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Response To Replication Stress And Maintenance Of Genome Stability By Wrn, The Werner Syndrome Protein, David K. Orren, Amrita Machwe

Markey Cancer Center Faculty Publications

Werner syndrome (WS) is an autosomal recessive disease caused by loss of function of WRN. WS is a segmental progeroid disease and shows early onset or increased frequency of many characteristics of normal aging. WRN possesses helicase, annealing, strand exchange, and exonuclease activities and acts on a variety of DNA substrates, even complex replication and re- combination intermediates. Here, we review the genetics, biochemistry, and probably physiological functions of the WRN protein. Although its precise role is unclear, evidence suggests WRN plays a role in pathways that respond to replication stress and maintain genome stability particularly in telomeric regions.

Enhancing Water Sustainability In North Africa: Literature Review And Synthesis Of Current Knowledge Gaps In Sudan, Osman M. A. Adam, Aaron R. Mittelstet, Derek M. Heeren, Troy E. Gilmore

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This study delves into the critical role of groundwater in addressing global water challenges, with a focus on the Nubian Sandstone Aquifer System (NSAS) in North Africa. Groundwater constitutes a source of potable water, irrigation, and industrial use, especially in arid regions where surface water is limited. We analyzed the status of water quantity, withdrawals, recharge, and geological characteristics in the NSAS, specifically in Sudan, Egypt, Libya, and Chad. Though the NSAS is largely an untapped resource, we evaluated various scenarios to determine the quantity of cropland that can be sustainably irrigated. The NSAS is located in an arid region, …

Assessing Soil Properties For Optimized Irrigation Development In Sudan, Northern Africa, Suhib Hamid, Derek M. Heeren, Aaron R. Mittelstet, Saleh Taghvaeian, Randall S. Ritzema

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Sustainable irrigation methods maximize agricultural productivity in Northern African countries like Sudan. This project evaluates soil properties to develop optimized irrigation strategies for Sudan's arid and semi-arid climate. Assessing soil characteristics such as texture, infiltration rates, and nutrient content informs the selection of modern irrigation systems. Data from 3,192 locations, collected using GPS and ring infiltrometers for infiltration rates, alongside laboratory analyses for soil properties, were employed. A ranking system determined suitable irrigation systems for specific soil classes, considering factors like slope, wind, crop tolerance, available water capacity (AWC), drainage, germination, capital cost, labor cost, maintenance, and evapotranspiration (ET). The …

Beyond The Waterfall: A Review Of Project Management Methodologies In Stem, Yessenia Henriquez, Brendan O'Toole

Entertainment Engineering and Design Student Research

Project management has been exercised from the early stages of history to concurrent practices happening today. It has evolved from the early stages of the Gantt chart and five primary principles to well-renowned project management methodologies such as Waterfall and Agile. This literature review focuses on comprehending four project management methodologies (Waterfall, Agile, Kanban, and Scrum), learning their criteria and frameworks, and learning about their application in a specific STEM environment when applicable. This work reviews the recent research literature pertaining to these four methods. An overview of project management certificates and guidelines is covered as well. Waterfall is known …

Enhancement Of Polytetrafluoroethylene-Polyvinyl Alcohol Nanofibers Triboelectric Effect With Carboxylic Functionalized Multiwalled Carbon Nanotubes Coating, Fernando Viesca

Theses and Dissertations

In this study, we fabricated a TENG using Forcespinning® (FS) Polytetrafluoroethylene microfibers (FS-PTFE-MFs) and Electrospun (ES) Polyvinyl alcohol nanofibers (ES-PVA-NFs). The FS-PTFE-MFs were physically linked with functionalized carboxylic multiwall carbon nanotubes (MWCNTs-COOH) employing water dispersions. MWCNTs-COOH were dispersed using three different surfactants: Sodium Dodecyl Sulfate (SDS), Sodium dodecylbenzenesulfonate (SDBS), and Triton X-100 (TX-100). Coated FS-PTFE-MFs were then characterized using a field emission scanning electron microscope (FESEM) with an energy dispersive X-ray spectroscopy (EDS) attachment and Fourier-transform infrared spectroscopy (FTIR). While no significant difference in performance was observed with different surfactants, electrical results of the TENG recorded a notable enhancement of …

