Digital Commons Network^™

Effects Of Light Quality And Light Quantity On The Growth Kinetics Of A Louisiana Native Microalgal/Cyanobacterial Co-Culture, Jonathan Zell Barnett

LSU Master's Theses

One of the most important factors controlling the growth of microalgae is light. Light quality and light quantity have a dramatic effect on the production of biomass, lipids and pigments of microalgae. In this research, a native Louisiana co-culture containing Chlorella vulgaris (microalgae) and Leptolyngbya sp (cyanobacteria) was studied to determine the effects of various wavelength distributions and irradiance levels on growth kinetics. Growth rates, biomass levels, lipid contents and chlorophyll-a production was examined in batch cultures to determine which color light was optimum for cultivation. At 80 µmol/m^2-sec the species shifted from microalgae dominant to cyanobacteria dominant. The impact …

Dynamic Ventilation And Power Output Of Urban Bicyclists, Alexander Y. Bigazzi, Miguel A. Figliozzi

Civil and Environmental Engineering Faculty Publications and Presentations

Bicyclist intake of air pollutants is linked to physical exertion levels, ventilation rates, and exposure concentrations. Whereas exposure concentrations have been widely studied in transportation environments, there is relatively scant research linking on-road ventilation with travel conditions and exertion levels. This paper investigates relationships among power output, heart rate, and ventilation rate for urban bicyclists. Heart rate and ventilation rate were measured on-road and combined with power output estimates from a bicycle power model. Dynamic ventilation rates increased by 0.4-0.8% per watt of power output, with a mean lag of 0.8 minutes. The use of physiology (ventilation) monitoring straps and …

Antioxidant Activity Of Lutein Entrapped In Poly (Dl-Lactide Co-Glycolide) Acid And Plga/Chitosan Nanoparticles, Toni Borel Lousteau

LSU Master's Theses

Polymeric nanocarriers improve cellular uptake, stability, solubility, and functionality of entrapped drugs and nutraceuticals. The hypothesis of this study was that entrapping lutein, a hydrophobic antioxidant, in different polymeric nanoparticles (NPs) will improve its stability and antioxidant activity. The following objectives were proposed: 1. Synthesize and characterize polymeric nanoparticles of poly lactic co-glycolic acid (PLGA) and PLGA NPs covered with a layer of chitosan (PLGA/Chi) from a physicochemical perspective, and 2. Assess functionality of the entrapped lutein as a function of type of polymer in which entrapped. Nanoparticles were synthesized by emulsion evaporation method. Characterization included size, zeta potential, and …

Impact Of Algae On Sugar Mill Effluents: Water Treatment And Added Value Assessment, Jacob Stephen Foy

LSU Master's Theses

Installations similar to Louisiana sugar mills have been proposed as potential biorefineries for sugarcane, energy cane, and sweet sorghum. The nutrient-rich wastewater generated from the milling process can be used as an algal growth medium. Simultaneously, algae can serve as a water treatment strategy to deplete these effluents of excess nitrogen, phosphorus, and organic carbon. In this research the effluents of a sugar mill were characterized for water quality and nutrient characteristics; the effluents were determined to be suitable for growth of a native Louisiana co-culture of microalgae and cyanobacteria (Chlorella vulgaris/Leptolyngbya sp.); growth rate, biomass productivity and nutrient uptake …

Developing Co-Current And Counter-Current Spray Drying Computational Fluid Dynamics (Cfd) Simulation Studies To Predict The Quality Of Microencapsulated Fish Oil With Egg White Hydrolysates Powders, Kevin Estuardo Mis Solval

LSU Doctoral Dissertations

Eggs are an excellent source of high quality protein containing all essential amino acids (EAA) including leucine. Food-grade protein hydrolysates can enhance the growth of lactic acid bacteria (LAB). Thermal degradation of bioactives normally occurs during microencapsulation by spray drying. Spray drying modeling using computational tools is essential to engineer new food powders and to minimize and thermal degradation of bioactives. The first chapter of this work discusses the optimization of an enzymatic process in terms of reaction temperature, pH, enzyme:substrate ratio, and reaction time using a response surface methodology (RSM) to produce food-grade protein hydrolysates from egg whites (EWH). …

Ecological Sanitation In Uganda: Promotion Through Demonstration Facilities And Potential For Ascaris Reduction By Free Ammonia Inactivation Using Stored Urine, John Thomas Trimmer

USF Tampa Graduate Theses and Dissertations

As Uganda works to transform itself into an industrialized, middle-income country in the coming decades, the country is faced with a number of problematic trends that could hinder this transition. High population growth and urbanization are quickly forcing small towns to deal with issues of limited space and the aesthetic conditions within sanitation systems, while declining soil fertility in surrounding rural areas calls into question the future nutritional security of the growing population. Ecological Sanitation (Eco-San) systems, which are designed to recover nutrients from human excreta, may help to address these trends.

