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Ultrasound-Induced Phase Change Of Emulsion Droplets For Targeted Gene And Drug Delivery, James R. Lattin

Theses and Dissertations

This dissertation explores the potential of using perfluorocarbon emulsion droplets to add an ultrasound-sensitive element to drug delivery systems. These emulsion droplets may be induced to vaporize with ultrasound; during the rarefactional phase of an ultrasound wave, the pressure around the droplets may fall below the vapor pressure of the liquid forming the emulsion, providing a thermodynamic potential for vaporization. This ultrasound-induced phase change of the emulsion droplet could release therapeutics attached to the droplet surface or aid in drug delivery due to mechanical effects associated with vaporization and expansion, similar to the ability of cavitating bubbles to aid in …

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, And Dna Stretching, Kevin D. Dorfman, Scott B. King, Daniel W. Olson, Joel D. P. Thomas, Douglas R. Tree

Faculty Publications

This review addresses methods for obtaining sequence information directly from unamplified genomic length DNA. Our generic starting point is a large piece of DNA that contains many thousands of base pairs (kilobase pairs, kbp) or even millions of base pairs (megabase pairs, Mbp). We would like to determine the genomic distance between two repeats of a given sequence, indicated by the red dots on the coiled DNA molecule in Figure 1. Let us consider the case where these sequences are restriction sites, which can be selectively cut using proteins known as restriction enzymes. For decades, gel electrophoresis served as the …

Quantitative, Spectrally-Resolved Intraoperative Fluorescence Imaging, Pablo A. Valdés, Frederic Leblond, Valerie L. Jacobs, Brian C. Wilson, Keith D. Paulsen, David W. Roberts

Dartmouth Scholarship

Intraoperative visual fluorescence imaging (vFI) has emerged as a promising aid to surgical guidance, but does not fully exploit the potential of the fluorescent agents that are currently available. Here, we introduce a quantitative fluorescence imaging (qFI) approach that converts spectrally-resolved data into images of absolute fluorophore concentration pixel-by-pixel across the surgical field of view (FOV). The resulting estimates are linear, accurate, and precise relative to true values, and spectral decomposition of multiple fluorophores is also achieved. Experiments with protoporphyrin IX in a glioma rodent model demonstrate in vivo quantitative and spectrally-resolved fluorescence imaging of infiltrating tumor margins for the …

Communications And Energy-Harvesting In Nanosensor Networks, Michele C. Weigle

Computer Science Presentations

PDF of a powerpoint presentation from the National Science Foundation (NSF) Workshop on Biological Computations and Communications in Boston, Massachusetts, November 9, 2012. Also available on Slideshare.

Nonlinear Dynamics Of The Atomic Force Microscope At The Liquid-Solid Interface, Daniel Kiracofe, Arvind Raman

Birck and NCN Publications

The measurement of intermolecular forces at the liquid-solid interface is key to many studies of electrochemistry, wetting, catalysis, biochemistry, and mechanobiology. The atomic force microscope (AFM) is unique in its ability to measure and map these forces with nanometer resolution using the oscillating sharp tip of an AFM cantilever. These surface forces are only measured by observing the changes they induce in the dynamics of the resonant AFM probe. However, AFM cantilever dynamics at this interface can be significantly different when compared to air/vacuum environments due to the nature of nanoscale forces at the interface and the low-quality factors in …

Load-Relaxation Properties Of The Human Trunk In Response To Prolonged Flexion: Measuring And Modeling The Effect Of Flexion Angle, Nima Toosizadeh, Maury A. Nussbaum, Babak Bazrgari, Michael L. Madigan

Biomedical Engineering Faculty Publications

Experimental studies suggest that prolonged trunk flexion reduces passive support of the spine. To understand alterations of the synergy between active and passive tissues following such loadings, several studies have assessed the time-dependent behavior of passive tissues including those within spinal motion segments and muscles. Yet, there remain limitations regarding load-relaxation of the lumbar spine in response to flexion exposures and the influence of different flexion angles. Ten healthy participants were exposed for 16 min to each of five magnitudes of lumbar flexion specified relative to individual flexion-relaxation angles (i.e., 30, 40, 60, 80, and 100%), during which lumbar flexion …

The Surface Distribution Of Oil Spills, The Environmental Status Of The Visoka Oilfield, Its Rehabilitation Ways: Case Study, Nevton Kodhelaj, Aida Bode, Piro Zoga, Nevina Koja, Esmeralda Zeqo, Dulian Zeqiraj