Dynamic Metabolic Flux Analysis Amidst Data Variability: Sphingolipid Biosynthesis Case Study In Arabidopsis Thaliana Cell Cultures, Abraham Boluwatife Osinuga

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Sphingolipids are pivotal for plant development and stress responses. Growing interest has been directed towards fully comprehending the regulatory mechanisms of the sphingolipid pathway. In this study, we explore its de novo biosynthesis and homeostasis in Arabidopsis thaliana cell cultures, shedding light on fundamental metabolic mechanisms. Employing ¹⁵N isotope labeling and quantitative dynamic modeling approach, we obtained data with notable variations and developed a regularized and constraint-based Dynamic Metabolic Flux Analysis (r-DMFA) framework to predict metabolic shifts due to enzymatic changes. Our analysis revealed key enzymes such as sphingoid-base hydroxylase (SBH) and long-chain-base kinase …

Zero Dynamics Attacks On Unknown Bilinear Systems: Vulnerability And Detection, Mohammad Aminul Haq

Electrical & Computer Engineering Theses & Dissertations

Critical infrastructure requires a safe and secure operating environment because of its significant impact on society. Its large-scale size and distributed sensors and actuators make it vulnerable to cyber-physical attacks. A zero dynamics attack is a type of cyber-physical attack where an adversary keeps the output of the target constant (classically zero), while forcing some of the internal states to deviate from their nominal values. Most of the existing work in the literature assumes the system dynamics are linear and available to an adversary. The first goal of this dissertation is to show that an adversary can successfully execute a …

Process Modeling Of Composites For Automated Fiber Placement, Von Clyde Jamora

Mechanical & Aerospace Engineering Theses & Dissertations

Fiber-reinforced composites are available in various material systems, each requiring specific manufacturing techniques to exploit their unique properties fully. Systems such as woven fabrics and preimpregnated fibers employ distinctive methods to harness these properties effectively. For instance, non-crimp fabrics (NCF) use high-pressure resin transfer molding and prepregs, like IM7-8552, use manufacturing method called automated fiber placement (AFP). However, these manufacturing processes often introduce deposition features that can evolve into defects. Their occurrence and impact on morphology must be predicted using experimentally informed finite element models. The research began with compaction experiments on NCF fabric, leading to the development of a …

Surface Acoustic Waves-Based Point-Of-Care Biosensor, Debdyuti Mandal

Theses and Dissertations

A biosensor is an analytical tool consisting of biologically active material used in close conjunction with a device that converts a biochemical signal into a quantifiable electrical signal. The early detection of the disease biomarkers can enhance the increased curability of the disease, reduce healthcare costs, and finally, improve the quality of life for patients. Thus, for the following purpose, there is always a need for an effective biosensor as a diagnostic device. The biosensor typically consists of an actuator, a bio-recognition component, and a transducer that converts captured signals into a meaningful display. The bio-recognition component uses biomolecules to …

Enabling Novel Nanopore Sensing Approaches For Personalized Medicine Through Chemical Modifications To Analytes, Zehui Zhang

Theses and Dissertations

Nanopore sensors have garnered significant attention recently, not only for their groundbreaking advancements in single-molecule DNA sequencing but also for their diverse applications in stochastic sensing, single-molecule chemistry, and protein unfolding. The interaction of single molecules with the nanopore sensing interface leads to characteristic ionic current modulations, reflecting the composition, charge, distribution, structure, and sequence of the translocating molecule. With the electrical resistive pulse nanopore sensing, its single-molecule sensitivity and specificity have opened doors to numerous potential applications in personalized medicine. I will discuss recent advancements in the sensitivity and specificity of personalized medicine using nanopore sensors, focusing on proteomic …

Additively Manufactured Flexible Piezoelectric Wave-Based Multifunctional Sensor, Rishikesh Srinivasaraghavan Govindarajan

