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Disaster Plant Pathology: Smart Solutions For Threats To Global Plant Health From Natural And Human-Driven Disasters, Berea A. Etherton, Robin A. Choudhury, Ricardo I. Alcalá Briseño, Romaric A. Mouafo-Tchinda, Aaron I. Plex Sulá, Manoj Choudhury, Ashish Adhikari, Si Lin Lei, Nattapol Kraisitudomsook, Jacobo Robledo Buritica

School of Earth, Environmental, & Marine Sciences Faculty Publications

Disaster plant pathology addresses how natural and human-driven disasters impact plant diseases, and the requirements for smart management solutions. Local to global drivers of plant disease change in response to disasters, often creating environments more conducive to plant disease. Most disasters have indirect effects on plant health through factors such as disrupted supply chains and damaged infrastructure. There is also the potential for direct effects from disasters, such as pathogen or vector dispersal due to floods, hurricanes, and human migration driven by war. Pulse stressors such as hurricanes and war require rapid responses, while press stressors such as climate change …

Skimming Genomes For Systematics And Dna Barcodes Of Corals, Andrea M. Quattrini, Luke J. Mccartin, Erin E. Easton, Jeremy Horowitz, Herman H. Wirshing, Hailey Bowers, Kenneth Mitchell, María Del P. González-García, Makiri Sei, Catherine S. Mcfadden

School of Earth, Environmental, & Marine Sciences Faculty Publications

Numerous genomic methods developed over the past two decades have enabled the discovery and extraction of orthologous loci to help resolve phylogenetic relationships across various taxa and scales. Genome skimming (or low-coverage genome sequencing) is a promising method to not only extract high-copy loci but also 100s to 1000s of phylogenetically informative nuclear loci (e.g., ultraconserved elements [UCEs] and exons) from contemporary and museum samples. The subphylum Anthozoa, including important ecosystem engineers (e.g., stony corals, black corals, anemones, and octocorals) in the marine environment, is in critical need of phylogenetic resolution and thus might benefit from a genome-skimming approach. We …

Green And Blue Water Footprints Of Major Crops In The Wami/Ruvu Basin, Tanzania: Implications For Water Scarcity And Sustainability, Benson Hm Lazaro, Martine M. Hagai, Rubhera Ram Mato

Tanzania Journal of Science

Accurate estimation of the blue and green water requirements of the various crops cultivated in a water basin is essential for planning specific plant/crop irrigation schedules. In this study, the crop water requirements of the major crops grown in the Wami/Ruvu basin were determined using the FAO-CROPWAT model based on meteorological parameters: monthly maximum and minimum temperature, wind speed, mean relative humidity, sunshine hours, rainfall data, and effective rainfall for 23 hydro-meteorological stations distributed in the study area. The studied crops are rice, maize, beans, tomatoes, and sugarcane. The crop reference evapotranspiration (ETo) and actual evapotranspiration (ETc) for each crop …

Cradle Explorer: Casfer Interactive Platform For Data And Model Visualization, Olatunde D. Akanbi, Vibha S. Mandayam, Haiping Ai, Arafath Nihar, Erika I. Barcelos, Laura S. Bruckman, Jeffrey Yarus, Yinghui Wu, Huichun (Judy) Zhang, Roger H. French

Student Scholarship

No abstract provided.

Arkansas Soil Erosion And Conservation Methods In Ornamental Landscapes, Abby J. Cutsinger

ATU Scholars Symposium

This study will attempt to identify the best soil erosion mitigation methods for Arkansas ornamental landscape settings by comparing the strengths and weaknesses of known techniques. Since soil is not a renewable resource, we must apply conservation practices wherever possible, not just in agricultural settings. To determine the most effective erosion prevention techniques, this study will review soil studies conducted by the NRCS and NASA, as well as articles on best practices observed in ornamental landscape and agricultural industries. These are analyzed by comparing and contrasting techniques against known problems with Arkansas soil to determine which methods are most effective. …

Development Of Novel Analytical Methods For The Investigation Of Mammalian Tissues And Cells, Grace A. Binder

USF Tampa Graduate Theses and Dissertations

This dissertation endeavors to advance the frontiers of analytical chemistry by addressing critical challenges across three distinct chapters. In the first chapter, the focus is on the evaluation of digestion methods for the analysis of trace metals in mammalian tissues and the Standard Reference Material NIST 1577c. The primary goal is to optimize methodologies, laying the groundwork for reliable trace metal analysis and furthering our understanding of their roles in biological systems.

