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Slowing The Onset Of Hypoxia Increases Colony Forming Efficiency Of Connective Tissue Progenitor Cells In Vitro, Christopher M. Heylman, Tonya N. Caralla, Cynthia A. Boehm, Thomas E. Patterson, George F. Muschler

Biomedical Engineering

Background: Survival and colony formation by transplanted tissue derived connective tissue progenitor cells (CTPs) are thought to be important factors in the success of clinical tissue engineering strategies for bone regeneration. Transplantation of cells into defects larger than a few millimeters expose cells to a profoundly hypoxic environment. This study tested the hypothesis that delaying the onset of hypoxia will improve the survival and performance of CTPs in vitro.

Methods: To mimic declines seen in an avascular in vivo bone defect, colony forming efficiency by marrow derived nucleated cells was assessed under osteogenic conditions. Variation in the rate of …

Investigation On The Mobile Robot Navigation In An Unknown Environment, Ahmed S. Khusheef

Theses: Doctorates and Masters

Mobile robots could be used to search, find, and relocate objects in many types of manufacturing operations and environments. In this scenario, the target objects might reside with equal probability at any location in the environment and, therefore, the robot must navigate and search the whole area autonomously, and be equipped with specific sensors to detect objects. Novel challenges exist in developing a control system, which helps a mobile robot achieve such tasks, including constructing enhanced systems for navigation, and vision-based object recognition. The latter is important for undertaking the exploration task that requires an optimal object recognition technique.

In …

Characterising The Deformation Behaviour Of Human Tooth Enamel At The Microscale, Simona O'Brien

Theses: Doctorates and Masters

Enamel plays an important role in tooth function. Optimal combinations of composition and structure endow enamel with unique mechanical properties that remain largely unexplored. Specifically, more detailed understanding of the loadbearing ability of enamel is needed to mimic it synthetically and to design next generation biocomposite materials. This research investigates the variables that influence deformation behaviour of tooth enamel in relation to its hierarchical structure. Initially, a new method was developed for preparing flat, finely polished tooth samples that were maintained in their normal hydrated state for nanoindentation testing. In contrast to conventional methods, which commonly utilise either inappropriate or …

Subwavelength Surface Plasmons Based On Novel Structures And Metamaterials, Ruoxi Yang

Theses

With the rapid development of nanofabrication technology and powerful computational tools over the last decade, nanophotonics has enjoyed tremendous innovation and found wide applications in ultrahigh-speed data transmission, sensitive optical detection, manipulation of ultra-small objects, and visualization of nanoscale patterns. Surface plasmon-based photonics (or plasmonics) merges electronics and photonics at the nanoscale, creating the ability to combine the superior technical advantages of photonics and electronics on the same chip. Plasmonics focuses on the innovation of photonic devices by exploiting the optical property of metals. In particular, the oscillation of free electrons, when properly driven by electromagnetic waves, would form plasmon-polaritons …

Independent Component Analysis Enhancements For Source Separation In Immersive Audio Environments, Yue Zhao

Theses and Dissertations--Electrical and Computer Engineering

In immersive audio environments with distributed microphones, Independent Component Analysis (ICA) can be applied to uncover signals from a mixture of other signals and noise, such as in a cocktail party recording. ICA algorithms have been developed for instantaneous source mixtures and convolutional source mixtures. While ICA for instantaneous mixtures works when no delays exist between the signals in each mixture, distributed microphone recordings typically result various delays of the signals over the recorded channels. The convolutive ICA algorithm should account for delays; however, it requires many parameters to be set and often has stability issues. This thesis introduces the …

Evaluation Of Chemical Additives For The Separation And Recovery Of Bacteria From Food Matrices, Jennifer Leanne Frederick, Stephen P. Walker, Czarena L. Crofcheck, Melissa Newman, Frederick Payne

Biosystems and Agricultural Engineering Faculty Publications

The microbiological testing of foods is a well-established science. Due to the severity of foodborne pathogen illnesses, the widespread use and implementation of rapid detection methods in food testing labs is increasingly important. The first step for successful testing is sampling. Surfactants have been widely used in food microbiology, but there is not much, if any, published research about the use of fatty alcohols and chemical dispersants as aids in microbial separation and recovery. The microbial extraction efficiency of Escherichia coli K12 and Listeria innocua from three representative food matrices (hot dogs, spinach, and milk) was measured using chemical additives …

Application Of Nano-Imprint Lithography For Next Generation Carbon Nanotube-Based Devices, Svitlana Fialkova

