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Electrohydrodynamic Atomization For Improved Macromolecular Drug Delivery, Michelle Zeles-Hahn

Electronic Theses and Dissertations

With advances in drug research, the use of biological therapeutics is becoming a reality. Unfortunately, methods for processing and delivering these fragile macromolecules often limit their therapeutic potential. For this dissertation, we explore the aerosolization of macromolecules by way of electrohydrodynamic atomization (EHDA) and how this method can be used to process and deliver therapeutics. EHDA employs a high voltage to break a column of liquid into drops. It was unknown if or how the residual charge left of the resulting droplets would affect lung cells. An in vitro experiment was conducted to spray aerosolized DNA, by way of EHDA, …

In-Vivo Testing Of Vertically Aligned Nanowire Implantable Titanium Electrodes In The Rattus Norvegicus Hippocampus, Lauren Kegley

Inquiry: The University of Arkansas Undergraduate Research Journal

Miniaturized multielectrode arrays are MEMS devices that have found use as neural prosthetics (Neuro-MEMS). As implants, they can interface with neurons as sensors or actuators and help compensate for loss of sensory input, motor control, or cognitive functions. The microelectrodes studied here were developed in-house. They have a vertically aligned gold nanowire array and are mounted on a sturdy titanium needle with a fine gauge. Hence, the bill of materials and design characteristics encourage their use as a neural probe. For this study, a probe was tested in vivo for signal acquisition in the hippocampus of a Rattus Norvegicus (Sprague …

Post-Project Assessment And Follow-Up Support For Community Managed Rural Water Systems In Panama , Ryu Suzuki

Dissertations, Master's Theses and Master's Reports - Open

The Environmental Health (EH) program of Peace Corps (PC) Panama and a non-governmental organization (NGO) Waterlines have been assisting rural communities in Panama gain access to improved water sources through the practice of community management (CM) model and participatory development. Unfortunately, there is little information available on how a water system is functioning once the construction is complete and the volunteer leaves the community. This is a concern when the recent literature suggests that most communities are not able to indefinitely maintain a rural water system (RWS) without some form of external assistance (Sara and Katz, 1997; Newman et al, …

Exploration Of The Role Of Serum Factors In Maintaining Bone Mass During Hibernation In Black Bears, Rachel Marie Bradford

Dissertations, Master's Theses and Master's Reports - Open

Disuse osteoporosis is a condition in which reduced mechanical loading (e.g. bed-rest, immobilization, or paralysis) results in unbalanced bone turnover. The American black bear is a unique, naturally occurring model for the prevention of disuse osteoporosis. Bears remain mostly inactive for up to half a year of hibernation annually, yet they do not lose bone mechanical strength or structural properties throughout hibernation. The long-term goal of this study is to determine the biological mechanism through which bears maintain bone during hibernation. This mechanism could pinpoint new signaling pathway targets for the development of drugs for osteoporosis prevention. In this study, …

Design And Development Of An Integrated Waste Management System For Huai Nam Khun, Thailand, Annie Duong

Industrial and Manufacturing Engineering

Background

A rural village in Thailand has always been limited in access to waste collection services and processing facilities. As a result, their behaviors of disposing waste in ineffective manners such as dumping and burning causes a variety of environmental impacts. In addition, the people dwelling in these areas are often unaware of the health risks associated to these types of engagements because of the inadequate education system. For a long time coming these circumstances remained untouched until a non-profit organization recently shed light to the need for a solution. An investigation, to develop a design solution in order to …

Environmental Savings Tracking System For Cisco Systems, Inc., Lauren J. Herrera

Industrial and Manufacturing Engineering

This paper illustrates the research, methodology and design used to create an environmental savings tracking system, also known as the Green Dashboard, for Cisco Systems Inc.’s Green Initiatives Group to quantify and capture an annualized summary of savings from projects initiated within its Customer Value Chain Management Organization. It articulately describes the

methodology behind the selection of metrics and the design of the process required to collect and track these quantitative direct savings. As well as describes the benefits associated with implementing this system, such as shifting Cisco towards more sustainable products and increased customer satisfaction and investor relations by …

Non-Planar Diamond Electrodes For Biomedical Neural Sensing And Stimulating, Jeffrey M. Halpern

