Digital Commons Network^™

Optimal Spatial Prioritization Of Control Resources For Elimination Of Invasive Species Under Demographic Uncertainty, Kim M. Pepin, Timothy J. Smyser, Amy J. Davis, Ryan S. Miller, Sophie Mckee, Kurt C. Vercauteren, William Kendall, Chris Slootmaker

United States Department of Agriculture Wildlife Services: Staff Publications

Populations of invasive species often spread heterogeneously across a landscape, consisting of local populations that cluster in space but are connected by dispersal. A fundamental dilemma for invasive species control is how to optimally allocate limited fiscal resources across local populations. Theoretical work based on perfect knowledge of demographic connectivity suggests that targeting local populations from which migrants originate (sources) can be optimal. However, demographic processes such as abundance and dispersal can be highly uncertain, and the relationship between local population density and damage costs (damage function) is rarely known. We used a metapopulation model to understand how budget and …

A Comparison Of Cost And Quality Of Three Methods For Estimating Density For Wild Pig (Sus Scrofa), Amy J. Davis, David A. Keiter, Elizabeth M. Kierepka, C. Slootmaker, Antoinette J. Piaggio, James C. Beasley, K. M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

A critical element in effective wildlife management is monitoring the status of wildlife populations; however, resources to monitor wildlife populations are typically limited. We compared cost effectiveness of three common population estimation methods (i.e. non-invasive DNA sampling, camera sampling, and sampling from trapping) by applying them to wild pigs (Sus scrofa) across three habitats in South Carolina, U.S.A where they are invasive. We used mark-recapture analyses for fecal DNA sampling data, spatially-explicit capture-recapture analyses for camera sampling data, and a removal analysis for removal sampling from trap data. Density estimates were similar across methods. Camera sampling was the least expensive, …

Technical Limitations Associated With Molecular Barcoding Of Arthropod Bloodmeals Taken From North American Deer Species, Erin M. Borland, Daniel A. Hartman, Matthew W. Hopken, Antoinette J. Piaggio, Rebekah C. Kading

United States Department of Agriculture Wildlife Services: Staff Publications

Accurate species-level identification of the source of arthropod bloodmeals is important for deciphering blood feeding patterns of field-collected specimens. Cytochrome c oxidase I (COI) mitochondrial gene sequencing has been used for this purpose; however, species resolution can be difficult to obtain from certain vertebrate genera, including Odocoileus. Sanger sequencing of mitochondrial genes was employed to identify the bloodmeal source of wild-caught mosquitoes trapped in Greeley, Colorado. Initial sequencing of the COI gene of mitochondrial DNA in bloodmeals was inadequate for species-level resolution of bloodmeals from deer in the genus Odocoileus, with current databases returning low fidelity matches to …

Additive And Non-Additive Responses Of Seedlings To Simulated Herbivory And Drought, Kasey Barton, Aaron B. Shiels

United States Department of Agriculture Wildlife Services: Staff Publications

Drought is a global threat, increasing in severity and frequency throughout tropical ecosystems. Although plants often face drought in conjunction with biotic stressors, such as herbivory or disease, experimental studies infrequently test the simultaneous effects of drought and biotic stress. Because multiple simultaneous stressors may have non-additive and complex effects on plant performance, it is difficult to predict plant responses to multiple threats from research examining one stress at a time. Using an experimental approach in the greenhouse, we investigated potential non-additivity in seedling growth and survival to simulated drought and herbivory across a phylogenetically diverse pool of ten Hawaiian …

The Changing Triad Of Plague In Uganda: Invasive Black Rats (Rattus Rattus), Indigenous Small Mammals, And Their Fleas, Russell E. Enscore, Nackson Babi, Gerald Amatre, Linda Atiku, Rebecca J. Eisen, Kim M. Pepin, Rommelle Vera-Tudela, Christopher Sexton, Kenneth L. Gage

