Digital Commons Network^™

Evaluating Chemical Toxicity: A Novel Behavioral Assay Using Daphnia, Maya A. Zein

Wayne State University Dissertations

Pharmaceuticals, and personal care products (PPCPs), and other emerging contaminants, such as pesticides, are increasingly being detected in the environment. Important sources of these contaminants are waste water treatment plants and agriculture. Many of these contaminants are biologically active at low concentrations, and may impair physiological processes in exposed organisms, alter reproductive, endocrine or immune system function, and ultimately affect fitness and survival. These chemicals are often found in the environment as complex mixtures, and this complicates their evaluation of their toxicity. There is a need for high-throughput assays to rapidly assess the toxicity of these emerging contaminants. A behavioral …

Post-Project Assessment Of Pit Latrines In Rural Panama, Jacob B. Midkiff

Dissertations, Master's Theses and Master's Reports - Open

In Panama, one of the Environmental Health (EH) Sector’s primary goals is to improve the health of rural Panamanians by helping them to adopt behaviors and practices that improve access to and use of sanitation systems. In complying with this goal, the EH sector has used participatory development models to improve hygiene and increase access to latrines through volunteer managed latrine construction projects. Unfortunately, there is little understanding of the long term sustainability of these interventions after the volunteers have completed their service. With the Peace Corps adapting their Monitoring, Reporting, and Evaluation procedures, it is appropriate to evaluate the …

New Highway Accident Location Manual For Missouri, Carlos Sun Ph.D., P.E., Jd, Praveen Edara Ph.D., P.E., Ptoe, Henry Brown M.S., P.E.

Mid-America Transportation Center: Final Reports and Technical Briefs

The Missouri HAL manual is used to identify, analyze, and correct high crash locations, and has not been updated since 1999. This new edition brings the manual up to date, while incorporating the methodology of the national Highway Safety Manual (HSM). This 4th edition represents a complete re-working of all existing chapters of the manual. The changes are both stylistic and substantive. A contemporary book-style stylesheet was used to improve the appearance of figures, tables, headings, and labels. Even the title of the manual was changed from HAL (Identification, Analysis, and Correction of High-Crash Locations) to S-HAL (Safety Handbook for …

Nonlinear Granger Causality And Its Application In Decoding Of Human Reaching Intentions, Mengting Liu

Doctoral Dissertations

Multi-electrode recording is a key technology that allows the brain mechanisms of decision making, cognition, and their breakdown in diseases to be studied from a network perspective. As the hypotheses concerning the role of neural interactions in cognitive paradigms become increasingly more elaborate, the ability to evaluate the direction of neural interactions in neural networks holds the key to distinguishing their functional significance.

Granger Causality (GC) is used to detect the directional influence of signals between multiple locations. To extract the nonlinear directional flow, GC was completed through a nonlinear predictive approach using radial basis functions (RBF). Furthermore, to obtain …

Design And Analysis Of A Compression Molded Carbon Composite Wheel Center, Vinoth Kumar Dhananjayan

Mechanical and Aerospace Engineering Theses - Archive

Importance of closed mold and short fibers in the manufacture of carbon composite parts was realized based on a study of existing manufacturing processes for thermoset resins as these can improve production volumes, cost reduction of the part, production of near net shape parts eliminating secondary operations and production of parts with near isotropic properties due to random fiber orientation. Compression molding process is the most preferred process as this addresses the problems of fiber damage effectively because of the minimal flow in the material. A brief study was done on the compression molded carbon composite parts in the industry. …

Establishment And Characterization Of An Animal Model Of Distraction Spinal Cord Injury, Jennifer Elisabeth Stearns Bell

Bioengineering Dissertations - Archive

Scoliosis corrective surgery requires the application of multidirectional stress forces to the spinal cord, including those of distraction, in addition to the application of fixation rods for correction of the curved spine deformity. If excessive, spine distraction may result in the development of new neurologic deficits, some as severe as permanent paralysis. Intraoperative monitoring is used to alert the surgeon to possible complications; however, the complex nature of spinal cord injury (SCI) involves a primary mechanical insult to the tissue and/or vasculature that may go undetected by monitoring. This is followed by the activation of widespread secondary injury mechanisms. The …

Diffuse Optical Tomography To Study The Ischemic Stroke In Rat Models, Zi-Jing Lin

