Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Ecology and Evolutionary Biology

Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 4501 - 4530 of 12826

Full-Text Articles in Entire DC Network

A Male-Female Genotype-By-Genotype Interaction Mediates The Effect Of Mating On Female Immunity In Decorated Crickets, Kylie J. Hampton Dec 2019

A Male-Female Genotype-By-Genotype Interaction Mediates The Effect Of Mating On Female Immunity In Decorated Crickets, Kylie J. Hampton

Theses and Dissertations

Sexually antagonistic coevolution should lead to the rapid divergence of male and female genotypes related to the effects of ejaculatory substances on female physiology. Hence, the outcome of mating should depend on an interaction between male and female genotypes. Although mating has been shown to influence female immune responses in diverse insect taxa, a male-female genotype-by-genotype effect on female immunity post-mating remains largely unexplored. Here, we investigate both the effects of mating on female immunity and the potential for a male-by-female genotype interaction on the form and magnitude of this response in decorated crickets. Females from three distinct genotypic backgrounds …


Springs Of The Arabian Peninsula: Historical Trends, Current Status And Human Impact In Saudi Arabia, Oman And Jordan, Kamal M. Aljohani Dec 2019

Springs Of The Arabian Peninsula: Historical Trends, Current Status And Human Impact In Saudi Arabia, Oman And Jordan, Kamal M. Aljohani

USF Tampa Graduate Theses and Dissertations

This research was conducted to update previous studies of the springs of the Arabian Peninsula (Saudi Arabia, Oman and Jordan). Approach: 76 springs were surveyed in between March and July 2018: 15 Saudi Arabia, 41 Oman and 20 Jordan to ascertain the current status, water quality and zooplankton benthos and fish communities. Investigation of the changes of biota and chemical parameters downstream from the springs source in Saudi Arabia. Results: Mercury exceeded the WHO and USEPA maximum contaminant level (MCL) in 63 of 76 springs, Aluminum exceeded MCL in 8 of 76 springs, and both were the common dissolved heavy …


Scorpions Of The Horn Of Africa (Arachnida, Scorpiones). Part Xxii. Two New Species Of Neobuthus From Somaliland (Buthidae), František Kovařík Dec 2019

Scorpions Of The Horn Of Africa (Arachnida, Scorpiones). Part Xxii. Two New Species Of Neobuthus From Somaliland (Buthidae), František Kovařík

Euscorpius

New information about taxonomy and distribution of the genus Neobuthus Hirst, 1911 is presented, based on material recently collected in Somaliland. N. awashensis Kovařík & Lowe, 2012 is reported from Somaliland for the first time. Two new species are described, N. haeckeli sp. n. and N. solegladi sp. n. An updated distribution map of the genus Neobuthus is provided.


Genus Olivensa Lane, 1965 In French Guiana, And Description Of A New Species (Coleoptera: Cerambycidae: Lamiinae), Pierre-Henri Dalens, Frédéric Robin Dec 2019

Genus Olivensa Lane, 1965 In French Guiana, And Description Of A New Species (Coleoptera: Cerambycidae: Lamiinae), Pierre-Henri Dalens, Frédéric Robin

Insecta Mundi

Olivensa sonzognii Dalens and Robin, sp. nov. (Coleoptera: Cerambycidae: Lamiinae) is described from French Guiana and compared to other local species. A new key for the genus Olivensa Lane, 1965 is proposed.


The Effects Of Urbanization On Bee Communities Depends On Floral Resource Availability And Bee Functional Traits., Caleb J Wilson, Mary A Jamieson Dec 2019

The Effects Of Urbanization On Bee Communities Depends On Floral Resource Availability And Bee Functional Traits., Caleb J Wilson, Mary A Jamieson

Entomology Faculty Publications

Wild bees are important pollinators in many ecosystems threatened by anthropogenic disturbance. Urban development can reduce and degrade natural habitat for bees and other pollinators. However, some researchers suggest that cities could also provide refuge for bees, given that agricultural intensification may pose a greater risk. In this study, we surveyed bee communities at 15 farms and gardens across an urban-rural gradient in southeastern Michigan, USA to evaluate the effect of urbanization on bees. We examined how floral resources, bee functional traits, temperature, farm size, and the spatial scale of analysis influence bee response to urbanization. We found that urbanization …


