Digital Commons Network^™

Kinetics Of Lipid Extraction From Microalgae, Brian Mcconnell

Honors Theses and Capstones

No abstract provided.

Mechanics Of Blast Loading On Post-Mortem Human And Surrogate Heads In The Study Of Traumatic Brain Injury (Tbi) Using Experimental And Computational Approaches, Shailesh Govind Ganpule

Department of Engineering Mechanics: Dissertations, Theses, and Student Research

Blast induced neurotrauma (BINT) has been designated as the “signature injury” to warfighters in the recent military conflicts. The occurrence of traumatic brain injury (TBI) in blasts is controversial in the medical and scientific communities because the manifesting symptoms occur without visible injuries. Whether the primary blast waves alone can cause mechanical insult that is comparable to existing traumatic brain injury thresholds is still an open question, and this work is aimed to address this issue.

In the first part of this dissertation, mechanics of primary blast loading on Realistic Explosive Dummy (RED) head with and without helmets is studied …

Evaluation Of The Thorax Of Manduca Sexta For Flapping Wing Micro Air Vehicle Applications, Brian C. Cranston

Theses and Dissertations

The tobacco hornworm hawkmoth (Manduca sexta) provides an excellent model from which to gather knowledge pertaining to the development of a Flapping Wing Micro Air Vehicle (FWMAV). One of the major challenges in design of a FWMAV is the energy demanding nature of low Reynolds number flapping flight. Therefore, an understanding of the power required by the flight muscles to actuate the wings is essential for the design of a FWMAV. The M.sexta wing/thorax mechanism was evaluated as a mechanical system in order to gain insight to the mechanical power required to produce the full natural wing stroke. A unique …

Machine Conscious Architecture For State Exploitation And Decision Making, Mark M. Derriso

Theses and Dissertations

This research addressed a critical limitation in the area of computational intelligence by developing a general purpose architecture for information processing and decision making. Traditional computational intelligence methods are best suited for well-defined problems with extensive, long-term knowledge of the environmental and operational conditions the system will encounter during operation. These traditional approaches typically generate quick answers (i.e., reflexive responses) using pattern recognition methods. Most pattern recognition techniques are static processes which consist of a predefined series of computations. For these pattern recognition approaches to be effective, training data is required from all anticipated environments and operating conditions. The proposed …

Emulation Of Industrial Control Field Device Protocols, Robert M. Jaromin

Theses and Dissertations

It has been shown that thousands of industrial control devices are exposed to the Internet, however, the extent and nature of attacks on such devices remains unknown. The first step to understanding security problems that face modern supervisory control and data acquisition (SCADA) and industrial controls networks is to understand the various attacks launched on Internet-connected field devices. This thesis describes the design and implementation of an industrial control emulator on a Gumstix single-board computer as a solution. This emulator acts as a decoy field device, or honeypot, intended to be probed and attacked via an Internet connection. Evaluation techniques …

Aerial Refueling Simulator Validation Using Operational Experimentation And Response Surface Methods With Time Series Responses, Alexander P. Hillman

Theses and Dissertations

An important program in the Department of Defense is the KC-46 Supertanker. Dubbed the future of the Air Force's aerial refueling inventory, the KC-46 will replace dozens of ailing previous generation tanker aircraft. The Aerial Refueling Airplane Simulator Qualification document governs the methods by which Air Mobility Command validates its simulators, some of which will be KC-46 simulators in the near future. The methodology set forward in this thesis utilizes historical data of aircraft performance from similar air frames to gain statistical insight into the performance design space of the KC-46. Leveraging this insight, the methodology provides through a framework …

Fate Of Malathion In An Activated Sludge Municipal Wastewater Treatment System, Edward B. Walters

Theses and Dissertations

Organophosphate compounds are used as pesticides and in chemical warfare agents such as nerve agents. VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate) is one of the most toxic and environmentally persistent of these nerve agents. This research examined the fate of malathion, a pesticide and surrogate of VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate), in bench-scale activated sludge (AS) sequencing batch reactors. Sorption kinetics and sorption equilibrium isotherm experiments indicate that sorption to AS biomass was not a statistically important removal mechanism. However, approximately 90% of the initial 4.25 mg L-1 malathion concentration degraded primarily via heterotrophic activity.

