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Other Orienteering Problem Variants, Pieter Vansteenwegen, Aldy Gunawan Aug 2025

Other Orienteering Problem Variants, Pieter Vansteenwegen, Aldy Gunawan

Research Collection School Of Computing and Information Systems

In this chapter, different variants of routing problems with profits will be discussed. Based on what is available in literature, mostly variants of the orienteering problem will be discussed. A first variant considers capacity constraints, since these appear frequently in many practical applications. Next, multi-objective orienteering problems, explicitly considering different types of profits separately are discussed. Time-dependent and stochastic travel times are also relevant for most practical applications. These are considered together with time-dependent and stochastic profits. More and more routing problems are considered together with inventory management. For routing problems with profits, this leads to the inventory orienteering problem, …


Causalabstain: Enhancing Multilingual Llms With Causal Reasoning For Trustworthy Abstention, Yuxi Sun, Aoqi Zuo, Wei Gao, Jing Ma Aug 2025

Causalabstain: Enhancing Multilingual Llms With Causal Reasoning For Trustworthy Abstention, Yuxi Sun, Aoqi Zuo, Wei Gao, Jing Ma

Research Collection School Of Computing and Information Systems

Large Language Models (LLMs) often exhibit knowledge disparities across languages. Encouraging LLMs to abstain when faced with knowledge gaps is a promising strategy to reduce hallucinations in multilingual settings. Current abstention strategies for multilingual scenarios primarily rely on generating feedback in various languages using LLMs and performing self-reflection. However, these methods can be adversely impacted by inaccuracies and biases in the generated feedback. To address this, from a causal perspective, we introduce CausalAbstain, a method that helps LLMs determine whether to utilize multiple generated feedback responses and how to identify the most useful ones. Extensive experiments demonstrate that CausalAbstain effectively …


Advancing Molecular Graph-Text Pre-Training Via Fine-Grained Alignment, Yibo Li, Yuan Fang, Mengmei Zhang, Chuan Shi Aug 2025

Advancing Molecular Graph-Text Pre-Training Via Fine-Grained Alignment, Yibo Li, Yuan Fang, Mengmei Zhang, Chuan Shi

Research Collection School Of Computing and Information Systems

Understanding molecular structure and related knowledge is crucialfor scientific research. Recent studies integrate molecular graphswith their textual descriptions to enhance molecular representationlearning. However, they focus on the whole molecular graph andneglect frequently occurring subgraphs, known as motifs, whichare essential for determining molecular properties. Without suchfine-grained knowledge, these models struggle to generalize to un-seen molecules and tasks that require motif-level insights. To bridgethis gap, we propose FineMolTex, a novel Fine-grained Moleculargraph-Text pre-training framework to jointly learn coarse-grainedmolecule-level knowledge and fine-grained motif-level knowledge.Specifically, FineMolTex consists of two pre-training tasks: a con-trastive alignment task for coarse-grained matching and a maskedmulti-modal modeling task for …


Equivalence And Similarity Refutation For Probabilistic Programs, Krishnendu Chatterjee, Ehsan Kafshdar Goharshady, Petr Novotný, Dorde Zikelic Aug 2025

Equivalence And Similarity Refutation For Probabilistic Programs, Krishnendu Chatterjee, Ehsan Kafshdar Goharshady, Petr Novotný, Dorde Zikelic

Research Collection School Of Computing and Information Systems

We consider the problems of statically refuting equivalence and similarity of output distributions defined by a pair of probabilistic programs. Equivalence and similarity are two fundamental relational properties of probabilistic programs that are essential for their correctness both in implementation and in compilation. In this work, we present a new method for static equivalence and similarity refutation. Our method refutes equivalence and similarity by computing a function over program outputs whose expected value with respect to the output distributions of two programs is different. The function is computed simultaneously with an upper expectation supermartingale and a lower expectation submartingale for …


Graph Positional Autoencoders As Self-Supervised Learners, Yang Liu, Deyu Bo, Wenxuan Cao, Yuan Fang, Yawen Li, Chuan Shi Aug 2025

Graph Positional Autoencoders As Self-Supervised Learners, Yang Liu, Deyu Bo, Wenxuan Cao, Yuan Fang, Yawen Li, Chuan Shi

