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Reduced Activity Of Multiple Endocrine Disrupting Compounds: 17Β-Estradiol And Bisphenol A With A Chemi-Luminescent Assay, Anna C. Ortiz^, Roberto J. De La Torre-Roche, Marc B. Cox, Wen-Yee Lee* Mar 2011

Reduced Activity Of Multiple Endocrine Disrupting Compounds: 17Β-Estradiol And Bisphenol A With A Chemi-Luminescent Assay, Anna C. Ortiz^, Roberto J. De La Torre-Roche, Marc B. Cox, Wen-Yee Lee*

COURI Symposium Abstracts, Spring 2011

The concern about the impacts of endocrine disrupting compounds (EDCs) in the environment has increased recently with their high occurrence in surface waters and sex changes in various aquatic organisms. EDCs include ubiquitously used pharmaceuticals and personal care products, such as soap, detergents, contraceptives, and other medicines, which are released constantly into the environment, in waste water treatment plant effluent. Though toxicological effects of these individual, EDCs have been studied, knowledge on how these chemicals interact together in the environment is very limited. The objective of this project is to study the multi-residue effect of EDCs using a chemi-luminescent yeast …

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A Novel Use Of Digoxin Immune Fab Fragment In Identification And Isolation Of An Endogenous Digitalis-Like Factor Found In Preeclampsia, Moana Lee Hopoate-Sitake Mar 2011

A Novel Use Of Digoxin Immune Fab Fragment In Identification And Isolation Of An Endogenous Digitalis-Like Factor Found In Preeclampsia, Moana Lee Hopoate-Sitake

Theses and Dissertations

The mechanisms mediating the hypertension of preeclampsia (PE) are unclear. Endogenous digitalis-like factors (EDLFs) are specific sodium pump (SP) inhibitors implicated in essential and experimental hypertension, but they have not been fully explored in the setting of PE. This study uses a digoxin antibody Fab fragment to address the question of whether such factors are present and increased in PE, to investigate a possible treatment of PE, and to isolate and characterize all EDLFs present in PE. Sera and placenta from women with PE did show a significant increase in SP inhibition in comparison to women with normal pregnancy and …

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Surface Modification, Fabrication, And Characterization Of Silicon, Polymer, And Nanotube Composite Materials, Lei Pei Mar 2011

Surface Modification, Fabrication, And Characterization Of Silicon, Polymer, And Nanotube Composite Materials, Lei Pei

Theses and Dissertations

In my research, I have performed many characterization and fabrication experiments that are based on tools of analytical chemistry, materials chemistry, and surface science. My research projects are as follows. (1) Fabrication of transparent polymer templates for nanostructured amorphous silicon photovoltaics was done using low-cost nanoimprint lithography of polydimethylsiloxane. This approach provides a test bed for absorption studies in nanostructured film geometries and should result in improved light capturing designs in thin-film solar cells. Nanopatterned polymer films were characterized by scanning electron microscopy and optical measurements. (2) A straightforward method for fabricating freely suspended, thin, carbon nanotube (CNT) membranes infiltrated …

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Transformation Of Fusarium Verticillioides With A Polyketide Gene Cluster Isolated From A Fungal Endophyte Activates The Biosynthesis Of Fusaric Acid, Yunxuan Xie, Wei Zhang, Yaoyao Li, Mingzi Wang, Ronald Cerny, Yue Shen, Liangcheng Du Mar 2011

Transformation Of Fusarium Verticillioides With A Polyketide Gene Cluster Isolated From A Fungal Endophyte Activates The Biosynthesis Of Fusaric Acid, Yunxuan Xie, Wei Zhang, Yaoyao Li, Mingzi Wang, Ronald Cerny, Yue Shen, Liangcheng Du

Liangcheng Du Publications

A large number of bioactive natural products have been isolated from plant endophytic fungi. However, molecular mechanisms for the biosynthesis of these metabolites have lagged behind because genetic and biochemical studies are difficult to perform within many of the endophytes. In this work, we describe our attempt to express a putative mycoepoxydiene (MED) biosynthetic gene cluster in Fusarium verticillioides, which has a well-developed genetic system for the study fungal polyketide biosynthesis. MED was isolated from Phomopsis sp. A123, a fungal endophyte of the mangrove plant, Kandelia candel. It has several unusual structural features and interesting biological activities. Integration of …