A Trophic State Analysis Of Lakes In Yellowstone National Park, Anthony Alexander Melcher

Theses and Dissertations

Eutrophication is of interest in the field of water quality. Eutrophic lakes, when used as sources for drinking water, can cause problems during the treatment process, for example algae blooms can clog filters, requiring more water and energy to be used during the cleaning and backwashing of the filters. Excess nutrient loading and eutrophication can also harm fish and aquatic life habitats. Certain species of algae and cyanobacteria can be toxic to humans as well. Since 1998, Dr. A. Woodruff Miller has collected water samples from 46 lakes and ponds in Yellowstone National Park. The Carlson Trophic State Index, the …

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• The New Kentucky Nitrogen and Phosphorus Risk Assessment Tool to Protect Water Quality, Tibor Horvath and others, USDA-NRCS, Lexington, KY
• Groundwater Phosphorus in Kentucky Relative to Karst, Groundwater Sensitivity, and Groundwater Physiographic Region, Caroline Chan, Kentucky Division of Water, Frankfort, KY
• Comparison of Irrigation Scheduling Based on Daily Water Use or Plant Water Demand of Container Grown Nursery Plants, Susmitha Nambuthiri and others, Dept of Horticulture, UK
• Arsenic Species in Broiler (Gallus gallus domesticus) Litter, Soils, Maize (Zea mays L.), and Groundwater from Litter-Amended Fields, Georgia Zeigler and others, Dept Plant and Soil Science, UK
• Cyberinfrastructure …

Session 2b, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Developing a Vegetation-Based Index of Biotic Integrity for Assessing the Ecological Condition of Wetlands in Kentucky, Tanner Morris and others, Dept of Biological Sciences, EKU, Richmond, KY
• Using Vegetation and Landscape Analysis to Validate a Wetland Rapid Assessment Method for Kentucky's Forested Riparian Wetlands, JohnRyan Polascik and others, Dept of Biological Sciences, EKU, Richmond, KY
• Improving Overall Stream Function Beyond the Channel (Opportunity Meets Necessity), Oakes Routt and Wanda Lawson, Stantec Consulting Services Inc., Louisville, KY
• The Effects of Prescribed Fire Restoration on Amphibian and Reptile Diversity, Robert Knopp and others, Murray State University, Murray, KY …

Session 1a, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Development and Testing of a New Phosphorus Index for Kentucky, Carl Bolster and others, USDA-ARS, Bowling Green, KY
• The New Kentucky Nitrogen and Phosphorus Risk Assessment Tool to Protect Water Quality, Tibor Horvath and others, USDA-NRCS, Lexington, KY
• Herbicide Transport Within Shallow Karst Groundwater on Kentucky's Pennyroyal Plateau Beneath Row Crop Agriculture, Chris Groves and others, Hoffman Environmental Research Institute, WKU, Bowling Green, KY
• Groundwater Tracing in the West Pennyrile Karst Region, Robert Blair and Jessica Moore, Kentucky Division of Water, Frankfort, KY

Proceedings Of 2013 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This symposium was planned and conducted as a part of the state water resources research institute annual program that is supported by Grant/Cooperative Agreement Number G11AP20081 from the United States Geological Survey. The contents of this proceedings document and the views and conclusions presented at the symposium are solely the responsibility of the individual authors and presenters and do not necessarily represent the official views of the USGS or of the symposium organizers and sponsors. This publication is produced with the understanding that the United States Government is authorized to reproduce and distribute reprints for government purposes.

