Digital Commons Network^™

Characterization Of The Gas-Sensing Protein Cooa, Teryn Gehred, Jessica Lyza

Symposium on Research and Creative Expression (SORCE)

This research aims to characterize the binding specificity of heme proteins to various gas signal molecules. Heme proteins in the gas-sensing family bind to specific gas signal molecules, causing a certain biological function to be activated or deactivated. In this study, variants of the carbon monoxide sensing heme protein CooA from the bacteria R.rubrum and C. hydrogenoformans are compared. Key differences in signal binding specificity and heme environment configuration will be investigated. This is done by using site-directed mutagenesis to alter the heme environment, isolating the CooA proteins through protein purification, and analyzing the isolated products through DNA binding assays. …

Study Of Ligand Binding Reactions Of Bis-Aminemetalloporphyrins, Frank Wang

Symposium on Research and Creative Expression (SORCE)

Porphyrins are molecular complexes that are critical for the proper functioning of many important biological macromolecules such as hemoglobin and myoglobin. The goal of this research is to monitor the transformations and differences that occur with the addition of metals and ligands to a model porphyrin compound called tetraphenylporphyrin (TPP). During this work, a series of metalloporphyrin complexes were prepared, with changes to both metal centers and ligand attachments at those centers. Specifically, iron, zinc, cobalt and manganese metalloporphyrins have been synthesized and identities verified via electronic absorption spectroscopy. Ligand binding studies have begun with the addition of piperidine and …

Cristal (Chemistry Resources And Instructional Strategies For Teaching All Learners For Ms & Hs Chemistry), Jody Stone

K-12 Curriculum Supplements

The CRISTAL program was developed for the purpose of (1) increasing the number of quality laboratory experiences carried out in high school chemistry classes (2) providing learning activities designed to stimulate the development of reasoning/problem-solving skills and (3) providing chemistry teachers with ideas and strategies for creating a more student-centered classroom. The intended audience is all students enrolled in high school chemistry, especially those students needing additional motivation for learning chemistry.

The CRISTAL materials include a teacher's resource manual containing over 100 activities. These laboratories are arranged in a learning cycle format involving exploratory and application activities. One or more …

Preparation Of A Semiquinonate-Bridged Diiron(Ii) Complex And Elucidation Of Its Geometric And Electronic Structures, Amanda E. Baum, Sergey V. Lindeman, Adam T. Fiedler

Chemistry Faculty Research and Publications

The synthesis and crystal structure of a diiron(II) complex containing a bridging semiquinonate radical are presented. The unique electronic structure of this S = 7/2 complex is examined with spectroscopic (absorption, EPR, resonance Raman) and computational methods.

Persepsi Pengelola Terhadap Peran Sistem Informasi Manajemen (Sim) Berbasis Teknologi Informasi (Ti) Dalam Pengelolaan Administrasi Sekolah Menengah Kejuruan Kota Mojokerto, Tri Wahjoedi Hidajat

Jurnal Pendidikan Sains

Peran sistem informasi manajemen (SIM) berbasis teknologi informasi sangat penting dalam pengelolaan sekolah untuk meningkatkan kualitas pelayanan, proses, dan prestasi belajar siswa. Oleh karena itu, penelitian ini bertujuan untuk mengungkap dan mendeskripsikan persepsi pengelola terhadap peran SIM berbasis TI dalam pengelolaan administrasi SMK di Kota Mojokerto. Penelitian ini menggunakan rancangan deskriptif kuantitatif dan dilaksanakan pada sembilan SMK di Kota Mojokerto dengan 45 pengelola administrasi akademik sebagai populasi. Teknik pengambilan sampel yang digunakan adalah sampel jenuh, yaitu seluruh populasi dijadikan sampel. Hasil penelitian menunjukkan bahwa: (1) secara umum, persepsi pengelola terhadap peran SIM berbasis TI dalam pengelolaan administrasi SMK tergolong penting, …

Penerapan Pembelajaran Inkuiri Terbimbing Berbasis Laboratorium Untuk Meningkatkan Keterampilan Proses Dan Hasil Belajar Ipa Siswa Kelas Viii-4 Di Smpn 1 Probolinggo, Karyatin Karyatin