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Quantifying The Energetic Contributions Of Desolvation And Π-Electron Density During Translesion Dna Synthesis, Edward A. Motea, Irene Lee, Anthony J. Berdis Mar 2011

Quantifying The Energetic Contributions Of Desolvation And Π-Electron Density During Translesion Dna Synthesis, Edward A. Motea, Irene Lee, Anthony J. Berdis

Chemistry Faculty Publications

This report examines the molecular mechanism by which high-fidelity DNA polymerases select nucleotides during the replication of an abasic site, a non-instructional DNA lesion. This was accomplished by synthesizing several unique 5-substituted indolyl 2′-deoxyribose triphosphates and defining their kinetic parameters for incorporation opposite an abasic site to interrogate the contributions of π-electron density and solvation energies. In general, the Kd, app values for hydrophobic non-natural nucleotides are ∼10-fold lower than those measured for isosteric hydrophilic analogs. In addition, kpol values for nucleotides that contain less π-electron densities are slower than isosteric analogs possessing higher degrees of π-electron density. The differences …

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The Ultrafast Photoisomerizations Of Rhodopsin And Bathorhodopsin Are Modulated By Bond Length Alternation And Hoop Driven Electronic Effects, Igor Schapiro, Mikhail Nikolaevich Ryazantsev, Luis Manuel Frutos, Nicolas Ferre, Roland Lindh, Massimo Olivucci Mar 2011

The Ultrafast Photoisomerizations Of Rhodopsin And Bathorhodopsin Are Modulated By Bond Length Alternation And Hoop Driven Electronic Effects, Igor Schapiro, Mikhail Nikolaevich Ryazantsev, Luis Manuel Frutos, Nicolas Ferre, Roland Lindh, Massimo Olivucci

Chemistry Faculty Publications

Rhodopsin (Rh) and bathorhodopsin (bathoRh) quantum-mechanics/molecular-mechanics models based on ab initio multiconfigurational wave functions are employed to look at the light induced pi-bond breaking and reconstitution occurring during the Rh -> bathoRh and bathoRh -> Rh isomerizations. More specifically, semiclassical trajectory computations are used to compare the excited (S-1) and ground (S-0) state dynamics characterizing the opposite steps of the Rh/bathoRh photochromic cycle during the first 200 fs following photoexcitation. We show that the information contained in these data provide an unprecedented insight into the sub-picosecond pi-bond reconstitution process which is at the basis of the reactivity of the protein …

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Department Of Chemistry News For Alumni And Friends, March 2011, Illinois State University, Department Of Chemistry Mar 2011

Department Of Chemistry News For Alumni And Friends, March 2011, Illinois State University, Department Of Chemistry

Chem Letter

Newsletter of the Illinois State University Department of Chemistry

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Formation And Stability Of Atmospherically Relevant Isoprene-Derived Organosulfates And Organonitrates, Adam I. Darer, Neil C. Cole-Filipiak, Alison E. O'Connor, Matthew J. Elrod Mar 2011

Formation And Stability Of Atmospherically Relevant Isoprene-Derived Organosulfates And Organonitrates, Adam I. Darer, Neil C. Cole-Filipiak, Alison E. O'Connor, Matthew J. Elrod

Faculty & Staff Scholarship

Isoprene is the precursor for number of alcohol, organosulfate, and organonitrate species observed in ambient secondary organic aerosol (SOA). Recent laboratory and field work has suggested that isoprene-derived epoxides may be crucial intermediates that can explain the existence of these compounds in SOA. To confirm this hypothesis, the specific hydroxy epoxides observed in gas phase isoprene photooxidation experiments (as well as several other related species) were synthesized and the bulk phase aqueous reactions of these species in the presence of sulfate and nitrate were studied via nuclear magnetic resonance (NMR) techniques. The results indicate that both primary and tertiary organosulfates …

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Aspires At Georgia Southern University, Christine R. Whitlock, Bret S. Danilowicz, Gus J. Molina, Lisa S. Vance Mar 2011

Aspires At Georgia Southern University, Christine R. Whitlock, Bret S. Danilowicz, Gus J. Molina, Lisa S. Vance

Chemistry: Faculty Presentations (1990-2022)

Advisement and Scholarship Promoting Inquiry-based Research Experiences in STEM (ASPIRES) at Georgia Southern University is a comprehensive, strategic project that will increase the number of students graduating with bachelor's degrees in STEM fields. Five action items address two interrelated goals: (1) to increase retention of STEM students and (2) to improve academic success in science and math courses. The project ended in 2012.