Chemical Engineering

Abstract

Conductive diamond has exceptional chemical stability, making it an appealing candidate material for long-term medical device implants. This work describes the characterization, and fabrication of four diamond-based neural devices. A previously developed 30 µm diamond-disk in vitro needle electrode was characterized. The device could detect 1 nM concentration analytes in a flow cell using fast scan cyclic voltammetry, but the fabrication was not reproducible. A novel in vitro diamond device was developed with diamond selectively grown at the tip of a hollow quartz capillary, resulting in a 2-4 µm tip diameter. The diamond tip shape and size were geometrically …

Digester Selection And Operation For Heybeli Island, Spencer Fackler, Joseph Violetti

Industrial and Manufacturing Engineering

The Heybeli Digester project originated with Sema Alptekin in her personal research and was incorporated into a Senior Project opportunity at California Polytechnic State University, San Luis Obispo. The aim of this project was to select and design a plan for the use of a digester for the island of Heybeli, Turkey. The objective of this plan was to incorporate economic, social, and environmental justifications to evaluate if a digester would be a benefit to the community.

Systems Engineering analysis is a vital tool when working with complicated projects that include multiple systems interfacing with one another. Through the use …

Live Video Database Management Systems, Rui Peng

Electronic Theses and Dissertations

With the proliferation of inexpensive cameras and the availability of high-speed wired and wireless networks, networks of distributed cameras are becoming an enabling technology for a broad range of interdisciplinary applications in domains such as public safety and security, manufacturing, transportation, and healthcare. Today’s live video processing systems on networks of distributed cameras, however, are designed for specific classes of applications. To provide a generic query processing platform for applications of distributed camera networks, we designed and implemented a new class of general purpose database management systems, the live video database management system (LVDBMS). We view networked video cameras as …

Hot Water, Dry Streams: A Tale Of Two Trout, Jack R. Tuholske

All In-stream Flows Material

Norman Maclean’s timeless memoir A River Runs Through It begins with the reflection that in his household “there was no clear line between religion and fly fishing.”¹ That zeal remains today; nearly 30 million Americans call themselves fishermen.² Anglers devoted an aggregate of 517 million user-days pursuing their passion in 2006 and spent billions of dollars to support it.³ Fishing in the western United States holds a special place in fishing lore the world over and for good reason. From the great trout waters of Montana, to the salmon-laden rivers of the west coast, to the sparkling …

A Comparative Guide To The Western States' Public Trust Doctrines: Public Values, Private Rights, And The Evolution Toward An Ecological Public Trust, Robin Kundis Craig

All In-stream Flows Material

In the arid West, balancing private needs for fresh water to consume against the public values-recreational, aesthetic, and ecological-served by leaving fresh water in situ has tended to favor the private use side. Evidence of this result is both massive and minor, ranging from California's multi-billion-dollar water transportation system,¹ to the routine de-watering of the Colorado River so that little to no water reaches the Sea of Cortez,² to water-related Endangered Species Act lawsuits in dozens of watersheds. ³

Ablation Of Chronic Total Occlusions Using Kilohertz-Frequency Mechanical Vibrations In Minimally Invasive Angioplasty Procedures., Garrett Mcguinness, Mark Wylie, Graham Gavin

Articles

Certain minimally invasive cardiology procedures, such as balloon angioplasty and stent implantation, critically require that the site of an arterial blockage be crossed by an intraluminal guidewire. Plaques resulting in near or totally occluded arteries are known as chronic total occlusions (CTOs), and crossing them with conventional guidewires is a significant challenge. Among the most promising proposed solutions is the delivery of high power, low frequency ultrasonic vibrations to the occlusion site via an intraluminal wire waveguide. The vibrating distal-tip of the ultrasound wire waveguide is used to transmit energy to the surrounding plaques, tissues and fluids in order to …

Santa Clara Magazine, Volume 52 Number 1, Summer 2010, Santa Clara University

Santa Clara Magazine

16 - COURAGE IN THE FACE A photoessay from Haiti by Michael Lattimore. A journey to document humanitarian work by doctors in the wake of the January quake.

20 - PILGRIMAGE By Martha Stortz. Walking the Camino to Santiago de Compostela, and learning a few things along the way: about big questions, saints, direction, and feet.

