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Using A Lubricin Reporter Cell To Test Current Vs. Optimized Media Compositions, Sean M. Kennedy

Honors Undergraduate Theses

Osteoarthritis is a joint disease characterized by the breakdown of articular cartilage. The field of tissue engineering is interested in developing methods to produce biological alternatives to current orthopedic procedures. Lubricin is a molecule which is important in the proper lubrication of articular cartilage. It is a challenge in the field of tissue engineering to produce cartilage with sufficient lubricin expression. Developing a reporter cell for lubricin allowed for a more efficient investigation of the conditions wh­­­ich may influence its expression. By comparing "optimized" and traditional media solutions, it was determined that the use of a previously reported type II …

Life Cycle Assessment Of Polylactic Acid Biopolymer Industrial Waste Management Techniques In Belize, Dennis J. Newby

Theses and Dissertations--Civil Engineering

In January 2020, the Government of Belize enacted an Implementation Strategy and Action Plan to phase-out single-use plastics and to transition to products like bioplastics. This work investigated the environmental effects of using alternative waste management techniques to manage polylactic acid biopolymer (PLA) waste by using life cycle assessment (LCA). The following treatment options were compared: landfill, landfill expansion, cogeneration, and anaerobic digestion. The landfill and landfill expansion processes both had a global warming potential of 0.01 kg of CO₂ eq. per kg of PLA waste managed compared to the cogeneration and anaerobic digestion processes -0.03 and -0.06 kg …

Use Of Microscale Hydrophobic Surface Features For Integration Of 3d Cell Culture Into Multi-Functional Microfluidic Devices, Soroosh Torabi

Theses and Dissertations--Mechanical and Aerospace Engineering

3D cell culture and microfluidics both represent powerful tools for replicating critical components of the cell microenvironment; however, challenges involved in integration of the two and compatibility with standard tissue culture protocols still represent a steep barrier to widespread adoption. Here we demonstrate the use of engineered surface roughness in the form of microfluidic channels to integrate 3D cell-laden hydrogels and microfluidic fluid delivery. When a liquid hydrogel precursor solution is pipetted onto a surface containing open microfluidic channels, the solid/liquid/air interface becomes pinned at sharp edges such that the hydrogel forms the “fourth wall” of the channels upon solidification. …

Additive Manufacturing In Personalized Medicine: Patient-Customized Solid Dose Medicines, And Patient-Customized Wound Care Device, Amir Najarzadeh

Theses and Dissertations--Mechanical and Aerospace Engineering

Personalized Medicine - from a one-size fits all to a tailored approach is the future in pharmaceutical therapeutics and medical applications. The aim of this study is demonstrate additive manufacturing in two personalized medicine applications: 1. Patient- customized solid dose medicines, and 2. Patient-customized wound care device. The aim of the first application is to develop an inexpensive and accurate method to design and fabricate patient-customized tablets. Particularly this paper focuses on fabricating and dose controlling of patient-customized extended release prednisolone-poly(vinyle alcohole) PVA. Dry-Blending, Wet Granulation, Extrusion, Spherionization, Hot melt extrusion (HME) was adopted to produce drug loaded prednisolone-PVA filaments …

Assessing The Use Of Lidar And Uav Technology For Monitoring Growing Alfalfa, Stuart Tucker Sheffield

Theses and Dissertations--Biosystems and Agricultural Engineering

Alfalfa is a popularly grown crop because of its value as a nutritious feed source for livestock. The efficient production of an alfalfa crop relies on the monitoring of certain parameters, like height, quality, and yield. Traditionally, producers have used manual measurements of alfalfa plant height to estimate the nutritive quality and yield of a growing alfalfa crop. Manual measurements of plant height are often labor intensive and provide low resolution data that is not acceptable for full field scale assessment of growing alfalfa. The two studies presented in this thesis offer detailed insight into the rapid and accurate monitoring …

Modeling Energy Flows In Floating In-Pond Raceways Utilizing Solar Power Back-Up, Bo Smith

Theses and Dissertations--Biosystems and Agricultural Engineering

The In-pond Raceway (IPR) is a novel option for production aquaculture, depending on water moving devices to provide constant flow. Device failure may result in catastrophic fish loss, requiring power backup systems to mitigate risk in case of power outages. Because these systems must be dependable and many suitable locations are remote, off-grid solar photovoltaic (PV) systems with battery storage have been considered since they eliminate need for utility power. Such systems can be hard to size and expensive. This study modeled system requirements using an energy balance to determine whether systems could withstand varying loads based on climatological conditions. …