Evolution - #2: Evolutionary Evidence And Inferences, Jessica Amacher, Sowmya Anjur, Donald Dosch, Sarah O'Leary-Driscoll, Crystal Randall Dec 2019

Evolution - #2: Evolutionary Evidence And Inferences, Jessica Amacher, Sowmya Anjur, Donald Dosch, Sarah O'Leary-Driscoll, Crystal Randall

Unit 1: Evolution

The theory of evolution articulates elegantly a series of inferences from a vast collection of observations and evidence. We present some of those evidences here to stimulate in our students the sorts of scientific reasoning necessary to understand the presence and origin of diversity of life on the planet.


Evolution - #1: History Of Life, Jessica Amacher, Sowmya Anjur, Donald Dosch, Sarah O'Leary-Driscoll, Crystal Randall Dec 2019

Evolution - #1: History Of Life, Jessica Amacher, Sowmya Anjur, Donald Dosch, Sarah O'Leary-Driscoll, Crystal Randall

Unit 1: Evolution

From the perspective of a biologist, the history of life on the Earth is a recurring story. Although details may be different, what happens today has precedence in the occurrences of the past. Two underlying ideas are vital for students to understand a modern conception of the theory of evolution. First, over long periods of time, and due to natural events, the environment of the Earth has changed, sometimes dramatically. Second, each environmental change has created more favorable conditions for some species to live and reproduce, while producing less favorable conditions for others. This first lesson in our unit on …


Evolution - #6: Selection Simulation, Jessica Amacher, Sowmya Anjur, Donald Dosch, Sarah O'Leary-Driscoll, Crystal Randall Dec 2019

Evolution - #6: Selection Simulation, Jessica Amacher, Sowmya Anjur, Donald Dosch, Sarah O'Leary-Driscoll, Crystal Randall

Unit 1: Evolution

A modern understanding of the theory of evolution revolves around the selection of certain types within a population that over long time periods eventually leads to change of the species. This descent with modification is modeled with this activity, and leads to a subsequent lesson that has students reading about research into examples of speciation.


Evolution - #5: Globin Gene Evolution, Jessica Amacher, Sowmya Anjur, Donald Dosch, Sarah O'Leary-Driscoll, Crystal Randall Dec 2019

Evolution - #5: Globin Gene Evolution, Jessica Amacher, Sowmya Anjur, Donald Dosch, Sarah O'Leary-Driscoll, Crystal Randall

Unit 1: Evolution

For this lesson, students are guided through the steps necessary to compare nucleotide and amino acid sequences available at the National Center for Biotechnology Information that is maintained by the National Institutes of Health. The lesson starts with an examination of the alpha-globin gene. Alpha-globin is one of the proteins that constitutes hemoglobin. Student look at the various sequences of this gene that have been deposited at NCBI with a goal of determining alternative forms of the gene. The lesson then broadens the scope of comparison to include members of the globin protein family. Students create a phylogenetic tree that …


Index To Volume 87 Dec 2019

Index To Volume 87

Nebraska Bird Review

14 pages: Akers, Danny 108 to Zeglen, Nancy 45


2019 Hitchcock Raptor Banding Project In Perspective, Jerry Toll Dec 2019

2019 Hitchcock Raptor Banding Project In Perspective, Jerry Toll

Nebraska Bird Review

Hitchcock Nature Center in Pottawattamie County, north of Crescent, Iowa, is home to the Hitchcock HawkWatch and Raptor Banding Project. The Nature Center is in the heart of the Loess Hills which, together with the flat expanse of the Missouri River Valley to the west, provides the perfect conditions for creating the updraft that aids migrating birds. The Hawkwatch began in 1992, and every fall from September through December, birds-of-prey enthusiasts gather on the 45-foot observation tower to watch and count thousands of eagles, hawks, and other raptors as they migrate south. Every year since 2007, Sandy Reinken and I …


