159 counties in Georgia, increasing …

Effects Of Crate Size On Mortality And Postural Behavior In Post-Farrowing Sows, Md Towfiqur Rahman, Tami M. Brown-Brandl, Gary A. Rohrer, Sudhendu R. Sharma, Yeyin Shi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Preweaning mortality (PWM) is a economic and animal well-being issue for swine producers in the US. On average, producers face about a 16% pre-weaning mortality. A significant portion of this, around 6%, is because sows accidentally overlay their piglets in farrowing crates. The posture behavior of sow plays a vital role in overlaying piglets and this behavior has a significant effect on farrowing crate dimensions. This study aims to explore the interplay between farrowing crate size, mortality rates, and postural behavior in post-farrowing sows. This study employed three farrowing stalls: Traditional (T), Expanded creep area (C), and Expanded sow area …

Closing Dichloramine Decomposition Nitrogen And Oxygen Mass Balances: Relative Importance Of End-Products From The Reactive Nitrogen Species Pathway, Huong T. Pham, David G. Wahman, Julian L. Fairey

Civil Engineering Faculty Publications and Presentations

In drinking water chloramination, monochloramine autodecomposition occurs in the presence of excess free ammonia through dichloramine, the decay of which was implicated in N-nitrosodimethylamine (NDMA) formation by (i) dichloramine hydrolysis to nitroxyl which reacts with itself to nitrous oxide (N₂O), (ii) nitroxyl reaction with dissolved oxygen (DO) to peroxynitrite or mono/dichloramine to nitrogen gas (N₂), and (iii) peroxynitrite reaction with total dimethylamine (TOTDMA) to NDMA or decomposition to nitrite/nitrate. Here, the yields of nitrogen and oxygen-containing end-products were quantified at pH 9 from NHCl₂ decomposition at 200, 400, or 800 μeq Cl₂·L …

An Optical Aptamer-Based Carbon Nanosensor For Cortisol Detection, Jessica Kretli Zanetti

Dissertations and Theses

Cortisol is a hormone related to numerous biological functions. This hormone is primarily associated with regulating the body’s stress response, including its effects on metabolism, immune function, and energy balance. Additionally, it plays a critical role in maintaining physiological homeostasis during periods of physical or psychological stress. Consequently, it is critical to monitor levels of this hormone to understand different vital processes it can impact. This work presents the development of an optical aptamer-based carbon nanosensor for the detection of cortisol. The sensor utilizes single-walled carbon nanotubes (SWCNTs) functionalized with a specific DNA aptamer to achieve high specificity and sensitivity. …

Using Mechanical Interventions To Enhance Drug Transport To Bone Tumors, Michelle Gelbs

Dissertations and Theses

Bone metastasis occurs when cancer cells spread from a primary site to a secondary location in bone. Current treatments for metastatic bone tumors typically involve systemic chemotherapy, bisphosphonates, radiotherapy, or surgery; however, due to morphological changes in the tumor microenvironment and elevated interstitial fluid pressure, tissue penetration of therapeutic agents is often more limited in cancerous tumors compared to normal tissue. Weight-bearing mechanical loading applied to bone, as well as other mechanical stimulations such as vibration, have been shown to cause loading-induced bone deformations that increase convective transport via interstitial fluid flow. We hypothesize that mechanical loading can increase the …

Optimizing Transcranial Focused Ultrasound: Calibration, Neuro Navigation, And Bold Fmri Evidence Of Neuromodulation, Amilcar J. Malave

Dissertations and Theses

This thesis presents the development, calibration, and initial implementation of transcranial focused ultrasound (tFUS) for neuromodulation in a human study. The primary objectives were to design a system capable of delivering precise and safe ultrasound stimulation to targeted brain regions and to conduct preliminary trials to assess its effects on brain activity. A custom calibration device was developed to ensure accurate measurement of acoustic pressure, addressing challenges such as voltage alterations caused by changes in system impedance. Additionally, a neuro-navigation tool was created to facilitate precise targeting of the right subgenual anterior cingulate cortex (right sgACC), enabling accurate placement of …

Implementation Of Acid/Gas Digestion Approach To Maximize Volatile Fatty Acids Production From Municipal Sludge And A Techno-Economic Feasibility Study, Aykut Sayin