Econometric Frameworks For Energy Prediction, Naveen Chandra Iraganaboina

Electronic Theses and Dissertations, 2020-2023

Global warming and associated role of energy consumption across various sectors is a well-researched topic in recent years. Understanding current urban energy consumption patterns will allow us to understand how future energy consumption patterns will evolve. With electrification of vehicles and potentially altering culture of work from home, the energy usage at regional level would see a significant change in the future. The current PhD dissertation contributes to energy consumption analysis of a region by analyzing residential energy consumption, commercial energy consumption and transportation energy use by households. The aggregation of these energy consumption within a region contributes to the …

Open-Source Tig-Based Metal 3d-Printing, Shane Oberloier

Dissertations, Master's Theses and Master's Reports

Metal 3-D printing has been relegated to high-cost proprietary high-resolution systems and low-resolution low-cost metal inert gas (MIG) systems. In order to provide a path to high-resolution, low-cost, metal 3-D printing, this manuscript proposes a new open source metal 3-D printer design based around a low-cost tungsten inert gas (TIG) welder coupled to a commercial open source self replicating rapid prototyper. Optimal printing parameters for the machine are acquired using a novel computational intelligence software. TIG has many advantages over MIG, such as having a low heat input, clean beads, and the potential for both high-resolution prints as well as …

Ogt Glycosylation Of Histones In T Cells May Participate In Systemic Lupus Erythematosus, Jared Luke Ridgeway

Honors Theses

Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disease that primarily affects women. The etiology is not known yet, but genetics and environmental factors trigger the disease. These epigenetic changes modify gene expression and contribute to the development of the disease. It is known that T cells from patients with lupus overexpress genes that may contribute to the onset and progression of the disease. Dr. Gorelik has shown that OGT (O-linked N-acetyl-Glucosamine Transferase), which is an X-linked gene, is overexpressed in women with SLE. OGT adds b-N-acetylglucosamine (O-GlcNAc) to serine and threonine residues of proteins. …

Sensitray: An Integrated Measuring Device For Monitoring Children’S Mealtime Dietary Intake, Hunter S. Guru, Anthony D. Weng, Santosh Pitla, Dipti Dev

Department of Child, Youth, and Family Studies: Faculty Publications

Childhood nutrition establishes consumption norms that affect an individual’s health over the course of their lives. However, early nutrition interventions to establish such norms are uncommon owing to the various inefficiencies associated with current methods of measuring childhood nutrition. Here, we present an IoT measuring device, called the SensiTray, which accurately tracks mealtime intake in a child-friendly and cost-effective fashion. Principal technologies underlying the SensiTray (including mass-sensing technologies, microcontrollers) are identified and analyzed, along with other design choices. Operation of the SensiTray is explained with special attention given to SensiTray software peripherals and algorithms. Preliminary testing consisted of static and …

Low-Temperature Gas Plasma Combined With Antibiotics For The Reduction Of Methicillin-Resistant Staphylococcus Aureus Biofilm Both In Vitro And In Vivo, Li Guo, Lu Yang, Yu Qi, Gulimire Niyazi, Jianbao Zheng, Ruobing Xu, Xusong Chen, Jingye Zhang, Wang Xi, Dingxin Liu, Xiaohua Wang, Hailan Chen, Michael G. Kong

Electrical & Computer Engineering Faculty Publications

Biofilm infections in wounds seriously delay the healing process, and methicillin-resistant Staphylococcus aureus is a major cause of wound infections. In addition to inactivating micro-organisms, low-temperature gas plasma can restore the sensitivity of pathogenic microbes to antibiotics. However, the combined treatment has not been applied to infectious diseases. In this study, low-temperature gas plasma treatment promoted the effects of different antibiotics on the reduction of S. aureus biofilms in vitro. Low-temperature gas plasma combined with rifampicin also effectively reduced the S. aureus cells in biofilms in the murine wound infection model. The blood and histochemical analysis demonstrated the biosafety of …

Matters Of Biocybersecurity With Consideration To Propaganda Outlets And Biological Agents, Xavier-Lewis Palmer, Ernestine Powell, Lucas Potter, Thaddeus Eze (Ed.), Lee Speakman (Ed.), Cyril Onwubiko (Ed.)