United States Department of Agriculture Wildlife Services: Staff Publications

Rattus rattus was first reported from the West Nile Region of Uganda in 1961, an event that preceded the appearance of the first documented human plague outbreak in 1970. We investigated how invasive R. rattus and native small mammal populations, as well as their fleas, have changed in recent decades. Over an 18-month period, a total of 2,959 small mammals were captured, sampled, and examined for fleas, resulting in the identification of 20 small mammal taxa that were hosts to 5,109 fleas (nine species). Over three-fourths (75.8%) of captured mammals belonged to four taxa: R. rattus, which predominated inside huts, …

Rnai: Applications In Vertebrate Pest Management, Katherine Horak

United States Department of Agriculture Wildlife Services: Staff Publications

Sequence-directed inhibition of protein synthesis by RNAi has potential as a means to control pest wildlife. Species specific by design, RNAi reduces impacts on nontarget species and the environment. Additional research advancing the field of RNAi-based management of vertebrate pest wildlife is timely.

Despite the potential diverse applications of RNAi technology in vertebrates (fertility control, invasive species eradication, and pest species control to protect human health and agriculture), little progress has been made in applying RNAi to these classes of animals. A single proof-of-concept study using RNAi to control sea lampreys combined with recent advances in RNAi delivery have opened …

Avian Influenza A Virus Associations In Wild, Terrestrial Mammals: A Review Of Potential Synanthropic Vectors To Poultry Facilities, J. Jeffrey Root, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

The potential role of wild mammals in the epidemiology of influenza A viruses (IAVs) at the farm-side level has gained increasing consideration over the past two decades. In some instances, select mammals may be more likely to visit riparian areas (both close and distant to farms) as well as poultry farms, as compared to traditional reservoir hosts, such as waterfowl. Of significance, many mammalian species can successfully replicate and shed multiple avian IAVs to high titers without prior virus adaptation and often can shed virus in greater quantities than synanthropic avian species. Within this review, we summarize and discuss the …

Improving The Accessibility And Transferability Of Machine Learning Algorithms For Identification Of Animals In Camera Trap Images: Mlwic2, Michael A. Tabak, Mohammad S. Norouzzadeh, David W. Wolfson, Erica J. Newton, Raoul K. Boughton, Jacob S. Ivan, Eric Odell, Eric S. Newkirk, Reesa Y. Conrey, Jennifer Stenglein, Fabiola Iannarilli, John Erb, Ryan K. Brook, Amy J. Davis, Jesse Lewis, Daniel P. Walsh, James C. Beasley, Kurt C. Vercauteren, Jeff Clune, Ryan S. Miller

United States Department of Agriculture Wildlife Services: Staff Publications

Motion-activated wildlife cameras (or “camera traps”) are frequently used to remotely and noninvasively observe animals. The vast number of images collected from camera trap projects has prompted some biologists to employ machine learning algorithms to automatically recognize species in these images, or at least filter-out images that do not contain animals. These approaches are often limited by model transferability, as a model trained to recognize species from one location might not work as well for the same species in different locations. Furthermore, these methods often require advanced computational skills, making them inaccessible to many biologists. We used 3 million camera …

Tissue Tropisms Of Avian Influenza A Viruses Affect Their Spillovers From Wild Birds To Pigs, Xiaojian Zhang, Fred L. Cunningham, Lei Li, Katie Hanson-Dorr, Liyuan Liu, Kaitlyn Waters, Minhui Guan, Alicia K. Olivier, Brandon S. Schmit, Jacqueline M. Nolting, Andrew S. Bowman, Mia Kim Torchetti, Thomas J. Deliberto, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Wild aquatic birds maintain a large, genetically diverse pool of influenza A viruses (IAVs), which can be transmitted to lower mammals and, ultimately, humans. Through phenotypic analyses of viral replication efficiency, only a small set of avian IAVs were found to replicate well in epithelial cells of the swine upper respiratory tract, and these viruses were shown to infect and cause virus shedding in pigs. Such a phenotypic trait of the viral replication efficiency appears to emerge randomly and is distributed among IAVs across multiple avian species and geographic and temporal orders. It is not determined by receptor binding preference …

