Digital Commons Network^™

Effect Of Architecture And Porosity On Mechanical Properties Of Borate Glass Scaffolds Made By Selective Laser Sintering, Krishna C. R. Kolan, Ming-Chuan Leu, Greg Hilmas, Taylor Comte

Mechanical and Aerospace Engineering Faculty Research & Creative Works

The porosity and architecture of bone scaffolds, intended for use in bone repair or replacement, are two of the most important parameters in the field of bone tissue engineering. The two parameters not only affect the mechanical properties of the scaffolds but also aid in determining the amount of bone regeneration after implantation. Scaffolds with five different architectures and four porosity levels were fabricated using borate bioactive glass (13-93B3) using the selective laser sintering (SLS) process. The pore size of the scaffolds varied from 400 to 1300 μm. The compressive strength of the scaffolds varied from 1.7 to 15.5 MPa …

Third World Water Filtration System Implementation, Christopher Welton, Perry Carter

Journal of Undergraduate Research

Approximately 70 million people in rural India do not have access to clean water. Chemicals such as cesium, arsenic, and others contaminate India’s drinking water, rendering it unsafe. Biological contaminates pose increased health risks to those drinking the water. If a water source is bad, large populations need to relocate to find a better source of water. Creating an economic water filtration system capable of being obtained through a micro-loan is beginning alleviate this problem. By creating an affordable and quality system, clean water is becoming a reality for the world’s poor, especially in Third World countries. Through my efforts …

Bridging The Gap Between Simulation And Experiment With Multi-State Protein Folding, Jacob Lewis, Dr. Thomas Knotts

Journal of Undergraduate Research

Proteins are made up of extremely long chains of amino acids. Depending on it constituent amino acids, proteins fold in many 3-dimensional patterns to facilitate various biochemical processes such as catalyzing chemical reactions which allow us to live. How proteins fold is thought to be the “holy grail” of biochemistry research and, as of yet, no one has been able to predict exactly how a given sequence of amino acids will fold a priori. Protein folding has been probed in experiment and simulated on computers, but there are discrepancies between the two methods. Naturally, the experimental results are what computer …

Simultaneous Decision Making Of Optimal Toll Levels And Locations In A Multi-Class Network Equilibrium: Genetic Algorithm Approach, Zegeye K. Gurmu

Civil Engineering Theses

The purpose of this thesis is to explore bi-level genetic algorithm (GA) based optimization models to make decisions simultaneously for the second-best optimal toll locations and toll levels. The upper-level subprogram is to minimize the total travel time (system cost). The lower-level subprogram is a user equilibrium problem where all users try to find the route that minimizes their own travel cost (or time). The demand is assumed to be fixed and given a priority. First, two different versions of GA based solution procedures are developed and applied to an example Sioux Falls network assuming homogeneous road users in the …

The Trm2h : Detecting Protein-Protein Interactions By Engineering The Cell As A Biosensor, Elyse Shimomura, Paulina Perezalonso

Bioengineering Senior Theses

Protein-protein interactions regulate key cellular functions and cell signaling pathways in the body; biological systems can be disrupted by protein interactions or lack of interactions that cause cell dysfunction and can lead to disease or illness. However, studying these interactions is complex and difficult to quantify. In this project, we examine the interaction of two putative cancer biomarkers, Bax and PDCDS, using the tetracycline repressor-based mammalian two-hybrid system (trM2H). This in vivo system engineers the cell as a biosensor using methods of DNA cloning and mammalian cell transfections that easily detects and quantities the interaction of two proteins by the …

Propagation Of Thermo-Mechanical Waves In Deforming Nonlinear Viscoelastic Bodies, Lili Zhang

Department of Engineering Mechanics: Dissertations, Theses, and Student Research

This work studies the propagation of thermo-mechanical disturbances in bodies made of viscoelastic materials that might already be loaded such that they are undergoing large inhomogeneous time varying deformations. In the process of this study we develop the general equations governing the thermo-mechanical motion of such disturbances and ones for internally constrained systems, provide the general structure of the solution, match the solution to existing results for the special case of time harmonic plane waves in elastic bodies and in viscoelastic bodies under constant homogenous loading, and consider some special applications.