Electrical & Computer Engineering Faculty Publications

The modern era holds vast modalities in human data utilization. Within Biocybersecurity (BCS), categories of biological information, especially medical information transmitted online, can be viewed as pathways to destabilize organizations. Therefore, analysis of how the public, along with medical providers, process such data, and the methods by which false information, particularly propaganda, can be used to upset the flow of verified information to populations of medical professionals, is important for maintenance of public health. Herein, we discuss some interplay of BCS within the scope of propaganda and considerations for navigating the field.

Multi-Dimensional Modeling For Environmental Impact Assessment At Intersections Of The Food-Energy-Water Nexus, Jessica Daignault

Dissertations, Master's Theses and Master's Reports

This dissertation uses multi-dimensional modeling for environmental impact assessment at intersections of the Food-Energy-Water (FEW) Nexus, including life cycle assessment (LCA) modeling for quantification of environmental impacts due to household FEW consumption, a linear regression framework for quantification of water-use impacts of marginal electricity generation, and a multi-objective optimization model to assess monetization of water withdrawals for electricity generation and impacts to water stress due to electricity dispatch schemes. Chapter 2 of this dissertation summarizes the development of an LCA model that quantifies the direct and indirect environmental impacts of household FEW consumption. The model is executed through a novel …

The Development Of A Wearable Device For Relieving The Lower Leg Muscles Fatigue, Warawarin Nitirojntanad

Chulalongkorn University Theses and Dissertations (Chula ETD)

Standing is considered one of the most practical working postures since our limbs have the greatest range of motion. Standing yields plenty of benefits, however, prolonged standing could lead not only to discomfort but also to musculoskeletal disorders (MSDs). This study aims to design and develop a device that could help relieve muscle fatigue in the lower leg as the effects of prolonged standing as well as to evaluate the proposed device. The basics of fatigue, the consequences of prolonged standing and walking, and a review of the existing interventions to cope with muscle fatigue are used in designing the …

Altered Skeletal Muscle Excitation-Contraction Coupling In The R6/2 Transgenic Mouse Model For Huntington's Disease, Daniel R. Miranda

Browse all Theses and Dissertations

Huntington’s disease (HD) has classically been categorized as a neurodegenerative disorder. However, the expression of the disease-causing mutated huntingtin gene in skeletal muscle may contribute to the symptoms of HD, namely those that involve involuntary muscle contraction. In the R6/2 transgenic mouse model of HD, we previously observed ion channel defects that could contribute to involuntary muscle contraction. Here, in R6/2 muscle we investigated the consequence of these ion channel defects on action potentials (APs), the first step in excitation-contraction (EC) coupling. We found that the ion channel defects were associated with depolarizing the baseline membrane potential during AP trains. …

Accelerometer-Based Vigilance State Classification In Dairy Cows, Evan King

Theses and Dissertations--Electrical and Computer Engineering

Globally, dairy farming is a $700 billion industry, with more than 9 million dairy cows in the United States alone. Depriving cows of required activities such as sleep has been shown to negatively impact reproductive efficiency, decrease the volume of milk produced, and increase the risk of culling. Overcrowded herds can decrease individual animal health, demanding the need for automatic behavior detection that would provide insight into their state of health.

Using electroencephalography (EEG), electrooculography (EOG), and electromyography (EMG) to characterize the phases of sleep is a technique which has been used for decades. While these techniques are considered the …

Editorial: Predictive Modeling Of Human Microbiota And Their Role In Health And Disease, Hyun-Seob Song, Stephen R. Lindemann, Dong-Yup Lee

Department of Agricultural and Biological Systems Engineering: Faculty Publications

No abstract provided.

Pectin Modified Metal Nanoparticles And Their Application In Property Modification Of Biosensors, Meena Nemiwal, Tian C. Zhang, Dinesh Kumar

Department of Civil and Environmental Engineering: Faculty Publications

Pectin is a structural anionic heteropolysaccharide and abundantly found in the cell wall of terrestrial trees and plants. It exhibits several advantageous properties such as non-toxicity, cheap, biodegradable, biocompatible, abundant, flexible, etc. Functional groups like carboxylic acid and hydroxyl make pectin suitable to be covalently bonded with other biomolecules and proteins. Based on these properties, pectin is being extensively employed to encapsulate/coat metal nanoparticles (MNPs) to inhibit their aggregation and enhancing the suitability of MNPs for a wide range of applications in healthcare like drug delivery, antimicrobial activity, antioxidant etc. Another important application of pectin is to enhance the electrochemical …

Transmission Routes Of The Microbiome And Resistome From Manure To Soil And Lettuce, Yuepeng Sun, Daniel D. Snow, Harkamal Walia, Xu Li

