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Development, Design, Manufacture And Test Of Flapping Wing Micro Aerial Vehicles, Todd J. Smith Jan 2016

Development, Design, Manufacture And Test Of Flapping Wing Micro Aerial Vehicles, Todd J. Smith

Browse all Theses and Dissertations

The field of FlappingWing Micro Air Vehicles (FWMAV) has been of interest in recent years and as shown to have many aerodynamic principles unconventional to traditional aviation aerodynamics. In addition to traditional manufacturing techniques, MAVs have utilized techniques and machines that have gained significant interest and investment over the past decade, namely in additive manufacturing. This dissertation discusses the techniques used to manufacture and build a 30 gram-force (gf) model which approaches the lower limit allowed by current commercial off-the-shelf items. The vehicle utilizes a novel mechanism that minimizes traditional kinematic issues associated with four bar mechanisms for flapping wing …

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Distinct And Shared Determinants Of Cardiomyocyte Contractility In Multi-Lineage Competent Ethnically Diverse Human Ipscs, Martin L. Tomov, Zachary T. Olmsted, Haluk Dogan, Eda Gongorurler, Maria Tsompana, Hasan H. Otu, Michael Buck, Eun-Ah Chang, Jose Cibelli, Janet L. Paluh Jan 2016

Distinct And Shared Determinants Of Cardiomyocyte Contractility In Multi-Lineage Competent Ethnically Diverse Human Ipscs, Martin L. Tomov, Zachary T. Olmsted, Haluk Dogan, Eda Gongorurler, Maria Tsompana, Hasan H. Otu, Michael Buck, Eun-Ah Chang, Jose Cibelli, Janet L. Paluh

Department of Electrical and Computer Engineering: Faculty Publications

The realization of personalized medicine through human induced pluripotent stem cell (iPSC) technology can be advanced by transcriptomics, epigenomics, and bioinformatics that inform on genetic pathways directing tissue development and function. When possible, population diversity should be included in new studies as resources become available. Previously we derived replicate iPSC lines of African American, Hispanic-Latino and Asian self-designated ethnically diverse (ED) origins with normal karyotype, verified teratoma formation, pluripotency biomarkers, and tri-lineage in vitro commitment. Here we perform bioinformatics of RNA-Seq and ChIP-seq pluripotency data sets for two replicate Asian and Hispanic-Latino ED-iPSC lines that reveal differences in generation of …

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Validation Of Integrated Coast-Ocean Model Cche2d-Coast And Development Of Wind Model, Afshin Gazerzadeh Jan 2016

Validation Of Integrated Coast-Ocean Model Cche2d-Coast And Development Of Wind Model, Afshin Gazerzadeh

Electronic Theses and Dissertations

The eastern and southern coastlines of the United States are two of the most cyclone-prone areas of the world. The effects of tropical cyclones vary mainly depending on wind intensity and geological features of the coast that it is crossing. Higher winds potentially generate higher storm surges and consequently larger floods occur along the coastlines. Therefore, it is critical to accurately predict winds, storm surge, and waves associated with a hurricane. In the present study, an integrated coastal and ocean process model, CCHE2D-Coast, is validated by assessing the model’s capabilities in simulating coast-ocean circulations driven by the astronomical tides on …

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Novel Dynamic Partial Reconfiguration Implementations Of The Support Vector Machine Classifier On Fpga, Hanaa Hussain, Khaled Benkrid, Hüseyi̇n Şeker Jan 2016

Novel Dynamic Partial Reconfiguration Implementations Of The Support Vector Machine Classifier On Fpga, Hanaa Hussain, Khaled Benkrid, Hüseyi̇n Şeker

Turkish Journal of Electrical Engineering and Computer Sciences

The support vector machine (SVM) is one of the highly powerful classifiers that have been shown to be capable of dealing with high-dimensional data. However, its complexity increases requirements of computational power. Recent technologies including the postgenome data of high-dimensional nature add further complexity to the construction of SVM classifiers. In order to overcome this problem, hardware implementations of the SVM classifier have been proposed to benefit from parallelism to accelerate the SVM. On the other hand, those implementations offer limited flexibility in terms of changing parameters and require the reconfiguration of the whole device. The latter interrupts the operation …