Dissertations and Theses

Acid digestion, which is the first step of two-phase acid/gas anaerobic digestion process, was studied to identify the optimum operating conditions that would maximize the production of volatile fatty acids (VFAs). Batch and semi-continuous acid digesters were operated using combined primary sludge and waste-activated sludge as the feedstock from New York City (NYC)’s Wards Island water resource recovery facility (WRRF). Operating conditions tested in batch acid digesters for maximizing VFAs production included temperature (35-70°C), inoculum-to-substrate ratio (0.0-0.5 mgVS/mgVS), thickening (3-13%TS), and contact time (0-10 days), while simultaneously inhibition mechanisms on VFAs production were investigated. Once the optimum operating conditions for …

The Racial Disparities Of The Gamma-Secretase/Notch Pathway And Inhibition In Triple Negative Breast Cancer, Jobin Joseph

Dissertations and Theses

Triple Negative Breast Cancer (TNBC) continues to be a formidable challenge in oncology, characterized by its aggressive phenotype and limited treatment options. Alarming racial disparities in TNBC outcomes persist, with underrepresented populations experiencing disproportionately higher rates of mortality and poorer prognoses. This master's thesis aims to delve into the molecular intricacies that contribute to these disparities, with a specific focus on gamma secretase activity, a key regulator of cellular processes.

The research methodology encompasses a multi-faceted approach, amalgamating clinical data analysis with extensive in vitro and in vivo experimentation. The study employs cutting-edge molecular profiling techniques and gene expression analysis …

Alkaline Potassium Polyacrylate Electrolytes For Rechargeable Zinc-Manganese Dioxide Batteries, Jungsang Cho

Dissertations and Theses

Zinc (Zn)–manganese dioxide (MnO₂) rechargeable batteries have high specific theoretical capacity as well as being environmentally friendly, intrinsically safe and low-cost. Liquid electrolytes, such as potassium hydroxide (KOH), are historically used in these batteries; however, many failure mechanisms of the Zn–MnO₂ battery chemistry result from the use of liquid electrolytes, including the formation of electrochemically inactive compounds and shape change of Zn electrodes during charge and discharge cycling. This led to incorporation of an alkaline polymer electrolyte as an alternative to the liquid KOH electrolyte in Zn-MnO₂ rechargeable batteries, with the aim of making them non-spillable …

Actuator Optimization And Control Of Pediatric Knee Exoskeleton For Community-Based Mobility Assistance: More Lightweight, More Affordable, And More Stable, Sainan Zhang

Dissertations and Theses

Lightweight and smart exoskeletons offer the potential to improve mobility in children. State-of-the-art pediatric exoskeletons are typically clinic-based since they are either tethered or portable but cumbersome, and their design is often not optimized across a range of environments and users. Child growth progressively alters key exoskeleton design requirements, yet no prior work investigated actuator optimization to accommodate child growth. Moreover, exoskeletons typically use the torque sensor or spring mechanics as exteroceptive feedback, but these torque sensing methods not only lead to extra cost and mass but also cause a small range of stable controller gains.

To facilitate pediatric exoskeleton …

Mechanisms For Regenerative Intervertebral Disc Healing During Early Growth In Mice, Danielle N. D'Erminio

Dissertations and Theses

Annulus Fibrosus (AF) defects of intervertebral discs (IVDs) cause painful disability, for example when herniated tissue compresses against adjacent nerves. Discectomy, the current standard of treatment to address herniation, does not repair AF defects and can result in re-herniation and recurrent pain. As such there is a need for improved AF repair strategies. It has previously been established that neonatal mice functionally regenerate following severe AF injury providing a model of successful innate AF repair processes that contrasts the poor healing of adult mice. This dissertation focuses on identifying mechanisms of AF regenerative healing in neonatal mice subjected to severe …

Understand The Structure-Water-Responsive Relationship By Engineering Silk Materials, Yeojin Jung

Dissertations and Theses

Water-responsive (WR) materials mechanically swell and shrink in response to changes in relative humidity, and they have demonstrated the capability to exert higher actuation energy than conventional actuators and artificial muscles. As a result, WR materials have recently gained attention as potential high-energy actuating components for engineering applications. Despite the growing interest in this emerging category of WR materials, the fundamental WR mechanism of their significant performance is still not fully understood, limiting the ability to rationally design engineered systems. This dissertation presents three approaches for understanding the water-responsiveness of materials by engineering Bombyx (B.) mori silkworm silk protein. First, …