Jurnal Pendidikan Sains

Penelitian ini bertujuan untuk mendeskripsikan keterlaksanaan pembelajaran inkuiri terbimbing berbasis kegiatan laboratorium dalam meningkatkan keterampilan proses sains dan hasil belajar kognitif IPA (biologi) siswa. Deskripsi keterlaksanaan pembelajaran diperoleh melalui identifikasi permasalahan yang muncul selama proses pembelajaran di kelas, yang selanjutnya dianalisis untuk menemukan solusi berdasarkan data empiris di lapangan. Penelitian ini juga berupaya mengungkap permasalahan pembelajaran secara menyeluruh dan kontekstual dengan menempatkan peneliti sebagai instrumen kunci. Pendekatan yang digunakan adalah kualitatif dengan jenis penelitian tindakan kelas (PTK) yang dilaksanakan dalam dua siklus. Setiap siklus terdiri atas empat pertemuan dengan alokasi waktu 2 × 40 menit per pertemuan. Teknik pengumpulan data …

Kajian Morfologi, Anatomi, Dan Agronomi Antara Kedelai Sehat Dengan Kedelai Terserang Cowpea Mild Mottle Virus Serta Pemanfaatannya Sebagai Bahan Ajar Di Sekolah Menengah Kejuruan, As’Ad Syamsul Arifin

Jurnal Pendidikan Sains

Salah satu penyebab rendahnya produktivitas kedelai di Indonesia adalah serangan virus belang samar kacang tunggak, Cowpea mild mottle virus (CPMMV). Penelitian ini merupakan penelitian survei yang bertujuan untuk menggambarkan dan mengumpulkan informasi mengenai kajian morfologi, anatomi, dan agronomi terhadap infeksi CPMMV pada varietas yang terinfeksi dan yang tidak terinfeksi. Data dianalisis secara deskriptif dan inferensial menggunakan ANAVA melalui program SPSS for Windows versi 16. Hasil penelitian menunjukkan bahwa varietas Gumitir sehat, Anjosmoro sehat, Mahameru sehat, MLGG 0021, dan MLGG 0268 memiliki ukuran daun, panjang petiol, serta nisbah daun (L/W dan L/PL) lebih besar dibandingkan varietas yang sakit. Varietas Argopuro sakit …

Pembelajaran Kooperatif Tai Dan Game Puzzle Dalam Meningkatkan Motivasi Belajar Dan Pemahaman Konsep Matematika, Any Herawati

Jurnal Pendidikan Sains

Penelitian ini mendeskripsikan rancangan dan pelaksanaan pembelajaran kooperatif Team Assisted Individualized (TAI) dan game puzzle dalam meningkatkan motivasi belajar serta pemahaman konsep fungsi komposisi dan fungsi invers di SMA Negeri 3 Malang. Penelitian ini merupakan penelitian tindakan kelas dengan pendekatan mix research, yang dilaksanakan dalam tiga siklus. Subjek penelitian adalah siswa kelas XI IPA 1 semester genap tahun pelajaran 2011/2012 sebanyak 33 siswa, terdiri atas 13 siswa laki-laki dan 20 siswa perempuan. Data yang dikumpulkan meliputi motivasi belajar dan pemahaman konsep matematika siswa. Hasil penelitian menunjukkan bahwa pembelajaran kooperatif TAI dan game puzzle mampu meningkatkan motivasi belajar dan pemahaman konsep. …

Pembelajaran Berbantuan Multimedia Berdasarkan Teori Beban Kognitif Untuk Meningkatkan Kemampuan Menyelesaikan Masalah Program Linear Siswa X Tkr 1 Smkn 1 Doko, Fifi Damayanti

Jurnal Pendidikan Sains

Berdasarkan teori beban kognitif, pembelajaran efektif terjadi apabila beban intrinsic dikelola dengan baik, beban germane ditingkatkan, dan beban extraneous dikurangi. Penerapan multimedia merupakan salah satu upaya untuk mencapai pembelajaran yang efektif. Penelitian ini mengkaji penerapan pembelajaran matematika berbantuan multimedia dalam meningkatkan kemampuan siswa menyelesaikan masalah program linear. Penelitian ini menggunakan pendekatan penelitian tindakan kelas yang dilaksanakan dalam dua siklus. Hasil penelitian menunjukkan bahwa penggunaan multimedia dapat mengelola beban intrinsic, meningkatkan beban germane, dan mengurangi beban extraneous, sehingga kemampuan siswa dalam menyelesaikan masalah program linear meningkat.

