Digital Commons Network^™

Degradation Of Cobalt Chrome Molybdenum Hip And Knee Implants In Vivo And In Vitro, Peter William Kurtz

All Dissertations

Cobalt Chrome Molybdenum (CoCrMo) remains a popular material choice for femoral condyles in total knee arthroplasty and for acetabular liners in dual mobility total hip designs. CoCrMo has been phased out of conventional THA designs due to its correlation with adverse effects such as ALTR and ALVAL. It is less understood if the mechanisms (i.e. abrasive wear to the surface of the implant) that release metal ions into the surrounding tissue occur at similar rates as seen in conventional THA. Additionally, it is unknown what effect the secondary damage modes such as electrocautery damage and adhesive wear have on these …

Particle Migration In Non-Newtonian Fluid Flows Through Curved Microchannels, Sanskruti Ramesh Raut

All Dissertations

Innovations in microfabrication over the past three decades have enabled the development of microfluidic devices with intricate channel geometry. Microfluidic platforms offer controlled manipulation of fluids and particles by leveraging the internally induced and/or externally imposed force field(s). The demand for compact microchannel geometry has turned the attention toward curved microchannels with significantly reduced footprints. However, most of the previous studies in curved microchannels have been limited to particle migration in Newtonian fluids. The influence of fluid rheology has remained largely unexplored.

In the first part of this dissertation, we present a systematic experimental study on the influences of fluid …

Universal Systems Simulation Via Constraint Hypergraphs With Applications To Digital Twins, John Morris

All Dissertations

The characterization of systems encompasses a variety of modeling frameworks designed to capture specific behaviors and components of various system domains. Whatever the framework, the core elements of a system representation are the information of the system and a description of how that information is related. The relations in deterministic systems are functions, which, when composed to form executable processes, can be used to simulate system data. A declarative modeling framework is one that encodes mechanisms for preparing these simulations within the model structure, allowing an external agent to form the execution processes required for a given context. To date, …

Developing A Metabolic Engineering Chassis From A Metabolically Versatile Organism, Rhodopseudomonas Palustris Cga009, Mark William Kathol

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Metabolic engineering has made it possible to develop biological processes that were once considered economically unfeasible or uncompetitive. This field has enabled both a robust niche market, whereby products, such as pharmaceutical substances like monoclonal antibodies, are produced exclusively through biological means, as well as an emerging commodity production market. Nonetheless, significant challenges remain in harnessing the unique metabolic capabilities of genetically non-tractable organisms. This dissertation focuses on Rhodopseudomonas palustris CGA009 as a potential chassis for metabolic engineering. To address the issue of genetic intractability, a synthetic biology toolkit was developed to facilitate the expression of heterologous proteins in R …

In-Field Tractor Operational Load Profile Generation In Support Of Advanced Tractor Testing In Mixedmode Power, Andrew Donesky

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation addresses the need to better characterize real-world tractor power requirements across drawbar, power take-off (PTO), and hydraulic modes to support more representative tractor testing. Conventional testing protocols, such as OECD Code 2, emphasize steady-state performance under controlled high-load conditions, which do not reflect the mixed and dynamic demands of modern field operations. To address this gap, a Tractor Instrumentation System (TIS) was developed, validated, and deployed to collect high-resolution, mixed-mode power data during planting, anhydrous ammonia application, and grain cart operations. The TIS integrates physical sensors, including custom load pins, hydraulic pressure/flow sensors, and a redesigned PTO torque …

An Integrated Modeling Framework To Evaluate Circularity Of The Corn–Water–Ethanol–Beef Nexus, Heydi Han

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation examines the transition from conventional linear agricultural and food bioenergy systems toward more circular, resilient, and resource-efficient configurations. Prior research indicates that spatial and temporal variability in soil, weather, animal biological responses, and management decisions strongly influences productivity, nutrient-use efficiency, and profitability. Studies have also shown that a diversified circular system can buffer these sources of variability, improving stability and adaptive capacity. Building on these insights, the central focus of this work is to develop an integrated modeling framework to evaluate circularity within the Corn-Water-Ethanol-Beef (CWEB) nexus and explore how climate variability and management choices shape system-wide performance. …

