Digital Commons Network^™

Physics Trick - Balanced Can, Said Shakerin

All Faculty Articles - School of Engineering and Computer Science

The ordinary 12-oz beverage cans in the figures below are not held up with any props or glue. The bottom of such cans is stepped at its circumference for better stacking. When this kind of can is tilted, as shown in Fig. 1, the outside corners of the step touch the surface beneath, providing an effective contact about 1 cm wide. Because the contact is relatively wide and the geometry is symmetrical, it is easy to balance an empty can by simply adding an appropriate amount of water so that the overall center of mass is located directly above the …

Raman Spectroscopic Investigation Of Chondroitinase Abc Treatment After Spinal Cord Injury In An Organotypic Model, Shane Ruberto

Theses - ALL

This study investigated (1) the ability of an organotypic model of spinal cord injury (SCI) to recapitulate significant pathobiological events of in vivo SCI from a Raman spectroscopic perspective, and (2) the Raman spectroscopic effects of chondroitinase ABC (cABC) treatment on said model of SCI. Tissue from mouse spinal cords was excised, sectioned, and placed into culture, before being systematically injured to induce trauma. Cultures with and without cABC treatment were assessed using Raman spectroscopy at varying times post-injury. We demonstrate that Raman spectral phenomena characteristic of SCI can be observed using an organotypic model. By comparing these phenomena with …

Electrotransfer Of Single-Stranded Or Double-Stranded Dna Induces Complete Regression Of Palpable B16.F10 Mouse Melanomas, Loree Heller, Vesba Todorovic, Maja Cemazar

Bioelectrics Publications

Enhanced tumor delivery of plasmid DNA with electric pulses in vivo has been confirmed in many preclinical models. Intratumor electrotransfer of plasmids encoding therapeutic molecules has reached Phase II clinical trials. In multiple preclinical studies, a reduction in tumor growth, increased survival or complete tumor regression have been observed in control groups in which vector or backbone plasmid DNA electrotransfer was performed. This study explores factors that could produce this antitumor effect. The specific electrotransfer pulse protocol employed significantly potentiated the regression. Tumor regression was observed after delivery of single-stranded or double-stranded DNA with or without CpG motifs in both …

Design Of A Low Power Cargo Security Device Using A Micropower Ultra-Wideband Impulse Radar, Brian M. Wihl

Master's Theses

Each year, thousands of cargo containers are broken into during shipping, costing billions of dollars in lost and damaged goods. In addition to removing its contents, intruders can also add unwanted and dangerous materials to a container, posing a threat to National Security. The possibilities of cargo container break-ins require that the containers go through check points at which they are physically searched. These searches often require the opening of the container, unloading and inspecting all cargo, and then loading the container and resealing it. This is a long and costly process.

Because of the high costs of break-ins and …

Near-Infrared Surface-Enhanced Fluorescence Using Silver Nanoparticles In Solution, Michael D. Furtaw

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Fluorescence spectroscopy is a widely used detection technology in many research and clinical assays. Further improvement to assay sensitivity may enable earlier diagnosis of disease, novel biomarker discovery, and ultimately, improved outcomes of clinical care along with reduction in costs. Near-infrared, surface-enhanced fluorescence (NIR-SEF) is a promising approach to improve assay sensitivity via simultaneous increase in signal with a reduction in background. This dissertation describes research conducted with the overall goal to determine the extent to which fluorescence in solution may be enhanced by altering specific variables involved in the formation of plasmonactive nanostructures of dye-labeled protein and silver nanoparticles …

Clustering And Classification Of Multi-Domain Proteins, Neethu Shah

School of Computing: Dissertations, Theses, and Student Research

Rapid development of next-generation sequencing technology has led to an unprecedented growth in protein sequence data repositories over the last decade. Majority of these proteins lack structural and functional characterization. This necessitates design and development of fast, efficient, and sensitive computational tools and algorithms that can classify these proteins into functionally coherent groups.

Domains are fundamental units of protein structure and function. Multi-domain proteins are extremely complex as opposed to proteins that have single or no domains. They exhibit network-like complex evolutionary events such as domain shuffling, domain loss, and domain gain. These events therefore, cannot be represented in the …

The Effect Of Skew Angle On Average Queue Delay At Tee-Intersections: A Simulation Study Using The Texas Model, Manogna Kaluva

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

This thesis explores the effect of intersection skew angle on average queue delay through simulation. The simulation model used is the TEXAS (Traffic Experimental and Analytical Simulation) model. This microscopic simulation model uses a general nonlinear car-following model. It simulates individual intersections and was designed to capture the interaction of traffic operations and intersection geometry.

