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New Methods For Trace Analysis Of Iodine And Perchlorate In Biological Fluids, Charles Phillip Shelor

Chemistry & Biochemistry Dissertations - Archive

Iodine is an essential element necessary for the production of the thyroid hormones T3 and T4. These hormones are necessary for metabolism and are especially important for brain development in infants and young children. Iodine is transported into the thyroid by the sodium iodide symporter. Perchlorate is known to inhibit iodine uptake into the thyroid by binding to the sodium iodide symporter, thus acting as a potential neurotoxicant for developing children.The research in this dissertation consists of two goals. The primary is the development of novel methods for measuring iodine and perchlorate and the second is performing analyses to determine …

Investigation Of Pyrrolizidine Alkaloids In Foods Using Liquid Chromatography Mass Spectrometry, Caroline Griffin

PhDs

Pyrrolizidine Alkaloids (PAs) are known plant toxins which can cause hepatic veno-occlusive disease (HVOD) in both humans and livestock when ingested. Although PAs are primarily known for their hepatotoxicity they also induce genotoxicity, carcinogenicity and pneumotoxicity. PAs are consumed through plants as food, for medicinal purposes, or as contaminants of agricultural crops. Aside from ingesting the plants directly PA exposure may occur through the consumption of honey produced by bees that visit PA-containing plants or by drinking milk produced by animals that have consumed PA-containing plants. Possible PA contamination in our food chain is a potential health risk.

To assess …

The Development Of Liquid Chromatography Mass Spectrometry Methods For The Analysis Of Cyanobacterial Toxins In Environmental Freshwaters, Brian Anthony Dineen

Theses

Cyanobacteria, also known as blue-green algae, are a common component of many freshwater and marine ecosystems and have a potential to produce several different classes of biotoxins. The most common class of cyanobacterial toxins are the microcystins (MCs) and cylindrospermopsins (CYLs). MCs are cyclic heptapeptides that display potent hepatotoxicity and are tumour promoters. CYLs are alkaloids that are cytotoxic and can cause illness and display hepatotoxic effects in animals and humans. Worldwide, these toxins have caused fatal poisonings to animals, birds and fish. Several incidents of human intoxications have been reported and the presence of these toxins in environmental waters …

Investigation Of Pyrrolizidine Alkaloids In Foods Using Liquid Chromatography Mass Spectrometry, Caroline Griffin

Theses

Pyrrolizidine Alkaloids (PAs) are known plant toxins which can cause hepatic veno-occlusive disease (HVOD) in both humans and livestock when ingested. Although PAs are primarily known for their hepatotoxicity they also induce genotoxicity, carcinogenicity and pneumotoxicity. PAs are consumed through plants as food, for medicinal purposes, or as contaminants of agricultural crops. Aside from ingesting the plants directly PA exposure may occur through the consumption of honey produced by bees that visit PA-containing plants or by drinking milk produced by animals that have consumed PA-containing plants. Possible PA contamination in our food chain is a potential health risk.

To assess …

Specific Intracellular Uptake Of Herceptin-Conjugated Cdse/Zns Quantum Dots Into Breast Cancer Cells, Seung-Jin Han, Pierson Rathinaraj, Soo-Young Park, Young-Kyoo Kim, Joon Hyung Lee, Inn-Kyu Kang, Jong-Sik Moon, Jeffrey G. Winiarz

Chemistry Faculty Research & Creative Works

Herceptin, a typical monoclonal antibody, was immobilized on the surface of CdSe/ZnS core-shell quantum dots (QDs) to enhance their specific interactions with breast cancer cells (SK-BR3). the mean size of the core-shell quantum dots (28 nm), as determined by dynamic light scattering, increased to 86 nm after herceptin immobilization. the in vitro cell culture experiment showed that the keratin forming cancer cells (KB) proliferated well in the presence of herceptin-conjugated QDs (QD-Her, 5 nmol/mL), whereas most of the breast cancer cells (SK-BR3) had died. to clarify the mechanism of cell death, the interaction of SK-BR3 cells with QD-Her was examined …

Antioxidant Potential Of Sutherlandia Frutescens And Its Protective Effects Against Oxidative Stress In Various Cell Cultures, Shakila Tobwala, Weili Fan, Connor J. Hines, William Robert Folk, Nuran Ercal