UBT International Conference

Visoka oilfield was explored in 1963 through the well G-622. In this oilfield had been drilled more than 170 production wells. Through all this years of its exploitation, mainly because of the outdated technology, the environmental pollution reached really dramatic values. The pollution extends on the Visoka village, affecting so the life for the entire community. The oil spills, the gas emissions and the formation water affected and polluted and ―k illed‖ the Gjanica River, its delta and the Adriatic coast in the Semani beach. Due to the technology and the completely wrong policies, used in the past, the wells …

Water Resources Systems Planning And Management, Piro Zoga, Aida Bode, Ramiz Balla, Nevton Kodhelaj, Spartak Kucaj

UBT International Conference

The most fundamental human needs of water are for drinking, cooking and personal hygiene. In order to fulfill these needs, the quality of the water used should cause no risk to human health. The quality of the water in nature also affects the condition of ecosystems all living organisms depend on. At the same time, humans use water bodies as convenient sinks for the disposal of domestic, industrial and agricultural wastewaters. This, of course, degrades the quality of those water bodies. Water resources management involves the monitoring and management of water quality as much as the monitoring and management of …

Urban Waste Water Discharges And Their Treatment: Case Study Waste Water Treatment Plant Of Kavaja (Wwtpk), Aida Bode, Nevton Kodhelaj, Piro Zoga, Doriana Xhulaj

UBT International Conference

WWTPK is the first plant built sewage purification in the Republic of Albania (RA). This plant it is placed in the Kavaja region, district of Tirana. For this is an important area sewage treatment and waste water treatment according to the standards of the Ministry of Environment, Forestry and Water Management (MoE) in Republic of Albania and not directly discharged untreated in nature. In order to determine productivity of the IPUZ and to predict the future of the operation, the results of the analysis of BOD, COD, SS are produced. According to chemical analyzes carried out in the period from …

Research Of Agronomic And Quality Traits Of Winter Barley Varieties (Hordeum Vulgare L.) Under Growing Conditions In The Republic Of Kosovo, Nexhdet Shala, Bakir Kelmendi

UBT International Conference

The object of this paper is the research of agronomic and quality trains of winter varieties (hordeum vulgare L.) their adaptability and specific reactions towards growing conditions in the Republic of Kosovo. During three year trails on localities in Dukagjini area and area of Kosovo we researched 5 winter barley varieties. Environment has been explored together with estimation of significant parameters of grain yield, hectoliter weight, protein and starch content. Impact of year, location and variety on grain yield, hectoliter grain weight, protein and starch content has been estimated by analysis of variance. Environmental effects of planting barley cultivars in …

Improved Optical Probe For Measuring Phytoplankton Suspension Concentrations Based On Optical Fluoresensce And Absorption, Retno Wigajatri Purnamaningsih, Nining Betawati Prihantini

Makara Journal of Technology

This paper discusses the results of improved optical probe that works according to optical fluorescence and absorption phenomena for measuring the phytoplankton suspension concentrations. Measurements are made on the Scenedesmus sp. culture. The laboratory test has shown that range of concentration from 104 up to around 1 x 106 cells/mL; fluorescence intensity at λ = 685 nm; and logarithmic of transmission intensity at λ = 405 nm are proportional to the cell concentration linear with proportional constant γ = 4 × 10-5 and β = –2 × 10-7 mL/cell respectively.

Vibrations Of Crystal Plates With Surface Structures For Resonator And Sensor Applications, Nan Liu

Department of Engineering Mechanics: Dissertations, Theses, and Student Research

This dissertation is mainly on the theoretical analysis of vibrating crystal plates for acoustic wave resonator and sensor applications. The frequency and mode effects of different surface structures on either or both sides of the crystal plates are the main concerns in this dissertation. These effects are fundamental to the improvement of existing acoustic wave devices, or to the design of new acoustic wave devices, especially new sensors based on these effects.

At first, two-dimensional equations of motion for an anisotropic crystal plate with two thin films on its surfaces are derived by reduction from the three-dimensional equations of anisotropic …

Fiber-Tip Fabry–Perot Interferometric Sensor Based On A Thin Silver Film, Fawen Guo

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

Fiber-optic sensors have many advantages, including small size, light weight, immunity to electromagnetic interference, and the capability of remote sensing. Fiber- tip sensors are fabricated on a fiber tip and are the smallest type of fiber-optic sensor. The fiber-tip sensors have attracted a great deal of attention in past years for pressure, temperature, and acoustic sensing.

