Digital Commons Network^™

Characterization Of An Electron Conduit Between Bacteria And The Extracellular Environment, Robert Hartshorne, Catherine Reardon, Daniel Ross, Jochen Nuester, Thomas Clarke, Andrew Gates, Paul Mills, James K. Fredrickson, John M. Zachara, Liang Shi, Alexander Beliaev, Matthew Marshall, Ming Tien, Susan Brantley, Julea N. Butt, David J. Richardson

United States Department of Energy: Publications

A number of species of Gram-negative bacteria can use insoluble minerals of Fe(III) and Mn(IV) as extracellular respiratory electron acceptors. In some species of Shewanella, deca-heme electron transfer proteins lie at the extracellular face of the outer membrane (OM), where they can interact with insoluble substrates. To reduce extracellular substrates, these redox proteins must be charged by the inner membrane/periplasmic electron transfer system. Here, we present a spectro-potentiometric characterization of a trans-OM icosa-heme complex, MtrCAB, and demonstrate its capacity to move electrons across a lipid bilayer after incorporation into proteoliposomes. We also show that a stable MtrAB …

Artificial Insemination In South American Camelids And Wild Equids, G. P. Adams, M. H. Ratto, C. W. Collins, D. R. Bergfelt

United States Environmental Protection Agency: Publications

An overview of the present status of the use of artificial insemination (AI) in South American camelids and wild equids is offered. Technical aspects of semen collection, dilution and cryopreservation have limited the development and use of AI in camelid and equid species. To-date, efficiency is low but progress has been made and viable offspring have been produced through the use of AI in domestic South American camelids using both fresh and frozen semen. The origin, composition, and function of the viscous component of camelid seminal plasma remain a mystery and an obvious area for future research. A better understanding …

Foodweb Modeling For Polychlorinated Biphenyls (Pcbs) In The Twelvemile Creek Arm Of Lake Hartwell, South Carolina, Usa, Brenda Rashleigh, M. Craig Barber, David M. Walters

United States Environmental Protection Agency: Publications

PCBs from the Sangamo-Weston Superfund Site near Clemson, South Carolina, USA, were released into the Twelvemile Creek Arm of Lake Hartwell until the early 1990s. Monitoring data have shown that while PCB concentration in sediments declined since 1995, PCB concentrations in fish have remained elevated, e.g., largemouth bass (Micropterus salmoides) concentrations have ranged from 5 to 10 ppm. The EPA aquatic ecosystem model AQUATOX was applied to this system to better characterize foodweb dynamics that lead to biomagnification of PCBs. The model was calibrated with observed fish biomass data. Simulated PCB loading over a 12-year period provided a …

Comparing The Mutagenicity Of Toxaphene After Aging In Anoxic Soils And Accumulating In Fish, James C. Young, Anne D. Freeman, Robert M. Bruce, Douglas Williams, Keith Maruya

United States Environmental Protection Agency: Publications

A test program was conducted to evaluate the mutagenicity of toxaphene residuals extracted from aged soils and from fish collected in creeks near a toxaphene-contaminated site. The ultimate objective was to determine if the residual toxaphene congeners were more or less mutagenic than those in technical- grade toxaphene.The study showed that the mutagenicity of the bioaccumulated toxaphenecongeners in fish, expressed as colony revertants per mg of residual toxaphene, was no greater than that of technical-grade toxaphene.Themutagenicimpactofthetoxapheneresidualsinagedsoilstatistically was less than that for technical-grade toxaphene. Two specific congeners, a hexachlorobornane(labeled Hx-Sd) and a heptachlorobornane (labeled Hp-Sd), were found to accumulate over time …

Chipless Rfid Sensor Tag System With Microstrip Transmissionline Based Id Generation Schemes, Sudhir Shrestha

Doctoral Dissertations

This dissertation presents a chipless radio frequency identification (RFID) sensor tag system consisting of passive chipless RFID sensor tags and specialized reader. The chipless sensor tags are fabricated on a flexible substrate and contain an ID generation circuit, a sensor, and a microstrip antenna. The ID generation circuit consists of meandered microstrip transmission lines and uses a novel reflection and delay based ID generation scheme. The scheme, using an input RF pulse, constructs an on-off keying (OOK) or pulse position modulated (PPM) signal pattern representing a unique ID code. Two transmission lines and OOK representation are used and the generation …