Doctoral Dissertations and Master's Theses

The demand for acoustic wave-based sensors has rapidly increased in the aerospace, chemical, gas, and biological fields due to their versatility in sensing measurands. This study aims to develop a flexible piezoelectric sensor exhibiting enhanced piezoelectric properties using additive manufacturing techniques that can detect mechanical strains and gas or volatile organic compounds (VOCs) by incorporating functional material into the sensing layer. This research explores piezoelectric substrate fabrication through diverse additive manufacturing techniques, including new material development made of polymer/nano-fillers with electrodes that are filled using different printing techniques. Notably, the design of the interdigital transducer (IDT) in the piezoelectric sensor …

Patient-Specific Hemodynamic Modeling For Optimizing Left Ventricular Assist Device And Right Heart Health, Holly Felice Grant

Theses and Dissertations

LVAD speed optimization is essential for efficient LVAD therapy and to minimize complications. However, the ideal LVAD speed is unknown and must be tailored for each patient. The interplay between the LVAD, systemic, and pulmonary circulations is critical and should be considered during optimization. A computational hemodynamic model that can be tailored for each patient may provide clinicians with useful guidance and reduce the need for repeated invasive studies. This study sought to customize and evaluate a patient-specific LVAD hemodynamic optimization model.

We conducted a retrospective analysis on 25 patients implanted with HM3 and who underwent right heart catheterization (RHC) …

Experimental Investigation Of The Self-Healing Potential Of Bacteria For Sustainable Concrete Structures Phase 2, Andrea Calabrese, Pitiporn Asvapathanagul, Nisarg N. Patel, Nanubala Dhruvan, Austin Adams, Michael Hernandez, Douglas S. Lopez-Cruz

Mineta Transportation Institute

Concrete is a critical component of so much of the modern construction industry. This material, well known for its versatility, robustness, longevity, and strength, is well-suited for a wide range of structural applications. Nonetheless, the widespread occurrence of cracks in concrete structures, primarily attributed to its limited tensile strength, shrinkage, and overstain, imposes a considerable economic and environmental challenge when it comes to retrofitting these fissures. This study tackles this problem by harnessing bacteria tolerant to high alkaline conditions to enable Microbially Induced Calcium Carbonate Precipitation (MICP) for the self-repair of concrete. This is achieved through an external application method, …

Innovative 4d Printing Of Biomimetic Collagen-Tcp Scaffolds For Bone Regeneration, Karly Morgan Liebendorfer

Theses and Dissertations

Bone tissue engineering (BTE) is a promising alternative approach to autografts and allografts when treating various bone diseases and disorders. BTE enables the fabrication of biocompatible, biomimetic, and osteo-stimulating constructs for the repair and regeneration of damaged bone tissue. The native bone environment primarily consists of anisotropic type 1 collagen (organic) and hydroxyapatite nanocrystals (inorganic). Composite biomimetic scaffolds fabricated by combining collagen with bioceramics have shown immense promise for BTE applications. Prior work has reported on different biofabrication methods to synthesize collagen-based composite scaffolds for use in BTE applications. However, to date, a customizable 3D composite scaffold that resembles the …

T. Boudieri Extract Potentiates The Effects Of Capecitabine Treatment In Human Colon Cancer Cells, Katia Sawaya, Mahmoud Khalil, Ghada Khawaja

BAU Journal - Science and Technology

Resistance to chemotherapy remains a major challenge for colorectal cancer patients worldwide, hence the persistent need to uncover alternative treatments as well as new adjunct therapies in the fight against cancer. Natural extracts constitute an excellent source of bioactive substances that have a promising potential in that regard, with minimal negative side effects. We have previously demonstrated that both the ethanolic as well as the water extracts of Terfezia boudieri, a black desert truffle abundant in and part of the cuisine of the Mediterranean region, have significant antiproliferative effects against colon cancer cells. Our aim in this study was …

Artificial Intelligence For Authentication Through Mental Profiling Technique, Nawaf Mazloum, Christine Abou-Saleh, Hassan Mazloum, Charles Anosike

BAU Journal - Science and Technology

A new approach to user authentication using mental profiling saw light, which is a technique that involves measuring an individual's cognitive abilities and psychological traits to create a unique profile, using Artificial Intelligence techniques. These profiles can then be used to identify users securely and efficiently, even in the presence of sophisticated attacks, by harnessing AI-driven systems for mental profile creation and user authentication.