Using Bioenergetics And Radar-Derived Bird Abundance To Assess The Impact Of A Blackbird Roost On Seasonal Sunflower Damage, Bonnie A. Clark, Page E. Klug, Phillip M. Stepanian, Jeffrey F. Kelly

United States Department of Agriculture Wildlife Services: Staff Publications

Methods aimed at reducing avian damage to agricultural crops are routinely implemented in situations where efficacy can be assessed by quantifying blackbird (Icteridae) abundance relative to environmental variables and extrapolating to ensuing crop damage. Concomitantly, Weather Surveillance Radar (WSR) data may have potential to enhance crop damage mitigation through improved monitoring of nuisance wildlife populations. We used WSR to derive daily abundance estimates of blackbirds at a fall roost in North Dakota, USA from 2012 to 2019. We integrated these estimates with previously developed bioenergeticseconomic models to estimate local sunflower (Helianthus annuus) damage. The greatest losses usually occurred during …

Historic And Contemporary Use Of Catfish Aquaculture By Piscivorous Birds In The Mississippi Delta, Paul C. Burr, Jimmy L. Avery, Garrett M. Street, Bronson K. Strickland, Brian S. Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Piscivorous birds are the primary source of catfish (Ictalurus spp.) depredation at aquaculture facilities in northwestern Mississippi. Of particular concern is the Double-crested Cormorant (Phalacrocorax auritus), which can cost aquaculture producers millions of dollars annually through the depredation of cultured fish. Historical research conducted in the early 2000s estimated cormorant use of aquaculture ponds in the region, but aquaculture area has decreased by more than 70% since those estimates were made. With less aquaculture available, we predicted cormorant densities on aquaculture would be greater today than historically. Applying a similar methodology as in historical studies, we used …

Invasive Species Control And Resolution Of Wildlife Damage Conflicts: A Framework For Chemical And Genetically Based Management Methods, Larry Clark, John Eisemann, John Godwin, Katherine Horak, Kevin Oh, Jeanette R. O'Hare, Antoinette J. Piaggio, Kim M. Pepin, Emily W. Ruell

United States Department of Agriculture Wildlife Services: Staff Publications

Vertebrate wildlife damage management relates to developing and employing methods to mitigate against damage caused by wildlife in the areas of food production, property damage, and animal or human health and safety. Of the many management tools available, chemical methods (e.g., toxicants) draw the most attention owing to issues related to environmental burden, species specificity, and humaneness. Research and development focusing on RNA interference and gene drives may be able to address the technical aspects of performance goals. However, there remain many questions about regulation, environmental risk, and societal acceptance for these emerging biological technologies. Here we focus on the …

Repellent Surface Applications For Pest Birds, Shelagh T. Deliberto, James C. Carlson, Hailey E. Mclean, Caroline S. Olson, Scott J. Werner

United States Department of Agriculture Wildlife Services: Staff Publications

Common pest birds in the United States include the non-native European starling (Sturnus vulgaris), house sparrow (Passer domesticus), and the pigeon (Columba livia domestica), as well as native birds including Canada geese (Branta canadensis) and gull species (Laridae). Large concentrations of pest birds can create human health hazards and monetary losses due to consumption of crops, depredation, and fecal contamination and accumulation. Fecal contamination hazards include the potential spread of zoonotic diseases including antimicrobial-resistant zoonoses and human injury due to the accumulation of fecal material on walking surfaces. Additionally, fecal accumulation causes …

Black Vulture Conflict And Management In The United States: Damage Trends, Management Overview, And Research Needs, Bryan M. Kluever, Morgan Pfeiffer, Scott C. Barras, Brett Dunlap, Lee A. Humberg