Chemistry Faculty Research & Creative Works

Sutherlandia frutescens (L.) R.Br. (SF) is a South African plant that is widely used to treat stress, infections, cancer, and chronic diseases, many of which involve oxidative stress. The aim of the study was to quantitatively assess the antioxidant potential of SF extracts in cell-free system as well as in cell lines. Dried SF vegetative parts were extracted using six different solvents, and the extracts were assessed for total phenolic and flavonoid contents, total reducing power, iron chelating capacity, and free radical scavenging power, including, scavenging of hydroxyl radicals, superoxide anions, nitric oxide, and hydrogen peroxide. We further investigated the …

A Review Of The Hyporheic Zone, Stream Restoration, And Means To Enhance Denitrification, Leanne Merill, David J. Tonjes

Technology & Society Faculty Publications

The hyporheic zone is the subsurface area below and adjacent to a stream where groundwater mixes with stream water, through vertical, lateral, and longitudinal flows. The hyporheic zone connects the stream to uplands and other terrestrial environments. It is a zone of distinct faunal communities, high biological diversity and ecological complexity, and is the site of chemical processing and transformations of ground- and stream waters. The hyporheic zone is important to the overall ecosystem ecology of the stream, and it can influence stream water chemistry. Flows, reactions, and biota in the hyporheic zone are heterogeneous and patchy, making it difficult …

Influence Of The Sea Surface Microlayer On Ozone Deposition Velocity, Kathryn Moore

Honors Theses

The biogeochemical cycles of both iodine and ozone are strongly linked to human health and global climate and are tightly coupled through chemical processes occurring in the surface ocean and troposphere. The concentration and distribution of these compounds have the potential to drive the oxidizing capacity and suite of chemical reactions occurring in the marine boundary layer. However, modeling coupled ocean-atmosphere systems involving them is challenging because of a limited understanding of the magnitude and dominant mechanisms controlling the fluxes of both species, in addition to temporally and spatially heterogeneous distributions of ozone and iodine in the sea surface and …

Theoretical And Spectroscopic Analysis Of Ch...Π Interactions Of Fluorinated Aromatic Systems, Anuradha Gayathri Akmeemana

Masters Theses

CH...π interactions are non-covalent interactions which occur in organic compounds that are important in biological and chemical sciences in many ways. Microwave spectroscopy is an important technique that can be used to detect CH...π interactions in the gas phase molecules. Previously microwave spectroscopic studies of complexes containing CH...π interactions, benzene...HCCH (Ulrich, N.W.; Seifert, N.A.; Dorris, R.E.; Peebles, R.A.; Pate, B.H.; Peebles, S.A., Phys. Chem. Chem. Phys. 2014, 16, 8886-8894) and fluorobenzene...HCCH (Ulrich, N.W.; Songer, T.S.; Peebles, R.A.; Peebles, S.A.; Seifert, N.A.; Perez, C.; Pate, B.H. Phys. Chem. Chem. Phys., 2013, 15, 18148-18154) were done at Eastern Illinois University and the …

Distinguishing Molecular Characteristics Of Aerosol Water Soluble Organic Matter From The 2011 Trans-North Atlantic Us Geotraces Cruise, A. S. Wozniak, A. S. Willoughby, S. C. Gurganus, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

The molecular characteristics of aerosol organic matter (OM) determines to a large extent its impacts on the atmospheric radiative budget and ecosystem function in terrestrial and aquatic environments, yet the OM molecular details of aerosols from different sources are not well established. Aerosol particulate samples with North American-influenced, North African-influenced, and marine (minimal recent continental influence) air mass back trajectories were collected as part of the 2011 trans-North Atlantic US GEOTRACES cruise and analyzed for their water soluble OM (WSOM) molecular characteristics using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Principal component analysis (PCA) separated the samples into …

Quantitative Mass Spectrometric Investigations Of Protein Biomarkers: Serum Thymidine Kinase 1 And Human Osteopontin, Morse Faria

Theses and Dissertations

Mass spectrometry is being increasingly used in biomarker research mainly due to its ability to achieve high selectivity coupled with high sensitivity. This dissertation focuses on quantitative mass spectrometric investigations of two protein biomarkers i.e. serum thymidine kinase 1 (TK1) and human osteopontin (OPN).