Improved sanitation coverage in Uganda is currently estimated …

Assessment Of Quality Stability Of Composite Flours Produced By Dar Es Salaam Smes, Leonard Rweyemamu

Tanzania Journal of Engineering and Technology (TJET)

A study was conducted to assess quality stability of cereal flours processed by food processing SMEs in Dar es Salaam. This product is obtained by mixing various cereal grains and is considered as of higher nutritional values in Tanzania. The shelf-life was predicted by determining water activity (aw) and moisture content (MC) levels and plotting the water sorption isotherms (MSI). Ninety per cent of investigated samples (N=78) were found packaged in the materials of various quality conditions. It was found that the packaging materials mostly used is plastic films (63%), paper packets/bags (26%), hard papers (3%), plastic jars (1%) and …

Analysis Of Heart Rate Variability Amongst Cyclists Under Perceived Variations Of Risk Exposure, Ronan Doorley, Vikram Pakrashi, Eoin Byrne, Samuel Comerford, Bidisha Ghosh, John A. Groeger

NIMBUS Articles

Cycling as a mode of travel provides an opportunity for many people to increase their levels of regular physical activity and contribute to their mental and physical health. Heart rate is often used as a means of measuring the intensity and energy expenditure of physical activity. However, heart rate is also linked to emotional factors such as anxiety and fear. Perceptions of risk due to external factors such as other road users and infrastructure may arouse such emotions in urban cyclists. The present study set out to investigate whether or not perceptions of risk among urban cyclists may lead to …

Metabolic Engineering Of Cyanobacteria For Photosynthetic Production Of Drop-In Liquid Fuels, Bertram Michael Berla

McKelvey School of Engineering Graduate Student Theses & Dissertations

Cyanobacteria are oxygenic phototrophs with great potential as hosts for renewable fuel and chemical production. They grow very quickly (compared with plants) and can use sunlight for energy and CO2 as a carbon source (unlike yeast or E. coli). While cyanobacteria have been engineered to make many chemicals that are native and non-native parts of their metabolism, this work is concerned with the production of heptadecane in Synechocystis sp. PCC 6803. Heptadecane is in a class of natural products produced by all cyanobacteria, but in quantities insufficient for industrialization. Towards this future goal, we have built enabling systems for the …

Differential Protein Expression Of Caco-2 Cells Treated With Selenium Nanoparticles Compared With Sodium Selenite And Selenomethionine, Linglin Fu, Xuxia Yan, Xinming Ruan, Junda Lin, Yanbo Wang

Biomedical Engineering and Sciences Faculty Publications

The study was designed to determine the differential protein expression of Caco-2 cells treated with different forms of selenium including sodium selenite, selenomethionine (Se-Met), and selenium nanoparticles (nano-Se). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS) were used to identify the differentially expressed proteins. The results indicated that seven protein spots, ubiquitin-conjugating enzyme E2 (E2), glutathione synthetases (GS), triosephosphate isomerase (TSP), T-complex protein 1 subunit zeta (TCPZ), lamin-B1, heterogeneous nuclear ribonucleoprotein F (hnRNP F), and superoxide dismutase [Cu-Zn] (Cu, Zn-SOD) were significantly different among all the groups. According to the order of control, sodium selenite, Se-Met, and Nano-Se, the …

Developing Critical Loads And Dynamic Critical Loads For Acidification For Watersheds In The Adirondack Region Of New York And Great Smoky Mountain National Park (Grsm), Qingtao Zhou

Dissertations - ALL

Critical loads (CLs) and dynamic critical loads (DCLs) are important tools for the management of ecosystems that are impacted by high sulfate, nitrate and ammonium deposition. In this study, a biogeochemical model (PnET-BGC) was applied to 20 watersheds in the Adirondack region and 12 watersheds in Great Smoky Mountain National Park (GRSM) to calculate CLs and DCLs. I evaluated ecosystem changes in response to historical and potential future changes in acidic deposition. I analyzed factors affecting CLs and DCLs for acidification in the acid impacted Constable Pond Watershed in the Adirondack region, specifically evaluating trade-offs of sulfate and nitrate deposition, …

Feedlot Manure Management Considerations Including Anaerobic Digestion Potential And Mineral Retention, Andrea K. Watson

Department of Animal Science: Dissertations, Theses, and Student Research

Three anaerobic digestion trials were conducted to evaluate methane production from feedlot manure. As organic matter content of manure decreased, degradation of manure and methane production also linearly decreased (P ≤ 0.02). Quality, or organic matter content of manure, had a greater impact on anaerobic digestion than diet (with and without distillers grains) fed to cattle. Minerals are concentrated in effluent removed from anaerobic digesters.