The Nebraska Bird Review, Volume 87 December 2019 Number 4 Dec 2019

The Nebraska Bird Review, Volume 87 December 2019 Number 4

Nebraska Bird Review

Fall Field Report, August - November 2019, by W. Ross Silcock …118-138

2019 Hitchcock Raptor Banding Project in Perspective, by Jerry Toll …138-147

Imperial Fall Field Days, Sept 13-15, 2019 …148-152

Index to Volume 87 …153-166

Subscription and Organization Information …167


Serological Survey And Pathogen Exposure Of Adult Female White-Tailed Deer In The Western Dakotas, Katherine L. Moratz, Bailey S. Gullikson, Eric S. Michel, Daniel M. Grove, Jonathan A. Jenks, William Jensen Dec 2019

Serological Survey And Pathogen Exposure Of Adult Female White-Tailed Deer In The Western Dakotas, Katherine L. Moratz, Bailey S. Gullikson, Eric S. Michel, Daniel M. Grove, Jonathan A. Jenks, William Jensen

The Prairie Naturalist

Establishing baseline values for pathogen exposure and nutritional indices is necessary to monitor population health. However, little is known about white-tailed deer (Odocoileus virginianus) pathogen exposure and nutritional condition in the Northern Great Plains. Our objective was to assess pathogen exposure and establish nutritional indices for female white-tailed deer in Dunn and Grant counties, North Dakota and Perkins County, South Dakota. During 2014, we collected blood serum from 150 adult female white-tailed deer. Pathogens with the highest antibody prevalence included West Nile Virus (WNV; 85%), epizootic hemorrhagic disease (48%), and malignant catarrhal fever (32%). Serum values for creatine …


Imperial Fall Field Days, Janis Paseka Dec 2019

Imperial Fall Field Days, Janis Paseka

Nebraska Bird Review

The 2019 NOU Fall Field Days were held in Imperial, Chase County, on September 13 - 15, with 59 registered. Field trips were led by Mark Brogie, Bill Flack, and T. J. Walker to the Wauneta cemetery, Frenchman WMA, Hayes Center WMA, Enders Reservoir WMA and SRA, Champion Mill Lake, Swinging Bridge Arboretum, Church Grove Recreation Area, Rock Creek Lake SRA and Fish Hatchery, and Swanson Reservoir SRA and WMA. Among the highlights were Osprey, which were seen at several locations, Sabine’s Gull, Ferruginous Hawk, Burrowing Owl, Red-naped Sapsucker, Merlin, and Prairie Falcon. Anita Breckbill, Doctor of Musical Arts, head …


Bats Of The Loess Hills Ecoregion Of Southeast Nebraska, Virgil Brack Jr, Dale W. Sparks, Darwin C. Brack Dec 2019

Bats Of The Loess Hills Ecoregion Of Southeast Nebraska, Virgil Brack Jr, Dale W. Sparks, Darwin C. Brack

The Prairie Naturalist

We surveyed bats at 49 sites in the Loess Hills Ecoregion of southeastern Nebraska, along the western edge of the eastern forest biome in eastern Richardson, Nemaha, and Otoe counties. We completed this study shortly before the northern long-eared bat (Myotis septentrionalis) was listed by the United States Fish and Wildlife Service under the Endangered Species Act. The expectation of listing, along with potential presence of the endangered Indiana bat (Myotis sodalis), motivated the study. We captured 183 bats of five species: eastern red bat (Lasiurus borealis) (n = 103; 56 %), big brown …


A Survey Of Earthworm Communities Across Areas Of Varying Land Management In Northwest Arkansas, Alyssa M. Ferri Dec 2019

A Survey Of Earthworm Communities Across Areas Of Varying Land Management In Northwest Arkansas, Alyssa M. Ferri

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

Earthworms are an integral component of the soil ecosystem, impacting factors including soil formation, maintenance of soil structure, and nutrient recycling. Earthworm abundances and distributions are related to abiotic soil properties and can influence the community structure. The goal of this study was to survey earthworm populations across areas of different land management histories and soil characteristics to determine if a relationship between earthworm density and land management characteristics was present. Earthworm and soil samples were collected at irregular temporal intervals from September 2017 to March 2018. In total, five sites were sampled, three at the Botanical Garden of the …


Release Of Captive-Raised Eastern Hellbenders (Cryptobranchus Alleganiensis) To Test The Success Of A Chytrid Vaccine And New Cage Design, Megan C. Kocher Dec 2019

Release Of Captive-Raised Eastern Hellbenders (Cryptobranchus Alleganiensis) To Test The Success Of A Chytrid Vaccine And New Cage Design, Megan C. Kocher