Effect Of Cd44 Binding Peptide Conjugated To An Engineered Inert Matrix On Maintenance Of Breast Cancer Stem Cells And Tumorsphere Formation, Xiaoming Yang, Samaneh Kamali Sarvestani, Seyedsina Moeinzadeh, Xuezhong He, Esmaiel Jabbari

Faculty Publications

Introduction: As cancer cells are affected by many factors in their microenvironment, a major challenge is to isolate the effect of a specific factor on cancer stem cells (CSCs) while keeping other factors unchanged. We have developed a synthetic inert 3D polyethylene glycol diacrylate (PEGDA) gel culture system as a unique tool to study the effect of microenvironmental factors on CSCs response. We have reported that CSCs formed in the inert PEGDA gel by encapsulation of breast cancer cells maintain their stemness within a certain range of gel stiffness. The objective was to investigate the effect of CD44 binding peptide …

Design And Analysis Of A Small Form Factor Desktop Computer Enclosure, Curt Adkins

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Masters of Science Engineering Technology by Curt Adkins on March 13, 2013.

Willoughby Spit Shoreline Improvement Project Of Ocean View Beach, Maura Boswell

March 13, 2013: Regional Sea Level Rise Assessment, Adaptation and Flood Mitigation Projects

No abstract provided.

Method Of Purifying Crude Noscapine, Benjamin M. Marshall, Keith Tomazi

Chemical and Biochemical Engineering Faculty Research & Creative Works

The invention provides a method for separating noscapine from an opium source without substantially changing the color of the noscapine.

Design Visualization And Dynamic Assignment Of Traffic Volumes, Ting Wei, Paul Jarboe, Amanda Johnson

Purdue Road School

Design visualization for transportation projects is important for public outreach and facilitating stakeholder communications. Incorporating dynamic objects such as traffic elements can help in illustrating how the facility operates. This presentation will discuss the selection of design visualization products as well as best practices for their use. The presentation will also provide a discussion of Dynamic Assignment of Traffic Volumes (DTA) which takes driver expectations into consideration and provides a way for traffic engineers to evaluate traffic demand within a network based on impedance identified in the network.

Cpe 200: Small Bear Project, David Burke, Devin Tang

Computer Engineering

CPE 200 is an elective course designed for sophomores in the Computer Engineering Department. This project was intended to design the class, such that, students will understand the skills a computer engineer develops and how to apply those skills.

Gyroscope Assembly Process Improvement Analysis, Ian Douglass, Juan Ulises Saucedo

Industrial and Manufacturing Engineering

The gyro is a fundamental portion of many assemblies that Company S, a directional drilling company, manufactures. The gyro assembly is a highly sensitive and complex process, and it was paramount to establish efficiency and quality guidelines to base management decisions. The first guideline succeeded by increasing throughput by means of a simulation using ProModel. Next, non-value and value added activities were identified through a value stream map. Lastly, a database was developed in order to capture high integrity quality control data, or key performance indicators, such as, throughput, first-pass inspection yields at every test performed, and defects found. This …

Nanosecond Pulsed Electric Field Thresholds For Nanopore Formation In Neural Cells., Caleb C Roth, Gleb P Tolstykh, Jason A Payne, Marjorie A Kuipers, Gary L. Thompson Iii, Mauris N Desilva, Bennett L Ibey

Henry M. Rowan College of Engineering Departmental Research

The persistent influx of ions through nanopores created upon cellular exposure to nanosecond pulse electric fields (nsPEF) could be used to modulate neuronal function. One ion, calcium (Ca(2+)), is important to action potential firing and regulates many ion channels. However, uncontrolled hyper-excitability of neurons leads to Ca(2+) overload and neurodegeneration. Thus, to prevent unintended consequences of nsPEF-induced neural stimulation, knowledge of optimum exposure parameters is required. We determined the relationship between nsPEF exposure parameters (pulse width and amplitude) and nanopore formation in two cell types: rodent neuroblastoma (NG108) and mouse primary hippocampal neurons (PHN). We identified thresholds for nanoporation using …