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A Computational Study Of The Evolution Of Cretan And Related Scripts, Peter Revesz Oct 2015

A Computational Study Of The Evolution Of Cretan And Related Scripts, Peter Revesz

School of Computing: Conference and Workshop Papers

Crete was the birthplace of several ancient writings, including the Cretan Hieroglyphs, the Linear A and the Linear B scripts. Out of these three only Linear B is deciphered. The sound values of the Cretan Hieroglyph and the Linear A symbols are unknown and attempts to reconstruct them based on Linear B have not been fruitful. In this paper, we compare the ancient Cretan scripts with four other Mediterranean and Black Sea scripts, namely Phoenician, South Arabic, Greek and Old Hungarian. We provide a computational study of the evolution of the three Cretan and four other scripts. This study encompasses …

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Bioprinting As A Tool To Evaluate Interactions Of Cancerous And Noncancerous Cells, Holly Jordan Aug 2015

Bioprinting As A Tool To Evaluate Interactions Of Cancerous And Noncancerous Cells, Holly Jordan

All Theses

Cancer is becoming one the leading causes of death worldwide, and in particular, breast cancer, which is the second highest cause of cancer death for women. Approximately 12 percent of US women will develop invasive breast cancer, and about 40,000 of US women will die from breast cancer in 2015. With better detection and treatment options, breast cancer death has been decreasing over the past two decades. Despite declining rates in breast cancer death, cancer progression is not well understood. There are many studies focused on cancer, but in situ cancer studies are often hard to reproduce and can even …

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Silver Nanoparticle Coated Bioactive Glasses - Composites With Dex/Cmc Hydrogels: Characterization, Solubility, And In Vitro Biological Studies, Anthony W. Wren, Pegah Hassanzadeh, Lana M. Placek, Timothy J. Keenan, Aisling Coughlan, Lydia R. Boutelle, Mark R. Towler Aug 2015

Silver Nanoparticle Coated Bioactive Glasses - Composites With Dex/Cmc Hydrogels: Characterization, Solubility, And In Vitro Biological Studies, Anthony W. Wren, Pegah Hassanzadeh, Lana M. Placek, Timothy J. Keenan, Aisling Coughlan, Lydia R. Boutelle, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

Silver (Ag) coated bioactive glass particles (Ag-BG) were formulated and compared to uncoated controls (BG) in relation to glass characterization, solubility and microbiology. X-ray diffraction (XRD) confirmed a crystalline AgNP surface coating while ion release studies determined low Ag release (/L). Cell culture studies presented increased cell viability (127 and 102%) with lower liquid extract (50 and 100 ml/ml) concentrations. Antibacterial testing of Ag-BG in E. coli, S. epidermidis and S. aureus significantly reduced bacterial cell viability by 60-90%. Composites of Ag-BG/CMC-Dex Hydrogels were formulated and characterized. Agar diffusion testing was conducted where Ag-BG/hydrogel composites produced the largest inhibition zones …

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A Non-Invasive Fluorescence-Based Oxygen Sensor And Platform For Studying Cell Responses To Metabolic Agents In Real-Time, Koutilya Reddy Buchapudi Jul 2015

A Non-Invasive Fluorescence-Based Oxygen Sensor And Platform For Studying Cell Responses To Metabolic Agents In Real-Time, Koutilya Reddy Buchapudi

Doctoral Dissertations

A fluorescence-based sensor in a transverse flow/stop measurement platform has been developed to determine real-time changes in oxygen consumption rates for cell metabolic studies. The oxygen sensitive fluorophore platinum octaethylporphyrin was embedded in a cellulose acetate matrix and affixed to a fiber optic bundle, which provided for transmission of the excitation and emission wavelengths of the film. The fiber optic bundle was sealed in a sensor head that can be used in standard 24-well plates common to research labs. The utility of the sensor and sensing platform were determined by measuring the changes in oxygen consumption rates of Candida albicans …

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Molecular Dynamics Investigation Of The Arabinan-Cellulose Interface For Cellulose Nanocomposite Applications, Luke Thornley Jun 2015