Synthesis And Study Of Stable Organic Radicals For Mri Contrast Agents And New Materials, Sabina Dhakal

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The first part of this dissertation focuses on the design, synthesis, and characterization of a thermally robust S =1/2 phototetrazolinyl monoradical and the development of synthetic methodologies for a high-spin (S = 1) phototetrazolinyl diradical. Electrochemical studies of the phototetrazolium cation (precursor to the monoradical) revealed a remarkably narrow electrochemical band gap (Ecell ≈ 0.82 V), suggesting promising electrical conductivity. Thermal analysis of the monoradical demonstrated excellent stability, with the onset of decomposition at 232 °C. Building on the excellent thermal stability and promising properties for electrical conductivity of the monoradical, we developed two condensation-based synthetic routes that provide viable …

Large-Scale Experimental Study On The Impact Of Air Cleaning & Ventilation On Indoor Air Quality And Student Illness-Related Absenteeism, Daud Nosham

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This large-scale randomized experimental field study investigated the effect of portable air purifiers (PAPs) on classroom’s air quality and student’s illness related absenteeism. PAPs were installed in 317 classrooms from various school districts located in Eastern Nebraska. The classrooms were randomly assigned into four conditions: three treatment conditions (T1, T2 & T3) and one control condition (device without any filter). T1 was equipped with HEPA filters, T2 added Activated Carbon (AC) layer to HEPA, and T3 further integrated Germicidal Ultraviolet (GUV) alongside HEPA and AC filters. The measured variables were carbon dioxide (CO2), fine particles (PN2.5) and coarse particles (PNcoarse), …

Solar Powered Conservation: The Effects Of Solar Photovoltaic Facilities On Avian Community Composition, Michael Christopher Ferrara

Graduate Theses and Dissertations

The expansion of solar photovoltaic energy infrastructure is transforming landscapes worldwide. Increasingly, facilities are planted with native vegetation, yet how vegetation characteristics and landscape variables shape wildlife communities in solar facilities remains poorly understood. Because avian populations have been in decline and are sensitive to habitat changes, understanding how solar facilities influence avian communities is important. In Chapter 1, we used autonomous recording units, local vegetation measurements, and land use and land cover data to quantify how avian community occupancy is influenced by solar cover in solar facilities and comparable reference sites. We used a Bayesian multi-species occupancy model to …

Triply Periodic Minimal Surface Design And Resin Engineering For Hybrid Piezoelectric Ceramic Composites, Joshua Zahn Renaldo Dantzler

Open Access Theses & Dissertations

Chapter I:This chapter investigates Barium Titanate (BTO)-epoxy composites reinforced with 3D-printed zirconia triply periodic minimal surface (TPMS) scaffolds. Gyroid and Primitive lattices fabricated by digital light processing (DLP) were compared with a bulk composite. The Primitive architecture showed the greatest improvement, with an elastic modulus ~ 20% higher than the Gyroid and ~ 5% higher than the bulk. Normalized piezoelectric coefficients increased substantially, with d33 enhanced by ~ 2750% and g33 enhanced by ~ 120% relative to the bulk. Under cyclic loading, the Primitive lattice generated specific voltages ~ 575% greater than the bulk and ~ 60% greater than the …

Calibration Of Low-Cost Pm2.5 Sensors Using Data Continuously Generated At A Reference Station In El Paso, Texas, Brenda Lizbeth Hernandez