Knowing mineral retention within cattle allows for more accurate calculation of mineral excretion in manure. Three serial slaughter trials measured mineral retention in growing and finishing cattle. During a growing phase, beef cattle gaining …

Microanalysis Of Polymer Chain Diffusion In Heat Seals, Russell Cooper

All Theses

Heat sealing is an integral method for the closure and protection of packaging. Previous work has shown that seal strength is developed by the interdiffusion of polymer chains within heat seals. Heat seals were made between two dissimilar materials. Poly(ethylene-co-acrylic acid) (EAA) was heat sealed to ionomer. Diffusion within the EAA-ionomer heat seals was estimated. The diffusion estimates were then related to resulting seal strength in the EAA-ionomer sealant system. Heated tooling sealing was utilized to make heat seals at 40 psi (275.79 kPa), 0.5 seconds, and a range of temperatures between 180˚F (82.22˚C) and 300˚F (148.89˚C). Scanning electron microscopy …

The Effects Of Chlorine Dioxide Gas On The Sensory Properties Of Whole Tomatoes, Christopher Gottilla

All Theses

This experiment sought to measure the changes in sensory quality that occur in tomatoes as the result of chlorine dioxide gas treatment. Tasti-Lee tomatoes (VFFF Hybrid #5755) were treated with two different levels of chlorine dioxide gas (10mg and 50mg of ClO2-forming material per kg of tomato) utilizing sachets. Testing compared the treated tomatoes and an untreated control group. These tests included flat-plate compression for firmness, sensory evaluation using a difference from control test, color analysis of the skin and flesh using a colorimeter, and enumeration of aerobic microbes and yeasts and molds. Flat-plate compression of whole tomatoes revealed no …

Photobioreactor Design For Improved Energy Efficiency Of Microalgae Production, Alexander Burns

Master's Theses

ABSTRACT

Photobioreactor Design for Improved Energy Efficiency of Microalgae Production

Alexander Burns

The objective of this research was to investigate a new photobioreactor (PBR) design for microalgae production that retains the typical advantages of existing tubular PBRs while reducing power consumption by providing simultaneous culture circulation and gas exchange with airlift alone and no centrifugal recirculating pump. Traditional tubular PBR designs feature a compressed air supply and a centrifugal pump for culture circulation and gas exchange. Circulation and gas exchange in a closed-system PBR is necessary to keep the algae suspended and to provide sufficient mass transfer (mainly for the …

Relationship Between Peel Force, Opening Force, And Burst Force For A Semi-Rigid Cup And Lid, Pre- And Post-Retort, Raj Navalakha

All Theses

This research determines if there is any relationship between the peel force, the opening force and the burst force for a semi-rigid cup and lid system, for pre- and post-retort conditions. It also compares the relationship (regression lines) between these forces pre- and post-retort. These seal results were studied by varying the sealing parameters of dwell time and temperature while keeping the pressure constant. Polypropylene cups and a peelable barrier retort lidding were used in this study. As compared to past research, a different peel testing technique was used to measure the peel and the opening force. The entire lid …

Research: South Dakota State University, Winter 2014, Christie Delfanian, Eric Landwehr

State of Discovery South Dakota State University's Research Magazine

CONTENTS:

Weeds influence gene expression, growth in corn [Page] 2
Graduate research targets childhood obesity [Page] 3
Transmammary drug delivery system for early-stage breast cancer may reduce side effects [Page] 5
Biochemical pathways may be key to scab resistance [Page] 7
New filtration system saves water, money for City of Sioux Falls [Page] 8
Doulas ease stress, increase satisfaction with the birthing experience [Page] 9
Structure lab evaluates strength, durability of structural components [Page] 10
Device gives sunflower producers reprieve from combine fires [Page] 12
Graduate scholarship winner works on renewable energy storage [Page] 12

Wildlife Deterrence Method Test Device, Garrett Tietz, Adam Webb, Dane Knutson

Mechanical Engineering

The objective of the Deer Busters team is to design and build a device or system of devices that will be used to determine which method, or methods, are most effective at deer deterrence. JumpSport suspects that a method which gives the appearance of approach to the deer in an aggressive or startling manner but also changes the way it attacks so that the deer do not get used to the device will be most effective. Deer Busters is committed to the completion of the deer deterrent testing device by the end of the fall quarter of 2014.

