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High-Level Expression Of A Heterologous Protein In The Milk Of Transgenic Swine Using The Cdna Encoding Human Protein C, William H. Velander, John L. Johnson, Raymond L. Page, Christopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittius, William N. Drohan

William H. Velander Publications

Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 pg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from …

Utilizing Instructional Design Constructs To Enhance Computer-Based Instruction In Air Traffic Controller Training, Theodore Scott Rhoads

Master's Theses - Daytona Beach

This study demonstrates that a computer-based instructional design format is appropriate for novice air traffic control (ATC) training. The computer presents the student with small amounts of information in tutorial and drill and practice sequences that necessitate student interactivity. The student is then periodically audit tested to ascertain mastery of the subject matter. The result of these mid-lesson evaluations serve as a self-check so that the student can assess his/her progress in the learning cycle. The systematic design of instruction and interactive approach to learning, enhances student motivation, improves learning and retention, while placing the responsibility of achieving specific learning …

An Investigation Of Performance Appraisal With Recommendations For White Collar And Blue Collar Workers, Mark W. Alberts

Graduate Research Papers

The accurate evaluation of an employee's performance, whether it be a salary or wage worker, is one of the most challenging problems confronting any organization. According to Lazer (1977), which is still true today, performance appraisal is absolutely necessary if an organization is to prosper in the highly competitive world of modern business.

Regardless of all that has been written about the how and why of performance appraisal, there still seems to be constant problems and areas of clear disagreement. Published writings about performance appraisal cover approximately the past 30 years and deal mostly with private corporations. Before that, performance …

Selection And Implementation Of A Cad/Software System For Navigator Boats, Brian J. Finzel

Honors Capstone Projects and Theses

No abstract provided.

Acuta Enews December 1992, Vol. 21, No. 12

ACUTA Newsletters

In This Issue

Regulatory update

President's column

Dual routing protects Duke

Talking clock "tells" time

Seminar ratings

Director's column

High-Level Expression Of A Heterologous Protein In The Milk Of Transgenic Swine Using The Cdna Encoding Human Protein C, William H. Velander, John L. Johnson, Raymond L. Page, Chritopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittus, William N. Drohan

Papers in Biotechnology

Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 pg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from …

Excitation And De-Excitation Mechanisms Of Er-Doped Gaas And Algaas, David W. Elsaesser

Theses and Dissertations

Electrical and optical characterization have been performed on GaAs and Al_xGa_1-xAs samples doped with Er either by ion implantation or during Molecular Beam Epitaxial (MBE) growth. Deep Level Transient Spectroscopy (DLTS) and Temperature-Dependent Hall Effect (TDH) measurements indicated the presence of two hole traps in Er-doped GaAs, at 35 and 360 meV above the valence band maximum. The former (shallower) center was thought to be due to Er substituting for a Ga atom (Er_Ga) and giving rise to an isoelectronic impurity potential. The second center was attributed to an Er atom occupying an interstitial …

Solving Nonlinear Composite Material Problems Using The Automated Structural Optimization System (Astros), Robert Bolha

Theses and Dissertations

Most advanced composite materials have nonlinear stress versus strain curves--grossly so in the laminas shear direction. If linear approximations are used, overly conservative design will result. This thesis expands the usefulness of the Automated Structural Optimization System (ASTROS) finite element (FE) program by incorporating a newly developed capability to include material nonlinearities. By dividing a FE problem into several intervals, and incorporating the changing nature of the nonlinear material properties within each element, a good approximation of the laminate's actual stresses and strains can be obtained without the undue experimentation involved in testing every individual configuration. Each material property is …

An Electromagnetically-Controlled Precision Orbital Tracking Vehicle (Potv), Richard E. Lawrence Jr.

Theses and Dissertations

A propulsion configuration it defined for a precision orbital tracking vehicle (POTV) that employs electrodynamic forces to control vehicle attitude and position with respect to another spacecraft. A pair of electrically powered thrusters assist the POTV in position control. The vehicle can maintain continuous standoff at close range from another spacecraft in any direction, has docking capability, and has gross orbital transfer capability. These capabilities and the specific design derived for the POTV have been defined by the requirements of a postulated space facility (ASSET) that salvages structural aluminum from the external fuel tank of the space shuttle. Precision control …

Characterization Of Stall Inception In High-Speed Single-Stage Compressors, Keith M. Boyer