The results of this work should have applications in …

An Applied Numerical Simulation Of Entrained-Flow Coal Gasification With Improved Sub-Models, Xijia Lu

LSU New Orleans Theses and Dissertations

The United States holds the world's largest estimated reserves of coal and is also a net exporter of it. Coal gasification provides a cleaner way to utilize coal than directly burning it. Gasification is an incomplete oxidation process that converts various carbon-based feedstocks into clean synthetic gas (syngas), which can be used to produce electricity and mechanical power with significantly reduced emissions. Syngas can also be used as feedstock for making chemicals and various materials.

A Computational Fluid Dynamics (CFD) scheme has been used to simulate the gasification process for many years. However, many sub-models still need to be developed …

Spectral Discrimination Of Breast Pathologies In Situ Using Spatial Frequency Domain Imaging, Ashley M. Laughney, Venkataramanan Krishnaswamy, Elizabeth J. Rizzo, Mary C. Schwab, Richard J. Barth, David J. Cuccia, Bruce J. Tromberg, Keith D. Paulsen, Brian W. Pogue, Wendy A. Wells

Dartmouth Scholarship

Introduction: Nationally, 25% to 50% of patients undergoing lumpectomy for local management of breast cancer require a secondary excision because of the persistence of residual tumor. Intraoperative assessment of specimen margins by frozen-section analysis is not widely adopted in breast-conserving surgery. Here, a new approach to wide-field optical imaging of breast pathology in situ was tested to determine whether the system could accurately discriminate cancer from benign tissues before routine pathological processing.

Characteristics Of Vacuum Freeze Drying With Utilization Of Internal Cooling And Condenser Waste Heat For Sublimation, Muhammad Idrus Alhamid, Nasruddin Yusuf, Indra Teuku Meurah Mahlia, Engkos Ahmad Kosasih, Muhamad Yulianto, Rio Ricardi

Makara Journal of Technology

Vacuum freeze drying is an excellent drying method, but it is very energy-intensive because a relatively long drying time is required. This research investigates the utilization of condenser waste heat for sublimation as a way of accelerating the drying rate. In addition, it also investigates the effect of internal cooling combined with vacuum cooling in the pressure reduction process. Jelly fish tentacles were used as the specimen, with different configurations for condenser heat waste and internal cooling valve opening. The results show that heating with condenser heat waste can accelerate the drying rate up to 0.0035 kg/m2.s. In addition, pre-freezing …

Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, Emmy O. Trieu, Michael Flynn, Rocco Mancinelli

STAR Program Research Presentations

In deep space missions, maintaining life support is of the utmost priority. In such a closed system, human waste must be stored and treated. Simulated feces (ersatz) will be inoculated with microbes representing normal fecal flora, mixed with urine brine and shredded refuse of products typically used in space missions. Composting methods often use alternating layers of waste with scraps of carboniferous materials (finely shredded refuse). By preparing membrane bags with a homogenized ersatz and carboniferous refuse mixture and membrane bags with alternating layers of ersatz and carboniferous refuse, it may be possible to monitor anaerobic thermophillic digestion of the …

Creating A Package In R, Brit Schneiders, Eric Archer

STAR Program Research Presentations

In a time of increasingly efficient technology and data production, scientists are producing data faster than it can be analyzed. Therefore, user accessibility to data analysis is becoming more and more critical. In general, researchers have a set of raw data and want an efficient means to their final analysis. A package serves as that means by creating a set of functions and making them accessible to the user. Often, a user has a small piece of code to run (a single R script, for example), and that script requires the use of certain functions, which are contained in a …

Design, Fabrication And Test Of An Operationally Responsive Aircraft With Niirs Evaluated Imager, Colin Burt

Master's Theses

Unmanned Aerial Systems (UAS) are a growing asset. Currently UAS are on the cutting edge with resources being spent developing the capabilities mostly for military use. This project is intended to create a system for non-defense customers. Specifically, the Operationally Responsive Aircraft (ORA) will appeal to academic institutions, individual consumers, future customers new to the UAS industry, as well as anybody trying to get airtime for custom sensors.