Dissertations

This research report addresses the development of 3D carbon nanostructures that can provide unique capabilities for manufacturing carbon nanotube (CNT) electronic components, electrochemical probes, biosensors and tissue scaffolds. The shaped CNT arrays were grown on patterned catalytic substrate by chemical vapor deposition (CVD) method. The new fabrication process for catalyst patterning based on combination of nano-imprint lithography (NIL), magnetron sputtering and reactive etching techniques was proposed and studied. The optimal process parameters for each technique were evaluated. The catalyst was made by deposition of Fe and Co nanoparticles over alumina support layer on Si/SiO₂ substrate. The metal particles were …

Modeling Decision Making In Trauma Centers From The Standpoint Of Complex Adaptive Systems, Castanon De Policarpo

Dissertations

This research examines complex clinical decision-making processes in trauma center units of hospitals in terms of the impact of complexity on the medical team involved in the trauma event. The science of complex adaptive systems together with human judgment theories provide important concepts and tools for responding to health care challenges in this century and beyond. Clinical decision-makers in trauma centers are placed in urgent and anxious situations that are increasingly complex, making decision-making and problem-solving processes multifaceted. Under stressful circumstances, physicians must derive their decision-making schemas (―internal models‖ or ―mental models‖) without the benefits of judicious identification, evaluation, and/or …

Fitness Proportionate Niching: Harnessing The Power Of Evolutionary Algorithms For Evolving Cooperative Populations And Dynamic Clustering, Abrham Tibebu Workineh

Dissertations

Evolutionary algorithms work on the notion of "best fit will survive" criteria. This makes evolving a cooperative and diverse population in a competing environment via evolutionary algorithms a challenging task. Analogies to species interactions in natural ecological systems have been used to develop methods for maintaining diversity in a population. One such area that mimics species interactions in natural systems is the use of niching. Niching methods extend the application of EAs to areas that seeks to embrace multiple solutions to a given problem. The conventional fitness sharing technique has limitations when the multimodal fitness landscape has unequal peaks. Higher …

A Novel Approach To Intelligent Navigation Of A Mobile Robot In A Dynamic And Cluttered Indoor Environment, Daniel Opoku

Dissertations

The need and rationale for improved solutions to indoor robot navigation is increasingly driven by the influx of domestic and industrial mobile robots into the market. This research has developed and implemented a novel navigation technique for a mobile robot operating in a cluttered and dynamic indoor environment. It divides the indoor navigation problem into three distinct but interrelated parts, namely, localization, mapping and path planning. The localization part has been addressed using dead-reckoning (odometry). A least squares numerical approach has been used to calibrate the odometer parameters to minimize the effect of systematic errors on the performance, and an …

Enhanced Pvdf Film For Multi Energy Harvesting, Ranmunige Nadeeka Karunarathna

Electronic Theses and Dissertations

No abstract provided.

Autonomous Recovery Of Reconfigurable Logic Devices Using Priority Escalation Of Slack, Naveed Imran

Electronic Theses and Dissertations

Field Programmable Gate Array (FPGA) devices offer a suitable platform for survivable hardware architectures in mission-critical systems. In this dissertation, active dynamic redundancy-based fault-handling techniques are proposed which exploit the dynamic partial reconfiguration capability of SRAM-based FPGAs. Self-adaptation is realized by employing reconfiguration in detection, diagnosis, and recovery phases. To extend these concepts to semiconductor aging and process variation in the deep submicron era, resilient adaptable processing systems are sought to maintain quality and throughput requirements despite the vulnerabilities of the underlying computational devices. A new approach to autonomous fault-handling which addresses these goals is developed using only a uniplex …

Growth And Characterization Of Zno Based Semiconductor Materials And Devices, Ming Wei

Electronic Theses and Dissertations

Wide band gap semiconductors such as MgxZn1-xO represent an excellent choice for making optical photodetectors and emitters operating in the UV spectral region. High crystal and optical quality MgxZn1-xO thin films were grown epitaxially on c-plane sapphire substrates by plasma-assisted Molecular Beam Epitaxy. ZnO thin films with high crystalline quality, low defect and dislocation densities, and sub-nanometer surface roughness were achieved by applying a low temperature nucleation layer. The critical growth conditions were discussed to obtain a high quality film: the sequence of Zn and O sources for initial growth of nucleation layer, growth temperatures for both ZnO nucleation and …

Managing, Controlling And Improving The Treatment Of Produced Water Using The Six Sigma Methodology For The Iraqi Oil Fields, Maher T. Al-Shamkhani