Theses and Dissertations

Two single-stage, transonic compressor designs were tested under various undistorted operating conditions to characterize the process leading up to aerodynamic stall. The rig case was instrumented with eight high-response static pressure transducers equally spaced around the annulus for stall development detection. High-response measurements were low-pass filtered and both spatially and temporally analyzed using discrete Fourier techniques. At all speeds tested for both designs, stall inception was characterized by growth of a small amplitude' rotating wave. The waves did not grow significantly until just prior to the instability, when exponential growth into fully-developed rotating stall occurred very rapidly, within 6-10 rotor …

Experimental Investigation Of The Aerodynamics Of Independently Rotating Cylindrical Shells, Walter C. Howerton

Theses and Dissertations

The purpose of this thesis was to investigate experimentally the aerodynamic forces on two adjacent, independently rotating cylinders in a cross- flow. This investigation involved a force measurement setup which had the two cylinders mounted on a common support shaft, extending beyond the span of the wind tunnel test section, and supported by spoke rings with strain gages in the vertical and horizontal directions. This setup proved capable of measuring aerodynamic forces. The lift on the independently rotating cylinders was found to increase as one cylinder's angular speed increased while the other's remained constant, and decrease as the relative angular …

Helicopter Flight Control System Design Using The Linear Quadratic Regulator For Robust Eigenstructure Assignment, Dempsey D. Solomon

Theses and Dissertations

This thesis applied modern, multi-variable control design techniques, via a FORTRAN computer algorithm, to U.S. Army helicopter models in hovering flight conditions. Eigenstructure assignment and Linear Quadratic Regulator (LQR) theory are used to achieve enhanced closed loop performance and stability characteristics with full state feedback. The addition of cross coupling weights to the standard LQR performance index is specifically addressed. A desired eigenstructure is chosen with a goal of reduced pilot workload via performance qualities requirements. Cross coupling weighting is shown to provide greater flexibility in achieving a desired closed loop eigenstructure. While the addition of cross coupling weighting is …

Object-Oriented Analysis, Design, And Implementation Of The Saber Wargame, David S. Douglass

Theses and Dissertations

Saber is a two-sided, air and land war game that simulates decisions made of commanders at the theater-level. It is being developed by the Air Force Institute of Technology for the Air Force Wargaming center at Maxwell AFB, Alabama. Saber models conventional, chemical, and nuclear warfare between aggregated air and land forces. It also portrays the effects of logistics, satellites, weather, terrain, and intelligence which add to the realism of the Saber war game. The Saber war game has three main components, the preprocessor. which is responsible for scenario development and pregame activities, the simulation. the guts of the war …

Design And Development Of A High-Speed Winograd Fast Fourier Transform Processor Board, James F. Herron

Theses and Dissertations

Since 1985, the Air Force Institute of Technology has pursued a project to develop a 4080-point Discrete Fourier Transform processor using the Winograd Fourier Transform Algorithm (WFTA) and Good-Thomas Prime Factoring Algorithm (PFA). In the first attempt to build a working system, this research effort designed and constructed, in part, a modified single processor architecture in order to demonstrate the proof of concept of the WFTA system design. This prototype architecture is simpler in implementation but uses the same principles and procedures as those of the 4080-point WFTA design. The design developed in this thesis was validated using the Very …

Solution To A Multicriteria Aircraft Routing Problem Utilizing Parallel Search Techniques, James J. Grimm Iii

Theses and Dissertations

Pilots select routes based on factors such as threats, fuel, time on target, distance, and refueling points. This is a time consuming task. This thesis presents the software engineering synthesis of a software tool, based on a parallelized A* search algorithm, to select routes. For simplicity only threats and distance are used. A centralized open list is used with one processor managing the list while the other processors perform the node expansions. This decomposition result in a dynamically load balanced system. A number of parameters are changed to study their impact on the execution time. The use of a branch …

Generalization And Parallelization Of Messy Genetic Algorithms And Communication In Parallel Genetic Algorithms, Laurence D. Merkle

Theses and Dissertations

Genetic algorithms (GA) are highly parallelizable, robust semi- optimization algorithms of polynomial complexity. The most commonly implemented GAs are 'simple' GAs (SGAs). Reproduction, crossover, and mutation operate on solution populations. Deceptive and GA-hard problems are provably difficult for simple GAs. Messy GAs (MGA) are designed to overcome these limitations. The MGA is generalized to solve permutation type optimization problems. Its performance is compared to another MGA's, an SGA's, and a permutation SGA's. Against a fully deceptive problem the generalized MGA (GMGA) consistently performs better than the simple GA. Against an NP-complete permutation problem, the GMGA performs better than the other …

An Intelligent Real-Time System Architecture Implemented In Ada, Michael A. Whelan