Molecular Dynamics Investigation Of The Arabinan-Cellulose Interface For Cellulose Nanocomposite Applications, Luke Thornley

Materials Engineering

Atom level computer simulations of the arabinan and cellulose interface were performed to better understand the mechanisms that give arabinan-cellulose composites (ArCCs) their strength with the goal to improve man-made ArCCs. The molecular dynamics (MD) software LAMMPS was used in conjunction with the ReaxFF/c force field to model the bond between cellulose and arabinan. A cellulose nanocrystal with dimensions 51 x 32 x 8 Å was minimized with various weight percent of water, 0%, 3%, 5%, 8%, 10%, and 12%. After the system was equilibrated for at least 100,000 femtoseconds, an arabinan molecule composed of 8 arabinose rings was added …

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Selection Methods For Genetically-Modified T Cells: In Support Of Translational Therapy, David Rushworth May 2015

Selection Methods For Genetically-Modified T Cells: In Support Of Translational Therapy, David Rushworth

Dissertations and Theses (Open Access)

T cells are blood cells which organize the immune system of the host. These cells are necessary for the host to respond appropriately to threats from foreign organisms and cancerous growth. However, in the case of certain infections and cancer, T cells are unable to respond appropriately to a threat and establish immunity. This leads to disease when the infection or cancer is not sufficiently eliminated. On the other hand, T cells can lack tolerance for healthy tissue and perceive healthy tissue as infected. The ensuing over-reactive immune response also leads to disease. A delicate balance must exist between immunity …

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Magnetic Manipulation And Multimodal Imaging For Single Cell Direct Mechanosensing, Robert L. Wilson Apr 2015

Magnetic Manipulation And Multimodal Imaging For Single Cell Direct Mechanosensing, Robert L. Wilson

Open Access Theses

The study of internal mechanics of single cells is paramount to understand mechanisms of mechanoregulation. External loading and cell-mediated force generation result in changes in cell shape, rheology, and the deformation of subcellular structures such as the nucleus. Moreover, alterations in the processes that regulate these responses have been further correlated to specific pathologies. Cellular deformation is often studied through application of forces in the environment of the cell, relying on strain and stress transfer through focal adhesions and the cytoskeletal system. However, the transfer of these external forces to internal mechanics can introduce uncertainties in the interpretation of subcellular …

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Generation Of Multicellular Tumor Spheroids With Microwell-Based Agarose Scaffolds For Drug Testing, Xue Gong, Chao Lin, Jian Cheng, Jiansheng Su, Hang Zhao, Tianlin Liu, Xuejun Wen, Peng Zhao Jan 2015

Generation Of Multicellular Tumor Spheroids With Microwell-Based Agarose Scaffolds For Drug Testing, Xue Gong, Chao Lin, Jian Cheng, Jiansheng Su, Hang Zhao, Tianlin Liu, Xuejun Wen, Peng Zhao

Chemical and Life Science Engineering Publications

Three dimensional multicellular aggregate, also referred to as cell spheroid or microtissue, is an indispensable tool for in vitro evaluating antitumor activity and drug efficacy. Compared with classical cellular monolayer, multicellular tumor spheroid (MCTS) offers a more rational platform to predict in vivo drug efficacy and toxicity. Nevertheless, traditional processing methods such as plastic dish culture with nonadhesive surfaces are regularly time-consuming, laborious and difficult to provide uniform-sized spheroids, thus causing poor reproducibility of experimental data and impeding high-throughput drug screening. In order to provide a robust and effective platform for in vitro drug evaluation, we present an agarose scaffold …

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Generation Of Multicellular Tumor Spheroids With Microwell-Based Agarose Scaffolds For Drug Testing, Xue Gong, Chao Lin, Jian Cheng, Jiansheng Su, Hang Zhao, Tianlin Liu, Xuejun Wen, Peng Zhao Jan 2015

Generation Of Multicellular Tumor Spheroids With Microwell-Based Agarose Scaffolds For Drug Testing, Xue Gong, Chao Lin, Jian Cheng, Jiansheng Su, Hang Zhao, Tianlin Liu, Xuejun Wen, Peng Zhao