Biology Theses

Researchers and managers commonly apply captive-raising and reintroductions of animals to offset losses due to worldwide amphibian declines. Recent declines in the Eastern hellbender (Cryptobranchus alleganiensis alleganiensis) have resulted in several reintroductions that have had little success. There is evidence that chytridiomycosis (chytrid), a disease caused by the fungus Batrachochytrium dendrobatidis (Bd), may negatively affect hellbenders post-release. Further, excessive post-release movement may result in movement away from suitable habitat and increased risk of predation which can have negative effects on the survival of released hellbenders. Caging captive-raised animals can be effective for limiting excessive post-release movement. …


Movements And Behaviors Of Resident And Translocated Northern Map Turtles (Graptemys Geographica) In The Upper Niagara River With Artificial Basking/Nesting Platforms As A Management Strategy, Chelsea L. Moore Dec 2019

Movements And Behaviors Of Resident And Translocated Northern Map Turtles (Graptemys Geographica) In The Upper Niagara River With Artificial Basking/Nesting Platforms As A Management Strategy, Chelsea L. Moore

Biology Theses

Urbanization and degradation of riparian zones has contributed to the decline of fish and wildlife populations throughout the world. Northern Map Turtles (Graptemys geographica) in the upper Niagara River face similar declines due to shoreline development and the concurrent loss of backwater habitats and terrestrial nesting sites. A project was initiated in which basking/nesting platforms were created, and Northern Map Turtles from a Lake Erie population were translocated into the river. Resident and translocated turtles were tracked using biotelemetry and their habitat use, behaviors, and swimming paths were compared. Translocated turtles exhibited homing behavior and had longer home …


Use Of Uav Imagery And Nutrient Analyses For Estimation Of The Spatial And Temporal Contributions Of Cattle Dung To Nutrient Cycling In Grazed Ecosystems, Amanda E. Shine Dec 2019

Use Of Uav Imagery And Nutrient Analyses For Estimation Of The Spatial And Temporal Contributions Of Cattle Dung To Nutrient Cycling In Grazed Ecosystems, Amanda E. Shine

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nutrient inputs from cattle dung are crucial drivers of nutrient cycling processes in grazed ecosystems. These inputs are important both spatially and temporally and are affected by variables such as grazing strategy, water location, and the nutritional profile of forage being grazed. Past research has attempted to map dung deposition patterns in order to more accurately estimate nutrient input, but the large spatial extent of a typical pasture and the tedious nature of identifying and mapping individual dung pats has prohibited the development of a time- and cost-effective methodology. The first objective of this research was to develop and validate …


Observable Persistent Effects Of Habitat Management Efforts In The Ozark Highlands After 10 Years, Maxwell Carnes-Mason Dec 2019

Observable Persistent Effects Of Habitat Management Efforts In The Ozark Highlands After 10 Years, Maxwell Carnes-Mason

Graduate Theses and Dissertations

I investigated the lasting impacts of a management plan designed to improve oak regeneration and benefit wildlife in the Ozark Highlands in Madison, Co., AR. To assess the efficacy of the management plan, I used variables relevant to the success and establishment of oak trees. Controlled burns and selective logging were used to thin the canopy, increase ground level productivity, and increase the abundance of small mammals. I used measurements of overstory and understory densities, light availability, and the density of mice in the genus Peromyscus across time to look at the lasting impacts of management. Different treatment plots were …


Species Richness And Ecological Diversity Of Myxomycetes And Myxomycete-Like Organisms In The Tropical Forests Of Brazil, Isadora Lima Coelho Dec 2019

Species Richness And Ecological Diversity Of Myxomycetes And Myxomycete-Like Organisms In The Tropical Forests Of Brazil, Isadora Lima Coelho

Graduate Theses and Dissertations

Tropical rain forests cover less than two percent of Earth's surface, yet they sustain the greatest diversity of living organisms on the planet. Tropical rain forests cover nearly 73% of the Brazilian territory and besides harboring some of the most biodiverse ecosystems on the planet, this vast area also houses about 83% of the Brazilian population. Approximately 175 million people live in urban and rural areas with fragments of coverage of these biomes which contributes to the loss of biodiversity that rapidly increases over the years. Furthermore, the majority of the taxonomic and ecological efforts to describe and protect the …