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Synthesis Of Hydroxy- And Polyhydroxy-Substituted 1,3-Diaminocyclohexanes, Anobick Sar, Sergey V. Lindeman, William A. Donaldson Mar 2011

Synthesis Of Hydroxy- And Polyhydroxy-Substituted 1,3-Diaminocyclohexanes, Anobick Sar, Sergey V. Lindeman, William A. Donaldson

Chemistry Faculty Research and Publications

The synthesis of hydroxy-trans-1,3-diaminocyclo­hexanes based on nitroso-Diels-Alder cycloaddition of (cyclohexadienyl)phthalimide is reported.

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Influence Of Electric Field On The Adsorption And Bioactivity Of Hemoglobin On A Macroporous Gold Electrode, Cui-Hong Wang, Yi-Bing Kong, Xing-Hua Xia Feb 2011

Influence Of Electric Field On The Adsorption And Bioactivity Of Hemoglobin On A Macroporous Gold Electrode, Cui-Hong Wang, Yi-Bing Kong, Xing-Hua Xia

Journal of Electrochemistry

The adsorption and bioactivity of proteins at interfaces have been widely studied due to their important role in the construction of biosensors,bioelectronics and biofuel cells.Interfacial electric field is one of the important factors which could affect the adsorption and bioactivity of proteins at materials surfaces.It could dramatically change the adsorption density,molecular conformation and orientation at material surfaces.In this paper,the influence of interfacial electric field on the adsorption kinetics and bioactivity of hemoglobin(Hb) on a three-dimensional(3D) macroporous gold electrode surface has been studied using electrochemical methods and infrared spectroscopy.It was found that the interfacial electric field created excess surface charge which …

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Electrocatalytic Oxidation Of Hydrazine At Rutin Carbon Nanotubes Modified Electrode, Hong-Fang Zhang, Qing-Lin Sheng, Jian-Bin Zheng Feb 2011

Electrocatalytic Oxidation Of Hydrazine At Rutin Carbon Nanotubes Modified Electrode, Hong-Fang Zhang, Qing-Lin Sheng, Jian-Bin Zheng

Journal of Electrochemistry

The electrochemical behavior and electrocatalytic oxidation of hydrazine on rutin multiwall carbon nanotubes modified glassy carbon electrode were studied by cyclic voltammetry.The experimental results indicated that the electrode exhibits good electrocatalytic activity to hydrazine at a reduced oxidation potential of 262 mV.The amperometric response of the modified electrode showed linear increase after successive addition of hydrazine in the concentration range of 2.5×10-6~1.0×10-4 mol·L-1 with a detection limit of 5×10-7 mol·L-1.

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Development Of Monolithic Stationary Phases For Cation-Exchange Capillary Liquid Chromatography Of Peptides And Proteins, Xin Chen Feb 2011

Development Of Monolithic Stationary Phases For Cation-Exchange Capillary Liquid Chromatography Of Peptides And Proteins, Xin Chen

Theses and Dissertations

This dissertation focuses on the preparation of polymeric monolithic capillaries for ion exchange chromatography of peptides and proteins, since polymeric monoliths have shown promise for providing improved protein separations. Characteristics of monolithic columns include low back pressure, simplicity of fabrication and biocompatibility. Preparation of strong and weak cation-exchange monolithic stationary phases in 75 μm I.D. capillaries by direct in situ copolymerization was achieved using various functional monomers including sulfopropyl methacrylate, phosphoric acid 2-hydroxyethyl methacrylate, bis[2-(methacryloyloxy)ethyl] phosphate and 2-carboxyethyl acrylate with polyethylene glycol diacrylate and other PEG materials. The resulting monoliths provided excellent ion exchange capillary LC of peptides and proteins …