The system developed in this project utilizes dual aluminum external payload bays attached to a ParkZone Radian aircraft. Each external payload bay can contain approximately 500 $\text{cm}^3$, with a height and width …

Interleukin Expression After Injury And The Effects Of Interleukin-1 Receptor Antagonist, Connie S. Chamberlain, Ellen M. Leiferman, Kayt E. Frisch, Stacey L. Brickson, William L. Murphy, Geoffrey S. Baer

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

Ligament and tendon repair involves a complex series of coordinated events orchestrated by various cell types, cytokines and other factors. The repair process extends months to years and results in scar tissue mechanically inferior to native tissue. This is in clear contrast to tissue "regeneration" which would recapitulate the native tissue. Numerous approaches to stimulate a regenerative scenario have been attempted, including tissue-engineering, non-steroidal anti-inflammatories, ultrasonic, or electrical stimulation, but none have resulted in complete regeneration. In pursuit of a more regenerative outcome, it is important to understand the fundamentals of the normal healing process. Previous work from our lab …

Moving Domain Computational Fluid Dynamics To Interface With An Embryonic Model Of Cardiac Morphogenesis, Juhyun Lee, Mahdi Esmaily Moghadam, Ethan Kung, Hung Cao, Tyler Beebe, Yury Miller, Beth L. Roman, Ching-Ling Lien, Neil C. Chi, Alison L. Marsden, Tzung K. Hsiai

Publications

Peristaltic contraction of the embryonic heart tube produces time- and spatial-varying wall shear stress (WSS) and pressure gradients (∇P) across the atrioventricular (AV) canal. Zebrafish (Danio rerio) are a genetically tractable system to investigate cardiac morphogenesis. The use of Tg(fli1a:EGFP)y1 transgenic embryos allowed for delineation and two-dimensional reconstruction of the endocardium. This time-varying wall motion was then prescribed in a two-dimensional moving domain computational fluid dynamics (CFD) model, providing new insights into spatial and temporal variations in WSS and ∇P during cardiac development. The CFD simulations were validated with particle image …

Scitech Book News Reviews, Susan Fingerman

Sci-Tech News

No abstract provided.

A Humidity-Sensitive Hydrogel-Bacillus Spore Composite For Micropatterning Of Biomolecular Gradients, Richard L. Gieseck, Bin-Da Chan, Cagri A. Savran

Birck and NCN Publications

A composite material consisting of Bacillus subtilis spores suspended in a humidity sensitive hydrogel can be used to pattern biomolecules in different concentrations directly onto glass surfaces using a mechanical micromanipulator. By altering the relative humidity surrounding the composite gel during deposition, surface concentration of patterned biomolecules can be controlled and varied to create user-defined, biomolecular surface concentrations. (C) 2013 AIP Publishing LLC.

2013 Tsu Undergraduate Research Program, David Owerbach

Office of Research Institutional Research and Scholarship

No abstract provided.

The Effects Of Variation In Collagen D-Spacing On Compact Bone Viscoelasticity: A Finite Element Analysis, Miguel A. Mendoza

Master's Theses

The D-spacing that is characteristic of collagen and its structural arrangement was previously thought to be a constant value. Much research is revealing that it is actually a distribution of values in biological tissues. Recent ovine experimentation has also shown that the D-spacing distribution is significantly altered following estrogen depletion. While ewes contain some major biological differences between their human counterparts, they are an economical and robust large animal model for postmenopausal osteoporosis. So, the exploration of the possible implications that D-spacing has on the mechanical properties of the whole bone utilizing animal models and computational methods is warranted. Six …