Bioengineering Dissertations - Archive

Stroke, including ischemic stroke and hemorrhagic stroke, is the major leading cause of death in the United States. More than 80 percent of stroke patients are diagnosed with ischemic stroke, which usually results from blockage of artery in the brain by thrombosis or arterial embolism. Animal Models, which permit powerful genetic and molecular approaches, provide an essential tool to study and understand the basic processes and potential therapeutic interventions for this disease. Significant efforts have been made to closely mimic the changes that occur in humans during and after stroke using animal models. Recent development on near infrared (NIR) diffuse …

Noninvasive Assessment Of Tumor Hypoxia Using Oxygen Sensitive Mri In Prostate Tumor Models, Rashmi Reddy Pabhathi Reddy

Bioengineering Theses - Archive

A variety of solid tumors reveal oxygen deficiency as a result of rapid growth and insufficient tumor angiogenesis. Hypoxia in solid tumors appears to speed up malignant progression and metastatic potential of primary tumors thus leading to resistance against anticancer drugs and decreased response to radiotherapy.Of various in vivo non-invasive techniques to assess tumor hypoxia, oxygen-sensitive MRI (BOLD and TOLD) is an attractive and reliable option. Blood Oxygen Level Dependent (BOLD) and Tissue Oxygen Level Dependent (TOLD) MRI offer potential insight into tumor hypoxia. BOLD (Blood Oxygen Level Dependant) contrast MRI which measures R2* (1/T2*) is sensitive to endogenous paramagnetic …

Development Of Non-Invasive Methods For Imaging Inflammatory Response, Yi-Ting Tsai

Bioengineering Dissertations - Archive

Persistent inflammatory cell reactions are responsible for the pathogenesis of a variety of inflammatory diseases. Although many non-invasive imaging methods have been used in the clinics to monitor the extent of inflammatory responses, almost all of them are based on indirect detection of inflammation-associated anatomical or structure changes of tissues and organs. To fill the gap, our laboratory has focused research efforts on detecting different processes of inflammatory responses. Our results can be summarized in the sequence of inflammatory responses - edema formation, inflammatory cell recruitment, inflammatory product release, and cell death. Shortly after medical device implantation or injury, edema …

Occurrence Of Antimicrobials And Antimicrobial Resistance Genes In Beef Cattle Storage Ponds And Swine Treatment Lagoons, Yuping Zhang, Chiqian Zhang, David B. Parker, Daniel D. Snow, Zhi Zhou, Xu Li

Nebraska Water Center: Faculty Publications

Livestock manure treatment and storage structures are potential environmental sources of antimicrobials and antimicrobial resistance genes (ARGs). In this study, the occurrence of antimicrobials and ARGs was investigated in the water and the sludge compartments of beef cattle storage ponds and swine lagoons. Analysis was focused on two families of antimicrobials (sulfonamide and tetracycline) and the corresponding ARGs (sul1, sul2, tetO, tetQ and tetX). Results showed that the pseudo-partitioning coefficients of tetracyclines were higher than those of sulfonamides, suggesting different distributions of these two classes of antimicrobials between water and sludge. The ARGs tested were …

Runoff Nutrient Transport As Affected By Land Application Method, Swine Growth Stage, And Runoff Rate, J. E. Gilley, Shannon L. Bartelt-Hunt, S. J. Lamb, X. Li, D. B. Marx, D. D. Snow, D. B. Parker, B. L. Woodbury

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study was conducted to measure the effects of slurry application method, swine growth stage, and flow rate on runoff nutrient transport. Swine slurry was obtained from production units containing grower pigs, finisher pigs, or sows and gilts. The swine slurry was applied using broadcast, disk, or injection methods at a rate required to meet annual nitrogen requirements for corn. Three 30 min simulated rainfall events, separated by 24 h intervals, were applied to the experimental plots at an intensity of 70 mm h^-1. Inflow was applied at the top of each plot in four successive increments after …

Use Of A Surrogate To Evaluate The Impact Of Tillage On The Transport Of Steroid Hormones From Manure-Amended Agricultural Fields, Sagor Biswas, William Kranz, Charles A. Shapiro, Mitiku Mamo, Shannon L. Bartelt-Hunt, Daniel D. Snow, David P. Shelton, David D. Tarkalson, Terry L. Mader, Simon J. Van Donk, Tian C. Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef feedlot manure distributed to row crop production areas is a potential surface water contaminant source of the steroid hormones commonly used in beef cattle production. This article reports on research conducted at the University of Nebraska Haskell Agricultural Laboratory near Concord, Nebraska, in July 2009. Manure, collected from beef feedlot pens, was stockpiled for ten months prior to application to a row crop field. Previous research identified that the detection frequency of steroid hormones in beef manure varies greatly. Thus, a surrogate (17α-ethynylestradiol, EE2) was applied at a rate of 75 g ha^-1 to ensure detectable concentrations in …