Research Collection School Of Computing and Information Systems

Graph self-supervised learning seeks to learn effective graph representations without relying on labeled data. Among various approaches, graph autoencoders (GAEs) have gained significant attention for their efficiency and scalability. Typically, GAEs take incomplete graphs as input and predict missing elements, such as masked node features or edges. Although effective, our experimental investigation reveals that traditional feature or edge masking paradigms primarily capture low-frequency signals in the graph and fail to learn expressive structural information. To address these issues, we propose Graph Positional Autoencoders (GraphPAE), which employ a dual-path architecture to reconstruct both node features and positions. Specifically, the feature path …


Ai-Assisted Risk Assessment In Generative Ai Governance, Wu Jiaqi Young, Fiona Fui-Hoon Nah Aug 2025

Ai-Assisted Risk Assessment In Generative Ai Governance, Wu Jiaqi Young, Fiona Fui-Hoon Nah

Research Collection School Of Computing and Information Systems

Effective risk assessment is paramount for responsible generative AI (GenAI) deployment. Traditional governance approaches that rely on manual reviews are inadequate given the scale and velocity of GenAI outputs. A risk-based approach incorporating real-time monitoring and governance is paramount. In this research, we examine how the efficacy of suggestive versus supportive explanations for AI’s risk assessment of GenAI outputs is moderated by user domain expertise and AI’s risk assessment in determining user acceptance. We hypothesize that cognitive involvement increases with AI’s risk assessment, with higher risks triggering more critical evaluation. By drawing on the elaboration likelihood model, we hypothesize that …


Wildfires Classification In Canadian Boreal Forest: A Comparative Study Of Logistic Regression And Xgboost Models, Brandon Tran, Elijah James Duran, Mike Luu, Hesham Morgan, Surendra Maharjan, Wenzhao Li, Hesham El-Askary Aug 2025

Wildfires Classification In Canadian Boreal Forest: A Comparative Study Of Logistic Regression And Xgboost Models, Brandon Tran, Elijah James Duran, Mike Luu, Hesham Morgan, Surendra Maharjan, Wenzhao Li, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

In recent years, Canada has faced a growing number of wildfires. These events have devastated ecosystems, displaced communities, and posed severe health risks. To minimize the damage caused by such disasters, this study aims to develop an early warning system that predicts wildfire occurrences. Two machine learning models for binary classification of wildfire occurrence in Canadian wild forests, Logistic regression and XGBoost, will be compared and evaluated. The models are used to predict the likelihood of wildfire events based on various environmental and climatic factors. The models are evaluated using a 70-30 split validation approach and their performance is assessed …


Unfolding Particle Detector Effects And Solving Qcd Inverse Problem With Generative Ai, Tareq Saeed Alghamdi Aug 2025

Unfolding Particle Detector Effects And Solving Qcd Inverse Problem With Generative Ai, Tareq Saeed Alghamdi

Computer Science Theses & Dissertations

Advancements in artificial intelligence (AI) have revolutionized high-energy physics by enabling generative models to address key detector-related Challenges. This work explores the generative model to mitigate smearing, acceptance, and inefficiency in particle detectors, enhancing experimental precision.

We present a generative model-based framework to model and correct detector distortions. Using the Jefferson Lab CLAS g11 experiment as a case study, our approach successfully unfolds detector effects in multi-particle final states while preserving multidimensional correlations despite complex reaction mechanisms. A key focus is addressing the acceptance problem—accurately modeling detector acceptance without computationally expensive simulations. By training generative model-based framework on simulated detector …


Roadside Asset Extraction From Mobile Lidar Point Cloud, Yushin Ahn, Riadh Munjy, Stephen Choi Aug 2025

Roadside Asset Extraction From Mobile Lidar Point Cloud, Yushin Ahn, Riadh Munjy, Stephen Choi

Mineta Transportation Institute

Mobile LiDAR systems are powerful tools that help us map roads and their surroundings in 3D with great speed and precision. The data provided by these systems support urban planning efforts, digital mapping, transportation infrastructure maintenance, and more. This report presents a comprehensive workflow for roadside asset extraction using Mobile Terrestrial Laser Scanning (MTLS) data, focusing on road lane detection, cross-section slope analysis, and point cloud classification. Roadside asset extraction is the identification and classification of roadside features like signs and poles. The dataset, acquired using a high-resolution mobile LiDAR system, contains over 5.7 billion points (pieces of data) across …


Complex System Governance And Cyber Operations, Willie Gernard Mccallister Aug 2025