Open Access Theses & Dissertations

Low-cost sensors (LCS) offer a cost-effective way to expand air-quality monitoring networks, but they require strong calibration methods to ensure reliable performance under changing environmental conditions. In this study, Clarity LCSs were co-located with a state reference monitor for 6 months to evaluate fixed-time (2-, 3-, 4-, and 6-week) and rolling (2- and 3-week) calibration approaches. A multivariate regression model using PM1, PM2.5, PM10, and relative humidity was used to generate calibration coefficients for a “base sensor.” Data from two additional sensors were normalized to the base unit using linear regression, and their calibration coefficients were applied across the network. …

Investigating The Performance Of Fibre Reinforced Plastic Repairs To Timber Marine Civil Structures At His Majesty’S Naval Base Devonport, Anna G. Walther

The Plymouth Student Scientist

This paper explores Fibre Reinforced Plastics (FRPs) as a composite repair method to restore and enhance mechanical properties of timber marine civil structures within the context of His Majesty’s Naval Base (HMNB) Devonport. To support this, timber composite specimens were manufactured and subjected to flexural and compressive strength tests. Comparisons are made to literature sources for initial predictions, in addition to hand calculations and Finite Element Analysis (FEA) models on SolidWorks. Data demonstrates that FRPs can be used as a cost-effective and beneficial method of repairing timber marine structures. Experimental data demonstrates an ability to restore compressive strength parallel to …

Development And Testing Of A Prototype Erbium-Doped Lithium Tantalate Based Sensor For Infrastructure Crack Detection And Measurement, Alejandro Barrera

Theses and Dissertations

Ensuring the safety and longevity of infrastructure is crucial to safeguarding communities and preserving economic stability. In this study, the development of a novel sensor for detecting and characterizing cracks in infrastructure, particularly suited for deployment on Unmanned Aerial Vehicles (UAVs), is presented. The device utilizes a laser (operating at 980 nm) for excitation of erbium-doped lithium tantalate nanoparticles that emit 1550 nm wavelength, which stimulates a photodiode for data collection. The data was utilized to collect, process, and reconstruct topological information. The synthesized lithium tantalate nanoparticle samples vary in erbium doping percentages of 0.5%, 3%, and 10%. Experimental evaluation …

Volume Status Detection With Integral Pulse Frequency Modulation Model Of Peripheral Venous Pressure, Jeremiah Rhys Wimer

Graduate Theses and Dissertations

This thesis studies the effectiveness of utilizing a modified integral pulse frequency modulation (IPFM) algorithm to synthesize peripheral venous pressure (PVP) waveforms for the detection of several physiological conditions using logistic regression. PVP waveforms are collected from 18 human patients and 4 porcine subjects. Human data are used to train models for determining the hypovolemic status of the patient, while the porcine data are used to train models for determining level of anesthesia and detecting internal hemorrhaging. In the human dataset, the waveforms collected from the 18 patients are classified into two groups according to serum chloride levels: resuscitated (≥100 …

Complementary Color Laser Illumination For Perceptual Contrast Enhancement In Structurally Colored Samples, Tomoshree Dash

All Theses

Structural color, a phenomenon arising from nanoscale interaction between light and materials, has the potential to unveil biological, chemical, physical, and mechanical characteristics of the sample. It enables direct visualization of the sample by human users with high spatiotemporal resolution. However, its impact is often constrained by challenges in the perceptual differentiation of subtle color variations. This thesis introduces complementary color laser illumination (C²LI) as an imaging technique that enhances color perception of the Human Visual System (HVS) by accessing the psychophysical non-linearities in chromatic color perception. C²LI offers a platform optimized for the HVS by …

Diabetes-Induced Mitral Valve Degeneration: Tissue-Engineered Solutions And Antioxidant Therapy, Samantha Hahn