Ecology And Conservation Of Shrubland Bird Communities In The Eastern Ghats Of Indi, Anant Deshwal Dec 2019

Ecology And Conservation Of Shrubland Bird Communities In The Eastern Ghats Of Indi, Anant Deshwal

Graduate Theses and Dissertations

Anthropogenic disturbance, in its multiple facets represents a major threat to biodiversity and habitat quality. Consequently, extensive research is guided towards understanding anthropogenic disturbance and their effects on wildlife for development of wildlife management plans. However, for development of effective wildlife management plans it is imperative that we understand the habitat use and preference by local fauna along with effects of anthropogenic presence. In this dissertation, I studied the habitat usage and preferences of Shrubland birds in the Eastern Ghats of India during the pre-monsoon and post monsoon seasons. Eastern Ghats show a marked difference from pre-monsoon season to post-monsoon …


Eastern Collared Lizard (Crotaphytus Collaris) Population Declines In Ozark Landscapes: An Assessment Of Environmental Constraints., Casey L. Brewster Dec 2019

Eastern Collared Lizard (Crotaphytus Collaris) Population Declines In Ozark Landscapes: An Assessment Of Environmental Constraints., Casey L. Brewster

Graduate Theses and Dissertations

The Eastern Collared Lizard (Crotaphytus collaris) has experienced extensive population declines over the past half century in the Ozark Mountains. Previous research suggests that glade habitat degradation through woody vegetation encroachment is an important factor resulting in population declines. In this dissertation I used information on time-energy budgets to investigate the link between habitat degradation and shifts in life history traits likely resulting in population declines of Eastern Collared Lizards (Crotaphytus collaris) in the Ozarks. In chapter one, I addressed the influence of dense woody vegetation encroachment on age-specific growth, body size, body condition and reproduction of C. collaris in …


Snowshoe Hare (Lepus Americanus) Abundance In Relation To Habitat And Predator Assemblage Across The Apostle Islands, Jarod Reibel Dec 2019

Snowshoe Hare (Lepus Americanus) Abundance In Relation To Habitat And Predator Assemblage Across The Apostle Islands, Jarod Reibel

Masters Theses

Snowshoe hares (Lepus americanus) are a climate sensitive species that have a southern range boundary moving northward. Snowshoe hares are found on the Apostle Islands, Wisconsin which are near their southern boundary and differ by island in vegetative and carnivore communities. The archipelago serves as a natural laboratory to assess how top-down and bottomup forces interact and impact snowshoe hare populations. The objectives of this study were to determine the influence of vegetative characteristics, specifically visual obstruction, and the presence of predators on snowshoe hare abundances across the Apostle Islands. We conducted fecal pellet surveys to estimate hare abundance, measured …


The Implementation Of Qpcr Beach Monitoring Methods: Analysis Of A Multi-Lab Validation Study And The Role Of Environmental Parameters On A Comparison Of Colilert And Qpcr Methods, Molly J. Lane Dec 2019

The Implementation Of Qpcr Beach Monitoring Methods: Analysis Of A Multi-Lab Validation Study And The Role Of Environmental Parameters On A Comparison Of Colilert And Qpcr Methods, Molly J. Lane

Masters Theses

Public beaches are routinely tested for potentially pathogenic bacteria to protect beachgoers from possible illness. An EPA approved method, Colilert™, used for testing E. coli in recreational water requires 18 – 22 hours before a result is reported but, recreators have already contacted unsafe water before the beach is closed. My study focused on a U.S. EPA proposed qPCR method (Draft Method C) to quantify E. coli in recreational waters that can provide same-day results. In Chapter 2, I examined the calibration procedure used to validate Draft Method C and compared standard curve intercept and slope estimates calculated with a …


Impacts Of Shoreline Restoration And Source Of Nutrient Enrichment On Macrophytes And Epiphytic Algal Communities, Paige Marie Kleindl Dec 2019

Impacts Of Shoreline Restoration And Source Of Nutrient Enrichment On Macrophytes And Epiphytic Algal Communities, Paige Marie Kleindl

Masters Theses

Macrophytes and their epiphytic algal communities are integral for optimizing littoral ecosystem functioning in lakes. Epiphytic algae’s placement on the plant’s surface can reduce light and nutrient availability (i.e., nitrogen and phosphorus) for the host macrophyte. Macrophyte and epiphytic algal proximity complicates these primary producer group interactions and responses to bioavailable nutrients in the water column or porewater. For example, epiphytic algae may have a competitive advantage over surface water nutrients compared to macrophytes, which may have a competitive advantage over porewater nutrients via root systems.