First part of this research was focused on developing a liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method for measuring the activity of TK1 in serum by monitoring the conversion of a TK1 specific exogenous substrate, 3’-deoxy-3’-fluorothymidine (FLT), to its mono-phosphorylated form 3’-deoxy-3’-fluorothymidine monophosphate (FLT-MP). A method to quantify FLT-MP on LC-MS/MS was developed and …

Exploring The Asymmetric Environment Of Various Chiral Catalysts Using A Modified Ion-Trap Mass Spectrometer: Towards The Development Of A Rapid Chiral Catalyst Screening Method, Cary M. Davis

Theses and Dissertations

Since the tragedy of the drug Thalidomide® in the late 1950 to early 1960’s, chirality has been recognized as an important aspect that must be controlled in the drug development process in the pharmaceutical industry. Since then, there has been a considerable movement towards single enantiomer drugs. This demand has presented many challenges for the synthetic organic chemist. Chiral catalysts offer one solution to this problem, as they afford the unique ability to preferentially synthesize one enantiomer. Unfortunately, the design of new chiral catalysts is often empirical, with luck and trial and error necessary due to factors that govern enantioselectivity. …

A New And Simple Dp Polarographic Method For The Determination Of Uric Acid In Serum After Elimination Of The Interference Of Ascorbic Acid And Dopamine, Ali̇ Cengi̇z Çalişkan, Güler Somer

Turkish Journal of Chemistry

Determination of trace amounts of uric acid (UA) in serum is of great importance since its level in serum may show disorders in purine metabolism. With this method, trace amounts of UA in blood serum can be determined by a fast and simple procedure using differential pulse polarography (DPP). The limit of detection was estimated to be 2 \times 10^{-7} M (S/N = 3), lower than that given in previous studies. Among the many electrolytes studied, the best electrolyte condition was found to be 0.05 M phosphate buffer, pH 10--12, in 0.1 M KNO_3. The peak for UA appeared at …

Calixpenams: Synthesis, Characterization, And Biological Evaluation Of Penicillins V And X Clustered By Calixarene Scaffold, Fazel Nasuhi Pur, Karim Akbari Dilmaghani

Turkish Journal of Chemistry

Four 6-aminopenicillanic acid moieties were grafted at either rim of calix[4]arene, giving 2 novel generations of penicillin, which were named calixpenam. Antibiotic tests showed that they have amplified activity with respect to the corresponding penams against 3 gram-positive nonpenicillinase-producing strains of Streptococcus.

Synthesis Of Novel Imidazo[1,2-A]Pyridines And Evaluation Of Their Antifungal Activities, Füsun Göktaş, Nesri̇n Cesur, Di̇lek Şatana, Meltem Uzun

Turkish Journal of Chemistry

New 2-(imidazo[1,2-a]pyridin-2-ylcarbonyl)-N-substituted hydrazinecarbothioamides (4a--j), N'-(3-substituted-4-oxo-1,3-thiazolidin-2-ylidene)imidazo[1,2-a]pyridine-2-carbohydrazides (5a--f), and N-(nonsubstituted/4-substituted phenyl)-5-(imidazo[1,2-a]pyridine-2-yl)-1,3,4-oxadiazole-2-amines (6a--d) were synthesized from imidazo[1,2-a]pyridine-2-carbohydrazide (3) and evaluated for antifungal activity against Microsporum gypseum NCPF 580, M. canis, Trichophyton tonsurans NCPF 245, T. rubrum, Candida albicans ATCC 10231, and C. parapsilosis ATCC 22019 using amphotericin B as the standard. The chemical structures of the compounds were confirmed by elemental analysis, IR, ^1H NMR, ^{13}C NMR, HMBC (^{13}C, ^1H), and mass spectra. Most of the tested compounds showed moderate antifungal activity. Hydrazinecarbothioamide derivatives 4h and 4f exhibited the highest activity against M. canis (MIC: 2 \mu g mL^{-1} and 4 \mu g …

Antioxidant, Anticholinesterase, And Antimicrobial Activities And Fatty Acid Constituents Of Achillea Cappadocica Hausskn. Et Bornm., Abdulselam Ertaş, Mehmet Boğa, Nesri̇n Haşi̇mi̇, Yeter Yeşi̇l, Ahmet Ceyhan Gören, Gülaçti Topçu, Ufuk Kolak

Turkish Journal of Chemistry

This study was the first fatty acid analysis and biological activity report on an endemic Achillea species, Achillea cappadocica Hausskn. et Bornm. The major compounds in the fatty acid were identified as oleic (34.7 %), palmitic (23.1 %), and linoleic acids (20.6 %). The methanol and water extracts indicated higher ABTS cation radical scavenging activity than that of \alpha-tocopherol and BHT at 100 \mu g/mL. The acetone extract showed 70.62 % inhibitory activity against butyrylcholinesterase at 200 \mu g/mL. The acetone and methanol extracts exhibited moderate antimicrobial activity.

