Digital Commons Network^™

Construction Of A Derivative Of Agrobacterium Tumefaciens C58 That Does Not Mutate To Tetracycline Resistance, Zhao-Qing Luo, Thomas E. Clemente, Stephen K. Farrand

Nebraska Center for Biotechnology: Faculty and Staff Publications

Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tet_C58, that is present in the genome of this strain. By deleting the tet_C58 locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants.

Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh

Faculty Works

Hybridization of complementary oligonucleotides mediated by a cationic surfactant at the water/hexane interface leads to hydrophobic, double-helical DNA which may be readily phase transferred to the organic phase and cast into thin films on solid substrates.

Sharing Health-Care Records Over The Internet, Jane Grimson, Gaye Stephens, Benjamin Jung, William Grimson, Damon Berry, Sebastien Pardon

Articles

Presents a novel approach to sharing electronic health-care records that leverages the Internet and the World Wide Web, developed as part of two European Commission-funded projects, Synapses and SynEx. The approach provides an integrated view of patient data from heterogeneous, distributed information systems and presents it to users electronically. Synapses and SynEx illustrate a generic approach in applying Internet technologies for viewing shared records, integrated with existing health computing environments. Prototypes have been validated in a variety of clinical domains and health-care settings

Advantages Of Using Fuzzy Class Memberships In Self-Organizing Map And Support Vector Machines, Sunghwan Sohn, Cihan H. Dagli

Engineering Management and Systems Engineering Faculty Research & Creative Works

The self-organizing map (SOM) is naturally unsupervised learning, but if a class label is known, it can be used as the classifier. In a SOM classifier, each neuron is assigned a class label based on the maximum class frequency and classified by a nearest neighbor strategy. The drawback when using this strategy is that each pattern is treated by equal importance in counting class frequency regardless of its typicalness. For this reason, the fuzzy class membership can be used instead of crisp class frequency and this fuzzy membership-label neuron provides another perspective of a feature map. This fuzzy class membership …

Webquests As An Integrative Experience In Introductory Environmental Engineering, Mary P. Cardenas

All HMC Faculty Publications and Research

An Integrative Experience was developed as part of an introductory environmental engineering course at Harvey Mudd College. The Integrative Experience was developed to help the college students consider the relationship of science and technology with contemporary society. Junior and senior students enrolled in the course designed WebQuests for middle school students. The WebQuests involved the integration of an environmental issue, problem, or technology and societal effects. WebQuests were designed for a middle school audience in order to expand the amount of engineering outreach that is done at the K-12 level. The HMC students chose WebQuests topics including nuclear and alternative …

Purification And Characterization Of Crystallins By Aqueous Two-Phase Extraction, Ondrea Bermudez, Daniel Forciniti

Chemical and Biochemical Engineering Faculty Research & Creative Works

Crystallins are a family of water-soluble proteins that constitute up to 90% of the water-soluble proteins in mammalian eye lenses. We present in this paper an alternative purification method for these proteins using polyethylene glycol/dextran aqueous two-phase extraction. Under the appropriate conditions, we were able to recover the γ-crystallin fraction essentially free of the remaining proteins. High concentrations of salt at a neutral pH maximize the recovery of γ-crystallins in the top phase and minimize the contamination by the other proteins present in the lenses. The proposed protocol decreases the separation time by about 50%. The complex partition behavior observed …

Self-Consistent Simulations Of Electroporation Dynamics In Biological Cells Subjected To Ultrashort Electrical Pulses, R. P. Joshi, Q. Hu, R. Aly, K. H. Schoenbach, H. P. Hjalmarson

Bioelectrics Publications

The temporal dynamics of electroporation of cells subjected to ultrashort voltage pulses are studied based on a coupled scheme involving the Laplace, Nernst-Plank, and Smoluchowski equations. A pore radius dependent energy barrier for ionic transport, accounts for cellular variations. It is shown that a finite time delay exists in pore formation, and leads to a transient overshoot of the transmembrane potential V_mem beyond 1.0 V. Pore resealing is shown to consist of an initial fast process, a 10⁻⁴s delay, followed by a much slower closing at a time constant of about 10 ⁻¹s. This establishes a …

Kentucky Water Resources Research Institute Annual Technical Report Fy 2000, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The FY 2000 Annual Technical Report for Kentucky consolidates the reporting requirements of the Section 104(b) base grant and previous regional competitive grant awards in a single technical report that includes: 1) a synopsis of each ongoing research project and each project completed during the period, 2) a list of related reports published, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, and 5) notable achievements and awards during the year.