Simulation Of Corn Stover And Distillers Grains Gasification With Aspen Plus, Ajay Kumar, Hossein Noureddini, Yaşar Demirel, David Jones, Milford Hanna

Yaşar Demirel Publications

A model was developed to simulate the performance of a lab‐scale gasifier and predict the flowrate and composition of product from given biomass composition and gasifier operating conditions using Aspen Plus software. Mass balance, energy balance, and minimization of Gibbs free energy during the gasification were applied to determine the product gas composition. Carbon conversion efficiency and tar content were provided to the model as inputs as these could not be predicted by the model based on minimization of Gibbs free energy. Experiments for validation of the model were performed on a lab‐scale fluidized bed gasifier using corn stover and …

The Ethical Phenomenon Of Gm-Corn: Anger, Anxiety, And Arrogance In Crossing American Borders, Jules Simon

Departmental Papers (Philosophy)

In terms of phenomenology, I often wonder about the relevance of what I do as a philosopher for the life of those with whom I come into contact. This ‘coming into contact’ happens for me on several levels: as one human among many, as a husband and father and son and brother, as a teacher, as a neighbor, and as country or city dweller. I remember with fondness those times in the late sultry summer months when, as a youth, I would drive with my father to this or that local farm-stand on some remote back road in the hills …

Infrared Light Activated Floating Micro Stimulators For Neuro-Prosthetic Applications, Kimberlyn M. Gray

Doctoral Dissertations

The most common failures in neural stimulation implants are due to interconnect complications such as tissue response, lead migration, and lead breakages. The challenge in eliminating interconnects lies in minimizing device size to maintain spatial selectivity required in the CNS. One approach to this problem is a current generating device that can be stimulated by an external signal, such as light or sound. Here, we report the design, construction and testing of rnicrophotodiode devices that can be stimulated remotely with near-infrared (NIR) light to generate current that can be injected locally into the peripheral nervous system. The use of near-infrared …

Doe Climate Change Research Program: Strategic Plan

United States Department of Energy: Publications

Advance the forefront of climate change research to provide the nation with the scientific knowledge it needs about the effects of greenhouse gas emissions on Earth's climate and biosphere to support effective energy and environmental decision making.

Mineralogical Transformations Controlling Acid Mine Drainage Chemistry, T. Peretyazhko, John M. Zachara, J.-F. Boily, Y. Xia, P.L. Gassman, B.W. Arey, W.D. Burgos

United States Department of Energy: Publications

The role of Fe(III) minerals in controlling acid mine drainage (AMD) chemistry was studied using samples from two AMD sites [Gum Boot (GB) and Fridays-2 (FR)] located in northern Pennsylvania. Chemical extractions, X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) were used to identify and characterize Fe(III) phases. The mineralogical analysis revealed schwertmannite and goethite as the principal Fe(III) phases in the sediments. Schwertmannite particles occurred as sub-micron sized spheroids. Their transformation into goethite occurred at the GB site where poorly-crystallized goethite rich in surface-bound sulfate was initially formed. In contrast, no schwertmannite transformation occurred …

Production And Characterization Of Foam Glass From Container Glass Waste, Dina Mohamed Abdel Alim

Archived Theses and Dissertations

No abstract provided.

Design Of Sea Water Heat Exchanger For Miniature Vapor Compression Cycle, James Hughes

Electronic Theses and Dissertations

Recent advances in the development of miniature vapor compression cycle components have created unique opportunities for heating and cooling applications, specifically to human physiological requirements that arise in extreme environments. Diving in very cold water between 1.7 and 5°C requires active heating because passive thermal insulation has proven inadequate for long durations. To maintain diver mobility and cognitive performance, it is desirable to provide 250 to 300 W of heat from an untethered power source. The use of a miniature vapor compression cycle reduces the amount of power (batteries or fuel cell) that the diver must carry by 2.5 times …