United States Department of Agriculture Wildlife Services: Staff Publications

Contrary to rapid declines of many vulture (Accipitridae, Cathartidea) species worldwide, black vulture (Coragyps atratus) populations are increasing and expanding their range in North America. Vultures exhibit complex behaviors and can adapt to any human-dominated landscape or land use. These traits, combined with population growth and range expansion, have contributed to increased human–vulture conflicts. Our goal was to summarize the current status and trends in human–black vulture conflicts (hereafter human– vulture conflicts), review available management strategies, identify knowledge gaps, and provide recommendations to enhance management and understanding of this species and the associated conflicts. We found human–vulture conflicts …

Review Of Ospreys: The Revival Of A Global Raptor, By Alan F. Poole. 2019., Lisa Fields, Brian E. Washburn

United States Department of Agriculture Wildlife Services: Staff Publications

For the past three decades, Alan Poole’s (1989) Ospreys: A Natural and Unnatural History has provided a solid foundation for those seeking a primer on the basic biology and ecology of Ospreys (Pandion haliaetus). Poole’s summary of knowledge about Ospreys was written at a time when these amazing birds were recovering from their DDTinduced population crash, but this important book has long been out of print. In the intervening decades, much has been learned regarding Ospreys. In his new book, Ospreys: The Revival of a Global Raptor, Poole guides the reader through a tour of Ospreys’ worldwide distribution and population …

Population Increases Of Large Birds In North America Pose Challenges For Aviation Safety, Richard A. Dolbeer

United States Department of Agriculture Wildlife Services: Staff Publications

There is a strong correlation between bird mass and the likelihood of aircraft damage during a bird–aircraft collision. Thus, the U.S. Federal Aviation Administration (FAA) has established airworthiness standards related to bird mass for engines, airframes, and windshields. Most standards use large (1.8 kg) and medium (1.1 kg) birds as benchmarks (the empennage and certain large turbofan engines use a 3.6-kg bird). There are 20 large (≥1.8 kg) and 16 medium (1.1–1.7 kg) bird species in North America with ≥20 strikes reported for civil aircraft (FAA National Wildlife Strike Database), 1990 to 2018. I analyzed the population changes of these …

Coproid Predicts The Source Of Coprolites And Paleofeces Using Microbiome Composition And Host Dna Content, Maxime Borry, Bryan Cordova, Angela Perri, Marsha Wibowo, Tanvi Prasad Honap, Jada Ko, Kate Britton, Linus Girdland-Flink, Robert C. Power, Ingelise Stuijts, Domingo C. Salazar-García, Courtney Hofman, Richard Hagan, Thérèse Samdapawindé Kagoné, Nicolas Meda, Helene Carabin, David Jacobson, Karl Reinhard, Cecil Lewis, Aleksandar Kostic, Choongwon Jeong, Alexander Herbig, Alexander Hübner, Christina Warinner

Karl Reinhard Publications

Shotgun metagenomics applied to archaeological feces (paleofeces) can bring new insights into the composition and functions of human and animal gut microbiota from the past. However, paleofeces often undergo physical distortions in archaeological sediments, making their source species difficult to identify on the basis of fecal morphology or microscopic features alone. Here we present a reproducible and scalable pipeline using both host and microbial DNA to infer the host source of fecal material. We apply this pipeline to newly sequenced archaeological specimens and show that we are able to distinguish morphologically similar human and canine paleofeces, as well as non-fecal …

Diet Analysis Reveals Pre-Historic Meals Among The Loma San Gabriel At La Cueva De Los Muertos Chiquitos, Rio Zape, Mexico (600–800 Ce), Elisa Pucu, Julia Russ, Karl Reinhard