28 - THE HISTORIANS An interview by Ron Hansen M.A '95. Between them, historians George Giacomini '56 and Tim O'Keefe can claim nearly a century of educating Santa Clara students. This year, both close the books on teaching in the classroom. But first they …

Status And Trend Of Cottonwood Forests Along The Missouri River, Mark D. Dixon, W. Carter Johnson, Michael L. Scott, Daniel Bowen

United States Army Corps of Engineers, Omaha District

From 2007-2009, we studied the current (2006) and historic (1892, mid-1950s) extent, current age distribution, and plant species composition of plains cottonwood (Populus deltoides) and non-cottonwood riparian stands along eight study segments of the Missouri River between Fort Benton, Montana and Kansas City Missouri, covering 930 river miles (1500 km) or over 1/3 of the river's length. These segments included all of the unchannelized and unimpounded segments below Fort Benton, as well as portions of two impounded and one channelized segment. Based on GIS analyses of historic maps and aerial photography, the combined area of forests, woodlands, and …

A Case Study: Teaching Engineering Concepts In Science, David R. Stricker

Reports of Center Studies

This study was conducted to describe a teacher developed high school engineering course, to identify teaching strategies used in the process of delivering math and science literacy through this course, to identify challenges and constraints that occurred during its development and delivery, and to describe the strategies that were used to overcome those obstacles.

A case study was conducted using semi-structured interviews with the engineering instructor at Benilde-St. Margaret's in St. Louis Park, Minnesota. In addition, the researcher conducted classroom observations and reviewed instructional materials, teacher lesson plans, and teacher journals.

Themes that developed regarding the strategies used to deliver …

Design, Development And Biomechanical Analysis Of Scaffolds For Augmentation Of Rotator Cuff Repairs, Amit Aurora

ETD Archive

Rotator cuff tears are a source of debilitating pain that commonly affects more than 40 of our aging population. Despite advances in surgical treatment, the failure rate of rotator cuff repairs is as high as 20-90 . Extracellular matrix (ECM) derived scaffolds have recently been investigated as augmentation devices for rotator cuff repairs, but none has yet demonstrated both the appropriate biological and mechanical properties for mitigating re-tears and enhancing healing. This dissertation proposes to engineer the mechanical properties of allograft fascia lata in a manner that will allow its use as an augmentation device for rotator cuff repairs. This …

Surface Microtopography Modulation Of Biomaterials For Bone Tissue Engineering Applications, Eun Jung Kim

ETD Archive

The ultimate goal of this project is to develop a biodegradable and implantable scaffold with precise surface topographies that can provide osteoconductive stimuli to connective tissue progenitor cells (CTPs), and subsequently, enhance bone regeneration applications without the complications of autogenous cancellous bone grafts. This dissertation presents the modification of surface microtextures to provide osteoconductive stimuli to CTPs for bone regeneration applications. First, the effect of surface topography on cell proliferation and osteogenic differentiation was validated through experiments using surface post microtextures and CTPs. Post microtextures accelerated CTP growth behaviors compared to smooth polydimethylsiloxane (PDMS) and standard cell culture dishes. Second, …

Developing A Protocol For An Environmentally Preferable Purchasing Plus (Epp+) Program At Nasa Glenn Research Center, Amarin Kongtawelert

ETD Archive

EPP+ or Environmentally Preferable Purchasing Plus is a Environmentally Preferable Purchasing program with incorporated a unique Life Cycle Assessment (LCA) scoring system which implemented at NASA, Glenn Research Center. A protocol has been developed to aid in selecting among products based on principles of Environmentally Preferable Purchasing (EPP) and Life Cycle Assessment (LCA). The protocol has been developed for use at NASA Glenn Research Center (GRC) but is intended to benefit anyone wishing to make sustainable purchasing decision. The protocol was designed as a web-based application which evaluates the life cycle factors, affirmative procurements, price, and performance of products that …

Cell-Taught Gene Therapy For The Preservation And Regeneration Of Cardiac Tissue Following Chronic Heart Failure, Srividya Sundararaman

ETD Archive

Heart failure is the primary cause of mortality and morbidity in the Western world. Although cell therapy has demonstrated improvement in cardiac function, these benefits are being attributed to the activation of paracrine factors, rather than the differentiation and integration of the transplanted cells into the host tissue. Based on this knowledge the focus of this thesis work was to deliver paracrine factors, and evaluate its effect on cardiac function. Gene therapy has evolved as a promising option to deliver pro-angiogenic proteins to infarct zones, thus providing cardiac benefit. This study has identified a gene design without the use of …

Automatic Utility Meter Reading, Kaicheng Xie

ETD Archive

This thesis discusses research in the automation of reading a utility meter. The hardware required is a wireless camera pointed at the meter to be read. This meter can be a water, electricity or gas meter. After the wireless camera collects the image from the utility meter it transmits the image to a computer through a receiver. The computer analyzes the image with pattern recognition technology to obtain the numbers. We use image processing techniques in this thesis, including image preprocessing, image segmentation (Otsu threshold method), and number recognition. Finally we create a graphical user interface using Visual C#®