Chemical and Life Science Engineering Publications

Three dimensional multicellular aggregate, also referred to as cell spheroid or microtissue, is an indispensable tool for in vitro evaluating antitumor activity and drug efficacy. Compared with classical cellular monolayer, multicellular tumor spheroid (MCTS) offers a more rational platform to predict in vivo drug efficacy and toxicity. Nevertheless, traditional processing methods such as plastic dish culture with nonadhesive surfaces are regularly time-consuming, laborious and difficult to provide uniform-sized spheroids, thus causing poor reproducibility of experimental data and impeding high-throughput drug screening. In order to provide a robust and effective platform for in vitro drug evaluation, we present an agarose scaffold …

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Liver Sinusoid On A Chip: Long-Term Layered Co-Culture Of Primary Rat Hepatocytes And Endothelial Cells In Microfluidic Platforms, Young Bok (Abraham) Kang, Temitope R. Sodunke, Jason Lamontagne, Joseph Cirillo, Caroline Rajiv, Michael J. Bouchard, Moses Noh Jan 2015

Liver Sinusoid On A Chip: Long-Term Layered Co-Culture Of Primary Rat Hepatocytes And Endothelial Cells In Microfluidic Platforms, Young Bok (Abraham) Kang, Temitope R. Sodunke, Jason Lamontagne, Joseph Cirillo, Caroline Rajiv, Michael J. Bouchard, Moses Noh

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

We describe the generation of microfluidic platforms for the co-culture of primary hepatocytes and endothelial cells; these platforms mimic the architecture of a liver sinusoid. This paper describes a progressional study of creating such a liver sinusoid on a chip system. Primary rat hepatocytes (PRHs) were co-cultured with primary or established endothelial cells in layers in single and dual microchannel configurations with or without continuous perfusion. Cell viability and maintenance of hepatocyte functions were monitored and compared for diverse experimental conditions. When primary rat hepatocytes were co-cultured with immortalized bovine aortic endothelial cells (BAECs) in a dual microchannel with continuous …

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Microbubble Generation By Piezotransducer For Biological Studies, W. Zhu, M. Alkhazal, M. Cho, S. Xiao Jan 2015

Microbubble Generation By Piezotransducer For Biological Studies, W. Zhu, M. Alkhazal, M. Cho, S. Xiao

Bioelectrics Publications

Bubbles induced by blast waves or shocks are speculated to be the major cause of damages in biological cells in mild traumatic brain injuries. Microbubble collapse was found to induce noticeable cell detachment from the cell substrate, changes in focal adhesion and biomechanics. To better understand the bubble mechanism, we would like to construct a system, which allows us to clearly differentiate the impact of bubbles from that of shocks. Such a generator needs to be low profile in order to place under a microscope. A piezoelectric transducer system was designed to meet the need. The system uses either a …

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Mammalian Synthetic Biology: Emerging Medical Applications, Zoltan Kis, Hugo Sant'ann Pereira, Takayuki Homma, Ryan M. Pedrigi, Rob Krams Jan 2015

Mammalian Synthetic Biology: Emerging Medical Applications, Zoltan Kis, Hugo Sant'ann Pereira, Takayuki Homma, Ryan M. Pedrigi, Rob Krams

Department of Mechanical and Materials Engineering: Faculty Publications

In this review, we discuss new emerging medical applications of the rapidly evolving field of mammalian synthetic biology.We start with simple mammalian synthetic biological components and move towards more complex and therapy-oriented gene circuits. A comprehensive list of ON–OFF switches, categorized into transcriptional, post-transcriptional, translational and posttranslational, is presented in the first sections. Subsequently, Boolean logic gates, synthetic mammalian oscillators and toggle switches will be described. Several synthetic gene networks are further reviewed in the medical applications section, including cancer therapy gene circuits, immuno-regulatory networks, among others. The final sections focus on the applicability of synthetic gene networks to drug …

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Brown Adipogenesis Of Mouse Embryonic Stem Cells In Alginate Microstrands, Andrea Mannarino Unser Jan 2015

Brown Adipogenesis Of Mouse Embryonic Stem Cells In Alginate Microstrands, Andrea Mannarino Unser

Legacy Theses & Dissertations (2009 - 2024)