Droplet Flows In Microchannels Using Lattice Boltzmann Method, Amit Gupta

Electronic Theses and Dissertations

Microelectromechanical systems (MEMS) have widespread applications in medical, electronical and mechanical devices. These devices are characterized by the smallest dimension which is at least one micrometer and utmost one millimeter. Rapid progress in the manufacture and utilization of these microdevices has been achieved in the last decade. Current manufacturing techniques of such devices and channels include surface silicon micromachining; bulk silicon micromachining; lithography; electrodeposition and plastic molding; and electrodischarge machining (EDM). In recent years, electrostatic, magnetic, electromagnetic and thermal actuators, valves, gears and diaphragms of dimensions of hundred microns or less have been fabricated successfully. Sensors have been manufactured that …

Learning Semantic Features For Visual Recognition, Jingen Liu

Electronic Theses and Dissertations

Visual recognition (e.g., object, scene and action recognition) is an active area of research in computer vision due to its increasing number of real-world applications such as video (image) indexing and search, intelligent surveillance, human-machine interaction, robot navigation, etc. Effective modeling of the objects, scenes and actions is critical for visual recognition. Recently, bag of visual words (BoVW) representation, in which the image patches or video cuboids are quantized into visual words (i.e., mid-level features) based on their appearance similarity using clustering, has been widely and successfully explored. The advantages of this representation are: no explicit detection of objects or …

New Computational Approaches For Multiple Rna Alignment And Rna Search, Daniel Deblasio

Electronic Theses and Dissertations

In this thesis we explore the the theory and history behind RNA alignment. Normal sequence alignments as studied by computer scientists can be completed in O(n2) time in the naive case. The process involves taking two input sequences and finding the list of edits that can transform one sequence into the other. This process is applied to biology in many forms, such as the creation of multiple alignments and the search of genomic sequences. When you take into account the RNA sequence structure the problem becomes even harder. Multiple RNA structure alignment is particularly challenging because covarying mutations make sequence …

Alliances In Graphs: Parameterized Algorithms And On Partitioning Series-Parallel Graphs, Rosa Enciso

Electronic Theses and Dissertations

Alliances are used to denote agreements between members of a group with similar interests. Alliances can occur between nations, biological sequences, business cartels, and other entities. The notion of alliances in graphs was first introduced by Kristiansen, Hedetniemi, and Hedetniemi in . A defensive alliance in a graph G = (V, E) is a non empty set S ⊆ V where, for all x ∈ S, |N[x] ∩ S| ≥ |N[x] − S|. Consequently, every vertex that is a member of a defensive alliance has at least as many vertices defending it as there are vertices attacking it. Alliances can …

Delay Modeling And Long-Range Predictive Control Of Czochralski Growth Process, Dhaval Shah

Electronic Theses and Dissertations

This work presents the Czochralski growth dynamics as time-varying delay based model, applied to the growth of La3Ga5.5Ta0.5O14 (LGT) piezoelectric crystals. The growth of high-quality large-diameter oxides by Czochralski technique requires the theoretical understanding and optimization of all relevant process parameters, growth conditions, and melts chemistry. Presently, proportional-integral- derivative (PID) type controllers are widely accepted for constant-diameter crystal growth by Czochralski. Such control systems, however, do not account for aspects such as the transportation delay of the heat from crucible wall to the crystal solidification front, heat radiated from the crucible wall above the melt surface, and varying melt level. …

Flow Visualization In Microfluidic Expansion And Mixing, Ehsan Yakhshi-Tafti

Electronic Theses and Dissertations

Micro particle image velocimetry (microPIV) is a non-intrusive tool for visualizing flow in micron-scale conduits. Using this investigative instrument, two experimental studies were performed to understand flow behaviors in microfluidic channels - a sudden expansion step flow and laminar velocity profile variation in diffusion driven mixing. First, flow in a backward facing step feature (1:5 expansion ratio) in a microchannel was taken as the subject of microPIV flow visualization. The onset and development of a recirculation flow was studied as a function of flow rate. This flow pattern was further used to investigate two major parameters affecting microPIV measurements; the …

The Integrated User Experience Evaluation Model: A Systematic Approach To Integrating User Experience Data Sources, Roberto Champney

Electronic Theses and Dissertations

Evaluating the user experience (UX) associated with product interaction is a challenge for current human-systems developers. This is largely due to a lack of theoretical guidance for directing how best to assess the UX and a paucity of tools to support such evaluation. This dissertation provided a framework and tools for guiding and supporting evaluation of the user experience. This doctoral research involved reviewing the literature on UX, using this knowledge to build first build a theoretical model of the UX construct and later develop a theoretical model to for the evaluation of UX in order to aid evaluators – …