Transitioning into the realm of immunomodulation, the second chapter elucidates immune response, disease and treatment through the optimization and discovery of STING modulators, utilizing the luciferase assay for …

Influence Of Low-Level Condensed Tannins Concentrations In Temperate Forages On Sheep Performace, F Montossi, F Liu, J Hodgson, S T. Morris, T N. Barry, D F. Risso

IGC Proceedings (1977-2023)

Results from a series of six comparative lamb grazing trials with temperate forages are summarised, and compared with results from the literature. Condensed tannin (CT) concentrations in the diet ranged from 0.12% to 0.47% on a dry matter (DM) basis. Values were generally similar for Yorkshire fog (Holcus lanatus), perennial ryegrass (Lolium perenne) and annual ryegrass (L. multiflorum), each grown with white clover (Trifolium repens), in similar circumstances. Concentrations for tall fescue (Festuca arundinacea)/white clover pastures were relatively low, but may have been artefacts. Drenching with polyethylene glycol (PEG) to bind …

Tannin Contents Of Some Indigenous Browse Plants Of Botswana, A A. Aganga, C M. Tsopito, K M. Morake

IGC Proceedings (1977-2023)

A study was carried out to evaluate the tannin content and crude protein degradation of leaves and twigs from indigenous browsable trees which provide feed for grazing livestock on natural rangelands of Botswana. These browses include the families Leguminosae (Acacias), Capparidaceae (Boscias), Combretaceae (Combretum) and Tiliaceae (Grewias). These were found to be of good nutritive value, with the leaves having a high content of crude protein (9 - 16%) and a wide range of dry matter digestibility (37 - 69.82%). The correlation between crude protein disappearance and tannin content was found to be negative (-0.582) and significant (P<0.05).

Effect Of Tannin In Animal Diet On Nitrification Rate Of Pasture Soil Under Dung Patches, J R. Crush

IGC Proceedings (1977-2023)

The rate of formation of nitrate and ammonium in the soil under dung patches was compared for dung from animals fed either ryegrass (Lolium perenne)/white clover (Trifolium repens) or ryegrass/Lotus pedunculatus forage. Tannin derived from the Lotus did not inhibit ammonification in the soil under dung but nitrate formation was strongly inhibited. The results support the theory that polyphenolics may bind to nitrite in acid soils and cause immobilisation of mineral nitrogen. Apart from their effect of reducing the proportion of dietary nitrogen excreted in urine, tannins in animal diets may make a further contribution to …

Condensed Tannins In Tropical Legumes: Concentration, Astringency And Effects On The Nutrition Of Ruminants, R Barahona, C E. Lascano, R C. Cochran, J L. Morrill, E C. Titgemeyer

IGC Proceedings (1977-2023)

A feeding trial was carried out to determine the effect of extractable condensed tannins (ECT) concentration and tannin astringency in tropical legumes on nitrogen (N) digestion by sheep. Test legumes were Desmodium ovalifolium (Do) and Flemingia macrophylla (Fm) which had similar concentrations of Extractable CT (9% DM) but tannins with different degree of astringency (Do, 0.6 and Fm, 0.3 g protein bound/g of ECT). Chopped sun-dried forage of each legume was sprayed with either water (control) or polyethylene glycol (PEG, 3.5% DM) to reduce ECT and fed to 8 sheep with ruminal and duodenal canulas arranged in a replicated 4 …

Effect Of Condensed Tannin In Lotus Corniculatus And Lotus Pedunculatus On Digestion Of Rubisco In The Rumen, W C. Mcnabb, R J. Aerts, A Brand, J S. Peters, Y L. Foo, G C. Waghorn

IGC Proceedings (1977-2023)

The in vitro precipitation of ribulose-1,5-bisphosphate carboxylase (Rubisco) by condensed tannin (CT) extracted from Lotus corniculatus and Lotus pedunculatus and the effect of these CT on the in vitro rumen degradation of Rubisco was used to compare the reactivity of these CT. The chemical structure of CT from Lotus corniculatus was homogenous with epicatechin stereochemistry and mostly procyanidin units. The CT from Lotus pedunculatus was heterogenous with mostly prodelphinidin units. The amount of CT required to precipitate all the Rubisco when total soluble leaf protein was incubated with CT from Lotus corniculatus and Lotus pedunculatus was similar. Although CT from …

Nitrogen And Fiber Digestion In Sheep Fed Fresh-Frozen And Field-Dried High And Low Tannin Sericea Lespedeza, T H. Terrill, W R. Windham, C S. Hoveland, H E. Amos, B Kouakou, S Gelaye