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Fabrication Of Covalently Crosslinked And Amine-Reactive Microcapsules By Reactive Layer-By-Layer Assembly Of Azlactone-Containing Polymer Multilayers On Sacrificial Microparticle Templates, Eric M. Saurer, Ryan M. Flessner, Maren E. Buck, David M. Lynn Feb 2011

Fabrication Of Covalently Crosslinked And Amine-Reactive Microcapsules By Reactive Layer-By-Layer Assembly Of Azlactone-Containing Polymer Multilayers On Sacrificial Microparticle Templates, Eric M. Saurer, Ryan M. Flessner, Maren E. Buck, David M. Lynn

Chemistry: Faculty Publications

We report on the fabrication of covalently crosslinked and amine-reactive hollow microcapsules using 'reactive' layer-by-layer assembly to deposit thin polymer films on sacrificial microparticle templates. Our approach is based on the alternating deposition of layers of a synthetic polyamine and a polymer containing reactive azlactone functionality. Multilayered films composed of branched poly(ethylene imine) (BPEI) and poly(2-vinyl-4,4-dimethylazlactone) (PVDMA) were fabricated layer-by-layer on the surfaces of calcium carbonate and glass microparticle templates. After fabrication, these films contained residual azlactone functionality that was accessible for reaction with amine-containing molecules. Dissolution of the calcium carbonate or glass cores using aqueous ethylenediaminetetraacetic acid (EDTA) or …

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Polycationic Glycosides, Robert Engel, Ishrat Ghani, Diego Montenegro, Marie Thomas, Barbara Klaritch-Vrana, Alejandra Castaño, Laura Friedman, Jay Leb, Leah Rothman, Heidi Lee, Craig Capodiferro, Daniel Ambinder, Eve Cere, Christopher Awad, Faiza Sheikh, Jaimelee Rizzo, Lisa-Marie Nisbett, Erika Testani, Karin Melkonian Feb 2011

Polycationic Glycosides, Robert Engel, Ishrat Ghani, Diego Montenegro, Marie Thomas, Barbara Klaritch-Vrana, Alejandra Castaño, Laura Friedman, Jay Leb, Leah Rothman, Heidi Lee, Craig Capodiferro, Daniel Ambinder, Eve Cere, Christopher Awad, Faiza Sheikh, Jaimelee Rizzo, Lisa-Marie Nisbett, Erika Testani, Karin Melkonian

Publications and Research

Cationic lipids have long been known to serve as antibacterial and antifungal agents. Prior efforts with attachment of cationic lipids to carbohydrate-based surfaces have suggested the possibility that carbohydrate-attached cationic lipids might serve as antibacterial and antifungal pharmaceutical agents. Toward the understanding of this possibility, we have synthesized several series of cationic lipids attached to a variety of glycosides with the intent of generating antimicrobial agents that would meet the requirement for serving as a pharmaceutical agent, specifically that the agent be effective at a very low concentration as well as being biodegradable within the organism being treated. The initial …

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Investigations Into Late Transition Metal Pincer Complexes As Possible Direct Partial Oxidation Catalysts, Raymond B. Lansing Jr. Feb 2011

Investigations Into Late Transition Metal Pincer Complexes As Possible Direct Partial Oxidation Catalysts, Raymond B. Lansing Jr.

Chemistry and Chemical Biology ETDs

This investigation examined aspects of a potential catalytic route for the partial oxidation of organic substrates using molecular oxygen as the oxidant. The proposed cycle begins with the insertion of molecular oxygen into a metal hydride bond to generate a metal hydroperoxide. The next step involves the transfer of an oxygen atom to an acceptor substrate to generate an oxidized species and a metal hydroxide. The starting metal hydride can then be regenerated in two viable ways, either through use of CO or H2. The experimental plan involved the syntheses and characterization of new inorganic and organometallic complexes, …

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Studies On Group 10 Pincer Carbene Metal Complexes, Agnes Mrutu Feb 2011