Daugherty Water for Food Global Institute: Faculty Publications

The land application of animal manure can introduce manure microbiome and resistome to croplands where food crops are grown. The objective of this study was to characterize the microbiome and resistome on and in the leaves of lettuce grown in manured soil and identify the main transmission routes of microbes and antibiotic resistance genes (ARGs) from soil to the episphere and endosphere of lettuce. Shotgun metagenomic results show that manure application significantly altered the composition of the microbiome and resistome of surface soil. SourceTracker analyses indicate that manure and original soil were the main source of the microbiome and resistome …

Osteocyte Activity And Skeletal Muscle Relative Gene Expression Profiling After Short-Term Muscle Paralysis, Michelle Gelbs

Dissertations and Theses

Mechanical loading is essential for maintaining bone tissue. Reduced mechanical loading has been shown to have a negative effect on bone, and can result in the development of disuse osteoporosis. Disuse models of muscle inactivity and immobilization, like the Botox model used in this study, result in changes in the bone microarchitecture, the mechanisms behind which are not fully understood. In a previous four-week Botox disuse study, skeletally mature 20- week-old rats experienced degradation of intracortical bone, increased vascular porosity, and decreased osteocyte lacunar density in the tibiae. The focus of this study was to explicate a potential source of …

Development Of An Injectable Methylcellulose Hydrogel System For Nucleus Pulposus Repair And Regeneration, Nada A. Haq-Siddiqi

Dissertations and Theses

Low back pain is the most common cause of disability in the world and is often caused by degeneration or injury of the intervertebral disc (IVD). The IVD is a complex, fibrocartilaginous tissue that allows for the wide range of spinal mobility. Disc degeneration is a progressive condition believed to begin in the central, gelatinous nucleus pulposus (NP) region of the tissue, for which there are few preventative therapies. Current therapeutic strategies include pain management and exercise, or surgical intervention such as spinal fusion, none of which address the underlying cause of degeneration. With an increasingly aging population, the socioeconomic …

Assessing Heart Rate Using Consumer Technology Association Standards, Joel D. Reece, Jennifer A. Bunn, Minsoo Choi, James W. Navalta

Kinesiology and Nutrition Sciences Faculty Research

It is difficult for developers, researchers, and consumers to compare results among emerging wearable technology without using a uniform set of standards. This study evaluated the accuracy of commercially available wearable technology heart rate (HR) monitors using the Consumer Technology Association (CTA) standards. Participants (N = 23) simultaneously wore a Polar chest strap (criterion measure), Jabra Elite earbuds, Scosche Rhythm 24 armband, Apple Watch 4, and Garmin Forerunner 735 XT during sitting, activities of daily living, walking, jogging, running, and cycling, totaling 57 min of monitored activity. The Apple Watch mean bias was within ±1 bpm, and mean absolute percent …

Evaluating And Predicting The Risk Of Algal Blooms In A Freshwater Lake Through A 4-Dimensional Approach: A Case Study On Lake Mitchell, Sumit Kumar Ghosh

Electronic Theses and Dissertations

Excessive algal growth in freshwater lakes can negatively impact ecosystems, recreation, and human health. Though algae are a natural part of freshwater ecosystems, elevated nutrient loading from anthropogenic and natural sources can lead to algal blooms. Both algae and blue-green algae (BGA) are responsible for algal blooms; however, BGA (cyanobacteria) is more dangerous. The first objective of this research was to prepare a conceptual model to understand how various environmental variables affect algae. This conceptual model was used to choose the environmental variables that help increase or decrease algae in the water environment. The second objective was to develop empirical …

Strategies For Catalyzing Clinicians’ Support Of Telemedicine Programs In Rural Communities, Jonathan Liwag

Walden Dissertations and Doctoral Studies

Clinicians’ failure to accept contemporary technology has been a critical barrier to telemedicine program adoption. Staff technical challenges and resistance to change can affect technology return on investment, which concerns telemedicine program leaders. Grounded in Davis’s technology acceptance model, the purpose of this qualitative, descriptive single case study was to explore strategies used by program leaders in the Marianas Islands who successfully gained clinicians’ acceptance of contemporary technology. The participants were 5 program leaders recommended by their institution because of their leadership role and telemedicine technologies experience. Data were collected from 4 face-to-face and 1 audio teleconference interview with the …