Transport Of Steroid Hormones In The Vadose Zone After Land Application Of Beef Cattle Manure, Simon Van Donk, Sagor Biswas, William L. Kranz, Daniel D. Snow, Shannon L. Bartelt-Hunt, Terry L. Mader, Charles A. Shapiro, David P. Shelton, David D. Tarkalson, Tian .. Zhang, Steve Ensley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Few studies have been conducted to investigate the potential contamination of groundwater from manureborne steroid hormones. The objective of this study was to monitor leaching of steroid hormones at the bottom of 2.4 m deep percolation lysimeters installed in plots treated with beef cattle manure. Soil samples were also collected from surrounding field plots. Treatments consisted of application of manure from two treatment methods (stockpiling and composting) or no manure application. Laboratory analyses of manure, soil, and leachate samples used liquid chromatography and tandem mass spectrometry to identify 17 steroid hormones and metabolites. Progesterone, estrone, β-zearalenol, and 4-androstenedione were detected …

Robust Subspace Estimation Using Low-Rank Optimization. Theory And Applications In Scene Reconstruction, Video Denoising, And Activity Recognition., Omar Oreifej

Electronic Theses and Dissertations

In this dissertation, we discuss the problem of robust linear subspace estimation using low-rank optimization and propose three formulations of it. We demonstrate how these formulations can be used to solve fundamental computer vision problems, and provide superior performance in terms of accuracy and running time. Consider a set of observations extracted from images (such as pixel gray values, local features, trajectories . . . etc). If the assumption that these observations are drawn from a liner subspace (or can be linearly approximated) is valid, then the goal is to represent each observation as a linear combination of a compact …

Influence Of Task-Role Mental Models On Human Interpretation Of Robot Motion Behavior, Scott Ososky

Electronic Theses and Dissertations

The transition in robotics from tools to teammates has begun. However, the benefit autonomous robots provide will be diminished if human teammates misinterpret robot behaviors. Applying mental model theory as the organizing framework for human understanding of robots, the current empirical study examined the influence of task-role mental models of robots on the interpretation of robot motion behaviors, and the resulting impact on subjective ratings of robots. Observers (N = 120) were exposed to robot behaviors that were either congruent or incongruent with their task-role mental model, by experimental manipulation of preparatory robot task-role information to influence mental models (i.e., …

Suction Detection And Feedback Control For The Rotary Left Ventricular Assist Device, Yu Wang

Electronic Theses and Dissertations

The Left Ventricular Assist Device (LVAD) is a rotary mechanical pump that is implanted in patients with congestive heart failure to help the left ventricle in pumping blood in the circulatory system. The rotary type pumps are controlled by varying the pump motor current to adjust the amount of blood flowing through the LVAD. One important challenge in using such a device is the desire to provide the patient with as close to a normal lifestyle as possible until a donor heart becomes available. The development of an appropriate feedback controller that is capable of automatically adjusting the pump current …

Geothermal Injection Well Monitoring, Timothy Rempher

Electrical Engineering

People depend on electricity all over the world. They depend on it for anything from drying their hair to global communications to heating up food. Many methods of power generation exist, each using a different fuel such as nuclear power, natural gas, coal, wind or the sun. This project focuses on a power plant which uses the naturally hot water in the ground to generate electricity. This process of generating power is called “Geothermal.” This project consists of two parts. The first and major part is the design logic of a PLC program that monitors the flow rate of an …

Biomarkers For Brain Disorders Electrochemically Detected By Broderick Probe Microelectrodes/Biosensors, Waqas Saleem, Patricia A. Broderick

Publications and Research

Here, we present results from two independent studies carried out using Neuromolecular Imaging (NMI) with miniature BRODERICK PROBE® biosensors. In the first study, we imaged neurotransmitters and neurochemicals in human epilepsy patients intraoperatively during early and late neurodegeneration. In the second study, we imaged neurotransmitters and neurochemicals in an experimental murine model using animals with and without neurodegeneration caused by Parkinson’s disease (PD). We compared our results derived from animals with lesioned group (PD) with non-lesioned group (non-PD), using the same in vivo NMI paradigm. NMI biotechnology enabled neurotransmitters, neuropeptides and neurochemical imaging of dopamine (DA), serotonin (5-HT), homovanillic acid …