Simulation models were developed for three stop-controlled, tee-intersections in Lincoln, Nebraska. Field data to develop and calibrate the simulation models were collected. All simulation models were calibrated by adjusting the car following parameters. An experimental design was developed to test the effect of skew angle …

Development Of A Field Test For Total Suspended Solids Analysis, Jessica Branigan

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Total suspended solids (TSS) are all particles in water that will not pass through a glass fiber filter with a pore size less than 2 μm, including sediments, algae, nutrients, and metals. TSS is an important water quality parameter because of its adverse effects on aquatic species and wildlife. The EPA has proposed a regulation for turbidity, a related water quality parameter, which has been stayed pending further testing. TSS is regulated through the EPA via the NPDES in many states. Since there are no accepted field tests for TSS, projects with TSS regulations must send samples to a laboratory …

Functional Stroke Recovery Through Tissue Engineered Niche Neural Constructs, Natasha Topoluk

All Theses

According to the Centers for Disease Control and Prevention, stroke is statistically responsible for 1 in every 19 deaths of American citizens.5 Stroke is the leading cause of permanent disability due to the fact that it compromises both cellular and tissue components of the brain, leading to the formation of a physical void within the tissue. Current research approaches address the cellular component by injecting stem cells into this void; however, extremely low cell engraftment, high injected cell death, and overall no matrix regeneration are observed. Additionally, these stem cells do not remain committed to a neural lineage. We propose …

Chemical, Physical, And Moisture Release Characteristics Of Compost Based Potting Media, Tianzhen Wang

All Theses

A commercially available composted cow manure was used as a potting media amendment. A common base mix for woody ornamentals of 8 parts pine bark and 1 part sand was compared with 3 other mixes that contained 20%, 30%, and 40% compost (v/v). The remaining fraction of these three mixes was the base mix (bark and sand). Samples of the pine bark and compost were analyzed to determine the following plant nutrients and mineral concentrations: nitrogen (organic and soluble), carbon, P2O5, K2O, Ca, S, Mg, Mn, Cu, Zn, Na, Fe, and Al.

Structural Characterization Of Super-Acid Oligomerized Fatty Esters., Esam Allehyani

Electronic Theses & Dissertations

Fatty acid methyl esters can be oligomerized with very strong acid catalysts - superacids such as tetrafluoroboric or triflic acid with formation of dimer and trimer fatty acids at much lower temperature, such as 80-90 °C, than what is used presently. Insight into the reaction of polymerization of fatty esters/ acids can be obtained by structural characterization. This would determine the properties of the oligomeric products and their ability to substitute currently used materials. It was presumed that dimer fatty, trimer fatty acid esters, and oligomeric fatty acid esters are produced by polymerization of fatty acid methyl esters by a …

Research: South Dakota State University, Winter 2013, Christie Delfanian, Matt Landwehr

State of Discovery South Dakota State University's Research Magazine

CONTENTS:

South Dakota expertise integral part of Landsat [Page] 1
West Nile: Researchers help public health officials combat West Nile virus [page] 2
Health care: arteries, corticosteroids, concussions, and preemies [Page] 4
Oilseeds [Page] 6
Undergraduate Research [Page] 8
Organ donation key to helping Native Americans on dialysis [Page] 10
Researchers meet challenge of increased competition: 2012 measurements [Page] 11

Sodhana Environmental - Final Design Report, Eric Taylor, Corissa Bellis, Cody Perez, Cameron Zeller

Mechanical Engineering

A fully designed cost-effective, sustainable, toilet for the developing world.

Development And Validation Of An Absorption Sensor For Time-Resolved Measurements Of Co And Co2, Kyle Thurmond

HIM 1990-2015

A sensor was developed for simultaneous measurements of carbon monoxide and carbon dioxide fluctuations in internal combustion engine exhaust gases. This sensor utilizes low-cost and compact LEDs that emit in the 3-5[micro]m wavelength range which are more appropriate for practical applications than the more traditionally used lasers. An affordable, fast response sensor that can measure these gases has broad application that can lead to more efficient, fuel flexible engines and regulations of harmful emissions. LEDs have a more spectrally broad and diverging emission than lasers which presented many design challenges. The optical design software ZEMAX was utilized to overcome these …

Assessing Different Zeolitic Adsorbents For Their Potential Use In Kr And Xe Separation, Breetha Alagappan

UNLV Theses, Dissertations, Professional Papers, and Capstones

Separation of Kr from Xe is an important problem in spent nuclear fuel fission gas management. The energy intensive and expensive cryogenic distillation method is currently used to separate these gases. In this thesis, we have carried out the research into appropriate sorbents for the separation of Kr and Xe using pressure swing adsorption. We have examined zeolites using gas adsorption studies as they have the potential to be more cost effective than other sorbents. Zeolites are microporous aluminosilicates and have ordered pore structures. The pores in zeolites have extra-framework cations are substantially free to move. The mobility of cations …