The ability of brown adipocytes (fat cells) to dissipate energy as heat shows great promise for the treatment of obesity and other metabolic disorders. Employing pluripotent stem cells, with an emphasis on directed differentiation, may overcome many issues currently associated with primary fat cell cultures. However, brown adipocytes are difficult to transplant in vivo due to the instability of fat, in terms of necrosis and neovascularization, once injected. Thus, 3D cell culture systems that have the potential to mimic adipogenic microenvironments are needed, not only to advance brown fat implantation, but also to better understand the role of brown adipocytes …

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3d-Bioengineering Of The Conventional Outflow Tract For High Throughput Drug Or Gene Transfer Screening For Glaucoma Treatment, Cula Nolise Dautriche Jan 2015

3d-Bioengineering Of The Conventional Outflow Tract For High Throughput Drug Or Gene Transfer Screening For Glaucoma Treatment, Cula Nolise Dautriche

Legacy Theses & Dissertations (2009 - 2024)

Among ocular pathologies, glaucoma remains the second leading cause of blindness. The molecular mechanisms that lead to glaucoma have been attributed to damage of the conventional outflow tract. Conventional outflow tissues, a composite of the trabecular meshwork and the Schlemm's canal, regulate and maintain homeostatic responses of aqueous humor outflow. In glaucoma, drainage of aqueous humor into the Schlemm's canal is hindered, leading to an increase in intraocular pressure (IOP). That increase in IOP is directly correlated with retinal ganglion cell death, eliminating the relay of visual information to the thalamus and visual cortex, leading to blindness. Although disturbance in …

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Behavioral Operations Management In Federal Governance, Frederick Leonard Mobley Jan 2015

Behavioral Operations Management In Federal Governance, Frederick Leonard Mobley

Walden Dissertations and Doctoral Studies

The environmental uncertainty of federal politics and acquisition outsourcing in competitive markets requires an adaptive decision-analysis structure. Practitioners oriented toward exclusively static methods face severe challenges in understanding qualitative aspects of organizational governance. The purpose of this grounded theory study was to examine and understand behavioral relationship attributes within intuitive, choice, judgment, or preference decision-making processes. The problem addressed in this study was the detrimental effects of organizational citizenship behavior (OCB), compulsory citizenship behavior (CCB), and social exchange theory (SET) on the acquisition management relationship The OCB, CCB, SET dictates that sound business development, relationship acumen, emotional intelligence and perceptiveness …

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Programmable Dna Delivery To Cells Using Bioreducible Layer-By-Layer (Lbl) Polyelectrolyte Thin Films, Maria Muniz Jan 2015

Programmable Dna Delivery To Cells Using Bioreducible Layer-By-Layer (Lbl) Polyelectrolyte Thin Films, Maria Muniz

Wayne State University Theses

Bioreducible, layer-by-layer (LbL) polyelectrolyte films show great promise for use in biomedical implant and gene-delivery applications. These nanometer-scale thin films can easily be coated onto a variety of implantable surfaces or devices. To achieve proof of concept, HEK-293, NIH/3T3, RAW, and MC3T3 cells were transfected using plasmid DNA with the green fluorescent protein reporter gene (GFP-DNA). In order to optimize transfection, a number of polyelectrolytes and biological components were systematically incorporated into the two similar basic LbL structures. Based on our previous degradation assays, it is shown that cellular interactions in vitro can break down the layers of the thin …

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Observing, Understanding, And Manipulating Biological Membranes, Ian Mc Cabe Jan 2015

Observing, Understanding, And Manipulating Biological Membranes, Ian Mc Cabe

Dissertations - ALL

The phospholipid bilayer is one of the hallmarks of eukaryotic life. This complicated two dimensionally fluid surface is composed of a double layer of lipids which have a region that is hydrophobic and a region that is hydrophilic. The lipid bilayer membranes of a cell act as a barrier that distinguishes the cells and the organelles interiors from the outside environment. In order for the cell to be able to effectively communicate across these impermeable barriers they have evolved many intricate systems of lipid and protein interaction that serve to transmit information from one side of the membrane to the …

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A Novel Function For Fancj Helicase In Microsatellite Stabilization During Replication Stress, Joanna Barthelemy Jan 2015