Ozone And Gac Treatment Of A Central Florida Groundwater For Sulfide And Disinfectant By-Product Control, Tara Lamoureux

Electronic Theses and Dissertations

This study evaluated the combination of ozone and granular activated carbon (GAC) treatment for the removal of sulfide and disinfection byproduct (DBP) precursors in drinking water at the pilot-scale. The research conducted was performed at the Auxiliary (Aux) and Main Water Treatment Plants (WTPs) in Sanford, Florida. Both WTPs rely upon groundwater sources that contain total sulfide ranging from 0.02 to 2.35 mg/L and total organic carbon (TOC) ranging from 0.61 to 2.20 mg/L. The Aux WTP’s raw water contains, on average, 88% more sulfide and 24% more TOC than the Main WTP. Haloacetic acids (HAA5) and total trihalomethanes (TTHMs) …

Time And Space Efficient Techniques For Facial Recognition, Waleed Alrasheed

Electronic Theses and Dissertations

In recent years, there has been an increasing interest in face recognition. As a result, many new facial recognition techniques have been introduced. Recent developments in the field of face recognition have led to an increase in the number of available face recognition commercial products. However, Face recognition techniques are currently constrained by three main factors: recognition accuracy, computational complexity, and storage requirements. The problem is that most of the current face recognition techniques succeed in improving one or two of these factors at the expense of the others. In this dissertation, four novel face recognition techniques that improve the …

Use Of Focused Ultrasound For Transcranial Sonothrombolysis, Golnaz Ahadi

Wayne State University Dissertations

Worldwide, stroke is the second most common cause of death. Ischemic stroke remains accountable for the majority of the 20 million devastating stroke events occurring globally each year. Use of transcranial focused ultrasound (FUS) for sonothrombolysis is a new research field for ischemic stroke treatment. If shown to be effective, FUS sonothrombolysis could become a widely available treatment option for the majority of the stroke sufferers that do not qualify or do not have access to current thrombolytic treatment options, such as tPA or neurointerventional methods.

The current study was an in vitro transcranial FUS headsystem investigation for the potential …

Development Of A Two-Color Optical Diagnostic For The Determination Of Engine In-Cylinder Soot Temperature And Volume Fraction Evolution With A Flame-Calibrated Emissivity Model, Kan Zha

Wayne State University Dissertations

A fundamental understanding of advanced compression ignition combustion is requisite to meet the simultaneous challenges of stringent fuel efficiency and emission standards. Single zone simulation shows that nitrogen oxide (NOx) production occurs in a high temperature region and soot production in a high equivalence ratio region within a specific temperature window. Combustion temperature, therefore, is a crucial variable that determines soot and NOx emissions under various combustion modes, and it is thus very important to have the capability to quantify this parameter in-cylinder. Optical diagnostic techniques such as -- Rayleigh scattering, filtered Raman scattering (Doppler), Raman scattering, coherent anti-stokes Raman …

Diagnosis Of Systemic Inflammation Using Transendothelial Electrical Resistance And Low-Temperature Co-Fired Ceramic Materials, William L. Mercke

Theses and Dissertations--Chemical and Materials Engineering

Systemic inflammation involves a complex array of cytokines that can result in organ dysfunction. Mortality remains high despite the vast amount of research conducted to find an effective biomarker. The cause of systemic inflammation can be broad and non-specific; therefore, this research investigates using transendothelial electrical resistance (TEER) measurements to better define systemic inflammatory response syndrome (SIRS)/sepsis within a patient. Results show a difference in TEER measurements between healthy individuals and SIRS-rated patients. This research also displays correlations between TEER measurements and biomarkers currently studied with systemic inflammation (tumor necrosis factor-α, C- reactive protein, procalcitonin). Furthermore, this research also presents …

Effects Of Coal Combustion By-Product–Encapsulated Ammonium Nitrate On Wheat Yield And Uptake Of Nitrogen And Metals, Eton E. Codling

Coal Combustion and Gasification Products Journal Archive

Ammonium nitrate (AN) is an essential plant nutrient for plant growth. Ammonium nitrate is also an ingredient in explosives. Research is being conducted on reducing the explosiveness of AN by encapsulating it with the use of coal by-products. The objective of this greenhouse pot study was to determine the effects of class C and F fly ash and flue gas desulfurization-gypsum-encapsulated AN on biomass, grain, and straw yield and nitrogen and metals uptake by wheat. Encapsulated AN was mixed with a low-nitrogen soil at 56 and 112 kg N ha^-1^, as well as unencapsulated AN as a control, and planted …

