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A Label-Free High Throughput Resistive-Pulse Sensor For Simultaneous Differentiation And Measurement Of Multiple Particle-Laden Analytes, Ashish Jagtiani, Rupesh Sawant, Jiang Zhe
A Label-Free High Throughput Resistive-Pulse Sensor For Simultaneous Differentiation And Measurement Of Multiple Particle-Laden Analytes, Ashish Jagtiani, Rupesh Sawant, Jiang Zhe
Dr. Jiang Zhe
We describe an all-electronic, label-free, resistive-pulse sensor that utilizes multiple microchannels for parallel detection, counting and differentiation of multiple biological particles simultaneously. Four particle solutions, including 20 µm and 40 µm polymethacrylate particles, Juniper Scopulorum (Rocky Mountain Juniper) pollen and Populus deltidoes (Eastern Cottonwood) pollen, were loaded to the four peripheral reservoirs, respectively, and were driven to the central reservoir through four microchannels, all operating simultaneously for particle detection and counting. Experiments demonstrated that this sensor was able to differentiate and count multiple particle solutions simultaneously through its four microchannels fabricated on polymer membranes. Thus the sensing throughput has been …
A New Microfluidic Device For Complete, Continuous Separation Of Microparticles, Liang-Liang Fan, Xu-Kun He, Yu Han, Li Du, Liang Zhao, Jiang Zhe
A New Microfluidic Device For Complete, Continuous Separation Of Microparticles, Liang-Liang Fan, Xu-Kun He, Yu Han, Li Du, Liang Zhao, Jiang Zhe
Dr. Jiang Zhe
A microchannel with symmetric sharp corners is reported for particle separation, based on the inter-play between the inertial lift force and the centrifugal force induced by sharp corners. At an appropriate flow rate, the centrifugal force is larger than the inertial lift force on large particles, while the inertial lift force is dominant on small particles. Hence large particles are centrifuged to the center, while small par-ticles are focused at side streams, achieving complete particle separation. The device requires no sheath flow, avoiding the dilution of analyte sample and complex operation, and can be potentially used for many lab-on-a-chip applications.
Detection And Counting Of Micro Scale Particles And Pollen Using A Multi-Aperture Coulter Counter, Ashish Jagtiani, Jiang Zhe, Jun Hu, Joan Carletta
Detection And Counting Of Micro Scale Particles And Pollen Using A Multi-Aperture Coulter Counter, Ashish Jagtiani, Jiang Zhe, Jun Hu, Joan Carletta
Dr. Jiang Zhe
We demonstrate a high throughput, all-electronic Coulter-type sensor with four sensing microapertures to detect and count micro-scale particles. Four particle samples are utilized for this study: polymethacrylate particles 40 µm and 20 µm in diameter, Juniper Scopulorum (Rocky Mountain Juniper) pollen and Cottonwood pollen particles. The two types of pollen particles are roughly 20 µm in diameter. The particles are mixed with deionized water and forced to pass through the microapertures. Voltage pulses across all four apertures are recorded and analysed. Results demonstrate that the sensor can detect and count particles through its four sensing apertures simultaneously. Thus, the counting …
Novel Quantitative Macro Biomolecule Analysis Based On A Micro Coulter Counter, Y Han, H. Wu, F. Liu, Gang Cheng, Jiang Zhe
Novel Quantitative Macro Biomolecule Analysis Based On A Micro Coulter Counter, Y Han, H. Wu, F. Liu, Gang Cheng, Jiang Zhe
Dr. Jiang Zhe
We demonstrate quantitative biomolecule analysis using a micro coulter counter. Specific binding between antibody functionalized microparticles and target biomolecule cause large aggregates of microparticles. The micro coulter counter was employed to measure aggregation ratio, ratio of the aggregate counts to the total particle counts. Goat anti-rabbit IgG, used as a model biomarker, were tested in this paper. The experiment results showed that the aggregation ratio increases with the increasing biomolecule concentration, and the detectable concentration range from 16 to 160 ng/ml was achieved.