After reviewing the existing literature on authentication and mental profiling, it presents a new mental profiling test that is specifically designed for authentication purposes. The test was evaluated on a sample of 100 users, and results …

An In – Depth Analysis Of Natural Fibers :A Comprehensive Review Of Properties And Performance Characteristics, Abbas Chour, Jamal Khatib

BAU Journal - Science and Technology

The purpose of this research is to investigate the usage of natural fibers in plant-based natural fiber-reinforced cement-based composites for sustainable construction materials. It summarizes research on various fiber kinds, their properties, and their impact on cement-based products, with a focus on renewable possibilities such as flax, jute, ramie, hemp, cotton, sisal, and kenaf. Due to their hydrophilic nature, plant fibers and polymers present compatibility concerns and moisture damage when combined. The paper examines ways for improving durability, such as physical and chemical treatments to improve aging resistance, optimizing binder components, and modifying curing regimens to reduce alkalinity in the …

Final Report - Autonomous Ultrasonic Thickness Measurement By A Magnet-Wheeled Robot, Yang Wang, Hung Manh La, Yu Otsuki, Son Thanh Nguyen

Project SN-6

This project develops a compact ultrasonic thickness sensing device that is integrated with a steel-climbing bicycle robot for autonomous thickness measurement of steel bridge members. With wireless communication capability, the ultrasonic sensing device can perform high-voltage pulse excitation, filtering and amplification of the received ultrasonic signal, and high-speed analog-to-digital conversions (up to 80 MHz). After successful validations of the device, it is integrated into a bicycle-like robot with two magnetic wheels controlled by two independent steering actuators that navigates on various steel surfaces (flat, curved, rough, concave, and convex). Along with the wireless ultrasonic sensing device, the robot carries a …

Advanced Eye Movement Tracking Using Computer Vision For Physically Disabled Patients, Anitha V R, Nisha Ponnambalam, Impana Bharadwaj, Rajat Mundhra

Manipal Journal of Science and Technology

The paper introduces an efficient mobility assistance system to empower individuals with moderate to severe physical disabilities and chronic disorders such as Quadriplegia (Paralysis of four limbs) and Amyotrophic Lateral Sclerosis, utilizing advanced technology. The project focuses on developing an affordable eye-controlled wheelchair using Raspberry Pi, Pi camera, DC motors, proximity sensor, and computer vision technology. The core innovation involves integrating a Pi camera onto the wheelchair to obtain a live acquisition feed of the patient’s eye. This captured eye movement serves as a control input to the Raspberry Pi board. Advanced computer vision algorithms analyze and translate the patient’s …

Development Of Poly-Vinyl Alcohol (Pva) And Moringa Oleifera-Based Nanofibre Patches For Wound Healing Using An Indigenous Electrospinning Setup, Suvarna Ghosh, Merla Sudha, Rakshith Prajwal R S, Sameer A Nadaf, Shreya R S, Pronama Biswas, Belaguppa Manjunath Ashwin Desai

Manipal Journal of Science and Technology

Wound healing is a crucial process that underlines the body’s ability to recover and restore damaged tissues. Conventional cotton-based dressings act as a physical barrier between the environment and open wound and are not actively involved in wound healing. This type of dressing can lead to maceration of scab, and infection and can cause pain upon removal. Functionalized nanofibre patches that can accelerate wound healing are being researched worldwide to overcome these challenges. Electrospinning has emerged as the most promising technique for engineering nanofibres for diverse applications. In this study, the wound-healing potential of PVA nanofibres functionalized with different concentrations …