Designing Contour Weep Berms To Reduce Agricultural Nonpoint Source Pollution, Richard C. Warner, Carmen T. Agouridis, Ross L. Guffey

Biosystems and Agricultural Engineering Faculty Publications

Nonpoint source pollution (NPS) of surface waters is a significant issue in agricultural lands, and best management practices (BMPs) are often used to reduce these impacts. Since the effectiveness of a BMP depends on a large number of widely varying factors, it is important to continue to develop BMPs in order to provide designers with more tools to use to maximize NPS removal. The contour weep berm is a new structural BMP constructed out of earth and subsequently vegetated. It is a linear BMP that is used in combination with a down-gradient vegetated filter strip or forested riparian buffer. Preliminary …

Using Power-Law Properties Of Social Groups For Cloud Defense And Community Detection, Justin L. Rice

Doctoral Dissertations

The power-law distribution can be used to describe various aspects of social group behavior. For mussels, sociobiological research has shown that the Lévy walk best describes their self-organizing movement strategy. A mussel's step length is drawn from a power-law distribution, and its direction is drawn from a uniform distribution. In the area of social networks, theories such as preferential attachment seek to explain why the degree distribution tends to be scale-free. The aim of this dissertation is to glean insight from these works to help solve problems in two domains: cloud computing systems and community detection.

Privacy and security are …

Engineering Microenvironments To Modulate Calcium Information Processing In Neuronal Cells, Kinsey Cotton Kelly

Doctoral Dissertations

Tissue engineered microenvironments were constructed to test the effects glial cells have on calcium information processing, and to mimic conditions in vivo for tumor invasion and residual cancer after resection of tumor. Submaximal, nM, glutamate (GLU) stimuli were applied to the engineered environments, and the resulting calcium dynamic behavior of neuronal cells was measured to help predict and interpret chaotic systems in the experimental realm. Calcium is a key signaling ion which signals through the N-methyl-D-aspartate (NMDA) glutamate receptor on the neuronal membrane. GLU binding to the NMDA receptor (NMDAR) causes a large and dynamic increase in neuronal intracellular calcium. …

Scanner: An Efficient And Accurate Trimming Tool For Illumina Next Generation Sequencing Reads, Xiang Zhou

All Computer Science and Engineering Research

Recent advances in High-Throughput Sequencing (HTS) technology have greatly facilitated the researches in bioinformatics field. With the ultra-high sequencing speed and improved base-calling accuracy, Illumina Genome Analyzer is currently the most widely used platform in the field. To use the raw reads generated from the sequencing machine, the 3’ adapter sequence attached to the real read in the process of ligation needs to be correctly trimmed. This is often done by some inhouse scripts or different packages with various parameters. They either use the Smith-Waterman algorithm or search for an exact match of the 3’ adapter sequence. In this report, …

The Relationship Between Traffic Signals And Pedestrian, Bicyclist And Transit User Exposure In Urban Areas, Courtney Natasha Slavin

Dissertations and Theses

Improving the efficiency of traffic operations along arterials is currently a priority for many agencies as their roadway infrastructure is built out to the largest possible capacity within the urban environment. Many advanced systems are being implemented to optimize traffic signal timing. Air quality is typically not a consideration when these decisions are made. The relationship between exposure to air pollution and traffic signal timing has not been fully explored by other researchers. This study is the first research effort to combine detailed traffic signal timing data (at 5 second intervals) and air pollutant exposure data. Results show that longer …

Optimization Of Bioprinting Process Using Design Of Experiments (Doe) And Taguchi Method, Maria Veronica Gonzalez

Open Access Theses & Dissertations

At this time there is a need for new fabrication techniques for engineering complex biological structures related to regenerative medicine, in vitro tissue analysis, and pharmaceutical testing with the purpose of placing specific cells in specific target locations. Right now, there is no enough conventional cell seeding methods that can achieve it. In order to achieve it, bio-fabrication, which is a delivery mechanism capable of placing biomaterials, cells, and bioactive macromolecules in specific target locations, comes to play an important role in this area. Among different approaches, bioprinting is one of the most useful processes that use drop-on-demand delivery mechanisms …