A Novel Function For Fancj Helicase In Microsatellite Stabilization During Replication Stress, Joanna Barthelemy

Browse all Theses and Dissertations

Noncanonical DNA structure-forming sequences, such as hairpin structures, stall replication forks in vivo, expand or contract during DNA replication, and colocalize with chromosome fragile sites. Since the frequency of hairpin formation is higher than the frequency of expansion or contraction, the cell may possess mechanisms to resolve hairpin structures prior to replication fork stalling. One possible activity for hairpin resolution is the FANCJ DNA helicase, known to unwind noncanoncial DNA structures in vitro. Indeed, mutations or loss of any of the nineteen FANC genes, including FANCJ, leads to genome instability and the cancer-prone syndrome Fanconi anemia (FA). To test whether …

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The Future Of Carbon-Based Scaffolds In Foot And Ankle Surgery, Jarema S. Czarnecki, Khalid Lafdi, Panagiotis A. Tsonis Jan 2015

The Future Of Carbon-Based Scaffolds In Foot And Ankle Surgery, Jarema S. Czarnecki, Khalid Lafdi, Panagiotis A. Tsonis

Chemical and Materials Engineering Faculty Publications

Autologous grafts have been the gold standard in tissue replacement and the most accurate means of recapitulating both the biological and mechanical properties of tissue. However, autologous grafts have had complications and drawbacks. Skin grafting, a prime example of an autologous tissue graft, has been limited by the size of graft, availability, and secondary donor site morbidity. Use of cadaveric tissues circumvents several limitations of autologous grafts; however, sterilization processes used to reduce the risk of disease transmission potentially weaken tissues and eliminate living cells and some growth factors from scaffolds, making them suboptimal tissue replacements. Chemical cross-linkage of tissue …

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Chemotaxis Of Central Nervous System-Derived Tumor Cells Within Controlled Microenvironments, Jennifer Rico Varela Jan 2015

Chemotaxis Of Central Nervous System-Derived Tumor Cells Within Controlled Microenvironments, Jennifer Rico Varela

Dissertations and Theses

Medulloblastoma (MB) is a malignant pediatric brain tumor known for its aggressive metastatic potential. Despite the well-documented migration of MB cells to other parts of the brain and spinal column, MB chemotaxis is poorly understood. Herein, we examined the in vitro migratory and cellular responses of MB-derived cells to external signaling of Epidermal Growth Factor (EGF), hepatocyte growth factor (HGF), platelet-derived growth factor (PDGF-BB), and the stromal cell-derived factors 1-alpha (SDF-1). Experiments utilized transwell assays and immunocytochemistry to identify receptor activation in MB migration, and used a microfluidic platform to examine directionality, trajectory, and gradient- dependence of motile cells. Data …

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Metabolic Engineering Of Cyanobacteria For Photosynthetic Production Of Drop-In Liquid Fuels, Bertram Michael Berla Dec 2014

Metabolic Engineering Of Cyanobacteria For Photosynthetic Production Of Drop-In Liquid Fuels, Bertram Michael Berla

McKelvey School of Engineering Graduate Student Theses & Dissertations

Cyanobacteria are oxygenic phototrophs with great potential as hosts for renewable fuel and chemical production. They grow very quickly (compared with plants) and can use sunlight for energy and CO2 as a carbon source (unlike yeast or E. coli). While cyanobacteria have been engineered to make many chemicals that are native and non-native parts of their metabolism, this work is concerned with the production of heptadecane in Synechocystis sp. PCC 6803. Heptadecane is in a class of natural products produced by all cyanobacteria, but in quantities insufficient for industrialization. Towards this future goal, we have built enabling systems for the …

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High Frequency Devices And Circuit Modules For Biochemical Microsystems, Jiwei Sun Dec 2014

High Frequency Devices And Circuit Modules For Biochemical Microsystems, Jiwei Sun