In this thesis, a fiber-tip sensor based on an ultra-thin silver film was proposed and demonstrated for highly sensitive and high frequency ultrasonic detection. The film is prepared by the vacuum thermal deposition method and then transferred to the fiber tip. The sensor has …

Inactivation Of Bacterial Opportunistic Skin Pathogens By Nonthermal Dc-Operated Afterglow Atmospheric Plasma, L. C. Heller, C. M. Edelblute, A. M. Mattson, X. Hao, J. F. Kolb

Bioelectrics Publications

AIMS: Multidrug-resistant opportunistic pathogens are clinically significant and require the development of new antimicrobial methods. In this study, Acinetobacter baumannii, Pseudomonas aeruginosa and Staphylococcus aureus cells were exposed to atmospheric plasma on agar plates and in vitro on porcine skin for the purpose of testing bacterial inactivation.

METHODS AND RESULTS: Microbial inactivation at varying exposure durations was tested using a nonthermal plasma jet generated with a DC voltage from ambient air. The observed reduction in colony forming units was quantified as log₁₀ reductions.

CONCLUSIONS: Direct plasma exposure significantly inactivated seeded bacterial cells by approx. 6 log_{10 …}

The Design And Testing Of A Solar Autoclave With Broad Spectrum Sterilization Capabilities, Sarah S. Trabia

Honors College Theses

Energy is difficult to come by in developing countries and this interferes with the ability of doctors to provide good healthcare to their patients. Without decent sterilization, patients can become infected and diseases can spread throughout hospitals. This study involves designing a solar autoclave for developing countries that is as efficient as electrical autoclaves. The design is intended to not require any electrical input and to be affordable to those who cannot afford advanced medical equipment. Once the solar autoclave is designed, it will be tested to determine if it can sterilize equipment against a variety of different bacteria, including …

Noninvasive Optical Characterization Of Muscle Blood Flow, Oxygenation, And Metabolism In Women With Fibromyalgia, Yu Shang, Katelyn Gurley, Brock Symons, Douglas Long, Ratchakrit Srikuea, Leslie J. Crofford, Charlotte A. Peterson, Guoqiang Yu

Biomedical Engineering Faculty Publications

INTRODUCTION: Women with fibromyalgia (FM) have symptoms of increased muscular fatigue and reduced exercise tolerance, which may be associated with alterations in muscle microcirculation and oxygen metabolism. This study used near-infrared diffuse optical spectroscopies to noninvasively evaluate muscle blood flow, blood oxygenation and oxygen metabolism during leg fatiguing exercise and during arm arterial cuff occlusion in post-menopausal women with and without FM.

METHODS: Fourteen women with FM and twenty-three well-matched healthy controls participated in this study. For the fatiguing exercise protocol, the subject was instructed to perform 6 sets of 12 isometric contractions of knee extensor muscles with intensity steadily …

Optimizing The Mechanical Characteristics Of Bamboo To Improve The Flexural Behavior For Biocomposite Structural Application, Jay Lopez

Master's Theses

Global awareness and preservation have spurred increasing interest in utilizing environmentally friendly materials for high-performance structural applications. Biocomposites pose an appealing solution to this issue and are characterized by their sustainable lifecycles, biodegradable qualities, light weight, remarkable strength, and exceptional stiffness. Many of these structural qualities are found in applications that exhibit flexural loading conditions, and this study focuses on improving the bending performance of engineered biocomposite structures. The current application of biocomposites is increasing rapidly, so this expanding research explores other natural constituent materials for biocomposite structures under flexural loading.

The renewable material investigated in this study was experimentally …

Thiol Reactive Probes And Chemosensors, Robert M. Strongin, Lovemore Hakuna, Hanjing Peng, Weixuan Chen, Yunfeng Cheng, Binghe Wang

Chemistry Faculty Publications and Presentations

Thiols are important molecules in the environment and in biological processes. Cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and hydrogen sulfide (H2S) play critical roles in a variety of physiological and pathological processes. The selective detection of thiols using reaction-based probes and sensors is very important in basic research and in disease diagnosis. This review focuses on the design of fluorescent and colorimetric probes and sensors for thiol detection. Thiol detection methods include probes and labeling agents based on nucleophilic addition and substitution, Michael addition, disulfide bond or Se-N bond cleavage, metal-sulfur interactions and more. Probes for H2S are based on …

Long-Term Trends In Air Temperature Distribution And Extremes, Growing Degree-Days, And Spring And Fall Frosts For Climate Impact Assessments On Agricultural Practices In Nebraska, Kari E. Skaggs, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Air temperature influences agricultural practices and production outcomes, making detailed quantifications of temperature changes necessary for potential positive and negative effects on agricultural management practices to be exploited or mitigated. Temperature trends of long-term data for five agricultural locations, ranging from the subhumid eastern to the semiarid western parts of Nebraska, were studied to determine local temperature changes and their potential effects on agricultural practices. The study quantified trends in annual and monthly average maximum and minimum air temperature (T_max and T_min), daily temperature range (DTR), total growing degree-days, extreme temperatures, growing-season dates and lengths, and …