Long-Acting Antituberculous Therapeutic Nanoparticles Target Macrophage Endosomes, Benson J. Edagwa, Dongwei Guo, Pavan Puligujja, Han Chen, Joellyn Mcmillan, Xinming Liu, Howard Gendelman, Prabagaran Narayanasamy

Nebraska Center for Biotechnology: Faculty and Staff Publications

Eradication of Mycobacterium tuberculosis (MTB) infection requires daily administration of combinations of rifampin (RIF), isoniazid [isonicotinylhydrazine (INH)], pyrazinamide, and ethambutol, among other drug therapies. To facilitate and optimize MTB therapeutic selections, a mononuclear phagocyte (MP; monocyte, macrophage, and dendritic cell)-targeted drug delivery strategy was developed. Long-acting nanoformulations of RIF and an INH derivative, pentenyl- INH (INHP), were prepared, and their physicochemical properties were evaluated. This included the evaluation of MP particle uptake and retention, cell viability, and antimicrobial efficacy. Drug levels reached 6 μg/106 cells in human monocyte-derived macrophages (MDMs) for nanoparticle treatments compared with 0.1 μg/106 cells for native …

Fresh Bytes - Connected Hydroponics For Small-Scale Growing, Jack Bowen

Liberal Arts and Engineering Studies

Many users are now transitioning to small-scale hydroponics and aquaponics at home. There can be a barrier to entry with these systems as there is a delicate balance of chemicals, pH, etc. that must be maintained. There are sensors for these various components but they are either aimed at commercial production or are un-automated. Fresh Bytes is a microcomputer with sensors to detect all of these unseen components in a hydroponic system. A prototype of this microcomputer is produced along with CAD plans for more professional versions of it. The sensors are verified and future development is contemplated.

Effect Of Different Extrusion Processing Parameters On Physical Properties Of Soy White Flakes And High Protein Distillers Dried Grains-Based Extruded Aquafeeds, Sushil Kumar Singh, Kasiviswanathan Muthukumarappan

Agricultural and Biosystems Engineering Publications

Nutritionally balanced ingredient blends for catla (Catla catla), belonging to the family Cyprinidae, were extruded using single screw extruder. The extrusion was carried out at five levels of soy white flakes content (21%, 29%, 40%, 52%, and 59% db), five levels of moisture content (15, 19, 25, 31, and 35% db) and five levels of barrel temperature (100, 110, 125, 140, and 150 ºC) using three different die nozzles (having L/D ratios 3.33, 5.83, and 7.25). Blends with net protein content of 32.5% contains soy white flakes, along with high protein distillers dried grains (HP-DDG), corn flour, corn gluten meal, …

Combining Modified Atmosphere Packaging And Nisin To Extend The Shelf Life Of Atlantic Salmon (Salmo Salar), Dong Han

All Theses

Atlantic salmon is often used in laboratory experiments to represent marine fishes. Many researchers try to optimize the levels and dosage of preservatives to maintain the freshness and extend the shelf-life of Atlantic salmon. Little information has been provided about the effectiveness of combined method between MAP and nisin, especially on the preservation of Atlantic salmon. Strong evidences suggest that MAP associated with nisin may have a potential to improve Atlantic salmon storage quality. Preservation effectiveness of combining modified atmosphere package (MAP) and nisin on fresh Atlantic salmon was determined using various methods. Six groups of farmed Atlantic salmon were …

The Development And Characterization Of Tannic Acid Cross-Linked Collagen Scaffolds For The Prevention Of Breast Cancer Recurrence In Lumpectomy Patients, Beau Inskeep

All Dissertations

Breast cancer is the second most common cancer amongst women, second only to lung cancer. In 2014, it was estimated that there were approximately 295,000 new cases of breast cancer diagnosed in women, with approximately 40,000 deaths caused by breast cancer in the United States. It has been reported that a woman has a 12% chance of developing breast cancer in her lifetime and a 3% chance of dying from breast cancer. There are several treatment options available for breast cancer, including chemotherapy, radiation therapy, as well as two different forms of surgery, mastectomy and lumpectomy. However, none of these …

Solar Brine Concentrator, Aric Gregory Fisher

Chemical Engineering Undergraduate Honors Theses

In arid regions of the United States potable water is a valuable resource and is sometimes produced using energy intensive processes by removing salts from brackish water. Task 4 provides a solar option for producing potable water from brackish water; in competing in Task 4, H2OGS has developed a simple, cost effective solar desalination process to provide these regions with necessary, potable water. H2OGS’ design takes into account WERC requirements, including cost, energy usage, worker safety, and easily obtained materials. U.S. government regulatory requirements are also taken into account as the proposed location for this process is Alamogordo, New Mexico. …