Smarter Neat Nets, Ryan Swords Dehaven

Master's Theses

This paper discusses a modification to improve usability and functionality of a ge- netic neural net algorithm called NEAT (NeuroEvolution of Augmenting Topolo- gies). The modification aims to accomplish its goal by automatically changing parameters used by the algorithm with little input from a user. The advan- tage of the modification is to reduce the guesswork needed to setup a successful experiment with NEAT that produces a usable Artificial Intelligence (AI). The modified algorithm is tested against the unmodified NEAT with several different setups and the results are discussed. The algorithm shows strengths in some areas but can increase the …

Implantable Biosensors For Physiologic Status Monitoring During Hemorrhage, Christian Kotanen

All Dissertations

Trauma diagnostics and management are major aims of research for implantable amperometric enzyme biosensor technology. Biosensors are capable of monitoring metabolic variables in a minimally invasive manner and have great potential to augment current wireless vital sign monitoring technologies in order to make a more robust physiologic status monitoring platform. The dual responsive Electrochemical Cell-on-a-Chip Microdisc Electrode Array (ECC MDEA 5037) is a recently developed electrochemical transducer for use in a wireless, implantable biosensor system for the continuous measurement of interstitial glucose and lactate. Hyperglycemia arising from insulin resistance and hyperlactatemia arising from anaerobic metabolism both occur following trauma and …

Pan Nanofibers And Nanofiber Reinforced Composites, Cheng Ren

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Nanomaterials play an important role in the development of nanotechnology. They possess unique mechanical, physical, and chemical properties coupled with small size and ultrahigh surface area that can provide critical advantages for applications. Continuous nanofibers attract special interest due to their dual nano-macro nature and ability to bridge scales. Nanofibers are being considered for a broad range of applications spanning advanced filters, separation membranes, ultrasensitive sensors, micro/nano actuators, nanoprobes, tissue engineering scaffolds, protective and smart closing, and multifunctional composites. Most of these applications require certain mechanical properties and robustness. However, the literature on the mechanical behavior of nanofibers and their …

Controlling Bacterial Persister Cells And Biofilms By Synthetic Brominated Furanones, Jiachuan Pan

Biomedical and Chemical Engineering - Dissertations

Bacteria are well known to obtain tolerance to antibiotics by forming multicellular structures, known as biofilms, and by entering dormancy and forming persister cells. Both mechanisms allow bacteria to tolerate antibiotics at concentrations hundreds to thousands of times higher than the lethal dose for regular planktonic cells of the same genotype. Persister formation increases in biofilms; thus, effective control of persister cells, especially those in biofilms is critically important to infection control. Over the past decades, a bacterial signaling system based on cell density, named quorum sensing (QS), has been found to regulate biofilm formation and, in Pseudomonas aeruginosa, the …

Extraction Of Antioxidants From Animal Blood And Its Potential Application As A Pet Food Preservative, Chengyi Tu

All Theses

Nowadays, more and more people are having pets as members of their family. To the year of 2012, there are 78.2 million dogs and 86.4 million cats owned in the U.S according to the report of the Humane Society of the U.S. The pet food industry as a result has been prosperous, with an estimated market size of $21 billion in the year of 2013. However, there is a common problem for the industry - fat rancidification. Pet foods usually contain relatively high levels of fat, which, if not well protected, are prone to oxidation and generate unfavorable products including …

Fabrication And Characterization Of Radio-Frequency Sensors For Liquid Material Detection, Yuxi He

All Theses

This thesis presents a series of studies on fabrication and characterization of radio-frequency (RF) sensors. In the light of Electromagnetics and Transmission Line Theory, we designed multiple RF sensors with different detection capability emphasis and used them to detect various materials, especially liquid samples and materials in solutions such as dielectric thin films, confined liquids, red blood cells, and malarial pigments (hemozoin). Most sensors were fabricated under clean room environment following the standard process protocols. Proper process developments were also made to achieve special structures and functionalities of our novel sensors. Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) …