Managing Livestock Manure To Protect Environmental Quality, Brian L. Benham, Richard B. Ferguson, Christopher G. Henry, Michael A. Reynolds, Charles A. Shapiro, James P. Stack, Charles S. Wortmann

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Proper utilization of livestock manure is a major environmental concern. Livestock manure has typically been considered a ‘waste product’ of livestock production. A more accurate name for this material is a ‘recycled resource’ from the production of livestock. By managing livestock manure, the producer has a second chance to utilize the nutrients that have already been paid for but not fully utilized by the animal. Collecting, containing, and properly spreading livestock manure will incur additional costs for the producer. However, these costs can be offset or justified by the producer in terms of the yield enhancement and nutrient credits that …

Integrated Capture And Spectroscopic Detection Of Viruses, Crystal A. Vargas, Allison A. Wilhelm, Jeremy Williams, Pierre Lucas, Kelly A. Reynolds, Mark R. Riley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The goal of this work is to develop an online monitoring scheme for detection of viruses in flowing drinking water. The approach applies an electrodeposition process that is similar to the use of charged membrane filters previously employed for collection of viruses from aqueous samples. In the present approach, charged materials are driven onto a robust optical sensing element which has high transparency to infrared light. A spectroscopic measurement is performed using the evanescent wave that penetrates no more than 1 m from the surface of an infrared optical element in an attenuated total reflectance measurement scheme. The infrared measurement …

Investigation Of River Eutrophication As Part Of A Low Dissolved Oxygen Tmdl Implementation, Gary M. Litton, W. T. Stringfellow, J. Herr, M. Brunell, S. Borglin

All Faculty Articles - School of Engineering and Computer Science

In the United States, environmentally impaired rivers are subject to regulation under total maximum daily load (TMDL) regulations that specify watershed wide water quality standards. In California, the setting of TMDL standards is accompanied by the development of scientific and management plans directed at achieving specific water quality objectives. The San Joaquin River (SJR) in the Central Valley of California now has a TMDL for dissolved oxygen (DO). Low DO conditions in the SJR are caused in part by excessive phytoplankton growth (eutrophication) in the shallow, upstream portion of the river that create oxygen demand in the deeper estuary. This …

Identification Of Multiple Oscillation States Of Carbon Nanotube Tipped Cantilevers Interacting With Surfaces In Dynamic Atomic Force Microscopy, Mark Strus, Arvind Raman

Birck and NCN Publications

Carbon nanotubes (CNTs) have gained increased interest in dynamic atomic force microscopy (dAFM) as sharp, flexible, conducting, nonreactive tips for high-resolution imaging, oxidation lithography, and electrostatic force microscopy. By means of theory and experiments we lay out a map of several distinct tapping mode AFM oscillation states for CNT tipped AFM cantilevers: namely, noncontact attractive regime oscillation, intermittent contact with CNT slipping or pinning, or permanent contact with the CNT in point or line contact with the surface while the cantilever oscillates with large amplitude. Each state represents fundamentally different origins of CNT-surface interactions, CNT tip-substrate dissipation, and phase contrast …

Parallel Reactive Molecular Dynamics: Numerical Methods And Algorithmic Techniques, Hasan Metin Aktulga, Joseph C. Fogarty, Sagar A. Pandit, Ananth Y. Grama

PRISM: NNSA Center for Prediction of Reliability, Integrity and Survivability of Microsystems

No abstract provided.

The Plant Ionome Coming Into Focus, Lorraine Williams, David Salt

Cyber Center Publications

92 elements have been identified on earth and 17 of these are known to be essential to all plants. The essential elements required in relatively large amounts (>0.1% of dry mass) are called macronutrients and include C, H, O, N, S, P, Ca, K, Mg. Those required in much smaller amounts (<0.01% of dry mass) are referred to as micronutrients or trace elements and include Ni, Mo, Cu, Zn, Mn, B, Fe, and Cl. Plant growth and development depends on a balanced supply of these essential elements and thus the plant has a range of homeostatic mechanisms operating to ensure that this is maintained. Beneficial elements which promote growth and may be essential to some taxa, include Na, Co, Al, Se and Si. Elements such as the heavy metal Cd and the metalloid As have no demonstrated biological function in plants, but are nevertheless taken up and cause severe toxicity in most plant species. The concept for this special issue is the plant ionome, a word coined to encompass all these elements and allow focussed discussion and investigations on the mechanisms that co-ordinately regulate these elements in response to genetic and environmental factors reviewed in Salt et al., 2008).