Optimizing The Expression Of Polyethylene Terephtalate Hydrolase-Encoding Synthetic Gene In Escherichia Coli Arctic Express (De3), Jocelyn Nataniel, Maria Ulfah, Dini Achnafani, Niknik Nurhayati, Gabriela Christy Sabbathini, Sri Rezeki Wulandari, Abinawanto Abinawanto, Is Helianti

Makara Journal of Science

The waste of polyethylene terephthalate (PET) plastic waste in Indonesia is a pressing concern due to its slow degradation and potential environmental damage. One promising solution is to utilize polyethylene terephthalate hydrolase from Ideonella sakaiensis (IsPETase), an enzyme that specifically degrades PET. However, inducing the expression of IsPETase synthetic gene in Escherichia coli BL21 (DE3) has been challenging because much of it remains insoluble. This study aimed to express IsPETase in E. coli Arctic Express (DE3) and optimize the conditions to enhance its production. First, pET22b(+)pelB-IsPETase was inserted into E. coli Arctic Express (DE3). The …

Title: List Of 132 Papers Citing One Or More Skin Lesion Image Datasets, Neda Alipour, Ted Butke, Jane Courtney

Other resources

This document is provided as a supplementary digital resource to accompany the article Skin Type Diversity in Skin Lesion Datasets: A Review. The following list contains 132 papers that were identified during a systematic review of papers that use one or more skin lesion image datasets. A partial list of these papers appears in Table 1 of the aforementioned article. The full list is provided here for reference.

Control And Management Of The Balance Of Active And Reactive Power In A Power Supply System With A Solar Power Plant, Narzullo Nuriddinovich Mirzoyev, Siroj Sobirovich Nurov

Chemical Technology, Control and Management

This article discusses the development of a measurement and control system for monitoring and managing the balance of active and reactive power at power plants based on “Green” energy sources. Monitoring and management of the balance of active and reactive power based on a measurement and control system, calculated expressions are developed to solve the problem of reactive power compensation when transmitting electricity at power plants based on “green” energy sources. Reactive power is calculated based on measured active power, and combined reactive power sources generate the reactive power required by the network based on control signals. Schemes for connecting …

Manufacturing Of Aligned Electrospun Nanofiber Yarns For Their Enhanced Mechanical And Piezoelectric Properties, Adaugo Mary-Frances Enuka

Theses and Dissertations

Electrospinning is a widely used technique for producing nanofibers with diverse applications. This method uses polymer solutions and strong electric fields to generate nano-sized fibers with unique properties. This study presents a novel manufacturing approach that enables the production of electrospun poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) nanofiber yarns using an automated parallel track system and an adjustable roll-to-roll collector. This research examines the impact of an automated post-drawing system on the polymer chain orientation, crystallinity, tensile strength, morphology, and piezoelectric properties of PVDF-HFP nanofiber yarn. The results reveal that the post-drawing process significantly improves molecular alignment, increases tensile strength, and enhances the …

Chemotherapy Drug Release From Hollow Core Polyethyleneimine Nanoparticles, Brian Boyle

Theses and Dissertations

Novel anticancer delivery vehicles are arising from engineered nanostructured materials. This work involved the synthesis, characterization, and release of novel hollow core polymeric nanoparticles (HCPNPs) for controlled drug delivery. Polyethyleneimine (PEI) was bound to AuNPs, forming polymer-shell nanoparticles with dissolution of the gold core via iodine forming HCPNPs with drug intercalated DNA bound to the surface. A high density (85 molecules/particle) of DNA intercalated with daunorubicin was conjugated. Particles were spherical with an average diameter of 105.7 ± 17.3 nm and zeta potential of 20.4 ± 3.54 mV. We hypothesize the DNA backbone electrostatically condensed to the primary amines on …

Python Tooth–Inspired Fixation Device For Enhanced Rotator Cuff Repair, Iden Kurtaliaj, Ethan D. Hoppe, Yuxuan Huang, David Ju, Jacob A. Sandler, Donghwan Yoon, Lester J. Smith, Silvio Torres Betancur, Linda Effiong, Thomas Gardner, Liana Tedesco, Sohil Desai, Victor Birman, William N. Levine