Electronic Theses and Dissertations

Produced Water (PW) is the largest volume of waste that is normally generated during oil and gas production. It has large amounts of contaminants that can cause negative environmental and economic impacts. The management method for PW relies highly on types and concentrations of these contaminants, which are field dependent and can vary from one oil field to another. Produced water can be converted to fresh water if these contaminants are removed or reduced to the acceptable drinking water quality level. In addition, increasing oil production rate and reducing amounts of discharged harmful contaminants can be achieved by removing dissolved …

Real-Time In Vivo Imaging Of Size-Dependent Transport And Toxicity Of Gold Nanoparticles In Zebrafish Embryos Using Single Nanoparticle Plasmonic Spectroscopy, Lauren M. Browning, Tao Huang, Xiao-Hong Nancy Xu

Chemistry & Biochemistry Faculty Publications

Noble metal nanoparticles (NPs) show distinctive plasmonic optical properties and superior photostability, enabling them to serve as photostable multicoloured optical molecular probes and sensors for real-time in vivo imaging. To effectively study biological functions in vivo, it is essential that the NP probes are biocompatible and can be delivered into living organisms non-invasively. In this study, we have synthesized, purified and characterized stable (non-aggregated) gold (Au) NPs (86.2 +/- 10.8 nm). We have developed dark-field single NP plasmonic microscopy and spectroscopy to study their transport into early developing zebrafish embryos (cleavage stage) and their effects on embryonic development …

Proceedings Of The First International Conference On Smarandache Multispace & Multistructures, Florentin Smarandache, Linfan Mao

Branch Mathematics and Statistics Faculty and Staff Publications

In recent decades, Smarandache’s notions of multispace and multistructure were widely spread and have shown much importance in sciences around the world. Organizedby Prof.Linfan Mao, a professional conference on multispaces and multistructures, named the First International Conference on Smarandache Multispace and Multistructure was held in Beijing University of Civil Engineering and Architecture of P. R. China on June 28-30, 2013, which was announced by American Mathematical Society in advance.

Scandia And Ceria Stabilized Zirconia Based Electrolytes And Anodes For Intermediate Temperature Solid Oxide Fuel Cells: Manufacturing And Properties, Yan Chen

Electronic Theses and Dissertations

Mesoscale optical phenomena occur when light interacts with a number of different types of materials, such as biological and chemical systems and fabricated nanostructures. As a framework, mesoscale optics unifies the interpretations of the interaction of light with complex media when the outcome depends significantly upon the scale of the interaction. Most importantly, it guides the process of designing an optical sensing technique by focusing on the nature and amount of information that can be extracted from a measurement. Different aspects of mesoscale optics are addressed in this dissertation which led to the solution of a number of problems in …

Mesoscale Light-Matter Interactions, Kyle Douglass

Electronic Theses and Dissertations

Mesoscale optical phenomena occur when light interacts with a number of different types of materials, such as biological and chemical systems and fabricated nanostructures. As a framework, mesoscale optics unifies the interpretations of the interaction of light with complex media when the outcome depends significantly upon the scale of the interaction. Most importantly, it guides the process of designing an optical sensing technique by focusing on the nature and amount of information that can be extracted from a measurement. Different aspects of mesoscale optics are addressed in this dissertation which led to the solution of a number of problems in …

Fabrication Of Metallic Antenna Arrays Using Nanoimprint Lithography, Yu-Wei Lin

Electronic Theses and Dissertations

This Thesis describes the development of a cost-effective process for patterning nanoscale metal antenna arrays. Soft ultraviolet (UV) Nanoimprint Lithography (NIL) into bilayer resist was chosen since it enables repeatable large-scale replication of nanoscale patterns with good lift-off properties using a simple low-cost process. Nanofabrication often involves the use of Electron Beam Lithography (EBL) which enables the definition of nanoscale patterns on small sample regions, typically < 1 mm 2 . However its sequential nature makes the large scale production of nanostructured substrates using EBL cost-prohibitive. NIL is a pattern replication method that can reproduce nanoscale patterns in a parallel fashion, allowing the low-cost and rapid production of a large number of nanopatterned samples based on a single nanostructured master mold. Standard NIL replicates patterns by pressing a nanostructured hard mold into a soft resist layer on a substrate resulting in exposed substrate regions, followed by an optional Reactive Ion Etching (RIE) step and the subsequent deposition of e.g. metal onto the exposed substrate area. However, non-vertical sidewalls of the features in the resist layer resulting from an imperfect hard mold, from reflow of the resist layer, or from isotropic etching in the RIE step iii may cause imperfect lift-off. To overcome this problem, a bilayer resist method can be used. Using stacked resist layers with different etch rates, undercut structures can be obtained after the RIE step, allowing for easy lift-off even when using a mold with non-vertical sidewalls. Experiments were carried out using a nanostructured negative SiO2 master mold. Various material combinations and processing methods were explored. The negative master mold was transferred to a positive soft mold, leaving the original master mold unaltered. The soft mold consisted of a 5 m thick top Poly(methyl methacrylate) (PMMA), or Polyvinyl alcohol (PVA) layer, a 1.5 mm thick Polydimethylsiloxane (PDMS) buffer layer, and a glass supporting substrate. The soft mold was pressed into a bilayer of 300 nm PMMA and 350 nm of silicon based UV-curable resist that was spin-coated onto a glass slide, and cured using UV radiation. The imprinted patterns were etched using RIE, exposing the substrate, followed by metal deposition and lift-off. The experiments show that the use of soft molds enables successful pattern transfer even in the presence of small dust particles between the mold and the resist layer. Feature sizes down to 280 nm were replicated successfully