Penggunaan Pita Melalui Pembelajaran Missouri Mathematics Project Untuk Memahamkan Siswa Tentang Luas Permukaan Bola, Pentri Arfia Dewi

Jurnal Pendidikan Sains

Penelitian ini akan mendeskripsikan penggunaan pita melalui pembelajaran Missouri Mathematics Project untuk memahamkan siswa tentang luas permukaan bola. Pembelajaran yang dilaksanakan berdasarkan pada tahapan pembelajaran Missouri Mathematics Project yang dipadukan dengan bahan manipulatif. Penelitian ini merupakan penelitian tindakan kelas. Setiap siklus terdiri dari tiga kali pertemuan. Dalam penelitian ini juga dilakukan kajian dari jawaban siswa. Hasil penelitian ini menunjukkan bahwa penerapan pembelajaran menggunakan bahan manipulatif bola dan pita dapat memahamkan siswa tentang luas permukaan bola.

Pengaruh Model Siklus Belajar Berbantuan Mind Map Terhadap Prestasi Belajar Fisika Ditinjau Dari Kinerja Laboratorium Siswa Kelas Viii Smpn 1 Rejoso Kabupaten Pasuruan, Ary Analisa Rahma

Jurnal Pendidikan Sains

Penelitian ini bertujuan untuk menguji pengaruh model siklus belajar berbantuan mind map terhadap prestasi belajar fisika ditinjau dari kinerja laboratorium siswa kelas VIII pada materi cahaya di SMP Negeri 1 Rejoso, Kabupaten Pasuruan. Penelitian ini merupakan penelitian eksperimen semu dengan rancangan faktorial 2 × 2. Subjek penelitian berjumlah 204 siswa. Instrumen penelitian terdiri atas instrumen perlakuan yang meliputi silabus, RPP, dan LKS, serta instrumen pengukuran berupa instrumen kinerja laboratorium dan tes prestasi belajar fisika. Hasil penelitian menunjukkan bahwa prestasi belajar fisika siswa yang belajar menggunakan model siklus belajar berbantuan mind map lebih tinggi dibandingkan dengan siswa yang belajar melalui pembelajaran …

Analisis Variasi Genetik Varian Jati Arboretum Dengan Penanda Mikrosatelit, Imas Cintamulya

Jurnal Pendidikan Sains

Seleksi tanaman jati untuk memperoleh sifat unggul tidak cukup hanya melalui penanda morfologi, tetapi juga perlu menggunakan penanda molekuler berdasarkan polimorfisme DNA. Oleh karena itu, perlu dilakukan kajian tanaman jati dari segi genetik pada level DNA untuk melihat variasi genetik. Informasi tentang variasi genetik jati diperlukan dalam pengadaan bibit untuk pembangunan hutan tanaman industri, dengan tujuan mencegah inbreeding dan mendukung upaya konservasi. Penelitian ini bertujuan untuk menganalisis variasi genetik varian jati arboretum menggunakan penanda molekuler mikrosatelit serta menyusun buku pengayaan kajian dasar-dasar molekuler tumbuhan, khususnya penanda mikrosatelit, dalam menganalisis variasi genetik varian jati arboretum yang berkualitas. Langkah-langkah penelitian meliputi isolasi …

Pengembangan Profesionalitas Berkelanjutan/Continuing Professional Development (Cpd) Guru Bersertifikat Pendidik Di Smk Rumpun Teknologi Se-Malang Raya, Sujianto Sujianto

Jurnal Pendidikan Sains

Penelitian ini bertujuan untuk mendeskripsikan pengembangan profesionalitas berkelanjutan guru bersertifikat pendidik melalui investasi pengembangan diri, publikasi ilmiah, dan karya inovatif, baik secara mandiri, berkelompok, maupun melembaga. Penelitian menggunakan rancangan deskriptif kuantitatif dengan subjek guru kejuruan bersertifikat pendidik di wilayah Malang Raya. Instrumen penelitian meliputi kuesioner (angket) dan pedoman wawancara. Hasil penelitian menunjukkan bahwa pemberlakuan Undang-Undang Guru dan Dosen (UU Nomor 14 Tahun 2005) yang disertai pemberian tunjangan profesi sejatinya memberikan harapan besar dalam mendorong minat guru untuk terus mengembangkan profesionalitasnya. Namun, temuan penelitian menunjukkan kondisi yang berbeda. Tingkat pengembangan profesionalitas berkelanjutan guru bersertifikat pendidik di SMK rumpun teknologi se-Malang Raya …