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Rotator cuff repair surgeries fail frequently, with 20 to 94% of the 600,000 repairs performed annually in the United States resulting in retearing of the rotator cuff. The most common cause of failure is sutures tearing through tendons at grasping points. To address this issue, we drew inspiration from the specialized teeth of snakes of the Pythonoidea superfamily, which grasp soft tissues without tearing. To apply this nondamaging gripping approach to the surgical repair of tendon, we developed and optimized a python tooth–inspired device as an adjunct to current rotator cuff suture repair and found that it nearly doubled repair …

Tuning Polymer Glass Formation Behavior And Understanding Time-Temperature Superposition Breakdown With Oligomeric Additives, Peijing Yue

USF Tampa Graduate Theses and Dissertations

The glass transition of polymer melts has been investigated for over a century. Even so, there are still many unanswered questions about the underlying physics. Countless theories were proposed to explain and define glass transition temperatures. More specifically, both the origins of the glass transition and underlying classification of glass as a state of matter remain unresolved. Time-temperature superposition (TTS) is one of the most widely used techniques to characterize the viscoelastic behavior of a material and the mechanical behavior and is employed to reduce the measuring time during characterization. Despite the widespread use of TTS, many authors have reported …

Electronic Properties Of Group-Iii Nitride Semiconductors And Device Structures Probed By Thz Optical Hall, Nerijus Armakavicius, Philipp Kühne, Alexis Papamichail, Hengfang Zhang, Sean Knight, Axel Persson, Vallery Stanishev, Jr-Tai Chen, Plamen Paskov, Mathias Schubert, Vanya Darakchieva

Department of Electrical and Computer Engineering: Faculty Publications

Group-III nitrides have transformed solid-state lighting and are strategically positioned to revolutionize high-power and high-frequency electronics. To drive this development forward, a deep understanding of fundamental material properties, such as charge carrier behavior, is essential and can also unveil new and unforeseen applications. This underscores the necessity for novel characterization tools to study group-III nitride materials and devices. The optical Hall effect (OHE) emerges as a contactless method for exploring the transport and electronic properties of semiconductor materials, simultaneously offering insights into their dielectric function. This nondestructive technique employs spectroscopic ellipsometry at long wavelengths in the presence of a magnetic …

Automated Flood Prediction Along Railway Tracks Using Remotely Sensed Data And Traditional Flood Models, Abdul Rashid Zakaria, Thomas Oommen, Pasi Lautala

Michigan Tech Publications

Ground hazards are a significant problem in the global economy, costing millions of dollars in damage each year. Railroad tracks are vulnerable to ground hazards like flooding since they traverse multiple terrains with complex environmental factors and diverse human developments. Traditionally, flood-hazard assessments are generated using models like the Hydrological Engineering Center–River Analysis System (HEC-RAS). However, these maps are typically created for design flood events (10, 50, 100, 500 years) and are not available for any specific storm event, as they are not designed for individual flood predictions. Remotely sensed methods, on the other hand, offer precise flood extents only …

Synthesis And Characterization Of Vanadium Nitride/Carbon Nanocomposites, Helia Magali Morales, Horacio Vieyra, David A. Sanchez, Elizabeth M. Fletes, Michael Odlyzko, Timothy P. Lodge, Victoria Padilla-Gainza, Mataz Alcoutlabi, Jason Parsons

Mechanical Engineering Faculty Publications

The present work focuses on the synthesis of a vanadium nitride (VN)/carbon nanocomposite material via the thermal decomposition of vanadyl phthalocyanine (VOPC). The morphology and chemical structure of the synthesized compounds were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), Fourier transformed infrared spectroscopy (FTIR), X-ray diffraction (XRD), and X-ray photoemission spectroscopy (XPS). The successful syntheses of the VOPC and non-metalated phthalocyanine (H2PC) precursors were confirmed using FTIR and XRD. The VN particles present a needle-like morphology in the VN synthesized by the sol-gel method. The morphology of the VN/C composite material exhibited small …