Vision-Based Sensing And Optimal Control For Low-Cost And Small Satellite Platforms, Bradley Sease

Electronic Theses and Dissertations

Current trends in spacecraft are leading to smaller, more inexpensive options whenever possible. This shift has been primarily pursued for the opportunity to open a new frontier for technologies with a small financial obligation. Limited power, processing, pointing, and communication capabilities are all common issues which must be considered when miniaturizing systems and implementing low-cost components. This thesis addresses some of these concerns by applying two methods, in attitude estimation and control. Additionally, these methods are not restricted to only small, inexpensive satellites, but offer a benefit to large-scale spacecraft as well. First, star cameras are examined for the tendency …

The Use Of Complexity Theory And Strange Attractors To Understand And Explain Information System Development, Arthur P. Tomasino

2013

In spite of the best efforts of researchers and practitioners, Information Systems (IS) developers are having problems "getting it right". IS developments are challenged by the emergence of unanticipated IS characteristics undermining managers ability to predict and manage IS change. Because IS are complex, development formulas, best practices or development guides simply will not work. The difficulties in these system developments stem from the complexity of IS arising from the inter-relationship, interaction, and interconnectivity of the elements in the system and its environment. This research uses complexity concepts to help solve the problem with IS development and explain why so …

Biological Control Of Manganese In Water Supplies In The Presence Of Humic Acids, Michael S. Snyder

Theses and Dissertations--Civil Engineering

The main objective of this study was to improve our understanding of biological filtration (biofilm type) treatment for manganese (Mn) removal in drinking water. Biological filtration treatment involves biofilms of Mn(II)-oxidizing microorganisms attached to solid filter material that remove and immobilize dissolved Mn(II) in raw water by conversion to black MnO_2(s) precipitates. Mn-biological filtration is an emerging green technology that can serve as an alternative to conventional physicochemical treatments but its full potential is hindered by various factors. These include lack of understanding the: (1) optimal removal conditions for Mn, (2) mechanisms for Mn releases of the accumulated Mn …

Noninvasive Near-Infrared Diffuse Optical Monitoring Of Cerebral Hemodynamics And Autoregulation, Ran Cheng

Theses and Dissertations--Biomedical Engineering

Many cerebral diseases are associated with abnormal cerebral hemodynamics and impaired cerebral autoregulation (CA). CA is a mechanism to maintain cerebral blood flow (CBF) stable when mean arterial pressure (MAP) fluctuates. Evaluating these abnormalities requires direct measurements of cerebral hemodynamics and MAP. Several near-infrared diffuse optical instruments have been developed in our laboratory for hemodynamic measurements including near-infrared spectroscopy (NIRS), diffuse correlation spectroscopy (DCS), hybrid NIRS/DCS, and dual-wavelength DCS flow-oximeter. We utilized these noninvasive technologies to quantify CBF and cerebral oxygenation in different populations under different physiological conditions/manipulations. A commercial finger plethysmograph was used to continuously monitor MAP. For investigating …

Water Quality Sensor Placement Guidance For Small Water Distribution Systems, Stacey L. Schal

Theses and Dissertations--Civil Engineering

Water distribution systems are vulnerable to intentional, along with accidental, contamination of the water supply. Contamination warning systems (CWS) are strategies to lessen the effects of contamination by delivering early indication of an event. Online quality monitoring, a network of sensors that can assess water quality and alert an operator of contamination, is a critical component of CWS, but utilities are faced with the decision of what locations are optimal for deployment of sensors. A sensor placement algorithm was developed and implemented in a commercial network distribution model (i.e. KYPIPE) to aid small utilities in sensor placement. The developed sensor …