Convenient Synthesis Of New Polysubstituted Isoindole-1,3-Dione Analogues, Ayşe Tan, Mustafa Zahri̇tti̇n Kazancioğlu, Derya Aktaş, Özlem Gündoğdu, Ertan Şahi̇n, Nurhan Ki̇shali, Yunus Kara

Turkish Journal of Chemistry

Three new polysubstituted isoindole-1,3-diones were prepared from 2-ethyl-5-hydroxy-3a,4,5,7a-tetrahydro-isoindole-1,3-dione. The reaction of 2-ethyl-5-hydroxy-3a,4,5,7a-tetrahydro-isoindole-1,3-dione with m-CPBA gave the corresponding epoxide. The triacetate derivative was obtained via cis-hydroxylation using OsO_4, followed by acetylation. An aromatic derivative, a secondary reaction product, was also formed during the acetylation. Finally, a tricyclic derivative from 2-ethyl-5-hydroxy-3a,4,5,7a-tetrahydro-isoindole-1,3-dione was synthesized via dichloroketene addition under microwave irradiation. The exact structures of epoxide and tricyclic derivatives were determined by X-ray diffraction analysis.

Binding Of Flavanone With \Beta-Cd/Ctdna: A Spectroscopic Investigation, Chandrasekaran Sowrirajan, Sameena Yousuf, Muthu Vijayan Enoch Israel Vijayaraj

Turkish Journal of Chemistry

The inclusion complexation of flavanone with \beta-cyclodextrin was studied by ultraviolet absorption, steady state fluorescence, time-resolved fluorescence, and 2D ROESY nuclear magnetic resonance spectroscopic techniques. A 1:1 stoichiometric ratio was determined for the inclusion of flavanone with \beta-cyclodextrin. The Stern--Volmer constant for the accessible fraction of the binding of flavanone with \beta-cyclodextrin, and the binding constant for the flavanone--\beta-cyclodextrin complex are reported. The flavanone--\beta-cyclodextrin inclusion complex was characterized by 2D ROESY NMR spectroscopy. The binding of flavanone with ctDNA and the effect of \beta-cyclodextrin on the binding of flavanone to ctDNA were studied by absorption and fluorescence techniques. Binding constants …

Simultaneous Determination Of Dexpanthenol, Lidocaine Hydrochloride, And Mepyramine Maleate In Combined Pharmaceutical Gel By Capillary Electrophoresis, Gülden Akyil, Hayri̇ye Eda Şatana Kara, Sezen Yarimkaya Baş, Nusret Ertaş, Ni̇lgün Günden Göğer

Turkish Journal of Chemistry

A new capillary electrophoresis method was developed for the simultaneous determination of dexpanthenol (DEX), lidocaine (LID), and mepyramine (MEP) in pharmaceutical preparations. The best results were obtained using 20 mM (pH 3.0) phosphate buffer as the background electrolyte. Separation was obtained using a fused-silica capillary (75 \mu m internal diameter, 50 cm total length, 41 cm effective length) and a potential of +30 kV at 20 °C. Standards and samples were injected using a pressure injection at 50 mbar for 5 s and the analytes were monitored at a detection wavelength of 200 nm. Under optimum conditions, migration times were …

Ultrarapid Catalytic Reduction Of Some Dyes By Reusable Novel Erythromycin-Derived Silver Nanoparticles, Yasmeen Junejo, Abdulhadi̇ Baykal