Complex System Governance And Cyber Operations, Willie Gernard Mccallister

Engineering Management & Systems Engineering Theses & Dissertations

This dissertation examines the potential integration of Complex System Governance (CSG) within cybersecurity, emphasizing the development of a reference model for Cybersecurity Infrastructures. Traditional strategies for securing digital environments have struggled to address the intricate and dynamic layers inherent in modern cybersecurity systems. The purpose of this research is to explore the applicability of CSG as a framework to assess cybersecurity infrastructure using a case study research design. The research addresses two key questions: (1) How can the CSG reference model be adapted to explore cybersecurity infrastructure? (2) What results from CSG based exploration of cybersecurity infrastructure through a case …


Enhancing Non-Visual Interaction With Online User-Generated Content, Mohan Krishna Sunkara Aug 2025

Enhancing Non-Visual Interaction With Online User-Generated Content, Mohan Krishna Sunkara

Computer Science Theses & Dissertations

The Web has become the dominant medium for our everyday activities, including communication, business, e-commerce, news, and entertainment. Consequently, the online world is experiencing an explosion of User-Generated Content (UGC), particularly on social media platforms and online review systems. To facilitate convenient interaction with UGC, web platforms have adopted various presentation strategies that enable users to efficiently browse and contribute to the UGC. However, these user interfaces are primarily designed for sighted individuals, so they do little to assist blind users who rely predominantly on audio-based screen reader assistive technology. The extant efforts to improve web interaction for blind users …


Human Activity Recognition And Identification Driven Automated Deep Learning For Time-Series Classification, Justin Alan Gamble Aug 2025

Human Activity Recognition And Identification Driven Automated Deep Learning For Time-Series Classification, Justin Alan Gamble

Engineering Management & Systems Engineering Theses & Dissertations

The growing emphasis on Digital Engineering (DE) within the U.S. Department of Defense (DoD) demands advanced methods for leveraging vast time-series data generated by sensor-rich environments. Deep learning models offer promising solutions for complex timeseries classification tasks, however their design and optimization remain highly resource intensive, requiring specialized expertise. This dissertation addresses this challenge by developing and evaluating an Automated Machine Learning (AutoML) framework specifically tailored for the time-series classification task of Human Activity Recognition and Identification (HARI).

A systematic investigation was conducted using the Design Science Research Methodology (DSRM) comparing traditional search strategies of grid search and random search …


Input Structure Based Optimization For Privacy Preserving Ai Systems, Feng Yizhou Aug 2025

Input Structure Based Optimization For Privacy Preserving Ai Systems, Feng Yizhou

Electrical & Computer Engineering Theses & Dissertations

As Artificial Intelligence (AI) systems become increasingly integrated into critical domains, ensuring privacy-preserving model design and system deployment has become a pressing priority. Safeguarding both sensitive user data and proprietary model parameters is critical throughout the AI model and system, from data acquisition and pre-processing to model inference and deployment. However, existing privacy-preserving frameworks face several limitations, including fragmented data ownership, incomplete protection across system stages, substantial computational overhead, and poor scalability to modern architectures such as large language models. This dissertation explores a unifying optimization strategy centered on input structure design to address these challenges. The core idea is …


Short: Breaking The Charge: Exploiting State Manipulation In Ev Charging, Ce Zhou, Qiben Yan, Zhiyuan Yu, Eshan Dixit, Ning Zhang, Huacheng Zeng, Alireza Safdari Ghanhdari Aug 2025

Short: Breaking The Charge: Exploiting State Manipulation In Ev Charging, Ce Zhou, Qiben Yan, Zhiyuan Yu, Eshan Dixit, Ning Zhang, Huacheng Zeng, Alireza Safdari Ghanhdari

Computer Science Faculty Research & Creative Works

Electric vehicles (EVs) have become one of the promising solutions to the ever-evolving environmental and energy crisis. The key to the wide adoption of EVs is a pervasive charging infrastructure, composed of both private/home chargers and public/commercial charging stations. However, the security of electric vehicle charging has not been thoroughly investigated. This paper investigates the communication mechanisms between the chargers and EVs and exposes the lack of protection for the authenticity in the SAE J1772 charging control protocol. To showcase our discoveries, we propose a new class of attacks, ChargeX, which aims to manipulate the charging states of EV chargers …


From Supercomputers To Desktops: An Interactive And Portable System For Particle In Cell Simulation And Visualization Using Commodity Hardware, Kim Peterson Aug 2025

From Supercomputers To Desktops: An Interactive And Portable System For Particle In Cell Simulation And Visualization Using Commodity Hardware, Kim Peterson

All Graduate Theses and Dissertations, Fall 2023 to Present

Generating and analyzing visual representations of simulation data in real time (i.e., during execution), has become increasingly important for handling the complexity and scale of modern computational models. Traditionally, this approach has been limited to High-Performance Computing (HPC) environments, leaving some researchers, students and educators without resources to explore cutting-edge simulations and analyses. This work attempts to show that powerful scientific simulations and real-time analysis can be made available by using more easily obtained commodity hardware. This approach may enable a broader participation in the scientific computing discovery process.