Investigation Of Media Ingredients And Water Sources For Algae Co2 Capture At Different Scales To Demonstrate The Correlations Between Lab-Scale And Large-Scale Growth, Tabitha Graham

Theses and Dissertations--Biosystems and Agricultural Engineering

As energy use increases globally the environmental burdens increase alike. Many accusations have been made that carbon dioxide is a culprit of climate change. The University of Kentucky and Duke Energy Power have partnered to test carbon capture technology in a large scale project. To this end, the objective of this thesis is to investigate potential water media sources and nutrient sources at different volume scales for algae cultivation to help create a more environmentally viable and economically feasible solution. This work will conduct a life cycle assessment of water media sources and the effects of the inputs and outputs …

Alkaline Hydrogen Peroxide Pretreatment For Its Use In An On-Farm Bioprocessing Facility, Mary Kathryn Gray

Theses and Dissertations--Biosystems and Agricultural Engineering

Pretreatment is an essential step in biofuel production from lignocellulose. Disruption of the lignin structure gives enzymes and fermentation organisms access to long chains of cellulose and hemicellulose. For this project’s purposes, the pretreatment must work within the framework of an on-farm butanol bioprocessing facility. Alkaline hydrogen peroxide (AHP) is a delignification method that potentially provides several advantages. At the alkaline pH, powerful hydroxyl radicals are formed; which attack lignin. The objectives of this study were to determine if AHP removes substantial lignin for the feedstocks, corn stover, wheat straw, switchgrass and miscanthus, and to determine if AHP acts as …

Sodium Hydroxide Pretreatment Of Corn Stover And Subsequent Enzymatic Hydrolysis: An Investigation Of Yields, Kinetic Modeling And Glucose Recovery, Alicia Modenbach

Theses and Dissertations--Biosystems and Agricultural Engineering

Many aspects associated with conversion of lignocellulose to biofuels and other valuable products have been investigated to develop the most effective processes for biorefineries. The goal of this research was to improve the efficiency and effectiveness of the lignocellulose conversion process by achieving a more basic understanding of pretreatment and enzymatic hydrolysis at high solids, including kinetic modeling and separation and recovery of glucose.

Effects of NaOH pretreatment conditions on saccharide yields from enzymatic hydrolysis were characterized in low- and high-solids systems. Factors associated with pretreatment and hydrolysis were investigated, including duration of pretreatment at different temperatures and NaOH loadings, …

Hydrologic Characterization Of A Rain Garden Mitigating Stormwater Runoff From A Commercial Area, John T. Mcmaine

Theses and Dissertations--Biosystems and Agricultural Engineering

Impervious surfaces such as roads, sidewalks, and roofs increase the volume of runoff generated in a watershed. Traditional stormwater management techniques emphasize conveyance of runoff away from impervious surfaces in order to reduce flooding. Rain gardens are becoming popular as a different means to manage stormwater in such a way that runoff is captured and infiltrated onsite rather than conveyed offsite. A stormwater management system consisting of a rainwater harvest system, rain garden, and infiltration chamber was built at the Coca-Cola Refreshments USA, Inc. distribution center in Lexington, Kentucky during the fall of 2011. Precipitation, inflow, and water level were …

Nutrients Recycling Strategy For Microalgae-Based Co2 Mitigation System, Xinyi E

Theses and Dissertations--Biosystems and Agricultural Engineering

Coal-fired electricity production is the major emitter of CO₂ and other greenhouse gases including NO_x and SO_x. Microalgae-based CO₂ mitigation systems have been proposed to reduce the net CO₂ emission from coal-fired power plants. This study focused on developing an optimum culture media and exploring the possibilities for recycling nutrients, which were added as commercial mineralized chemicals at the beginning of cultivation.

In order to release the nutrients embedded in the cells so that they can be used as a nutrient source for new cells, Scenedesmus biomass was digested by anaerobic bacteria. Results showed …

Characterizing The Variability In Respirable Dust Exposure Using Johnson Transformation And Re-Examining 2010 Proposed Changes To The U.S. Underground Coal Mine Dust Standard, Al I. Khan

Theses and Dissertations--Mining Engineering

Coal workers’ pneumoconiosis (CWP), commonly referred to as black lung, is a chronic lung disease that results from the inhalation and deposition of coal dust in the lungs. While this disease continues to afflict coal miners, its prevalence has steadily declined over three decades since 1970. Based on a voluntary X-ray surveillance program, conducted by the National Institute for Occupational Safety and Health (NIOSH), this downward trend, however, ended in 2000 and has actually begun to rise. The Mine Safety and Health Administration (MSHA) instituted a Comprehensive Initiative to “End Black Lung” to combat the reported upturn in black …