IGC Proceedings (1977-2023)

Field-drying reduces extractable condensed tannin (CT) of sericea lespedeza [Lespedeza cuneata (Dum-Cours) G. Don], but effects on specific bound and unbound forms of CT and the relationship to protein and fiber digestion in ruminants are unclear. Intake and digestibility of fresh-frozen and field-dried high- and low-tannin sericea were investigated in a feeding trial with sheep (Ovis spp.). Field-drying had no effect on crude protein (CP) and fiber content of sericea, but shifted CT from extractable to bound forms. Digestion coefficients for CP, neutral detergent fiber (NDF), and acid detergent fiber (ADF) were lower for high-tannin sericea than the low-tannin …

Alteration Of Condensed Tannin Sythesis In Transgenic Forage Legumes, F Paolocci, G Tanner, S Arcioni, F Damiani

IGC Proceedings (1977-2023)

The transformation of Lotus corniculatus plants with the maize gene Sn, reorganizes the tissue specificity of condensed tannins accumulation. In particular the transformed plants show an increase of tannin content in roots and a decrease in leaves. Molecular and enzymatic analyses suggest that the transgene can functionally substitute an endogenous unknown gene not expressed in roots and induces its silencing when it is expressed. These findings could have applications for reducing tannin content in unpalatable plants and for cloning genes involved in tannin synthesis.

Comparison Of The In Vitro Fermentation Characteristic Of Fractionated Alfalfa And Sainfoin, L R. Mcmahon, M Goto, T A. Mcallister, G A. Jones, K J. Cheng

IGC Proceedings (1977-2023)

Alfalfa and sainfoin leaves were lyophilized and ground (A, S, respectively) or were fractionated into soluble (A_SOL, S_SOL) and insoluble (A_INS, S_INS)components and lyophilized and incubated in vitro with diluted ruminal fluid for 24h. Whole leaf and soluble fractions were also incubated with polyethylene glycol 8000 (PEG). Gas production (GP), ammonia concentration [NH3] and volatile fatty acid (VFA) production were lower (P< 0.05) from S and SSOL than from A and ASOL Insoluble fractions differed only in [NH3] (SINS < AINS, P < 0.05). Inclusion of PEG increased (P < 0.05) GP, [NH3] and VFA production from whole leaf and soluble fractions of sainfoin, but not alfalfa. Inactivating the condensed tannins in sainfoin with PEG overcame differences in degradability between these two forages.

Differences In Chemical Composition Among Provenances Of Tree Fodder Species In A Subhumid Environment: Relation To Use As Supplements, B H. Dzowela, L Hove, P L. Mafongoya

IGC Proceedings (1977-2023)

Studies were initiated in a subhumid Southern African environment to assess the chemical composition and nutritive value of Acacia angustissima (Mill.) Kuntze, Calliandra calothyrsus Meiss. and Leucaena species. The objective was to determine variability in crude protein (CP) concentration, acid detergent fibre (ADF), neutral detergent fibre (NDF), and soluble and insoluble proanthocyanidins from fodder samples of species, subspecies and provenances. A wide range was found in these parameters. The implications of these chemical composition factors, especially proanthocyanidins, on the use of these browse fodders in livestock feeding systems are discussed.

Nutritional Implications Of Bound Proanthocyanidins, H P.S Makkar, K Becker

IGC Proceedings (1977-2023)

The correlation between protein binding capacity of proanthocyanidin-rich neutral detergent fiber (NDF) and their proanthocyanidin (PAs) content was very low (r = -0.21; n = 6). These PAs-rich NDF did not affect growth of a tannin-sensitive strain of Clostridium perfringens. A substantial amount of bound PAs (75 to 92 %) disappeared from the NDF in 24 h of fermentation in an in vitro incubation medium containing rumen microbes. Addition of a tannin-binding agent, polyethylene glycol to the incubation medium containing rumen microbes and browses made free of extractable tannins or NDF rich in bound PAs increased the gas production up …

Environmental Effect On New Released Genotypes Of Graminaceous And Leguminous Forage Perennial Crops, P Martiniello, G Genier, R Allerit

IGC Proceedings (1977-2023)

Genotypes of perennial leguminous (alfalfa) and graminaceous (cocksfoot and tall fescue) were evaluated for adaptability in a typical Mediterranean environment (Foggia, southern Italy). The species were field evaluated to detect biomass yield potential under different types of watering. Irrigations were applied considering rainfall and FAO cultural growth coefficient for reintegrating the 75% of the evaporated water from Class A water pan. The forage harvest was applied when the shoots of the varieties reached the 10 and 50% of flowering and heading stage for leguminous and graminaceous, respectively. A split-plot experimental design, with irrigation in the main plot, was adopted. The …