Theses and Dissertations

Conventional real-time systems are fully deterministic allowing for off-line, optimal, task scheduling under all circumstances. Real-time intelligent systems add non-deterministic task execution times and non- deterministic task sets for scheduling purposes. Non-deterministic task sets force intelligent real-time systems to trade-off execution time with solution quality during run-time and perform dynamic task scheduling. Four basic design considerations addressing those tradeoffs have been identified: control reasoning, focus of attention, parallelism, and algorithm efficacy. Non-real- time intelligent systems contain an environment sensor, a model of the environment, a reasoning process, and a large collection of procedural processes. Real-time intelligent systems add to these …

A Synthetic Environment For Satellite Modeling And Satellite Orbital Motion, David L. Pond

Theses and Dissertations

Analysts tasked to study satellites moving in space must often rely on photographs to conduct their analysis. Often these photographs do not adequately represent how satellites move in space, their spatial relationships, or their interaction with other satellites. What analysts need is a three- dimensional synthetic environment that depicts satellites as they actually move and interact in space. This thesis addresses the early development of the Satellite Modeler (SM), a system that allows analysts to study, satellite motion and maneuvering of computer aided design (CAD) models of those satellites in a three-dimensional orbit. The system contains equipment that allows analysts …

A Synthetic Environment Flight Simulator: The Afit Virtual Cockpit, John C. Switzer

Theses and Dissertations

This thesis describes the design and implementation of a part of the Virtual Cockpit: a synthetic environment, distributed network flight simulator. The goal of the project was to prove the concept that this type of flight simulator could fill the gap between high-end, very expensive flight simulators and low-end game quality flight simulators. Discussed are: object-oriented design techniques, multi-processor utilization, the flight dynamics model, synthetic environment technology, the frame-rate vs. realism issue, and the interfaces to a realistic joystick and throttle.

Development Of A Protocol Usage Guideline For Conservative Parallel Simulations, Prescott J. Van Horn

Theses and Dissertations

The objective of distributed simulation is to speedup simulation execution by partitioning the simulation processing load over multiple processors, This thesis reviews current synchronization protocol methods for distributed simulations, and proposes guidelines for obtaining optimal conservative simulation partitionings using empirical evidence. An analysis is performed using three protocol variations of the Chandy-Misra HULL message algorithm, two using a pending message blocking strategy, and the other using a safetime blocking strategy. A fourth protocol evaluated is based on the SRADS algorithm proposed by Reynolds. The analysis involves a study of all possible 2 and 4 node configurations, for three queuing simulations, …

Formalizing, Validating, And Verifying Real-Time System Requirements With Reacto And Vhdl, Frank C. Young

Theses and Dissertations

We develop a methodology for formalizing, verifying, and validating the requirements specification of real-time systems based on a graphical and formal hierarchical Finite State Machine (FSM) language Reacto. We define a means to quantify time and express real-time constraints in Reacto and a transformation from Reacto to the Very High Speed Integrated Circuit (VHSIC) hardware Description Language (VHDL). Reacto's high level abstractions, graphical nature, and theorem prover produce efficient, accurate, and easily understood specifications. We use VHDL's event driven simulation capability, concurrency, and temporal operators to thoroughly examine temporal dependencies between the state machine transitions, and to increase simulation power …

Cepstral And Auditory Model Features For Speaker Recognition, John M. Colombi

Theses and Dissertations

The TIMIT and KING databases, as well as a ten day AFIT speaker corpus, are used to compare proven spectral processing techniques to an auditory neural representation for speaker identification. The feature sets compared were Linear Predictive Coding (LPC) cepstral coefficients and auditory nerve firing rates using the Payton model. This auditory model provides for the mechanisms found in the human middle and inner auditory periphery as well as neural transduction. Clustering algorithms were used to generate speaker specific codebooks - one statistically based and the other a neural approach. These algorithms are the Linde-Buzo-Gray (LBG) algorithm and a Kohonen …

Satcom General Purpose Modem Dscs Iii Sct Beacon Telemetry Display, James D. Coppola

Theses and Dissertations

This thesis documents the design and implementation of a DSCS III single Channel Transponder (SCT) beacon telemetry display. The system is a personal computer based design which interfaces to three SCT beacon receiver/demodulators. The software was designed to decode and display both the DSCS III A and DSCS HI B satellite beacons. Recordings of the SCT beacon display can be made on paper and/or magnetic media when triggered by the user, a watchdog timer, or the SCT command accept telemetry bit. In addition, the system can be configured with an IRIG B Universal Time Coordinates (UTC) card which enables the …

An Analytical And Experimental Investigation Of Fm-By-Noise Jamming, Charles J. Daly