Muskegon Lake’s industrial history and designation as an Area of Concern prompted shoreline restoration, …


Habitat Use Of The Climate-Sensitive Snowshoe Hare (Lepus Americanus) In The Manistee National Forest In Michigan’S Lower Peninsula, Spencer D. West Dec 2019

Habitat Use Of The Climate-Sensitive Snowshoe Hare (Lepus Americanus) In The Manistee National Forest In Michigan’S Lower Peninsula, Spencer D. West

Masters Theses

Snowshoe hares (Lepus americanus) are a wide-ranging lagomorph that are important forest herbivores and a popular game species throughout their range. Across the southern boundary of their geographic range, snowshoe hares are experiencing population declines and possible extirpation due to increased predation pressure driven by climate change induced camouflage mismatch, competition for forage, degraded and fragmented habitat. One method of reversing the negative trends in snowshoe hare distribution is to increase and improve available hare habitat. A specific habitat analysis for local regions will most effectively advise managers how to target habitat management. I radio-collared 11 snowshoe hares in the …


Mammal Species Inventory Using Various Trapping Methods In Zone 4 Of Billy Barquedier National Park, Belize During Rainy Season, Mersady Redding Dec 2019

Mammal Species Inventory Using Various Trapping Methods In Zone 4 Of Billy Barquedier National Park, Belize During Rainy Season, Mersady Redding

Animal Science Undergraduate Honors Theses

Belize is a small country, but it is extremely ecologically diverse. Based on the few studies conducted in Belize, the abundance of mammals is low but diversity is high. Particular findings note the number and identity of species differed between four sites in the Maya Mountains of Belize, indicating that a data set from a single site is not representative of the Neotropical region. Insufficient data is available to estimate current species richness of many areas in Belize, including Billy Barquedier National Park (BBNP). The objective of this study was to explore trapping and documentation methods of terrestrial mammals in …


Freshwater Turtle Community Composition In Mined Land Strip Pit Lakes And The Effects Of Learned Trap Avoidance On Capture Rates Of Sternotherus Odoratus And Trachemys Scripta, Ethan Craig Hollender Dec 2019

Freshwater Turtle Community Composition In Mined Land Strip Pit Lakes And The Effects Of Learned Trap Avoidance On Capture Rates Of Sternotherus Odoratus And Trachemys Scripta, Ethan Craig Hollender

Graduate Theses/Dissertations

Turtles are among the most threatened groups of organisms on the planet and as such are in need of protected habitat where healthy communities can be maintained. The reclamation of land that was formerly the site of surface strip mining provides a matrix of reclaimed terrestrial landscapes rich with lakes and ponds that have the potential to function as such habitat. To determine the suitability of these habitats for turtle communities, I compared the turtle communities of strip pit lakes and natural lakes in southeastern Kansas. Of the seven species of aquatic turtle I encountered, Common Snapping Turtles (Chelydra …


Behavioral Aspects Of Chemoreception In Juvenile Cottonmouths (Agkistrodon Piscivorus), Chelsea E. Martin Dec 2019

Behavioral Aspects Of Chemoreception In Juvenile Cottonmouths (Agkistrodon Piscivorus), Chelsea E. Martin

Graduate Theses/Dissertations

For snakes, chemical recognition of predators, prey, and conspecifics has important ecological consequences. For example, detection of predator cues can reduce predation risk. Similarly, scent trailing of conspecifics to communal hibernacula can improve overwinter survival for neonates. I used y-maze choice trials to examine scent-trailing ability of 32 captive-born juvenile cottonmouths (Agkistrodon piscivorus) in two separate experiments. In conspecific trailing tests, subjects preferred to follow cues from their own mothers over a blank control cue, but also preferred to trail cues from unrelated adult females compared to cues from their own mothers. My results are consistent with previous …