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Exploring The Impact Of Lake Browning On The Production Of Singlet Oxygen In Adirondack Surface Waters, Birdem Oz

Theses - ALL

Many inland lakes in temperate and boreal regions, including those in the Adirondack region of New York, are experiencing browning due to increased terrestrial inputs of colored dissolved organic matter (DOM). This phenomenon results from watershed recovery following declines in atmospheric acid deposition and shifts in precipitation patterns. Close coupling of changes in dissolved organic carbon levels and DOM quality in Adirondack lakes has important implications for ecosystem services, ranging from aquatic food web functioning to drinking water production. However, comparatively little is known about the potential effects of lake browning on carbon cycling and contaminant attenuation driven by the …

Multi-Scale Analysis Of The Structure-Mechanics Relationship Of Mycelium-Based Bio-Composites, Libin Yang

Dissertations - ALL

The construction industry faces significant challenges due to the environmental impact of traditional materials like steel and concrete, whose production is energy-intensive and contributes to environmental pollution. This issue is exacerbated by the growing global demand for construction materials, driven by an increasing population. Concurrently, the agricultural and wood industrial sectors produce vast byproducts, such as straw, husk, coir, sawdust, and wood chips. They are often considered as wastes and disposed of through methods like burning. Such a reckless treatment releases greenhouse gases that further lead to environmental degradation. Mycelium, the vegetative part of fungi, presents a natural, promising solution …

Fate, Composition And Formation Of Unknown N-Nitrosamines In Wastewater And Wastewater-Impacted Waters, Changcheng Pu

Dissertations - ALL

N-Nitrosamines form as byproducts during oxidative water treatment or occur as impurities in consumer and industrial products. N-Nitrosamines and their precursors pose significant concerns for water utilities exploiting wastewater-impacted water supplies, as many N-nitrosamines are potential human carcinogens and have genotoxicity. To date, research on N-nitrosamines in environmental engineering has primarily focused on the occurrence, sources, formation, and control of N-nitrosodimethylamine (NDMA) in the broad context of drinking water and wastewater treatment. However, NDMA and the few other N-nitrosamines of current interest only constitute a fraction of the larger pool of total N-nitrosamines (TONO) in source waters and wastewater, while …

Investigation Of Hydropathy Of Proteins, Lipid-Modified Proteins, And Self-Defensive Biomaterials, Jingjing Ji

Dissertations - ALL

Molecular dynamics (MD) simulations serve as crucial tools for unraveling the underlying physical principles governing the structure and functionality of biological macromolecules. Experimental techniques provide valuable results, but sometimes it is quite difficult to explain phenomena at atomic and molecular levels. MD simulations investigate physical motion of atoms and molecules over microsecond time scale and compute the equilibrium properties. In this work, I investigated the hydropathy of proteins, lipid-modified proteins, and self-defensive biomaterials with atomistic MD simulations and coarse-grained (CG) MD simulations. The method, Protocol for Assigning a Residue's Character on a Hydropathy (PARCH) scale, is developed to characterize and …

Homomeric Tight Junction Strand Assembly Of Claudin Proteins, Patrick Matthew Marsch

Dissertations - ALL

Tight junctions, a form of cell junction, are utilized throughout the animal kingdom in one way or another. Tasked with facilitating a critical blockade between toxins and vital tissues while also allowing small crucial molecules to pass, the tight junction provides an important line of defense against disease. To build this barrier, various proteins with self-assemble within a cell membrane to then zipper with another cell membrane. The strand-like architecture and size & charge selective pore channels created within the barrier are chiefly governed by the claudin family of proteins. Without claudins present, the tight junction cannot form, and toxic …

Engineering The Signal Resolution Of A Paper-Based Cell-Free Glutamine Biosensor With Genetic Engineering, Metabolic Engineering, And Process Optimization, Tyler J. Free, Joseph P. Talley, Chad D. Hyer, Catherine J. Millar, Joel S. Griffitts, Bradley Charles Bundy