Integrasi Pendidikan Karakter Melalui Inkuiri Dengan Lesson Study Dalam Pembelajaran Biologi Untuk Meningkatkan Sikap Ilmiah Dan Hasil Belajar Kognitif Siswa Kelas Vii Smpn I Singosari, Elly Lailatul Budur

Jurnal Pendidikan Sains

Pembelajaran inkuiri memungkinkan siswa terlibat secara aktif dalam proses berpikir dan menemukan konsep yang dipelajarinya. Pengetahuan dan keterampilan yang diperoleh siswa diharapkan bukan sekadar hasil menghafal fakta, melainkan hasil dari proses penemuan secara mandiri. Hasil pengamatan awal menunjukkan bahwa siswa SMP Negeri 1 Singosari masih cenderung menghafal materi pembelajaran sehingga keterampilan proses dan sikap ilmiah belum berkembang secara optimal. Selain itu, karakter siswa sebagaimana diamanatkan dalam tujuan pendidikan nasional belum sepenuhnya tercapai. Penelitian ini bertujuan untuk meningkatkan sikap ilmiah dan hasil belajar kognitif siswa melalui integrasi pendidikan karakter dengan metode inkuiri terbimbing berbasis lesson study pada siswa kelas VII SMP …

Carcinogenic Adducts Induce Distinct Dna Polymerase Binding Orientations, Kyle B. Vrtis, Radoslaw P. Markiewicz, Louis J. Romano, David Rueda

Chemistry Faculty Research Publications

DNA polymerases must accurately replicate DNA to maintain genome integrity. Carcinogenic adducts, such as 2-aminofluorene (AF) and N-acetyl-2-aminofluorene (AAF), covalently bind DNA bases and promote mutagenesis near the adduct site. The mechanism by which carcinogenic adducts inhibit DNA synthesis and cause mutagenesis remains unclear. Here, we measure interactions between a DNA polymerase and carcinogenic DNA adducts in real-time by single-molecule fluorescence. We find the degree to which an adduct affects polymerase binding to the DNA depends on the adduct location with respect to the primer terminus, the adduct structure and the nucleotides present in the solution. Not only do the …

Electrochemical Nanofabrication Using Polyacrylamide Hydrogel As Soft Stamps, Wen Sun, Hai-Tao Fan, Da-Xiao Zhang, Dong-Jie Hu, Yong-Liang Zhou

Journal of Electrochemistry

The fabrication resolution of electrochemical wet stamping (E-WETS) was enhanced to several hundred nanometers from several ten micrometers by using polycarylamide gel (PAG) as soft stamps and through optimized processes. The PAG stamp was cured on a nanopatterned soft mold and soaked with 0.2 mol·L^-1 KCl solution, then contacted with a silicon wafer with gold film and applied with electric field. And the gold nanopatterns were achieved by the selective anodic dissolving, the diameter of fabricated gold spot was around 200 nm. The parameters which affected the fabrication processes have been discussed in detail.

Investigation Of Water-Molecule Complexes And Their Catalytic Effect On Important Atmospheric Reactions, Taylor Scott Cline

Theses and Dissertations

This dissertation is a collection of works that investigates issues related to environmental chemistry. The first portion of this research explores the role of water vapor on the kinetics of important atmospheric reactions. Work is presented on the self-reaction of β-hydroxyethyl peroxy radical (β-HEP) and the catalytic increase in reaction rate by water vapor. β-HEP serves as a model system for investigating the possible role of water vapor in perturbing the kinetics and product branching ratio of atmospheric reactions of other alkyl peroxy radicals. The self-reaction rate coefficient of β-HEP was investigated between 276-296 K with 1.0 × 10^15 to …

Extending Single Molecule Fluorescence Observation Time By Amplitude-Modulated Excitation, Lydia Kisley

Faculty Scholarship

We present a hardware-based method that can improve single molecule fluorophore observation time by up to 1500% and super-localization by 47% for the experimental conditions used. The excitation was modulated using an acousto-optic modulator (AOM) synchronized to the data acquisition and inherent data conversion time of the detector. The observation time and precision in super-localization of four commonly used fluorophores were compared under modulated and traditional continuous excitation, including direct total internal reflectance excitation of Alexa 555 and Cy3, non-radiative Förster resonance energy transfer (FRET) excited Cy5, and direct epi-fluorescence wide field excitation of Rhodamine 6G. The proposed amplitude-modulated excitation …

Polypeptides, Systems, And Methods Useful For Detecting Glucose, Sylvia Daunert, Kendrick Turner, Smita Joel, Laura Rowe

Chemistry Faculty Patents

The presently-disclosed subject matter provides biosensors for detecting molecules of interest. The biosensors include a polypeptide capable of selectively-binding glucose, wherein the polypeptide molecule is selected from: an unnatural analogue of wild type glucose binding protein; a fragment of wild type glucose binding protein; and an unnatural analogue fragment of wild type glucose binding protein.