Turkish Journal of Chemistry

A novel green approach for the synthesis of silver nanoparticles using erythromycin as a reducing/capping agent is presented. Erythromycin-derived silver nanoparticles were characterized by ultraviolet-visible spectroscopy, scanning electron microscopy, high resolution transmission electron microscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy. Monodispersed silver nanoparticles showed excellent and promising catalytic activity for reduction of 3 differently charged dyes (eosin B, methylene blue, and rose bengal) in the presence of NaBH_4. The study revealed that 100% reduction of these dyes can be achieved efficiently in just 150--250 s. They were easily recovered from the reaction medium and were reused 5 times, …

Rosmarinic Acid: A Potent Carbonic Anhydrase Isoenzymes Inhibitor, Meryem Topal, İlhami̇ Gülçi̇n

Turkish Journal of Chemistry

Rosmarinic acid is a water-soluble ester of caffeic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of rosmarinic acid on carbonic anhydrase isoenzymes I and II (hCA I and II) purified from human erythrocytes by using Sepharose-4B affinity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold purification of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both …

Synthesis, Characterization, And Photophysical And Photochemical Properties Of 3-(4-Phenyloxy)Coumarin Containing Metallo- And Metal-Free Phthalocyanines, Nurullah Kartaloğlu, Ali̇ye Asli Esenpinar, Mustafa Bulut

Turkish Journal of Chemistry

The synthesis of novel zinc, cobalt, indium, and metal-free phthalocyanines carrying four 3-(4-phenyloxy)couma- rins in the periphery/nonperiphery were prepared by cyclotetramerization of 3-[4-(3,4-dicyanophenyloxy)phenyl]coumarin (2)/3-[4-(2,3- dicyanophenyloxy)phenyl]coumarin (3). The novel chromogenic compounds were characterized by elemental analysis, ^1H NMR, mass spectra, F-IR, and UV-vis spectral data. The effects of the coumarin units on the zinc, indium, and metal-free phthalocyanine complexes (2a/3a, 2c/3c, 2d/3d) were also investigated.

Polyaniline In Situ Grafted To Graphene Sheets, Erwin P. Enriquez, Elliard Roswell S. Yanza, Geoffrey Matthew C. Tan, Christine Joy Querebilo, Armando Somintac, Arnel Salvador

Chemistry Faculty Publications

Graphene is one of the most promising allotropes of carbon with wide applications in nanotechnology. Modification of graphene by chemical means can further expand its uses. Here, liquid-exfoliated graphene is functionalized with p-aminophenyl moiety using p-nitrophenyl diazonium salts which were diazotized in situ then reduced by tin(II) chloride. The aminophenyl-graphene thus produced is further modified to form polyaniline-graphene (PANI-GNH) by in situ oxidative graft polymerization of aniline using ammonium peroxydisulfate as oxidizing agent. The properties of the PANI-GNH were compared with polyaniline/graphene blends by Raman, infrared and UV-Visible spectroscopy, and cyclic voltammetry. Indeed, PANI-GNH registered different spectrochemical and electrochemical properties …

Dj-1 And Atp13a2: Two Proteins Involved In Parkinson’S Disease, Josephat M Asiago

Open Access Dissertations

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease, affecting approximately 0.3% of the total U.S. population, and its prevalence increases with age. Two neuropathological hallmarks of PD are the loss of dopaminergic neurons in the substantia nigra pars compacta, a region in the midbrain involved in initiating and sustaining movement, and the presence of cytosolic inclusions called Lewy bodies (LBs) in various brain regions. LBs are enriched with fibrillar forms of the presynaptic protein &agr;-synuclein (aSyn). Two autosomal recessive genes implicated in familial PD are PARK9, encoding the P-type ATPase ATP13A2, a lysosomal ATPase; and …

Quantitative Structure-Activity Relationship (Qsar) Analysis Of Antileishmanial Natural Products, Jessica Ewald

Summer Community of Scholars Posters (RCEU and HCR Combined Programs)

No abstract provided.

Energy Transfer In A Discrete, Soluble Heterobimetallic Complex Containing Tb3+ And Eu3+, Nikhil Kumar Barua

Wayne State University Theses

A discrete, heterobimetallic Tb3+-Eu3+ -containing complex has been synthesized, and energy transfer was observed in the complex without using an antenna. This result represents a new way of observing lanthanide emission using lanthanide excitation in discrete, soluble complex. The occurrence of energy transfer in the mixed heterobimetallic Tb3+-Eu3+ complex was characterized using steady state luminescence spectroscopy and luminescence-decay studies. These findings are likely to be useful for fundamental mechanistic studies of energy transfer in lanthanide-containing systems.