Cognitive Architectures And Autonomy: Commentary And Response, Włodzisław Duch, Ah-Hwee Tan, Stan Franklin

Research Collection School Of Computing and Information Systems

This paper provides a very useful and promising analysis and comparison of current architectures of autonomous intelligent systems acting in real time and specific contexts, with all their constraints. The chosen issue of Cognitive Architectures and Autonomy is really a challenge for AI current projects and future research. I appreciate and endorse not only that challenge but many specific choices and claims; in particular: (i) that “autonomy” is a key concept for general intelligent systems; (ii) that “a core issue in cognitive architecture is the integration of cognitive processes ....”; (iii) the analysis of features and capabilities missing in current …

Nanobarcoding: Detecting Nanoparticles In Biological Samples Using In Situ Polymerase Chain Reaction, Trisha Eustaquio, James F. Leary

Birck and NCN Publications

Background: Determination of the fate of nanoparticles (NPs) in a biological system, or NP biodistribution, is critical in evaluating an NP formulation for nanomedicine. Current methods to determine NP biodistribution are greatly inadequate, due to their limited detection thresholds. Herein, proof of concept of a novel method for improved NP detection based on in situ polymerase chain reaction (ISPCR), coined "nanobarcoding," is demonstrated. Methods: Nanobarcoded superparamagnetic iron oxide nanoparticles (NB-SPIONs) were characterized by dynamic light scattering, zeta potential, and hyperspectral imaging - measurements. Cellular uptake of Cy5-labeled NB-SPIONs (Cy5-NB-SPIONs) was imaged by confocal microscopy. The feasibility of the nanobarcoding method …

Tyrosine Nitration Of Voltage-Dependent Anion Channels In Cardiac Ischemia-Reperfusion: Reduction By Peroxynitrite Scavenging, Meiying Yang, Amadou K.S. Camara, Bassam T. Wakim, Yifan Zhou, Ashish K. Gadicherla, Wai-Meng Kwok, David F. Stowe

Biomedical Engineering Faculty Research and Publications

Excess superoxide (O₂⁻) and nitric oxide (NO) forms peroxynitrite (ONOO⁻) during cardiac ischemia reperfusion (IR) injury, which in turn induces protein tyrosine nitration (tyr-N). Mitochondria are both a source of and target for ONOO⁻. Our aim was to identify specific mitochondrial proteins that display enhanced tyr-N after cardiac IR injury, and to explore whether inhibiting O₂⁻/ONOO⁻ during IR decreases mitochondrial protein tyr-N and consequently improves cardiac function. We show here that IR increased tyr-N of 35 and 15 kDa mitochondrial proteins using Western blot analysis with 3-nitrotyrosine antibody. Immunoprecipitation …

Predicting Domain Adaptivity: Redo Or Recycle?, Ting Yao, Chong-Wah Ngo, Shiai Zhu

Research Collection School Of Computing and Information Systems

Over the years, the academic researchers have contributed various visual concept classifiers. Nevertheless, given a new dataset, most researchers still prefer to develop large number of classifiers from scratch despite expensive labeling efforts and limited computing resources. A valid question is why not multimedia community “embrace the green” and recycle off-the-shelf classifiers for new dataset. The difficulty originates from the domain gap that there are many different factors that govern the development of a classifier and eventually drive its performance to emphasize certain aspects of dataset. Reapplying a classifier to an unseen dataset may end up GIGO (garbage in, garbage …

Docking System Design And Self-Assembly Control Of Distributed Swarm Flying Robots, Hongxing Wei, Ning Li, Yong Tao, Youdong Chen, Jindong Tan

Michigan Tech Publications, Part 1

This paper presents a novel docking system design and the distributed self-assembly control strategy for a Distributed Swarm Flying Robot (DSFR). The DSFR is a swarm robot comprising many identical robot modules that are able to move on the ground, dock with each other and fly coordinately once self-assembled into a robotic structure. A generalized adjacency matrix method is proposed to describe the configurations of robotic structures. Based on the docking system and the adjacency matrix, experiments are performed to demonstrate and verify the self-assembly control strategy.