Plant Tannins- Their Role In Forage Legume Quality, J A. Alokan, V A. Aletor

IGC Proceedings (1977-2023)

Forage legumes, especially browse plants, are important in the maintenance and survival of ruminant animals. However many browse species contain high levels of polyphenolic compounds including tannins. The effects of tannins on the quality of forages are reviewed. These include reduction in palatability and intake of feeds, growth rate, digestion of fibre fractions and nitrogen utilization by animals.

Genetic Manipulation Of Sainfoin Leaf Tannins, M Y. Gruber, P Auser, G Lees, A D. Muir, M Wood

IGC Proceedings (1977-2023)

An isogenic population of sainfoin (Onobrychis viciifolia Scop.) plants was developed by two methods; regeneration after long term tissue culture and plant transformation with an antisense chalcone synthase gene. Tannin (proanthocyanidin) content could be dramatically increased in untransformed callus by long term culture, but was not significantly altered in regenerating plantlets. Some of the mature transgenic plants had dramatically reduced levels of leaf tannin. The antisense gene was eliminated from all transgenic plants later in development.

Induction Of Mutants With Ectopic Expression Of Condensed Tannins, M Y. Gruber, B Skadhauge, P Auser, A D. Muir, K K. Thomsen, J Stougaard, B Coulman, D Von Wettstein

IGC Proceedings (1977-2023)

Leaves of 47,000 Lotus japonicus plants were screened using a butanol:HCl histochemical test to select “gain of function” mutants. These plants were progeny from L. japonicus lines which were transformed with T-DNA constructs containing either the maize Ds or Ac transposon (Thykjaer et al., 1995). Among 21 putative leaf tannin mutants, five (tan1-5) were characterized for synthesis of condensed tannins, leucocyanidin reductase activity and the presence of Ac and the selectable marker gene, nptII . A range of leaf tannin content among other Lotus species was also characterized.

A Revised Method Of Condensed Tannin Analysis In Leucaena Spp., S A. Dalzell, G L. Kerven

IGC Proceedings (1977-2023)

The proanthocyanidin (butanol-HCl) assay was used to measure condensed tannin (CT) in extracts from Leucaena spp. at the University of Queensland. Inconsistent results were found to be caused by the iron catalyst in the butanol/HCl reagent and the presence of ascorbic acid in the sample matrix which enhanced anthocyanidin development. In addition, preparation of sample extracts by back extraction with organic solvents reduced CT recoveries. An accurate and rapid technique was developed that measures CT directly in 70% aqueous acetone 0.1% (w/v) sodium metabisulphite plant extracts.

The Effect Of Condensed Tannins In Lotus Spp. On The Lesser Migratory Grasshopper, C F. Hinks, G L. Lees, M Y. Gruber, A D. Muir, J Soroka

IGC Proceedings (1977-2023)

Hatchling grasshoppers (Melanoplus sanguinipes Fab.) fed one accession of field grown big trefoil (Lotus uliginosis Schkuhr) containing a tannin content of 56 mg. g-1 FW, had a significantly lower mean weight than hatchlings fed field grown Lotus species and accessions with lower tannin content. Adult female grasshoppers fed L. uliginosis foliage with 32.3 mg.g-1 FW tannin also ate more feed and had a 2-fold higher gut trypsin and chymotrypsin activity than adults fed low tannin L. corniculatus foliage (0.13 mg.g-1 FW tannin). In more controlled experiments, growth at 20o C or 30o C was used to increase tannin …

No "Butts" About It: The Heavy Metal Impact Of Cigarette Litter, Faith Huff, Connor Monson, Spencer Whittington

23rd Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2024)

Cigarettes are the most common litter globally. Following smoking, the filter is left with carcinogenic compounds, tar and metals. All of which are highly toxic for wildlife, affecting reproduction, behavior and mortality rate in animals. The heavy metals that are left within the filter also have potential to leach from the cigarettes and enter the groundwater system. As these heavy metals enter the system, it leads to adverse effects on the environment and can cause detrimental health effects in humans, such as cell damage, induced carcinogenic processes and loss of cellular functions. Much research that exists currently only focuses on …

Revolutionizing Ecosystems: Innovative Planting Machines Combating Desertification, Sunghun Park, Jeanne Sumrall