A Theoretical Model Of The Effect Of Bone Defects On Anterior Shoulder Instability: A Finite Element Approach, Piyush Walia

ETD Archive

Presence of either a Hill Sachs or a Bony Bankart lesion has been indicated as a possible cause of subluxation and anterior shoulder dislocation. Previous studies have investigated only effects of these isolated lesions on the glenohumeral instability of the shoulder. The purpose of this thesis was to investigate the effect of both Bony Bankart lesion and Hill-Sachs lesion in the glenohumeral joint on the stability of the shoulder. We hypothesize that as the size of the lesion increases, the glenohumeral joint's stability will decrease. We further hypothesize that the presence of both defects together will reduce the glenohumeral joint's …

Study Of Strawberry Flavored Milk Under Pulsed Electric Field Processing, Daniela Bermúdez-Aguirre, Jaime A. Yáñez, C. Patrick Dunne, Neal M. Davies, Gustavo V. Barbosa-Cánovas

United States Army: Publications

Few studies exist on flavored milk processed by pulsed electric fields (PEF). The main concern is product stability. This study aimed to analyze the degradation of coloring agent Allura Red in strawberry milk under PEF. Four systems were tested containing Allura Red: two commercial milks and two model systems. PEF conditions were 40 kV/cm, 48 pulses (2.5 μs), and 55 °C; coloring agent was quantified via RP-HPLC. After processing, only minor changes were observed in color, Allura Red concentration, and pH. During storage (32 d) at refrigerated conditions (4 °C) commercial samples maintained pH above 6. Model systems dropped below …

Acute Caffeine Consumption Enhances The Executive Control Of Visual Attention In Habitual Consumers, Tad T. Brunyé, Caroline R. Mahoney, Harris R. Lieberman, Grace E. Giles, Holly A. Taylor

United States Army: Publications

Recent work suggests that a dose of 200–400 mg caffeine can enhance both vigilance and the executive control of visual attention in individuals with low caffeine consumption profiles. The present study seeks to determine whether individuals with relatively high caffeine consumption profiles would show similar advantages. To this end, we examined the effects of four caffeine doses (0 mg, 100 mg, 200 mg, 400 mg) on low- and high-level visual attention in individuals with high consumption profiles (n = 36), in a double-blind study using a repeated measures design. Results from the Attention Network Test indicated that caffeine enhanced both …

Genomics And Proteomics In Chemical Warfare Agent Research: Recent Studies And Future Applications, Patrick A. Everly, James F. Dillman

United States Army: Publications

Medical research on the effects of chemical warfare agents (CWAs) has been ongoing for nearly 100 years, yet these agents continue to pose a serious threat to deployed military forces and civilian populations. CWAs are extremely toxic, relatively inexpensive, and easy to produce, making them a legitimate weapon of choice for terrorist organizations. While the mechanisms of action for manyCWAshave been known for years, questions about their molecular effects following acute and chronic exposure remain largely unanswered. Global approaches that can pinpoint which cellular pathways are altered in response to CWAs and characterize long-term toxicity have not been widely used. …

The Effects Of Bleedarrest, Celox, And Traumadex On Hemorrhage Control In A Porcine Model, Brian Gegel, James Burgert, Brian Cooley, Jacob Macgregor, Jules Myers, Sean Calder, Ralph Luellen, Michael Loughren, Don Johnson

United States Army: Publications

Background

Hemorrhage is the second leading cause of death in civilian trauma and the leading cause of preventable death in military trauma. The purpose
of this study was to examine the effectiveness of three hemostatic agents: BleedArrest, TraumaDex, and Celox.

Materials and Methods

This was a prospective, experimental study using male Yorkshire swine. The pigs (n = 5 per group) were randomly assigned to one of the following: BleedArrest, TraumaDex, Celox, or control. To simulate a trauma injury, the investigators generated a complex groin injury with transection of the femoral artery and vein in all pigs. After 1 min of …

Effects Of The Heet Garment In The Prevention Of Hypothermia In A Porcine Model, Don Johnson, Brian Gegel, James Burgert, Geoffrey W, Duncklee, Ricci R. Robinson, Eric J. Lewis, Paul M. Crum, William Kuhns, Daniel Moore, Scott O'Brien, Joel Elliott, Jason Washington, John Boyle, Dale Seigler

United States Army: Publications

Background

Hypothermia is a common battlefield trauma occurrence. This study compared the effectiveness of the hypothermia, environmental, exposure, and trauma (HEET) garment (Trident Industries, Beaufort, SC) with and without thermal inserts with a control group of two wool blankets in the prevention of hypothermia in a treated hypovolemic porcine model.