Faculty Publications

Specialized cancer treatments have the potential to exploit glutamine dependence to increase patient survival rates. Glutamine diagnostics capable of tracking a patient’s response to treatment would enable a personalized treatment dosage to optimize the tradeoff between treatment success and dangerous side effects. Current clinical glutamine testing requires sophisticated and expensive lab-based tests, which are not broadly available on a frequent, individualized basis. To address the need for a low-cost, portable glutamine diagnostic, this work engineers a cell-free glutamine biosensor to overcome assay background and signal-to-noise limitations evident in previously reported studies. The findings from this work culminate in the development …

Stimuli-Responsive Glycopolymeric Nanoparticles To Boost Tumor Immunogenicity Through Macrophage-Targeted Therapy, Oluwaseyi Shofolawe-Bakare

Electronic Theses and Dissertations

Tumor-associated macrophages (TAMs) play a substantial role in sculpting the tumor microenvironment (TME) into an immunosuppressive bastion that undermines immune surveillance and enables tumor progression. They therefore represent a promising therapeutic target to make the TME more conducive for anti-tumor immunity and greatly improve treatment outcomes in cancers that are highly resistant to conventional forms of therapy. Several medications have been developed to modulate the activities of TAMs, showing tremendous potential, but these medications lack specificity for TAMs making their use susceptible to adverse side effects that could outweigh prospective benefits. Sophisticated delivery vehicles that can preferentially deliver these medications …

An Attention Lstm U-Net Model For Drosophila Melanogaster Heart Tube Segmentation, Xiangping Ouyang

McKelvey School of Engineering Graduate Student Theses & Dissertations

Machine learning is commonly used in biomedical image analysis, as it allows automated image segmentation and identification that minimizes the need for tedious human involvement. Drosophila melanogaster is often used as a cardiac disease model, where optical coherence microscopy (OCM) is used to image and analyze its beating dynamics. As OCM often generates a large volume of images, automated image segmentation is necessary to quantify the heart beating efficiently. Our most recent heart segmentation model, FlyNet 2.0+, is a fully convolutional LSTM U-Net model. However, the performance of the model diminishes in the presence of artifacts, such as image reflection …

Investigating The Effects Of Suspended Algal Content, Dissolved Oxygen, And Temperature On A High-Frequency Acoustic Attenuation System In A Controlled Laboratory Environment, Noah Watson

Honors Theses

The Single Frequency Acoustic Attenuation System (SFAAS) has been developed at the National Center for Physical Acoustics (NCPA). This system uses acoustic methods to monitor fine suspended sediment concentration in streams. Site-specific calibrations are necessary for this system, and previous research has indicated that algal concentration or dissolved oxygen concentration of water may affect collected data. Temperature, dissolved oxygen concentration, and fluorescence, used as a proxy for algal concentration, were monitored during this system’s use, and calibrations were derived to account for the effect of each of these variables on acoustic measurements. It has been shown that temperature has a …

Effect Of Treated/Untreated Recycled Aggregate Concrete: Structural Behavior Of Rc Beams, Ayman Abdo, Ayman El-Zohairy, Yasser Alashker, Mohamed Abd El-Aziz Badran Mohamed

Faculty Publications

Using recycled concrete aggregates from construction and demolition wastes on structural concrete is a sustainable solution to reduce the consumption of natural resources and the detrimental effects of concrete production on the environment. This paper has collected much data from the literature to study fresh, mechanical properties and durability of concrete made of treated/untreated recycled aggregate (RA). Furthermore, the flexural and shear behavior of recycled aggregate concrete (RAC) beams was studied. This study discussed the distinctions and similarities between reinforced RAC beams and reinforced natural aggregate concrete (NAC) beams. The results of this review’s analysis clearly show that reinforced RAC …