All Dissertations

This dissertation investigates high frequency devices and circuit modules for biochemical microsystems. These modules are designed towards replacing external bulky laboratory instruments and integrating with biochemical microsystems to generate and analyze signals in frequency and time domain. The first is a charge pump circuit with modified triple well diodes, which is used as an on-chip power supply. The second is an on-chip pulse generation circuit to generate high voltage short pulses. It includes a pulse-forming-line (PFL) based pulse generation circuit, a Marx generator and a Blumlein generator. The third is a six-port circuit based on four quadrature hybrids with 2.0~6.0 …

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Recent Advances In The Field Of Bionanotechnology: An Insight Into Optoelectric Bacteriorhodopsin, Quantum Dots, And Noble Metal Nanoclusters, Christopher Knoblauch, Mark Griep, Craig Friedrich Oct 2014

Recent Advances In The Field Of Bionanotechnology: An Insight Into Optoelectric Bacteriorhodopsin, Quantum Dots, And Noble Metal Nanoclusters, Christopher Knoblauch, Mark Griep, Craig Friedrich

Michigan Tech Publications, Part 1

Molecular sensors and molecular electronics are a major component of a recent research area known as bionanotechnology, which merges biology with nanotechnology. This new class of biosensors and bioelectronics has been a subject of intense research over the past decade and has found application in a wide variety of fields. The unique characteristics of these biomolecular transduction systems has been utilized in applications ranging from solar cells and single-electron transistors (SETs) to fluorescent sensors capable of sensitive and selective detection of a wide variety of targets, both organic and inorganic. This review will discuss three major systems in the area …

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Probabilistic Uncertainty Quantification And Experiment Design For Nonlinear Models: Applications In Systems Biology, Vu Cao Duy Thien Dinh Oct 2014

Probabilistic Uncertainty Quantification And Experiment Design For Nonlinear Models: Applications In Systems Biology, Vu Cao Duy Thien Dinh

Open Access Dissertations

Despite the ever-increasing interest in understanding biology at the system level, there are several factors that hinder studies and analyses of biological systems. First, unlike systems from other applied fields whose parameters can be effectively identified, biological systems are usually unidentifiable, even in the ideal case when all possible system outputs are known with high accuracy. Second, the presence of multivariate bifurcations often leads the system to behaviors that are completely different in nature. In such cases, system outputs (as function of parameters/inputs) are usually discontinuous or have sharp transitions across domains with different behaviors. Finally, models from systems biology …

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Synthesis And Evaluation Of Sensitizer Drug Photorelease Chemistry: Micro-Optic Method Applied To Singlet Oxygen Generation And Drug Delivery, Goutam Ghosh Oct 2014

Synthesis And Evaluation Of Sensitizer Drug Photorelease Chemistry: Micro-Optic Method Applied To Singlet Oxygen Generation And Drug Delivery, Goutam Ghosh

Dissertations, Theses, and Capstone Projects

This thesis summarizes a new micro-optic method for singlet oxygen generation and sensitizer drug delivery, which include i) synthesis and evaluation of a first generation device for drug delivery from native and fluorinated silica probe tips, ii) synthesis of PEG conjugated sensitizers to study phototoxicity in ovarian cancer cells, and iii) synthesis and evaluation of tris-PEGylated chlorin conjugated fluorinated silica for its future integration into the device to use as a 2nd generation device. A first generation micro-optic device was developed that works by sparging O2 gas and light generating cytotoxic singlet oxygen that cleaves the covalently attached drug (sensitizer) …

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Towards A Dynamic View Of Genetic Networks: A Kalman Filtering Framework For Recovering Temporally-Rewiring Stable Networks From Undersampled Data, Jehandad Khan Sep 2014

Towards A Dynamic View Of Genetic Networks: A Kalman Filtering Framework For Recovering Temporally-Rewiring Stable Networks From Undersampled Data, Jehandad Khan

Theses and Dissertations

It is widely accepted that cellular requirements and environmental conditions dictate the architecture of genetic regulatory networks. Nonetheless, the status quo in regulatory network modeling and analysis assumes an invariant network topology over time. We refocus on a dynamic perspective of genetic networks, one that can uncover substantial topological changes in network structure during biological processes such as developmental growth and cancer progression. We propose a novel outlook on the inference of time-varying genetic networks, from a limited number of noisy observations, by formulating the networks estimation as a target tracking problem. Assuming linear dynamics, we formulate a constrained Kalman …

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