Comparison Of Two B-Polynomial Methods: Application To The Identification Of Time Delayed Processes, Ruiyao Gao, Aidan O'Dwyer

Conference papers

An extensive literature exists on the estimation of the model parameters of time delayed processes. The objective of this paper is to review the main B-polynomial approaches to the delay estimation problem. More specifically, two algorithms are discussed and compared theoretically and in simulation. The algorithms involve various procedures to assess how the model relates to the observed data, to a priori knowledge, and to the algorithms’ intended use. Simulation results indicate that both the algorithms can suitably identify the model parameters, including the time delay. This paper also discusses advantages and disadvantages of the methods and gives some guidelines …

Development Of A Low-Cost, Self-Calibrating Stream Gaging Station, Gilberto Urroz, Robert C. Leines, Garryn L. Perret, Jared M. Holland, Brian E. Hunsaker

Reports

Executive Summary: The present report presents a review of the state-of-the-art technologies for the development of a low-cost, self-calibrating stream gaging station. The research was conducted by the Utah Water Research Laboratory with funding provided by the Utah Division of Water Resources through Project RP 110080, March 1998. The report presents a review of the current practices in stream gaging and how technologies such as remote data telemetry through the use of satellite, cellular phone communications, and radio telemetry can be used for the real-time transmission of data. The study also includes the use of Global Positioning System (GPS) for …

Nebraska Summary 389: Fendt 920 Vario Diesel, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA

The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data.

EXPLANATION OF THE TEST PROCEDURES

Purpose

The purpose of …

Nebraska Summary 390: Fendt 924 Vario Diesel, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA

The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data.

EXPLANATION OF THE TEST PROCEDURES

Purpose

The purpose of …

Nebraska Summary 388: Fendt 918 Vario Diesel, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA

The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data.

EXPLANATION OF THE TEST PROCEDURES

Purpose

The purpose of …

Tractor Test 1780a: White 8785, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Tractor Test 1781: White 8510, Agco Dt160, Agco Allis 9755, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Tractor Test 1795: White 8810, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Design And Implementation Of A Biologically Realistic Olfactory Cortex In Analog Vlsi, Jose C. Principe, Vitor G. Tavares, John G. Harris, Walter J. Freeman

Electrical and Computer Engineering Faculty Research & Creative Works

This paper reviews the problem of translating signals into symbols preserving maximally the information contained in the signal time structure. In this context, we motivate the use of nonconvergent dynamics for the signal to symbol translator. We then describe a biologically realistic model of the olfactory system proposed by Walter Freeman that has locally stable dynamics but is globally chaotic. We show how we can discretize Freemans model using digital signal processing techniques, providing an alternative to the more conventional Runge-Kutta integration. This analysis leads to a direct mixed signal {analog amplitude/discrete time) implementation of the dynamical building block that …

Computer Viruses, S. R. Subramanya, Natraj Lakshminarasmhan

Electrical and Computer Engineering Faculty Research & Creative Works

No abstract provided.

Volume Management In San Environment, Chang-Soo Kim, Gyoung-Bae Kim, Bum-Joo Shin

Electrical and Computer Engineering Faculty Research & Creative Works

Logical volume managers have long been key components of a storage system. Their key features are creation of logical or virtual views of physical storage devices and support for various software RAID levels. These make it possible to overcome the limits to capacity, availability and performance of a physical storage device. Most logical volume managers are operated in a single system environment. They are not adequate for SAN (storage area network) environments where several hosts share and access a logical volume at the same time. Some recent logical volume managers are run in a multi-host environment. However, they cannot support …

A Dynamic Programming-Based Approach To Microarray Image Comparison And Registration, Sunil Bosco Rodrigues

Masters Theses

"Microarray technology is increasingly used as a means of high throughput analysis of human, non-human and plant genomes. Manual methods of array production have inherent imperfections and variations of the quality of output data derived from these arrays. A dynamic programming-based approach is presented to validate the quality of such data prior to insertion into evolving databases in order to assign reliably, genetic aberrations to bio-medical functions. Dynamic programming is used for comparing normal and tumor microarray image pairs and a cost function is developed which gives a value representing the difference between the images. Quality of the image pairs …

Focal Osteolysis At The Junctions Of A Modular Stainless-Steel Femoral Intramedullary Nail, Darron M. Jones, Lawrence Marsh, James V. Nepola, Joshua J. Jacobs, Anastasia K. Skipor, Robert M. Urban, Jeremy L. Gilbert, Josheph A. Buckwater

Biomedical and Chemical Engineering - All Scholarship

Background:

During routine follow-up of patients treated with a three-piece stainless-steel modular femoral nail, osteolysis and periosteal reaction around the modular junctions of some of the nails were noted on radiographs. The purpose of this study was to evaluate the prevalence, etiology, and clinical relevance of these radiographic findings.