Manufacturing The Next Generation Of Vaccines: Non-Egg Based Platform For Influenza Vaccine, Andrew B. Price

Chemical Engineering Undergraduate Honors Theses

This report explores the design of an influenza vaccine manufacturing facility using a non-egg based expression platform. The current standard procedure in flu vaccine production is to grow the virus in bird eggs. However, recently there is evidence that supports production by other expression platforms. The Sf9 insect cell is considered in this report. Facility design including cell culture media formulation through product storage for shipment and final formulation is considered based on the use of an existing facility. The new design also employs the use of disposable equipment such as bioreactors and purification unit operations. These disposables help reduce …

Implementation Of Precast Concrete Deck System Nudeck (2nd Generation), George Morcous, Maher K. Tadros

Nebraska Department of Transportation: Research Reports

No abstract provided.

Development And Evaluation If Best Management Practices For Highway Runoff Pollution Control, Tian C. Zhang, John S. Stansbury, Massom Moussavi

Nebraska Department of Transportation: Research Reports

No abstract provided.

Lipid Production By Microalgae Treating Municipal Wastewater, James Edward Kelley

Master's Theses

Microalgae hold much promise as a feedstock in liquid biofuel production. Lipid content of microalgae cells range from 30-80% dry weight of biomass. It is projected that microalgae can produce between 1,000-6,500 gallons/acre/year of oil. Currently, production of industrial algae operates in open raceway ponds that use minimal capital and energy inputs to culture algae. Raceway ponds can also be used to grow microalgae from municipal waste streams. Although high biomass productivity can be achieved in these systems, there remains a large production gap between large volumes of biomass cultivation and high lipid content from microalgae cells. Low lipid content …

Large-Displacement Lightweight Armor, Eric C. Clough

Master's Theses

Randomly entangled fibers forming loosely bound nonwoven structures are evaluated for use in lightweight armor applications. These materials sacrifice volumetric efficiency in order to realize a reduction in mass versus traditional armor materials, while maintaining equivalent ballistic performance. The primary material characterized, polyester fiberfill, is shown to have improved ballistic performance over control samples of monolithic polyester as well as 1095 steel sheets. The response of fiberfill is investigated at a variety of strain rates, from quasistatic to ballistic, under compression, tension, and shear deformation to elucidate mechanisms at work during ballistic defeat. Fiberfill’s primary mechanisms during loading are fiber …

Mechanical Properties Of Bone Due To Sost Expression: Nanoindentation Assessment Of Murine Femurs, Amir Rafie

Master's Theses

In the human genome, the SOST gene codes for a protein sclerostin. Sclerostin is an osteocyte-expressed negative regulator of bone formation. When the SOST gene is not coded, bone formation is reduced in individuals during skeletal maturation. This study utilizes nanoindentation methods to test for the mechanical properties of bones that both express and do not express the SOST gene. 100 transgenic murine femurs were obtained from Lawrence Livermore Labs in the form of 6 and 8 month SOST transgenic mice, 6 and 12 month SOST knockout mice, and wild type control littermates for each of the 4 age groups. …

Parsing Of Natural Language Requirements, Jamie L. Patterson

Master's Theses

The purpose of this thesis was to automate verification of the software requirements for an implantable cardioverter defibrillator with minimal manual rework. The requirements were written in plain English with only loose stylistic constraints. While full automation proved infeasible, many significant advances were made towards solving the problem, including a framework for storing requirements, a program which translates most of the natural language requirements into the framework, and a novel approach to parts of speech analysis.

Vascular Nanomedicine: Site Specific Delivery Of Elastin Stabilizing Therapeutics To Damaged Arteries, Aditi Sinha

All Dissertations

Elastin, a structural protein in the extra-cellular matrix, plays a critical role in the normal functioning of blood vessels. Apart from performing its primary function of providing resilience to arteries, it also plays major role in regulating cell-cell and cell-matrix interactions, response to injury, and morphogenesis. Medial arterial calcification (MAC) and abdominal aortic aneurysm (AAA) are two diseases where the structural and functional integrity of elastin is severely compromised. Although the clinical presentation of MAC and AAA differ, they have one common underlying causative mechanism--pathological degradation of elastin. Hence prevention of elastin degradation in the early stages of MAC and …