Co-Designing Situated Displays For Family Co-Regulation With Adhd Children, Lucas M. Silva, Franceli L. Cibrian, Clarisse Bonang, Arpita Bhattacharya, Aehong Min, Elissa M. Monteiro, Jesus A. Beltran, Sabrina E. B. Schuck, Kimberley D. Lakes, Gillian R. Hayes, Daniel A. Epstein

Engineering Faculty Articles and Research

Family informatics often uses shared data dashboards to promote awareness of each other’s health-related behaviors. However, these interfaces often stop short of providing families with needed guidance around how to improve family functioning and health behaviors. We consider the needs of family co-regulation with ADHD children to understand how in-home displays can support family well-being. We conducted three co-design sessions with each of eight families with ADHD children who had used a smartwatch for self-tracking. Results indicate that situated displays could nudge families to jointly use their data for learning and skill-building. Accommodating individual needs and preferences when family members …

The Effects Of Additive Manufacturing On Canine Hind Limb Repairment Surgery, Annabel Keers

Honors Theses

The intention of this project is to explore some of the ways that additive manufacturing is affecting veterinary medicine. I will specifically be delving into canine hind limb repairment surgeries, such as cranial cruciate ligament reconstruction. 3D printed prototypes have been utilized in the human medical field for quite a while, and it has inspired such technology to expand into the world of veterinary medicine. Such emerging research is expected to take-off as the pet market grows in size and level of care for the health of our animals.

I took special interest in this field as I will be …

Imaging Of Structural Timber Based On In Situ Radar And Ultrasonic Wave Measurements: A Review Of The State-Of-The-Art, Narges Pahnabi, Thomas Schumacher, Arijit Sinha

Civil and Environmental Engineering Faculty Publications and Presentations

With the rapidly growing interest in using structural timber, a need exists to inspect and assess these structures using non-destructive testing (NDT). This review article summarizes NDT methods for wood inspection. After an overview of the most important NDT methods currently used, a detailed review of Ground Penetrating Radar (GPR) and Ultrasonic Testing (UST) is presented. These two techniques can be applied in situ and produce useful visual representations for quantitative assessments and damage detection. With its commercial availability and portability, GPR can help rapidly identify critical features such as moisture, voids, and metal connectors in wood structures. UST, which …

Analysis Of Modeled 3d Solar Magnetic Field During 30 X/M-Class Solar Flares, Seth H. Garland, Vasyl B. Yurchyshyn, Robert D. Loper, Benjamin F. Akers, Daniel J. Emmons Ii

Faculty Publications

Using non-linear force free field (NLFFF) extrapolation, 3D magnetic fields were modeled from the 12-min cadence Solar Dynamics Observatory Helioseismic and Magnetic Imager (HMI) photospheric vector magnetograms, spanning a time period of 1 hour before through 1 hour after the start of 18 X-class and 12 M-class solar flares. Several magnetic field parameters were calculated from the modeled fields directly, as well as from the power spectrum of surface maps generated by summing the fields along the vertical axis, for two different regions: areas with photospheric |B_z|≥ 300 G (active region—AR) and areas above the photosphere with the …

Development Of A Rapid Bacterial Quantification Method Based On Droplet Microfluidics, Shawn Bliss

Electronic Theses and Dissertations

The capacity to identify bacteria quickly and accurately is critical for applications such as medical diagnosis, environmental monitoring, and food safety. For instance, sepsis diagnosis requires blood cultures that take as long as 1-5 days, preventing timely intervention and increasing mortality rate. Here, we propose the joint use of microfluidics and a machine learning algorithm for rapid bacterial cell capture and quantification. The combination of high-throughput droplet microfluidics and a support vector machine (SVM) enables analysis and quantification of bacterial samples within as short as 4 hours. We have performed successful encapsulation of pathogenic bacteria such as E. coli, …

Valorisation Of Water Hyacinth For Different Engineering Applications, Nessren Farrag, Zeinab Elbhnsawi, Omar Aboelazayem