Static And Dynamic Responsive Behavior Of Polyelectrolyte Brushes Under External Electrical Field, Hui Ouyang, Zhenhai Xia, Jiang Zhe
Static And Dynamic Responsive Behavior Of Polyelectrolyte Brushes Under External Electrical Field, Hui Ouyang, Zhenhai Xia, Jiang Zhe
Dr. Jiang Zhe
The static and dynamic behaviors of partially charged and end-grafted polyelectrolyte brushes in response to electric fields were investigated by means of molecular dynamics simulation. The results show that the polymer brushes can be partially or fully stretched by applying an external electric field. Moreover, the brushes can switch reversibly from collapsed to stretched states, fully responding to the AC electric stimuli, and the gating response frequency can reach a few hundred MHz. The effects of the grafting density, the charge fraction of the brushes and the strength of the electric field on the average height of the polymer brushes …
High School Bridge Program: A Multidisciplinary Stem Research Program, Jiang Zhe, Dennis Doverspike, Julie Zhao, Paul C. Lam, Craig C. Menzemer
High School Bridge Program: A Multidisciplinary Stem Research Program, Jiang Zhe, Dennis Doverspike, Julie Zhao, Paul C. Lam, Craig C. Menzemer
Dr. Jiang Zhe
A Science, Technology, Engineering and Math (STEM) summer Bridge Program was developed for high school students. The program was designed to encourage students to consider choosing an engineering major in college and to explore STEM as a future career. This was accomplished through a 10-week program involving multidisciplinary research activities. The participants in the program included 33 high school students. Among former participants in position to make a choice in terms of attending college, 100% had chosen to continue on in college, and 86% had chosen to major in a STEM area. The results indicated that that the program had …
An Evaluation Of A Stem Program For Middle School Students On Learning Disability Related Ieps, Paul C. Lam, Dennis Doverspike, Julie Zhao, Jiang Zhe, Craig C. Menzemer
An Evaluation Of A Stem Program For Middle School Students On Learning Disability Related Ieps, Paul C. Lam, Dennis Doverspike, Julie Zhao, Jiang Zhe, Craig C. Menzemer
Dr. Jiang Zhe
A year long Science, Technology, Engineering and Math (STEM) program was developed for middle schools students on Individualized Education Programs (IEPs) involving learning disabilities. The workshops were designed to encourage students both on IEPs and not on IEPs to explore STEM as a future career choice by building their knowledge and confidence. The participants in the workshops included 11 students on IEPs and 15 students not on IEPs. Parents also provided feedback regarding their attitudes toward the program. The results indicated that there were increases in student participant knowledge and career interest for both the students not on IEPs and …
Passive Continuous Particle Focusing In A Microchannel With Symmetric Sharp Corner Structures, Liang-Liang Fan, Liang Zhao, Xu-Kun He, Hand Yu, Qing-Yu Wei, Jiang Zhe
Passive Continuous Particle Focusing In A Microchannel With Symmetric Sharp Corner Structures, Liang-Liang Fan, Liang Zhao, Xu-Kun He, Hand Yu, Qing-Yu Wei, Jiang Zhe
Dr. Jiang Zhe
We report a continuous passive particle focusing method using a novel microchannel with symmetric sharp corners which induce curved streamlines and large centrifugal force on particles. At appropriate flow rate, the centrifugal force generated on particles exceeds the inertial lift force; particles driven by the centrifugal force migrate toward the center of the microchannel, achieving continuous particle focus-ing. With simple structure and operation, this method can be potentially used in particle focusing and ex-traction processes in a variety of lab-on-a chip applications.
Effects Of Particle's Off-Axis Position, Shape, Orientation And Entry Position On Resistance Changes Of Micro Coulter Counting Devices, Zhenpeng Qin, Jiang Zhe, Guo-Xiang Wang
Effects Of Particle's Off-Axis Position, Shape, Orientation And Entry Position On Resistance Changes Of Micro Coulter Counting Devices, Zhenpeng Qin, Jiang Zhe, Guo-Xiang Wang
Dr. Jiang Zhe
With the recent advance in micro/nano-fabrication technology, micro Coulter counters have been widely used in detecting and characterizing micro- and nanoscale objects. In this paper, the electrical resistance change during translocation of a non-conducting particle through a channel is studied numerically. The numerical results are validated by proven analytical results available in the literature. The effects of particle's off-axis position, shape and orientation, and entry position are studied for particles with a large dynamic range. From the numerical results, a new fitted correlation is proposed that can accurately predict the resistance change caused by off-axis spherical particles regardless of their …
A Versatile Microparticle-Based Immunoaggregation Assay For Macromolecular Biomarker Detection And Quantification, Haiyan Wu, Yu Han, George G. Chase, Qiong Tang, Chen-Jung Lee, Bin Cao, Jiang Zhe, Gang Cheng
A Versatile Microparticle-Based Immunoaggregation Assay For Macromolecular Biomarker Detection And Quantification, Haiyan Wu, Yu Han, George G. Chase, Qiong Tang, Chen-Jung Lee, Bin Cao, Jiang Zhe, Gang Cheng
Dr. Jiang Zhe
The rapid, sensitive and low-cost detection of macromolecular biomarkers is critical in clinical diagnostics, environmental monitoring, research, etc. Conventional assay methods usually require bulky, expensive and designated instruments and relative long assay time. For hospitals and laboratories that lack immediate access to analytical instruments, fast and low-cost assay methods for the detection of macromolecular biomarkers are urgently needed. In this work, we developed a versatile microparticle (MP)-based immunoaggregation method for the detection and quantification of macromolecular biomarkers. Antibodies (Abs) were firstly conjugated to MP through streptavidin-biotin interaction; the addition of macromolecular biomarkers caused the aggregation of Ab-MPs, which were subsequently …