Karl Reinhard Publications

Coprolites have been a source of study for archeologists due to several reasons: they not only provide information on the life and nutritional habits of ancient individuals but also on their health. In this paper, we processed 10 coprolites collected at La Cueva de Los Muertos Chiquitos (600–800 CE), Rio Zape, Mexico, with acetolysis solution for pollen analysis. The number of pollen grains/gram of each coprolite sample was quantified along with the macroscopic remains of these samples. The main food item ingested by the population was maize, followed by Agave. Squash blossoms were also part of their food source …

Pinworm Research In The Southwest Usa: Five Decades Of Methodological And Theoretical Development And The Epidemiological Approach, Morgana Camacho, Karl Reinhard

Karl Reinhard Publications

Pinworms infected Ancestral Pueblo populations since early periods of occupation on the Colorado Plateau. The high prevalence of pinworm found in these populations was correlated with the habitation style developments through time. However, in previous studies, Turkey Pen Cave, an early occupation site, and Salmon Ruins, a late occupation site, exhibited prevalences that were anomalously low, suggesting that these sites were outliers. Alternatively, it is possible that the previous quantification method was not successful in detecting the real prevalence and eggs per gram, which led to inexact interpretations. The aims of this study were to verify if previous pinworm prevalences …

Humans, Wildlife, And Our Environment: One Health Is The Common Link, Terry A. Messmer

Human–Wildlife Interactions

One Health has become more important in recent years because interactions between people, animals, plants, and our environment have dramatically changed. This Back Page article discusses One Health during the COVID-19 pandemic.

Analysis Of Changes In Genes That Lead To Antibiotic Resistance In Klebsiella Pneumoniae 43816, Jasmine R. Anderson

UNF Graduate Theses and Dissertations

Klebsiella pneumoniae is a Gram-negative bacterium responsible for nosocomial infections such as blood stream infections, meningitis, and septicemia. Clinical isolates confirm K. pneumoniae is increasingly antibiotic resistant (AR) making treatment more difficult. While bacterial exposure to antimicrobial substances is a known cause of the AR phenotype, there still exists gaps in knowledge about the genetic changes responsible for this fitness change. The laboratory Klebsiella pneumoniae 43816 (ATCC 43816) was exposed to sublethal concentrations of Cephalothin for a 14-day period. At the end of the experimental treatment, the adapted population acquired clinical antibiotic resistance along with phenotypic and morphological changes. The …

Sdg 3: Good Health And Well-Being - Framing Targets To Maximise Co-Benefits For Forests And People, Rosemary A. Mcfarlane, John Barry, Guéladio Cissé, Maya Gislason, Marta Gruca, Kerryn Higgs, Pierre Horwitz, Giang Nguyen, Jane O'Sullivan, Subhashis Sahu, Colin D. Butler

Research outputs 2014 to 2021

Key Points: The achievement of SDG 3 depends on many other SDGs; some SDGs are logically inconsistent, especially in the attempt to increase conventionally defined GDP while preserving natural capital. Any short-term gains for human health from further forest conversion (e.g. food production) creates short- and long-term, direct and indirect health risks for humans, as well as for other biota. Failure to ensure universal access to sexual and reproductive healthcare services (including family planning) will increase pressure on forests at local, regional and global scales. The burning and clearing of forests cause significant harm to health via impaired quality of …

One Welfare, The Role Of Health Professionals, And Climate Change, Anne Fawcett

Animal Sentience

Wiebers & Feigin argue that the COVID-19 crisis is a call for humanity to rethink our relationships with animals and the environment. This One Welfare approach has implications for the role of health care professionals and demands that we address climate change.

Wildlife Health Systems, Lee Skerratt

Animal Sentience

Wildlife health systems aim to ensure that all animal life is healthy and resilient. They protect biodiversity and ecosystem services and ensure that the risk of spillover of pathogens is mitigated. These systems are flexible, multidisciplinary and cross-sectorial. They can manage a variety of threats to life that arise in different communities and cultures. Very small investments are required to ensure that wildlife health systems function effectively.