Werc: Solar Brine Concentrator, Sarah K. Haley

Chemical Engineering Undergraduate Honors Theses

In arid regions of the United States potable water is a valuable resource and is sometimes produced using energy intensive processes by removing salts from brackish water. Task 4 provides a solar option for producing potable water from brackish water; in competing in Task 4, H2OGS has developed a simple, cost effective solar desalination process to provide these regions with necessary, potable water. H2OGS’ design takes into account WERC requirements, including cost, energy usage, worker safety, and easily obtained materials. U.S. government regulatory requirements are also taken into account as the proposed location for this process is Alamogordo, New Mexico. …

Protective Effects Of Milk Phospholipids Against Uv-Induced Dna Damage In Human Skin Cells, Lan-Anh Nguyen

Master's Theses

Skin cancer is the most common type of cancer in the US. The American Academy of Dermatology estimated that more than 3.5 million new cases of skin cancer are diagnosed in the US each year and 1 in 5 Americans will likely to develop skin cancer in their life time. Most cases of skin cancer are caused by exposure to ultraviolet (UV) radiation from the sun. Some of the most common sunscreen ingredients are unstable and can form harmful radicals upon exposure to UV radiation. There is a strong clinical need for a more stable and effective sunscreen ingredient such …

Targeted Drug Delivery System For Kidney And/Or Liver Failure Patients Using Human Serum Albumin, Sean Bedingfield

Undergraduate Honors Capstone Projects

Compromised liver and/or kidney function reduces the acceptable dosage of a variety of medications that can be administered to patients. These patients still have a need for drugs such as nonsteroidal anti-inflammatory drugs (NSAIDs), antivirals, and antibiotics. The project goal is to provide a drug delivery system to accommodate these reduced dosage limits with added therapeutic benefits to address symptoms of liver or kidney failure. Localized drug delivery allows for a smaller, concentrated dose rather than inundating the patient's system with the drug of interest. Human serum albumin (HSA) is a researched candidate for drug delivery with therapeutic properties. HSA …

Effects Of Transportation Hazards On Package Performance And Food Product Shelf Life, Kyle Dunno

All Dissertations

This research studied the effect of transportation hazards on food product shelf life and package performance. Studies were conducted to determine the effect of package headspace volume, product viscosity and storage temperature on package integrity. Finally, accelerated shelf life testing (ASLT) was utilized to determine how simulated transportation hazards affected the shelf life of a specific food product. Institutional retort pouches containing either water or 5% starch solution were filled with varying amounts of headspace volume to determine if package headspace volume could aid in package performance during simulated engineering tests for packaged products. Fixed displacement vibration testing and compression …

Potential For Optical Sensor-Based Nitrogen Fertilization In Grain Sorghum (Sorghum Bicolor L. Moench) In Arkansas, Kamil H. Rosales Rodriguez

Graduate Theses and Dissertations

Ground-based active-optical (GBAO) crop sensors have become an effective tool to improve nitrogen (N) use efficiency and to predict yield early in the growing season, particularly for grass crops. Commercially available canopy sensors calculate the normalized difference vegetative index (NDVI) by emitting light in the red and near infrared range of the electromagnetic spectrum. The NDVI is used to evaluate vigor status and to estimate yield potential. However, few studies have been conducted to compare the performance of commercially available sensors. Therefore, a study was conducted using the most common crop canopy sensors: i) N-Tech's GreenSeeker^TM (GS), ii) Holland …

Development Of A Reusable Nutrient Recovery System To Sustainably Integrate Small-Scale Sanitation And Agriculture, Jorge Luis Calabria

USF Tampa Graduate Theses and Dissertations

Emerging technologies in sanitation and food production foster the potential to advance progress towards key sustainable development goals of ensuring adequate sanitation and food security to all. Non-Sewered Sanitation Systems (NSSSs), as detailed by the recent International Organization for Standardization’s (ISO) 30500 standard, are being developed to overcome challenges of lacking sanitation infrastructure and resource scarcity by providing autonomous, off-grid wastewater treatment (WWT) service in a much smaller footprints than large centralized treatment facilities. A promising NSSS platform is the anaerobic membrane bioreactor (AnMBR) that can achieve high (WWT) throughput in a small footprint using ultrafiltration (UF) membrane filtration to …