Studies On Group 10 Pincer Carbene Metal Complexes, Agnes Mrutu

Chemistry and Chemical Biology ETDs

Selective oxidation chemistry is a topic of intense interest worldwide, in particular when using molecular oxygen (O2) directly as the oxidizing species. The primary reason for using O2 rather than other oxidants is to take advantage of the inexpensive nature of oxygen; however, the radical ground state of O2 leads to non-selective reactions with organic substrates. As such, basic knowledge and understanding involving the fundamental reactions of O2 with organometallic species will be required in order to overcome this lack of selectivity. The work presented in this dissertation has two main research goals to help …

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Studies Of A Cyanine-Based Biosensor And Light-Induced Antibacterial Activities Of Oligo(Phenylene Ethynylene)S, Zhijun Zhou Feb 2011

Studies Of A Cyanine-Based Biosensor And Light-Induced Antibacterial Activities Of Oligo(Phenylene Ethynylene)S, Zhijun Zhou

Chemistry and Chemical Biology ETDs

This dissertation has been focusing on two subjects: biosensor development and light-activated antimicrobials. A cyanine-based fluorescent biosensor is developed with high sensitivity to detect the presence and activity of caspase-3/7. We demonstrated that supramolecular self-assembly can be useful for designing biosensors. A series of p-Phenylene Ethynylenes (OPEs) have been synthesized. Further photophysical studies show that these molecules have good singlet oxygen yields. The antimicrobial capability increases dramatically when exposed to UV-365 radiation, though dark biocidal activity can be obtained as well. This phenomenon is probably due to the high yields of singlet oxygen of these OPEs, which oxidizes unsaturated membrane …

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Human Mitochondrial Hotdog-Fold Thioesterase: Hthem4 And Hthem5, Hong Zhao Feb 2011

Human Mitochondrial Hotdog-Fold Thioesterase: Hthem4 And Hthem5, Hong Zhao

Chemistry and Chemical Biology ETDs

Hotdog-fold acyl-CoA thioesterases (ACOTs) are found in all three kingdoms of life (Archaea, Bacteria and Eukarya). All the hotdog-fold thioesterases share common catalytic scaffold and function: hydrolysis of acyl-CoA or acyl-ACP (acyl carrier protein). However the biological roles that they perform vary greatly, different substrate specificity and catalytic efficiency assign unique set of biological functions to each of them, which is further defined by tissue distribution, cellular location, protein partners and regulators. The focuses of my doctoral studies, described in this dissertation, are two members of the human hotdog-fold thioesterase superfamily: human THEM4 and human THEM5. The genes encoding hTHEM4 …

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Divergence Of Thioesterase Function: Human Bfit2, Escherichia Coli Enth, And Ydii, Danqi Chen Feb 2011

Divergence Of Thioesterase Function: Human Bfit2, Escherichia Coli Enth, And Ydii, Danqi Chen

Chemistry and Chemical Biology ETDs

My doctoral research primarily focuses on two hotdog-fold thioesterases, EntH (also known as YbdB) from E. coli, and BFIT2 from Homo sapiens. The EntH (YbdB) gene is included in a large gene cluster that encodes the enzymes of the biosynthetic pathway leading to enterobactin. Building on the hypothesis that EntH might function in a house-keeping' role by liberating misacylated EntB, two potential pathways to EntB misacylation were identified, one involving the phosphopantetheinyl transferase EntD and the other involving 2,3-DHB-AMP ligase EntE. EntH displays thioesterase activity towards a variety of acyl and aryl-holo EntB adducts. Lastly, It was shown that EntF …

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The Nature And Kinetic Analysis Of Carbon-Carbon Bond Fragmentation Reactions Of Cation Radicals Derived From Set-Oxidation Of Lignin Model Compounds, Adam S. Pimentel Feb 2011

The Nature And Kinetic Analysis Of Carbon-Carbon Bond Fragmentation Reactions Of Cation Radicals Derived From Set-Oxidation Of Lignin Model Compounds, Adam S. Pimentel