SACAD: Scholarly Activities

An important environmental issue that has a profound effect on human welfare, socioeconomic stability, and global ecosystems is desertification. A little over 12 million hectares of land perish year due to the damaging impacts of desertification, which is made worse by overgrazing, deforestation, and inadequate water management. This not only results in a significant loss of arable land, capable of producing 20 million tons of grain, but also in substantial economic repercussions, with estimated annual losses reaching USD 42 billion (UNCCD, 2020). The enormity of the problem emphasizes how urgently we need practical, long-term solutions to stop and reverse the …

Developmental Characteristics And Renovating Pattern Of Reed Canarygrass Shoots In A Sward, M Ito, S Mizuno, T Otani, S Kobayashi

IGC Proceedings (1977-2023)

Developmental and renovating traits of shoots were investigated ecomorphologically in a reed canarygrass (Phalaris arundinacea L., cv. Palaton) sward harvested 3 times per year. Reed canarygrass shoots exhibited notably high growth rates both in the 1st and 2nd crops together with rapid leaf formation (around 7 days/leaf) and active internode elongation. Vigorously growing shoots, which regenerated soon after the 1st cutting and dominated in the 2nd crop canopy, mainly originated from the stubble formed with 3 to 4 phytomers that developed after the beginning of spring growth, thus resulting in higher shoot density than that of the 1st crop. …

Canopy Changes In Healthlands (Erica-Ulex) Grazed By Sheep Or Goats, R Celaya, K Osoro

IGC Proceedings (1977-2023)

The grazing effects of sheep and goats on vegetation dominated by heaths (Erica spp) and gorse (Ulex gallii) were studied for two years. Two plots were grazed from May to October 1992 by 7 ewes or 7 does and in the following year each plot was subdivided into two subplots subsequently grazed either by sheep or by goats in a factorial design. During the first grazing season both sheep and goats reduced similarly the cover percentage of shrubs since the herbaceous content was scarce, although goats reduced more the height of the shrubs than sheep. The cover …

Effect Of Nitrogen, Phosphorus, Potassium And Cutting On The Regrowth And Insect Infestation Of A Sorghum And Berseem Clover In Egypt, M B. Attia

IGC Proceedings (1977-2023)

The objective of this study was to determine the minimum quantities of phosphorus and potassium which must be applied with varying amounts of nitrogen in order to maintain maximum hay production and minimum level of insect infestation. This was more pronounced at the higher N rates and caused a significant N x K interaction during each of these years. There was no yield response to applied phosphorus at any N rate. Relation between levels of nitrogen and infestation percentage was positive but infestation rate decreased with the increase of potassium levels while there was no relation between levels of phosphorus …

Cynodon Dactylon Control In Conservation Tillage Systems, A Rios, J M. Sanz

IGC Proceedings (1977-2023)

Bermudagrass (Cynodon dactylon L. PERS.) is the most important weed in Uruguay. With the development of conservation tillage systems, chemical control of bermudagrass becomes essential. The objective of this research was to evaluate, in a long term integrated program, bermudagrass chemical control during the first year of a pasture. Application of glyphosate and no-tillage seeding of the pasture took place during fall. A 70% reduction of underground part of the weed was detected. The chemical control, together with a conservation tillage system and an adequate pasture management allowed an important reduction in bermudagrass level for the first year pasture. …

Legume Seedling Development In No Till Pasture Establishment, J L. Rossi, V A. Deregibus

IGC Proceedings (1977-2023)

The objective of this study was to compare no-till and conventional seeding of pasture and their effects on legume establishment. The presence of residue cover reduced legume seedling establishment and weed population under no-till seeding. The reduction in establishment was associated with the physical impediment of the residue cover and the decay of seedling vigor when passing through that cover.

Ecotypic Adaptation Of Medicago Polymorpha Along A Gradient In Central Chile: Growth And Seed Production, A Del Pozo, C Ovalle, J Avendano

IGC Proceedings (1977-2023)

Relative growth rate, total dry weight and seed yield on 19 accessions of burr medic (Medicago polymorpha L.) from different bioclimatic conditions, were assessed at Cauquenes (35°58’;72°17’W) in the subhumid mediterranean region. The accessions were sown in microplots over raised beds during two years (1991 and 1992), and swards cut periodically at 2, 6 and 10 cm height. There were significant differences between accessions in both total dry weight and seed yield (P < 0.001, Table 1), but the interaction between accessions and height of cutting was not significant (P > 0.05). Total dry weight was positive correlated with days to flowering (r2 = 0.81; P > 0.01).