Groundwater Levels In Northern Texas High Plains: Baseline For Existing Agricultural Management Practices, Jairo E. Hernández, Prasanna H. Gowda, Thomas H. Marek, Terry A. Howell, Wonsook Ha

Civil Engineering Faculty Publications and Presentations

New groundwater policies are being debated for the Northern Texas High Plains because of Ogallala Aquifer depletion. These policies should be evaluated using a calibrated groundwater model for assessing their impact on subsequent groundwater levels. The objective of this study was to calibrate and validate a regional groundwater model for predicting the impact of existing agricultural management practices on groundwater levels beneath 4 counties located in the Northern Texas High Plains. Results indicated that the MODFLOW-2000 groundwater model was calibrated and validated satisfactorily based on reproducing and comparing groundwater levels with coefficients of determination of 0.97 and 0.98, root mean …

Mitochondrial Handling Of Excess Ca2+ Is Substrate-Dependent With Implications For Reactive Oxygen Species Generation, Mohammed Aldakkak, David F. Stowe, Ranjan K. Dash, Amadou K.S. Camara

Biomedical Engineering Faculty Research and Publications

The mitochondrial electron transport chain is the major source of reactive oxygen species (ROS) during cardiac ischemia. Several mechanisms modulate ROS production; one is mitochondrial Ca²⁺ uptake. Here we sought to elucidate the effects of extramitochondrial Ca²⁺ (e[Ca²⁺]) on ROS production (measured as H₂O₂ release) from complexes I and III. Mitochondria isolated from guinea pig hearts were preincubated with increasing concentrations of CaCl₂ and then energized with the complex I substrate Na⁺ pyruvate or the complex II substrate Na⁺ succinate. Mitochondrial H₂O₂ release rates were assessed after …

Investigating The Reproducibility Of The Current Bvm Protocol, Corey Gross

Biomedical Engineering

Coronary Artery Disease (CAD) is responsible for 1 death every minute in the US. Angioplasty with the implantation of stents is a common treatment method for CAD. Although there is a variety of stents currently on the market, there is still a need to develop new types for different pathologic conditions. Preliminary assessment of the physiological response to new stents is needed as they are being developed. The FDA approval process implemented today is a long, tedious path with a range of testing methods that include static in vitro testing and high-cost animal testing. Tissue engineered blood vessels have been …

Mickey's Memory Game: A Children's Memory Game Designed For Sifteo Cubes, Anjelica Concepcion

Computer Engineering

Mickey’s Memory Game is a children’s memory game developed on the Sifteo platform. Sifteo cubes are small, interactive devices, which can display colored images and sense neighboring cubes or detect user actions, such as clicking, shaking, or tilting the cubes. Although many classic video games or mobile games can be recreated on the Sifteo cubes, the goal of this project was to utilize capabilities that are unique to the Sifteo gaming platform, while also creating a useful game for child development.

Single-Cell Measurements Of Ige-Mediated Fcεri Signaling Using An Integrated Microfluidic Platform, Yanli Liu, Dipak Barua, Peng Liu, Bridget S. Wilson, Janet M. Oliver, William S. Hlavacek, Anup K. Singh

Chemical and Biochemical Engineering Faculty Research & Creative Works

Heterogeneity in responses of cells to a stimulus, such as a pathogen or allergen, can potentially play an important role in deciding the fate of the responding cell population and the overall systemic response. Measuring heterogeneous responses requires tools capable of interrogating individual cells. Cell signaling studies commonly do not have single-cell resolution because of the limitations of techniques used such as Westerns, ELISAs, mass spectrometry, and DNA microarrays. Microfluidics devices are increasingly being used to overcome these limitations. Here, we report on a microfluidic platform for cell signaling analysis that combines two orthogonal single-cell measurement technologies: on-chip flow cytometry …