Chemistry and Chemical Biology ETDs

The lignin peroxidase promoted α-carbon β-carbon bond cleavage of two diastereomeric pairs of dimeric lignin model compounds were investigated to determine which structural units are more readily cleaved. These model compounds, β-1 (1,2-diaryl-1,3-propanediol) and β-O-4 (1-diaryl-2-aryloxy-1,3-propanediol), represent the most common structural units present in the plant cell wall polymer lignin. The lignin peroxidase catalyzed reaction mechanism was shown to parallel two mechanistically well understood systems, the cerric (IV) ammonium nitrate promoted chemical reacton, and the dicyannoanthracene promoted photochemical reaction, both of which proceed by a SET mechanism. Product profiles and kinetic rate constants were determined and compared for all four …

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Reciprocal Regulation Of Trek-1 Channels By Arachidonic Acid And Crh In Mouse Corticotropes, Andy K. Lee, James L. Smart, Marcelo Rubinstein, Malcolm J. Low, Amy Tse Feb 2011

Reciprocal Regulation Of Trek-1 Channels By Arachidonic Acid And Crh In Mouse Corticotropes, Andy K. Lee, James L. Smart, Marcelo Rubinstein, Malcolm J. Low, Amy Tse

Faculty Publications - Department of Biological & Molecular Science

Arachidonic acid (AA) is generated in the anterior pituitary gland upon stimulation by the ACTH secretagogue, CRH. Using the patch clamp technique,we examined the action of AA on the excitability of single pituitary corticotropes obtained from a transgenic mouse strain that expresses the enhanced green fluorescent protein driven by the proopiomelanocortin promoter. CRH evoked depolarization, but AA caused hyperpolarization. Under voltage clamp condition, AA caused a rapid inhibition of the delayed rectifier K current and then increased a background K current. Inhibition of AA metabolism did not prevent the activation of the K current by AA, suggesting a direct action …

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Growth Factor Signaling Enhances Aromatase Activity Of Breast Cancer Cells Via Post-Transcriptional Mechanisms, Bin Su, Cynthie Wong, Yanyan Hong, Shiuan Chen Feb 2011

Growth Factor Signaling Enhances Aromatase Activity Of Breast Cancer Cells Via Post-Transcriptional Mechanisms, Bin Su, Cynthie Wong, Yanyan Hong, Shiuan Chen

Chemistry Faculty Publications

It has been demonstrated that growth factors produced by breast cancer cells stimulate aromatase expression in both breast cancer and adjacent adipose fibroblasts and stromal cells. However, whether these growth factors affect aromatase activity by other mechanisms still remain unclear. In the current study, MCF-7aro and T47Daro aromatase transfected breast carcinoma cells were used to explore the mechanisms of post-transcriptional regulation of aromatase activity by growth factor pathways. Our study reveals that PI3K/Akt and MAPK inhibitors suppressed aromatase activity in MCF-7aro cells. However, PI3K/Akt pathway inhibitors stimulated aromatase activity in T47Daro cells. This is due to enhanced MAPK phosphorylation as …

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Methods And Compositions For Treating Neurological Disorders, Tae H. Ji, Inhae Ji Jan 2011

Methods And Compositions For Treating Neurological Disorders, Tae H. Ji, Inhae Ji

Chemistry Faculty Patents

This disclosure relates to methods and compositions useful for the treatment of senile dementia. More particularly the disclosure relates to methods and compositions for the treatment of senile dementia related to diabetes.

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Electrochemical Studies Of Quinone On Clay-Modified Platinum Electrodes, Thomas Edward Nelson Jan 2011

Electrochemical Studies Of Quinone On Clay-Modified Platinum Electrodes, Thomas Edward Nelson

Theses (2 year embargo)

No abstract provided.

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Spectroscopic And Computational Insight Into Weak Noncovalent Interactions In Crystalline Pyrimidine, Ashley M. Wright, Lynn V. Joe, Austin A. Howard, Gregory S. Tschumper, Nathan I. Hammer Jan 2011

Spectroscopic And Computational Insight Into Weak Noncovalent Interactions In Crystalline Pyrimidine, Ashley M. Wright, Lynn V. Joe, Austin A. Howard, Gregory S. Tschumper, Nathan I. Hammer