Lonomics Atlas: A Tool To Explore Interconnected Ionomic, Genomic And Environmental Data, Eduard Dragut, Amgad Madkour, Mohamed Nabeel, Peter Baker, Mourad Ouzzani, David Salt

Cyber Center Publications

Ionomics Atlas facilitates access, analysis and interpretation of an existing large-scale heterogeneous dataset consisting of ionomic (elemental composition of an organism), ge- netic (heritable changes in the DNA of an organism) and ge- ographic information (geographic location, altitude, climate, soil properties, etc). Ionomics Atlas allows connections to be made between the genetic regulation of the ionome of plant populations and their landscape distribution, allowing scientists to investigate the role of natural ionomic variation in adaptation of populations to varied environmental conditions in the landscape. The goal of the Ionomics Atlas is twofold: (1) to allow both novice and expert users …

Functionalization Of In-Plane Photonic Microcantilever Arrays For Biosensing Applications, Stanley J. Ness

Theses and Dissertations

Microcantilevers have been investigated as high sensitivity, label free biosensors for approximately 15 years. In nearly all cases, a thin gold film deposited on the microcantilevers is used as an intermediate attachment layer because of the convenience of thiol-gold chemistry. Unfortunately, this attachment chemistry can be unstable when used with complex sample media such as blood plasma. The Nordin group at BYU has recently developed an all-silicon in-plane photonic microcantilever (PMCL) technology to serve as a platform for label-free biosensing. It has the advantage of being readily scalable to simultaneous readout of many PMCLs in array format, and allows integration …

In Situ Pm-Irras Studies Of A Floating Bilayer Lipid Membrane At Au(111) Electrode Surface, Su Zhangfei, Kycia Annia, Jay Leitch J., Lipkowski Jacek

Journal of Electrochemistry

In situ Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) was used to study the structure of a DMPC + cholesterol + GM1 floating bilayer lipid membrane (fBLM) at a Au(111) surface. 1-thio-beta-D-glucose (beta-Tg) was self-assembled onto the Au electrode to increase the overall hydrophilicity of the surface. The fBLM was deposited on the beta-Tg self-assembled monolayer (SAM) using a combination of Langmuir-Blodgett/Langmuir-Schaefer (LB/LS) techniques. The carbohydrate headgroups of the GM1 molecules were physically adsorbed to the beta-Tg SAM forming a water rich cushion between the fBLM and the modified gold substrate. The PM-IRRAS spectra indicate that the DMPC molecules within …

The Oxidation Of Hydrogen Peroxide On Nanostructured Rhodium Microelectrodes, N. Bartlett Philip, F. Esterle Thomas

Journal of Electrochemistry

Mesoporous Rh films were deposited onto platinum microelectrodes from the H₁ lyotropic liquid crystalline phase of C₁₂EO₈ (octaethyleneglycol monododecyl ether). The electrodes show well defined voltammetry for the oxidation and the reduction of hydrogen peroxide at low concentrations (<10 mmol•L-1) with excellent stability for operation at neutral pH. Based on the hysteresis in the current and the potential dependence the oxidation of hydrogen peroxide occurs through a CEE mechanism involving Rh(OH)₃ on the mesoporous Rh electrode surface. At higher hydrogen peroxide concentrations the current reaches a plateau that is due to either saturation of the binding sites for hydrogen peroxide or limitation of the reaction due to acidification of the solution within the pores. For the thin films (below 200 nm) the hydrogen peroxide calibration curves we …

Syngas Impurity Effects On Cell Growth, Enzymatic Activities And Ethanol Production Via Fermentation, Deshun Xu

Theses and Dissertations

A syngas compositional database with focus on trace impurities was established. For this work, ammonia (NH3) and benzene (C6H6) effects on cell growth, enzymatic activities of hydrogenase and alcohol dehydrogenase (ADH), and product formation were studied. NH3, after entering media, will be converted rapidly to NH4+, which will raise the total osmolarity of the media. NH3, as a common nutrient for the cell growth, is not the real culprit for cell growth inhibition. In essence, it is the high osmolarity resulting from the accumulation of NH4+ in the media which disrupts the normal regulation of the cells. It was concluded …

Lifecycle Energy Consumption Prediction Of Residential Buildings By Incorporating Longitudinal Uncertainties, Endong Wang, Zhigang Shen

Durham School of Architectural Engineering and Construction: Faculty Publications

Accurate prediction of buildings’ lifecycle energy consumption is a critical part in lifecycle assessment of residential buildings. Longitudinal variations in building conditions, weather conditions and building’s service life can cause significant deviation of the prediction from the real lifecycle energy consumption. The objective is to improve the accuracy of lifecycle energy consumption prediction by properly modelling the longitudinal variations in residential energy consumption model using Markov chain based stochastic approach. A stochastic Markov model considering longitudinal uncertainties in building condition, degree days, and service life is developed: 1) Building’s service life is estimated through Markov deterioration curve derived from actual …