All Theses

Mitral valve (MV) fibrosis is a progressive pathological condition characterized by leaflet thickening and stiffening, ultimately compromising valve function. This process is accelerated in diabetes, where chronic hyperglycemia elevates reactive oxygen species (ROS) and alters the mechanics of the extracellular matrix (ECM), collectively promoting the activation of valvular interstitial cells (VICs). Under healthy conditions, VICs maintain a quiescent, fibroblast-like phenotype responsible for sustaining ECM homeostasis. However, pathological biochemical cues or mechanical stress drive VICs toward a myofibroblast phenotype. While this transformation is essential for short-term repair, its persistence contributes to maladaptive MV remodeling and fibrosis. Diabetic stressors may be mitigated …

Mitral Annular Calcification In Diabetes: Valvular Interstitial Cells, Antioxidant Therapy, And Tissue Engineering Approaches, Raleigh J. Keenan

All Theses

Mitral annular calcification (MAC) is a chronic, progressive, and degenerative condition that impairs mitral valve function and increases the risk of cardiovascular disease. The mitral annulus is a saddle-shaped, fibrous structure that is primarily composed of collagen and elastin. The annulus also contains interspersed lipids that are involved in cellular metabolism, as well as inflammatory and osteogenic signaling. These lipids increase the annulus’ susceptibility to calcification, under conditions that increase mechanical stress (hypertension) and promote atherosclerosis (diabetes mellitus). These conditions intensify calcification by promoting the activation of valvular interstitial cells (VICs), increasing fibrosis, and inducing osteogenic differentiation. Although it is …

Experimental And Numerical Study On The Influence Of Carbon Fiber Reinforcement On The In-Plane Compressive Properties Of 3d Printed Hexagonal Honeycomb Core Structures, Deon Wallace

All Theses

There is difficulty with numerically replicating the behavior of composite honeycomb core structures numerically due to the trouble of gathering material properties from a hollow structure. Also, complex part geometries and carbon fiber reinforcement strategies can lead to unwanted structural weaknesses and variance in material properties that may not be reflected in numerical analysis when the part is additively manufactured. In this study, edgewise compressive testing is used to characterize the mechanical behavior and performance characteristics of these structures. The experimental results indicate an increase in the Young’s moduli of the honeycomb core structures as carbon fiber concentration increases. However, …

The Future Is Now: Empowering Society Through Ai Literacy, Jason S. Wrench, Sanae Elmoudden

Milne Open Textbooks

Artificial Intelligence (AI) is no longer a futuristic concept—it is the reality of the present. From the algorithms shaping our social media feeds to the generative tools transforming our workplaces, AI has permeated every aspect of modern life. The Future is Now moves beyond the hype to provide a comprehensive roadmap for understanding, navigating, and shaping this technological revolution.

Demystifying the Machine

This textbook serves as a user-friendly guide to the “black box” of AI. It breaks down complex technical concepts—from machine learning and neural networks to large language models—making them accessible to students across all disciplines. By establishing a …

Molecular Dynamics Simulations Of Biologically Relevant Interfaces: From Tear Film Lipid Layers To Rna Adsorption On Silica Surfaces., Mahbuba Khanom

Electronic Theses and Dissertations

Molecular dynamics (MD) simulation is an important tool in biochemical sciences for studying complex molecular processes and interfacial interactions at the molecular level. Biological interfaces play key roles in processes such as ocular lubrication and biomolecular adsorption. This dissertation studies interfacial phenomena involving two distinct yet conceptually related systems: the tear film lipid layer (TFLL) at the air–water interface at the surface of the human eye, and RNA adsorption on silica surfaces. These studies provide insight into how interfacial composition, structure, and environmental conditions affect larger-scale behavior. Atomistic MD simulations were used to model the TFLL. The TFLL is essential …

Engineering Risk And Indigenous Resilience Toward Intelligent Decision Support For Mining In Ancestral Lands, Izech Brian O. Edwin, King Harold A. Recto