Modeling Of An Ion Exchange Reaction Involving Decamethylcucurbit[5]Uril In The Gas Phase, Jacob Hedelius, Dr. David Dearden

Journal of Undergraduate Research

Mathematical modeling is both common and increasingly expected in scientific research, to both predict and to explain results. Models are used in many disciplines, from describing cellular process in biology, weather patterns in planetary science, to galaxies in physics. As part of my studies with the Office of Research and Creative Activities (ORCA), I created a simple model for reactions of the molecule decamethylcucurbit[5]uril (mc5) in the gas phase.

Identification Of Novel Ribosome Stalling Peptides Using The Tat-Two Hybrid, Benjamin Van Leeuwen, Dr. Allen Buskirk

Journal of Undergraduate Research

Dr. Buskirk discontinued my previous project on discovering novel ribosomal stalling sequences shortly after the start of the semester because of its lack of relevance to more current ribosomal research trends. While there are still an unknown number of stalling sequences that have yet to be discovered, the current research has moved away from that field into more novel ribosomal research. Therefore, I was not able to finish my project as planned and presented in my last application.

Study Of The Thermodynamics Of Chromium(Iii) And Chromium(Vi) Binding To Iron(Ii/Iii)Oxide Or Magnetite Or Ferrite And Magnanese(Ii) Iron (Iii) Oxide Or Jacobsite Or Manganese Ferrite Nanoparticles, Steven Luther, Nathan Brogfeld, Jisoo Kim, Jason Parsons

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

Removal of chromium(III) or (VI) from aqueous solution was achieved using Fe3O4, and MnFe2O4 nanomaterials. The nanomaterials were synthesized using a precipitation method and characterized using XRD. The size of the nanomaterials was determined to be 22.4±0.9 nm (Fe3O4) and 15.5±0.5 nm (MnFe2O4). The optimal binding pH for chromium(III) and chromium(VI) were pH 6 and pH 3. Isotherm studies were performed, under light and dark conditions, to determine the capacity of the nanomaterials. The capacities for the light studies with MnFe2O4 and Fe3O4 were determined to be 7.189 and 10.63 mg/g, respectively, for chromium(III). The capacities for the light studies …

Förster Resonance Energy Transfer Investigations Of The Structure Of The Immunoglobulin G Class Of Antibody, Michael Kelliher

College of Science and Health Theses and Dissertations

Ensemble and single molecule methods were used to study model systems of 11 base pair and 16 base pair oligonucleotides. From these experiments it was possible to determine the best ensemble analysis method to use, in conjunction with single molecule experiments, to investigate the structure of lgG in solution. To study the interactions within the antigen-binding fragments (Fab), the binding sites were labeled with a FRET pair. Single molecule fluorescence measurements on the antibody indicate minimal FRET occurring between the dye pair suggesting the distance is too large to be measured using single molecule FRET. Ensemble and single molecule FRET …

Miniaturized Electrostatic Ion Beam Trap Mass Analyzer, Junting Wang

Theses and Dissertations

The electrostatic ion beam trap (EIBT) was designed by D. Zajfman during the previous decade. This ion trap combines many properties of the Fourier-transform ion cyclotron resonance (FTICR) mass analyzer and time-of-flight (TOF) mass analyzer. There are several advantages for the electrostatic ion beam trap. First, large mass-to-charge particles in an electrostatic field could be easier to analyze. Second, there is a folded flight path, which could make the mass analyzer smaller compared to conventional TOF mass analyzer. This principle of operation of this ion trap is analogous to an optical resonator. The ions are trapped in a voltage valley …

Inhibition Of Fast Axonal Transport By Pathogenic Sod1 Involves Activation Of P38 Map Kinase, Gerardo Morfini, Daryl Bosco, Hannah Brown, Rodolfo Gatto, Agnieszka Kaminska, Yuyu Song, Ashutosh Tiwari, Et. Al.