Chemistry Faculty Research & Creative Works

Effects of weak C-H⋯N interactions on the vibrational spectrum of crystalline pyrimidine are studied using Raman spectroscopy and electronic structure computations. When pyrimidine transitions from the liquid to solid state, peaks in the Raman spectrum exhibit non-uniform shifts. Calculations performed on a cluster of nine pyrimidine molecules (one pyrimidine molecule embedded in a field of its eight nearest neighbors in the crystalline geometry) predict that modes involving the movement of hydrogen atoms are associated with the observed shifts. Assignments of fundamentals and combination bands in both the liquid and solid states are also revised in the complicated C-H stretching region. …

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A P38Α-Selective Chemosensor For Use In Unfractionated Cell Lysates, Cliff I. Stains, Elvedin Luković, Barbara Imperiali Jan 2011

A P38Α-Selective Chemosensor For Use In Unfractionated Cell Lysates, Cliff I. Stains, Elvedin Luković, Barbara Imperiali

Cliff Stains Publications

Recent efforts have identified the p38α Ser/Thr kinase as a potential target for the treatment of inflammatory diseases as well as non-small cell lung carcinoma. Despite the significance of p38α, no direct activity probe compatible with cell lysate analysis exists. Instead, proxies for kinase activation, such as phosphospecific antibodies, which do not distinguish between p38 isoforms, are often used. Our laboratory has recently developed a sulfonamido-oxine (Sox) fluorophore that undergoes a significant increase in fluorescence in response to phosphorylation at a proximal residue, allowing for real-time activity measurements. Herein we report the rational design of a p38α-selective chemosensor using this …

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Spin Transition In Gd3n@C-80, Detected By Low-Temperature On-Chip Squid Technique, L. Chen, E. E. Carpenter, C. S. Hellberg, H. C. Dorn, M. Schultz, W/ Wernsdorfer, I. Chiorescu Jan 2011

Spin Transition In Gd3n@C-80, Detected By Low-Temperature On-Chip Squid Technique, L. Chen, E. E. Carpenter, C. S. Hellberg, H. C. Dorn, M. Schultz, W/ Wernsdorfer, I. Chiorescu

Chemistry Publications

We present a magnetic study of the Gd3N@C80 molecule, consisting of a Gd-trimer via a nitrogen atom, encapsulated in a C80 cage. This molecular system can be an efficient contrast agent for magnetic resonance imaging (MRI) applications. We used a low-temperature technique able to detect small magnetic signals by placing the sample in the vicinity of an on-chip SQUID. The technique implemented at the National High Magnetic Field Laboratory has the particularity of being able to operate in high magnetic fields of up to 7 T. The Gd3N@C80shows a paramagnetic behavior and …

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Semen Quality And Chemical Oxidative Stress; Quantification And Remediation, Saleem Ali Bani-Hani Jan 2011

Semen Quality And Chemical Oxidative Stress; Quantification And Remediation, Saleem Ali Bani-Hani

ETD Archive

The necessary multi-step process to prepare human semen for assisted reproduction (AR) such as in vitro fertilization (IVF) is known to induce oxidative stress in human spermatozoa and subsequent damage to the integrity of their cell components and, thus, to their function. Literature reports have viewed this as a potential link with the high frequency of birth defects among the IVF babies. In this work, we studied the effect of incubation and centrifugation on human sperm quality using established AR protocols. We found that in vitro incubation and centrifugation (20 min at 220g) of sperm generally degrade sperm quality in …

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Quantitative And Qualitative Mass Spectrometric Analysis Of Anticancer Agents, Drugs Of Abuse And Enzyme-Inhibitor Complexes, Keri M. Smith Jan 2011

Quantitative And Qualitative Mass Spectrometric Analysis Of Anticancer Agents, Drugs Of Abuse And Enzyme-Inhibitor Complexes, Keri M. Smith

ETD Archive

Mass spectrometry is a valuable tool in the analysis of many types of compounds. From small molecules to large proteins, mass spectrometry can help to interrogate samples and give insights to the origins of disease as well as calculate an exact concentration. Bioanalytical method development is an integral component in the measurement of such compounds. To facilitate proper analytical investigation, the process of method development, analysis, and interpretation must be understood. This work describes the basis and procedure for bioanalytical method development and its detailed application to preclinical studies of the antineoplastic agent hexamethylene bisacetamide and measurement ofillicit drugs of …

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