Gold Nanorods As Contrast Agents For Biological Imaging: Optical Properties, Surface Conjugation And Photothermal Effects, Ling Tong, Qingshan Wei, Alexander Wei, Ji-Xin Cheng

Other Nanotechnology Publications

Gold nanorods (NRs) have plasmon-resonant absorption and scattering in the near-infrared (NIR) region, making them attractive probes for in vitro and in vivo imaging. In the cellular environment, NRs can provide scattering contrast for darkfield microscopy, or emit a strong two-photon luminescence due to plasmon-enhanced two-photon absorption. NRs have also been employed in biomedical imaging modalities such as optical coherence tomography or photoacoustic tomography. Careful control over surface chemistry enhances the capacity of NRs as biological imaging agents by enabling cell-specific targeting, and by increasing their dispersion stability and circulation lifetimes. NRs can also efficiently convert optical energy into heat, …

Taproot™ Technology: Tree Coring For Fast, Noninvasive Plume Delineations, Joel Gerard Burken, Sallie Bailey, Matt Shurtliff, Jeff Mcdermott

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

In Efforts to Evaluate the Use of Plants as a Forensic Tool for Delineating Contaminated Soil and Groundwater, a Laboratory Experiment and a Field Sampling Effort Were Undertaken. Site Assessments Are Often Costly and Inaccurate, Requiring Multiple Mobilizations to Hone in on Source Areas and Getting Accurate Estimates of Contaminant Extent and Distribution. as These Extensive Site Delineations Take Place, Valuable Time and Resources Are Lost. the Findings of This Study Show that Plants Can Be Used as a Tool to Evaluate a Variety of Subsurface Contaminants, Either in the Vadose Zone or in the Saturated Zone. in the First …

Magnitude And Variability In Emissions Savings In The Corn-Ethanol Life Cycle From Feeding Co-Products To Livestock, Virgil R. Bremer, Adam Liska, Terry J. Klopfenstein, Galen E. Erickson, Haishun Yang, Daniel T. Walters, Kenneth G. Cassman

Adam Liska Papers

Conclusions

• Co-product GHG emissions credit varied by >2-fold, from 11.5 to 28.3 gCO₂e per MJ of ethanol produced

• Co-product GHG emissions credit depend on
-types of co-products produced
-proportion fed to beef cattle vs. diary or swine
-location of corn production; the CP credit is highest in regions where GHG kg^-1grain are highest

• Depending on CP production types and feeding livestock classes, corn-ethanol net life cycle GHG intensity is 44-56 gCO₂e per MJ

• Midwest corn-ethanol reduces GHG emissions compared to gasoline by 47% on average, with co-products offsetting 23% of …

Differential Regulation Of Vitamin D Receptor (Vdr) By P53, P63 And P73, Ramakrishna Kommagani

Browse all Theses and Dissertations

The tumor suppressor p53 is the single most altered gene in human cancers. p53 homologues, p63 and p73 play a major role in development and in human cancer. Both p63and p73-null mice exhibit profound developmental abnormalities, suggesting a vital role for p63 and p73 in development. Although the role of p73 in human cancers is well established, the role of p63 still remains to be understood. While p63 plays a major role in development, p73 plays a major role in tumor suppression as well as in development. Although a functional co-operation is evident between each member of p53 family, additional …

Determining The Effect Of Speaker's Gender And Speech Synthesis On Callsign Acquisition Test (Cat) Results, Jasminkumar B. Soni

Browse all Theses and Dissertations

Effective and efficient speech communication is one of the leading factors for success of battlefield operation. With the increases in the levels of gender diversity in military services, it is important to assess the effectiveness of voice for both genders in communication systems. The purpose of this research study was to determine the effect of the speaker's voice (male and female) on the speech intelligibility (SI) performance of the Callsign Acquisition Test (CAT). In addition, the effects of synthesized speech were evaluated. The CAT test is a new SI test that has been developed for military use. A group of …