Chemical Engineering

Nature has many elements that can be beneficial or harmful. One of these elements is water hyacinth (WH) which is an aquatic weed found in water bodies such as rivers and lakes and it has an invasive nature due to its rapid growth rate. On the contrary, water hyacinth, despite its classification as an invasive plant species, possesses a multitude of industrial and engineering applications, demonstrating substantial potential for valorisation into various products. Its versatile attributes and widespread availability render water hyacinth a viable resource for deployment across several sectors, including wastewater treatment, bioenergy generation, bioremediation, and sustainable agriculture. The …

Silver Assisted Pani-Rgo: A Cutting-Edge Approach To Supercapacitor Applications, Iman El-Mahallawi, Ayat Abdelsalam, Ahmed Selim, Mahmoud Tash, Waleed Khalifa

Mechanical Engineering

In this research, highly efficient and low cost synthesis approach was applied to produce three distinct electrode materials: reduced graphene oxide (RGO), silver nanoparticles incorporated with RGO, and polyaniline (PANI) in conjunction with RGO decorated with silver nanostructures (RGO.Ag). These materials were subsequently integrated into a composite electrode material as PANI RGO.Ag, designed specifically for deployment in symmetric supercapacitor applications. A comprehensive characterization process was executed employing a range of material analysis techniques, including X-Ray Diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDX).

Calcium Analysis Of Intercellular Communications Between Alpha Cells And Delta Cells Across The Pancreatic Islet, Shichao Gao

McKelvey School of Engineering Graduate Student Theses & Dissertations

The intricate communication between different cell types within pancreatic islets plays a crucial role in regulating glucose homeostasis. Among these cellular interactions, the interplay between α cells (responsible for glucagon secretion) and δ cells (secreting somatostatin) is particularly intriguing due to the inhibitory effects of somatostatin on glucagon release. Understanding the intercellular interactions between α and δ cells may provide insights into the mechanisms underlying the coordinated regulation of blood glucose levels. In this study, we employed a multi-modal approach combining confocal imaging, image analysis, and correlation network analysis to investigate the spatial relationships and potential functional connections between α …

Pre-Incisional And Multiple Intradermal Injection Of N-Acetylcysteine Slightly Improves Incisional Wound Healing In An Animal Model, Wictor Pascal, Antoni Smolinski, Mateuz Gotowiec, Marta Wojtkiewicz, Albert Stachura, Kaspar Pelka, Michal Kopka, Kyle P. Quinn, Alan E. Woessner, Dariusz Grzelecki, Pavel Wlodarski

Biomedical Engineering Faculty Publications and Presentations

The objective of this study was to investigate if delivering multiple doses of N-acetylcysteine (NAC) post-surgery in addition to pre-incisional administration significantly impacts the wound healing process in a rat model. Full-thickness skin incisions were carried out on the dorsum of 24 Sprague-Dawley rats in six locations. Fifteen minutes prior to the incision, half of the sites were treated with a control solution, with the wounds on the contralateral side treated with solutions containing 0.015%, 0.03% and 0.045% of NAC. In the case of the NAC treated group, further injections were given every 8 h for three days. On days …

Acoustofluidic Scanning Fluorescence Nanoscopy With A Large Field Of View, Geonsoo Jin, Neil Upreti, Joseph Rich, Jianping Xia, Chenglong Zhao, Tony Jun Huang

Faculty Publications

Large-field nanoscale fluorescence imaging is invaluable for many applications, such as imaging subcellular structures, visualizing protein interactions, and high-resolution tissue imaging. Unfortunately, conventional fluorescence microscopy requires a trade-off between resolution and field of view due to the nature of the optics used to form the image. To overcome this barrier, we developed an acoustofluidic scanning fluorescence nanoscope that simultaneously achieves superior resolution, a large field of view, and strong fluorescent signals. The acoustofluidic scanning fluorescence nanoscope utilizes the superresolution capabilities of microspheres that are controlled by a programmable acoustofluidic device for rapid fluorescence enhancement and imaging. The acoustofluidic scanning fluorescence …