Materials and methods

Five female swine (Sus scrofa-Yorkshire cross) were assigned to each of three
groups: HEET with thermal inserts (n=5); HEET without thermal inserts (n=5); or control (n=5). After the animals were anesthetized and stabilized for 30min, the swine were hemorrhaged to a mean arterial pressure (MAP) of 30mm …

Inflammatory Effects Of Inhaled Sulfur Mustard In Rat Lung, Rama Malaviya, Vasanthi R. Sunil, Jessica Cervelli, Dana R. Anderson, Wesley W. Holmes, Michele L. Conti, Ronald E. Gordon, Jeffrey D. Laskin, Debra L. Laskin

United States Army: Publications

Inhalation of sulfur mustard (SM), a bifunctional alkylating agent that causes severe lung damage, is a significant threat to both military and civilian populations. The mechanisms mediating its cytotoxic effects are unknown and were investigated in the present studies. Male rats Crl:CD(SD) were anesthetized, and then intratracheally intubated and exposed to 0.7–1.4 mg/kg SM by vapor inhalation. Animals were euthanized 6, 24, 48 h or 7 days post-exposure and bronchoalveolar lavage fluid (BAL) and lung tissue collected. Exposure of rats to SM resulted in rapid pulmonary toxicity, including focal ulceration and detachment of the trachea and bronchial epithelia from underlying …

Inhibition Of Hiv-1 Infection Of Peripheral Blood Mononuclear Cells By A Monoclonal Antibody That Binds To Phosphoinositides And Induces Secretion Of Β-Chemokines, Gary R. Matyas, Lindsay Wieczorek, Divya Bensal, Agnes-Laurence Chenine, Eric Saunders-Buell, Sodsai Tovanabutra, Jerome H. Kim, Victoria Polonis, Carl R. Alving

United States Army: Publications

A murine IgG mAb, WR321, selected for the ability to bind to phosphatidylinositol-4-phosphate and phosphatidylinositol-4, 5-bisphosphate, but an inability to bind to any of 17 other lipids, including phosphatidylinositol, was examined as a probe for studying interactions of HIV-1 with primary human peripheral blood mononuclear cells. The WR321 mAb broadly neutralized CCR5-tropic strains of HIV-1 to prevent infection of the cells. The mAb also exhibited direct interaction with cells in the culture, resulting in secretion of chemokines that interfered with the interaction of HIV-1 virions with CCR5, the coreceptor for HIV-1 on the susceptible cells, leading to inhibition of infection …

Current Us Military Operations And Implications For Military Surgical Training, Joshua A. Tyler, Kevin S. Clive, Christopher E. White, Alec C. Beekley, Lorne H. Blackbourne

United States Army: Publications

BACKGROUND:

Since 2001, US military surgeons have deployed frequently, with many surgeons deploying within 1 year of graduating residency. The purpose of this study was to evaluate readiness of recent graduates to manage combat-related injuries and to make recommendations for improvements in training military surgeons.

STUDY DESIGN:

We reviewed casualties treated at the 31^st Combat Support Hospital in Baghdad from December 2003 to November 2004.We identified 3,426 wounded patients; of these, 2,648 (77.3%) required an operative procedure. There were 2,788 patients (81.4%) who sustained penetrating injuries. The most common procedures performed were debridement of wounds (39%), skeletal fixation (14.7%), …

Design Of A Polysaccharide Derived Tissue Engineered Vascular Graft, Irina Robu

Wayne State University Dissertations

DESIGN OF A POLYSACCHARIDE DERIVED TISSUE ENGINEERED VASCULAR GRAFT

By

Irina Robu

November 2010

Advisor: Dr. Howard Matthew

Major: Chemical Engineering

Degree: Doctor of Philosophy

As the most frequent congenital anomaly and the leading cause of death, congenital heart disease is an attractive target for tissue engineering research. The challenging target to engineer a native blood vessel stems from the need to find substitutes for pediatric patients born with congenital defects. The tissue engineering graft functions to bring various cells of the vasculature in close proximity to one another to enable intercellular signaling which facilitates cell adhesion, migration and differentiation. …