In this paper, we explore how advanced simulation and visualization frameworks …


A Focus On Student Education: Determining Student Attitudes Towards Transparent Autograding And Developing Artificially Intelligent Tools To Help Students Succeed, Andra Rice Aug 2025

A Focus On Student Education: Determining Student Attitudes Towards Transparent Autograding And Developing Artificially Intelligent Tools To Help Students Succeed, Andra Rice

All Graduate Theses and Dissertations, Fall 2023 to Present

This thesis is composed of two parts both relating to helping students succeed. First, the focus is on determining how we can help students feel more comfortable using an AI tool that can provide them immediate feedback. Second, machine learning algorithms are explored in relation to tracking student tasks to encourage healthy study habits.

The development of effective autograders is key for scaling assessment and feedback. While AI based autograding systems for open-ended response questions have been found to be beneficial for providing immediate feedback, autograders are not always liked, understood, or trusted by students. Our research tested the effect …


Out Of Core And Adaptive Image Blending Approach For Large Scale Image Mosaics, Marcus Quincy Aug 2025

Out Of Core And Adaptive Image Blending Approach For Large Scale Image Mosaics, Marcus Quincy

All Graduate Theses and Dissertations, Fall 2023 to Present

When creating large stitched images, like those used in maps made from aerial photos, it’s important to make sure the seams between individual pictures aren’t visible. This process, known as color blending, helps smooth out differences in lighting or weather across the images. But blending very large images, such as those made from many high-resolution aerial photos, can require huge amounts of memory, making it hard to do on a typical computer.

In this work, we developed a method that breaks the problem into smaller pieces, so only a small part of the image needs to be worked on at …


Evaluating The Effects Of 3d User Interactions And Virtual Displays On Human Cognition And Perception For Immersive Analytics, Dongyun Han Aug 2025

Evaluating The Effects Of 3d User Interactions And Virtual Displays On Human Cognition And Perception For Immersive Analytics, Dongyun Han

All Graduate Theses and Dissertations, Fall 2023 to Present

This research investigates how virtual reality (VR) can be utilized effectively for users to explore data. Traditional data analysis typically takes place on flat, two-dimensional computer screens. In contrast, immersive technologies like VR offer a three-dimensional environment where users can engage with data more naturally and intuitively. Such immersive experiences have the potential to improve comprehension, memory, and insight during data analysis tasks. Despite this potential, several challenges remain in making immersive analytics practical and effective. Key questions include which types of 3D interaction techniques are most helpful and how accurately people perceive visual information in immersive environments. To address …


Trust In Healthcare Ai Can’T Just Be Designed – It Must Be Felt By Clinicians And Patients, Adriana Banozic-Tang, Heng Wang Aug 2025

Trust In Healthcare Ai Can’T Just Be Designed – It Must Be Felt By Clinicians And Patients, Adriana Banozic-Tang, Heng Wang

Research Collection Yong Pung How School Of Law

Trust in healthcare AI currently over-relies on system design, not lived medical realities.Continuous feedback loops are necessary to embed trust in healthcare AI that is responsive to clinician and patient needs.Initiatives in South-East Asia show how trust in technology can be extended from policy to practice.


Ai In The Judiciary: The Singapore Case, Nydia Remolina Leon Aug 2025

Ai In The Judiciary: The Singapore Case, Nydia Remolina Leon

Research Collection Yong Pung How School Of Law

This paper examines the integration of Artificial Intelligence (AI) within the judicial system of Singapore. Singapore's judiciary has embraced AI not as a tool for adjudication, but as an augmentative instrument for legal research, procedural efficiency, and access to justice. It provides a detailed account of AI use cases in the courts, including case summarization, evidence review, assistance for selfrepresented litigants, and tools like the Divorce Assets Informative Division Estimator. The discussion then turns to the legal profession, exploring how law firms in Singapore are adopting AI technologies. The paper also addresses how AI implementation in the judicial system is …


Faced With Genai, Educators’ Engagement Capacity Matters More Than Ever, Thomas Menkhoff Aug 2025

Faced With Genai, Educators’ Engagement Capacity Matters More Than Ever, Thomas Menkhoff