Spin Coating Multilayer Polymer Films To Improve The Consistency And Longevity Of Interval Drug Release, Katherine Nevils

Honors Theses

Treatment-resistant depression (TRD) is a clinical diagnosis for patients suffering from major depressive disorder (MDD). An inadequate response to the dosage or duration of antidepressant medication can lead to the diagnosis of TRD. This diagnosis is common in up to 50% of patients with MDD following treatment. Microdosing of classical psychedelics such as lysergic acid diethylamide (LSD) has been shown to have positive cognitive and therapeutic effects on patients with TRD. However, issues of patient compliance to the complex regimen and abuse/diversion of the schedule I controlled substances remain unresolved. The goal of this project is to create an implantable, …

Creating A Collapsible Sling Lift Capable Of Transporting Patients Between Various Locations, Cameron Delaney Hillman, Macey Jewell Ross, Lydia Noel Miller

Honors Theses

As Senior Biomedical Engineering students at the University of Mississippi, we, Lainey Hillman, Lydia Miller, and Macey Ross, are required to complete a final senior capstone for our department. This project aims to provide a solution to a current unmet clinical need in the healthcare industry, thus our team sought to improve the lack of transportability of sling lifts. The current sling lifts on the market display numerous issues: heavy, bulky, use confined to certain areas, and use specific for only one type of transportation. To combat these problems, we designed a sling lift that was lightweight, collapsible, safe, and …

Comparative Life Cycle Assessment Of Hydrogen Production Via Various Pv-Assisted Electrochemical Water Splitting Techniques, Achyuth Ravilla, Ilke Celik

Student Research Symposium

Photoelectrochemical (PEC) and photovoltaic-electrochemical (PV-EC) water-splitting technologies have emerged as cost-effective options for large-scale green hydrogen production in industrial applications. Solar to hydrogen (STH) efficiencies of these technologies have reached up to 20% and several pathways have been explored to drive down the cost of hydrogen using these technologies to less than $2/kg. However, the environmental impact assessment of these technologies for industry-scale deployment has not been explored in previous studies. This study assesses the environmental impacts of PEC and PV-EC technologies by conducting a cradle-to-gate life cycle assessment. The functional unit considered for this assessment is 1 kg of …

Pulse Modulation In Braided Pneumatic Actuators Mimics Contractile Behavior Of Biological Muscles, Mohamad Elzein

Student Research Symposium

Advancements in robotics and bioengineering aim to emulate biological muscle systems through robotic actuators, blending mechanical strength with biological adaptability. A lesser-explored aspect is mimicking the pulse-like control characteristic of biological muscles, which contract in response to action potentials from motoneurons, with muscle contractile force relying heavily on the timing between these potentials. This study explores the effect of pulse lengths and the gaps between pulses on braided pneumatic actuators (BPAs), which mimic the nonlinearity and dynamic response of biological muscles. It hypothesizes that artificial muscles utilizing pulse-based control will exhibit a similar force dependency on the intervals between pulses …

Self-Balancing Robot Leg, Ben Bolen

Student Research Symposium

Research in the Agile and Adaptive Robotics Lab involves the creation of biomimetic robots. To this end, we developed a self-balancing robot leg actuated with braided pneumatic actuators (BPAs)—a type of pneumatic artificial muscle. These BPAs, akin to human muscles, exhibit properties such as high strength-to-weight ratio and tunable passive stiffness. An Inertial Measurement Unit (IMU) was placed on top of the tibia for feedback and the tibia and foot were connected with a hinge joint. The orientation of the ankle joint was controlled with an Arduino microcontroller sending commands to the proportional pressure valves supplying the BPAs. Leg balance …

Deriving Analytical Design Constraints For Absolute & Relative Encoding Schemes In Functional Subnetworks, Cody W. Scharzenberger-Braet, Alexander Hunt