Effects Of Ovariectomy And Anatomical Location On Osteonal Encroachment In Adult Cortical Ovine Bone, Paige Brell Ryan

Master's Theses

The purpose of this study is to further quantify adult ovine ovariectomized bone for new remodeling characteristics to obtain a better understanding of how remodeling is occurring and the effectiveness of this animal model for the study of postmenopausal osteoporosis. Postmenopausal osteoporosis is a major health concern and animal models to test new treatment options are needed. The ovine model is a good option because the ewes undergo Haversian remodeling, are a large sized animal, and have a similar hormone profile to humans. Ewes, however, do not undergo a natural menopause, so an ovariectomy surgery was conducted in the sheep …

Hydrologic Modeling Of The San Joaquin Valley Watershed For Purposes Of Nitrate Analysis, Stephen Carl Clayton

Master's Theses

The San Joaquin Valley is regarded as one of the most productive agricultural regions in the world. This extensive agriculture has, however, caused extensive pollution of both ground water and surface water. This thesis develops a hydrologic model of the surface and ground waters of the San Joaquin Valley. Such modeling is useful in the development and implementation of water quality regulations such as Total Maximum Daily Loads (TMDLs). A properly validated watershed simulation model can supplement data collection and can account for watershed characteristics including topography, soils, climate, land cover, anthropogenic activities, as well as simulate watershed responses including …

Three-Dimensional Engineered Matrix To Study Cancer Stem Cells And Tumorsphere Formation: Effect Of Matrix Modulus, Xiaoming Yang, Samaneh Kamali Sarvestani, Seyedsina Moeinzadeh, Xuezhong He, Esmaiel Jabbari

Faculty Publications

Maintenance of cancer stem cells (CSCs) is regulated by the tumor microenvironment. Synthetic hydrogels provide the flexibility to design three-dimensional (3D) matrices to isolate and study individual factors in the tumor microenvironment. The objective of this work was to investigate the effect of matrix modulus on tumorsphere formation by breast cancer cells and maintenance of CSCs in an inert microenvironment without the interference of other factors. In that regard, 4T1 mouse breast cancer cells were encapsulated in inert polyethylene glycol diacrylate hydrogels and the effect of matrix modulus on tumorsphere formation and expression of CSC markers was investigated. The gel …

Discrimination Between Child And Adult Forms Using Radar Frequency Signature Analysis, Stephanie R. Keith

Theses and Dissertations

In this thesis we develop a method to discriminate between adult and child radar signatures. In particular, we examine radar data measured from behind a wall, which introduces radar signal attenuation and multipath effects. To investigate the child/adult discrimination problem in a through-wall, multipath scenario, a previously developed free-space human scattering model was expanded to incorporate multiple paths, and the effects of transmission through, and reflections from, walls and ground. The ground was modeled as a perfectly reflecting surface, while the walls were modeled as homogeneous concrete slabs. Twenty-five reflection paths were identified, involving the direct paths, as well as …

Discrimination Of Individual Tigers (Panthera Tigris) From Long Distance Roars, An Ji, Michael T. Johnson, Edward J. Walsh, Joann Mcgee, Douglas L. Armstrong

Electrical and Computer Engineering Faculty Research and Publications

This paper investigates the extent of tiger (Panthera tigris) vocal individuality through both qualitative and quantitative approaches using long distance roars from six individual tigers at Omaha's Henry Doorly Zoo in Omaha, NE. The framework for comparison across individuals includes statistical and discriminant function analysis across whole vocalization measures and statistical pattern classification using a hidden Markov model (HMM) with frame-based spectral features comprised of Greenwood frequency cepstral coefficients. Individual discrimination accuracy is evaluated as a function of spectral model complexity, represented by the number of mixtures in the underlying Gaussian mixture model (GMM), and temporal model complexity, …