Quantifying Avoidable Food Waste And Identifying Its Underlying Causes: A Case Study Of A University Dormitory In Thailand, Shimpei Iwasaki, Suphat Prasopsin, Thamarat Phutthai

Applied Environmental Research

To develop well-planned and effective policies and programs for reducing avoidable food waste, it is important to quantify the actual food waste level in particular settings and assess relationships among consumers’ awareness, attitudes, and behaviors. Recognizing these considerations, this paper measured avoidable food waste generated by university students living in dormitory buildings and identified its underlying causes in the case of Kanchanaburi campus, Mahidol University, Thailand. The study applied a food waste composition survey 18 times between January and May 2019 while administering questionnaires in October 2019 to the dormitory students. Based on these measures, the study identified 1,417 instances …

From Bench To Worktop: Rapid Evaluation Of Nutritional Parameters In Liquid Foodstuffs By Ir Spectroscopy, David Perezguaita

Articles

We evaluated the use of attenuated total reflectance infrared spectroscopy for simultaneous in situ quantification of the nutritional composition of liquid food stuffs in the industrial kitchen context. Different methodologies were compared, including dry and wet acquisition along with instrument parameters and measurement times of 4 and 60 s. The most effective technique was 1-minute measurement, with prediction errors of 2.6, 0.7, 1.0, 2.2, 0.8, 2.4 g/100 mL and 150 Kcal, for carbohydrates, proteins, fat, sugars, saturated fat, water and energy values, respectively.The 4-second method resulted in larger errors but was more applicable for inline measurements. Dry measurements successfully predicted …

Removal Of Ferrous And Arsenic From Contaminated Groundwater By Co-Precipitation Coupling With Membrane Separation Process, Bonita Pen

Chulalongkorn University Theses and Dissertations (Chula ETD)

This work aimed to​ treat contaminated groundwater with arsenic and ferrous iron pollutants to be drinking water standard following by WHO by co-precipitation process and membrane separation process. The relative effect of different solid media types (scouring sponge, scouring pad, plastic ring, and activated carbon foam) and operating conditions on oxygen mass transfer coefficient (KLa) and hydrodynamic bubble parameters was studied. The optimization process of ferrous oxidation and arsenic removal was observed by Design of Experiment (DOE) with Central Composition Design of Response Surface Methodology (CCD-RSM). Lastly, separation process was experimented with conventional process and membrane technology, i.e., settling process, …

Analysis Of Inventory In A Food Repackaging Company, Chutipapha Thiantravan

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aims to evaluate the nature of business and apply a suitable inventory policy to a case study food-repackaging company. The company adopted bulk-purchasing to packaging inventory to realize the economy of scales. Because of the lacks of purchasing standard and monitoring, this inventory policy led to the overstock, deadstock, and obsolete packaging inventories as well as the fluctuation of inventory turnover rate. The further category analysis of the packaging inventories using ABC analysis reveals that special customized bags and packaging items have a long lead time due to international sourcing and high minimum order quantity (MOQ). As a …

Assessments Of Chromium Pollution In Surface Sediment Of The Inner Gulf Of Thailand, Patcha Leelakun

Chulalongkorn University Theses and Dissertations (Chula ETD)

The inner Gulf of Thailand is an important marine resource for economic and social developments of Thailand. Moreover, the inner Gulf of Thailand is a diverse of biological coastal ecosystem, which is make an equilibrium marine environment. On the other hand, this area is due mainly to a variety of pollutant sources from the surface runoff, particularly major large rivers including the Mae Klong, the Tha Chin, the Chao Phraya and the Bangpakong Rivers. As a result, chromium contamination can occur in the inner Gulf of Thailand and ultimately become a serious environmental issue in Thailand. Therefore, the surface sediments …

Influence Of Seasonal Variations On Contamination Level And Ecological Risk Of Heavy Metals In The Surface Sediments Of The Inner Gulf Of Thailand, Phuttiphat Lapat-Atiwat

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Inner Gulf of Thailand is a marine resource that provides ecological and economic value. Meanwhile, its can be acted like pollutions sink, especially heavy metals. After that, the extensions of industrialization, agriculturalization, and urbanization can degenerate to the inner Gulf environment quality. Therefore, this thesis was aimed to study the spatial distribution and seasonal variation of heavy metal concentration (Cd, Pb, Cu, Co, Ni, Mn and Zn) in surface sediment including the Mae Klong, the Tha Chin, the Chao Phraya and the Bangpakong River estuaries. Moreover, the contamination status and ecological risk were also evaluated. The results shown the …