Electronics, Computer, and Communications Engineering Faculty Publications

Mining within ancestral territories reveals an ongoing conflict between technical viability and Indigenous consent. This collaborative study, conducted by an engineering scholar and a policy researcher of Indigenous heritage in Benguet among the Ibaloi and Kankanaey peoples, investigates how risk transparency and policy alignment can bridge existing divides, while formulating principles applicable to other Indigenous mining contexts. The study provides an initial evaluation of the feasibility of a proposed large-scale mining operation within a risk-informed, data-driven governance framework. Three elements of engineering risk were assessed utilizing literature-derived and proxy parameters: (1) Land degradation is indicated by a Soil Productivity Index …

Effective Transfer Of Tumor Annotations From Hematoxylin And Eosin To Fluorescence Images Of Breast And Lung Tissues, Tianling Niu, Emi Ampo, Julie M. Jorns, Mollie Patton, Tongtong Lu, Dong Hye Ye, Tina Yen, Bing Yu

Biomedical Engineering Faculty Research and Publications

Significance: Accurate transfer of annotations from histological images to fluorescence images is essential in developing deep learning (DL)-based optical imaging systems for intraoperative assessment of tumor margins. Manual annotation is time-consuming, prone to interobserver variability, and impractical for large-scale datasets.

Aim: We present a semi-automated method that can effectively transfer tumor annotations from pathologist-annotated hematoxylin and eosin (H&E) images to fluorescence images captured using microscopy with ultraviolet surface excitation (MUSE). This method is not intended for intraoperative use but rather to facilitate the creation of annotated datasets for DL model development.

Approach: Our semi-automated method consists of nonrigid image registration, …

Synthesis And Characterization Of Chemically Recyclable Polypropylene-Based Covalent Adaptable Networks, Andrii Tiiara

All Dissertations

Polyolefins (POs) are inexpensive engineering materials with excellent physical and mechanical properties, accounting for nearly 60% of all thermoplastics. However, large-scale recycling remains limited, with only ~15% undergoing mechanical recovery. This dissertation addresses this challenge by fragmenting and functionalizing polypropylene (PP) chains to generate macromonomers and employing them, along with industrial reactive PP macromonomers, to synthesize, depolymerize, and repolymerize chemically recyclable polypropylene-based polyolefins (CR-POs). These CR-POs incorporate ester linkages that form covalent adaptable networks (CANs), maintaining a gel fraction of ~70% while remaining melt-reprocessable via extrusion and compression molding.

First, the fragmentation of PP is investigated by microwave irradiation. Microwave …

Purifying Circular Rna Therapeutics, Karen Guillen-Cuevas

All Dissertations

Circular RNA (circRNA) has emerged as a promising therapeutic modality due to its enhanced stability and resistance to exonuclease degradation compared with linear mRNA. However, its stability and functionality depend strongly on achieving high purity, and current methods such as size-exclusion chromatography suffer from peak overlap, low yields, and poor scalability. This dissertation addresses these limitations by developing ultrafiltration-based strategies to purify circRNA directly from in vitro transcription (IVT) and self-splicing (circularization) reactions. First, the feasibility of ultrafiltration was demonstrated using polyethersulfone membranes, where sieving coefficients and critical flux values of circRNA, precursor RNA, and nicked RNA were measured to …

Surface Reaction And Transportation Of Radium-226 In Marine Systems, Jiusi Wang

All Dissertations

Historical uses of radium (Ra)-containing self-luminous paint, such as on gauges and dials in naval ships, have led to the potential for such paint, and thus Ra, to be released into the marine environment. Radium-226 (²²⁶Ra) behavior in marine systems is not fully described in the literature, presenting a knowledge gap in the process of evaluating potential ecological impact of Ra in marine systems. This study explores various mechanisms contributing to Ra environmental transport in marine systems on a laboratory scale, including leaching, sorption, and diffusion. ²²⁶Ra leaching from watch hand dials containing radioactive luminous paint is …

Unraveling Nucleation Mechanisms In Membrane Distillation: A Molecular Dynamics And Enhanced Sampling Approach, Nazanin Abbasi