Research Collection Lee Kong Chian School Of Business

In a commentary, SMU Professor of Organisational Behaviour & Human Resources (Education) Thomas Menkhoff stressed the need for educators to upskill so they can guide students in using generative artificial intelligence (GenAI) responsibly, rather than dismissing it. He argued that universities should move beyond prohibition and invest in AI literacy to safeguard academic integrity. Prof Menkhoff mentioned that combining the use of GenAI tools with effective prompting and Socratic questioning transforms students’ use of technology from passive consumption to active, reflective and critical engagement. To achieve this, he said that schools must set clear guidelines and design AI-compatible assessments that …


A Value Sensitive Design Approach To Reimagining Parental Control Apps, Prakriti Dumaru Aug 2025

A Value Sensitive Design Approach To Reimagining Parental Control Apps, Prakriti Dumaru

All Graduate Theses and Dissertations, Fall 2023 to Present

Parental control apps are often used by families to regulate children's device usage and keep them safe online. However, existing tools focus on monitoring and restricting children, which can lead to tension and mistrust within families. This research takes a new approach by exploring how parental control apps can be redesigned to support positive family values, like encouraging open communication between parents and children and helping children self-regulate their behaviors. Through four in-depth studies, this dissertation looks at how these values play out in different real-life situations. It includes families with children on the autism spectrum, divorced households where parents …


Beyond The Click: Investigating Mental Models, Targeted Attacks, And Behavioral Interventions Against Clickbait, Ankit Shrestha Aug 2025

Beyond The Click: Investigating Mental Models, Targeted Attacks, And Behavioral Interventions Against Clickbait, Ankit Shrestha

All Graduate Theses and Dissertations, Fall 2023 to Present

Clickbait is misleading or exaggerated content on social media that tricks people into clicking on links by making them curious. For instance, posts that use headlines such as “This is the worst day to visit a restaurant”. These clickbait posts can lead to fake news, wrong information, and even harmful websites. Even though many people know clickbait can be risky, they often still fall for it. Existing tools to stop clickbait don’t always consider how people think, the situations they are in, or their different needs. This research looks at how people understand and react to clickbait, and develops different …


Low-Level Memory Attacks On Edge Assisted Robotic Applications, William Arnold Aug 2025

Low-Level Memory Attacks On Edge Assisted Robotic Applications, William Arnold

Master of Engineering Theses

This thesis investigates how low-level memory faults can undermine edge-assisted robotic systems that rely on memory optimization. As robots are utilized in real world applications, the ability to operate safely and successfully in mission critical deployment becomes important. To help achieve these goals, developers are increasingly starting to place computation nodes at network edges to meet latency and reliability requirements. Edge nodes, however, are resource-constrained and resources conservation techniques such as Kernel Same-page Merging (KSM) are enabled to deduplicate identical pages across processes or virtual machines. This thesis shows that this optimization technique quietly widens the attack surface and can …


Advancing Real-World Implementation Of The Well Optimized Linear Finder (Wolf) High-Speed Atmospheric Turbulence Compensation Method, Timothy Evan Coon Aug 2025

Advancing Real-World Implementation Of The Well Optimized Linear Finder (Wolf) High-Speed Atmospheric Turbulence Compensation Method, Timothy Evan Coon

Theses and Dissertations

This dissertation advances the real-world implementation of the Well Optimized Linear Finder (WOLF) method for high-speed Atmospheric Turbulence Compensation (ATC). Atmospheric turbulence introduces phase aberrations into optical wavefronts and degrades image quality in terrestrial imaging systems. Traditional phase diversity methods are computationally intensive and poorly suited to real-time operation. The WOLF method addresses these limitations through a novel, point-wise formulation of the optical transfer function (OTF) as a structured autocorrelation of the generalized pupil function (GPF). This formulation enables the estimation of phase aberrations at individual spatial coordinates with distributed computational complexity.

The research begins by developing a MATLAB-based simulation …


Algebraic Multigrid Methods For Nonsymmetric And Indefinite Problems: Theory And Applications, Ahsan Ali Jul 2025

Algebraic Multigrid Methods For Nonsymmetric And Indefinite Problems: Theory And Applications, Ahsan Ali