Covid-19, Evolution, Brains And Psychology, Frederick Toates

Animal Sentience

Attention needs to be directed to the processes that control behavior in humans and the adaptive problems that they solved in our early evolutionary environment. The evolutionary mismatch between the current environment and the human brain can yield important insights into the problems that beset us in the context of environmental degradation and nonhuman animal welfare.

Re-Engage With The World For Global Health And Animal Welfare, Bradley J. Bergstrom

Animal Sentience

Wiebers & Feigin (2020) make a strong argument for measures that would limit future zoonoses, such as the COVID-19 pandemic, by closing live-animal markets, changing our habits of food consumption and production, and reducing habitat destruction. These would help human health, animal welfare, and conservation of at-risk wildlife all at the same time. China’s command-and-control government can accomplish some of these things by edict, but slower-to-act western democracies play a surprisingly large role in these global problems by the power of their consumerism, including the illicit wildlife trade. We citizens need to insist that our government use all of its …

Asian Elephant Rescue, Rehabilitation And Rewilding, Liv Baker, Rebecca Winkler

Animal Sentience

Thailand has fewer than 10,000 elephants left. More of them are living in captivity to serve the tourist industry under grim conditions than are living free in what is left of their wild habitat. Conservation efforts need to be focused on all surviving members of the species, captive and free, but they need to take into account the inextricable entanglement of human and nonhuman animal lives in Thailand today. There is an opportunity for rescuing, rehabilitating and reintroducing captive elephants to the wild with the help of the traditional expertise of a mahout culture that has been elephant-keeping for centuries. …

Just Preservation, Trusteeship And Multispecies Justice, Francisco J. Santiago-Ávila, Adrian Treves, William Lynn

Animal Sentience

We are grateful to all the commentators who engaged with our target article. Some commentators have offered important insights into our proposed design and methods for legally intervening on behalf of futurity. Others have focused on theoretical considerations central to our proposal for multispecies justice and trusteeship. All have inspired modifications and further elaboration of our initial proposal. In this Response, we engage with the commentaries, integrating their suggestions, striving for convergence and complementarity, but also discussing points of divergence with our proposed framework where necessary. There is substantial overlap in the points of view of the three co-authors, but …

A Model For The Prediction Of Antimicrobial Resistance In Escherichia Coli Based On A Comparative Evaluation Of Fatty Acid Profiles, Randal S. Stahl, Bledar Bisha, Sebabrata Mahapatra, Jeffrey C. Chandler

United States Department of Agriculture Wildlife Services: Staff Publications

Antimicrobial resistance is a threat to agricultural production and public health. In this proof-of-concept study, we investigated predicting antimicrobial sensitive/resistant (S/R) phenotypes and host sources of Escherichia coli (n = 128) based on differential fatty acid abundance. Myristic (14:0), pentadecanoic acid (15:0), palmitic (16:0), elaidic (18:19) and steric acid (18:0) were significantly different (α = 0.05) using a two-way ANOVA for predicting nalidixic acid, ciprofloxacin, aztreonam, cefatoxime, and ceftazidime S/R phenotypes. Additionally, analyses of palmitoleic (16:1), palmitic acid (16:0), methyl palmitate (i-17:0), and cis-9,10-methyleneoctadecanoic acid (19:0Δ) showed these markers were significantly different (α = 0.05) between isolates obtained from cattle …

Reconstructing Energy Flow Through Modern And Historical Marine Communities: Insights From Amino Acid Isotope Analysis, Emma A. Elliott Smith

Biology ETDs

The fundamental currency of life is energy. Organisms need energy to grow, to survive and to reproduce. Understanding the acquisition of energy by consumers is thus a foundational aspect of biological research. This is especially important in the modern era, as impacts of ongoing anthropogenic effects will be mediated or amplified through food webs. Here, I explore how isotopic analysis of individual amino acids – a technique new to ecological studies – can be used to trace energy flow through animal communities in modern and ancient time periods. In particular, I focus on kelp forest food webs, which are nearshore …