Theses and Dissertations

Among the jamming waveforms used in Electronic Warfare, FM-by-noise is the least understood, and no exhaustive analytical and experimental investigation of the subject exists. This investigation presents a thorough review, consolidation, and elucidation of the theory of FM-by-noise. To explain and predict the behavior of FM-by-noise at RF and IF, three useful ratios, namely the deviation-to-victim ratio, the noise-to-victim ratio, and the sweep- to-victim ratio, are developed. To complement the theory, results of FM-by-noise jamming experiments conducted using commercial test and measurement equipment are presented. Specifically, the time- and frequency-domain behavior of FM-by- noise at RF and IF is studied …

Analysis And Simulation Of Modified Tanlock And Delay Lock Loops For Gps Receiver Design, James A. Hird

Theses and Dissertations

The purpose of this thesis is to investigate two types of tracking loops used in Global Positioning System (GPS) receiver design. The first loop, the Delay Lock Loop (DLL), is a code tracking loop used to synchronize a locally generated pseudo-noise (PN) sequence with the PN sequence in the GPS satellite broadcast. Synchronization of the PN sequences is essential for de-spreading the direct-sequence spread spectrum (DS/SS) broadcast and demodulating the transmitted data. The second loop, the Modified Tanlock Loop (MTLL), is a carrier tracking loop used to synchronize the phase of a voltage controlled oscillator (VCO) with the carrier of …

Face Recognition With Neural Networks, Dennis L. Krepp

Theses and Dissertations

This study investigated neural networks for face verification and classification. The research concentrated on developing a neural network based feature extractor and/or classifier to perform authorized user verification in a realistic work environment. Recognition accuracy, system assumptions, training time, and execution time were analyzed to determine the feasibility of a neural network approach. Data was collected using a camcorder and two segmentation schemes: manual segmentation and motion-based, automatic segmentation. Data consisted of over 2000. 32x32 pixel, 8 bit gray scale images of 52 subjects; each subject had two to ten days worth of images collected. Several training and test sets …

Computer Simulation Of A General Purpose Satellite Modem, William L. Montgomery Jr.

Theses and Dissertations

The purpose of this research was to model and simulate the performance of a digital phase shift keyed satellite modem. The probability of bit error (P sub b) at different levels of energy per bit to noise power ratio (E sub b/N sub o) was the performance measure. The channel was assumed to contribute only additive white Gaussian noise. A second order Costas loop performs demodulation in the modem and was the key part of the simulation. The Costas loop with second order Butterworth arm filters was tested by finding the response to a phase or frequency step. The Costas …

Digital Signal Processing Using Lapped Transforms With Variable Parameter Windows And Orthonormal Bases, Brian D. Raduenz

Theses and Dissertations

This thesis develops and evaluates a number of new concepts and tools for the analysis of signals using variable overlapped windows and orthonormal bases. Windowing, often employed as a spectral estimation technique, can result in irreparable distortions in the transformed signal. By placing conditions on the window and incorporating it into the orthonormal representation, any signal distortion resulting from the transformation can be eliminated or cancelled in reconstruction. This concept is critical to the theory underpinning this thesis. As part of this evaluation, a tensor product based general N-point fast Fourier transform algorithm was implemented in the DOD standard language, …

Automated Face Recognition System, Kenneth R. Runyon

Theses and Dissertations

In this thesis three variations of an end-to-end face recognition prototype system are developed, implemented and tested. Each version includes real-time image collection, automated segmentation, preprocessing, feature extraction, and classification. The first version uses a Karhunen Loeve Transform (KLT) feature extractor and a K-nearest neighbor classifier. Version two uses the same feature set but utilizes a multilayer perception neural network with a back propagation learning rule. Finally the third version uses a Discrete Cosine Transform as the feature extractor and the K-nearest neighbor as the classifier. Only the KLT versions of the system were tested. The tests were based on …

Enhancement Of Audio Localization Cue Synthesis By Adding Environmental And Visual Cues, Eric L. Scarborough

Theses and Dissertations

An audio localization cue synthesizer, the DIRectional Audio Display (DIRAD) was used to simulate auditory distance, room reflections, and to provide spatial audio for computer graphics images. The DIRAD processes input audio signals to generate spatially located sounds for headphone listening. The DIRAD can position audio sources around the head and these sounds are stable with respect to the listener's head position. An interactive, real-time simulation of auditory distance and room reflections was accomplished using the DIRAD in combination with a Silicon Graphics audio processor board installed in a Personal Iris 4D/35. Several demonstrations of auditory distance and the effects …