Development Of Elisa-Based Hdac Activity Assay For Identification Of Isoform Selective Hdac Inhibitors, Geetha Padige

Wayne State University Dissertations

Cancers are one of the leading causes of death in United States, effecting roughly 20% of the population. To develop effective anti-cancer agents, vast research is in progress that targets various factors leading to cancer. One such area of cancer research is developing drugs that inhibit histone deacetylase (HDAC) proteins. HDACs are histone modifying enzymes that regulate transcription of genes. Aberrant recruitment of HDACs to the transcription factors leads to tumor onset and growth. Because of their potential anti-cancer therapeutic interest, several HDAC inhibitors are in various stages of clinical trials. SAHA and Romidepsin are FDA-approved drugs for the treatment …

Hiv-1 Rna Dimerization At Single Molecule Level, Hansini R. Mundigala

Wayne State University Dissertations

The Dimerization Initiation Sequence (DIS) is a conserved hairpin-loop motif on the 5' UTR of the HIV-1 genome. It plays an important role in genome dimerization through formation of a "kissing complex" intermediate between two homologous DIS sequences. This bimolecular kissing complex ultimately leads to the formation of an extended RNA duplex. Understanding the kinetics of this interaction is key to exploiting DIS as a possible drug target against HIV. We wish to report a novel study that makes an important contribution to understanding the dimerization mechanism of HIV-1 RNA in vitro. Our work has employed single-molecule fluorescence resonance energy …

Effects Of Statin Drugs And Tocotrienol Rich Fraction Supplementation In Chronic Hemodialysis Patients And Metabolomic Profile, Eno Latifi

Wayne State University Theses

Chronic kidney disease (CKD) is known as a heterogeneous disorder which currently is on the rise and lately has been classified as a public health issues in the United State and worldwide. CKD is an irreversible and progressive disease which can lead to kidney failure, and this is depicted by the advanced stage of the disorder when it reaches the point, that is classified as end stage of renal disease (ESRD) (Stage 5 of CKD) (eGRF <15 mL/min/ 1.73 m2 working capacity), where both organs are in a total or permanent kidney failure. End-Stage renal disease patients, on hemodialysis have been associated to experience an accelerated form of atherosclerosis, which is induced by inflammation, impairment of antioxidant system and elevated oxidative stress. Since the problem effecting ESRD patients is multifactorial, the objective of this investigation is to explore and look at the effects of supplementing with vitamin E-tocotrienol rich fraction (TRF), a micronutrient which has anti-inflammatory, antioxidant, and lipid lower capabilities into tackling these comorbid conditions experienced by this population. Therefore the aims of this investigation will be to explore changes in lipid profiles, inflammatory markers, and oxidative status, as well as look at any changes in metabolomic profiles. It was hypothesized that by supplementing with TRF a vitamin E, for 16 weeks in ESRD patients undergoing hemodialysis, it may help reverse and/or improve, oxidative status, inflammatory markers, increase antioxidants status and improve lipid profiles.

The study was double-blinded, randomized, parallel, placebo-controlled design trial, of 81 adult patients undergoing chronic hemodialysis at Great Lake Dialysis Clinic, Detroit MI, where …

Design And Study Of The Efflux Function Of The Egfp Fused Mexab-Oprm Membrane Transporter In Pseudomonas Aeruginosa Using Spectroscopy, Feng Ding, Kerry J. Lee, Ardeschir Vahedi-Faridi, Hiroshi Yoneyama, Christopher J. Osgood, Xiao-Hong Nancy Xu

Biological Sciences Faculty Publications

Multidrug membrane transporters (efflux pumps) can selectively extrude a variety of structurally and functionally diverse substrates (e.g., chemotoxics, antibiotics), leading to multidrug resistance (MDR) and ineffective treatment of a wide variety of diseases. In this study, we have designed and constructed a fusion gene (egfp-mexB) of N-terminal mexB with C-terminal egfp, inserted it into a plasmid vector (pMMB67EH), and successfully expressed it in the Δ MexB (MexB deletion) strain of Pseudomonas aeruginosato create a new strain that expresses MexA-(EGFP-MexB)-OprM. We characterized the fusion gene using gel electrophoresis and DNA sequencing, and determined its expression in live …