Student Research Symposium

As neural networks have become prolific solutions to modern problems, there has been a congruent rise in the popularity of the numerical machine learning techniques used to design them. While they are highly generalizable, numerical methods tend to produce networks that act as inscrutable “black boxes,” making it difficult to interpret their behavior. One solution to the problem of network transparency is to use analytical techniques, but these methods are underdeveloped compared to their numerical alternatives. In order to enhance the viability of analytical techniques, this work extends previous efforts to quantify the impact that non-spiking neural encoding schemes have …

Revitalizing Turtle Creek Park, Michael Hardyway, Ethan Harm, Abbey Jacoby, Casey Stephenson

Final Reports in ENST 411: Environmental Community Projects

We current ENST 411 students, Abbey Jacoby, Michael Hardyway, Ethan Harm, and Casey Stephenson have chosen to work with Jim Knight, East Buffalo Township, the Merrill Linn Land and Waterways Conservancy, and many others in an attempt to revitalize Turtle Creek Park for a plethora of reasons. Three of us are majoring in biology, and two are majoring in environmental science, which makes much of the information and techniques relevant in Turtle Creek applicable to our courses of study. This project included heavy hands on work which allowed us students to leave a memorable and impactful influence on the Lewisburg …

Effects Of Magnetite Particle Morphology On Adsorption Of Copper Ions From Aqueous Solutions, Alisa Hashley

Graduate Theses & Non-Theses

Adsorptive processes can be used for metal contaminant removal. This work addresses magnetite, a magnetic iron oxide, as the adsorbent for use in an adsorptive removal system, known as the continuous flow material recovery system (CFMR), developed by Leitzke et al. [1]. Though the system is effective in removing contaminants from aqueous solution, efforts to further improve efficiency are being made. One way to improve the efficiency of the CFMR is to analyze the magnetite particles being used and investigate how the particle properties effect adsorption. The author’s research is presented and discussed here to describe the effects magnetite particle …

Liquid-Phase Adsorption Of Dimethyl Methylphosphonate On Poly(Ether Imide) And Its Metal Hydroxide-Integrated Composites, Sukanta K. Mondal, Peter O. Aina, Ali A. Rownaghi, Fateme Rezaei

Chemical and Biochemical Engineering Faculty Research & Creative Works

Understanding the Adsorption Behavior of Dimethyl Methylphosphonate (DMMP), a Key Simulant for Some Nerve Agents, is Crucial for Developing Effective Protective Measures and Ensuring Environmental Safety Against Harmful Chemical Warfare Agents. This Study Systematically Explores the Liquid-Phase Adsorption of DMMP over Branched Poly (Ether Imide) (Ultem) and its Ce (OH)4/Zr (OH)4 Metal Hydroxide Composites. Utilizing UV-Visible (UV-Vis) Spectroscopy, the Adsorption Process Was Monitored over Time to Analyze the Interplay between Various Ultem-Based Composites and DMMP Molecules. Nonreactive Ultem Materials Were Experimentally Identified as Suitable Adsorbents for Liquid-Phase DMMP Capture, Providing a Novel Avenue for Protective Filtration Technologies. It Was Found …

Evaluating Neuroimaging Modalities In The A/T/N Framework: Single And Combined Fdg-Pet And T1-Weighted Mri For Alzheimer’S Diagnosis, Peiwang Liu

McKelvey School of Engineering Graduate Student Theses & Dissertations

With the escalating prevalence of dementia, particularly Alzheimer's Disease (AD), the need for early and precise diagnostic techniques is rising. This study delves into the comparative efficacy of Fluorodeoxyglucose Positron Emission Tomography (FDG-PET) and T1-weighted Magnetic Resonance Imaging (MRI) in diagnosing AD, where the integration of multimodal models is becoming a trend. Leveraging data from the Alzheimer's Disease Neuroimaging Initiative (ADNI), we employed linear Support Vector Machines (SVM) to assess the diagnostic potential of these modalities, both individually and in combination, within the AD continuum. Our analysis, under the A/T/N framework's 'N' category, reveals that FDG-PET consistently outperforms T1w-MRI across …