Mathematics & Statistics ETDs

Algebraic multigrid (AMG) is a well-established and highly efficient solver for symmetric positive definite (SPD) systems arising from elliptic and parabolic PDEs, while nonsymmetric systems from hyperbolic PDEs remain a significant challenge. This dissertation develops AMG methods and theory for nonsymmetric problems. First, we develop a novel approach combining mode constraints from energy-minimization AMG with local approximations of ideal restriction in $\ell$AIR, resulting in constrained $\ell$AIR (C$\ell$AIR), which demonstrates scalable convergence across advective and diffusive problems. Second, we extend optimal AMG theory by deriving spectral radius estimates for the two-grid error transfer operator using matrix-induced orthogonality, enabling convergence predictions for …


Learning From Conditional Data Distributions, Jizhou Huang Jul 2025

Learning From Conditional Data Distributions, Jizhou Huang

McKelvey School of Engineering Graduate Student Theses & Dissertations

Traditional machine learning paradigms often rely on a single global model trained on an entire dataset, aiming for broad generalization across all instances. However, in many real-world applications, the underlying data distribution is heterogeneous, and meaningful predictions often require models that focus on specific subpopulations rather than treating the data as a whole. This motivates the study of learning from conditional distributions, a framework where predictive models are designed to capture the structure and properties of restricted subsets of the data, leading to improved accuracy, fairness, and interpretability. This dissertation explores three key subproblems that exemplify different aspects of learning …


Gene Regulatory Network Prediction Using Machine Learning, Deep Learning, And Hybrid Approaches, Sai Teja Mummadi, Md Khairul Islam, Victor Busov, Hairong Wei Jul 2025

Gene Regulatory Network Prediction Using Machine Learning, Deep Learning, And Hybrid Approaches, Sai Teja Mummadi, Md Khairul Islam, Victor Busov, Hairong Wei

Michigan Tech Publications

Construction of gene regulatory networks (GRNs) is essential for elucidating the regulatory mechanisms underlying metabolic pathways, biological processes, and complex traits. In this study, we developed and evaluated machine learning, deep learning, and hybrid approaches for constructing GRNs by integrating prior knowledge and large-scale transcriptomic data from Arabidopsis thaliana, poplar, and maize. Among these, hybrid models that combined convolutional neural networks and machine learning consistently outperformed traditional machine learning and statistical methods, achieving over 95% accuracy on the holdout test datasets. These models not only identified a greater number of known transcription factors regulating the lignin biosynthesis pathway but also …


Network Intelligence For Next-Generation Wireless Networks: Advancing Distribution And Coordination, Yonatan Melese Worku Jul 2025

Network Intelligence For Next-Generation Wireless Networks: Advancing Distribution And Coordination, Yonatan Melese Worku

Electrical and Computer Engineering ETDs

Next-generation wireless networks, encompassing 6G and beyond, face rigorous demands for ultra-low latency, ubiquitous connectivity, exceptionally high data rates, and robust security, necessitating innovative approaches to resource optimization and network protection. This dissertation proposes a pioneering framework that synergizes advanced methodologies—deep reinforcement learning, deep learning, blockchain, and multi-agent systems—to address these challenges. Distributed architectures, underpinned by AI-driven multi-agent systems, form the backbone of this framework, enabling seamless integration and intelligent orchestration across diverse domains. The research advances IoT-based systems leveraging machine learning for resource efficiency in healthcare applications, develops reinforcement learning-driven frameworks to optimize energy and coverage for Unmanned Aerial …


In Silico Prediction Of Cytotoxic T-Cell Epitopes From Helicobacter Pylori Virulence Factors Using An Immunoinformatics Approach, Demy Valerie Chacon, Kiana Alika Co, Daphne Noreen Enriquez, Aubrey Love Labarda, Reanne Eden Manongsong, Edward Kevin B. Bragais Jul 2025

In Silico Prediction Of Cytotoxic T-Cell Epitopes From Helicobacter Pylori Virulence Factors Using An Immunoinformatics Approach, Demy Valerie Chacon, Kiana Alika Co, Daphne Noreen Enriquez, Aubrey Love Labarda, Reanne Eden Manongsong, Edward Kevin B. Bragais

Biology Faculty Publications

Background: Helicobacter pylori infects approximately half of the global population, leading to gastric and duodenal ulcers. Despite the availability of antibiotics, challenges such as patient reluctance, high treatment costs, and antibiotic resistance limit their effectiveness, making vaccination a promising alternative. This study used immunoinformatics to identify candidate epitopes for a multiepitope vaccine construct against H. pylori.

Material and methods: The protein variability server was utilized for conservation analysis. The epitopes were screened for antigenicity, allergenicity, toxicity, cross-reactivity, and population coverage. Selected epitopes were docked with their corresponding human leukocyte antigen (HLA) alleles, and thermodynamic quantities were determined. Five virulence …