Biological Scaffolds For Peripheral Vascular Surgery, George Fercana

All Dissertations

The gold standards for small diameter peripheral vascular graft replacement are autologous arteries or veins; however, one-third of patients lack such vessels due to previous vessel harvesting or advanced vascular disease. A promising approach for patients in this category is tissue engineering with off-the-shelf biological vascular grafts. Three small diameter acellular scaffolds were developed and evaluated as vascular grafts. Porcine renal arteries (2-3 mm diameter, 20 mm length) were decellularized by immersion and stabilized with penta-galloyl glucose (PGG) with and without subsequent heparinization via carbodiimide chemistry. Bovine mammary (4-6 mm ID, 250 mm length) and femoral arteries (6-8 mm ID, …

Depleted Energy Charge And Increased Pulmonary Endothelial Permeability Induced By Mitochondrial Complex I Inhibition Are Mitigated By Coenzyme Q1 In The Isolated Perfused Rat Lung, Robert D. Bongard, Ke Yan, Raymond G. Hoffmann, Said H. Audi, Xiao Zhang, Brian J. Lindemer, Mary I. Townsley, Marilyn P. Merker

Biomedical Engineering Faculty Research and Publications

Mitochondrial dysfunction is associated with various forms of lung injury and disease that also involve alterations in pulmonary endothelial permeability, but the relationship, if any, between the two is not well understood. This question was addressed by perfusing isolated intact rat lung with a buffered physiological saline solution in the absence or presence of the mitochondrial complex I inhibitor rotenone (20 μM). Compared to control, rotenone depressed whole lung tissue ATP from 5.66±0.46 (SEM) to 2.34±0.15 µmol·g⁻¹ dry lung, with concomitant increases in the ADP:ATP and AMP:ATP ratios. Rotenone also increased lung perfusate lactate (from 12.36±1.64 to 38.62±3.14 µmol·15 …

Improving Safety In Medical Devices From Concept To Retirements, Martin Mchugh, Fergal Mccaffery, Silvana Macmahon, Anita Finnega

Books/Book Chapters

As with many domains the use of software within the healthcare industry is on the rise [1, 2] within the last 20 years.

Aerodynamic Performance And Particle Image Velocimetery Of Piezo Actuated Biomimetic Manduca Sexta Engineered Wings Towards The Design And Application Of A Flapping Wing Flight Vehicle, Anthony M. Deluca

Theses and Dissertations

Considerable research and investigation has been conducted on the aerodynamic performance, and the predominate flow physics of the Manduca sexta size of biomimetically designed and fabricated wings as part of the AFIT FWMAV design project. Despite a burgeoning interest and research into the diverse field of flapping wing flight and biomimicry, the aerodynamics of flapping wing flight remains a nebulous field of science with considerable variance into the theoretical abstractions surrounding aerodynamic mechanisms responsible for aerial performance. Traditional FWMAV flight models assume a form of a quasi-steady approximation of wing aerodynamics based on an infinite wing element model (BEM). An …

Engineering Beta-Cell Spheroids For Type 1 Diabetes Treatment, Xiaoyan Liu

All Dissertations

Diabetes mellitus, the third most common disease in the world, is a chronic metabolic disorder caused by a failure of insulin production and/or an inability to respond to insulin. Specifically, type 1 diabetes is a disorder characterized by targeted autoimmune-directed destruction of a patient's beta-cell population within the pancreatic islets of Langerhans. The current primary treatment for type 1 diabetes is daily multiple insulin injections. However, this treatment cannot provide sustained physiological release, and the insulin amount is not finely tuned to glycemia. Pancreatic transplants or islet transplants would be the preferred treatment method but the lack of donor tissue …

Identification Of Novel Genes Regulating Elastic Fiber Formation Through Expression Profiling Analysis Of Elastogenic Models, Erin Sproul

All Dissertations

Background: Particularly important to the mechanical performance of native arterial blood vessels is elastin, an extracellular matrix (ECM) protein deposited by VSMCs in the form of elastic fibers, arranged in concentric lamellae in the media of the vessel wall. In addition to serving as major structural elements of arterial walls, providing extensibility and elastic recoil, elastic fibers also influence vascular cell behaviors. For these reasons tissue engineers are attempting to exploit elastic fiber biology to enhance vascular graft design and patency. Therefore, developing a greater understanding of the molecular mechanisms of elastogenesis may offer opportunities to control elastogenesis in tissue …

Evaluation Of Alternate Mouthpiece Material Types To Minimize Vibrations And Heat Loss - The Research And Execution Of Prototypes, Kristen Bobuk

Theses, Dissertations and Capstones

This paper is based on the idea of current horn mouthpieces being made solely out of brass with a limited market of stainless steel and titanium with plating being silver or gold. This is an opportunity to explore alternate materials; specifically steel, cast iron and aluminum for the base material and chrome, copper and nickel for plating materials. Readers will learn about metal properties such as hardness, thermal conductivity and specific heat and learn why certain materials are more beneficial than others.