Particle Electrophoresis And Magnetophoresis In Microchannels, Litao Liang

All Theses

In current pharmaceutical and biotechnology industries with clinical applications, an increased demand for flow control and cell manipulation on the micrometer scale has emerged. Electrokinetic, magnetic and many other physics fields have been exploited to meet this demand. However, due to the requirement for sophisticated micro-structures and the interference of the increasing significance of many `trivial' physics properties (surface potential, permittivity, etc.) at the smaller scale, most applications encounter poor maneuverability and high operation/fabrication complexity issues. Very few attempts have been made to bypass these requirements while maintaining the same control and efficiency. This thesis research investigates the fundamental behaviors …

Topology Optimization Using A Level Set Penalization With Constrained Topology Features, Luis Fernandez

All Theses

Topology optimization techniques have been applied to structural design problems in order to determine the best material distribution in a given domain. The topology optimization problem is ill-posed because optimal designs tend to have infinite number of holes. In order to regularize this problem, a geometrical constraint, for instance the perimeter of the design (i.e., the measure of the boundary of the solid region, length in 2D problems or the surface area in 3D problems) is usually imposed. In this thesis, a novel methodology to solve the topology optimization problem with a constraint on the number of holes is proposed. …

Statistical Regression Methods For Gpgpu Design Space Exploration, Nimisha Raut

All Theses

General Purpose Graphics Processing Units (GPGPUs) have leveraged the performance and power efficiency of today's heterogeneous systems to usher in a new era of innovation in high-performance scientific computing. These systems can offer significantly high performance for massively parallel applications; however, their resources may be wasted due to inefficient tuning strategies. Previous application tuning studies pre-dominantly employ low-level, architecture specific tuning which can make the performance modeling task difficult and less generic. In this research, we explore the GPGPU design space featuring the memory hierarchy for application tuning using regression-based performance prediction framework and rank the design space based on …

Comparison Of Methods To Evaluate Chitosan As An Antimicrobial Against Listeria Spp. Cultures, Scott Hartley

All Theses

The antimicrobial properties of a chitosan-coated vacuum-packaged pouch were tested against Listeria monocytogenes (LM) inoculated on frankfurters. A 1.5% chitosan solution was successfully coated onto a corona-treated poly/nylon based film and then heat-sealed to form a pouch. An initial 5 log CFU/mL population of LM was used to inoculate a frankfurter in the pouch, which was then vacuum-sealed and stored at 4¡ and 13¡ C for a period of 8 weeks, with bacteria enumeration performed on weekly intervals. An uncoated pouch was tested as a control. It was found that the chitosan-coated pouches did not exhibit any antimicrobial properties against …

Continuum Robotic Surface: Forward Kinematic Analysis And Implementation, Jessica Merino

All Theses

This thesis presents a new class of biologically inspired robots: continuum robotic surfaces. This work is fueled by the question: can the interaction between robot and environment be advanced with “programmable surfaces in space?” The novelty of continuum robotic surfaces lies in their ability to be actively controlled and reconfigured in what we believe is the current “missing dimension” in robot movements — two–dimensional space. We believe that such surfaces will lend themselves to more complex applications. However, to effectively deploy such surfaces for these complex applications, kinematic models will be necessary to plan and control desired configurations. The forward …

Biological Production Of Succinic Acid Using A Cull Peach Medium, Janani Krishnakumar

All Theses

Biological production of succinic acid from a cull peach based medium was studied using Escherichia coli AFP 184 in a dual-phase batch process including an aerobic growth phase and an anaerobic production phase. Experiments were performed to study the effects of corn steep liquor supplement and exogenous hydrogen supply. About one half of the sucrose in the medium was converted to fermentable sugars glucose and fructose during sterilization. The remaining sucrose was hydrolyzed with invertase. The viscosity of the medium was reduced from 350 cP to 50 cP to allow efficient oxygen transfer by a commercial enzyme product. In the …