Graduate Theses and Dissertations

Membrane distillation offers a promising route for desalination but remains limited by salt scaling, where mineral crystals nucleate and grow in concentrated brines. To inform strategies that mitigate scaling, this thesis investigates homogeneous nucleation in supersaturated aqueous NaCl solutions using molecular dynamics and enhanced sampling techniques. Several collective variables (CVs) are tested to capture the early stages of ion aggregation and crystal formation, including ion–ion coordination number, dehydration, and bond orientational order parameters Q₄ and Q₆. Enhanced sampling techniques, such as metadynamics and umbrella sampling, are employed to overcome the high free energy barriers associated with the formation of critical …

Strategic Enhancement Of Biofuel Production Using Bacillus Subtilis As A Model Organism: A Dual Approach Of Gene Essentiality Mapping And Optimization, Angela A. Zebede

Electronic Theses and Dissertations

With rising global demand for renewable energy, enhancing microbial platforms for biofuel production is vital. This study engineered Bacillus subtilis to improve resilience to solvent stress by targeting membrane lipid biosynthesis pathways essential for integrity. Growth curve analysis (OD₆₀₀) showed that phosphatidylserine (PS) and phosphatidylethanolamine (PE) knockout strains grew significantly slower than wild type (168), indicating the importance of these lipids under stress. Laurdan fluorescence spectroscopy was used to assess membrane properties. Generalized polarization (GP) analysis revealed a concentration-dependent reduction in membrane order upon 1%–2% 1-butanol exposure. GP values declined progressively from control to 2% treatment, reflecting increased membrane fluidity …

Establishing Procedures For Primary Human Myoblast Cell Culture, Erica Duffy

Biomedical Engineering

Peripheral Artery Disease (PAD) affects an estimated 200 million people worldwide and is the leading cause of amputation in the United States. PAD is characterized by atherosclerotic narrowing of arteries, resulting in ischemia from reduced blood flow to distal tissues. Although surgical and percutaneous interventions can restore perfusion, fewer than 50% of patients experience sustained benefit, highlighting the need for alternative therapeutic strategies. One promising approach is promoting arteriogenesis, the growth and outward remodeling of pre-existing collateral vessels that improve blood flow around the occluded arteries. Arteriogenesis is regulated by regenerative, M2-polarized macrophages, making macrophage polarization a key therapeutic target. …

Component Model Development Of Heat Exchangers, Expanders, And Control Valves For Autonomous Cryogenics Plant Cool-Down, William Harris Buhrig Iv

Mechanical & Aerospace Engineering Theses & Dissertations

The traditional method of cryogenic plant cool-down involves having continuous on-call staff to head into the office at any time to modify the existing multi-layered PID control systems if the on-call staff member detects a significant deviation from the cool-down plan. This thesis aims to outline an effective method for modeling the structure of systems with performance characteristics that deviate from design requirements and from ideal inlet-outlet correspondence, enabling the adjustment and modification of existing control structures across all Thomas Jefferson National Accelerator Facility (JLab) cryogenic refrigeration plants. Analytical Modeling and Gaussian Process Regression (GPR) are applied to model the …

Per- And Poly-Fluoroalkyl Substances Fate Across California Wastewater Treatment Plants: A Meta-Analysis Of Formation, Distribution, And Persistence, Elizabeth Reineke

Master's Theses

Per- and poly-fluoroalkyl substances (PFAS) are persistent contaminants that move through municipal and industrial wastewater systems with limited attenuation. As a result, they are increasingly detected in drinking water supplies. Despite growing regulatory concern, a systems-level understanding of PFAS behavior across multiple treatment facilities and watershed settings remains incomplete. This study compiled influent, effluent, and biosolids datasets from more than one hundred California wastewater treatment plants to evaluate PFAS occurrence, transformation, and partitioning across diverse treatment configurations. Analytical steps included calculating removal efficiencies, assessing precursor-to-product oxidation, evaluating co-contaminant correlations, and grouping facilities by process design to quantify configuration-specific fate patterns. …