Methods:

Forty-four femoral fractures or nonunions in forty-two patients were treated with a modular stainless-steel femoral intramedullary nail. Seventeen nails were excluded, leaving twenty-seven intramedullary nails in twenty-seven patients for this study. All patients had had a femoral diaphyseal fracture; nineteen had had an acute fracture and eight, a nonunion. These …

Experiments On Adaptive Techniques For Host-Based Intrusion Detection, Timothy Draelos, Michael Collins, David Duggan, Edward Thomas, Donald C. Wunsch

Electrical and Computer Engineering Faculty Research & Creative Works

This research explores four experiments of adaptive host-based intrusion detection (ID) techniques in an attempt to develop systems that can detect novel exploits. The technique considered to have the most potential is adaptive critic designs (ACDs) because of their utilization of reinforcement learning, which allows learning exploits that are difficult to pinpoint in sensor data. Preliminary results of ID using an ACD, an Elman recurrent neural network, and a statistical anomaly detection technique demonstrate an ability to learn to distinguish between clean and exploit data. We used the Solaris Basic Security Module (BSM) as a data source and performed considerable …

Spatial Patterns And Floodplain Contributions Of Mining-Related Contaminants In Chat Creek Watershed, Southwest Missouri, Jimmy Trimble

Graduate Theses/Dissertations

Past mining activities have left a legacy of water quality problems in the Tri-State Mining District. Contemporary environmental risks stem from the release of lead and zinc from mine tailings previously dispersed far downstream in alluvial deposits as well as abandoned tailings piles. This study examines mining-related heavy metal contamination in the Chat Creek Watershed. Chat Creek drains the area near Aurora, Missouri on the eastern edge of the Tri-State District where mining occurred from 1886 to about 1930. This study identifies the spatial distribution of metal contamination in fluvial sediments and quantifies the role of bank erosion as a …

Test 1794: White 8710 Also Agco Allis 9775 (Cummins Engine) Also Agco Dt200, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Test 1792: Massey Ferguson 2210 Diesel 15-Speed, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA

The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data.

EXPLANATION OF THE TEST PROCEDURES

Purpose

The purpose of …

Test 1793: Massey Ferguson 2220 Diesel 12-Speed, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA

The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data.

EXPLANATION OF THE TEST PROCEDURES

Purpose

The purpose of …

Nebraska Summary 391: Fendt 926 Vario Diesel, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA

The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data.

EXPLANATION OF THE TEST PROCEDURES

Purpose

The purpose of …

Entropy Generation Method To Quantify Thermal Comfort, S. C. Boregowda, S. N. Tiwari, S. K. Chaturvedi

Mechanical & Aerospace Engineering Faculty Publications

The present paper presents a thermodynamic approach to assess the quality of human-thermal environment interaction and quantify thermal comfort. The approach involves development of entropy generation term by applying second law of thermodynamics to the combined human-environment system. The entropy generation term combines both human thermal physiological responses and thermal environmental variables to provide an objective measure of thermal comfort. The original concepts and definitions form the basis for establishing the mathematical relationship between thermal comfort and entropy generation term. As a result of logic and deterministic approach, an Objective Thermal Comfort Index (OTCI) is defined and established as a …

Municipal Waste Characteristics And Management In Pantai Dalam, Kuala Lumpur, Ratanam Andythaven

Student Works (2000-2009)

The primary purpose of this research was to study comprehensively the municipal waste generation (rate, quantity), waste characterization including (physical, chemical and biological parameters), heavy metal content, waste recovery and recycling, and socio economic aspects of municipal solid waste (MSW) generation and management in Taman Bukit Angkasa, Pantai Dalam, Kuala Lumpur and the surrounding areas. The waste characterization and management was determined with actual field observations coupled with questionnaire survey from a random sample of 250 households (200 flats houses, 25 long houses and 25 squatter residences). The quantification and other parameters (heavy metals, water extractable nutrients, nitrogen, pH, acidity, …

Municipal Wastewater Treatment Using Sequencing Batch Reactor (Sbr) Under Tropical Conditions, Maria Violetta Thomas

Student Works (2000-2009)

In this research project a Sequencing Batch Reactor (SBR) plant was tested for performance in terms of Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and Suspended Solids (SS), under different temporal conditions. In addition, the removal of biological nutrients, namely ammoniacal nitrogen, nitrate nitrogen and phosphorous in the form of phosphate, from the sewage, with anaerobic and aerobic operations using SBR was studied. The performance of the SBR plant was observed under two operating conditions whereby the variable studied was the total cycle time of the SBR process; i.e. 8-hour cycle mode and 6-hour cycle mode. The effluent quality …