Department of Chemistry Publications

Dying-back degeneration of motor neuron axons represents an established feature of familial amyotrophic lateral sclerosis (FALS) associated with superoxide dismutase 1 (SOD1) mutations, but axon-autonomous effects of pathogenic SOD1 remained undefined. Characteristics of motor neurons affected in FALS include abnormal kinase activation, aberrant neurofilament phosphorylation, and fast axonal transport (FAT) deficits, but functional relationships among these pathogenic events were unclear. Experiments in isolated squid axoplasm reveal that FALS-related SOD1 mutant polypeptides inhibit FAT through a mechanism involving a p38 mitogen activated protein kinase pathway. Mutant SOD1 activated neuronal p38 in mouse spinal cord, neuroblastoma cells and squid axoplasm. Active p38 …

Single-Step Electrochemical Functionalization Of Double-Walled Carbon Nanotube (Dwcnt) Membranes And The Demonstration Of Ionic Rectification, Xin Zhan, Ji Wu, Zhiqiang Chen, Bruce J. Hinds

Chemistry Faculty Publications

Carbon nanotube (CNT) membranes allow the mimicking of natural ion channels for applications in drug delivery and chemical separation. Double-walled carbon nanotube membranes were simply functionalized with dye in a single step instead of the previous two-step functionalization. Non-faradic electrochemical impedance spectra indicated that the functionalized gatekeeper by single-step modification can be actuated to mimic the protein channel under bias. This functional chemistry was proven by a highly efficient ion rectification, wherein the highest experimental rectification factor of ferricyanide was up to 14.4. One-step functionalization by electrooxidation of amine provides a simple and promising functionalization chemistry for the application of …

Identification And Differentiation Of Tier 1 Bacterial Agents Using Gas Chromatography-Mass Spectrometry, Dan Li

Theses and Dissertations

A simple method was developed for detection and differentiation of five Tier 1 bacterial agents, including Bacillus anthracis, Francisella tularensis, Yersinia pestis, Burkholderia pseudomallei and Burkholderia mallei as well as their closely related near neighbors by gas chromatography-mass spectrometry (GC-MS). Generally, different classes of compounds can be used as biomarkers for biowarfare agent detection, including nucleic acids (i.e., DNA or RNA), proteins (i.e., antibodies), carbohydrates (i.e., sugars), lipopolysaccharides, lipids (i.e., fatty acids) and small molecules. One-step thermochemolysis (TCM) was developed to provide GC-MS detectable biomarker signatures, including sugars, fatty acids and small molecules. Solid phase micro-extraction (SPME) was used for …

Overview Of The Recent Fda Process Validation Guidance For Medicinal Product Development And Manufacture, Nuala Calnan (Editor)

Conference Papers

This conference paper presents an overview of the recently published FDA Process Validation Guideline for the development and manufacture of medicinal drug products. It discusses the impacts of conducting life cycle based validation activities and highlights the future challenges in meeting the ongoing 'Continued Process Validation' (CPV) expectations of the regulator. It also presents the latest information on the EU draft Process Validation Guidance - due for publication in late 2013 / 2014.

Bacterial Spores Remain Viable After Electrospray Charging And Desolvation, Sara Nielson Pratt

Theses and Dissertations

The electrospray survivability of B. subtilis spores and E. coli was tested in atmospheric mobility experiments. E. coli did not survive electrospray charging and desolvation, but B. subtilis did. Experimental conditions ensured that any surviving bacteria were charged, desolvated, and de-agglomerated. B. subtilis was also found to survive both positive and negative electrospray and subsequent introduction into vacuum conditions. Attempts were made to measure the charge distribution of viable B. subtilis spores using electrostatic deflection. From those experiments, it was found that either the spores do not become highly charged under the electrospray conditions used or only spores in a …

Identification Of Cisplatin-Binding Proteins Using Agarose Conjugates Of Platinum Compounds, Takatoshi Karasawa, Martha Sibrian-Vazquez, Robert M. Strongin, Peter S. Steyger

Chemistry Faculty Publications and Presentations

Cisplatin is widely used as an antineoplastic drug, but its ototoxic and nephrotoxic side-effects, as well as the inherent or acquired resistance of some cancers to cisplatin, remain significant clinical problems. Cisplatin’s selectivity in killing rapidly proliferating cancer cells is largely dependent on covalent binding to DNA via cisplatin’s chloride sites that had been aquated. We hypothesized that cisplatin’s toxicity in slowly proliferating or terminally differentiated cells is primarily due to drug-protein interactions, instead of drug-DNA binding. To identify proteins that bind to cisplatin, we synthesized two different platinum-agarose conjugates, one with two amino groups and another with two chlorides …